WO2020049228A1 - Utilisation de rosine pour le traitement de semences - Google Patents

Utilisation de rosine pour le traitement de semences Download PDF

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Publication number
WO2020049228A1
WO2020049228A1 PCT/FI2019/050644 FI2019050644W WO2020049228A1 WO 2020049228 A1 WO2020049228 A1 WO 2020049228A1 FI 2019050644 W FI2019050644 W FI 2019050644W WO 2020049228 A1 WO2020049228 A1 WO 2020049228A1
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WIPO (PCT)
Prior art keywords
rosin
composition
seeds
fungi
acid
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PCT/FI2019/050644
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English (en)
Inventor
Jani Korhonen
Tero HÄNNINEN
Seppo Hänninen
Original Assignee
Woimet Oy
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Publication date
Application filed by Woimet Oy filed Critical Woimet Oy
Priority to EP19773134.2A priority Critical patent/EP3846622A1/fr
Priority to CA3111659A priority patent/CA3111659A1/fr
Priority to AU2019335556A priority patent/AU2019335556A1/en
Publication of WO2020049228A1 publication Critical patent/WO2020049228A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents

Definitions

  • the present disclosure relates generally to compositions for protecting plants from pathogens; and more specifically, to use of compositions comprising rosin and at least one solvent for seed treatment. Furthermore, the present disclosure also relates to methods of treating seeds using the aforementioned compositions.
  • chemical pesticides and biocides are associated with major drawbacks.
  • chemical pesticides are toxic to both organisms that they are intended therefor such as the pathogens and/or microbes, and also to other organisms such as cattle, stray animals, pets and even humans.
  • certain chemical pesticides are often effective for only a short period on particular organisms. In such instances, the organisms become immune to the chemical pesticides after prolonged exposure thereto and consequently, the chemical pesticides may no longer be effective in preventing growth and harm to the crops by the organisms.
  • the chemical pesticides may lead to bio-magnification, wherein chemical residues of the chemical pesticides left behind in the crops or plants, makes them unfit for human or animal consumption.
  • the chemical pesticides may soak into soil in the crop-fields and/or underground water used for irrigation in the crop-fields, thereby contaminating the soil and/or underground water with the chemical residues. It will be appreciated that when such contaminated soil is exposed to animals and/or humans, the contaminated water is used to spray the crops, or used for animal or human consumption, the animals and/or humans may be at great risk of experiencing severe damage including brain damage, cancer or even death.
  • seed treatment or seed dressing is a compound, typically antimicrobial or fungicidal, that is used for treating (or "dressing") seeds prior to planting thereof.
  • seed treatment employs comparatively smaller quantities of chemical pesticides than spraying of crop-fields, the technique still poses various risks.
  • One such risk is accidental exposure of workers performing the seed treatment to the chemicals used for the seed treatment, wherein such chemicals can be harmful to the workers.
  • Another major risk is causing harm to the sensitive seeds by exposure thereof to the chemicals, thereby, leading to unsuccessful growth (or premature death) of crops.
  • food supplies resulting from the plants or crops grown from the treated seeds may be contaminated by the chemicals. It will be appreciated that consumption of such food supplies can be detrimental to health and well-being of humans and/or animals consuming the food supplies.
  • CN106259564 A discloses a coating film for corn seeds, wherein the coating film aims at keeping the normal water content required for seed germination and for preventing seeds from being inactivated due to water shortage.
  • EA027877 B1 relates to prevention of rapeseed pod from cracking by forming a coating about the pod.
  • Arruda et al (2007) studied the possible phytotoxic effects of some natural and synthetic substances potentially repellent to wild fauna.
  • these references do not relate to antimicrobial or antifungal seed treatment or seed dressing.
  • the present disclosure seeks to provide a use of a composition comprising rosin and at least one solvent for seed treatment.
  • the present disclosure also seeks to provide a method of treating seeds using the aforementioned composition.
  • the present disclosure seeks to provide a solution to the existing problem of protecting plants from microbial pathogens without causing harm to the plant or the environment.
