WO2020062917A1 - Pesticide combination containing microbial compound, preparation method therefor and application thereof - Google Patents

Abstract

Disclosed is a pesticide combination containing a microbial compound, comprising the following active ingredients in parts by weight: 1 to 2 parts of Bacillus cereus, 1 to 2 parts of thiamethoxam, and 5 to 25 parts of Beauveria bassiana. The present pesticide combination containing a microbial compound can be made into a wettable powder, a water dispersible granule, an emulsifiable concentrate, a micro-emulsion, a suspension, or an oil dispersion, can be used for preventing and treating the diseases and insect pests of rice, peanut, soybean, potato, citrus, sugar beet, rape, chili, cucumber, and eggplant, and has the function of preventing and treating Lepidoptera, Coleoptera, Homoptera, Diptera pests, underground pests, and soil-borne bacterial diseases on vegetables. The present pesticide combination containing a microbial compound can reduce the usage amount of a pesticide and delay drug resistance, and has a better prevention and treatment effect than use of a single agent, and the farmers' costs are reduced.

Description

Pesticide composition containing microbial bacteria compound, preparation method and application thereof Technical field

The invention relates to the technical field of pesticides, in particular to a pesticide composition containing microbial bacteria and a preparation method and application thereof.

Background technique

Beauveria bassiana is a broad-spectrum insect pathogenic fungus, and is mainly used to control agricultural and forest pests such as Lepidoptera, Hemiptera, Homoptera, Coleoptera and so on. Beauveria bassiana can effectively control the population of insects, while not harming other natural enemy insects and beneficial organisms, and has no pollution to the environment and does not produce resistance, but the effect of single use is not stable and is greatly affected by environmental conditions. The preparation is a method to solve the unstable effect.

Bacillus cereus is a low-toxicity, low-residue, non-polluting, and safe-to-use microbial pesticide. It is mainly used to control soil-borne bacterial diseases such as ginger blast, eggplant bacterial wilt, and pepper bacterial wilt. It is used for the control of rice oryzae and root-knot nematodes. Through the SOD enzymes in the body, Bacillus cereus can improve the ability of crops to eliminate pathogenic organisms and stress caused by the production of oxygen, adjust the microhabitat of crop cells, maintain the normal physiological metabolism and biochemical reactions of cells, improve stress resistance, and accelerate growth To improve yield and quality.

Thiamethoxam is a second-generation neonicotinoid, a highly effective and low-toxicity insecticide. Its mechanism of action is to selectively inhibit the nicotinic acetylcholinease receptor in the central nervous system of insects, thereby blocking the normal conduction of the central nervous system in insects, causing insects to appear. Paralyzed death, with contact killing, stomach poisoning, systemic activity. Thiamethoxam has a broad insecticidal spectrum, high efficiency and safety, and has high activity against Lepidoptera, Diptera, Coleoptera, Homoptera, and can be used for stem and leaf treatment, seed treatment, and soil treatment. However, long-term use of a single agent is likely to cause resistance to pests.

With the development of national conditions and the needs of the ecological environment, it has become a trend to develop efficient, safe and environmentally friendly green pesticides. Beauveria bassiana, Bacillus cereus, and thiamethoxam are all highly effective and low-toxic pesticides. Enlarging insecticidal spectrum and bactericidal and nematicidal functions. According to different formulations, it can be used for stem and leaf treatment, seed treatment, soil treatment, reducing the amount and frequency of pesticide use, reducing agricultural capital, and at the same time delaying the resistance of the compound preparation to drug resistance. Play a role.

At present, no related compound pesticide composition of Beauveria bassiana, Bacillus cereus, and thiamethoxam has been found.

