WO2020067590A1 - Puce de protéine pour analyse quantitative - Google Patents

Puce de protéine pour analyse quantitative Download PDF

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Publication number
WO2020067590A1
WO2020067590A1 PCT/KR2018/011507 KR2018011507W WO2020067590A1 WO 2020067590 A1 WO2020067590 A1 WO 2020067590A1 KR 2018011507 W KR2018011507 W KR 2018011507W WO 2020067590 A1 WO2020067590 A1 WO 2020067590A1
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Prior art keywords
quantitative analysis
protein
target protein
bioreceptor
metal
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PCT/KR2018/011507
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English (en)
Korean (ko)
Inventor
최준규
유창혁
Original Assignee
주식회사 스몰머신즈
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Priority to PCT/KR2018/011507 priority Critical patent/WO2020067590A1/fr
Priority to KR1020207012921A priority patent/KR102342302B1/ko
Publication of WO2020067590A1 publication Critical patent/WO2020067590A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the present invention relates to a protein chip, and more particularly, to a protein chip including a plurality of detection units functionally configured to perform a high-precision quantitative analysis of a target protein and a method for quantitative analysis of a target protein using the same.
  • Quantitative analysis methods of target proteins include Enzyme Immunoassay (EI), Enzyme-Linked Immunosorbent Assay (ELISA), Radioimmunoassay (RIA), Fluorescence Immunoassay, FIA , Bioassays such as RNA abundance analysis, and the like are well known.
  • EI Enzyme Immunoassay
  • ELISA Enzyme-Linked Immunosorbent Assay
  • RIA Radioimmunoassay
  • Fluorescence Immunoassay FIA
  • Bioassays such as RNA abundance analysis, and the like are well known.
  • the results of quantitative protein analysis play an important role in areas such as detection of test subjects, human or veterinary diagnostics, forensic diagnostics, environmental analysis, food analysis, and biodefense screening of hazardous substances in the air or in water. can do.
  • the risk caused by the radioactive material may be a problem.
  • the quantitative analysis method of proteins based on the analysis method using the existing ELISA method can obtain only the analysis value for one protein at a time, the consumption of samples, time, labor, and consumables is required to analyze multiple proteins. Not only is it necessary, but it is difficult to trust the result absolutely because it is not analyzed in one sample.
  • the protein chip is a target that immobilizes dozens to hundreds of different proteins, ligands and bio-receptors such as antibodies and antibodies on a solid substrate, and specifically reacts with them. It may be a chip configured to analyze the presence of the protein using an analysis method such as a fluorescence signal meter.
  • the method for quantitative analysis of the target protein based on the protein chip may be performed by analyzing the interaction of the target protein with the bioreceptor disposed on the chip.
  • the main protein in the cell may exist at a single molecular level.
  • detection efficiency may be low.
  • the development of a new level of protein chip to detect and quantify the target protein present in a very small amount This is necessary.
  • the conventional method for quantitative analysis of proteins based on protein chips can be performed by purchasing a protein chip to which a bioreceptor of a target protein is attached, and thus, when the type of target protein increases, an expensive protein chip Due to this, there is a problem that the analysis cost also increases.
  • the inventors of the present invention closely watched for a detection region of a function for substantially detecting a target protein in a protein chip. As a result, the inventors of the present invention were able to develop a new protein chip in which metal was deposited on a protein chip.
  • the inventors of the present invention were able to confirm that the detection portion with a metal spot can detect the target protein with high sensitivity and precision. Furthermore, the inventors of the present invention, in the detection unit, was configured so that the bioreceptor for the target protein to be quantitatively analyzed by the end user for each metal spot.
  • the inventors of the present invention if the area occupied by the metal spot in the same area is the same condition, that is, the area ratio occupied by the metal spot is the same, the smaller the size of the metal spot, the fluorescent signal detected by the fluorescently labeled antibody bound to the metal spot It was confirmed that is increased.
  • the inventors of the present invention can precisely adjust the immobilization density in the sub-detector of the bioreceptor by placing a plurality of sub-detectors configured to gradually increase the density of metal spots in the detector on the protein quantitative analysis chip. It was confirmed that the sensitivity and precision of the quantitative analysis of the target protein were improved than the conventional protein chip.
  • the inventors of the present invention when attaching a bioreceptor for a target protein on a metal spot in the form of coating a self-assembly monolayer (SAM) film on a substrate of a protein chip, the orientation of the bioreceptor increases I could recognize it. Furthermore, the inventors of the present invention were able to confirm that the smaller the size of the metal spot, the more uniform the signal generated according to the binding of the target protein and the bioreceptor and greatly amplified.
  • SAM self-assembly monolayer
  • the inventors of the present invention when a bioreceptor for a specific target protein on a protein chip is fixed with different numbers per unit area, i.e., with different densities, the properties of the bioreceptor having different dissociation factors are determined. It can be recognized that the dynamic range of signals generated according to the immune response of the target protein and antibody can be expanded.
  • the inventors of the present invention were able to recognize that a target protein of the same concentration may have a unique binding amount according to the density of the bioreceptor, and a regression line can be obtained by graphically showing the correlation thereof. As a result, the inventors of the present invention were able to identify a regression line having a unique slope value according to the concentration of the target protein.
