CN115011525A - Compound microbial agent for inhibiting tomato bacterial wilt and preparation method of reinforced compost - Google Patents

Compound microbial agent for inhibiting tomato bacterial wilt and preparation method of reinforced compost Download PDF

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CN115011525A
CN115011525A CN202210741977.3A CN202210741977A CN115011525A CN 115011525 A CN115011525 A CN 115011525A CN 202210741977 A CN202210741977 A CN 202210741977A CN 115011525 A CN115011525 A CN 115011525A
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丁国春
李蕙秀
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China Agricultural University
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Abstract

The invention relates to the technical field of microbial fertilizers, and provides a compound microbial bacterium for inhibiting tomato bacterial wiltThe preparation method of the agent and the reinforced compost comprises a composite microorganism and a carrier, wherein the composite microorganism comprises Bacillus methylotrophicus BOX10 and Bacillus cereus BOX 11; the concentration of each bacterium in the microbial inoculum is 1.0 multiplied by 10 9 ～5.0×10 9 CFU/g. The compound microbial agent disclosed by the invention can effectively reduce the incidence rate of bacterial wilt, and the control effect can be improved by 60-73%. The compound microbial agent is applied to soil as a bacterial fertilizer, so that the soil and rhizosphere microbial environment can be improved, the tomato bacterial wilt can be inhibited, and the plant growth can be promoted.

Description

Compound microbial agent for inhibiting tomato bacterial wilt and preparation method of reinforced compost
Technical Field
The invention relates to the technical field of microbial fertilizers, in particular to a compound microbial agent for inhibiting tomato bacterial wilt and a preparation method of reinforced compost.
Background
Bacterial wilt is widely distributed in tropical or subtropical regions and can damage more than 50 families and more than 200 plants, such as important commercial crops of tomatoes, eggplants, peanuts, bananas, tobaccos and the like. Ralstonia solanacearum (Ralstonia solanacearum) causing bacterial wilt can enter a pseudo-death state in soil for long-term survival. More than 30 provinces in China have bacterial wilt which is reported to cause serious disease in southern areas. With global warming and greenhouse planting popularization, the hazard degree of bacterial wilt is continuously increased, and the disease incidence range tends to gradually move to the north. Efficient chemical agents are not available in the aspect of preventing and controlling bacterial wilt, the modes of soil fumigation and the like are expensive, and farmers often adopt the mode of abandoning host crops in diseased land to prevent and control the bacterial wilt. Therefore, it is of great significance to seek a green, efficient, environmentally friendly and feasible strategy to prevent or inhibit bacterial wilt.
Aerobic fermentation can easily realize organic waste treatment and resource conversion, and the fermentation product compost often has an inhibiting effect on various plant diseases, such as fungi (fusarium wilt, verticillium wilt, saprophytic disease, rhizoctonia disease and the like) and bacteria (bacterial wilt, macula disease, leaf blight) diseases. The function of microorganisms in compost for controlling plant diseases has been studied, but no clear conclusion is made. Some of the researches support that compost microorganisms are key factors for inhibiting plant diseases, for example, a large amount of microorganisms having antagonistic action on plant pathogens, such as Pseudomonas and Bacillus, exist in compost, and the disease inhibiting action of the compost after the sterilization treatment is reduced or even completely eliminated; on the other hand, modern omics technology shows that the microbiome in compost is greatly different from the microbial population of soil and rhizosphere, and the abundance of the main group in compost is rapidly reduced after compost application, and these results show that the main group in compost cannot survive in soil in a large amount. Our investigations of the national microbiome of compost have also shown that the core microbiome in compost is mainly involved in the degradation of organic waste, and does not contain microorganisms involved in the inhibition of plant diseases. The analysis results of 38 kinds of composts collected by 38 composting plants in 6 provinces of China are also displayed: the compost has unstable effect on preventing and treating the tomato bacterial wilt, 72.7 percent of the compost has the effect of inhibiting the disease, and 27.3 percent of the compost can promote the disease. This result indicates that the effect of different composts on bacterial wilt inhibition is unstable. Rhizosphere microorganisms with disease inhibiting function, such as multiple species of bacillus, have important function in the aspect of plant health protection, and the effect of inhibiting the tomato bacterial wilt of the disease-resistant microorganism strengthened compost under different ecological conditions can be further increased by compounding the multiple species.