  • An aim of the present disclosure is to provide a solution that overcomes at least partially the problems encountered in prior art.
  • an embodiment of the present disclosure provides a use of a composition comprising rosin and at least one solvent for seed treatment.
  • an embodiment of the present disclosure provides a method of treating seeds, comprising:
  • Embodiments of the present disclosure substantially eliminate or at least partially address the aforementioned problems in the prior art, and provide the composition that is effective in concentrations significantly smaller as compared to conventional products for its use in treating seeds for protecting seeds from pathogens and microbes in the environment. Additional aspects, advantages, features and objects of the present disclosure would be made apparent from the drawings and the detailed description of the illustrative embodiments construed in conjunction with the appended claims that follow.
  • FIGs. 1A to 1C are graphical representations of average growth of fungi that is grown for a predefined period of time, in different concentrations of composition comprising rosin and at least one solvent, under dark conditions, in accordance with an embodiment of the present disclosure
  • FIGs. 2A to 2E are graphical representations of average growth of fungi that is grown for a predefined period of time, in different concentrations of composition comprising rosin and at least one solvent, exposed to near visible ultraviolet (NUV) light, in accordance with another embodiment of the present disclosure.
  • NUV near visible ultraviolet
  • FIG. 3 illustrates steps of a method of treating seeds, in accordance with a further embodiment of the present disclosure.
  • FIG. 5 illustrates results obtained in preventing formation of heads with smut in oats in field trials when oat seeds were treated with different concentrations of composition comprising rosin and water as solvent before sowing.
  • an underlined number is employed to represent an item over which the underlined number is positioned or an item to which the underlined number is adjacent.
  • a non-underlined number relates to an item identified by a line linking the non-underlined number to the item. When a number is non-underlined and accompanied by an associated arrow, the non-underlined number is used to identify a general item at which the arrow is pointing.
  • an embodiment of the present disclosure provides a use of a composition comprising rosin and at least one solvent for seed treatment.
  • a method of treating seeds comprising:
  • the present disclosure provides the aforementioned use of the composition for seed treatment and the aforementioned method of treating seeds using such composition.
  • the composition comprises rosin, which is a wood-based bioactive ingredient possessing antimicrobial, antibacterial, antifungal and antiviral properties.
  • the rosin is readily soluble in solvents, such as an organic solvent. Notably, such solvents are highly volatile and evaporate with relative ease, resulting in formation of a protective layer of rosin around the seeds treated with the composition comprising rosin and at least one solvent.
  • solvents are highly volatile and evaporate with relative ease, resulting in formation of a protective layer of rosin around the seeds treated with the composition comprising rosin and at least one solvent.
  • rosin is less harmful as compared to commercially- available pesticides and therefore, is suitable for use for protecting plants, specifically at the seed germination level, without leading to any unpredictable environmental interactions.
  • a small concentration of rosin is already effective in preventing the growth of fungi, as compared to substantially high concentrations of
  • plants are subjected to growth of fungus thereon, such as, during exposure to environmental conditions during various stages of plant growth, ranging from seed germination to fruit-formation.
  • fungus refers to a group of eukaryotic organisms that include microorganisms such as yeast, moulds and mushrooms.
  • Fungi are the most widely distributed organisms on earth and exist in either free-form in soil, air and water or in symbiotic relationships with other organisms, such as plants, animals, parasites, other fungi and humans, for their nutritional needs. Fungi have long been used as a source of human food and in processing thereof.
  • fungi have been employed in production of antibiotics and various industrial applications, such as detergents. Despite the benefits, some bioactive compounds produced by fungi are toxic to plants, animals and humans.
  • the plants inherently produce antifungal compounds, such as resins, gums, phytotoxins, polyphenols, saponins, fatty acids, essential oils, flavonoids, peptides and so forth, to resist the fungal infections.
  • antifungal compounds such as resins, gums, phytotoxins, polyphenols, saponins, fatty acids, essential oils, flavonoids, peptides and so forth.
  • composition comprising rosin and the at least one solvent for seed treatment.
  • composition refers to a plant growth regulating agent that is applied externally to the plant surface, such as by spraying, dusting or dipping the plant therein and so forth.
