WO2020049095A1 - Procédé d'enrichissement d'une biomasse en protéines - Google Patents
Procédé d'enrichissement d'une biomasse en protéines Download PDFInfo
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- WO2020049095A1 WO2020049095A1 PCT/EP2019/073690 EP2019073690W WO2020049095A1 WO 2020049095 A1 WO2020049095 A1 WO 2020049095A1 EP 2019073690 W EP2019073690 W EP 2019073690W WO 2020049095 A1 WO2020049095 A1 WO 2020049095A1
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- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
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- A21D2/26—Proteins
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- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
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- A—HUMAN NECESSITIES
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- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
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- A23J3/227—Meat-like textured foods
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- A—HUMAN NECESSITIES
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- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
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- A23K—FODDER
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
- A23L29/281—Proteins, e.g. gelatin or collagen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/195—Proteins from microorganisms
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/02—Algae
- A61K36/04—Rhodophycota or rhodophyta (red algae), e.g. Porphyra
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
Definitions
- the present invention relates to a process for enriching proteins in a biomass of red single-celled algae such as Galdieria and the biomass thus obtained.
- soybeans generally used in the form of oil cakes, a secondary solid residue obtained from the extraction of oil.
- oil cakes are generally imported from countries which cultivate an intensive soybean culture to the detriment of other plants source of biodiversity.
- GM genetically modified soybean varieties, which are found mixed with non-GMO soybeans in oil cakes, which does not meet growing demand for food of origin non-GMO vegetable.
- Spirulina like chlorella, however has the disadvantage of low productivity which does not allow the transition to a culture in high yield fermenter. If their culture meets local and limited demand for traditional food supplements, it does not meet a broader industrial production objective, economically viable, of a source of food protein whose qualities will allow it to replace tomorrow usual sources such as soya in animal and human food.
- Microalgae such as Galdieria are also studied with regard to their nutritional characteristics (Graziani & al., 2013). Protein extracts from microalgae such as Auranthiochitrium known to produce oils rich in polyunsaturated fatty acids are also described (WO 2017/132407, WO 2017/019125, WO 2016/015013). They are however implemented on a defatted biomass, that is to say after having extracted and eliminated the fatty fraction of the cultivated biomass. This is also what is used for the recovery of chlorella proteins (WO 2016/120548, WO 2016/00194).
- GMOs genetically modified microorganisms
- the present invention relates to a method for producing a protein-rich biomass, the method comprising the steps of
- the fraction of biomass recovered in c. can then be dried.
- the invention also relates to the biomass thus obtained, its use for human or animal consumption, as well as the foods and food compositions which contain it.
- Figure 1 represents the protein content (% of acids animated in the Dry Matter) for a spirulina biomass and for a biomass of Galdieria sulphuraria strain UTEX 2919 before and after treatment according to the invention for 1 cycle (PL1) or 4 cycles (PL4) of treatment.
- FIG. 2 represents the amino acid composition (in g / 100g of DM) for a spirulina biomass and for a biomass of Galdieria sulphuraria strain UTEX 2919 before and after treatment according to the invention for 1 cycle (PL1) or 4 cycles ( PL4) of processing.
- the present invention relates to a method for producing a biomass of lysed microorganisms rich in proteins, the method comprising the steps of b.1. suspension of a biomass of lysed microorganisms,
- microorganisms essentially microorganisms which accumulate glycogen soluble in water, such as yeasts or protists which accumulate glycogen soluble in water during their growth, in particular under industrial conditions of culture by fermentation with biomass accumulation
- the method is particularly suitable for a biomass of microorganisms producing phycobiliproteins.
- the process advantageously makes it possible to make use of the biomass remaining after extraction of the phycobiliproteins, a by-product of the production of these molecules of interest.
- ARU unicellular red algae
- Rhodophytes in particular of the sub-division of Cyanidiophytina, preferably of the class of Cyanidiophyceae, in particular of the order of Cyanidiales, more particularly of the families of Cyanidiaceae or Galdieriaceae, even more particularly of the genera Cyanidioschyzon, Cyanidium or Galdieria.
- the ARUs are chosen from the Cyanidioschyzon species merolae 10D, Cyanidioschyzon merolae DBV201, Cyanidium caldarium, Cyanidium daedalum, Cyanidium maximum, Cyanidium partitum, Cyanidium rumpens, Galdieria daedala, Galdieria maxima, Galdieria partita or Galdieria sulphuraria, more preferentially of the species Galdieria sulphuraria.
