Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
  • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
  • A61K31/415—1,2-Diazoles
  • A61K31/4155—1,2-Diazoles non condensed and containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
  • A61K31/4192—1,2,3-Triazoles
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
  • A61K31/4196—1,2,4-Triazoles
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
  • A61K31/42—Oxazoles
  • A61K31/422—Oxazoles not condensed and containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
  • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
  • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
  • A61P31/20—Antivirals for DNA viruses
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/44—Iso-indoles; Hydrogenated iso-indoles
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/52—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring condensed with a ring other than six-membered
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
  • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
  • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
  • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems

Definitions

• Hepatitis B is one of the world’s most prevalent diseases, being listed by National Institute of Allergy and Infectious Diseases (NIAID) as a High Priority Area of Interest. Although most individuals resolve the infection following acute symptoms, approximately 30% of cases become chronic. 350-400 million people worldwide are estimated to have chronic hepatitis B, leading to 0.5-1 million deaths per year, due largely to the development of hepatocellular carcinoma, cirrhosis and/or other complications.
• NIAID National Institute of Allergy and Infectious Diseases
• Hepatitis B is caused by infection with Hepatitis B virus (HBV), which is a noncytopathic, liver tropic DNA virus belonging to Hepadnaviridae family.
• HBV Hepatitis B virus
• Pregenomic (pg) RNA is the template for reverse transcriptional replication of HBV DNA.
• the encapsidation of pg RNA, together with viral DNA polymerase, into a nucleocapsid is essential for the subsequent viral DNA synthesis. Inhibition of pg RNA encapsidation may block HBV replication and provide a new therapeutic approach to HBV treatment.
• a capsid inhibitor acts by inhibiting the expression and/or function of a capsid protein either directly or indirectly: for example, it may inhibit capsid assembly, induce formation of non-capsid polymers, promote excess capsid assembly or misdirected capsid assembly, affect capsid stabilization, and/or inhibit RNA encapsidation.
• a capsid inhibitor may also act by inhibiting capsid function in one or more downstream events within the replication process, such as, but not limited to, viral DNA synthesis, transport of relaxed circular DNA (rcDNA) into the nucleus, covalently closed circular DNA (cccDNA) formation, virus maturation, budding and/or release.
• a limited number of drugs are currently approved for the management of chronic hepatitis B, including two formulations of alpha-interferon (standard and pegylated) and five nucleoside/nucleotide analogues (lamivudine, adefovir, entecavir, telbivudine, and tenofovir) that inhibit HBV DNA polymerase.
• the first-line treatment choices are entecavir, tenofovir and/or peg-interferon alfa-2a.
• peg-interferon alfa-2a achieves desirable serological milestones in only one third of treated patients, and is frequently associated with severe side effects.
• Entecavir and tenofovir are potent HBV inhibitors, but require long-term or possibly lifetime administration to continuously suppress HBV replication, and may eventually fail due to emergence of drug-resistant viruses. There is thus a pressing need for the introduction of novel, safe, and effective therapies for chronic hepatitis B.
• Hepatitis D virus is a small circular enveloped RNA virus that can propagate only in the presence of HBV.
• HDV requires the HBV surface antigen protein to propagate itself. Infection with both HBV and HDV results in more severe complications compared to infection with HBV alone. These complications include a greater likelihood of experiencing liver failure in acute infections and a rapid progression to liver cirrhosis, with an increased chance of developing liver cancer in chronic infections.
• hepatitis D In combination with hepatitis B, hepatitis D has the highest mortality rate of all the hepatitis infections.
• the routes of transmission of HDV are similar to those for HBV. Infection is largely restricted to persons at high risk of HBV infection, particularly injecting drug users and persons receiving clotting factor concentrates.
• inhibition of pg RNA encapsidation may offer certain therapeutic advantages for treatment of hepatitis B and/or hepatitis D.
• inhibition of pg RNA encapsidation may complement the current medications by providing an option for a subpopulation of patients that do not tolerate or benefit from the current medications.
• inhibition of pg RNA encapsidation may be effective against HBV and/or HDV variants resistant to the currently available DNA polymerase inhibitors.
• combination therapy of the pg RNA encapsidation inhibitors with DNA polymerase inhibitors may synergistically suppress HBV and/or HDV replication and prevent drug resistance emergence, thus offering a more effective treatment for chronic hepatitis B and/or hepatis D infection.
• novel compounds that can be used to treat and/or prevent HBV and/or HDV infection in a subject.
• the novel compounds inhibit HBV and/or HDV nucleocapsid assembly.
• the novel compounds can be used in patients that are HBV and/or HBV -HDV infected, patients who are at risk of becoming HBV and/or HBV -HDV infected, and/or patients that are infected with drug-resistant HBV and/or HDV.
• the present invention addresses this need.
• the disclosure provides a compound of formula (I) or (II), or a salt, solvate, prodrug, stereoisomer, tautomer, or isotopically labeled derivative thereof, or any mixtures thereof:
• R 3 , R 5 , R 8 , and R 9 are as defined elsewhere herein.
• the disclosure further provides a pharmaceutical composition comprising at least one compound of the disclosure and a pharmaceutically acceptable carrier.
• the disclosure further provides a method of treating or preventing hepatitis B virus (HBV) infection and/or hepatitis D virus (HDV) infection in a subject.
• HBV hepatitis B virus
• HDV hepatitis D virus
• the disclosure further provides a method of inhibiting expression and/or function of a viral capsid protein directly or indirectly in a HBV-infected and/or HDV-infected subject.
• the method of the disclosure comprises administering to the subject in need thereof a therapeutically effective amount of at least one compound of the disclosure.
• the invention relates, in certain aspects, to the discovery of certain substituted bi cyclic compounds that are useful to treat and/or prevent hepatitis B virus (HBV) infection and/or HBV-hepatitis D virus (HDV) infection and related conditions in a subject.
• the compounds of the invention are viral capsid inhibitors.
• an element or component is said to be included in and/or selected from a list of recited elements or components, it should be understood that the element or component can be any one of the recited elements or components and can be selected from a group consisting of two or more of the recited elements or components.
• the acts can be carried out in any order, except when a temporal or operational sequence is explicitly recited. Furthermore, specified acts can be carried out concurrently unless explicit claim language recites that they be carried out separately. For example, a claimed act of doing X and a claimed act of doing Y can be conducted simultaneously within a single operation, and the resulting process will fall within the literal scope of the claimed process.
• the terms“a,”“an,” or“the” are used to include one or more than one unless the context clearly dictates otherwise.
• the term“or” is used to refer to a nonexclusive“or” unless otherwise indicated.
• the statement“at least one of A and B” or“at least one of A or B” has the same meaning as“A, B, or A and B.”
• the term“about” will be understood by persons of ordinary skill in the art and will vary to some extent on the context in which it is used. As used herein,“about” when referring to a measurable value such as an amount, a temporal duration, and the like, is meant to encompass variations of ⁇ 20%, ⁇ 10%, ⁇ 5%, ⁇ 1%, or ⁇ 0.1% from the specified value, as such variations are appropriate to perform the disclosed methods.
• alkenyl employed alone or in combination with other terms, means, unless otherwise stated, a stable monounsaturated or diunsaturated straight chain or branched chain hydrocarbon group having the stated number of carbon atoms.
• Examples include vinyl, propenyl (or allyl), crotyl, isopentenyl, butadienyl, l,3-pentadienyl, l,4-pentadienyl, and the higher homologs and isomers.
• alkoxy employed alone or in combination with other terms means, unless otherwise stated, an alkyl group having the designated number of carbon atoms, as defined elsewhere herein, connected to the rest of the molecule via an oxygen atom, such as, for example, methoxy, ethoxy, l-propoxy, 2-propoxy (or isopropoxy) and the higher homologs and isomers.
• oxygen atom such as, for example, methoxy, ethoxy, l-propoxy, 2-propoxy (or isopropoxy) and the higher homologs and isomers.
• Si-C3alkoxy such as, but not limited to, ethoxy and methoxy.
• alkyl by itself or as part of another substituent means, unless otherwise stated, a straight or branched chain hydrocarbon having the number of carbon atoms designated (i.e., Ci-Cio means one to ten carbon atoms) and includes straight, branched chain, or cyclic substituent groups. Examples include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert- butyl, pentyl, neopentyl, hexyl, and cyclopropylmethyl.
• a specific embodiment is (C
• alkynyl employed alone or in combination with other terms means, unless otherwise stated, a stable straight chain or branched chain hydrocarbon group with a triple carbon-carbon bond, having the stated number of carbon atoms.
• Non- limiting examples include ethynyl and propynyl, and the higher homologs and isomers.
• the term“propargylic” refers to a group exemplified by -CH 2 -CoCH.
• “homopropargylic” refers to a group exemplified by -CH 2 CH 2 -CoCH.
• aromatic refers to a carbocycle or heterocycle with one or more polyunsaturated rings and having aromatic character, i.e., having (4n+2) delocalized p (pi) electrons, where‘n’ is an integer.
• aryl employed alone or in combination with other terms means, unless otherwise stated, a carbocyclic aromatic system containing one or more rings (typically one, two or three rings) wherein such rings may be attached together in a pendent manner, such as a biphenyl, or may be fused, such as naphthalene. Examples include phenyl, anthracyl and naphthyl.
• Aryl groups also include, for example, phenyl or naphthyl rings fused with one or more saturated or partially saturated carbon rings (e.g ., bicyclo[4.2.0]octa-l,3,5- trienyl, or indanyl), which can be substituted at one or more carbon atoms of the aromatic and/or saturated or partially saturated rings.
• phenyl or naphthyl rings fused with one or more saturated or partially saturated carbon rings (e.g ., bicyclo[4.2.0]octa-l,3,5- trienyl, or indanyl), which can be substituted at one or more carbon atoms of the aromatic and/or saturated or partially saturated rings.
• aryl-(Ci-C 6 )alkyl refers to a functional group wherein a one-to-six carbon alkylene chain is attached to an aryl group, e.g., -CH 2 CH 2 -phenyl or -CH 2 - phenyl (or benzyl). Specific examples are aryl-CH 2 - and aryl-CH(CH 3 )-.
• substituted aryl-(Ci-C 6 )alkyl refers to an aryl-(C i-G,)alkyl functional group in which the aryl group is substituted.
• a specific example is substituted aryl(CH 2 )-.
• heteroaryl-(Ci-C 6 )alkyl refers to a functional group wherein a one-to-three carbon alkylene chain is attached to a heteroaryl group, e.g., -CH 2 CH 2 -pyridyl.
• a specific example is heteroaryl-(CH 2 )-.
• substituted heteroaryl-(Ci-C 6 )alkyl refers to a heteroaryl-(Ci- Ce)alkyl functional group in which the heteroaryl group is substituted.
• a specific example is substituted heteroaryl-(CH 2 )-.
• the terms“co-administered” and“co-administration” as relating to a subject refer to administering to the subject a compound and/or composition of the invention along with a compound and/or composition that may also treat or prevent a disease or disorder contemplated herein.
• the co-administered compounds and/or compositions are administered separately, or in any kind of combination as part of a single therapeutic approach.
• the co-administered compound and/or composition may be formulated in any kind of combinations as mixtures of solids and liquids under a variety of solid, gel, and liquid formulations, and as a solution.
• cycloalkyl by itself or as part of another substituent refers to, unless otherwise stated, a cyclic chain hydrocarbon having the number of carbon atoms designated (i.e., G-G, refers to a cyclic group comprising a ring group consisting of three to six carbon atoms) and includes straight, branched chain or cyclic substituent groups.
• Examples of (G-G,)cycloalkyl groups are cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. Cycloalkyl rings can be optionally substituted.
• Non-limiting examples of cycloalkyl groups include: cyclopropyl, 2-methyl-cyclopropyl, cyclopropenyl, cyclobutyl, 2,3-dihydroxycyclobutyl, cyclobutenyl, cyclopentyl, cyclopentenyl, cyclopentadienyl, cyclohexyl, cyclohexenyl, cycloheptyl, cyclooctanyl, decalinyl, 2,5-dimethylcyclopentyl, 3,5- dichlorocyclohexyl, 4-hydroxy cyclohexyl, 3,3,5-trimethylcyclohex-l-yl,
• decahydroazulenyl bicyclo[6.2.0]decanyl, decahydronaphthalenyl, and dodecahydro- 1//- fluorenyl.
• cycloalkyl also includes bicyclic hydrocarbon rings, non-limiting examples of which include, bicyclo[2. l.l]hexanyl, bicyclo[2.2. l]heptanyl,
• a“disease” is a state of health of a subject wherein the subject cannot maintain homeostasis, and wherein if the disease is not ameliorated then the subject’s health continues to deteriorate.
• a“disorder” in a subject is a state of health in which the subject is able to maintain homeostasis, but in which the subject’s state of health is less favorable than it would be in the absence of the disorder. Left untreated, a disorder does not necessarily cause a further decrease in the subject’s state of health.
• halide refers to a halogen atom bearing a negative charge.
• the halide anions are fluoride (F ), chloride (CL), bromide (Br ). and iodide (G).
• halo or“halogen” alone or as part of another substituent refers to, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.
• heteroalkenyl by itself or in combination with another term refers to, unless otherwise stated, a stable straight or branched chain monounsaturated or diunsaturated hydrocarbon group consisting of the stated number of carbon atoms and one or two heteroatoms selected from the group consisting of O, N, and S, and wherein the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen heteroatom may optionally be quatemized. Up to two heteroatoms may be placed consecutively.
• heteroalkyl by itself or in combination with another term refers to, unless otherwise stated, a stable straight or branched chain alkyl group consisting of the stated number of carbon atoms and one or two heteroatoms selected from the group consisting of O, N, and S, and wherein the nitrogen and sulfur atoms may be optionally oxidized and the nitrogen heteroatom may be optionally quatemized.