  • plants refers to a plant in whole or in parts, such as seeds, root, stem, flowers, leaves and so forth.
  • rosin refers to a solid form of a plant-based ingredient 'resin' obtained, generally, from coniferous trees and pines. Rosin possesses antimicrobial, antibacterial, antifungal and antiviral properties and can be used in protecting plants from various bacteria, viruses and fungi present in the environment surrounding the plant treated with the rosin. It will be appreciated that naturally existing rosin comprises various bioactive ingredients, such as acids and other derivatives. Typically, rosin exists in nature in either a solid form or free form. Optionally, the rosin is in a form of free rosin acid or in a form of its alkali metal, earth alkali metal, ammonium or amine salt.
  • the free rosin acid comprise largely abietic acid, pyroabietic acid, dehydroabietic acid, 7a-hydroxydehydroabietic acid, 7b- hydroxydehydroabietic acid, 15-hydroxydehydroabietic acid, 7a, 15- hydroxydehydroabietic acid, 73,15-dihydroxydehydroabietic acid, 18- hydroxydehydroabietic acid, pimaric acid, sapinic acid and the like rosin acids and/or mixtures thereof, and the alkali metal salt of rosin comprise for example a sodium salt of rosin, potassium salt of rosin, and the like.
  • commercially available form of rosin i.e.
  • tall oil rosin comprises rosin in the form of at least one of the free rosin acid or the sodium salt of rosin. More specifically, the biological activity of the free rosin acid and the sodium salt of rosin are typically identical. However, the sodium salt of rosin is soluble in water while the free rosin acid in solid form is insoluble in water. Furthermore, the insolubility of solid form of rosin in water contemplates the use of a solvent, such as an organic solvent, including but not limited to, an acetic acid, an alcohol, turpentine, acetone, ethyl acetate, and an ether, that dissolves the rosin. As mentioned earlier, the solvent may also be water.
  • a solvent such as an organic solvent, including but not limited to, an acetic acid, an alcohol, turpentine, acetone, ethyl acetate, and an ether, that dissolves the rosin.
  • the solvent may also be water.
  • the composition may comprise a commercially available rosin, such as the tall oil rosin (CAS-number 8050-09-7), and at least one alcohol.
  • the composition may also comprise (in addition or alternatively) an ammonium or amine salt of rosin.
  • the composition comprises a combination of the free rosin acid and the alkali metal salt of rosin and at least one solvent, such as aqueous alcohol, particularly in such amounts that both the free rosin acid and the alkali metal salt or rosin are soluble.
  • solvent such as aqueous alcohol
  • the use of the composition comprising rosin and at least one solvent for seed treatment requires subjecting the seeds to such composition.
  • the composition comprising both forms of rosin and at least one solvent when applied to the plant results in a dual benefit to the plant.
  • the composition comprising rosin and the at least one solvent when used for seed treatment, the at least one solvent evaporates over a period of time leaving behind a protective coating of free rosin acid around the plant, while the alkali salt of rosin, such as for example a sodium salt of rosin, provides its antimicrobial effect also to the environment surrounding the plant, such as the soil around the seed or growth medium in a hydroponics arrangement for growing plants.
  • the alkali salt of rosin such as for example a sodium salt of rosin
  • the amount of rosin is 0.1-50 wt-% of the total weight of the composition.
  • the amount of rosin may be from 0.1, 0.3, 0.5, 1, 1.5, 2,
  • the amount of rosin is 5-25 wt- % of the total weight of the composition.
  • the amount of rosin may be from 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5, 20,
  • an embodiment of the present disclosure provides a composition comprising rosin, in particular an alkali salt of rosin, and at least one solvent, such as alcohol, wherein the amount of rosin is 0.5-5 wt-% of the total weight of the composition, such as for example 1-3 wt% of the total weight of the composition.