- the method is implemented with a lysed biomass of which at least 70% of the cells are lysed, more preferably at least 80% of lysed cells, even more preferably 90%, even more preferably 95%.
- the method comprises a preliminary step a. for lysis of a biomass of microorganisms previously cultivated.
- Step a. lysis can be done by any means of cell lysis known to those skilled in the art. Mention will be made in particular of grinding or enzymatic lysis. Preferably, cell lysis is carried out by grinding, this method being particularly preferred for the lysis of ARUs, in particular of the species Galdieria sulphuraria.
- the lysis by grinding is carried out on a biomass of “dry” cells, in particular on a biomass of fresh cells separated from the fermentation medium after culture by any separation method well known to those skilled in the art, in particular by filtration or centrifugation.
- lysis by grinding is carried out on a biomass of cells previously lyophilized or dried for their conservation.
- Step b.1. of suspension makes it possible to obtain a suspension of the lysed biomass.
- the suspension obtained in step b1 generally has between 1% and 40% of dry matter, or more. Preferably it will have between 20% and 30% of dry matter.
- Step b.2. is implemented by adding an acid to obtain a suspension whose aqueous phase has a pH less than or equal to 5.
- a pH less than or equal to 4 The pH is adjusted by adding a strong or weak acid, mineral or organic, in the form of a solid or solution, the amount of acid added being determined by the pH of the suspension to be treated and the pH value that a person skilled in the art will seek to obtain.
- mineral acids well known to those skilled in the art, there will be mentioned more particularly hydrochloric acid, sulfuric acid and phosphoric acid.
- organic acids well known to those skilled in the art, there will be mentioned in particular acetic acid, citric acid, tartaric acid, lactic acid, preferably citric acid.
- acids used are acids authorized for food use, in particular phosphoric acid, sulfuric acid, citric acid or tannic acid.
- the acid suspension obtained by step b.2. preferably has a dry matter content greater than or equal to 1%, advantageously more than 2% of dry matter, preferably from 3 to 30% of dry matter, more preferably from 4 to 15% of dry matter, even more preferably from 5 to 12%, even more preferably from 6 to 10%.
- steps b.1. and b.2. are consecutive, step b.2 being carried out directly after step b.1 without any other biomass treatment step such as a defatting step, for example.
- the suspension, or dilution of the suspension is followed by the addition of acid, in solid or liquid form.
- steps b.1. and b.2. are carried out simultaneously by adding an appropriate amount of an acidic solution to obtain both the desired pH less than or equal to 5 and the desired dry matter content.
- step a Depending on the lysed biomass recovered from step a., In particular whether the lysis was carried out on a cell suspension in water or on a so-called “dry” biomass, the skilled person will be able to add the amounts of water and acid necessary for obtaining a suspension with the desired dry matter content and at the desired pH, whether these additions are made in two stages or in one single step.
- Step c. is implemented by any separation means known to a person skilled in the art for separating the solid cell lysis residues from the aqueous solution from the acid suspension comprising the lysed cells, in particular by filtration or by centrifugation.
- separation means known to a person skilled in the art for separating the solid cell lysis residues from the aqueous solution from the acid suspension comprising the lysed cells, in particular by filtration or by centrifugation.
- tangential filtration for example tangential filtration on ceramic membranes or organic membranes such as hollow polyethersulfone fibers.
- Front filtration can also be used (plate filter or filter press).
- the suspension / acidification / recovery cycle (b.1./b.2./c.) Can be repeated several times on the fraction of lysed biomass insoluble at pH less than or equal to 5 recovered in c. so as to exhaust it in soluble elements at acidic pH.
- the suspension / acidification / recovery cycle is repeated 1, 2 or 3 times on the lysed biomass recovered in c.
- the fraction of protein-enriched biomass recovered in c. can then be dried using any known method of drying.
- the mother liquors of the suspension are generally recovered in order to be treated so as to extract therefrom soluble components at acid pH which can be valued on their side.
- This is particularly the case for phycocyanins when the microorganisms are microorganisms producing phycobiliproteins as defined above.
- Such a process is described in particular in the patent application PCT / EP2018 / 058294 filed on March 30, 2018.
- the biomass obtained generally has at least 60% of proteins (evaluated by the determination of total nitrogen, N * 6.25) relative to the dry matter, preferably more than 65%, even more preferably more than 68%, more than 70% or even more than 80%.