  • the inventors of the present invention were able to develop a quantitative analysis method of a target antigen capable of tracking the concentration of the target protein with a slope value of a regression line based on the response signal of the antigen (target protein) versus the density of the antibody.
  • the inventors of the present invention by providing such a method for quantitative analysis of the target antigen, to solve the hassle of the standard protein signal value in a protein quantitative analysis method using a conventional protein chip, and the actual value of the noise signal It was confirmed that distortion can be reduced.
  • the problem to be solved by the present invention is to detect a target protein, a plurality of sub-detection units including a plurality of quantitative analysis units made of metal spots, and performing optical analysis to detect a plurality of quantitative analysis units It is to provide a protein chip capable of quantitative analysis of the target protein.
  • Another problem to be solved by the present invention is to fix a bioreceptor that specifically binds to a target protein in a metal spot of a protein chip, process a sample containing the target protein on a chip for protein quantification, and provide multiple It is to provide a method for quantitative analysis of a target protein, comprising performing a quantitative analysis of the target protein detected in the quantitative analysis unit.
  • Another problem to be solved by the present invention is to provide a kit for quantitative analysis of a target protein, comprising a protein chip and a bioreceptor that specifically binds to a target protein.
  • a protein chip including a substrate includes a plurality of sub-detection units configured to detect a target protein, and each of the plurality of sub-detection units includes a plurality of quantitative analysis units (units) made of metal spots.
  • the protein chip of this configuration is configured to enable quantitative analysis of the target protein detected in a plurality of quantitative analysis units by performing optical analysis.
  • each of the plurality of quantitative analysis units includes a metal spot and a bioreceptor that specifically binds a target protein, each of which may have a different density of metal spots from each other. Furthermore, the bioreceptor can be attached to the metal spot.
  • a plurality of quantitative analysis units are arranged in series within one sub-detection unit, and the plurality of quantitative analysis units arranged in series may be configured to gradually increase the density of the metal spot.
  • each of the plurality of quantitative analysis units may include metal spots having a shape of dots having a predetermined size and disposed in different numbers for each of the plurality of analysis units so as to have different densities from each other. You can.
  • each of the plurality of quantitative analysis units may include metal spots having a polygonal shape and disposed in different sizes for each of the plurality of analysis units to have different densities.
  • the plurality of quantitative analysis units further comprises a linker configured to be attached to the metal spot, and the bioreceptor can be attached to the metal spot by the linker.
  • the protein chip may further include a self-assembled monolayer configured to cover a metal spot on a substrate and attach a linker on the metal spot.
  • it may be a target protein or bioreceptor, or fluorescence-labeled.
  • the metal spots may be arranged on the substrate at regular intervals to form a pattern.
  • the line width of the pattern may be 7 ⁇ m to 12 ⁇ m.
  • the metal spot has a dot shape having a diameter of 500 nm to 10 ⁇ m, and the pattern may be formed by a dot-shaped metal spot.
  • the metal spot may be made of at least one metal from the group consisting of Au, Ni, Cu, Zn, Fe, Al, Ti, Pt, Hg, Ag, Pb and alloys thereof. .
  • the plurality of sub-detection units constitute one of the plurality of detection units, and each of the plurality of detection units may be configured to detect different target proteins from each other.
  • a plurality of sub-detection units may be arranged at intervals of 0.3 mm to 0.9 mm in one detection unit.
  • a method for quantitative analysis of a target protein using a protein chip is provided.
  • the quantitative analysis method the step of immobilizing a bioreceptor that specifically binds to a target protein to a metal spot disposed in a plurality of quantitative analysis units of the protein chip of the present invention, the target protein and the bioreceptor react, the target And processing the sample containing the protein on the chip for protein quantification and performing the quantitative analysis of the target protein detected in the plurality of quantitative analysis units.
  • the target protein or bioreceptor is fluorescently labeled
  • the step of performing quantitative analysis is to quantitatively analyze the target protein by measuring the intensity of fluorescence with respect to subunits composed of a plurality of quantitative analysis units. It may include the step of performing.
  • each of the plurality of quantitative analysis units has a density of different metal spots
  • the step of fixing the bioreceptor is such that the plurality of quantitative analysis units have different densities of the bioreceptors, so that the multiple quantitative analysis And fixing the bioreceptor on the metal spot of the unit.
  • the step of performing the quantitative analysis may include performing a quantitative analysis of the target protein by measuring the amount of binding to the target protein according to the density of the bioreceptor for a plurality of quantitative analysis units.
  • the step of performing quantitative analysis measures a binding amount of a target protein that binds to a bioreceptor having a different density from each other, graphically displays the correlation thereof, obtains a regression line, and obtains the slope value thereof It may include the step of performing a quantitative analysis of the target protein.
  • the step of performing the quantitative analysis may include performing a quantitative analysis of a target protein using at least one method selected from the group consisting of mass spectrometry.
  • the method for quantitative analysis of a target protein further comprises placing a linker that indirectly connects the bioreceptor and the metal spot on the spot, performed before the step of immobilizing the bioreceptor.
  • fixing the bioreceptor may include fixing the bioreceptor on a metal spot disposed in a plurality of quantitative analysis units through a linker.
