WO2020021874A1 - Méthode de contrôle de pression positive d'incubateur et système de culture l'utilisant - Google Patents

Méthode de contrôle de pression positive d'incubateur et système de culture l'utilisant Download PDF

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Publication number
WO2020021874A1
WO2020021874A1 PCT/JP2019/022448 JP2019022448W WO2020021874A1 WO 2020021874 A1 WO2020021874 A1 WO 2020021874A1 JP 2019022448 W JP2019022448 W JP 2019022448W WO 2020021874 A1 WO2020021874 A1 WO 2020021874A1
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air
culture
culture chamber
incubator
air supply
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PCT/JP2019/022448
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English (en)
Japanese (ja)
Inventor
川崎　康司
純 益留
和彦 北洞
兼 永井
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株式会社エアレックス
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the present invention relates to an incubator, and more particularly to an incubator capable of stably maintaining a sterile state and a positive pressure state inside a culture chamber while appropriately controlling temperature and humidity suitable for culture in a mobile incubator. And a positive pressure control method. Further, the present invention relates to a culture operation system using a positive pressure control method of an incubator.
  • a hot water heater or an electric heater or the like is generally built in a wall of an incubator, a door, a shelf, or the like, and the room temperature is adjusted by radiant heat from the wall.
  • a humidifying dish is generally provided inside the incubator to store water, and the indoor humidity is adjusted by natural evaporation of the stored water.
  • a carbon dioxide concentration sensor or a nitrogen gas concentration sensor, and a supply path from a carbon dioxide gas cylinder or a nitrogen gas cylinder are generally provided. Adjustments have been made.
  • air may be agitated by an indoor fan to achieve uniformity.
  • the conventional incubator could not guarantee Grade A (Ministry of Health, Labor and Welfare, aseptic drug production guidelines) conforming to GMP (Good Manufacturing Practice). The reason is that even if the interior is sterilized to grade A, the air pressure could not be maintained higher than the external environment to maintain it. Furthermore, water is supplied from the outside along with the evaporation of the stored water inside the incubator, but in this case, there is a problem that the water supplied from the outside adversely affects the internal sterile environment.
  • Grade A Ministry of Health, Labor and Welfare, aseptic drug production guidelines
  • GMP Good Manufacturing Practice
  • Patent Document 2 it has been proposed to provide a filter on the water supply path from outside the incubator to the humidifying dish.
  • the present inventors in Patent Document 3 below, supply steam that has been sterilized at a high temperature from the outside, and can control the air supply device and the exhaust device to maintain the air pressure inside the culture chamber higher than the external environment. Proposed incubator.
  • the incubator of Patent Document 3 is excellent in that sterilization of steam supplied from the outside can be ensured, and that the air pressure in the culture chamber can be higher than the external environment.
  • an air supply device and an exhaust device are controlled by a microcomputer linked to a pressure sensor.
  • a cell culture system in the field of regenerative medicine is composed of an isolator for performing operations such as pretreatment of a culture, and a plurality of incubators connected to the isolator to accommodate the processed culture.
  • the incubator containing the culture is separated from the isolator and moved to the culture station, where the culture is performed for a predetermined time.
  • such a mobile incubator has facilities that can be operated with a minimum number of rechargeable batteries or the like without connecting a distribution line or the like. Therefore, in order to maintain the air pressure inside the culture chamber higher than the external environment, control using a simpler mechanism is desired instead of a large-scale combination of an air supply device and an exhaust device.
  • the present invention can address the above-mentioned problems, maintain the temperature and humidity inside the culture chamber uniformly, and even when the inside of the culture chamber is sterilized to grade A, the sterile environment can be improved. It is an object of the present invention to provide a positive pressure control method for an incubator that can maintain the internal air pressure higher than the external environment by a simple mechanism at all times, and a culture operation system using the method.
  • the present inventors have conducted intensive studies and as a result, by combining the opening area of the air supply port and the exhaust port and the air supply fan, the air supply fan of the culture chamber with respect to the static pressure fluctuation inside the culture chamber.
  • the present invention has been completed by utilizing the fact that the air volume fluctuates.
  • the air supply port includes an air supply unit (30) that supplies air of an external environment to the inside of the culture chamber.
  • the air supply unit includes an air supply fan (32) and a storage battery that drives the air supply fan (32).