- the biomass also preferably has a total sugar content of less than 20% relative to the dry matter, more preferably less than 10%, less than 5%.
- the biomass obtained also preferably has a glycogen content of less than 10% relative to the dry matter, more preferably less than 5%, or even less than 1%, even more preferably less than 0.1%.
- the lysed biomass insoluble at pH less than or equal to 5 may have a fat content, in particular lipids, which may be at least 5%, or even at least 10% relative to the material. dry, or at least 15%, at least 16%, up to 20%.
- the fat composition will also depend on the microorganisms.
- the lysed biomass according to the invention generally comprises polyunsaturated fatty acids of omega 3, 6 and 9 type, in particular oleic, palmitic and alpha-linolenic acids which represent at least 30% of the fatty matter, preferably 40% , even more preferably 50% of the fat.
- proteins can be expressed both with respect to the proteins themselves and with respect to the amino acids contained in said proteins.
- proteins is meant, according to the present invention, any protein, peptide or amino acid insoluble at an acid pH less than or equal to 5.
- the present invention also relates to the biomass as defined above, capable of being obtained by the method according to the invention.
- glycogen preferably less than 10% of glycogen, preferably less than 5%, more preferably less than 1% or even more preferably less than 0.1%, and
- Another subject of the invention is the use of a biomass as described above in the cosmetic, pharmaceutical, human or animal food fields.
- a protein-enriched biomass according to the invention for improving the performance of animals.
- This improvement in performance can be assessed, in particular, by measuring consumption, weight gain or even the "Feed Conversion Ratio".
- farmed animal in particular grazing animals (especially cattle raised for meat, milk, cheese and leather; sheep raised for meat, wool and cheese; goats), pigs, rabbits, poultry (chickens, hens, turkeys, ducks, geese and others), equines (ponies, horses, foals), intended to support human activities (transport, leisure) or their food , aquatic animals (eg fish, shrimp, shellfish (especially oysters and mussels)).
- grazing animals especially cattle raised for meat, milk, cheese and leather; sheep raised for meat, wool and cheese; goats
- poultry chickens, hens, turkeys, ducks, geese and others
- equines ponies, horses, foals
- aquatic animals eg fish, shrimp, shellfish (especially oysters and mussels)
- the invention also relates to a food, or food composition, for humans or animals, comprising a biomass according to the invention as described above.
- Food means any composition which can be used for food for humans or animals.
- the food may comprise only biomass, dried or not, transformed or not, or biomass, dried or not, transformed or not, mixed with any other additive, vehicle or support, used in the field.
- food or feed such as food preservatives, colorings, flavor enhancers, pH regulators, or pharmaceutical additives such as growth hormones, antibiotics.
- the present invention relates in particular to animal feed and more particularly to farm animals.
- These foods are usually in the form of flour, granules or soup in which the biomass according to the invention is incorporated.
- “Animal food” means anything that can be used to feed animals.
- feed may include, in addition to algal biomass, a nutritional base and nutritional additives.
- Most of the animal's food ration then consists of the "nutritional base” and the algal biomass. This base is constituted, for example, by a mixture of cereals, proteins and fats of animal and / or vegetable origin.
- the nutritional bases for animals are suitable for feeding these animals and are well known to those skilled in the art.
- these nutritional bases include, for example, corn, wheat, peas and soy. These nutritional bases are adapted to the needs of the different animal species for which they are intended. These nutritional bases may already contain nutritional additives such as vitamins, minerals and amino acids.
- Additives used in animal feed can be added to improve certain characteristics of food, for example to enhance the taste, to make the raw materials of animal feed more digestible or to protect animals. They are frequently used in large scale intensive farming.
- the additives used in animal feed are available, in particular, in the following subcategories (source EFSA):
- additives for example, preservatives, antioxidants, emulsifiers, stabilizers, acidity regulators and additives for silage;
- - nutritional additives for example, vitamins, amino acids and trace elements
- zootechnical additives for example, digestibility enhancers, intestinal flora stabilizers;
- the invention relates to feed for farm animals comprising between 1 and 60%, preferably between 1 and 20%, quite preferably between 3 and 8% of a dried biomass obtained by the method according to the invention.
- the invention relates to feed for farm animals comprising between 1 and 40%, preferably between 5 and 10% of an undried biomass obtained by the process of the invention.
- the food is intended for farm animals, especially cattle, sheep, pigs, rabbits, poultry and horses.