  • the step of placing the linker on the metal spot comprises a linker and a self-assembleable molecule to cover the metal spot on the substrate of the protein chip and attach the linker on the metal spot. Coating the protein chip onto a plurality of quantitative analysis units using a coating solution.
  • kits for quantitative analysis of a target protein which includes a protein chip according to an embodiment of the present invention.
  • the kit includes the protein chip of the present invention and a bioreceptor that specifically binds to the target protein.
  • the kit for quantitative analysis of proteins may further include a buffer solution.
  • the present invention has an effect capable of detecting a very small amount of target protein with high sensitivity from a small amount of sample by providing a protein chip equipped with a sub-detecting unit including a quantitative analysis unit formed by a metal spot.
  • the present invention if the conditions of the same area occupied by the metal spots in the same region, that is, the area ratios of the metal spots are the same, the smaller the size of the metal spot, the more the fluorescent signal detected by the fluorescently labeled antibody bound to the metal spot.
  • the increased phenomenon unlike DNA strands that can be replicated upon detection and obtain the effect of signal amplification, it has the effect of signal amplification in detecting proteins that are not capable of replication.
  • the present invention precisely fixes the immobilization density in the sub-detector of the bioreceptor by placing a sub-detector comprising a plurality of quantitative analysis units configured to gradually increase the density of metal spots in the detection section on the protein quantitative analysis chip. Can be adjusted.
  • the present invention has an effect capable of providing a quantitative analysis result with improved sensitivity and precision than a conventional protein chip.
  • the present invention has an effect capable of overcoming the limitations of a conventional protein chip, in which a bioreceptor for detecting a specific protein is fixed.
  • the present invention provides an effect of reducing an analysis cost by configuring a bioreceptor for a target protein to be quantitatively analyzed by an end user for each metal spot present in the detection unit. You can. Furthermore, the present invention has an effect capable of providing quantitative analysis for a plurality of target proteins through one analysis.
  • the bioreceptors for a specific target protein can be immobilized at different numbers per unit area, i.e., at different densities, to have different dissociation factors.
  • the dynamic range of the resulting signal can be enlarged.
  • the present invention can provide a method for quantitative analysis of a target antigen capable of tracking the concentration of a target protein with a slope value of a regression line based on a response signal of the antigen (target protein) to the density of the antibody.
  • the present invention is easier than the conventional method, as it solves the hassle associated with the standard protein signal value in the protein quantitative analysis method using a conventional protein chip, and reduces the distortion of the actual value due to the noise signal. And has the effect of performing accurate quantitative analysis.
  • 1A is a schematic plan view of a protein chip according to an embodiment of the present invention.
  • 1B is an exemplary view showing a detection unit, a sub-detection unit, and a quantitative analysis unit of a protein chip according to an embodiment of the present invention.
  • 1C exemplarily shows a quantitative analysis unit that is gradually disposed according to the density of a metal spot in a sub-detection unit of a protein chip according to an embodiment of the present invention.
  • Figure 1d shows the results of the analysis of the fluorescence intensity measured according to the density of the metal spot disposed on the substrate.
  • 1E to 1G exemplarily illustrate a quantitative analysis unit that is gradually disposed according to the density of a metal spot in a sub-detection unit of a protein chip according to various embodiments of the present invention.
  • 1H to 1J illustrate that a bioreceptor is fixed to a quantitative analysis unit of a protein chip according to an embodiment of the present invention.
  • FIG. 2 exemplarily shows a detection unit, a sub-detection unit, and a quantitative analysis unit of a protein chip according to another embodiment of the present invention.
  • 3A and 3B exemplarily show a region separation membrane used in a protein chip according to various embodiments of the present invention.
  • FIG. 4 shows a procedure of quantitative analysis of a target protein using a protein chip according to an embodiment of the present invention.
  • Figure 5a shows the results of the analysis of the intensity of the signal along the surface of one metal spot for the protein chip according to an embodiment of the present invention.
  • Figure 5b shows the results of analyzing the signal intensity according to the density of the antibody and the signal amplification effect according to the metal spot size, for the protein chip according to an embodiment of the present invention.
  • Figure 5c shows the results of the analysis of the slope value of the regression line according to the concentration of the antigen for the protein chip according to an embodiment of the present invention.
  • the protein chip according to an embodiment of the present invention is configured to enable quantitative analysis of a target protein detected in a plurality of quantitative analysis units by performing optical analysis.
  • the substrate of the protein chip includes a plurality of sub-detection units configured to detect a target protein, and each of the plurality of sub-detection units includes a plurality of quantitative analysis units made of metal spots.
  • protein chip may mean a single chip configured to separate, detect and quantify a target protein.
  • a bioreceptor configured to react with a target protein may be fixed on a substrate made of a polymer such as glass, silicon, silicon, polypropylene, or the like.
  • the protein chip disclosed in this specification may be interpreted in the same way as a protein chip for quantitative analysis, a slide for protein array, and a protein quantitative chip.
  • the protein chip in the present specification may encompass a chip in which the bioreceptor is not fixed or a chip in which the bioreceptor for a specific protein is fixed.