Disclosure of Invention
The invention aims to provide a compound microbial agent for inhibiting tomato bacterial wilt and a preparation method of reinforced compost.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a compound microbial agent for inhibiting tomato bacterial wilt, which comprises compound microorganisms and a carrier, wherein the compound microorganisms comprise bacillus methylotrophicus BOX10 and bacillus cereus BOX 11; the concentration of each bacterium in the microbial inoculum is 1.0 multiplied by 10 9 ～5.0×10 9 CFU/g。
Further, the carrier is diatomite.
The invention also provides a preparation method of the compound microbial agent, which comprises the following steps:
(1) separately carrying out fermentation culture on bacillus methylotrophicus and bacillus cereus to obtain a zymophyte liquid, and mixing;
(2) and (2) mixing the zymophyte liquid obtained in the step (1) with a carrier, and drying to obtain the dry microbial inoculum powder.
Further, the culture medium for fermentation culture in the step (1) comprises the following components in parts by weight: 60-70 parts of bran, 25-35 parts of soybean meal, 1-4 parts of corn pulp powder and KH 2 PO 4 0.1 to 0.5 portion of MgSO 4 0.2 to 1 part of MnSO 4 0.01 to 0.05 part of CaCO 3 3-6 parts of water, 180-220 parts of water, and the pH value of the fermentation medium is 6.8-7.3.
Further, the ratio of the zymocyte liquid to the carrier in the step (2) is 1-2 mL: 1-2 g.
Further, the drying temperature in the step (2) is 55-65 ℃, and the drying time is 24-36 hours; the water content of the microbial inoculum dry powder is less than 10%.
The invention also provides a method for preparing bacterial fertilizer by fermenting the compound microbial agent, which comprises the following steps: adjusting the carbon nitrogen ratio of the organic waste to be 25-30, adjusting the water content of the organic waste to be 55-65%, fermenting for 7-12 days, cooling to 38-45 ℃, adding the composite microbial agent, fermenting for 2-3 days, and aging to obtain the bacterial fertilizer.
Further, the organic waste is one or more of mushroom residue, livestock and poultry manure and straw.
Further, an intermittent aeration method is adopted for aeration in the fermentation process, the aeration time is 10-20 min/h, and the aeration intensity is 10-100 L.L -1 ·min -1 。
Further, the volume ratio of the compound microbial agent to the organic waste is 1-3: 100.
Compared with the prior art, the invention has the beneficial effects that:
the compound microbial agent for preventing and treating tomato bacterial wilt comprises bacillus methylotrophicus BOX10 and bacillus cereus BOX11, and greenhouse potting experiments show that: under the challenge inoculation of high-concentration ralstonia solanacearum, the morbidity of a control group is 85% and 92%, the morbidity of a compound microbial agent treatment group is reduced by 48% and 57%, and the control effect is 56% and 62%, so that the results show that the compound microbial agent can obviously inhibit the ralstonia solanacearum (P)<0.05). Rhizosphere microbiome experiments show that the relative abundance of the inoculated compound microbial agent in the root system can be 0.5-1.7%. The abundance of two antagonistic bacteria (BOX10, BOX11) in the compost formed by the bacterial manure integrated fermentation process exceeds 10 9 CFU/g. Greenhouse experiments show that: high concentration withered seed pairThe morbidity of the control group exceeds 82%, the morbidity of the compost treatment group formed by the integrated fermentation process does not exceed 32%, and the control effect is improved by 60-73%. The analysis of rhizosphere microbiome also shows that the abundance of bacillus and flavobacterium in tomato rhizosphere is obviously improved under the composting treatment, and the two groups contain a large amount of bacterial wilt resisting bacteria.
Drawings
FIG. 1 is a graph showing the inhibition of the incidence of tomato bacterial wilt by the addition of a complex microbial inoculant;
FIG. 2 is a graph showing the incidence of bacterial wilt inhibition by bacterial manure added with compound microbial inoculum;
FIG. 3 is a diagram showing the analysis of the co-occurrence network of rhizosphere microbiome.
Detailed Description
The invention provides a compound microbial agent for inhibiting tomato bacterial wilt, which comprises compound microorganisms and a carrier, wherein the compound microorganisms comprise bacillus methylotrophicus BOX10 and bacillus cereus BOX 11.
In the present invention, the Bacillus methylotrophicus BOX10 and Bacillus cereus BOX11 are referred to BOX10 and BOX11 in "Long-Term Organic farm manufactured microbial and Bacillus Antagonism Against scraper (Phytophthora capsicii)".