• the heteroatom(s) may be placed at any position of the heteroalkyl group, including between the rest of the heteroalkyl group and the fragment to which it is attached, as well as attached to the most distal carbon atom in the heteroalkyl group.
• Up to two heteroatoms may be consecutive, such as, for example, -CH 2 NH-OCH 3 , or -CH 2 CH 2 SSCH 3 .
• heteroaryl or“heteroaromatic” refers to a heterocycle having aromatic character.
• a polycyclic heteroaryl may include one or more rings that are partially saturated. Examples include tetrahydroquinoline and 2,3-dihydrobenzofuryl.
• heterocycle or“heterocyclyl” or“heterocyclic” by itself or as part of another substituent refers to, unless otherwise stated, an unsubstituted or substituted, stable, mono- or multi-cyclic heterocyclic ring system that comprises carbon atoms and at least one heteroatom selected from the group consisting of N, O, and S, and wherein the nitrogen and sulfur heteroatoms may be optionally oxidized, and the nitrogen atom may be optionally quatemized.
• the heterocyclic system may be attached, unless otherwise stated, at any heteroatom or carbon atom that affords a stable structure.
• a heterocycle may be aromatic or non-aromatic in nature. In certain embodiments, the heterocycle is a heteroaryl.
• non-aromatic heterocycles include monocyclic groups such as aziridine, oxirane, thiirane, azetidine, oxetane, thietane, pyrrolidine, pyrroline, imidazoline, pyrazolidine, dioxolane, sulfolane, 2,3-dihydrofuran, 2,5-dihydrofuran, tetrahydrofuran, thiophane, piperidine, l,2,3,6-tetrahydropyridine, l,4-dihydropyridine, piperazine, morpholine, thiomorpholine, pyran, 2,3-dihydropyran, tetrahydropyran, l,4-dioxane, 1,3- dioxane, homopiperazine, homopiperidine, l,3-dioxepane, 4,7-dihydro-l,3-dioxepin,
• heteroaryl groups include pyridyl, pyrazinyl, pyrimidinyl (such as, but not limited to, 2- and 4-pyrimidinyl), pyridazinyl, thienyl, furyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, pyrazolyl, isothiazolyl, l,2,3-triazolyl, 1 ,2,4-triazolyl, l,3,4-triazolyl, tetrazolyl, l,2,3-thiadiazolyl, l,2,3-oxadiazolyl, l,3,4-thiadiazolyl, and l,3,4-oxadiazolyl.
• heterocyclyl and heteroaryl moieties are intended to be representative and not limiting.
• composition refers to a mixture of at least one compound useful within the invention with a pharmaceutically acceptable carrier.
• the pharmaceutical composition facilitates administration of the compound to a subject.
• the term“pharmaceutically acceptable” refers to a material, such as a carrier or diluent, which does not abrogate the biological activity or properties of the compound useful within the invention, and is relatively non-toxic, /. e.. the material may be administered to a subject without causing undesirable biological effects or interacting in a deleterious manner with any of the components of the composition in which it is contained.
• the term“pharmaceutically acceptable carrier” means a
• composition or carrier such as a liquid or solid filler, stabilizer, dispersing agent, suspending agent, diluent, excipient, thickening agent, solvent or encapsulating material, involved in carrying or transporting a compound useful within the invention within or to the subject such that it may perform its intended function.
• a pharmaceutically acceptable material, composition or carrier such as a liquid or solid filler, stabilizer, dispersing agent, suspending agent, diluent, excipient, thickening agent, solvent or encapsulating material, involved in carrying or transporting a compound useful within the invention within or to the subject such that it may perform its intended function.
• a pharmaceutically acceptable material, composition or carrier such as a liquid or solid filler, stabilizer, dispersing agent, suspending agent, diluent, excipient, thickening agent, solvent or encapsulating material, involved in carrying or transporting a compound useful within the invention within or to the subject such that it may perform its intended function.
• Such constructs are carried or transported from one
• materials that may serve as pharmaceutically acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as com starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, com oil and soybean oil;
• glycols such as propylene glycol
• polyols such as glycerin, sorbitol, mannitol and polyethylene glycol
• esters such as ethyl oleate and ethyl laurate
• agar buffering agents, such as magnesium hydroxide and aluminum hydroxide; surface-active agents; alginic acid;
• the language“pharmaceutically acceptable salt” refers to a salt of the administered compound prepared from pharmaceutically acceptable non-toxic acids and/or bases, including inorganic acids, inorganic bases, organic acids, inorganic bases, solvates (including hydrates) and clathrates thereof.
• a“pharmaceutically effective amount,”“therapeutically effective amount,” or“effective amount” of a compound is that amount of compound that is sufficient to provide a beneficial effect to the subject to which the compound is administered.
• prevention means avoiding or delaying the onset of symptoms associated with a disease or condition in a subject that has not developed such symptoms at the time the administering of an agent or compound commences.
• Disease, condition and disorder are used interchangeably herein.
• a first molecule preferentially binds to a second molecule (e.g., a particular receptor or enzyme), but does not necessarily bind only to that second molecule.
• a second molecule e.g., a particular receptor or enzyme
• the terms“subject” and“individual” and“patient” can be used interchangeably and may refer to a human or non-human mammal or a bird.
• Non-human mammals include, for example, livestock and pets, such as ovine, bovine, porcine, canine, feline and murine mammals.
• the subject is human.
• substituted refers to that an atom or group of atoms has replaced hydrogen as the substituent attached to another group.
• the term“substituted” as applied to the rings of these groups refers to any level of substitution, namely mono-, di-, tri-, tetra-, or penta-substitution, where such substitution is permitted.
• the substituents are independently selected, and substitution may be at any chemically accessible position. In certain embodiments, the substituents vary in number between one and four. In other embodiments, the substituents vary in number between one and three. In yet another embodiments, the substituents vary in number between one and two.
• the substituents are independently selected from the group consisting of Ci-C 6 alkyl, -OH, Ci-C 6 alkoxy, halo, cyano, amino, acetamido, and nitro.
• the carbon chain may be branched, straight or cyclic.
• the ring when two substituents are taken together to form a ring having a specified number of ring atoms (e.g., R 2 and R 3 taken together with the nitrogen to which they are attached to form a ring having from 3 to 7 ring members), the ring can have carbon atoms and optionally one or more (e.g., 1 to 3) additional heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• the ring can be saturated or partially saturated, and can be optionally substituted.
• substituents of compounds are disclosed in groups or in ranges. It is specifically intended that the description include each and every individual subcombination of the members of such groups and ranges.
• the term“Ci_ 6 alkyl” is specifically intended to individually disclose Ci, C 2 , C 3 , C 4 , C 5 , C 6 , Ci-C 6 , C 1 -C 5 , C 1 -C 4 , C 1 -C 3 , C 1 -C 2 , C 2 -C 6 , C 2 -C 5 , C 2 -C 4 , C 2 -C 3 , C 3 -C 6 , C 3 -C 5 , C 3 -C 4 , C 4 -C 6 , C 4 -C 5 , and C 5 -C 6 alkyl.
• “treat,”“treating” and“treatment,” as used herein, means reducing the frequency or severity with which symptoms of a disease or condition are experienced by a subject by virtue of administering an agent or compound to the subject.
• cccDNA covalently closed circular DNA
• DMSO dimethylsulfoxide
• HBsAg HBV surface antigen
• HBV hepatitis B virus
• HDV hepatitis D virus
• HPLC high performance liquid chromatography
• LCMS liquid chromatography mass spectrometry
• NARTI orNRTI reverse-transcriptase inhibitor
• NMR Nuclear Magnetic Resonance
• NtARTI or NtRTI nucleotide analog reverse-transcriptase inhibitor
• pg RNA pregenomic RNA
• rcDNA relaxed circular DNA
• sAg surface antigen
• TLC thin layer chromatography
• ranges throughout this disclosure, various aspects of the present invention can be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the present invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 2.7, 3, 4, 5, 5.3, and 6.
• a range of“about 0.1% to about 5%” or“about 0.1% to 5%” should be interpreted to include not just about 0.1% to about 5%, but also the individual values (e.g ., 1%, 2%, 3%, and 4%) and the sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the indicated range.
• the statement“about X to Y” has the same meaning as“about X to about Y,” unless indicated otherwise.
• the statement“about X, Y, or about Z” has the same meaning as“about X, about Y, or about Z,” unless indicated otherwise. This applies regardless of the breadth of the range.
• the invention includes a compound of formula (I) or (II), or a salt, solvate, prodrug, isotopically labeled derivative, stereoisomer (such as, in a non-limiting example, an enantiomer or diastereoisomer, and/or any mixtures thereof, such as, in a non-limiting example, mixtures in any proportions of enantiomers and/or diastereoisomers thereof), tautomer and any mixtures thereof, and/or geometric isomer and any mixtures thereof:
• -X 3 -X 2 - is selected from the group consisting of -CH 2 CH 2 -*, -CH 2 CH(CH 3 )-*, - CH 2 C(CH 3 ) 2 -*, -CH(CH 3 )CH 2 -*, -C(CH 3 ) 2 CH 2 -*, -CH(CH 3 )CH(CH 3 )-*, -C(CH 3 ) 2 CH(CH 3 )- *, and -CH(CH 3 )C(CH 3 ) 2 -*, wherein the single bond marked as“*” is formed with X 3 ;
• -X 3 - is selected from the group consisting of a bond (i.e. , absent), -CH 2 -, -CH(CH 3 )-, - C(CH 3 ) 2 -, -CH 2 CH 2 -, *-CH 2 CH(CH 3 )-, *-CH 2 C(CH 3 ) 2 -, *-CH(CH 3 )CH 2 -, *-C(CH 3 ) 2 CH 2 -, - CH(CH 3 )CH(CH 3 )-, *-C(CH 3 ) 2 CH(CH 3 )-*, and *-CH(CH 3 )C(CH 3 ) 2 -, wherein the single bond marked as“*” is formed with X'-X 2 -:
• R 1 is selected from the group consisting of optionally substituted phenyl, optionally substituted benzyl, optionally substituted heteroaryl, and -(CH 2 )(optionally substituted heteroaryl);
• R 3 is H or Ci-C 6 alkyl
• each occurrence of R 4 is independently selected from the group consisting of H and Ci-C 6 alkyl;
• R 5 is selected from the group consisting of H and Ci-C 6 alkyl
• each occurrence of R 6 is independently selected from the group consisting of optionally substituted Ci-C 6 alkyl, optionally substituted Ci-C 6 alkenyl, optionally substituted Ci-C 6 alkynyl, optionally substituted C 3 -C 8 cycloalkyl, optionally substituted phenyl, and optionally substituted hetereoaryl;
• each occurrence of R 6a is independently selected from the group consisting of H, optionally substituted Ci-C 6 alkyl, optionally substituted C 3 -C 8 cycloalkyl, optionally substituted phenyl, and optionally substituted hetereoaryl;
• each occurrence of R 7 is independently selected from the group consisting of H and optionally substituted Ci-C 6 alkyl;
• R 6 and R 7 are bound to the same N atom, R 6 and R 7 optionally combine with the N atom to which both are bound to form an optionally substituted 3-7 membered heterocycle;
• R 8 is selected from the group consisting of H, halo, Ci-C 6 alkyl, Ci-C 6 haloalkyl (such as, but not limited to, trifluoromethyl), C 3 -C 8 cycloalkyl, and optionally substituted phenyl; and
• R 9 is selected from the group consisting of H and Ci-C 6 alkyl.
• the configuration is (R) at the C(R 2 )(R 3 ) center.
• the configuration is ( S) at the C(R 2 )(R 3 ) center.
• the compound of formula (I) is a compound of formula (1-1):
• the compound of formula (I) is a compound of formula (1-2):
• the compound of formula (I) is a compound of formula (1-3):
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (I) is selected from the group consisting of:
• the compound of formula (II) is a compound of formula (II-
• the compound of formula (II) is a compound of formula (II-
• the compound of formula (II) is a compound of formula (II-
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• the compound of formula (II) is selected from the group consisting of:
• each occurrence of aryl or heteroaryl is independently optionally substituted with at least one substituent selected from the group consisting of Ci- Ce alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, heterocyclyl, halo, -CN, -OR c , -N(R C )(R C ), and C 1 -C 6 alkoxy carbonyl, wherein each occurrence of R c is independently H, C 1 -C 6 alkyl or C 3 - Cs cycloalkyl.
• each occurrence of the heteroaryl is independently selected from the group consisting of quinolinyl, imidazo[l,2-a]pyridyl, pyridyl, pyrimidyl, pyrazinyl, imidazolyl, thiazolyl, pyrazolyl, isoxazolyl, oxadiazolyl (including 1,2,3-, 1,2,4-, 1,2,5-, and
• the heteroaryl is quinolinyl.
• the heteroaryl is imidazo[l,2-a]pyridyl.
• the heteroaryl is pyridyl.
• the heteroaryl is pyrimidyl.
• the heteroaryl is pyrazinyl.
• the heteroaryl is imidazolyl.
• the heteroaryl is thiazolyl.
• the heteroaryl is pyrazolyl. In certain embodiments, the heteroaryl is isoxazolyl. In certain embodiments, the heteroaryl is oxadiazolyl. In certain embodiments, the heteroaryl is l,2,3-oxadiazolyl. In certain embodiments, the heteroaryl is l,2,4-oxadiazolyl. In certain embodiments, the heteroaryl is
• the heteroaryl is l,3,4-oxadiazolyl. In certain embodiments, the heteroaryl is tetrazolyl. In certain embodiments, the heteroaryl is triazolyl. In certain embodiments, the heteroaryl is l,2,4-triazolyl. In certain embodiments, the heteroaryl is l,2,3-triazolyl.