  • the amount of rosin may be 1-3 wt-% of the total weight of the composition. In such a case, limiting the concentration of rosin to 1-3 wt-% of the total weight of the composition results in lower precipitation of rosin and enables effective antimicrobial activity of the rosin. It will be appreciated that rosin at a concentration of 3 wt-% is more effective in protecting the seeds as compared to rosin used at a concentration of 1 wt-% of the total weight of the composition, while bearing in mind that for some applications, 1 wt-% may be fully sufficient as a preferable amount of rosin.
  • the rosin comprises or consists essentially of abietic acid, dehydroabietic acid, palustric acid, isopimaric acid, pimaric acid, dihydroabietics acid, neoabietic acid, 13-b-7,9(11)- abietic acid, 8.12-abietic acid, 7,9(l l)-abietic acid, sandaracopimaric acid, 8,15-pimaric acid, 8,15-isopimaradien-18-oic acid, levopimaric acid and combinations thereof.
  • the rosin comprises at least 38 wt-% abietic acid, typically at least 40 wt-% abietic acid, and at least 15 wt-% dehydroabietic acid, based on the total weight of the rosin.
  • the rosin comprises 38- 45 wt-% abietic acid, 15-22 wt-% dehydroabietic acid, 8-12 wt-% palustric acid, possibly with 3-5 wt-% isopimaric acid, 3-5 wt-% pimaric acid, 3-5 wt-% dihydroabietics acid (group), and 3-5 wt-% neoabietic acid, based on the total weight of the rosin, and further optionally with minor amounts of other abietic acids or pimaric acids.
  • the at least one solvent is at least one alcohol.
  • the at least one alcohol enables increased solubility of the rosin.
  • the at least one alcohol is selected from ethanol and isopropanol. It may also be a mixture of ethanol and isopropanol, at various ratios, such as comprising ethanol and isopropanol.
  • ethanol, isopropanol and/or combination thereof are safe to be sprayed on plants or any part thereof, such as seeds.
  • the at least one alcohol may, in addition to those mentioned above, be selected from dimethyl sulfoxide, propylene glycol, glycerol, other polyalcohols, and their combinations.
  • the alcohol is used at a concentration of 5-70 wt-%.
  • the composition comprising rosin and at least one solvent, such as at least one alcohol, can be used for seed treatment in a concentrated or a diluted form.
  • the weight percentage of rosin and the at least one alcohol is in a range of 0.5-50 wt-%, more preferably 5-25 wt-%, and 5-70 wt-% of the total weight of the composition.
  • the amount of at least one alcohol may be from 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5,
  • other components can be added to the composition. More optionally, the other components (or additives) are added to alter a natural composition of ethanol, isopropanol and/or combination thereof, thereby making it more appropriate for use as a solvent. Furthermore, the other components can be added to improve the solubility of the composition. However, it is not desirable to use additives that could also impact growth of plants and/or pathogens on the plants (such as additives that promote growth of pathogens on plants). In addition to an increase in cost of the composition after addition of such other components or additives, repeated use is likely to impact plant growth and/or aggravate harmful environmental interactions, such as promote a harmful change in the atmospheric carbon dioxide concentration around the plants.
  • suitable additives can be for example surface active agents (for example up to 20 wt-%), agents modifying the viscosity of the composition, such as carboxy methyl cellulose and its derivatives (for example up to 20 wt-%), agents modifying the adsorption of the composition (for example up to 30 wt-%).
  • suitable additives include but are not limited to wetter-spreaders, stickers, emulsifiable oil activators, foliar nutrients, compatibility agents, drift retardants, foam retardants, buffers, plant penetrants, inverting agents, sinking agents, protectant binders, and stabilizing agents, each for example up to 20 wt-%.
  • a person skilled in the art is to able select a suitable additive depending on other components of the composition.
  • pH of the composition is 3-12. It will be appreciated that dissolution of rosin in at least one solvent is subjective to the pH of the solvent.
  • the pH has an effect on the solubility of the sodium salt of rosin into aqueous solutions. Indeed, when the sodium salt of rosin is dissolved in water, its pH is about 10-11. If the pH is lowered (for example by adding hydrochloric acid) to 7.5-8.5, the rosin precipitates. Thereafter, it reacts to form its free acid form, alcohol increasing the solubility. Furthermore, at a pH of 8, the rosin dissolves completely in the at least one alcohol. It is desirable that pH of the composition is adjusted to about 8 with the addition of a suitable acid or base.