In the invention, the concentration of each bacterium in the microbial inoculum is 1.0 multiplied by 10 9 ～5.0×10 9 CFU/g, preferably 2.0X 10 9 ～4.0×10 9 CFU/g, more preferably 2.5X 10 9 ～3.5×10 9 CFU/g。
In the present invention, the carrier is preferably diatomaceous earth.
The invention also provides a preparation method of the compound microbial agent, which comprises the following steps:
(1) separately carrying out fermentation culture on bacillus methylotrophicus and bacillus cereus to obtain a zymophyte liquid, and mixing;
(2) and (2) mixing the zymophyte liquid obtained in the step (1) with a carrier, and drying to obtain the dry microbial inoculum powder.
In the present invention, Bacillus methylotrophicus is usedCarrying out fermentation culture on the bacillus cereus to obtain a zymophyte liquid; the culture medium for fermentation culture comprises the following components in parts by weight: 60-70 parts of bran, 25-35 parts of soybean meal, 1-4 parts of corn pulp powder and KH 2 PO 4 0.1 to 0.5 portion of MgSO 4 0.2 to 1 part of MnSO 4 0.01 to 0.05 part of CaCO 3 3-6 parts of water, 180-220 parts of water, and the pH value of the fermentation medium is 6.8-7.3.
In the invention, the culture medium for preparing the fermentation culture comprises 60-70 parts by weight of bran, preferably 62-68 parts by weight of bran, and further preferably 64-66 parts by weight of bran.
In the invention, the culture medium for preparing the fermentation culture comprises 25-35 parts by weight of soybean meal, preferably 27-33 parts by weight of soybean meal, and further preferably 29-31 parts by weight of soybean meal.
In the invention, the culture medium for preparing the fermentation culture comprises 1-4 parts by weight of corn steep liquor powder, preferably 1.5-3.5 parts by weight, and further preferably 2-3 parts by weight.
In the invention, the culture medium for preparing the fermentation culture comprises KH in parts by weight 2 PO 4 0.1 to 0.5 part, preferably 0.2 to 0.4 part, and more preferably 0.25 to 0.35 part.
In the invention, the culture medium for preparing the fermentation culture comprises MgSO (MgSO) 4 0.2 to 1 part, preferably 0.3 to 0.8 part, and more preferably 0.5 to 0.6 part.
In the invention, the culture medium for preparing the fermentation culture comprises MnSO (manganese-SO) in parts by weight 4 0.01 to 0.05 part, preferably 0.02 to 0.04 part, and more preferably 0.025 to 0.035 part.
In the invention, the culture medium for preparing the fermentation culture comprises CaCO in parts by weight 3 3 to 6 parts, preferably 4 to 5 parts, and more preferably 4.4 to 4.6 parts.
In the invention, the culture medium for preparing the fermentation culture comprises 180-220 parts by weight of water, preferably 190-210 parts by weight of water, and more preferably 198-205 parts by weight of water.
In the invention, the zymocyte liquid obtained in the step (1) is mixed with a carrier, and the mixture is dried to obtain dry microbial inoculum powder; the ratio of the zymocyte liquid to the carrier is preferably 1-2 mL: 1-2 g, and more preferably 1mL:1 g.
In the invention, the drying temperature is 55-65 ℃, preferably 56-64 ℃, and more preferably 58-62 ℃; the drying time is 24-36 h, preferably 26-34 h, and further preferably 28-32 h.
In the invention, the moisture content of the prepared microbial inoculum dry powder is less than 10%, preferably 2-8%, and more preferably 5-7%.
The invention also provides a method for preparing bacterial fertilizer by fermenting the compound microbial agent, which comprises the following steps: adjusting the carbon-nitrogen ratio of the organic waste, adjusting the water content of the organic waste for fermentation, adding the compound microbial agent when the temperature is reduced to 38-45 ℃ for 7-12 days of fermentation, fermenting for 2-3 days, and aging to obtain the bacterial fertilizer.
In the invention, the carbon-nitrogen ratio of the organic waste is 25-30, preferably 26-29, and more preferably 27-28; the method for adjusting the carbon-nitrogen ratio specifically comprises the steps of selecting fermentation raw materials with different carbon-nitrogen ratios and mixing the fermentation raw materials according to the required carbon-nitrogen ratio.
In the present invention, the water content of the organic waste is 55 to 65%, preferably 57 to 63%, and more preferably 59 to 61%.
In the invention, the organic waste is one or more of mushroom residues, livestock and poultry manure and straws, and preferably mushroom residues.