• each occurrence of the heterocyclyl group is independently selected from the group consisting of tetrahydrofuranyl, tetrahydropyranyl, piperidinyl, piperazinyl, pyrrolidinyl, morpholinyl, thiomorpholinyl, l-oxido-thiomorpholinyl, 1,1- dioxido-thiomorpholinyl, oxazolidinyl, azetidinyl, and the corresponding oxo analogues (where a methylene ring group is replaced with a carbonyl) thereof, such as but not limited to pyrrolidinonyl, piperidinonyl, piperazinonyl, and/or morpholinonyl.
• the heterocyclyl group is tetrahydrofuranyl. In certain embodiments, the heterocyclyl group is tetrahydropyranyl. In certain embodiments, the heterocyclyl group is piperidinyl. In certain embodiments, the heterocyclyl group is piperazinyl. In certain embodiments, the heterocyclyl group is pyrrolidinyl. In certain embodiments, the heterocyclyl group is morpholinyl. In certain embodiments, the heterocyclyl group is thiomorpholinyl. In certain embodiments, the heterocyclyl group is l-oxido-thiomorpholinyl. In certain embodiments, the heterocyclyl group is l,l-dioxido-thiomorpholinyl.
• the heterocyclyl group is oxazolidinyl. In certain embodiments, the heterocyclyl group is azetidinyl. In certain embodiments, the heterocyclyl group is pyrrolidinonyl. In certain embodiments, the heterocyclyl group is piperidinonyl. In certain embodiments, the heterocyclyl group is piperazinonyl. In certain embodiments, the heterocyclyl group is morpholinonyl.
• R 1 is optionally substituted phenyl. In certain embodiments, R 1 is optionally substituted benzyl. In certain embodiments, R 1 is optionally substituted heteroaryl. In certain embodiments, R 1 is -(CH 2 )(optionally substituted heteroaryl).
• R 1 is selected from the group consisting of optionally substituted phenyl, optionally substituted benzyl, and -(CH 2 )(optionally substituted heteroaryl), wherein the phenyl, benzyl, or heteroaryl is optionally substituted with at least one selected from the group consisting of Ci-C 6 alkyl (such as, for example, methyl, ethyl, and isopropyl), halo (such as, for example, F, Cl, Br, and I), C 1 -C 3 haloalkyl (such as, for example,
• R 1 is selected from the group consisting of: phenyl, 3- chlorophenyl, 4-chlorophenyl, 3-fluorophenyl, 4-fluorophenyl, 3,4-difluorophenyl, 3,5- difluorophenyl, 2,4,5 -trifluorophenyl, 3,4,5-trifluorophenyl, 3,4-dichlorophenyl, 3-chloro-4- fluorophenyl, 4-chloro-3 -fluorophenyl, 4-chloro-3-methylphenyl, 3-chloro-4-methylphenyl, 4-fluoro-3-methylphenyl, 3-fluoro-4-methylphenyl, 4-chloro-3-methoxy phenyl, 3-chloro-4- methoxyphenyl, 4-fluoro-3-methoxyphenyl, 3 -fluoro-4-methoxy phenyl, 3- trifluoromethylphenyl, 4-trifluoromethylphenyl,
• R 1 is 3,4-difluorophenyl. In yet other embodiments, R 1 is 3-chloro-4- fluorophenyl. In yet other embodiments, R 1 is 4-chloro-3-fluorophenyl. In yet other embodiments, R 1 is 3-fluoro-4-methylphenyl. In yet other embodiments, R 1 is 4-fluoro-3- methylphenyl. In yet other embodiments, R 1 is 3-cyano-4-fluorophenyl. In yet other embodiments, R 1 is 3-difluoromethyl-4-fluorophenyl.
• R 4 is H. In certain embodiments, R 4 is Ci-C 6 alkyl. In certain embodiments, each occurrence of R 4 is independently selected from the group consisting of H and methyl. In certain embodiments, R 4 is methyl.
• R 5 is H. In certain embodiments, R 5 is Ci-C 6 alkyl. In certain embodiments, R 5 is H or methyl. In certain embodiments, R 5 is methyl.
• R 6 is optionally substituted Ci-C 6 alkyl. In certain embodiments, R 6 is optionally substituted Ci-C 6 alkenyl. In certain embodiments, R 6 is optionally substituted Ci-C 6 alkynyl. In certain embodiments, R 6 is optionally substituted CN- Cg cycloalkyl. In certain embodiments, R 6 is optionally substituted phenyl. In certain embodiments, R 6 is optionally substituted hetereoaryl. In certain embodiments, R 6 is Ci-C 6 alkyl. In certain embodiments, R 6 is Ci-C 6 alkenyl. In certain embodiments, R 6 is Ci-C 6 alkynyl.
• R 6 is C 3 -C 8 cycloalkyl. In certain embodiments, R 6 is phenyl. In certain embodiments, R 6 is hetereoaryl. In certain embodiments, each occurrence of R 6 is independently selected from the group consisting of Ci-C 6 alkyl optionally substituted with at least one selected from the group consisting of halogen, OH, C 1 -C 3 alkoxy, and cyano; -(CH 2 )o- 3 (optionally substituted heterocyclyl); -(CH 2 )o- 3 (optionally substituted heteroaryl); and -(CH 2 ) 0-3 (optionally substituted heteroaryl).
• R 6a is H. In certain embodiments, R 6a is optionally substituted Ci-C 6 alkyl. In certain embodiments, R 6a is optionally substituted C 3 -C 8 cycloalkyl. In certain embodiments, R 6a is optionally substituted phenyl. In certain embodiments, R 6a is optionally substituted hetereoaryl. In certain embodiments, R 6a is Ci-C 6 alkyl. In certain embodiments, R 6a is C 3 -C 8 cycloalkyl. In certain embodiments, R 6a is phenyl. In certain embodiments, R 6a is hetereoaryl.
• each occurrence of R 6a is independently selected from the group consisting of Ci-C 6 alkyl optionally substituted with at least one selected from the group consisting of halogen, OH, C 1 -C 3 alkoxy, and cyano; - (CH 2 ) 0-3 (optionally substituted heterocyclyl); -(CH 2 ) 0-3 (optionally substituted heteroaryl); and -(CH 2 )o- 3 (optionally substituted heteroaryl).
• R 7 is H. In certain embodiments, R 7 is optionally substituted Ci-C 6 alkyl. In certain embodiments, R 7 is Ci-C 6 alkyl. In certain embodiments, R 7 is H or methyl. In certain embodiments, R 7 is H. In certain embodiments, R 7 is methyl.
• R 6 and R 7 are bound to the same N atom and optionally combine with the N atom to which both are bound to form an optionally substituted 3-7 membered heterocycle.
• R 8 is H. In certain embodiments, R 8 is halo. In certain embodiments, R 8 is Ci-C 6 alkyl. In certain embodiments, R 8 is Ci-C 6 haloalkyl. In certain embodiments, R 8 is trifluoromethyl. In certain embodiments, R 8 is C 3 -C 8 cycloalkyl. In certain embodiments, R 8 is optionally substituted phenyl. In certain embodiments, R 8 is phenyl.
• R 8 is selected from the group consisting of H, halo, and methyl. In certain embodiments, R 8 is H. In certain embodiments, R 8 is methyl. In certain embodiments, R 8 is fluoro. In certain embodiments, R 8 is chloro. In certain embodiments, R 8 is bromo. In certain embodiments, R 8 is iodo.
• R 9 is H. In certain embodiments, R 9 is Ci-C 6 alkyl. In certain embodiments, R 9 is selected from the group consisting of H and methyl. In certain embodiments, R 9 is H. In certain embodiments, R 9 is methyl.
• -X'-X 2 - is -CH 2 CH 2 -*. In certain embodiments, -X'-X 2 - is - CH 2 CH(CH 3 )-*. In certain embodiments, -X'-X 2 - is -CH 2 C(CH 3 ) 2 -*. In certain embodiments, -X 1_ X 2 - is -CH(CH 3 )CH 2 -*. In certain embodiments, -X'-X 2 - is -C(CH 3 ) 2 CH 2 -*. In certain embodiments, -X'-X 2 - is -CH(CH 3 )CH(CH 3 )-*.
• -X'-X 2 - is - C(CH 3 ) 2 CH(CH 3 )-*. In certain embodiments, -X'-X 2 - is -CH(CH 3 )C(CH 3 ) 2 -*. As defined elsewhere herein, the single bond marked as“*” is formed with X 3 .
• -X 3 - is a bond (i.e., absent). In certain embodiments, -X 3 - is - CH 2 -. In certain embodiments, -X 3 - is -CH(CH 3 )-. In certain embodiments, -X 3 - is -C(CH 3 ) 2 -. In certain embodiments, -X 3 - is -CH 2 CH 2 -. In certain embodiments, -X 3 - is *-CH 2 CH(CH 3 )-. In certain embodiments, -X 3 - is *-CH 2 C(CH 3 ) 2 -. In certain embodiments, -X 3 - is *- CH(CH 3 )CH 2 -.
• -X 3 - is *-C(CH 3 ) 2 CH 2 -. In certain embodiments, -X 3 - is -CH(CH 3 )CH(CH 3 )-. In certain embodiments, -X 3 - is *-C(CH 3 ) 2 CH(CH 3 )-*. In certain embodiments, -X 3 - is *-CH(CH 3 )C(CH 3 ) 2 -. As defined elsewhere herein, the single bond marked as“*” is formed with X'-X 2 -:
• the compound of the invention is any compound disclosed herein, or a salt, solvate, prodrug, isotopically labeled (such as for example at least partially deuterated), stereoisomer, any mixture of stereoisomers, tautomer, and/or any mixture of tautomers thereof.
• the compound is at least one selected from Table 1, or a salt, solvate, prodrug, isotopically labeled, stereoisomer, any mixture of stereoisomers, tautomer, and/or any mixture of tautomers thereof.
• the compounds of the invention may possess one or more stereocenters, and each stereocenter may exist independently in either the ( R ) or (S) configuration.
• compounds described herein are present in optically active or racemic forms.
• the compounds described herein encompass racemic, optically active, regioisomeric and stereoisomeric forms, or combinations thereof that possess the therapeutically useful properties described herein. Preparation of optically active forms is achieved in any suitable manner, including, by way of non-limiting example, by resolution of the racemic form with recrystallization techniques, synthesis from optically active starting materials, chiral synthesis, or chromatographic separation using a chiral stationary phase.
• a compound illustrated herein by the racemic formula further represents either of the two enantiomers or any mixtures thereof, or in the case where two or more chiral centers are present, all diastereomers or any mixtures thereof.
• the compounds of the invention exist as tautomers. All tautomers are included within the scope of the compounds recited herein.
• Compounds described herein also include isotopically labeled compounds wherein one or more atoms is replaced by an atom having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature.
• isotopes suitable for inclusion in the compounds described herein include and are not limited to 2 H, 3 ⁇ 4 n C, 13 C, 14 C, 36 Cl, 18 F, 123 I, 125 I, 13 N, 15 N, 15 0, 17 0, 18 0, 32 P, and 35 S. In certain embodiments, substitution with heavier isotopes such as deuterium affords greater chemical stability.
• Isotopically labeled compounds are prepared by any suitable method or by processes using an appropriate isotopically labeled reagent in place of the non-labeled reagent otherwise employed.
• the compounds described herein are labeled by other means, including, but not limited to, the use of chromophores or fluorescent moieties, bioluminescent labels, or chemiluminescent labels.
• the compounds described herein may form salts with acids or bases, and such salts are included in the present invention.
• the term“salts” embraces addition salts of free acids or bases that are useful within the methods of the invention.
• pharmaceutically acceptable salt refers to salts that possess toxicity profiles within a range that affords utility in pharmaceutical applications.
• the salts are pharmaceutically acceptable salts.
• Pharmaceutically unacceptable salts may nonetheless possess properties such as high crystallinity, which have utility in the practice of the present invention, such as for example utility in process of synthesis, purification or formulation of compounds useful within the methods of the invention.
• Suitable pharmaceutically acceptable acid addition salts may be prepared from an inorganic acid or from an organic acid.
• inorganic acids include sulfate, hydrogen sulfate, hydrochloric, hydrobromic, hydriodic, nitric, carbonic, sulfuric, and phosphoric acids (including hydrogen phosphate and dihydrogen phosphate).
• organic acids may be selected from aliphatic, cycloaliphatic, aromatic, araliphatic, heterocyclic, carboxylic and sulfonic classes of organic acids, examples of which include formic, acetic, propionic, succinic, glycolic, gluconic, lactic, malic, tartaric, citric, ascorbic, glucuronic, maleic, fumaric, pyruvic, aspartic, glutamic, benzoic, anthranilic, 4-hydroxybenzoic, phenylacetic, mandelic, embonic (or pamoic), methanesulfonic, ethanesulfonic, benzenesulfonic, pantothenic, sulfanilic, 2-hydroxyethanesulfonic, trifluoromethanesulfonic, p-toluenesulfonic, cyclohexylaminosulfonic, stearic, alginic, b-hydroxybutyric, sal
• Suitable pharmaceutically acceptable base addition salts of compounds of the invention include, for example, ammonium salts and metallic salts including alkali metal, alkaline earth metal and transition metal salts such as, for example, calcium, magnesium, potassium, sodium and zinc salts.
• Pharmaceutically acceptable base addition salts also include organic salts made from basic amines such as, for example, W -diben/ylethylene- diamine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumine (or N- methylglucamine) and procaine. All of these salts may be prepared from the corresponding compound by reacting, for example, the appropriate acid or base with the compound.