Summary of the Invention

The purpose of the present invention is to provide a pesticide composition containing microbial bacteria, and compounding Beauveria bassiana, Bacillus cereus and thiamethoxam to form a novel pesticide composition containing microorganisms. The pesticide composition containing microbial bacteria can effectively prevent soil-borne bacterial diseases on the agriculture Lepidoptera, Coleoptera, Homoptera, Diptera pests, underground pests and vegetables, and the performance of the composition is stable. Reducing the use of pesticides and delaying drug resistance, the control effect is better and the cost is lower than single-agent use.

To achieve the above object, the technical solution of the present invention is:

The microbial bacteria-containing compounded pesticide composition of the present invention includes the following active ingredients by weight: 1 to 2 parts of Bacillus cereus, 1 to 2 parts of thiamethoxam, and 5 to 25 parts of Beauveria bassiana.

Further, the compounded microbial-containing pesticide composition includes the following active ingredients by weight: 1.2 to 1.8 parts of Bacillus cereus, 1.2 to 1.8 parts of thiamethoxam, and 15 to 20 parts of Beauveria bassiana.

Further, the pesticide composition containing microbial bacteria is formulated with a solvent and an auxiliary material to prepare a liquid preparation, and the auxiliary materials are an emulsifier, a dispersant, a thickener, a defoaming agent, an antifreeze agent, and a pH adjuster. One or a mixture of two or more of them.

Further, the solvent is methanol, ethanol, ethylene glycol, acetonitrile, toluene, xylene, acetone, N, N-dimethylformamide, dimethyl sulfoxide, epoxy soybean oil, methyl oleate, nordox De Rosin vegetable oil mixed with one or two kinds;

The emulsifiers are calcium dodecylbenzenesulfonate, sodium dodecylbenzenesulfonate, phenethylphenol polyoxyethylene ether, sorbitan monolaurate, fatty acid polyoxyethylene phosphate, One or two or more of alkylphenol polyoxyethylene ether, sodium alkylphenol polyoxyethylene ether sodium sulfate, and polysorbate are mixed;

The dispersant is sodium methylene perylene naphthalene sulfonate, lignin sulfonate perylene naphthalene sulfonate formaldehyde condensate, polyoxyethylene ether carboxylate, alkylphenol polyoxyethylene ether phosphate, and naphthalenesulfonic acid. One or two or more of formaldehyde condensate sodium salt and polyoxyethylene ether sulfonate;

The thickener is polyvinyl alcohol, xanthan gum, polyvinylpyrrolidone, carboxymethyl cellulose, methyl cellulose, carboxyethyl cellulose, gum arabic, sodium polyacrylate, organic bentonite, and fumed silica. Or one or two or more of magnesium aluminum silicate;

The defoaming agent is one of polymerized glycerin, silicone, polyoxypropylene glyceryl ether, and phenylethanol oleate;

The pH adjusting agent is one of triethanolamine, citric acid, sodium acetate, sodium hydroxide, and calcium hydroxide.

Further, the pesticide composition containing the microorganism and bacteria is compounded with an auxiliary material to form a solid preparation, and the auxiliary material is one or a mixture of one or two of an inert filler, a wetting agent, a dispersant, and a disintegrant. .

Further, the method for preparing the solid preparation of the pesticide composition containing microbial bacteria compound includes the following steps:

Mix each raw material and inert filler, pulverize into fine powder, add wetting agent, dispersant, disintegrant, mix well to make powder;

Or on the basis of powder, add appropriate amount of binder to further prepare granules.

Further, the inert filler is one or a mixture of two or more of diatomite, clay, talc, high viscosity starch, kaolin, clay, bentonite, attapulgite, white carbon black, and light calcium carbonate;

The wetting agent is sodium lauryl sulfate, sodium butylnaphthalenesulfonate, sodium alkylbenzenesulfonate, sodium fatty alcohol sulfate, sodium ligninsulfonate, sodium methylenebisnaphthalenesulfonate, and lauryl alcohol. One or two or more of sodium ether sulfate, sodium fatty acid polyoxyethylene ether sulfonate, calcium dodecyl sulfate, calcium lignin sulfonate, and polyether ammonium sulfonate;