In the invention, an intermittent aeration method is adopted for aeration in the fermentation process, and the aeration time is 10-20 min/h, preferably 12-18 min/h, and further preferably 14-16 min/h; the aeration intensity is 10-100 L.L -1 ·min -1 Preferably 20 to 80 L.L -1 ·min -1 More preferably 40 to 60 L.L -1 ·min -1 。
In the invention, the volume ratio of the compound microbial agent to the organic waste is 1-3: 100, preferably 1.5-2.5: 100, and further preferably 1.8-2.2: 100.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The embodiment provides a compound microbial agent for inhibiting tomato bacterial wilt, which comprises compound microorganisms and diatomite, wherein the compound microorganisms comprise bacillus methylotrophicus BOX10 and bacillus cereus BOX11, and the concentration of each bacterium is 1.0 multiplied by 10 respectively 9 CFU/g。
The preparation method of the compound microbial agent comprises the following steps:
(1) carrying out single fermentation culture on bacillus methylotrophicus and bacillus cereus to obtain a zymocyte liquid, and then mixing, wherein a culture medium for fermentation culture comprises: 65g of bran, 30g of soybean meal, 2g of corn milk powder and KH 2 PO 4 0.2g，MgSO 4 0.5g，MnSO 4 0.02g，CaCO 3 4.5g, the ratio of material to water is 1:2, and the PH value is adjusted to 7 by lime;
(2) and (2) mixing the zymocyte liquid obtained in the step (1) with diatomite according to a ratio of 1mL to 1g, and drying at 55 ℃ for 36h to obtain a microbial inoculum dry powder, wherein the water content of the microbial inoculum dry powder is 8%.
The method for preparing the bacterial fertilizer by fermenting the prepared compound microbial agent comprises the following steps: selecting mushroom residue as fermentation substrate, adjusting carbon nitrogen ratio of mushroom residue to 25, water content to 60%, fermenting, and aerating by intermittent aeration method for 15min/h with aeration intensity of 50 L.L -1 ·min -1 And when the temperature is reduced to 40 ℃ after fermentation for 10 days, adding the compound microbial agent, wherein the volume ratio of the compound microbial agent to the organic waste is 1: 100, fermenting for 3 days, and aging to obtain the bacterial fertilizer.
Example 2
The embodiment provides a compound microbial agent for inhibiting tomato bacterial wilt, which comprises compound microorganisms and diatomite, wherein the compound microorganisms comprise bacillus methylotrophicus BOX10 and bacillus cereus BOX11, and the concentration of each bacterium is 3.0 multiplied by 10 9 CFU/g。
The preparation method of the compound microbial agent comprises the following steps:
(1) carrying out fermentation culture on bacillus methylotrophicus and bacillus cereus to obtain a zymocyte liquid, wherein a culture medium for the fermentation culture comprises: 60g of bran, 35g of soybean meal, 1g of corn milk powder and KH 2 PO 4 0.4g，MgSO 4 0.2g，MnSO 4 0.05g，CaCO 3 5g, the material-water ratio is 1:2, and the PH value is adjusted to 7.2 by lime;
(2) and (2) mixing the zymocyte liquid obtained in the step (1) with diatomite according to a ratio of 1mL to 2g, and drying at 60 ℃ for 30h to obtain a microbial inoculum dry powder, wherein the water content of the microbial inoculum dry powder is 9%.
The method for preparing the bacterial fertilizer by fermenting the prepared compound microbial agent comprises the following steps: selecting livestock and fowl feces as fermentation substrate, adjusting carbon-nitrogen ratio of mushroom residue to 30, water content to 62%, fermenting, and aerating by intermittent aeration method for 20min/h with aeration intensity of 80 L.L -1 ·min -1 And when the temperature is reduced to 38 ℃ after 12 days of fermentation, adding the compound microbial agent, wherein the volume ratio of the compound microbial agent to the organic waste is 2: 100, fermenting for 3 days, and aging to obtain the bacterial fertilizer.