  • a suitable acid or base includes, but is not limited to, 1 molar (1M) sodium hydroxide (NaOH) or 1M hydrochloric acid (HCI), respectively.
  • NaOH sodium hydroxide
  • HCI 1M hydrochloric acid
  • addition of such acids results in formation of precipitates in higher concentrations of the composition.
  • the precipitation may impact the actual concentration of rosin in the total composition, thereby impacting (such as reducing) the growth of pathogens.
  • the pH of the composition can thus be for example from 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10 or 10.5, 11, 11.5 up to 4, 4.5, 5, 5.5, 6, 6.5, 7, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5 or 12.
  • the present disclosure also provides the method of treating seeds.
  • the method comprises providing a solution of rosin and at least one solvent.
  • a commercially available solid tail-oil rosin (Forchem for90) is dissolved into the at least one solvent, preferably at least one alcohol, more preferably the at least one alcohol selected from ethanol and isopropanol.
  • the amount of rosin is 0.1-50 wt-% of the total weight of the composition.
  • the concentration of Forchem (For90) is 0.51-50 wt-%, more preferably at 5-25 wt-% of the total weight of the composition.
  • the concentration is provided to be applied externally to the seeds.
  • the at least one solvent used for dissolving the rosin can be selected from ethanol, isopropanol or a combination thereof.
  • the at least one solvent can also be selected from dimethyl sulfoxide, propylene glycol, glycerol, other polyalcohols, and their combinations, in addition to those mentioned above,
  • the method comprises providing a solution of rosin and the at least one solvent and subsequently, spraying the solution on the seeds. The seeds are sprayed with the provided solution of rosin and the at least one solvent in a predefined concentration.
  • spraying at least one of alcohols, selected from ethanol, isopropanol and/or combination thereof, even at substantially higher concentrations, is safer than watering plants with even low concentrations of ethanol, isopropanol and/or combination thereof.
  • spraying ethanol at a concentration of up to 70 % may not cause damage to the plant, while watering the plant even with a concentration ranging between 5- 25 % of ethanol may result in stressing (at 5 % concentration of ethanol) or death (at 25 % concentration of ethanol) of the plant.
  • the external application of the composition to the seeds comprises wetting, watering or brushing the seeds with the composition.
  • the method comprises drying the sprayed seeds.
  • the sprayed seeds are dried to evaporate the at least one alcohol from the provided solution of the rosin and the at least one solvent.
  • the water-insoluble free acid form of rosin forms a protective coating around the seed, which acts as an antifungal agent for the seeds.
  • the water-soluble alkali metal salt of rosin is absorbed in the surrounding ground or soil around the seed.
  • the alkali metal salt of rosin act as an antifungal agent for the soil surrounding the seed.
  • the use of the composition comprising rosin and at least one solvent for seed treatment and method thereof protects the seeds from the harmful effects of the pathogens such as fungi as well as the at least one solvent, such as the aforementioned organic solvents.
  • the composition comprising rosin and at least one alcohol is used for seed treatment.
  • the composition may also be used as potential fungicide, bactericide and so forth, owing to the antimicrobial properties of the rosin and the disinfectant properties of the at least one alcohol.
  • the composition is applied externally to the seeds. More specifically, the application is for example by way of spraying, wetting, watering or brushing the seeds with the composition. Spraying the seeds with the composition typically protects the seeds from the harmful effects of alcohol on the plants as alcohol evaporates over a period of time, leaving behind a protective coating of the rosin over the seed.
  • the pH of 7.5-8.5 protects the seeds from a potential attack from the different species of fungi in the environment (such as ground).
  • the composition comprising both the forms of rosin, i.e. the free rosin acid and the sodium salt of rosin provides protection to the seeds and fungicidal effect on the surrounding ground (soil or water, such as in hydroponics arrangement for plant growth) respectively.