• the compounds of the invention are useful within the methods of the invention in combination with one or more additional agents useful for treating HBV and/or HDV infections.
• additional agents may comprise compounds or compositions identified herein, or compounds (e.g., commercially available compounds) known to treat, prevent, or reduce the symptoms of HBV and/or HDV infections.
• Non-limiting examples of one or more additional agents useful for treating HBV infections include: (a) reverse transcriptase inhibitors; (b) capsid inhibitors; (c) cccDNA formation inhibitors; (d) RNA destabilizers; (e) oligomeric nucleotides targeted against the HBV genome; (f) immunostimulators, such as checkpoint inhibitors (e.g., PD-L1 inhibitors); and (g) GalNAc-siRNA conjugates targeted against an HBV gene transcript.
• the reverse transcriptase inhibitor is a reverse-transcriptase inhibitor (NARTI or NRTI). In other embodiments, the reverse transcriptase inhibitor is a nucleotide analog reverse-transcriptase inhibitor (NtARTI or NtRTI).
• Reported reverse transcriptase inhibitors include, but are not limited to, entecavir, clevudine, telbivudine, lamivudine, adefovir, and tenofovir, tenofovir disoproxil, tenofovir alafenamide, adefovir dipovoxil, (li?,2i?,3i?,5i?)-3-(6-amino-9i/-9-purinyl)-2-fluoro-5- (hydroxymethyl)-4-methylenecyclopentan-l-ol (described in U.S. Patent No.
• Reported reverse transcriptase inhibitors further include, but are not limited to, entecavir, lamivudine, and (li?,2i?,3i?,5i?)-3-(6-amino-9i/-9-purinyl)-2-fluoro-5- (hy droxymethyl)-4-methylenecy clopentan- 1 -ol.
• Reported reverse transcriptase inhibitors further include, but are not limited to, a covalently bound phosphoramidate or phosphonamidate moiety of the above-mentioned reverse transcriptase inhibitors, or as described in for example U.S. Patent No. 8,816,074, US Patent Application Publications No. US 2011/0245484 Al, and US 2008/0286230A1, all of which incorporated herein in their entireties by reference.
• Reported reverse transcriptase inhibitors further include, but are not limited to, nucleotide analogs that comprise a phosphoramidate moiety, such as, for example, methyl (((( 1 //.3//.4//.5//)-3-(6-amino-9//-purin-9-yl)-4-nuoro-5-hydroxy-2-methylenecyclopentyl) methoxyXphenoxy) phosphoryl)-(D or L)-alaninate and methyl ((((li?,2i?,3i?,4i?)-3-fluoro-2- hydro ⁇ y-5-methylene-4-(6-o ⁇ o- 1.6-dihydro-9//-purin-9-yl)cyclopentyl)methoxy)(phenoxy) phosphoryl)-(D or L)-alaninate.
• nucleotide analogs that comprise a phosphoramidate moiety, such as, for example, methyl (((( 1 //.3//.4
• the individual diastereomers thereof which include, for example, methyl ((R)-((( l//.3//.4//.5//)-3-(6-amino-9//-purin-9-yl)-4-nuoro-5- hydroxy-2-methylenecyclopentyl)methoxy)(phenoxy)phosphoryl)-(D or L)-alaninate and methyl (( ⁇ S)-(((li?,3i?,4i?,5i?)-3-(6-amino-9i/-purin-9-yl)-4-fluoro-5-hydroxy-2- methylenecyclopentyl) methoxyXphenoxy )phosphoryl)-(D or L)-alaninate.
• Reported reverse transcriptase inhibitors further include, but are not limited to, compounds comprising a phosphonamidate moiety, such as, for example, tenofovir alafenamide, as well as those described in U.S. Patent Application Publication No. US 2008/0286230 Al, incorporated herein in its entirety by reference.
• Methods for preparing stereoselective phosphoramidate or phosphonamidate containing actives are described in, for example, U.S. Patent No. 8,816,074, as well as U.S. Patent Application Publications No. US 2011/0245484 Al and US 2008/0286230 Al, all of which incorporated herein in their entireties by reference.
• capsid inhibitor includes compounds that are capable of inhibiting the expression and/or function of a capsid protein either directly or indirectly.
• a capsid inhibitor may include, but is not limited to, any compound that inhibits capsid assembly, induces formation of non-capsid polymers, promotes excess capsid assembly or misdirected capsid assembly, affects capsid stabilization, and/or inhibits encapsidation of RNA (pgRNA).
• Capsid inhibitors also include any compound that inhibits capsid function in a downstream event(s) within the replication process (e.g., viral DNA synthesis, transport of relaxed circular DNA (rcDNA) into the nucleus, covalently closed circular DNA (cccDNA) formation, virus maturation, budding and/or release, and the like).
• the inhibitor detectably inhibits the expression level or biological activity of the capsid protein as measured, e.g., using an assay described herein.
• the inhibitor inhibits the level of rcDNA and downstream products of viral life cycle by at least 5%, at least 10%, at least 20%, at least 50%, at least 75%, or at least 90%.
• Reported capsid inhibitors include, but are not limited to, compounds described in International Patent Applications Publication Nos WO 2013006394, WO 2014106019, and WO2014089296, all of which incorporated herein in their entireties by reference.
• Reported capsid inhibitors also include, but are not limited to, the following compounds and pharmaceutically acceptable salts and/or solvates thereof: Bay -41-4109 (see Int’l Patent Application Publication No. WO 2013144129), AT-61 (see Int’l Patent
• capsid inhibitors include, but are not limited to, those generally and specifically described in U.S. Patent Application Publication Nos. US 2015/0225355, US 2015/0132258, US 2016/0083383, US 2016/0052921 and Int’l Patent Application Publication Nos. WO 2013096744, WO 2014165128, WO 2014033170, WO 2014033167, WO 2014033176, WO 2014131847, WO 2014161888, WO 2014184350, WO 2014184365, WO 2015059212, WO 2015011281, WO 2015118057, WO 2015109130, WO 2015073774,
• WO 2015180631 WO 2015138895, WO 2016089990, WO 2017015451, WO 2016183266, WO 2017011552, WO 2017048950, WO2017048954, WO 2017048962, WO 2017064156 and are incorporated herein in their entirety by reference.
• cccDNA Covalently closed circular DNA
• cccDNA Covalently closed circular DNA
• cccDNA formation inhibitor includes compounds that are capable of inhibiting the formation and/or stability of cccDNA either directly or indirectly.
• a cccDNA formation inhibitor may include, but is not limited to, any compound that inhibits capsid disassembly, rcDNA entry into the nucleus, and/or the conversion of rcDNA into cccDNA.
• the inhibitor detectably inhibits the formation and/or stability of the cccDNA as measured, e.g., using an assay described herein.
• the inhibitor inhibits the formation and/or stability of cccDNA by at least 5%, at least 10%, at least 20%, at least 50%, at least 75%, or at least 90%.
• Reported cccDNA formation inhibitors include, but are not limited to, compounds described in Int’l Patent Application Publication No. WO 2013130703, and are incorporated herein in their entirety by reference.
• cccDNA formation inhibitors include, but are not limited to, those generally and specifically described in U.S. Patent Application Publication No. US 2015/0038515 Al, and are incorporated herein in their entirety by reference.
• RNA destabilizer refers to a molecule, or a salt or solvate thereof, that reduces the total amount of HBV RNA in mammalian cell culture or in a live human subject.
• an RNA destabilizer reduces the amount of the RNA transcript(s) encoding one or more of the following HBV proteins: surface antigen, core protein, RNA polymerase, and e antigen.
• the RNA destabilizer reduces the total amount of HBV RNA in mammalian cell culture or in a live human subject by at least 5%, at least 10%, at least 20%, at least 50%, at least 75%, or at least 90%.
• Reported RNA destabilizers include compounds described in U.S. Patent No.
• RNA destabilizers include, but are not limited to, those generally and specifically described in Int’l Patent Application Publication Nos.
• Reported oligomeric nucleotides targeted against the HBV genome include, but are not limited to, Arrowhead- ARC-520 (see U.S. Patent No. 8,809,293; and Wooddell et al, 2013, Molecular Therapy 2l(5):973-985, all of which incorporated herein in their entireties by reference).
• the oligomeric nucleotides can be designed to target one or more genes and/or transcripts of the HBV genome.
• Oligomeric nucleotide targeted to the HBV genome also include, but are not limited to, isolated, double stranded, siRNA molecules, that each include a sense strand and an antisense strand that is hybridized to the sense strand.
• the siRNA target one or more genes and/or transcripts of the HBV genome.
• checkpoint inhibitor includes any compound that is capable of inhibiting immune checkpoint molecules that are regulators of the immune system (e.g., stimulate or inhibit immune system activity).
• some checkpoint inhibitors block inhibitory checkpoint molecules, thereby stimulating immune system function, such as stimulation of T cell activity against cancer cells.
• a non-limting example of a checkpoint inhibitor is a PD-Ll inhibitor.
• the term“PD-L1 inhibitor” includes any compound that is capable of inhibiting the expression and/or function of the protein Programmed Death-Ligand 1 (PD-L1) either directly or indirectly.
• PD-L1 also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a type 1 transmembrane protein that plays a major role in suppressing the adaptive arm of immune system during pregnancy, tissue allograft transplants, autoimmune disease, and hepatitis.
• PD-L1 binds to its receptor, the inhibitory checkpoint molecule PD-l (which is found on activated T cells, B cells, and myeloid cells) so as to modulate activation or inhibition of the adaptive arm of immune system.
• the PD-L1 inhibitor inhibits the expression and/or function of PD-L1 by at least 5%, at least 10%, at least 20%, at least 50%, at least 75%, or at least 90%.
• Reported PD-L1 Inhibitors include, but are not limited to, compounds recited in one of the following patent application publications: US 2018/0057455; US 2018/0057486; WO 2017/106634; WO 2018/026971; WO 2018/045142; WO 2018/118848; WO 2018/119221; WO 2018/119236; WO 2018/119266; WO 2018/119286; WO 2018/121560; WO
• GalNAc-siRNA Conjugates Targeted against an HBV Gene Transcript “GalNAc” is the abbreviation for N-acetylgalactosamine, and“siRNA” is the abbreviation for small interfering RNA.
• An siRNA that targets an HBV gene transcript is covalently bonded to GalNAc in a GalNAc-siRNA conjugate useful in the practice of the present invention. While not wishing to be bound by theory, it is believed that GalNAc binds to asialoglycoprotein receptors on hepatocytes thereby facilitating the targeting of the siRNA to the hepatocytes that are infected with HBV. The siRNA enter the infected hepatocytes and stimulate destruction of HBV gene transcripts by the phenomenon of RNA interference.
• GalNAc-siRNA conjugates useful in the practice of this aspect of the present invention are set forth in published international application PCT/CA2017/050447 (PCT Application Publication number WO/2017/177326, published on October 19, 2017) which is hereby incorporated by reference in its entirety.
• a synergistic effect may be calculated, for example, using suitable methods such as, for example, the Sigmoid-E max equation (Holford & Scheiner, 1981, Clin. Pharmacokinet. 6:429-453), the equation of Loewe additivity (Loewe & Muischnek, 1926, Arch. Exp. Pathol Pharmacol. 114: 313-326) and the median-effect equation (Chou & Talalay, 1984, Adv. Enzyme Regul. 22:27-55).
• Each equation referred to elsewhere herein may be applied to experimental data to generate a corresponding graph to aid in assessing the effects of the drug combination.
• the corresponding graphs associated with the equations referred to elsewhere herein are the concentration-effect curve, isobologram curve and combination index curve, respectively.
• the present invention further provides methods of preparing compounds of the present invention.
• Compounds of the present teachings can be prepared in accordance with the procedures outlined herein, from commercially available starting materials, compounds known in the literature, or readily prepared intermediates, by employing standard synthetic methods and procedures known to those skilled in the art. Standard synthetic methods and procedures for the preparation of organic molecules and functional group transformations and manipulations can be readily obtained from the relevant scientific literature or from standard textbooks in the field.
• reaction temperatures i.e.. reaction temperatures, times, mole ratios of reactants, solvents, pressures, and so forth
• Optimum reaction conditions can vary with the particular reactants or solvent used, but such conditions can be determined by one skilled in the art by routine optimization procedures.
• Those skilled in the art of organic synthesis will recognize that the nature and order of the synthetic steps presented can be varied for the purpose of optimizing the formation of the compounds described herein.
• product formation can be monitored by spectroscopic means, such as nuclear magnetic resonance spectroscopy (e.g., 'H or 13 C), infrared spectroscopy, spectrophotometry (e.g., UV-visible), mass spectrometry, or by chromatography such as high-performance liquid chromatograpy (HPLC), gas chromatography (GC), gel-permeation chromatography (GPC), or thin layer chromatography (TLC).
• spectroscopic means such as nuclear magnetic resonance spectroscopy (e.g., 'H or 13 C), infrared spectroscopy, spectrophotometry (e.g., UV-visible), mass spectrometry, or by chromatography such as high-performance liquid chromatograpy (HPLC), gas chromatography (GC), gel-permeation chromatography (GPC), or thin layer chromatography (TLC).
• HPLC high-performance liquid chromatograpy
• GC gas chromatography
• GPC gel-per
• Preparation of the compounds can involve protection and deprotection of various chemical groups.
• the need for protection and deprotection and the selection of appropriate protecting groups can be readily determined by one skilled in the art.
• the chemistry of protecting groups can be found, for example, in Greene, et al. , Protective Groups in Organic Synthesis, 2d. Ed. (Wiley & Sons, 1991), the entire disclosure of which is incorporated by reference herein for all purposes.