Example 3
The embodiment provides a compound microbial agent for inhibiting tomato bacterial wilt, which comprises compound microorganisms and diatomite, wherein the compound microorganisms comprise bacillus methylotrophicus BOX10 and bacillus cereus BOX11, and the concentration of each bacterium is 3.0 multiplied by 10 respectively 9 CFU/g。
The preparation method of the compound microbial agent comprises the following steps:
(1) carrying out fermentation culture on bacillus methylotrophicus and bacillus cereus to obtain a zymocyte liquid, wherein a culture medium for the fermentation culture comprises: 70g of bran, 25g of soybean meal, 4g of corn milk powder and KH 2 PO 4 0.3g，MgSO 4 0.2g，MnSO 4 0.03g，CaCO 3 4g, the ratio of material to water is 1:2, and the PH value is adjusted to 6.9 by lime;
(2) and (2) mixing the zymocyte liquid obtained in the step (1) with diatomite according to a ratio of 2mL to 1g, and drying at 65 ℃ for 24 hours to obtain a microbial inoculum dry powder, wherein the water content of the microbial inoculum dry powder is 7%.
The method for preparing the bacterial fertilizer by fermenting the prepared compound microbial agent comprises the following steps: selecting mushroom residue as fermentation substrate, adjusting carbon-nitrogen ratio of mushroom residue to 28, water content to 58%, fermenting, and aerating by intermittent aeration method for 12min/h with aeration intensity of 40 L.L -1 ·min -1 And when the temperature is reduced to 42 ℃ after 9 days of fermentation, adding a compound microbial agent, wherein the volume ratio of the compound microbial agent to the organic waste is 3: 100, fermenting for 3 days, and aging to obtain the bacterial fertilizer.
Examples of the experiments
Experiments were conducted using the complex microbial inoculant and the fermented bacterial manure prepared in example 1.
1. Preparation of ralstonia solanacearum inoculation liquid
The bacterial strain of ralstonia solanacearum, which was gift from Jiangchunhaobo university at Nanjing university of agriculture, was activated with YGPA medium (Yeast extract 5g, peptone 5g, anhydrous glucose 10g, agar 15g, 1L deionized water, sterilized at 121 ℃ for 20 min). After culturing at 28 ℃ for 2d, selecting single bacterium, streaking, and culturing at 28 ℃ for 2 d. The cells were eluted with 10mL sterile water, and then subjected to gradient dilution and OD 600 Measurement of light absorption value, plotting OD 600 And the linear relation with the corresponding CFU is used as a basis for estimating the concentration of the bacterial suspension prepared subsequently.
2. Compound bacterium agent effect verification
The tomato culture medium is a mixture of clean sterilized sea sand (sterilization conditions: 121 ℃, 20min) and soil, and the mixing ratio is 5: 1. after pregermination, the healthy tomato seeds were sown in pots containing 600g of the above soil-sand mixture, 6 seedlings per pot. Placing into a light incubator (light intensity 15000Lx, light period 16h/8h) according to the principle of random complete block, culturing at 30 deg.C under 95% relative humidity, and culturing with Hoagland nutrient solution as nutrient solution. Randomly and evenly dividing tomato seedlings into 2 groups, inoculating a compound microbial agent to an experimental group (MC), inoculating a compound microbial agent to a blank control group (CK), culturing 21d of the experimental group to inoculate the compound microbial agent, and mixing the compound microbial agent with sterile water according to the ratio of 1: 9(w/v) dilution to form a suspensionRoot irrigation, 6 ml/basin, CK group inoculation of equal volume of sterile water. After 28 days of culture, the bacterial liquid of freshly cultured ralstonia solanacearum is inoculated, and the concentration is 10 8 CFU/mL, applied at 6 mL/bowl. And (4) counting the incidence rate after inoculating the pathogenic bacteria for 14 d. As shown in FIG. 1, it can be seen from FIG. 1 that the incidence of tomato seedlings in the experimental group MC is significantly lower than that in the control group CK (p)<0.05)。
3. Effect verification of composite microbial inoculum reinforced compost
In the greenhouse experiment, tomato seedlings are randomly and averagely divided into 2 groups, the group added with the fermentation bacterial manure of the example 1 is an experimental group (MCC), the experimental group performs two repeated experiments (MCC1 and MCC2), and the group not added is a blank control group (CK). The experimental group mixed the fermented bacterial manure into the soil according to the proportion of 3%. Transplanting healthy tomato seedlings in 4-leaf stage into experimental group pots and blank control group pots, and continuously inoculating for 4d after seedling transplanting by 10 8 The bacterial wilt in CFU/mL is treated for 3 times, and each seedling is treated for 1 mL/time. And culturing the treated tomato seedlings under the conditions that the temperature is 30 ℃, the relative humidity is 90% and the photoperiod is 16h/8h, and counting the disease occurrence condition of the tomato seedlings after inoculation is completed for 3 d. The results are shown in FIG. 2, CK: blank control; MCC 1: enhanced compost 1, MCC 2: the intensified compost 2, as can be seen from fig. 2, the tomato seedlings added with the fermented bacterial manure have a significantly lower incidence than the control group (p)<0.05)。