  • the air pressure inside the culture chamber is maintained at a predetermined static pressure higher than the external environment by driving the air supply fan,
  • the air pressure in the culture chamber rises due to humidification into the culture chamber by the humidifying means, the air volume decreases along the air volume-static pressure curve of the air supply fan, and the positive pressure state inside the culture chamber increases. Is maintained at the predetermined static pressure, When the air pressure in the culture chamber falls, the air volume increases along the air volume-static pressure curve of the air supply fan, and the positive pressure state inside the culture chamber is maintained at the predetermined static pressure.
  • a positive pressure control method for an incubator according to the first aspect, is not only caused by humidification of the culture chamber by the humidifying means, but also by adjustment corresponding to the variation of the concentration of carbon dioxide or nitrogen in the culture chamber.
  • the humidifying unit includes a compressed gas generating unit that generates a compressed gas, a water supply unit that supplies water, a mixed gas-liquid regulator that adjusts a mixed gas-liquid mixture of the compressed air and the water, A vaporizer (51) for vaporizing gas-liquid to generate water vapor, The steam generated by the vaporizer is directly supplied to the air circulated by the circulation means without passing through an air filter.
  • the culture operation system provides An isolator (10) capable of maintaining a sterile state, and a plurality of incubators (20, 20a to 20h) connectable to the isolator and capable of maintaining a sterile state and transportable; Performing operations necessary for culture inside the isolator to prepare a culture, storing the culture in the incubator connected to the isolator, separating the incubator from the isolator, separating the incubator Is transported to a culture station (110) at a position distant from the isolator, and a plurality of incubators are stored in the culture station to culture the culture.
  • the plurality of incubators are controlled by the positive pressure control method for an incubator according to any one of claims 1 to 3, at a stage of moving to the culture station and / or at a stage of culture.
  • the incubator used in the method for controlling the positive pressure of the incubator according to the present invention has the culture chamber, the temperature control means, and the humidification means.
  • the culture room has an inlet and an outlet.
  • the temperature control means controls the temperature of the air inside the culture room.
  • the humidifying means humidifies the air inside the culture chamber.
  • the air supply port is provided with an air supply means for supplying the air of the external environment to the inside of the culture chamber.
  • the air supply means includes an air supply fan and a storage battery that drives the air supply fan.
  • the air supply fan drives the air pressure inside the culture chamber higher than the external environment (positive pressure state) at a predetermined static pressure. Can be maintained.
  • the air pressure in the culture chamber may fluctuate while the air pressure in the culture chamber is maintained at a predetermined static pressure.
  • the air pressure in the culture chamber rises due to humidification into the culture chamber by the humidifying means.
  • the air volume decreases along the air volume-static pressure curve of the air supply fan, and the amount of air supplied to the inside of the culture chamber decreases.
  • the air pressure in the culture chamber decreases, and the positive pressure state is maintained at a predetermined static pressure.
  • the air pressure in the culture chamber drops due to another factor.
  • the air flow increases along the air flow-static pressure curve of the air supply fan, and the amount of air supplied to the inside of the culture chamber increases.
  • the air pressure in the culture chamber increases, and the positive pressure state is maintained at a predetermined static pressure.
  • the temperature and humidity inside the culture room can be maintained uniform, and even when the inside of the culture room is sterilized to grade A, the sterile environment is easily maintained.
  • the positive pressure control method of the incubator which can maintain the internal air pressure always higher than the external environment by a simple mechanism can be provided.
  • the fluctuation of the air pressure in the culture chamber may be caused not only by humidification of the culture chamber by the humidifying means, but also by the fluctuation of the carbon dioxide concentration or the nitrogen gas concentration in the culture chamber.
  • it can be dealt with by changing the air volume along the air volume-static pressure curve of the air supply fan as described above.
  • the humidifying unit includes the compressed gas generating unit, the water supplying unit, the gas-liquid regulator, and the vaporizer.
  • the mixed gas-liquid regulator mixes the compressed gas generated by the compressed gas generating means with the water supplied by the water supply means to generate a mixed gas-liquid.
  • the vaporizer vaporizes the generated mixed gas-liquid to generate steam. In this way, the water vapor generated in the vaporizer is directly supplied to the air circulated by the circulation means without passing through the air filter.
  • the temperature and humidity inside the culture room can be kept uniform, and dew condensation does not occur inside the culture room. Further, the vapor generated by the vaporizer does not adversely affect the internal sterile environment.