• Suitable solvents typically are substantially nonreactive with the reactants, intermediates, and/or products at the temperatures at which the reactions are carried out, i.e., temperatures that can range from the solvent’s freezing temperature to the solvent’s boiling temperature.
• a given reaction can be carried out in one solvent or a mixture of more than one solvent.
• suitable solvents for a particular reaction step can be selected.
• a compound of formula (I) can be prepared, for example, according to the synthetic methods outlined in Scheme 1 :
• Conversion of a cyclic a,b-unsaturated ketone to bicyclic pyrrole derivative III can be achieved by reaction with tosylmethyl isocyanide (TosMIC) (exemplified in Tetrahedron Lett., 2012, 53, 819).
• TosMIC tosylmethyl isocyanide
• Bromination of III with V-bromosucci n i mi de to provide IV can be followed by V-functionalization to generate V.
• Palladium (II) catalyzed carbonylation in the presence of carbon monoxide and ethanol provides ester VI, which can be hydrolyzed and converted to carboxamide VIII.
• Reductive amination either by chiral or racemic methods, utilizing ketone VIII can be performed to provide IX, which can be further functionalized to provide X.
• the invention provides a method of treating or preventing hepatitis virus infection in a subject.
• the infection comprises hepatitis B virus (HBV) infection.
• the infection comprises hepatitis D virus (HDV) infection.
• the infection comprises HBV -HDV infection.
• the method comprises administering to the subject in need thereof a therapeutically effective amount of at least one compound of the invention.
• the at least one compound of the invention is the only antiviral agent administered to the subject.
• the at least one compound is administered to the subject in a pharmaceutically acceptable composition.
• the subject is further administered at least one additional agent useful for treating the hepatitis infection.
• the at least one additional agent comprises at least one selected from the group consisting of reverse transcriptase inhibitor, capsid inhibitor, cccDNA formation inhibitor, RNA destabilizer, oligomeric nucleotide targeted against the HBV genome, immunostimulator, and GalNAc-siRNA conjugate targeted against an HBV gene transcript.
• the at least one additional agent comprises at least one selected from the group consisting of reverse transcriptase inhibitor, capsid inhibitor, cccDNA formation inhibitor, RNA destabilizer, oligomeric nucleotide targeted against the HBV genome, immunostimulator, and GalNAc-siRNA conjugate targeted against an HBV gene transcript.
• the subject is co-administered the at least one compound and the at least one additional agent.
• the at least one compound and the at least one additional agent are coformulated.
• the invention further provides a method of inhibiting expression and/or function of a viral capsid protein either directly or indirectly in a subject.
• the method comprises administering to the subject in need thereof a therapeutically effective amount of at least one compound of the invention.
• the at least one compound is administered to the subject in a pharmaceutically acceptable composition.
• the at least one compound of the invention is the only antiviral agent administered to the subject.
• the subject is further administered at least one additional agent useful for treating HBV and/or HDV infection.
• the at least one additional agent comprises at least one selected from the group consisting of reverse transcriptase inhibitor, capsid inhibitor, cccDNA formation inhibitor, RNA destabilizer, oligomeric nucleotide targeted against the HBV genome,
• the subject is co-administered the at least one compound and the at least one additional agent.
• the at least one compound and the at least one additional agent are coformulated.
• the subject is a mammal. In yet other embodiments, the mammal is a human.
• the invention further provides methods of preparing compounds of the invention, using for examples synthetic transformations illustrated in Schemes 1-4, or any experimental examples recited herein.
• the invention provides pharmaceutical compositions comprising at least one compound of the invention or a salt or solvate thereof, which are useful to practice methods of the invention.
• a pharmaceutical composition may consist of at least one compound of the invention or a salt or solvate thereof, in a form suitable for administration to a subject, or the pharmaceutical composition may comprise at least one compound of the invention or a salt or solvate thereof, and one or more pharmaceutically acceptable carriers, one or more additional ingredients, or any combinations of these.
• At least one compound of the invention may be present in the pharmaceutical composition in the form of a physiologically acceptable salt, such as in combination with a physiologically acceptable cation or anion, as is well known in the art.
• the pharmaceutical compositions useful for practicing the method of the invention may be administered to deliver a dose of between 1 ng/kg/day and 100 mg/kg/day. In other embodiments, the pharmaceutical compositions useful for practicing the invention may be administered to deliver a dose of between 1 ng/kg/day and 1,000 mg/kg/day.
• compositions of the invention will vary, depending upon the identity, size, and condition of the subject treated and further depending upon the route by which the composition is to be administered.
• the composition may comprise between 0.1% and 100% (w/w) active ingredient.
• compositions that are useful in the methods of the invention may be suitably developed for nasal, inhalational, oral, rectal, vaginal, pleural, peritoneal, parenteral, topical, transdermal, pulmonary, intranasal, buccal, ophthalmic, epidural, intrathecal, intravenous, or another route of administration.
• a composition useful within the methods of the invention may be directly administered to the brain, the brainstem, or any other part of the central nervous system of a mammal or bird.
• Other contemplated formulations include projected nanoparticles, microspheres, liposomal preparations, coated particles, polymer conjugates, resealed erythrocytes containing the active ingredient, and immunologically- based formulations.
• compositions of the invention are part of a
• pharmaceutical matrix may be prepared using hot melt extrusion, solid solutions, solid dispersions, size reduction technologies, molecular complexes (e.g., cyclodextrins, and others), microparticulate, and particle and formulation coating processes.
• Amorphous or crystalline phases may be used in such processes.
• the route(s) of administration will be readily apparent to the skilled artisan and will depend upon any number of factors including the type and severity of the disease being treated, the type and age of the veterinary or human patient being treated, and the like.
• compositions described herein may be prepared by any method known or hereafter developed in the art of pharmacology and pharmaceutics.
• preparatory methods include the step of bringing the active ingredient into association with a carrier or one or more other accessory ingredients, and then, if necessary or desirable, shaping or packaging the product into a desired single-dose or multi-dose unit.
• a“unit dose” is a discrete amount of the pharmaceutical composition comprising a predetermined amount of the active ingredient.
• the amount of the active ingredient is generally equal to the dosage of the active ingredient that would be administered to a subject or a convenient fraction of such a dosage such as, for example, one-half or one- third of such a dosage.
• the unit dosage form may be for a single daily dose or one of multiple daily doses (e.g., about 1 to 4 or more times per day). When multiple daily doses are used, the unit dosage form may be the same or different for each dose.
• compositions suitable for ethical administration to humans are principally directed to pharmaceutical compositions suitable for ethical administration to humans, it will be understood by the skilled artisan that such compositions are generally suitable for administration to animals of all sorts. Modification of pharmaceutical compositions suitable for administration to humans in order to render the compositions suitable for administration to various animals is well understood, and the ordinarily skilled veterinary pharmacologist can design and perform such modification with merely ordinary, if any, experimentation. Subjects to which administration of the pharmaceutical compositions of the invention is contemplated include, but are not limited to, humans and other primates, mammals including commercially relevant mammals such as cattle, pigs, horses, sheep, cats, and dogs.
• compositions of the invention are formulated using one or more pharmaceutically acceptable excipients or carriers.
• pharmaceutical compositions of the invention comprise a therapeutically effective amount of at least one compound of the invention and a pharmaceutically acceptable carrier.
• Pharmaceutically acceptable carriers include, but are not limited to, glycerol, water, saline, ethanol, recombinant human albumin (e.g., RECOMBUMIN®), solubilized gelatins (e.g., GELOFUSINE®), and other pharmaceutically acceptable salt solutions such as phosphates and salts of organic acids. Examples of these and other pharmaceutically acceptable carriers are described in Remington’s Pharmaceutical Sciences (1991, Mack Publication Co., New Jersey).
• the carrier may be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), recombinant human albumin, solubilized gelatins, suitable mixtures thereof, and vegetable oils.
• the proper fluidity may be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
• Prevention of the action of microorganisms may be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like.
• isotonic agents for example, sugars, sodium chloride, or poly alcohols such as mannitol and sorbitol, are included in the composition.
• Prolonged absorption of the injectable compositions may be brought about by including in the composition an agent that delays absorption, for example, aluminum monostearate or gelatin.
• Formulations may be employed in admixtures with conventional excipients, /. e.. pharmaceutically acceptable organic or inorganic carrier substances suitable for oral, parenteral, nasal, inhalational, intravenous, subcutaneous, transdermal enteral, or any other suitable mode of administration, known to the art.
• the pharmaceutical preparations may be sterilized and if desired mixed with auxiliary agents, e.g., lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure buffers, coloring, flavoring, and/or fragrance-conferring substances and the like.
• ingredients include, but are not limited to, one or more ingredients that may be used as a pharmaceutical carrier.
• the composition of the invention may comprise a preservative from about 0.005% to 2.0% by total weight of the composition.
• the preservative is used to prevent spoilage in the case of exposure to contaminants in the environment.
• Examples of preservatives useful in accordance with the invention include but are not limited to those selected from the group consisting of benzyl alcohol, sorbic acid, parabens, imidurea and any combinations thereof.
• One such preservative is a combination of about 0.5% to 2.0% benzyl alcohol and 0.05-0.5% sorbic acid.
• the composition may include an antioxidant and a chelating agent that inhibit the degradation of the compound.
• Antioxidants for some compounds are BHT, BHA, alpha- tocopherol and ascorbic acid in the exemplary range of about 0.01% to 0.3%, or BHT in the range of 0.03% to 0.1% by weight by total weight of the composition.
• the chelating agent may be present in an amount of from 0.01% to 0.5% by weight by total weight of the composition.
• Exemplary chelating agents include edetate salts (e.g. disodium edetate) and citric acid in the weight range of about 0.01% to 0.20%, or in the range of 0.02% to 0.10% by weight by total weight of the composition.
• the chelating agent is useful for chelating metal ions in the composition that may be detrimental to the shelf life of the formulation. While BHT and disodium edetate are exemplary antioxidant and chelating agent, respectively, for some compounds, other suitable and equivalent antioxidants and chelating agents may be substituted therefore as would be known to those skilled in the art.
• Liquid suspensions may be prepared using conventional methods to achieve suspension of the active ingredient in an aqueous or oily vehicle.
• Aqueous vehicles include, for example, water, and isotonic saline.
• Oily vehicles include, for example, almond oil, oily esters, ethyl alcohol, vegetable oils such as arachis, olive, sesame, or coconut oil, fractionated vegetable oils, and mineral oils such as liquid paraffin.
• Liquid suspensions may further comprise one or more additional ingredients including, but not limited to, suspending agents, dispersing or wetting agents, emulsifying agents, demulcents, preservatives, buffers, salts, flavorings, coloring agents, and sweetening agents.
• Oily suspensions may further comprise a thickening agent.
• suspending agents include, but are not limited to, sorbitol syrup, hydrogenated edible fats, sodium alginate, polyvinylpyrrolidone, gum tragacanth, gum acacia, and cellulose derivatives such as sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethyl cellulose.
• Known dispersing or wetting agents include, but are not limited to, naturally-occurring phosphatides such as lecithin, condensation products of an alkylene oxide with a fatty acid, with a long chain aliphatic alcohol, with a partial ester derived from a fatty acid and a hexitol, or with a partial ester derived from a fatty acid and a hexitol anhydride (e.g ., polyoxyethylene stearate, heptadecaethyleneoxycetanol,
• polyoxyethylene sorbitol monooleate and polyoxyethylene sorbitan monooleate, respectively).
• emulsifying agents include, but are not limited to, lecithin, acacia, and ionic or non-ionic surfactants.
• preservatives include, but are not limited to, methyl, ethyl, or «-propyl para-hydroxybenzoates, ascorbic acid, and sorbic acid.
• Known sweetening agents include, for example, glycerol, propylene glycol, sorbitol, sucrose, and saccharin.
• Liquid solutions of the active ingredient in aqueous or oily solvents may be prepared in substantially the same manner as liquid suspensions, the primary difference being that the active ingredient is dissolved, rather than suspended in the solvent.
• an“oily” liquid is one which comprises a carbon-containing liquid molecule and which exhibits a less polar character than water.
• Liquid solutions of the pharmaceutical composition of the invention may comprise each of the components described with regard to liquid suspensions, it being understood that suspending agents will not necessarily aid dissolution of the active ingredient in the solvent.
• Aqueous solvents include, for example, water, and isotonic saline.
• Oily solvents include, for example, almond oil, oily esters, ethyl alcohol, vegetable oils such as arachis, olive, sesame, or coconut oil, fractionated vegetable oils, and mineral oils such as liquid paraffin.
• Powdered and granular formulations of a pharmaceutical preparation of the invention may be prepared using known methods. Such formulations may be administered directly to a subject, used, for example, to form tablets, to fill capsules, or to prepare an aqueous or oily suspension or solution by addition of an aqueous or oily vehicle thereto. Each of these formulations may further comprise one or more of dispersing or wetting agent, a suspending agent, ionic and non-ionic surfactants, and a preservative. Additional excipients, such as fillers and sweetening, flavoring, or coloring agents, may also be included in these formulations.
• a pharmaceutical composition of the invention may also be prepared, packaged, or sold in the form of oil-in-water emulsion or a water-in-oil emulsion.
• the oily phase may be a vegetable oil such as olive or arachis oil, a mineral oil such as liquid paraffin, or a combination of these.
• compositions may further comprise one or more emulsifying agents such as naturally occurring gums such as gum acacia or gum tragacanth, naturally- occurring phosphatides such as soybean or lecithin phosphatide, esters or partial esters derived from combinations of fatty acids and hexitol anhydrides such as sorbitan monooleate, and condensation products of such partial esters with ethylene oxide such as polyoxyethylene sorbitan monooleate.