4. Rhizosphere microbiome diversity study
The healthy tomato seeds after pregermination were sown in soil containing 600g of 3% fermented bacterial manure, 6 seedlings per pot. Soil without zymophyte was used as Control (CK). Placing into a light incubator (light intensity 15000Lx, light period 16h/8h) according to the random complete block principle, and culturing at 30 deg.C under 95% relative humidity for 28 d. Rhizosphere samples were collected, each containing 6 seedlings, and 4 samples were processed each. The rhizosphere microorganism diversity analysis method comprises the following steps: TC-DNA was extracted using the FastDNA Spin Kit for Soil (MP, Biomedicals, Santa Ana, Carlsbad, CA, United States) Kit. The primers used for 16S rRNA gene amplification are:
515F(SEQ ID NO:1)：5’-GTGCCAGCMGCCGCGGTAA-3’；
909R(SEQ ID NO:2)：5’-CCCCGYCAATTCMTTTRAGT-3’。
16S rRNA analysis the analytical tool on freebieoinfo website (www.freebioinfo.org) was used. The results are shown in fig. 3, and it can be seen from fig. 3 that the number of rhizosphere bacillus modules of tomato administered with the disease-resistant compost is significantly increased.
According to the embodiments, the invention provides the compound microbial agent for inhibiting the tomato bacterial wilt and the preparation method of the enhanced compost.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.
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Claims (10)

1. Tomato bacterial wilt inhibition methodThe compound microbial agent is characterized by comprising a compound microorganism and a carrier, wherein the compound microorganism comprises Bacillus methylotrophicus BOX10 and Bacillus cereus BOX 11; the concentration of each bacterium in the microbial inoculum is 1.0 multiplied by 10 9 ～5.0×10 9 CFU/g。
2. The complex microbial inoculant according to claim 1, wherein said carrier is diatomaceous earth.
3. The method for preparing the complex microbial inoculant of claim 1 or 2, which comprises the following steps:
(1) separately carrying out fermentation culture on bacillus methylotrophicus and bacillus cereus to obtain a zymophyte liquid, and mixing;
(2) and (2) mixing the zymophyte liquid obtained in the step (1) with a carrier, and drying to obtain the dry microbial inoculum powder.
4. The method for preparing a complex microbial inoculant according to claim 3, wherein the culture medium for fermentation culture in step (1) comprises the following components in parts by weight: 60-70 parts of bran, 25-35 parts of soybean meal, 1-4 parts of corn pulp powder and KH 2 PO 4 0.1 to 0.5 portion of MgSO 4 0.2 to 1 part of MnSO 4 0.01 to 0.05 part of CaCO 3 3-6 parts of water, 180-220 parts of water, and the pH value of the fermentation medium is 6.8-7.3.
5. The method for preparing a composite microbial inoculant according to claim 3, wherein the ratio of the zymocyte liquid to the carrier in the step (2) is 1-2 mL: 1-2 g.
6. The preparation method of the compound microbial agent according to claim 3, wherein the drying temperature in the step (2) is 55-65 ℃, and the drying time is 24-36 h; the water content of the microbial inoculum dry powder is less than 10%.
7. The method for preparing bacterial fertilizer by fermenting the compound microbial agent as claimed in claim 1, which is characterized by comprising the following steps: adjusting the carbon-nitrogen ratio of the organic waste to be 25-30, adjusting the water content of the organic waste to be 55-65%, fermenting for 7-12 days, cooling to 38-45 ℃, adding the compound microbial agent, fermenting for 2-3 days, and aging to obtain the bacterial fertilizer.
8. The method as claimed in claim 7, wherein the organic waste is one or more of mushroom residue, livestock and poultry manure, and straw.
9. The method according to claim 7, wherein the fermentation process is aerated by intermittent aeration, the aeration time is 10-20 min/h, and the aeration intensity is 10-100L-L -1 ·min -1 。
10. The method according to claim 7, wherein the volume ratio of the composite microbial agent to the organic waste is 1-3: 100.
CN202210741977.3A 2022-06-28 2022-06-28 Compound microbial agent for inhibiting tomato bacterial wilt and preparation method of reinforced compost Pending CN115011525A (en)

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