  • the substrate of the protein chip may be made of a material selected from the group consisting of glass, silicon, polypropylene, nylon, plastic, and metal, but is not limited thereto.
  • target protein may mean a protein to be present in a sample and a quantitative analysis using a protein chip.
  • the target protein may be a biomarker of an antibody, antigen, enzyme, or peptide unit, but is not limited thereto.
  • bioreceptor can mean any substance that interacts with a target protein.
  • the bioreceptor may be at least one of proteins, ligands, nucleic acids, carbohydrates, and antibodies that interact with the target protein, but is not limited thereto and may be variously set according to the properties of the target protein.
  • the bioreceptor can be an antibody.
  • the bioreceptor may be an antibody targeting one target protein and having different dissociation factors.
  • the protein chip of the present invention may be capable of quantitative analysis of a wider concentration region for one target protein.
  • the target protein or bioreceptor can be a fluorescence-labeled receptor.
  • the fluorescently labeled target protein may be a protein to which a fluorochrome that generates fluorescence by light stimulation is bound before being processed on the protein chip of the present invention.
  • the fluorescently labeled bioreceptor may be a receptor bound with a pigment that generates fluorescence.
  • the fluorescent pigment may be a pigment protein of fluorescein isothiocyanate (FITC), rhodamine isothiocyanate (RITC) or picoerythrin that emits red-orange fluorescence, but is not limited thereto. .
  • the user quantifies the target protein by detecting a signal according to the interaction of the bioreceptor and the target protein in the protein chip using an analysis method such as fluorescence antibody method, flow cytometry, immunofluorescence method, etc. Analysis can be performed.
  • the bioreceptor used for mass spectrometry of a target protein in the present specification is not limited to a fluorescently labeled receptor.
  • a bioreceptor without a fluorescent dye SPR imaging analysis, surface-enhanced laser desorption-ionization (SELDI) mass spectrometry, atomic force microscope (AFM) analysis and matrixassisted laser MALDI-TOF Desorption / ionization time-of-flight
  • SEDM surface-enhanced laser desorption-ionization
  • AFM atomic force microscope
  • MALDI-TOF Desorption / ionization time-of-flight matrixassisted laser MALDI-TOF Desorption / ionization time-of-flight
  • the term “sub-detection unit” may mean a region configured to detect a target protein on a protein chip.
  • the sub-detection unit may be composed of a plurality of quantitative analysis units (units) including a plurality of metal spots (spots) formed by depositing metal on a substrate of a protein chip.
  • the sub-detection unit may mean all regions in which metal spots are present in the protein chip.
  • one detection unit may be formed.
  • the protein chip of the present invention may include a plurality of independent detection units. Accordingly, quantitative analysis of target proteins of multiple samples may be performed through one analysis.
  • the term “quantitative analysis unit” may mean a unit in which detection and quantitative analysis of a target protein are substantially performed in a sub-detection unit. At this time, the aforementioned metal spots may be disposed in the quantitative analysis unit.
  • metal spot may refer to spots formed by depositing metal on a substrate of a protein chip as described above. At this time, a bioreceptor according to the target protein may be attached on the metal spot.
  • a linker may be attached to the metal spot, and the bioreceptor may be fixed to the metal spot through the linker.
  • the linker may be a protein A / G, dextran (dextran) or PEG (polyethylene glycol), but is not limited thereto.
  • the linker can be attached to the metal spot surface by coating the coating solution comprising the linker and the self-assembleable molecule in a single layer on a quantitative analysis unit of the protein chip.
  • a self-assembled monolayer (SAM) can be formed on the substrate of the protein chip.
  • each of the plurality of quantitative analysis units may include metal spots having a dot shape of a certain size and disposed in different numbers for each of the plurality of analysis units to have different densities.
  • each of the plurality of quantitative analysis units may include a metal spot having a polygonal shape and disposed in different sizes for each of the plurality of analysis units so as to have different densities.
  • the metal spot may have a dot shape having a unit of 10 ⁇ m from 500 nm, but may have various shapes as long as the metal is deposited to have a constant area on the substrate of the protein chip.
  • the diameter of the dot-shaped metal spot may be 500 nm to 10 ⁇ m.
  • the signal amplification effect may be obtained, but the diameter of the metal spot may be 500 nm to 1 ⁇ m, but is not limited thereto.
  • the line width of the formed pattern may be 7 ⁇ m to 12 ⁇ m, but is not limited thereto.
  • the metal spot may be at least one of the group consisting of Au, Ni, Cu, Zn, Fe, Al, Ti, Pt, Hg, Ag, Pb and alloys thereof.
  • the metal spot disclosed in the present specification may be gold, but is not limited thereto, and may be variously set according to the type of target protein and the type of bioreceptor.
  • the detection unit may include a plurality of sub-detection units on which metal spots capable of attaching bioreceptors according to target proteins are disposed.
  • sub detection unit may mean a subunit of the detection unit.
  • the sub-detection units may be arranged at intervals of 0.3 mm to 0.9 mm in one detection unit. However, it is not limited thereto.
  • a plurality of quantitative analysis units may be arranged in series, within one sub-detection unit, and the plurality of quantitative analysis units arranged in series may be configured to gradually increase or decrease the density of metal spots.