• emulsions may also contain additional ingredients including, for example, sweetening or flavoring agents.
• Methods for impregnating or coating a material with a chemical composition include, but are not limited to methods of depositing or binding a chemical composition onto a surface, methods of incorporating a chemical composition into the structure of a material during the synthesis of the material (i.e.. such as with a
• physiologically degradable material and methods of absorbing an aqueous or oily solution or suspension into an absorbent material, with or without subsequent drying.
• Methods for mixing components include physical milling, the use of pellets in solid and suspension formulations and mixing in a transdermal patch, as known to those skilled in the art.
• the regimen of administration may affect what constitutes an effective amount.
• the therapeutic formulations may be administered to the patient either prior to or after the onset of a disease or disorder. Further, several divided dosages, as well as staggered dosages may be administered daily or sequentially, or the dose may be continuously infused, or may be a bolus injection. Further, the dosages of the therapeutic formulations may be proportionally increased or decreased as indicated by the exigencies of the therapeutic or prophylactic situation.
• compositions of the present invention may be carried out using known procedures, at dosages and for periods of time effective to treat a disease or disorder contemplated herein.
• An effective amount of the therapeutic compound necessary to achieve a therapeutic effect may vary according to factors such as the activity of the particular compound employed; the time of administration; the rate of excretion of the compound; the duration of the treatment; other drugs, compounds or materials used in combination with the compound; the state of the disease or disorder, age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well-known in the medical arts. Dosage regimens may be adjusted to provide the optimum therapeutic response.
• an effective dose range for a therapeutic compound of the invention is from about 0.01 mg/kg to 100 mg/kg of body weight/per day.
• One of ordinary skill in the art would be able to study the relevant factors and make the determination regarding the effective amount of the therapeutic compound without undue experimentation.
• the compound may be administered to an animal as frequently as several times daily, or it may be administered less frequently, such as once a day, once a week, once every two weeks, once a month, or even less frequently, such as once every several months or even once a year or less.
• the amount of compound dosed per day may be administered, in non-limiting examples, every day, every other day, every 2 days, every 3 days, every 4 days, or every 5 days.
• a 5 mg per day dose may be initiated on Monday with a first subsequent 5 mg per day dose administered on Wednesday, a second subsequent 5 mg per day dose administered on Friday, and so on.
• the frequency of the dose is readily apparent to the skilled artisan and depends upon a number of factors, such as, but not limited to, type and severity of the disease being treated, and type and age of the animal.
• Actual dosage levels of the active ingredients in the pharmaceutical compositions of this invention may be varied so as to obtain an amount of the active ingredient that is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient.
• a medical doctor e.g., physician or veterinarian, having ordinary skill in the art may readily determine and prescribe the effective amount of the pharmaceutical composition required.
• physician or veterinarian could start doses of the compounds of the invention employed in the pharmaceutical composition at levels lower than that required in order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved.
• Dosage unit form refers to physically discrete units suited as unitary dosages for the patients to be treated; each unit containing a predetermined quantity of therapeutic compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical vehicle.
• the dosage unit forms of the invention are dictated by and directly dependent on (a) the unique characteristics of the therapeutic compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding/formulating such a therapeutic compound for the treatment of a disease or disorder in a patient.
• compositions of the invention are administered to the patient in dosages that range from one to five times per day or more.
• the compositions of the invention are administered to the patient in range of dosages that include, but are not limited to, once every day, every two days, every three days to once a week, and once every two weeks.
• the frequency of administration of the various combination compositions of the invention will vary from subject to subject depending on many factors including, but not limited to, age, disease or disorder to be treated, gender, overall health, and other factors.
• the invention should not be construed to be limited to any particular dosage regime and the precise dosage and composition to be administered to any patient will be determined by the attending physician taking all other factors about the patient into account.
• Compounds of the invention for administration may be in the range of from about 1 pg to about 7,500 mg, about 20 pg to about 7,000 mg, about 40 pg to about 6,500 mg, about 80 p g to about 6,000 mg, about 100 p g to about 5,500 mg, about 200 p g to about 5,000 mg, about 400 p g to about 4,000 mg, about 800 p g to about 3,000 mg, about 1 mg to about 2,500 mg, about 2 mg to about 2,000 mg, about 5 mg to about 1,000 mg, about 10 mg to about 750 mg, about 20 mg to about 600 mg, about 30 mg to about 500 mg, about 40 mg to about 400 mg, about 50 mg to about 300 mg, about 60 mg to about 250 mg, about 70 mg to about 200 mg, about 80 mg to about 150 mg, and any and all whole or partial increments there-in-between.
• the dose of a compound of the invention is from about 0.5 pg and about 5,000 mg. In some embodiments, a dose of a compound of the invention used in compositions described herein is less than about 5,000 mg, or less than about 4,000 mg, or less than about 3,000 mg, or less than about 2,000 mg, or less than about 1,000 mg, or less than about 800 mg, or less than about 600 mg, or less than about 500 mg, or less than about 200 mg, or less than about 50 mg.
• a dose of a second compound as described herein is less than about 1,000 mg, or less than about 800 mg, or less than about 600 mg, or less than about 500 mg, or less than about 400 mg, or less than about 300 mg, or less than about 200 mg, or less than about 100 mg, or less than about 50 mg, or less than about 40 mg, or less than about 30 mg, or less than about 25 mg, or less than about 20 mg, or less than about 15 mg, or less than about 10 mg, or less than about 5 mg, or less than about 2 mg, or less than about 1 mg, or less than about 0.5 mg, and any and all whole or partial increments thereof.
• the present invention is directed to a packaged
• composition comprising a container holding a therapeutically effective amount of a compound of the invention, alone or in combination with a second
• the term“container” includes any receptacle for holding the pharmaceutical composition or for managing stability or water uptake.
• the container is the packaging that contains the pharmaceutical composition, such as liquid (solution and suspension), semisolid, lyophilized solid, solution and powder or lyophilized formulation present in dual chambers.
• the container is not the packaging that contains the pharmaceutical composition, i.e., the container is a receptacle, such as a box or vial that contains the packaged pharmaceutical composition or unpackaged pharmaceutical composition and the instructions for use of the pharmaceutical composition.
• packaging techniques are well known in the art.
• the instructions for use of the pharmaceutical composition may be contained on the packaging containing the pharmaceutical composition, and as such the instructions form an increased functional relationship to the packaged product.
• the instructions may contain information pertaining to the compound’s ability to perform its intended function, e.g., treating, preventing, or reducing a disease or disorder in a patient. Administration
• Routes of administration of any of the compositions of the invention include inhalational, oral, nasal, rectal, parenteral, sublingual, transdermal, transmucosal (e.g, sublingual, lingual, (trans)buccal, (trans)urethral, vaginal (e.g., trans- and perivaginally), (intra)nasal, and (trans)rectal), intravesical, intrapulmonary, intraduodenal, intragastrical, intrathecal, epidural, intrapleural, intraperitoneal, subcutaneous, intramuscular, intradermal, intra-arterial, intravenous, intrabronchial, inhalation, and topical administration.
• inhalational e.g, sublingual, lingual, (trans)buccal, (trans)urethral, vaginal (e.g., trans- and perivaginally), intravesical, intrapulmonary, intraduodenal, intragastrical, intrathecal, epidural, intrapleural, intraperitoneal,
• compositions and dosage forms include, for example, tablets, capsules, caplets, pills, gel caps, troches, emulsions, dispersions, suspensions, solutions, syrups, granules, beads, transdermal patches, gels, powders, pellets, magmas, lozenges, creams, pastes, plasters, lotions, discs, suppositories, liquid sprays for nasal or oral administration, dry powder or aerosolized formulations for inhalation, compositions and formulations for intravesical administration and the like. It should be understood that the formulations and compositions that would be useful in the present invention are not limited to the particular formulations and compositions that are described herein.
• compositions intended for oral use may be prepared according to any method known in the art and such compositions may contain one or more agents selected from the group consisting of inert, non-toxic, generally recognized as safe (GRAS) pharmaceutically excipients which are suitable for the manufacture of tablets.
• GRAS inert, non-toxic, generally recognized as safe
• excipients include, for example an inert diluent such as lactose; granulating and disintegrating agents such as cornstarch; binding agents such as starch; and lubricating agents such as magnesium stearate.
• Tablets may be non-coated or they may be coated using known methods to achieve delayed disintegration in the gastrointestinal tract of a subject, thereby providing sustained release and absorption of the active ingredient.
• a material such as glyceryl monostearate or glyceryl distearate may be used to coat tablets.
• tablets may be coated using methods described in U.S. Patents Nos. 4,256,108; 4,160,452; and 4,265,874 to form osmotically controlled release tablets.
• Tablets may further comprise a sweetening agent, a flavoring agent, a coloring agent, a preservative, or some combination of these in order to provide for pharmaceutically elegant and palatable preparation.
• Hard capsules comprising the active ingredient may be made using a
• the capsules comprise the active ingredient, and may further comprise additional ingredients including, for example, an inert solid diluent such as calcium carbonate, calcium phosphate, or kaolin.
• an inert solid diluent such as calcium carbonate, calcium phosphate, or kaolin.
• Hard capsules comprising the active ingredient may be made using a physiologically degradable composition, such as gelatin. Such hard capsules comprise the active ingredient, and may further comprise additional ingredients including, for example, an inert solid diluent such as calcium carbonate, calcium phosphate, or kaolin.
• an inert solid diluent such as calcium carbonate, calcium phosphate, or kaolin.
• Soft gelatin capsules comprising the active ingredient may be made using a physiologically degradable composition, such as gelatin from animal-derived collagen or from a hypromellose, a modified form of cellulose, and manufactured using optional mixtures of gelatin, water and plasticizers such as sorbitol or glycerol.
• a physiologically degradable composition such as gelatin from animal-derived collagen or from a hypromellose, a modified form of cellulose, and manufactured using optional mixtures of gelatin, water and plasticizers such as sorbitol or glycerol.
• Such soft capsules comprise the active ingredient, which may be mixed with water or an oil medium such as peanut oil, liquid paraffin, or olive oil.
• the compounds of the invention may be in the form of tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents; fillers; lubricants; disintegrates; or wetting agents.
• the tablets may be coated using suitable methods and coating materials such as OPADRY® film coating systems available from Colorcon, West Point, Pa. (e.g ., OPADRY® OY Type, OYC Type, Organic Enteric OY-P Type, Aqueous Enteric OY-A Type, OY-PM Type and
• a tablet comprising the active ingredient may, for example, be made by compressing or molding the active ingredient, optionally with one or more additional ingredients.
• Compressed tablets may be prepared by compressing, in a suitable device, the active ingredient in a free-flowing form such as a powder or granular preparation, optionally mixed with one or more of a binder, a lubricant, an excipient, a surface-active agent, and a dispersing agent. Molded tablets may be made by molding, in a suitable device, a mixture of the active ingredient, a pharmaceutically acceptable carrier, and at least sufficient liquid to moisten the mixture.
• Pharmaceutically acceptable excipients used in the manufacture of tablets include, but are not limited to, inert diluents, granulating and disintegrating agents, binding agents, and lubricating agents.
• Known dispersing agents include, but are not limited to, potato starch and sodium starch glycolate.
• Known surface-active agents include, but are not limited to, sodium lauryl sulphate.
• Known diluents include, but are not limited to, calcium carbonate, sodium carbonate, lactose, microcrystalbne cellulose, calcium phosphate, calcium hydrogen phosphate, and sodium phosphate.
• Known granulating and disintegrating agents include, but are not limited to, com starch and alginic acid.
• Known binding agents include, but are not limited to, gelatin, acacia, pre-gelatinized maize starch, polyvinylpyrrolidone, and hydroxypropyl methylcellulose.
• Known lubricating agents include, but are not limited to, magnesium stearate, stearic acid, silica, and talc.
• Granulating techniques are well known in the pharmaceutical art for modifying starting powders or other particulate materials of an active ingredient.
• the powders are typically mixed with a binder material into larger permanent free-flowing agglomerates or granules referred to as a“granulation.”
• solvent-using“wet” granulation processes are generally characterized in that the powders are combined with a binder material and moistened with water or an organic solvent under conditions resulting in the formation of a wet granulated mass from which the solvent must then be evaporated.
• Melt granulation generally consists in the use of materials that are solid or semi-solid at room temperature (i.e., having a relatively low softening or melting point range) to promote granulation of powdered or other materials, essentially in the absence of added water or other liquid solvents.
• the low melting solids when heated to a temperature in the melting point range, liquefy to act as a binder or granulating medium.
• the liquefied solid spreads itself over the surface of powdered materials with which it is contacted, and on cooling, forms a solid granulated mass in which the initial materials are bound together.
• the resulting melt granulation may then be provided to a tablet press or be encapsulated for preparing the oral dosage form.
• Melt granulation improves the dissolution rate and bioavailability of an active (i.e., drug) by forming a solid dispersion or solid solution.
• U.S. Patent No. 5,169,645 discloses directly compressible wax-containing granules having improved flow properties.
• the granules are obtained when waxes are admixed in the melt with certain flow improving additives, followed by cooling and granulation of the admixture.
• certain flow improving additives such as sodium bicarbonate
• the present invention also includes a multi-layer tablet comprising a layer providing for the delayed release of one or more compounds useful within the methods of the invention, and a further layer providing for the immediate release of one or more compounds useful within the methods of the invention.
• a gastric insoluble composition may be obtained in which the active ingredient is entrapped, ensuring its delayed release.
• Liquid preparation for oral administration may be in the form of solutions, syrups or suspensions.