The dispersant is polyoxyethylene ether carboxylate, polyoxyethylene ether sulfonate, sodium methylene bisnaphthalene sulfonate, lignin sulfonate, bisnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate formaldehyde Condensate sodium salt, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, or phenethylphenol polyoxyethylene polyoxypropylene ether One or more of them are mixed;

The disintegrant is anhydrous ammonium sulfate, anhydrous sodium sulfate or urea;

The binder is one of vegetable gum, animal gum, rosin and its modification, cellulose and its modification, paraffin and its modification, starch and its modification, sulfur, asphalt, and gypsum. Species or a mixture of two or more species.

The invention also provides the use of the microbial bacteria-containing compound pesticide composition for controlling diseases and insect pests of rice, peanut, soybean, potato, citrus, beet, rape, pepper, cucumber, and eggplant.

Furthermore, the pesticide composition containing microbial bacteria is used for controlling lepidoptera, coleoptera, homoptera, diptera pests, underground pests and soil-borne bacterial diseases on vegetables.

The present invention mixes three insecticides, thiamethoxam, Beauveria bassiana, and Bacillus cereus, with different action mechanisms and insecticidal spectrums in a certain proportion to increase the target site of action and expand the insecticidal spectrum. The composition can well control soil-borne bacterial diseases on Lepidoptera, Coleoptera, Homoptera, Diptera pests, underground pests and vegetables. In addition, the pesticide composition containing microbial bacteria is safe for crops, and is applied to rice, peanut, soybean, potato, citrus, beet, rape, pepper, cucumber, and eggplant. It has good insecticidal effect, high efficiency and convenient use. , Low toxicity to humans and animals, environmentally friendly.

In view of this, the present invention has the following significant effects:

The active ingredients in the pesticide composition of the invention are Beauveria bassiana, Bacillus cereus, and thiamethoxam, which are low-toxic pesticides, have little environmental pollution, and are safe for phenanthrene targets such as mammals, aquatic organisms, and natural enemy insects.

The pesticide composition of the present invention is a compound preparation of thiamethoxam, Beauveria bassiana, and Bacillus cereus. Compared with a single agent component, the pesticide composition has a broad insecticidal spectrum and has both bactericidal and nematicidal effects. The number of medicines, reducing the amount of pesticides and delaying drug resistance.

detailed description

In order to make the purpose, technical solution, and advantages of the present invention more concise and clear, the present invention is illustrated by the following specific examples, but the present invention is by no means limited to these examples. The following description is only a preferred embodiment of the present invention, and is only used to describe the present invention, and should not be construed as limiting the scope of the present invention. It should be noted that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Example 1: 38% thiamethoxam · Beauveria bassiana · Bacillus cereus suspension

Mix the active ingredients and additives evenly, cut at high speed for 30 minutes in a high-speed shearing machine, and then cyclically grind in the grinder for 2-3 times to obtain a suspending agent.

Example 2: 36% thiamethoxam · Beauveria bassiana · Bacillus cereus suspension seed coating agent

The active ingredient and the auxiliary agent are mixed uniformly, and the high-speed shearing machine is sheared for 30 minutes at a high speed, and the mill is cyclically milled 2-3 times in a mill, and filtered to obtain a suspension seed coating agent.

Example 3: Wettable powder of thiamethoxam, Beauveria bassiana, and Bacillus cereus

After the active ingredients and the additives are mixed uniformly and pulverized to a certain particle size by airflow, a wettable powder can be obtained.

Example 4: 28% thiamethoxam · Beauveria bassiana · Bacillus cereus granules

The effective ingredients and additives are mixed uniformly, the mixed materials are mixed with water in a kneader, and then put into a granulator for extrusion and granulation. The extruded wet granules are dried to obtain granules.