  • the detection portion of the protein chip of the present invention may be composed of sub-detection portions including a quantitative analysis unit configured to gradually change the density of the metal spot.
  • the change in density of the metal spot may mean a change in density of the bioreceptor.
  • the signal according to the reaction between the bioreceptor and the target protein may increase in direct proportion. That is, the target protein may have a unique binding amount (unique slope value) according to the concentration of the bioreceptor depending on its type. Therefore, as the user can estimate the concentration of the target protein by using the slope value of the target protein in reverse, quantitative analysis of the target protein may be possible.
  • the quantitative analysis method using the unique slope value can reduce an error in analysis due to enhancement or reduction of a signal due to equipment or other environmental factors.
  • the user can perform quantitative analysis with improved precision and sensitivity to the target protein by using only one detection unit among a plurality of detection units and one single analysis.
  • the detection unit configured to detect the target protein in the protein chip is composed of sub-units composed of metal spots having a certain density
  • multiple iterative experiments may be required for high-precision and sensitive quantitative analysis of the target protein. have. That is, when using a conventional protein chip with a fixed bioreceptor having a certain density, quantitative analysis with high precision and sensitivity to a target protein may be difficult. Furthermore, such a conventional protein chip may require a reference signal value of a standard protein for quantitative analysis.
  • the protein chip may further include a self-assembled monolayer disposed on the detection unit.
  • the user can identify a plurality of different target proteins in independent detection regions using a single protein chip, and perform quantitative analysis on each target protein.
  • sample may mean a sample containing a target protein.
  • the analysis sample can be a fluid sample.
  • fluid sample for example, cell lysate, whole blood, plasma, serum, saliva, ocular fluid, cerebrospinal fluid, sweat, urine, milk, ascites fluid, synovial fluid, and peritoneal fluid, but are not limited thereto.
  • the sample can be easily selected by the user according to the purpose of use of the protein chip of the present invention.
  • the sample may be lysed prior to being processed on the protein chip of the present invention, depending on its type.
  • 1A is a schematic plan view of a protein chip according to an embodiment of the present invention.
  • the protein chip 100 may include a plurality of detection units 110 and a barcode unit 120.
  • the detection unit 110 may include a plurality of sub-detection units 112.
  • the sub-detection unit 112 may include a plurality of quantitative analysis units 112.
  • the protein chip 100 may be configured to detect different target proteins for each of the plurality of detection units 110, so that a user can detect a plurality of target proteins using one protein chip 100 and Quantitative analysis can be performed. Meanwhile, the user can check information on each protein chip 100 through the barcode unit 120. For example, when there are many types of samples to be analyzed, the user may classify the type of the protein chip 100 through the barcode unit 120.
  • 1B is an exemplary view showing a detection unit, a sub-detection unit, and a quantitative analysis unit of a protein chip according to an embodiment of the present invention.
  • one detection unit 110 may include a plurality of sub-detection units 112.
  • the sub-detecting unit 112 is formed by depositing metal on the substrate constituting the protein chip 100, and a plurality of quantitative analysis units 112 (a), 112 (b), and 112 (c) having different metal spot densities, respectively. ), 112 (d), 112 (e), 112 (f), 112 (g) and 112 (h)).
  • a plurality of quantitative analysis units 112 (a), 112 (b), 112 (c), 112 (d), 112 (e), 112 (f), 112 (g) and 112 (h)) are one Within the sub-detector 112, may be arranged in series (e.g., 112 (a), 112 (b), 112 (c) and 112 (d), or 112 (e), 112 (f) , 112 (g) and 112 (h)), a plurality of quantitative analysis units arranged in series (e.g., 112 (a), 112 (b), 112 (c) and 112 (d), Or 112 (e), 112 (f), 112 (g) and 112 (h)) may increase the density of metal spots gradually.
  • a bioreceptor according to the target protein may be attached on the metal spot. That is, as the density of metal spots gradually increases, a plurality of quantitative analysis units 112 (a), 112 (b), 112 (c), 112 (d), 112 (e), 112 (f), 112 (g) and 112 (h)), the concentration of the bioreceptor can also be gradually increased.
  • bio Quantitative analysis of the target protein may be performed by measuring a signal according to the reaction between the receptor and the target protein.
  • the signal according to the reaction between the bioreceptor and the target protein may increase in direct proportion. That is, the target protein may have a unique binding amount (unique slope value) according to the concentration of the bioreceptor depending on its type. Therefore, as the user can estimate the concentration of the target protein by using the slope value of the target protein in reverse, quantitative analysis of the target protein may be possible.
  • the quantitative analysis method using the unique slope value can reduce an error in analysis due to enhancement or reduction of a signal due to equipment or other environmental factors.
  • 1C exemplarily shows a quantitative analysis unit that is gradually disposed according to the density of a metal spot in a sub-detection unit of a protein chip according to an embodiment of the present invention.
  • the quantitative analysis unit 112 (a), the density of the metal spot 114 (a) is 20%, the metal spot 114 (b) Quantitative analysis unit 112 (d) with a density of 50% and quantitative analysis unit 112 (h) with a density of metal spot 114 (c) of 90% are shown.