• the liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, methyl cellulose or hydrogenated edible fats); emulsifying agent (e.g., lecithin or acacia); non- aqueous vehicles (e.g., almond oil, oily esters or ethyl alcohol); and preservatives (e.g., methyl or propyl para-hydroxy benzoates or sorbic acid).
• suspending agents e.g., sorbitol syrup, methyl cellulose or hydrogenated edible fats
• emulsifying agent e.g., lecithin or acacia
• non- aqueous vehicles e.g., almond oil, oily esters or ethyl alcohol
• preservatives e.g., methyl or propyl para-hydroxy benzoates or sorb
• Parenteral administration thus includes, but is not limited to, administration of a pharmaceutical composition by injection of the composition, by application of the composition through a surgical incision, by application of the composition through a tissue-penetrating non-surgical wound, and the like.
• parenteral administration is contemplated to include, but is not limited to, subcutaneous, intravenous, intraperitoneal, intramuscular, intrastemal injection, and kidney dialytic infusion techniques.
• Formulations of a pharmaceutical composition suitable for parenteral administration comprise the active ingredient combined with a pharmaceutically acceptable carrier, such as sterile water or sterile isotonic saline.
• a pharmaceutically acceptable carrier such as sterile water or sterile isotonic saline.
• Such formulations may be prepared, packaged, or sold in a form suitable for bolus administration or for continuous administration.
• Injectable formulations may be prepared, packaged, or sold in unit dosage form, such as in ampules or in multidose containers containing a preservative. Injectable formulations may also be prepared, packaged, or sold in devices such as patient-controlled analgesia (PCA) devices.
• PCA patient-controlled analgesia
• Formulations for parenteral administration include, but are not limited to, suspensions, solutions, emulsions in oily or aqueous vehicles, pastes, and implantable sustained-release or biodegradable formulations. Such formulations may further comprise one or more additional ingredients including, but not limited to, suspending, stabilizing, or dispersing agents.
• the active ingredient is provided in dry (i.e., powder or granular) form for reconstitution with a suitable vehicle (e.g., sterile pyrogen-free water) prior to parenteral administration of the reconstituted composition.
• a suitable vehicle e.g., sterile pyrogen-free water
• the pharmaceutical compositions may be prepared, packaged, or sold in the form of a sterile injectable aqueous or oily suspension or solution.
• This suspension or solution may be formulated according to the known art, and may comprise, in addition to the active ingredient, additional ingredients such as the dispersing agents, wetting agents, or suspending agents described herein.
• additional ingredients such as the dispersing agents, wetting agents, or suspending agents described herein.
• Such sterile injectable formulations may be prepared using a non toxic parenterally acceptable diluent or solvent, such as water or l,3-butanediol, for example.
• Other acceptable diluents and solvents include, but are not limited to, Ringer’s solution, isotonic sodium chloride solution, and fixed oils such as synthetic mono- or di-glycerides.
• compositions for sustained release or implantation may comprise pharmaceutically acceptable polymeric or hydrophobic materials such as an emulsion, an ion exchange resin, a sparingly soluble polymer, or a sparingly soluble salt.
• stratum comeum layer of the epidermis An obstacle for topical administration of pharmaceuticals is the stratum comeum layer of the epidermis.
• the stratum comeum is a highly resistant layer comprised of protein, cholesterol, sphingolipids, free fatty acids and various other lipids, and includes comified and living cells.
• One of the factors that limit the penetration rate (flux) of a compound through the stratum comeum is the amount of the active substance that can be loaded or applied onto the skin surface. The greater the amount of active substance which is applied per unit of area of the skin, the greater the concentration gradient between the skin surface and the lower layers of the skin, and in turn the greater the diffusion force of the active substance through the skin. Therefore, a formulation containing a greater concentration of the active substance is more likely to result in penetration of the active substance through the skin, and more of it, and at a more consistent rate, than a formulation having a lesser concentration, all other things being equal.
• Formulations suitable for topical administration include, but are not limited to, liquid or semi-liquid preparations such as liniments, lotions, oil-in-water or water-in-oil emulsions such as creams, ointments or pastes, and solutions or suspensions.
• Topically administrable formulations may, for example, comprise from about 1% to about 10% (w/w) active ingredient, although the concentration of the active ingredient may be as high as the solubility limit of the active ingredient in the solvent.
• Formulations for topical administration may further comprise one or more of the additional ingredients described herein. Enhancers of permeation may be used. These materials increase the rate of penetration of drugs across the skin.
• Typical enhancers in the art include ethanol, glycerol monolaurate, PGML (polyethylene glycol monolaurate), dimethylsulfoxide, and the like.
• Other enhancers include oleic acid, oleyl alcohol, ethoxy diglycol, laurocapram,
• alkanecarboxylic acids dimethylsulfoxide, polar lipids, or N-methyl-2-pyrrolidone.
• compositions of the invention may contain liposomes.
• the composition of the liposomes and their use are known in the art (i.e., U.S. Patent No. 6,323,219).
• the topically active pharmaceutical composition may be optionally combined with other ingredients such as adjuvants, anti-oxidants, chelating agents, surfactants, foaming agents, wetting agents, emulsifying agents, viscosifiers, buffering agents, preservatives, and the like.
• a permeation or penetration enhancer is included in the composition and is effective in improving the percutaneous penetration of the active ingredient into and through the stratum comeum with respect to a composition lacking the permeation enhancer.
• compositions may further comprise a hydrotropic agent, which functions to increase disorder in the structure of the stratum comeum, and thus allows increased transport across the stratum comeum.
• hydrotropic agents such as isopropyl alcohol, propylene glycol, or sodium xylene sulfonate, are known to those of skill in the art.
• the topically active pharmaceutical composition should be applied in an amount effective to affect desired changes.
• amount effective shall mean an amount sufficient to cover the region of skin surface where a change is desired.
• An active compound should be present in the amount of from about 0.0001% to about 15% by weight volume of the composition. For example, it should be present in an amount from about 0.0005% to about 5% of the composition; for example, it should be present in an amount of from about 0.001% to about 1% of the composition.
• Such compounds may be synthetically-or naturally derived.
• a pharmaceutical composition of the invention may be prepared, packaged, or sold in a formulation suitable for buccal administration.
• a formulation suitable for buccal administration may, for example, be in the form of tablets or lozenges made using conventional methods, and may contain, for example, 0.1 to 20% (w/w) of the active ingredient, the balance comprising an orally dissolvable or degradable composition and, optionally, one or more of the additional ingredients described herein.
• formulations suitable for buccal administration may comprise a powder or an aerosolized or atomized solution or suspension comprising the active ingredient.
• Such powdered, aerosolized, or aerosolized formulations when dispersed, may have an average particle or droplet size in the range from about 0.1 to about 200 nanometers, and may further comprise one or more of the additional ingredients described herein.
• the examples of formulations described herein are not exhaustive and it is understood that the invention includes additional modifications of these and other formulations not described herein, but which are known to those of skill in the art.
• a pharmaceutical composition of the invention may be prepared, packaged, or sold in a formulation suitable for rectal administration.
• a composition may be in the form of, for example, a suppository, a retention enema preparation, and a solution for rectal or colonic irrigation.
• Suppository formulations may be made by combining the active ingredient with a non-irritating pharmaceutically acceptable excipient which is solid at ordinary room temperature (i.e.. about 20°C) and which is liquid at the rectal temperature of the subject (i.e.. about 37°C in a healthy human).
• Suitable pharmaceutically acceptable excipients include, but are not limited to, cocoa buher, polyethylene glycols, and various glycerides.
• Suppository formulations may further comprise various additional ingredients including, but not limited to, antioxidants, and preservatives.
• Retention enema preparations or solutions for rectal or colonic irrigation may be made by combining the active ingredient with a pharmaceutically acceptable liquid carrier.
• enema preparations may be administered using, and may be packaged within, a delivery device adapted to the rectal anatomy of the subject.
• Enema preparations may further comprise various additional ingredients including, but not limited to, antioxidants, and preservatives.
• Additional dosage forms of this invention include dosage forms as described in U.S. Patents Nos. 6,340,475, 6,488,962, 6,451,808, 5,972,389, 5,582,837, and 5,007,790.
• Additional dosage forms of this invention also include dosage forms as described in U.S. Patent Applications Nos. 20030147952, 20030104062, 20030104053, 20030044466, 20030039688, and 20020051820. Additional dosage forms of this invention also include dosage forms as described in PCT Applications Nos. WO 03/35041, WO 03/35040, WO 03/35029, WO 03/35177, WO 03/35039, WO 02/96404, WO 02/32416, WO 01/97783, WO 01/56544, WO 01/32217, WO 98/55107, WO 98/11879, WO 97/47285, WO 93/18755, and WO 90/11757.
• compositions and/or formulations of the present invention may be, but are not limited to, short-term, rapid-offset, as well as controlled, for example, sustained release, delayed release and pulsatile release formulations.
• sustained release is used in its conventional sense to refer to a drug formulation that provides for gradual release of a drug over an extended period of time, and that may, although not necessarily, result in substantially constant blood levels of a drug over an extended time period.
• the period of time may be as long as a month or more and should be a release which is longer that the same amount of agent administered in bolus form.
• the compounds may be formulated with a suitable polymer or hydrophobic material which provides sustained release properties to the compounds.
• the compounds for use the method of the invention may be administered in the form of microparticles, for example, by injection or in the form of wafers or discs by implantation.
• the compounds useful within the invention are administered to a subject, alone or in combination with another pharmaceutical agent, using a sustained release formulation.
• delayed release is used herein in its conventional sense to refer to a drug formulation that provides for an initial release of the drug after some delay following drug administration and that may, although not necessarily, include a delay of from about 10 minutes up to about 12 hours.
• pulsatile release is used herein in its conventional sense to refer to a drug formulation that provides release of the drug in such a way as to produce pulsed plasma profiles of the drug after drug administration.
• immediate release is used in its conventional sense to refer to a drug formulation that provides for release of the drug immediately after drug administration.
• short-term refers to any period of time up to and including about 8 hours, about 7 hours, about 6 hours, about 5 hours, about 4 hours, about 3 hours, about 2 hours, about 1 hour, about 40 minutes, about 20 minutes, or about 10 minutes and any or all whole or partial increments thereof after drug administration after drug administration.
• rapid-offset refers to any period of time up to and including about 8 hours, about 7 hours, about 6 hours, about 5 hours, about 4 hours, about 3 hours, about 2 hours, about 1 hour, about 40 minutes, about 20 minutes, or about 10 minutes, and any and all whole or partial increments thereof after drug administration.
• reaction conditions including but not limited to reaction times, reaction size/volume, and experimental reagents, such as solvents, catalysts, pressures, atmospheric conditions, e.g., nitrogen atmosphere, and reducing/oxidizing agents, are within the scope of the present application.
• range such as from 1 to 6 should be considered to have specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 2.7, 3, 4, 5, 5.3, and 6. This applies regardless of the breadth of the range.
• HepDEl9 cell culture system is a HepG2 (human hepatocarcinoma) derived cell line that supports HBV DNA replication and cccDNA formation in a tetracycline (Tet)-regulated manner and produces HBV rcDNA and a detectable reporter molecule dependent on the production and maintenance of cccDNA (Guo, et al., 2007, J. Virol. 81: 12472-12484).
• HepDEl9 (50,000 cells/well) were plated in 96-well collagen-coated tissue-culture treated microtiter plates in DMEM/F12 medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin and 1 pg/mL tetracycline and incubated in a humidified incubator at 37 °C and 5% C0 2 overnight. Next day, the cells were switched to fresh medium without tetracycline and incubated for 4 hours at 37 °C and 5% C0 2 . The cells were treated with fresh Tet-free medium with compounds at concentrations starting at 25 mM and a serial, 1 ⁇ 2 log, 8- point, titration series in duplicate.
• the final DMSO concentration in the assay was 0.5%.
• the plates were incubated for 7 days in a humidified incubator at 37 °C and 5% C0 2 . Following a 7 day-incubation, the level of rcDNA present in the inhibitor-treated wells was measured using a Quantigene 2.0 bDNA assay kit (Affymetrix, Santa Clara, CA) with HBV specific custom probe set and manufacturers instructions. Concurrently, the effect of compounds on cell viability was assessed using replicate plates, plated at a density of 5,000 cells/well and incubated for 4 days, to determine the ATP content as a measure of cell viability using the cell-titer glo reagent (CTG; Promega Corporation, Madison, WI) as per manufacturer’s instructions.
• CCG Cell-titer glo reagent
• the plates were read using a Victor luminescence plate reader (PerkinElmer Model 1420 Multilabel counter) and the relative luminescence units (RLU) data generated from each well was calculated as % inhibition of the untreated control wells and analyzed using XL-Fit module in Microsoft Excel to determine EC 50 and EC90 (bDNA) and CC50 (CTG) values using a 4-parameter curve fitting algorithm.
• RLU relative luminescence units
• HPLC Method B Waters 2695/2998 system employing a Xbridge C18, 5m, 150 x 4.6 mm column with an aqueous component of 0.05% trifluoroacetic acid in water and an organic component of 0.05% trifluoroacetic acid in acetonitrile.
• Enantiomer I or“Diastereomer I” refers to the first enantiomer or diastereomer eluded from the chiral column under the specific chiral analytical conditions detailed for examples provided elsewhere herein; and“Enantiomer II” or“Diastereomer II” refers to the second enantiomer or diastereomer eluded from the chiral column under the specific chiral analytical conditions detailed for examples provided elsewhere herein.
• Such nomenclature does not imply or impart any particular relative and/or absolute configuration for these compounds.
• reaction mixture was stirred at room temperature for 16 h and then quenched by the addition of 100 mL of sat. ammonium chloride solution.