Example 5: 58% thiamethoxam · Beauveria bassiana · Bacillus cereus water-dispersible granules

The active ingredients and additives are mixed uniformly, pulverized into a certain particle size by a jet mill, and added with an appropriate amount of water to extrude and granulate, and then dried and sieved to obtain a water-dispersible granule.

Example 6: 23% thiamethoxam · Beauveria bassiana · Bacillus cereus microemulsion

The active ingredients are fully dissolved in methyl oleate, and fatty acid polyoxyethylene phosphate, sodium alkylphenol polyoxyethylene ether sodium sulfate, glycerol, and phenylethanol oleate are mixed uniformly, and then supplemented with deionized water at high speed. After stirring and mixing, a microemulsion can be obtained.

Example 7: 32% thiamethoxam · Beauveria bassiana · Bacillus cereus water emulsion

The active ingredients, alkylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, and vegetable oil are mixed to form a uniform oil phase. Xanthan gum, ethylene glycol, silicone, and deionized water are mixed to form a uniform water phase. A water emulsion can be obtained by adding the water phase to the oil phase under high speed stirring.

Example 8: 43% thiamethoxam · Beauveria bassiana · Bacillus cereus microcapsule suspension

Dissolve the active ingredients in sodium alginate and coconut oil and stir to form a homogeneous oil phase. Add an appropriate amount to benzylphenyl polyoxyethylene ether, sodium methylene perylene naphthalene sulfonate, silicone, sodium hydroxide, and citric acid. Ionized water is mixed to form a homogeneous aqueous phase. The oil phase is added to the aqueous phase under high-speed shearing conditions, and the remainder is supplemented with deionized water to obtain a microcapsule suspension.

Example 9: Determination of indoor combined virulence of Beauveria bassiana, thiamethoxam, and Bacillus cereus to peanut root-knot nematodes.

Test worms: Pick the root-knot nematode eggs from the roots of peanuts, wash them in clean water, place them in a petri dish, and incubate in a 25 ° C ± 2 ° C incubator to obtain second-instar larvae of the same age. Water the nematodes in the petri dish Elution, filtration, centrifugation, and preparation of 200 nematode / mL suspensions with water for use.

Test method: The measurement is carried out by the immersion method, as follows.

Each pesticide treatment was set to 5 concentrations in equal proportions, and 3 mL of the medicinal solution was pipetted from a pipette into a test tube, and 3 mL of the nematode suspension was mixed until the test tube was mixed uniformly. Transfer 3mL of the mixed solution into the wells of the multi-well cell plate, cover it, and place it in a 25 ° C ± 2 ° C incubator for 24 hours. Each treatment was repeated 4 times, and a blank control was set.

Investigation method: 2 mL of the mixed solution was drawn from each treatment to observe the nematode death under a dissecting microscope, and the number of nematodes and the number of dead nematodes were recorded and investigated. Dead nematodes are stiff and cannot be moved by touching with a needle.

The co-toxicity coefficient (CTC value) of the mixture was calculated according to the Sun Yunpei method.

Found toxicity index (ATI) = (standard agents LC ₅₀ / Sample Agent LC ₅₀₎ × 100.

Theoretical virulence index (TTI) = A drug virulence index × percentage of A in the mix + B drug virulence index × percentage of B in the mix.

Co-toxicity coefficient (CTC) = ATI / TTI × 100.

According to the NY / T1154.7-2006 insecticide combined action classification criteria, CTC ≥ 120 is a synergistic effect; CTC ≤ 80 is an antagonistic effect; 80 ≤ CTC ≤ 120 is an additive effect.

Table 1. Laboratory combined virulence test results of Beauveria bassiana, thiamethoxam, and Bacillus cereus against peanut root-knot nematodes

It can be seen from Table 1 that when the Bacillus cereus, thiamethoxam, and Beauveria bassiana are compounded in a ratio range of 1 to 2: 1 to 2:15 to 25 (by mass), the co-toxicity coefficient (CTC) ) Are all greater than 120, showing synergistic effects on peanut root-knot nematodes; while in the range of 1 ～ 2: 1 ～ 2: 5, they also have an additive effect on peanut root-knot nematodes, indicating that Bacillus cereus and Thieves The combination of hydrazine and Beauveria bassiana is feasible.