  • the quantitative analysis units 112 (a), 112 (d) and 112 (h) have different metal spots 114 (a), 114 (b) and 114 (c) for the same area. It can be arranged in number. Accordingly, the quantitative analysis units 112 (a), 112 (d), and 112 (h) are sub-detectors such that the density of the metal spots 114 (a), 114 (b), and 114 (c) is gradually increased. It can be configured within 112. For example, referring to FIG. 1D together, an analysis result of the measured fluorescence intensity appears after differently arranging the density of metal spots on the substrate. More specifically, it appears that the intensity of fluorescence gradually increases or decreases depending on the reaction with the target protein according to the density of the metal spot.
  • the metal spots 114 (a), 114 (b), and 114 (c) are attached with a bioreceptor that reacts with the target protein
  • the density of each of the metal spots 114 (a), 114 (b) and 114 (c) of 112 (h)) may correspond to the density (or concentration) of the bioreceptor.
  • the user can perform quantitative analysis on the target protein by using only one detection unit 110 among the plurality of detection units 110 and only one single analysis.
  • the metal spots 114 (a), 114 (b) and 114 (c) are, in dot form, at regular intervals within the quantitative analysis units 112 (a), 112 (d) and 112 (h).
  • a specific pattern may be formed, wherein the line width of the formed gold pattern may be 10 ⁇ m, however, depending on the shape of the metal spot disposed in the quantitative analysis unit and the area of each quantitative analysis unit.
  • the method for setting the density of the metal spot is not limited to the above.
  • 1E to 1G exemplarily illustrate a quantitative analysis unit that is gradually disposed according to the density of a metal spot in a sub-detection unit of a protein chip according to various embodiments of the present invention.
  • a quantitative analysis unit 112 (d) with a density of 50% and a quantitative analysis unit 112 (h) with a density of metal spot 114 '(c) of 90% is shown.
  • the metal spots 114 '(a), 114' (b), and 114 '(c) in the form of rectangular rods are attached to the quantitative analysis units 112 (a), 112 (d) and 112 (h). Accordingly, it can be arranged in different numbers. Thus, each of the quantitative analysis units 112 (a), 112 (d) and 112 (h), the total metal spots 114 '(a), 114' (b) and 114 '(c) ))) As the area occupied by these may differ, each may have a different density.
  • a quantitative analysis unit 112 (a) having a density of 20% of the metal spot 114 '' (a), and a metal spot 114 '' Quantitative analysis units 112 (d) with a density of (b)) of 50% and quantitative analysis units 112 (h) with a density of metal spots 114 '' (c) of 90% are shown.
  • the square-shaped metal spots 114 '' (a), 114 '' (b) and 114 '' (c)) are quantitative analysis units 112 (a), 112 (d) and 112 (h). ) May have a different area.
  • the quantitative analysis unit 112 (a) having a density of the metal spot 114 '' '(a) is 20%
  • quantitative analysis unit 112 (h) with a density of metal spot 114 '' '(c) of 90% is shown. do.
  • the metal spots 114 '' '(a), 114' '' (b) and 114 '' '(c)) are one quantitative analysis unit 112 (a), 112 (d), or 112 ( h)). Furthermore, the metal spots 114 '' '(a), 114' '' (b) and 114 '' '(c)) are quantitative analysis units 112 (a), 112 (d) and 112 (h)) It may be arranged to have a different area.
  • 1H to 1J illustrate that a bioreceptor is fixed to a quantitative analysis unit of a protein chip according to an embodiment of the present invention.
  • a bioreceptor 118 such as an antibody
  • a linker molecule 116 can be attached onto a metal spot 114 through a linker molecule 116.
  • the linker molecule 116, the coating solution containing the linker molecule 116 and self-assembleable molecule is a single on the quantitative analysis unit 112 (a), 112 (d) and 112 (h) of the protein chip By being coated with a layer, it can be attached to the metal spot 114 surface.
  • the bioreceptor 118 can be stably fixed on the biochip by the coating of the single molecular layer 119, and may have orientation.
  • the substrate of the protein chip 100 is It may be covered with a single molecular layer 119, and a linker 116 may be attached to the metal spot 114. Thereafter, the bioreceptor 118 according to the target protein may be combined through an additional reaction.
  • the protein chip disclosed in the present specification is not limited to the above-described form.
  • a bioreceptor 118 such as an antibody, can be attached directly onto the metal spot 114.
  • the bioreceptor 118 is coated with a single layer on a quantitative analysis unit 112 (a), 112 (d), and 112 (h) of the protein chip by coating a coating solution containing self-assembleable molecules, thereby forming a metal spot. (114) may be attached to the surface.
  • the bioreceptor 118 can be stably fixed on the biochip by the coating of the single molecular layer 119, and may have orientation.
  • the bioreceptor 118 may be directly attached to the metal spot 114 without coating of a single molecular layer.
  • FIG. 2 exemplarily shows a detection unit, a sub-detection unit, and a quantitative analysis unit of a protein chip according to another embodiment of the present invention.
  • the protein chip 200 comprises a plurality of detection units 210, a plurality of sub-detection units 212 forming the detection unit 210, and a sub-detection unit 210 It includes a plurality of quantitative analysis units 212 (a), 212 (b), 212 (c), 212 (d) and 212 (e).