• the mixture was extracted with 3 x 100 mL of ethyl acetate and the combined organic extracts were washed with 100 mL of water, 100 mL of brine, dried (Na ⁇ SCL) and the solvent was removed in vacuo.
• the residue was purified by flash chromatography (SiCf. eluting with a linear gradient of 30-50% ethyl acetate/petroleum ether) to provide 2.5 g (20.6 mmol, 34%) of 5.6-dihydrocyclopenta
• the mixture was then stirred at 100 °C under 200 psi of carbon monoxide gas for 16 h.
• the mixture was allowed to cool to room temperature, filtered through CELITE® and the pad was washed with 20 mL of ethanol.
• the solvent was removed in vacuo and the residue was resuspended in 100 mL of water.
• the mixture was extracted with 3 x 80 mL of ethyl acetate and the combined organic extracts were washed with 50 mL of water, 50 mL of brine, dried (Na ⁇ SCL). filtered and the solvent was removed in vacuo.
• the residue was purified by flash chromatography (SiCL.
• cyclopenta[c]pyrrole-l-carboxylic acid (Vila) in 35 mL of DMF at 0 °C was added 9.7 g (75.4 mmol, 3.0 eq.) of /V,/V-diisopropylethylamine followed by 14.3 g (37.6 mmol, 1.5 eq.) of hexafluorophosphate azabenzotriazole tetramethyl uronium (HATU) and 5.4 g (37.2 mmol, 1.5 eq.) of 3-chloro-4-fluoro aniline, and the mixture was heated at 60 °C for 16 h.
• HATU hexafluorophosphate azabenzotriazole tetramethyl uronium
• the reaction mixture was then stirred at 65 °C for 16 h.
• the mixture was allowed to cool to room temperature and diluted with 100 mL of water.
• the mixture was extracted with 3 x 100 mL of ethyl acetate and the combined organic extracts were washed with 80 mL of water, 80 mL of brine, dried (Na ⁇ SCL). filtered and the solvent was removed in vacuo.
• the residue was purified by flash chromatography (SiCL. eluted with a linear gradient of 0-4% methanol in methylene chloride) to provide 0.55 g (1.23 mmol,
• Oxazol-2-ylmethyl (l-((3-chloro-4-fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydro cyclopenta[c]pyrrol-4-yl)carbamate was synthesized in a similar manner as described above from 4-amino-iV-(3-chloro-4-riuorophenyl)-2-methyl-2.4.5.6- tetrahydrocyclopenta
• Oxazol-5-ylmethyl (l-((3-chloro-4-fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydro cyclopenta[c]pyrrol-4-yl)carbamate was synthesized in a similar manner as described above from 4-amino-/V-(3-chloro-4-fluorophenyl)-2-methyl-2, 4,5, 6-tetrahydrocy clopenta[c]pyrrole- 1 -carboxamide (IXa) and oxazol-5-ylmethyl 1 //-imidazole- 1 -carboxylate (Xlllf).
• But-2-yn-l-yl (1 -((3-chloro-4-fluoropheny l)carbamoyl)-2-methyl-2, 4,5,6- tetrahydrocyclopenta[c]pyrrol-4-yl)carbamate was synthesized in a similar manner as described above from 4-amino-/V-(3-chloro-4-fluorophenyl)-2-methyl-2, 4,5,6- tetrahydrocyclopenta [c] pyrrole- 1 -carboxamide (IXa) and but-2-yn-l-yl 1 //-imidazole- 1 - carboxylate (XHIi).
• Pent-2-yn-l-yl (l-((3-chloro-4-fluorophenyl)carbamoyl)-2-methyl-2, 4,5,6- tetrahydrocyclopenta[c]pyrrol-4-yl)carbamate was synthesized in a similar manner as described above from 4-amino-/V-(3-chloro-4-fluorophenyl)-2-methyl-2, 4,5,6- tetrahydrocyclopenta [c] pyrrole- 1 -carboxamide (IXa) and pent-2-yn-l-yl 1 //-imidazole- 1 - carboxylate (XHIj).
• Racemic isopropyl l-((((l-((3-chloro-4-fluorophenyl)carbamoyl)-2-methyl-2, 4,5,6- tetrahydrocyclopenta [c]pyrrol-4-yl)carbamoyl)oxy)methyl)-3,3-difluorocyclobutane-l- carboxylate (46) was synthesized in a similar manner as described above from 4-amino-/V-(3- chloro-4-fluorophenyl)-2-methyl-2,4,5,6- tetrahydrocyclopenta [c]pyrrole-l -carboxamide (IXa) and (3,3-difluoro-l-(isopropoxycarbonyl)cyclobutyl)methyl 1 //-imidazole- 1 - carboxylate (XHIm).
• EXAMPLE 21 (l-(Tetrahydro-2//-pyran-2-yl)-l//-pyrazol-3-yl)methyl (l-((3-chloro-4- fluorophenyl) carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (63) l-(Tetrahydro-2//-pyran-2-yl)-l//-pyrazole-3-carbaldehyde:
• the resulting solution was extracted with 3 x 40 mL of ethyl acetate and the combined organic extracts were washed with 40 mL of water, 40 mL of brine, dried (Na 2 S04), filtered and the solvent was removed in vacuo.
• the residue comprising a mixture of regioisomers was purified by MPLC (REVELERIS® silica column, eluting with a linear gradient of 4-10% ethyl acetate in petroleum ether) to provide 350 mg of ethyl 2-methyl-2//- 1 2.3-triazole-4- carboxylate (A) along with 540 mg of an isomeric mixture (41% & 55% of A and B
• VUIb A-(4-Fluoro-3-methylphenyl)-2-methyl-4-oxo-2, 4,5,6- tetrahydrocyclopenta[c]pyrrole-l- carboxamide
• HATU hexafluorophosphate azabenzotriazole tetramethyl uronium
• HATU hexafluorophosphate azabenzotriazole tetramethyl uronium
• 3 x 50 mL of ethyl acetate The combined organic extracts were washed with 50 mL of brine, dried (Na ⁇ SCL) and the solvent was removed in vacuo. The residue was purified by flash chromatography (SiCL.
• VUIb /V-(4-fluoro-3-methylphenyl)-2- methyl-4-oxo-2,4,5,6-tetrahydrocyclopenta[c]pyrrole-l-carboxamide
• the mixture was then cooled to 0 °C and diluted with 50 mL of ice-cold water.
• the heterogeneous mixture was filtered through CELITE® and the pad was washed with 40 mL of ethyl acetate.
• the organic phase was separated from the filtrate and the aqueous layer was extracted with 3 x 30 mL of ethyl acetate.
• the combined organic extracts were washed with 80 mL of brine, dried (Na ⁇ SCL). filtered and the solvent was removed in vacuo.
• reaction was quenched with 30 mL of ice-cold water and extracted with 3 x 50 mL of ethyl acetate. The combined organic extracts were washed with 40 mL of brine, dried (Na ⁇ SCL). filtered the solvent was removed in vacuo.
• EXAMPLE 36 (5-Methyl-2//-l,2,3-triazol-4-yl)methyl (l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (138, 139)
• a sealed tube containing 1.6 g (14.28 mmol, 1.0 eq.) of ethyl but-2-ynoate and 5.8 g (35.74 mmol, 7.0 eq.) of azidotrimethylsilane in 16 mL of THF was heated to 100 °C for 30 h. The mixture was allowed to cool to room temperature and the solvent was removed in vacuo. The residue suspended in 40 mL of water and extracted with 3 x 40 mL of ethyl acetate. The combined organic extracts were washed with 30 mL of water, 40 mL of brine, dried (Na ⁇ SCL) and the solvent was removed in vacuo.
• reaction was quenched by the addition of 40 mL of ice-cold water and extracted with 3 x 75 mL of ethyl acetate. The combined organic extracts were washed with 80 mL of brine, dried (Na ⁇ SCL). filtered and the solvent was removed in vacuo. The residue was purified by MPLC (REVELERIS® Silica column, eluting with a linear gradient of 10-40% ethyl acetate/petroleum ether) to provide 0.4 g (1.13 mmol, 41%) of (5-methyl-2-trityl-2//- 1 2.3-triazol-4-yl)methanol (Xllr).
• EXAMPLE 37 (5-Ethyl-2//-l,2,3-triazol-4-yl)methyl (l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (140, 141)
• EXAMPLE 38 (5-Cyclopropyl-2//-l,2,3-triazol-4-yl)methyl (l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (146, 147)
• Racemic (2//-tetra/ol-5-yl (methyl (1 -((3-chloro-4-fluorophenyl)carbamoyl)-2-methyl- 2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4-yl)carbamate (148) was synthesized in a similar manner as described above from 4-amino-/V-(3-chloro-4-fluorophenyl)-2 -methyl-2, 4,5,6- tetrahydrocyclopenta[c]pyrrole-l -carboxamide (IXa) and (2-trityl-2//-tetrazol-5-yl (methyl 1 //-imidazole- 1 -carboxylate (XIIIu) followed by acid mediated detritylation. The enantiomers were subsequently separated by SFC. Method: isocratic, Mobile phase MeOH: C0 2 - 15:85. Column: Chiralpak IG (30 x 250 mm),
• EXAMPLE 40 ( 1-Methyl- li7-tetrazol-5-yl)methyl (l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (144, 145)
• the mixture was diluted with 70 mL of water and extracted with 3 x 50 mL of ethyl acetate.
• the combined organic extracts were washed with 50 mL of water, 50 mL of brine, dried (Na ⁇ SCf). filtered and the solvent was removed in vacuo.
• Ester hydrolysis of XIV to generate XV followed by carboxylic acid functionalization provides XVI (or alternatively, XIV can be converted directly to XVI through direct animation of the ester) followed by reductive amination to provide XVIII allows for /V-functionalization to provide XIX.
• EXAMPLE 44 ( 1 - Methyl- 1 H- 1 ,2,4-triazol-3-yl)methyl (3-chloro-l-((3-chloro-4- iluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyiTol-4- yl)carbamate (51, 52)
• the mixture was cooled to 0 °C and 0.27 g (7.10 mmol, 4.0 eq.) of sodium borohydride was added. The mixture was then stirred at 0 °C for 4 h. The mixture was diluted with 100 mL of ice-cold water and extracted with 3 x 100 mL of ethyl acetate. The combined organic extracts were washed with 100 mL of brine, dried (Na ⁇ SCL). filtered and the solvent was removed in vacuo.
• EXAMPLE 46 (2//-l,2,3-Triazol-4-yl)methyl (3-chloro-l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (106, 107)
• the mixture was then allowed to cool to room temperature, diluted with 15 mL of water and the resulting solids collected by filtration and dried under high vacuum. The solids were then triturated with 80 mL of 10% methanol in methylene chloride and filtered.
• EXAMPLE 48 (l//-l,2,4-Triazol-3-yl)methyl (3-bromo-l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (124, 125)
• EXAMPLE 49 ( 1 - Methyl- ⁇ H- ⁇ ,2,4-triazol-3-y l)methyl (l-((3-chloro-4-fluorophenyl) carbamoyl)-2,3-dimethyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4-yl)carbamate (61, 82, 83)
• EXAMPLE 50 ( 1 - Methyl- 1 H- 1 ,2,4-triazol-3-y l)methy 1 (l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-3-phenyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol-4- yl)carbamate (108, 109)
• the mixture was purged with argon for 5 min and 0.46 g (1.42 mmol, 3.0 eq.) of cesium carbonate was added followed by 0.5 mL of water.
• the mixture was purged with argon for an additional 5 min and 16 mg (0.023 mmol, 5 mol%) of palladium acetate was added followed by 16 mg (0.047 mmol, 10 mol%) of Cataxium-A.
• the mixture was then heated to 80 °C for 3 h. On cooling to room temperature, the mixture was filtered through CELITE®, washed with 20 mL of ethyl acetate and the filtrate was concentrated in vacuo.
• EXAMPLE 52 ( 1 - Methyl- 1 H- 1 ,2,4-triazol-3-y l)methy 1 (l-((3-chloro-4- fluorophenyl)carbamoyl)-3-cyclopropyl-2-methyl-2, 4,5,6- tetrahydrocyclopenta[c]pyrrol-4-yl)carbamate (90, 91)
• reaction was quenched with 50 mL of ice-cold water and extracted with 3 x 100 mL of ethyl acetate. The combined organic extracts were washed with 100 mL of brine, dried (Na ⁇ SCL). filtered and the solvent was removed in vacuo. The residue was triturated with diethyl ether to provide 0.75 g (2.07 mmol, 56%) of /V-(3-chloro-4- fluorophenyl)-3-cyclopropyl-4-(hydroxyimino)-2-methyl-2, 4,5,6- tetrahydrocyclopenta[c]pyrrole-l-carboxamide.
• EXAMPLE 54 ( 1 - Methyl- 1 H- 1 ,2,4-triazol-3-y l)methy 1 (l-((3-chloro-4- fluorophenyl)carbamoyl)-3-cyclobutyl-2-methyl-2,4,5,6-tetrahydrocyclopenta[c]pyrrol- 4-yl)carbamate (96, 97)
• EXAMPLE 55 1-Methyl- LFM, 2, 4-triazol-3-yl)methyl (l-((3-chloro-4- fluorophenyl)carbamoyl)-2-methyl-3-(trifluoromethyl)-2, 4,5,6- tetrahydrocyclopenta[c]pyrrol-4-yl)carbamate (122, 123)
• the mixture was then allowed to cool to room temperature and quenched with 50 mL ice-cold water and diluted with 100 mL of ethyl acetate. The resulting solution was filtered, and the filtrate was washed with 50 mL of water. The aqueous phase was extracted with 2 x 50 mL of ethyl acetate and the combined organic extracts were dried (Na 2 S04), filtered and the solvent was removed in vacuo.

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