Example 10: A field test of 28% thiamethoxam · Beauveria bassiana · Bacillus cereus granules (Example 4) for controlling groundnut pests in peanuts.

21% thiamethoxam · Beauveria bassiana · Bacillus cereus granules were used as test agents, 5% thiamethoxam granules, 15 billion spores / gram Beauveria bassiana granules were used as control agents, and peanuts Spray the medicine at the time of seeding and set a blank control. A pest survey was conducted 60 days after the application, and 5 sites and 50 plants were randomly investigated for each treatment, and the victim rate and control effect were calculated.

Injured plant rate (%) = number of investigated plants / total number of plants × 100

Control effect (%) = (blank control victim rate-treated victim rate) / blank control victim rate × 100

Table 2. Control effect of 28% thiamethoxam · Beauveria bassiana · Bacillus cereus granules on peanut ground pests

It can be seen from Table 2 that 15 billion spores per gram of Beauveria bassiana granules have a slightly worse control effect on peanut ground pests, only 77.53%, 28% thiamethoxam · B. Bassiana · Bacillus cereus The effect of the agent on the control of groundnut pests was better than that of 5% thiamethoxam granules, which reached 86.52%. This shows that the combined preparation has certain control effect on underground pests when applied in the field.

Example 11: A pot test of 38% thiamethoxam · Beauveria bassiana · Bacillus cereus suspension (Example 1) for controlling tomato bacterial wilt.

The soil containing the pathogen of bacterial wilt was placed in flower pots, and 15 tomato seeds were sown in each pot. After 7 days of emergence, the test agent 38% thiamethoxam · Beauveria bassiana · Bacillus cereus suspension and control agent 20 The tomato seedlings were irrigated with 100 million spores per gram of Bacillus cereus wettable powder and 20% thiasen copper suspension, and water was used as a blank control, and each treatment was set to repeat 5 times. The disease was investigated 15 days after root irrigation, and the withering rate and control effect were calculated.

Withered rate (%) = number of withered plants / total number of plants surveyed × 100

Control effect (%) = (blank treatment withered rate-treatment withered rate) / blank treatment withered rate × 100

Table 3. Control effect of thiamethoxam · Beauveria bassiana · Bacillus cereus suspension on tomato bacterial wilt

As can be seen from Table 3, compared with the control agent, the control effect of thiamethoxam · Beauveria bassiana · Bacillus cereus suspension on tomato bacterial wilt was slightly inferior to that of the control agent, but the control effect also reached 80%. The above shows that the combined preparation has certain prevention and treatment effects on bacterial diseases.

Claims (9)

1. A pesticide composition containing microbial bacteria, characterized in that it comprises the following active ingredients by weight: 1 to 2 parts of Bacillus cereus, 1 to 2 parts of thiamethoxam, and 5 to 25 parts of Beauveria bassiana .
2. The pesticide composition containing microbial bacteria according to claim 1, comprising the following active ingredients by weight: 1.2 to 1.8 parts of Bacillus cereus, 1.2 to 1.8 parts of thiamethoxam, and Beauveria bassiana 15 to 20 servings of bacteria.
3. The pesticide composition containing microbial bacteria compound according to claim 1 or 2, which is made into a liquid preparation by mixing with a solvent and an auxiliary material, characterized in that:

   The auxiliary material is one or a mixture of two or more of an emulsifier, a dispersant, a thickener, a defoamer, an antifreeze, and a pH adjuster.
4. The compound pesticide-containing pesticide composition according to claim 3, wherein:

   The solvent is methanol, ethanol, ethylene glycol, acetonitrile, toluene, xylene, acetone, N, N-dimethylformamide, dimethyl sulfoxide, epoxy soybean oil, methyl oleate, nord rosin One or more of vegetable oils;

   The emulsifiers are calcium dodecylbenzenesulfonate, sodium dodecylbenzenesulfonate, phenethylphenol polyoxyethylene ether, sorbitan monolaurate, fatty acid polyoxyethylene phosphate, One or two or more of alkylphenol polyoxyethylene ether, sodium alkylphenol polyoxyethylene ether sodium sulfate, and polysorbate are mixed;

   The dispersant is sodium methylene perylene naphthalene sulfonate, lignin sulfonate perylene naphthalene sulfonate formaldehyde condensate, polyoxyethylene ether carboxylate, alkylphenol polyoxyethylene ether phosphate, and naphthalenesulfonic acid. One or two or more of formaldehyde condensate sodium salt and polyoxyethylene ether sulfonate;

   The thickener is polyvinyl alcohol, xanthan gum, polyvinylpyrrolidone, carboxymethyl cellulose, methyl cellulose, carboxyethyl cellulose, gum arabic, sodium polyacrylate, organic bentonite, and fumed silica. Or one or two or more of magnesium aluminum silicate;

   The defoaming agent is one of polymerized glycerin, silicone, polyoxypropylene glyceryl ether, and phenylethanol oleate;

   The pH adjusting agent is one of triethanolamine, citric acid, sodium acetate, sodium hydroxide, and calcium hydroxide.
5. The pesticide composition containing microbial bacteria according to claim 1 or 2, which is formulated into a solid preparation by combining with auxiliary materials, wherein the auxiliary materials include inert fillers, wetting agents, dispersants, and disintegrating agents.
6. The pesticide composition containing microbial bacteria according to claim 5, wherein the method for preparing the solid preparation comprises the following steps:

   Mix each raw material and inert filler, pulverize into fine powder, add wetting agent, dispersant, disintegrant, mix well to make powder;

   Or on the basis of powder, add appropriate amount of binder to further prepare granules.
7. The compound pesticide-containing pesticide composition according to claim 5, characterized in that:

   The inert filler is one or a mixture of two or more of diatomite, clay, talc, high viscosity starch, kaolin, clay, bentonite, attapulgite, white carbon black, and light calcium carbonate;

   The wetting agent is sodium lauryl sulfate, sodium butylnaphthalenesulfonate, sodium alkylbenzenesulfonate, sodium fatty alcohol sulfate, sodium ligninsulfonate, sodium methylenebisnaphthalenesulfonate, and lauryl alcohol. One or two or more of sodium ether sulfate, sodium fatty acid polyoxyethylene ether sulfonate, calcium dodecyl sulfate, calcium lignin sulfonate, and polyether ammonium sulfonate;

   The dispersant is polyoxyethylene ether carboxylate, polyoxyethylene ether sulfonate, sodium methylene bisnaphthalene sulfonate, lignin sulfonate, bisnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate formaldehyde Condensate sodium salt, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, or phenethylphenol polyoxyethylene polyoxypropylene ether One or more of them are mixed;

   The disintegrant is anhydrous ammonium sulfate, anhydrous sodium sulfate or urea;

   The binder is one of vegetable gum, animal gum, rosin and its modification, cellulose and its modification, paraffin and its modification, starch and its modification, sulfur, asphalt, and gypsum. Species or a mixture of two or more species.
8. The use of the microbial bacteria-containing pesticide composition according to any one of claims 1 to 7 for controlling pests and diseases of rice, peanut, soybean, potato, citrus, beet, rapeseed, pepper, cucumber, and eggplant.
9. The pesticidal composition containing a microbial flora according to any one of claims 1 to 7 for controlling soil-borne bacterial properties on Lepidoptera, Coleoptera, Homoptera, Diptera pests, underground pests and vegetables Use of disease.