  • the shape of the detection unit formed on the protein chip is not limited thereto.
  • the protein chip 300 may include one detection unit 310 formed on a substrate.
  • a plurality of detection units 310 may be formed by arranging region separation membranes 320 ′ and 320 ′′, which provide independent detection regions on the protein chip 300.
  • the region separation membranes 320 ′ and 320 ′′ may be in a detachable form on the detection unit 310 of the protein chip 300.
  • FIG. 4 shows a procedure of quantitative analysis of a target protein using a protein chip according to an embodiment of the present invention.
  • the quantitative analysis method of the target protein first, to fix the bioreceptor that specifically binds to the target protein on the detection portion of the protein chip of the present invention (S410), the sample protein containing the target protein It is configured to process on a chip (S420) and perform quantitative analysis of the detected target protein (S430).
  • the bioreceptor in the step of fixing the bioreceptor (S410), the bioreceptor is placed on the metal spots of the plurality of quantitative analysis units such that the plurality of quantitative analysis units have different densities of bioreceptors. Can be fixed.
  • each of the plurality of quantitative analysis units may have a different density of metal spots.
  • the bioreceptor in the step of fixing the bioreceptor (S410), the bioreceptor may be fixed on a metal spot of the plurality of quantitative analysis units such that the plurality of quantitative analysis units have different densities of bioreceptors.
  • a linker that indirectly connects the bioreceptor and the metal spot is performed before the step of fixing the bioreceptor (S410) on the metal spot.
  • the step of placing may be further performed.
  • the bioreceptor may be attached to a metal spot disposed in a plurality of quantitative analysis units through a linker.
  • the linker covers a metal spot on the substrate of the protein chip, and a coating solution configured to coat the linker on the metal spot is coated, thereby allowing a plurality of quantitative analysis units of the protein chip It can be attached to.
  • the bioreceptor used in the step (S410) of fixing the bioreceptor may be a fluorescently labeled receptor, but is not limited thereto.
  • step (S420) of processing a sample containing the target protein on a protein quantitative analysis chip interaction of the target protein and the bioreceptor attached on the metal spot may occur.
  • a step (S420) of processing a sample containing a target protein on a protein quantitative analysis chip an immune response of the fluorescently labeled bioreceptor and the target protein occurs, and a fluorescent signal may be generated by the immune response.
  • a fluorescence signal may be generated as the immune response of the bioreceptor and the pre-fluorescence-labeled target protein occurs.
  • the quantitative analysis of the target protein may be performed by measuring the intensity of fluorescence with respect to a subunit composed of a plurality of quantitative analysis units.
  • the quantitative analysis of the target protein is performed by measuring the amount of binding to the target protein according to the density of the bioreceptor for a plurality of quantitative analysis units This can be done.
  • the signal according to the reaction between the bioreceptor and the target protein may increase in direct proportion. That is, the target protein may have a unique binding amount (unique slope value) according to the concentration of the bioreceptor depending on its type. Therefore, as the user can estimate the concentration of the target protein by using the slope value of the target protein in reverse, quantitative analysis of the target protein may be possible.
  • the method for quantitative analysis of a target protein according to various embodiments of the present invention using such a unique slope value can reduce an error in analysis due to enhancement or reduction of a signal due to equipment or other environmental factors.
  • step (S430) of performing a quantitative analysis of the target protein SPR imaging (imaging) analysis, surface-enhanced laser desorption-ionization (SELDI) mass spectrometry, atomic force microscope (AFM) analysis and Quantitative analysis of the target protein may be performed using at least one method selected from the group consisting of MALDI-TOF (Matrixassistedlaser desorption / ionization time-of-flight) mass spectrometry.
  • MALDI-TOF Microxassistedlaser desorption / ionization time-of-flight
  • Figure 5a shows the results of the analysis of the intensity of the signal along the surface of one metal spot for the protein chip according to an embodiment of the present invention.
  • a linker is attached by SAM coating on a protein chip on which a metal pattern is formed by deposition of a metal spot, and then a fluorescently labeled antibody for the target protein is fixed. Then, a fluorescence signal according to the size of the line width is measured using a scanner.
  • the measured fluorescence signal appears uniformly. This may mean that the signal on the inner surface of the protein chip appears uniformly when the antibody is tightly immobilized.
  • the line width of the gold pattern formed on the protein chip of the present invention may be set to 7 to 12 ⁇ m.
  • the standard deviation of the signal between the metal spots in the protein chip of the present invention is 2% or less, and the standard deviation of the neoplasm between the protein chips may be 5% or less, the bioreceptor antibody is stably and uniformly fixed. As a result, the immobilization efficiency in the protein chip can be increased.
  • Figure 5b shows the results of analyzing the signal intensity according to the density of the antibody and the signal amplification effect according to the metal spot size, for the protein chip according to an embodiment of the present invention.
  • the area occupied by the metal spot in the same area is the same condition, that is, the area ratio of the metal spot is the same, the smaller the size of the metal spot, the more the fluorescence bound to the metal spot.
  • the fluorescent signal detected by the labeled antibody increases and the area ratio occupied by the metal spot increases, that is, the density (concentration) of the bioreceptor fixed on the metal spot increases, the intensity of the fluorescent signal according to the reaction with the target protein Appears to increase in direct proportion.