  • the steam supplied by the humidifying means into the culture chamber is a mixed gas of the compressed gas and the vaporized water, and fluctuates the static pressure inside the culture chamber. Even in such a case, according to the above configuration, the positive pressure state in the culture chamber can be maintained at a predetermined static pressure.
  • the culture operation system includes the isolator and the plurality of incubators.
  • Isolators can maintain sterility.
  • the plurality of incubators can be connected to the isolator and maintain a sterile state, and can be separated from the isolator and transported to the culture station. Note that these incubators are controlled by the positive pressure control method for the incubator according to the above configuration in both the moving stage to the culturing station and the culturing stage, or in the moving stage or the culturing stage.
  • This culture operation system is operated as follows. First, an operation necessary for culture is performed inside the isolator to prepare a culture object. Next, the culture is accommodated in an incubator connected to the isolator. Next, the incubator is separated from the isolator. Next, the separated incubator is transported to a culture station at a position away from the isolator. Next, a plurality of incubators are stored in the culture station to culture the culture.
  • the temperature and humidity inside the culture room can be maintained uniform, and even when the inside of the culture room is sterilized to grade A, the sterile environment is easily maintained.
  • a simple mechanism it is possible to provide a culture operation system using a positive pressure control method of an incubator capable of always maintaining the internal air pressure higher than the external environment.
  • FIG. 2 is a schematic side view illustrating a configuration of an incubator used for the culture operation system in FIG. 1.
  • 3 is an air volume-static pressure curve of a fan motor used in the incubator of FIG. 2; It is sectional drawing which shows one Embodiment of the vaporizer with which the humidifier of FIG. 2 is provided.
  • FIG. 1 is a plan view showing the overall configuration of an embodiment of the culture operation system according to the present invention.
  • an isolator 10 is installed inside a clean room 100 (left side in the figure), and an incubator 20 is connected to the isolator 10.
  • the interior of the clean room 100, the interior of the isolator 10 (inside of the chamber), and the interior of the incubator 20 (in the culture chamber) are aseptically sterilized by a sterilization operation in advance.
  • the inside of the chamber of the isolator 10 and the culture room of the incubator 20 are sterilized to grade A according to GMP.
  • a culturing station 110 for storing a plurality of incubators is provided at a position (right side in the figure) away from the isolator 10 inside the clean room 100.
  • a plurality of incubators 20a to 20h accommodating a culture object are stored.
  • the incubator 20 connected to the isolator 10 is separated from the isolator 10 and transported to the culturing station for storage when the work of accommodating the culture is completed (indicated by a dashed arrow in the drawing).
  • a worker (not shown) inside the clean room 100 performs an operation necessary for culture from outside the isolator 10 through a work glove, and transfers a culture solution for culturing cells to a petri dish or the like. Is filling.
  • the petri dish filled with the culture solution is accommodated in the incubator 20 from the inside of the isolator 10.
  • the incubator 20 containing the Petri dish filled with the culture solution is separated from the isolator 10 and transported to the culture station 110 by a worker (not shown) inside the clean room 100. During this transfer, the positive pressure state inside the incubator 20 is maintained.
  • the plurality of incubators 20a to 20h transported to the culture station 110 are stored in the culture station 110, and the cells in the petri dish housed therein are cultured. During this culture, the positive pressure state inside the incubator 20 is maintained.
  • necessary equipment such as a distribution line and a supply / exhaust pipe may be attached to the incubator 20 after being transferred from the isolator 10 to the culture station 110.
  • the configuration of the incubator used in the present invention will be described.
  • the inside of the chamber of the isolator 10 and the culture room of the incubator 20 are sterilized to grade A according to GMP. Therefore, in order to maintain the grade A state, it is preferable that the air pressure inside the isolator 10 and the incubator 20 be maintained at a higher air pressure (positive pressure state) than the air pressure of the clean room 100 (this is the external environment).
  • ⁇ Maintaining the inside of the chamber of the isolator 10 in a positive pressure state is performed by a usual method. That is, the control can be easily performed by controlling the air supply device and the exhaust device by the microcomputer linked with the pressure sensor.
  • a positive pressure state can be maintained by providing the same equipment as the isolator 10 in the culture chamber of the incubator 20. However, in a mobile incubator, it is preferable to use equipment that can operate with a minimum number of rechargeable batteries without connecting distribution lines or the like.