  • the rosin was tested in seed treatment as follows. A mixture of free rosin acid (For90) and ethanol comprising 20 wt-% of free rosin acid was sprayed on oat seeds and the ethanol was allowed to evaporate. A shiny, dense coating was formed on the surface of the seeds, after evaporation of ethanol. Thereafter, the seeds were allowed to germinate. The thus treated seeds were compared to untreated seeds, and both germinated in identical manner. Thus the seed treatment did not affect the germination of the seeds.
  • the antifungal properties of the composition were used for preventing growth of fungus on the plants.
  • different species of fungi were grown in potato dextrose agar (indicated as 'PDA' hereafter) powder (comprising agar and nutrients for growth of fungi) for a period of 4 days at a temperature of +18°C.
  • the cultured species of the fungi having a diameter of 7 mm or more were inoculated and their growth was monitored in a sample comprising dried form of rosin, optionally the sodium salt of rosin, and PDA.
  • Such sample of rosin and PDA was dissolved in cold water and pH of the resulting mixture was adjusted to about 8, by adding a suitable acid or a base thereto.
  • the resulting first sample was heated in an autoclave maintained at a predefined temperature of 121 °C. It was observed that, at such a high temperature the precipitates formed in the composition had ceased to exist. However, the precipitation in the composition continued to occur at room temperature (about 20 °C).
  • the fungus was selected from a group comprising Botrytis cinerea, Pyrenophora teres, Bipolaris sorokiniana, Stagonospora nodorum, Fusarium graminearum and Fusarium avenaceum . It will be appreciated that other species of fungi having similar characteristics including, but not limiting to, colour, formation of spores, structure and density, may also be selected.
  • the six species of fungi were grown under dark conditions at +18 °C and at 3 different concentrations of rosin, i.e. 0 wt-% (i.e. absence of the rosin in the first sample or "control"), 1 wt-% and 3 wt-%.
  • the fast-growing species of fungi namely, Botrytis cinerea and Fusarium avenaceum
  • Botrytis cinerea and Fusarium avenaceum also demonstrated a significant reduction in growth at 3 wt-% concentration of rosin.
  • the growth of fungi was significantly reduced or substantially prohibited for all the aforementioned species of fungi.
  • higher concentrations of rosin had an effect on weakening the spreading and conservation of the aforementioned species of the fungi.
  • the rosin has an impact not only to reduce the growth of the fungi, but also in modifying structure and formation of spores of some species of fungi.
  • the growth of fungus Fusarium gram inearum was abundant and lint-like in the control sample, while in the sample with rosin, the growth of the fungi was dense and observed to be located only at the surface of the sample.
  • fungus Botrytis cinerea formed several sclerotium in the control sample, while the presence of rosin prohibited the formation of sclerotium until the 8 th day and 14 th day. However, the formation of sclerotium was observed after the 14 th day at the 1 wt-% concentration of rosin.
  • colour of some species of the fungi may be a measure of the growth of fungi in a sample.
  • the fungus Bipolaris sorokiniana exhibited black spots containing a significant number of conidium, while in the samples comprising rosin at any concentration, the fungal growth was lighter in colour and contained no or negligible conidium.
  • presence of the rosin significantly limited the formation of spores in fungal species, such as Pyrenophora teres, Stagonospora nodorum and Fusarium avenaceum .
  • FIGs. 1A, IB and 1C The test results are shown in FIGs. 1A, IB and 1C, as graphical representations of average growth of fungi.
  • the different species of fungi were grown in different concentrations of compositions under dark conditions, as explained above, for a predefined period of time.
  • the Y-axis (vertical axis) of the graphs represent the average growth of fungi in millimetres (mm).
  • the X-axis (horizontal axis) of the graphs represent the different species of fungi Botrytis cinerea (depicted as species code ' ), Pyrenophora teres (depicted as '2'), Bipolaris sorokiniana (depicted as '3'), Stagonospora nodorum (depicted as '4'), Fusarium graminearum (depicted as '5') and Fusarium avenaceum (depicted as '6').
  • the left-most column is the result at 4 days
  • the middle column is the result at 8 days
  • the right-most column the result at 14 days. The same applies for FIG.