- the food is intended for aquatic animals, in particular for fish, at least up to the fry stage, including farmed and ornamental fish.
- the food is intended for domestic animals, pets and / or leisure animals and NACs.
- the food composition is intended for humans.
- the subject of the invention is also a cosmetic, nutraceutical or pharmaceutical composition for humans or animals comprising a biomass according to the invention as described above.
- the cosmetic, nutraceutical or pharmaceutical composition may only comprise biomass, dried or not, transformed or not, or biomass, dried or not, transformed or not, mixed with any other additive, vehicle or support, used in the field of cosmetics or pharmacy such as, for example, preservatives, dyes, pH regulators.
- the invention also relates to the use of biomass as described above in therapy, as well as in the prevention and treatment of malnutrition.
- the biomasses treated by the process according to the invention are biomasses of spirulina (Arthrospira platensis) and of Galdieria sulphuraria strain UTEX 2919 (UTEX Culture Collection of Algae, 205 W. 24th St, Biological Labs 218, The University of Texas at Austin ( A6700), Austin, TX 78712 USA) cultivated according to the usual methods for cultivating these strains.
- the lysed biomass has a lysed cell content of 95%
- the dry matter content is 10%
- the enrichment process is carried out by repeating a rinsing of the pellet of the crushed biomass under an acid pH condition.
- the operation involves the use of a centrifuge, here described by the use of a SORVALL RC5B plus and a Sorvall SLA 3000 fixed angle rotor.
- the crushed biomass is centrifuged at 12,000 xg for 15 min, allowing the fraction to be pelleted as much as possible insoluble.
- the “soluble fraction” supernatant is removed and replaced with water.
- the pellet is resuspended before repeating the centrifugation operation, and the pH is checked and adjusted if necessary to a value below pH 4.8. This process, thus repeated 3 to 4 times, maximizes the protein and lipid enrichment of the insoluble fraction.
- Total proteins are estimated using the Kjeldahl method known to those skilled in the art.
- the amino acid profile by acid hydrolysis is carried out according to the ISO 13903: 2005 method; EU 152/2009.
- the analysis of the lipid content and of the composition is done by GC / FID and internal calibration known to those skilled in the art.
- the sugar profile is determined by ion chromatography - pulsed amperometric.
- the protein content of the different biomasses is shown in Figure 1. If we look in detail at the amino acid composition in the different products shown in Figure 2 we can see an overall increase in all amino acids on the phases heavy, and amounts greater than or equal to those of Spirulina for most of them except for alanine.
- C-PC and APC phycocyanin
- Phycocyanin is known to be a molecule with strong antioxidant power.
- the fatty acid composition shows significant amounts of omega 3, 6 and 9 type unsaturated fatty acids, in the form of polar lipids, representing more than 50% of the fatty mass which represents approximately 5% to 20% of the mass the heavy phase (Table 3).
- the starting biomass comprises more than 70% of proteins compared to the DM. After three treatments according to the invention, a biomass is obtained with more than 80% of proteins, an increase of more than 14%.
- the heavy phase enriched in proteins thus obtained can be subsequently enzymatically treated with proteases in order to obtain a protein hydrolyzate and to increase the solubility and digestibility of the product.
- ANC Nutritional Intakes
- the protein-enriched fraction produced by the process described in this invention can be consumed directly in the form of a cold pressed tablet, in powder form in the form of a capsule, or directly in powder form to be mixed with other ingredients.
- This fraction can also be integrated into other food preparations which can be used for the manufacture of cake bread, biscuit or other pastry, or bars of cereals, or any preparation in replacement of the traditional flour in order to enrich in proteins the food thus prepared.
- This protein fraction can also be incorporated into preparations intended to serve as a substitute for meat such as vegetable burgers, or in protein sticks in surimi manner, these proteins being able to be textured or used as such.
- Another example of use would be in the form of powder to be added to drinks such as Smoothie or Shake.
- chickpea flour (26%), seaweed powder (25%), onion, carrot, wheat gluten (3%), lemon juice, yeast extract, potato starch, vegetable oil (sunflower) ), turmeric, garlic, acidity regulator (potassium chloride), antioxidant, potato fibers, thickener (carrageenan), starch (corn, wheat), emulsifier (guar gum).
- Dairy product enriched with algae proteins Dairy product enriched with algae proteins.
- the protein-enriched alga fraction is added to the coagulated milk proteins before pressing the dough.