  • FIG. 2 is a schematic side view showing a configuration of an incubator used for the culture operation system.
  • an incubator 20 includes a mount 21 with casters 21 a mounted on a floor, a culture room 22 mounted on the mount 21, and a temperature of air inside the culture room 22.
  • a temperature controller (not shown) for adjusting the temperature; a humidifier 50 for humidifying the air inside the culture chamber 22; a carbon dioxide concentration controller 60 for adjusting the carbon dioxide concentration inside the culture chamber 22;
  • a circulating device (not shown) for circulating the air inside the culture chamber 22.
  • these devices are provided with various sensors for detecting the pressure, temperature, humidity, carbon dioxide gas concentration, nitrogen gas concentration, and the like inside the culture chamber 22 (all are not shown).
  • the carbon dioxide gas concentration control device 60, the nitrogen gas concentration control device 70, the circulation device, and the like may be installed as necessary.
  • connection door 23 is provided on a front wall portion 22a (left side in the figure) of the culture chamber 22 for airtight connection with the isolator 10 and communication with the inside.
  • the structure of the connection door 23 is not particularly limited as long as the connection door 23 can be air-tightly connected to the isolator 10.
  • an air supply port 24 for supplying air to the inside of the culture chamber 22 is opened in the upper wall portion 22b of the culture chamber 22, and an air supply device 30 is connected to the air supply port 24.
  • An air supply duct 30 is connected to the air supply device 30, and a fan motor 32 and a HEPA filter 33 are provided in the duct.
  • an exhaust port 25 for exhausting the air inside the culture chamber 22 is opened in the lower wall portion 22c of the culture chamber 22, and an exhaust duct 41 is connected to the exhaust port 25.
  • the opening area of the air supply port 24 is designed to be larger than the opening area of the exhaust port 25 (the reason will be described later).
  • the fan motor 32 of the air supply device 30 supplies the air inside the clean room 100 to the inside of the culture room 22 of the incubator 20. At this time, the supplied air is further purified by the HEPA filter 33.
  • the capacity of the fan motor 32 is selected to have a predetermined maximum air volume and a maximum static pressure in order to maintain a positive pressure state inside the culture chamber 22 of the incubator 20.
  • the type of the fan motor 32 is not particularly limited, and may be any type such as a propeller fan of an axial fan, a sirocco fan of a centrifugal fan, and a turbo fan.
  • the incubator 20 does not use a distribution line to drive the fan motor 32 in both the movement stage and the culture stage from the isolator 10 to the culture station 110, or in the movement stage or the culture stage.
  • the storage battery (not shown) is provided.
  • an electromagnetic valve or a catalyst layer may be provided in each of the supply duct 31 and the exhaust duct 41.
  • the catalyst layer is used to decompose hydrogen peroxide gas and the like released from the inside of the culture chamber 22 by aeration after decontaminating the inside of the culture chamber 22 of the incubator 20 with hydrogen peroxide gas or the like.
  • FIG. 3 is an air volume-static pressure curve of the fan motor 32 used in the incubator 22.
  • point 4 on the vertical axis represents the maximum static pressure P4 of the fan motor 32, and the air volume is zero at this time. In this state, the air supply does not occur even if the fan motor 32 rotates due to the positive pressure inside the culture chamber 22.
  • point 5 on the horizontal axis represents the maximum air volume L5 of the fan motor 32, and at this time, the static pressure is zero. In this state, the air pressure inside the culture room 22 is the same as the air pressure inside the clean room 100, and the inside of the culture room 22 does not become a positive pressure even if the fan motor 32 rotates.
  • the degree of the positive pressure maintained in the culture chamber 22 is not particularly limited. For example, +30 Pa may be maintained.
  • the positive pressure state can be maintained only by driving the fan motor 32. Therefore, in the present embodiment, the internal structure of the culture chamber 22 and the number of contents (mounting density), the opening area of the air supply port 24 and the opening area of the exhaust port 25 of the culture chamber 22, and the target positive pressure state Can be determined, the model and performance of the fan motor 32, or the set output can be determined.
  • point 1 is that the inside of the culture chamber 22 is maintained in a positive pressure state
  • the static pressure P1 is the target positive pressure (for example, +30 Pa)
  • the air volume L1 is from the air supply port 24 to the culture chamber.
  • the same amount of L1 is supplied from the exhaust port 25 to the inside of the exhaust port 25.
  • the positive pressure state is stably maintained.