  • FIG. 1A the different species of fungi grew normally under control conditions, i.e. when the concentration of rosin was 0 wt-%.
  • addition of rosin in each sample of fungi resulted in a significant impact on the growth of fungus, as shown in FIGs. IB and 1C.
  • rosin prohibited the growth of all species of fungi until 8 days and 14 days.
  • only the fastest growing species of fungi such as Botrytis cinereal and Fusarium avenaceum were able to grow despite the stronger concentration of rosin.
  • fungi namely, Botrytis cinereal, Pyrenophora teres, Bipolaris sorokiniana and Fusarium avenaceum
  • Botrytis cinereal Pyrenophora teres
  • Bipolaris sorokiniana Fusarium avenaceum
  • Fusarium avenaceum four species of fungi, namely, Botrytis cinereal, Pyrenophora teres, Bipolaris sorokiniana and Fusarium avenaceum.
  • NUV near visible ultraviolet
  • FIGs. 2A to 2E The results are shown in FIGs. 2A to 2E as graphical representations.
  • the Y-axis (vertical axis) of the graphs represent the average growth of fungi in millimetres (mm).
  • the X-axis (horizontal axis) of the graphs represent the different species of fungi, namely Botrytis cinerea (depicted as species code ' ), Pyrenophora teres (depicted as '2'), Bipolaris sorokiniana (depicted as '3') and Fusarium avenaceum (depicted as '6').
  • the average growth of fungi was recorded for a period of 3 and 6 days after anthesis (DAA) at each concentration of 0 wt-%, 0.5 wt-%, 1 wt-%, 2 wt-% and 3 wt-% of rosin as shown in FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D and FIG. 2E respectively.
  • DAA anthesis
  • the left column is the result at 3 days and the right column the result at 6 days.
  • FIG. 2A shows that the different species of fungi grew normally under control conditions, i.e. when the concentration of rosin is 0 wt-%.
  • compositions of the invention were tested in field trials by evaluating germination and protection against plant pathogenic fungi when seeds of spring barley (2 varieties), spring wheat and common oat (2 varieties) had been treated with the compositions of the invention prior sowing.
  • compositions tested contained varying amounts of the sodium salt of rosin acid in either water (A) or 96% ethanol (B). Water and 96% ethanol were used as reference compositions. In addition, untreated seeds were included in the study as controls. The numbering of the treatments and the amounts of active ingredient (sodium salt of rosin acid) per seed weight were as follows: Table 1 . Numbering of treatments
  • Leaf blotch, net blotch or leaf stripe of the plants were also evaluated again from the plant samples. Heads with smut ( Ustilago sp) symptoms were counted per plot and plants with leaf stripe symptoms were counted from 1.5 m 2 area. The results are summarized in Table 2.
  • Ethanol-based products were effective against net blotch and leaf stripe of barley.
  • the highest concentration of water-based product A had a remarkable effect on leaf stripe of barley compared to the untreated or water-treated seeds. It also provided slightly better effect against leaf blotch of oat than ethanol-based products.
  • Results for both varieties of barley are summarized in Table 3. Table 3 . Results for barley
  • Figu re 4 The effect on leaf stripe of barley is further illustrated in Figu re 4 , wherein the results obtained by different concentrations of Product A compared to untreated seeds (1) or water-treated seeds (2) in preventing leaf stripe of barley are shown.
  • the composition of the invention has an antifungal effect, which increases with increasing dose of rosin.
  • Ethanol-based products were effective against smut fungi. All concentrations of Product A reduced heads with smut symptoms in oat when compared to untreated or water-treated seeds, the highest concentration of product A having a significant impact. Ethanol-based products were also effective against smut fungi in oat, as can be seen in Table 4. Table 4. Results for oat
  • Figu re 5 The effect against smut fungi in oat is further illustrated in Figu re 5 , wherein the results obtained by different concentrations of Product A compared to untreated seeds (1) or water-treated seeds (2) in preventing heads with smut in oats are shown.
  • the composition of the invention has an effect against formation of heads with smut, and the effect increases with increasing dose of the active agent.
  • a solution of rosin and at least one solvent is provided.