  • the target protein may have a unique binding amount (unique slope value) according to the concentration of the bioreceptor, depending on its type. Therefore, as the user can estimate the concentration of the target protein by using the slope value of the target protein in reverse, quantitative analysis of the target protein may be possible.
  • the quantitative analysis method using the unique slope value can reduce an error in analysis due to enhancement or reduction of a signal due to equipment or other environmental factors.
  • Figure 5c shows the results of the analysis of the slope value of the regression line according to the concentration of the antigen for the protein chip according to an embodiment of the present invention.
  • DNA-Cy5 shows a slope value of about 179.3 formed by the fluorescent signal measured according to an increase in the density of the bioreceptor.
  • DNA-Cy5 of 10 nM shows a slope value of about 31.603 formed by the fluorescent signal measured according to an increase in the density of the bioreceptor.
  • the target proteins appear to have a unique slope value depending on their concentration and their kind.
  • the user can estimate the concentration of the target protein using the inclination value of the target protein (eg, 179.3 or 31.603) in reverse, quantitative analysis of the target protein may be possible.
  • the inclination value of the target protein eg, 179.3 or 31.603
  • the protein chip used in various embodiments of the present invention can detect a very small amount of target proteins from a small amount of samples with high sensitivity, and perform quantitative analysis with high sensitivity and precision for these target proteins.
  • the present invention provides a kit including a protein chip and a bioreceptor, so that a user can perform efficient quantitative analysis on a variety of proteins.
  • the present invention by placing a plurality of sub-detectors configured to gradually increase the density of metal spots in the detection section on the protein quantitative analysis chip, it is possible to provide a quantitative analysis result with improved sensitivity and precision than the conventional protein chip It works.
  • the present invention has an effect capable of overcoming the limitations of a conventional protein chip, in which a bioreceptor for detecting a specific protein is fixed.
  • the present invention when immobilizing a bioreceptor for a specific target protein, a bioreceptor having different dissociation factors, for example, immobilizing an antibody, dynamic range of signals generated according to an immune response of the target protein and the antibody (dynamic range) can be enlarged.
  • the present invention has the effect of reducing the number of repetition experiments required in a biological experiment, and tracking the concentration of the target protein based on the reaction signal of the antigen (target protein) against the density of the antibody.
  • the present invention has the effect of solving the hassle of accommodating standard protein signal values in a protein quantitative analysis method using a conventional protein chip, and performing quantitative analysis more easily than a conventional method.

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Abstract

La présente invention concerne une puce de protéine et un procédé l'utilisant pour analyser quantitativement une protéine cible. La puce de protéine comprend un substrat comprenant une pluralité de sous-parties de détection configurées pour détecter la protéine cible, chacune des sous-parties de la pluralité de sous-parties de détection comprenant une pluralité d'unités d'analyse quantitative composées de points métalliques et la puce de protéine est configurée de telle sorte que la protéine cible détectée par la pluralité d'unités d'analyse quantitative peut être analysée de manière quantitative par réalisation d'une analyse optique.
PCT/KR2018/011507 2018-09-28 2018-09-28 Puce de protéine pour analyse quantitative WO2020067590A1 (fr)

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KR20100006842A (ko) * 2008-07-10 2010-01-22 한국과학기술원 금속―단백질 격자패턴 바이오칩 및 그 제조방법
KR20100124870A (ko) * 2009-05-20 2010-11-30 한국생명공학연구원 산화아연 나노구조체 마이크로패턴 및 그 제작방법
KR20120029144A (ko) * 2010-09-16 2012-03-26 한국과학기술원 혼합된 자기조립 단분자 막을 이용한 단백질 결합 방법 및 그 방법을 이용한 단백질 칩
KR20170011364A (ko) * 2015-07-22 2017-02-02 주식회사 스몰머신즈 분자 고정화 패턴 및 그를 형성하는 방법

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KR20100063316A (ko) * 2008-12-03 2010-06-11 한국전자통신연구원 바이오 물질 검출 장치 및 이를 이용한 바이오 물질 검출 방법
KR101288244B1 (ko) * 2010-03-19 2013-07-26 가천대학교 산학협력단 바이오칩

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Publication number Priority date Publication date Assignee Title
US20080020409A1 (en) * 2004-03-03 2008-01-24 Michael Pawlak Analytical Platform and Method for Generating Protein Expression Profiles of Cell Populations
KR20100006842A (ko) * 2008-07-10 2010-01-22 한국과학기술원 금속―단백질 격자패턴 바이오칩 및 그 제조방법
KR20100124870A (ko) * 2009-05-20 2010-11-30 한국생명공학연구원 산화아연 나노구조체 마이크로패턴 및 그 제작방법
KR20120029144A (ko) * 2010-09-16 2012-03-26 한국과학기술원 혼합된 자기조립 단분자 막을 이용한 단백질 결합 방법 및 그 방법을 이용한 단백질 칩
KR20170011364A (ko) * 2015-07-22 2017-02-02 주식회사 스몰머신즈 분자 고정화 패턴 및 그를 형성하는 방법

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