  • the cells are cultured inside the culture chamber 22, and a change in humidity, a change in carbon dioxide concentration, a change in nitrogen gas concentration, and the like occur.
  • the humidifier 50 operates to stably maintain the humidity condition (culture condition) inside the culture room 22. It is important to stably maintain the temperature condition and the humidity condition among the culture conditions in the culture room 22. For example, the temperature condition is maintained at 37 ⁇ 0.5 ° C., and the humidity condition needs to maintain a high humidity state of relative humidity of 95 to 100% RH. Therefore, water vapor is supplied from the humidifier 50 to the inside of the culture chamber 22 under the control of a microcomputer linked to the humidity sensor.
  • the supply of steam is not particularly limited, and any method may be used as long as a sterile state can be maintained.
  • a humidifying dish may be provided inside the incubator to store water, and the indoor humidity may be adjusted by natural evaporation of the stored water.
  • a humidifying device 50 that can ensure the sterilization of steam supplied from the outside is employed. The humidifier 50 vaporizes a mixed gas-liquid mixture of compressed air and water to generate and supply steam (details will be described later).
  • the amount of air supplied to the inside of the culture room 22 decreases, and the air pressure inside the culture room 22 decreases.
  • the air pressure inside the culture chamber 22 converges to the set static pressure P1.
  • the increase in the air pressure inside the culture chamber 22 is not caused only by the operation of the humidifier 50, but by the supply of carbon dioxide by the carbon dioxide concentration controller 60 or the supply of nitrogen gas by the nitrogen gas concentration controller 70. Also occurs.
  • the air pressure inside the culture chamber 22 may drop.
  • the airflow of the fan motor 32 increases from L1 to L3.
  • the amount of air supplied to the inside of the culture room 22 increases, and the air pressure inside the culture room 22 increases.
  • the air pressure inside the culture chamber 22 converges to the set static pressure P1.
  • the temperature and humidity inside the culture room can be maintained uniformly, and this sterile environment is maintained even when the inside of the culture room is sterilized to grade A. Therefore, it is possible to provide a positive pressure control method of an incubator that can always maintain the internal air pressure higher than the external environment by a simple mechanism, and a culture operation system using this method.
  • the humidifying device 50 employs a specific humidifying mechanism capable of supplying a trace amount of water vapor to the inside of the culture chamber 22.
  • the humidifier 50 includes a compressed gas generator, a water supply device, a mixed gas-liquid regulator, and a vaporizer.
  • the mixed gas-liquid regulator mixes water supplied from the water supply device and compressed air supplied from the compressed gas generator to generate an atomized mixed gas-liquid (mist).
  • the discharge part of the mixed gas-liquid regulator is connected to the vaporizer and the atomized mixed gas-liquid (mist) is supplied to the vaporizer.
  • the vaporizer vaporizes the atomized mixed gas-liquid (mist) supplied from the mixed gas-liquid regulator to generate steam.
  • FIG. 4 is a sectional view showing one embodiment of a vaporizer provided in the humidifier.
  • a vaporizer 51 includes a cylindrical outer tube 52 extending from one end 51a to the other end 51b, and a heating element 53 incorporated therein in parallel with the longitudinal direction of the outer tube 52. It is composed of The outer tube 52 is made of a stainless steel cylinder.
  • the heating element 53 includes a heater 54 extending parallel to the longitudinal direction of the outer tube 52. It should be noted that a gap through which the gas-liquid mixture (mist) passes is provided between the inner peripheral surface of the outer tube 52 and the heating element 53.
  • the heater 54 is installed at one outer peripheral end of the outer tubular tube 52 (on the side of the one end 51a of the vaporizer 51) by a connection terminal 54b made of silicon rubber, and receives power supplied from the electric wire 54a. Fever.
  • the surface of the heater 53 that is heated to a high temperature is covered with quartz glass 55.
  • the inner peripheral surface of the outer tube 52 is also coated with quartz glass.
  • the structure of the heater 54 is not particularly limited, and may be a rod-shaped heater or a coil-shaped heater. In addition, it is not always necessary to cover the surface of the heater or the inner peripheral surface of the outer tube with quartz glass. However, in the present embodiment, dust generation is prevented and thermal efficiency is improved.
  • an atomized gas-liquid mixture (mist) supplied from a gas-liquid mixture controller (not shown) is supplied from an inlet 56a opening at one end 51a of the vaporizer 51. It is introduced inside the vaporizer 51.