  • the solution is sprayed on the seeds.
  • the seeds sprayed with the solution are dried.
  • steps 302 to 306 are only illustrative and other alternatives can also be provided where one or more steps are added, one or more steps are removed, or one or more steps are provided in a different sequence without departing from the scope of the claims herein.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Toxicology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

L'invention concerne une utilisation d'une composition comprenant de la rosine et au moins un solvant pour le traitement de semences. L'invention concerne en outre un procédé de traitement de semences à l'aide de la composition susmentionnée. Le procédé de traitement de semences consiste à utiliser une solution de rosine et au moins un solvant, à pulvériser la solution sur les semences et à sécher les semences pulvérisées.
PCT/FI2019/050644 2018-09-07 2019-09-09 Utilisation de rosine pour le traitement de semences WO2020049228A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP19773134.2A EP3846622A1 (fr) 2018-09-07 2019-09-09 Utilisation de rosine pour le traitement de semences
CA3111659A CA3111659A1 (fr) 2018-09-07 2019-09-09 Utilisation de rosine pour le traitement de semences
AU2019335556A AU2019335556A1 (en) 2018-09-07 2019-09-09 Use of rosin for seed treatment

Applications Claiming Priority (2)

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FI20185747A FI20185747A1 (en) 2018-09-07 2018-09-07 USE OF ROSIN FOR SEED TREATMENT
FI20185747 2018-09-07

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AR (1) AR117641A1 (fr)
AU (1) AU2019335556A1 (fr)
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FI (1) FI20185747A1 (fr)
TW (1) TW202021450A (fr)
WO (1) WO2020049228A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021152217A1 (fr) * 2020-01-31 2021-08-05 Woimet Oy Utilisation de colophane pour le traitement de plantes

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Publication number Priority date Publication date Assignee Title
US2128973A (en) * 1935-06-27 1938-09-06 Du Pont Protective composition for fruits and the like
GB936584A (en) * 1959-09-03 1963-09-11 Albert Ag Chem Werke Seed-treating compositions
KR100346313B1 (ko) * 1999-10-18 2002-07-26 이항섭 고추탄저병 방제제
WO2016116668A1 (fr) * 2015-01-20 2016-07-28 Repolar Pharmaceuticals Oy Dispersion microbicide huile dans eau
CN106259564A (zh) 2016-08-04 2017-01-04 巢湖市种子协会 一种轻度盐碱地玉米播种用种子包衣剂
EA027877B1 (ru) 2015-09-30 2017-09-29 Общество С Ограниченной Ответственностью "Кохим" Состав для предотвращения растрескивания стручков рапса

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Publication number Priority date Publication date Assignee Title
US2128973A (en) * 1935-06-27 1938-09-06 Du Pont Protective composition for fruits and the like
GB936584A (en) * 1959-09-03 1963-09-11 Albert Ag Chem Werke Seed-treating compositions
KR100346313B1 (ko) * 1999-10-18 2002-07-26 이항섭 고추탄저병 방제제
WO2016116668A1 (fr) * 2015-01-20 2016-07-28 Repolar Pharmaceuticals Oy Dispersion microbicide huile dans eau
EA027877B1 (ru) 2015-09-30 2017-09-29 Общество С Ограниченной Ответственностью "Кохим" Состав для предотвращения растрескивания стручков рапса
CN106259564A (zh) 2016-08-04 2017-01-04 巢湖市种子协会 一种轻度盐碱地玉米播种用种子包衣剂

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ARRUDA, G.O.S. F. DE ET AL., CIENCIA FLORESTAL, vol. 18, no. 1/2, 2007, pages 75 - 84, ISSN: 0103-9954

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021152217A1 (fr) * 2020-01-31 2021-08-05 Woimet Oy Utilisation de colophane pour le traitement de plantes

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AR117641A1 (es) 2021-08-18
TW202021450A (zh) 2020-06-16
FI20185747A1 (en) 2020-03-08
AU2019335556A1 (en) 2021-05-13
CA3111659A1 (fr) 2020-03-12
EP3846622A1 (fr) 2021-07-14

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