- the percentage of algae fraction enriched in protein can vary from 1% to 50%. In this case the limiting factor will be linked to the appearance of the final product and in particular to its texture and the resistance to cutting.
- Powder for beverages enriched with algae proteins Powder for beverages enriched with algae proteins.
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Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
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CN201980057719.XA CN112639072A (zh) | 2018-09-05 | 2019-09-05 | 富集具有蛋白质的生物质的方法 |
AU2019334563A AU2019334563A1 (en) | 2018-09-05 | 2019-09-05 | Method for enriching a biomass with proteins |
BR112021004196-6A BR112021004196A2 (pt) | 2018-09-05 | 2019-09-05 | método para enriquecer uma biomassa com proteínas |
JP2021512429A JP2021534805A (ja) | 2018-09-05 | 2019-09-05 | バイオマスをタンパク質で強化する方法 |
EP19762172.5A EP3847237A1 (fr) | 2018-09-05 | 2019-09-05 | Procédé d'enrichissement d'une biomasse en protéines |
US17/273,678 US20210340490A1 (en) | 2018-09-05 | 2019-09-05 | Method for enriching a biomass with proteins |
CA3111673A CA3111673A1 (fr) | 2018-09-05 | 2019-09-05 | Procede d'enrichissement d'une biomasse en proteines |
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FR1857950 | 2018-09-05 | ||
FR1857950A FR3085386B1 (fr) | 2018-09-05 | 2018-09-05 | Procede d'enrichissement d'une biomasse en proteines |
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WO2020049095A1 true WO2020049095A1 (fr) | 2020-03-12 |
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EP (1) | EP3847237A1 (fr) |
JP (1) | JP2021534805A (fr) |
CN (1) | CN112639072A (fr) |
AU (1) | AU2019334563A1 (fr) |
BR (1) | BR112021004196A2 (fr) |
CA (1) | CA3111673A1 (fr) |
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WO2021195708A1 (fr) * | 2020-03-31 | 2021-10-07 | v2food Pty Ltd | Agents colorants alimentaires |
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WO2023085922A1 (fr) * | 2021-11-15 | 2023-05-19 | Ful Foods B.V. | Procédé de production d'une composition, composition d'extrait hydrosoluble et composition d'extrait non hydrosoluble, chaque composition étant une composition à base de micro-organismes contenant des phycobiliprotéines |
WO2023218060A1 (fr) * | 2022-05-13 | 2023-11-16 | Coöperatie Koninklijke Cosun U.A. | Particules à base de plantes ou de champignons chargées avec une protéine |
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2018
- 2018-09-05 FR FR1857950A patent/FR3085386B1/fr active Active
-
2019
- 2019-09-05 CN CN201980057719.XA patent/CN112639072A/zh active Pending
- 2019-09-05 BR BR112021004196-6A patent/BR112021004196A2/pt unknown
- 2019-09-05 US US17/273,678 patent/US20210340490A1/en active Pending
- 2019-09-05 EP EP19762172.5A patent/EP3847237A1/fr active Pending
- 2019-09-05 CA CA3111673A patent/CA3111673A1/fr active Pending
- 2019-09-05 JP JP2021512429A patent/JP2021534805A/ja active Pending
- 2019-09-05 WO PCT/EP2019/073690 patent/WO2020049095A1/fr unknown
- 2019-09-05 AU AU2019334563A patent/AU2019334563A1/en active Pending
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Cited By (3)
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WO2021195708A1 (fr) * | 2020-03-31 | 2021-10-07 | v2food Pty Ltd | Agents colorants alimentaires |
CN115715152A (zh) * | 2020-03-31 | 2023-02-24 | V2食品股份有限公司 | 食品着色剂 |
AU2021247417B2 (en) * | 2020-03-31 | 2023-08-24 | v2food Pty Ltd | Food colouring agents |
Also Published As
Publication number | Publication date |
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FR3085386A1 (fr) | 2020-03-06 |
AU2019334563A1 (en) | 2021-03-25 |
CA3111673A1 (fr) | 2020-03-12 |
FR3085386B1 (fr) | 2022-10-07 |
JP2021534805A (ja) | 2021-12-16 |
CN112639072A (zh) | 2021-04-09 |
EP3847237A1 (fr) | 2021-07-14 |
US20210340490A1 (en) | 2021-11-04 |
BR112021004196A2 (pt) | 2021-05-25 |
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