  • the mixed gas-liquid (mist) introduced into the vaporizer 51 passes through a gap between the outer tube 52 and the heat generator 53 while being heated by the heat generator 53, and opens at the other end 51 b of the vaporizer 51.
  • the discharge port 56b To the discharge port 56b.
  • the water (liquid) in the gas-liquid mixture is heated by the heating element 53 and vaporized to become steam (gas), which is released from the discharge port 56b.
  • the temperature of the steam to be discharged may be controlled while measuring the temperature of the steam at the outlet 56b with a temperature sensor.
  • the air supply device and the exhaust device can be controlled based on the air flow-static pressure curve of the fan motor 32 without controlling the microcomputer.
  • the mixed gas-liquid mixture is heated by the heating element 53 to become high-temperature steam.
  • the temperature of the steam generated in the vaporizer 51 is controlled to 100 ° C. or higher in order to ensure the sterilization state of the steam.
  • the steam generated in the vaporizer 51 is sterilized and can be directly supplied to the inside of the culture chamber 22 without passing through the air filter. Therefore, the sterile environment inside the culture room 22 that maintains the grade A sterile environment is maintained.

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Abstract

L'invention concerne une méthode de contrôle de la pression positive d'un incubateur, par laquelle la température et l'humidité à l'intérieur d'une chambre de culture peuvent être maintenues de manière uniforme, et la pression interne peut être maintenue constamment supérieure à l'environnement externe au moyen d'un mécanisme simple afin de maintenir un environnement aseptique à l'intérieur de la chambre de culture. L'incubateur comprend une chambre de culture, un moyen de régulation de température et un moyen d'humidification. La chambre de culture est pourvue d'un orifice d'alimentation en air et d'un orifice d'évacuation d'air, et l'orifice d'alimentation en air est pourvu d'un moyen d'alimentation en air pour fournir de l'air de l'environnement externe dans la chambre de culture. Comme la zone ouverte de l'orifice d'alimentation en air est plus grande que la zone ouverte de l'orifice d'évacuation d'air, la pression d'air à l'intérieur de la chambre de culture peut être maintenue à une pression statique prédéterminée supérieure à la pression de l'environnement externe en entraînant un ventilateur d'évacuation d'air disposé dans le moyen d'évacuation d'air. Lorsque la pression d'air à l'intérieur de la chambre de culture change en raison de l'humidification par le moyen d'humidification ou analogue, le volume d'air change en fonction de la courbe de pression statique de volume d'air du ventilateur d'évacuation d'air, et l'état de pression positive à l'intérieur de la chambre de culture est maintenu à la pression statique prédéterminée.
PCT/JP2019/022448 2018-07-23 2019-06-06 Méthode de contrôle de pression positive d'incubateur et système de culture l'utilisant WO2020021874A1 (fr)

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
CN112226357A (zh) * 2020-10-27 2021-01-15 江门市恩信生物技术研究有限公司 一种生物培养装置及其方法
CN114606116A (zh) * 2022-03-25 2022-06-10 江苏中慧元通生物科技股份有限公司 一种用于带状疱疹疫苗的培养系统

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JP2017201886A (ja) * 2016-05-09 2017-11-16 株式会社エアレックス インキュベータ
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JP2002098374A (ja) * 2000-09-25 2002-04-05 Takasago Thermal Eng Co Ltd クリーンルームの加湿方法及びクリーンルーム装置
JP2009065844A (ja) * 2007-09-10 2009-04-02 Hitachi Plant Technologies Ltd 細胞調製施設の緊急退避室
WO2015166554A1 (fr) * 2014-04-30 2015-11-05 株式会社エアレックス Système de décontamination
JP2017077208A (ja) * 2015-10-21 2017-04-27 株式会社エアレックス インキュベータ
JP2017201886A (ja) * 2016-05-09 2017-11-16 株式会社エアレックス インキュベータ
WO2018212029A1 (fr) * 2017-05-15 2018-11-22 株式会社エアレックス Incubateur

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112226357A (zh) * 2020-10-27 2021-01-15 江门市恩信生物技术研究有限公司 一种生物培养装置及其方法
CN114606116A (zh) * 2022-03-25 2022-06-10 江苏中慧元通生物科技股份有限公司 一种用于带状疱疹疫苗的培养系统

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