WO2020016638A1 - Procédé d'obtention de produits à valeur ajoutée à partir de son de riz déshuilé - Google Patents

Procédé d'obtention de produits à valeur ajoutée à partir de son de riz déshuilé Download PDF

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Publication number
WO2020016638A1
WO2020016638A1 PCT/IB2018/056666 IB2018056666W WO2020016638A1 WO 2020016638 A1 WO2020016638 A1 WO 2020016638A1 IB 2018056666 W IB2018056666 W IB 2018056666W WO 2020016638 A1 WO2020016638 A1 WO 2020016638A1
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WIPO (PCT)
Prior art keywords
acid
filtrate
solid mass
rice bran
reaction product
Prior art date
Application number
PCT/IB2018/056666
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English (en)
Inventor
Tapas Chatterjee
Original Assignee
Tapas Chatterjee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tapas Chatterjee filed Critical Tapas Chatterjee
Publication of WO2020016638A1 publication Critical patent/WO2020016638A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Definitions

  • the invention relates to a process of obtaining value-added products from de-oiled rice bran.
  • the present invention relates to a process of preparing protein, alcohols, and silicic acid from de-oiled rice bran.
  • Rice bran a by-product produced in a polishing process of brown rice. Till some time back, rice bran was used primarily as an animal feedstock. Rice bran has a very high nutritional value as it contains about 20% of fat and 16-18% of protein, which is almost three times higher in contents compared to white rice or brown rice. Rice bran oil is known to be extracted from rice bran and de-oiled rice bran is obtained as a by-product. As de-oiled rice bran is rich in protein, attempts have been made to extract protein there-from.
  • EP Patent Publication No. 2061339 discloses a process of extracting protein from the de-fatted rice bran that comprises adding 4-8 fold of water by weight, stirring the mixture for 30-60 min to make the particles dispersed uniformly in the mixture by o hydrolysis, and then extract protein by adding 1-5N of NaOH slowly at 20-50 C until the final pH of the mixture is 8-10, generating the protein solution for 30-60 min.
  • the protein solution is centrifuged at more than 5,000g, ambient temperature. And the resultant supernatant becomes containing protein. Then, the supernatant is adjusted to pH 3-5 by adding 1-3N
  • Chinese Patent Application Publication No. CN102150738A discloses a process for preparation of protein from de-oiled rice bran comprising adding sufficient quantity of water to de-oiled rice bran to effect hydration, performing a primary alkali leaching by concentrated NaOH solution with the pH being adjusted to 9.0, and the leaching time being 2 hours and the temperature is adjusted to 60-80°C. Thereafter, a separation step is performed to solid product and a liquid product. The liquid product is subjected to acid precipitation by adding concentrated HC1 with the pH being adjusted to 4.0 and the time period is 2 hours and the temperature is controlled at 40-60°C. Thereafter, the precipitate is separated, washed and neutralized using NaOH with the pH is adjusted to 7.0, and the time is one hour to obtain crude product. The crude product is dehydrated, sterilized and dried to obtain a protein product.
  • the article entitled“Extraction and functional properties of protein from de-oiled rice bran” describes suspending de-oiled rice bran in sodium hydroxide (NaOH) solution (0.05-0.2M) at a ratio of 1:10 (w/v).
  • NaOH sodium hydroxide
  • the suspension was set on a water bath at different temperatures (30-60°C) and different times (60-240 min), with continuous stirring.
  • the suspension was then centrifuged at 6,000 x g for 15 min and filtered using filter paper (Whatman No.l) to separate the insoluble materials.
  • the pH of the supematant/filtrate was adjusted to pH 7 with 1 M hydrochloric acid (HC1). This was then dried in an oven at 50°C for 12 hr to obtain protein.
  • the M.Sc thesis paper entitled“Protein quality of de-oiled rice bran and its use in food products” described a process wherein defatted rice bran sample and distilled deionized water (1:4) was pH adjusted to 9.5 and stirred 30 min at room temperature (RT). The slurry was centrifuged at 5000g for 30 min. (RT). The pH of the supernatant was adjusted to 4.5 and centrifuged again at 5000g for 30 min. The precipitate was washed using water (pH 4.5). The residue was suspended in distilled deionized water (pH 7.0) and freeze- dried overnight to obtain protein.
  • RBPI had similar foaming properties in comparison to egg white. But emulsifying properties of RBPI were significantly lower than those of bovine serum albumin. The result of the amino acid analysis showed that RBPI had a similar profile of essential amino acid requirements for 2-5-year-old children in comparison to that of casein and soy protein isolate.
  • the article entitled“Preparation and characterization of protein hydrolysates from Indian defatted rice bran meal” discloses mixing rice bran meal (through fraction) thoroughly with 10 times distilled water, pH of the medium was then adjusted to 10.0 and was stirred for 1 h at 50-55°C. The slurry was centrifuged at 4000xg for 10 min. The pH of the supernatant liquid was further adjusted to 4.0 with 1N HC1 and again stirred for 1 h at 50-55°C, centrifuged at 8000xg for 20 min to obtain a solid residue (rice bran protein isolate, RPI) which was collected and dried in a vacuum oven at 50°C for 10 h.
  • RPI solid residue
  • the article entitled“Studies on Utilisation of Non-Oil Constituents of Some Oil Bearing Materials” discloses mixing de-oiled rice bran thoroughly with 10 times distilled water, pH of the medium was then adjusted to 10.0 and was stirred for lh at 50- 55°C. The slurry was centrifuged at 4000g for lOmin. The pH of the supernatant liquid was further adjusted to 4.0 with INHC1 and again stirred for lh at50-55°C, centrifuged at 8000g for 20min. The solid residue (RPI) thus obtained was collected and dried in a vacuum oven at 50°C for lOh.
  • Applicant has observed that the need to provide a process for preparing protein which is edible especially for children, still exists. Also, Applicant has felt the need to extract further value-added products from de-oiled rice bran.
  • the process comprises the steps of subjecting the de-oiled rice bran to acid hydrolysis to obtain a first reaction product; separating a first solid mass and a first filtrate from the first reaction product; processing the first solid mass to obtain protein; neutralizing the first filtrate to obtain a second reaction product; separating a second solid mass and a second filtrate from the second reaction product; processing the second solid mass to obtain silicic acid; and processing the second filtrate to obtain alcohols.
  • Figure 1 demonstrates a process for preparing protein, silicic acid and alcohol from de-oiled rice bran in accordance with an embodiment of the invention.
  • the present invention provides a process (100) for preparing protein, silicic acid, and alcohol from de-oiled rice bran.
  • the process (100) comprises subjecting (102) the de-oiled rice bran to acid hydrolysis to obtain a first reaction product.
  • the first reaction product is subjected to a separation process (104) to separate a first solid mass and a first filtrate.
  • the first solid mass is processed (106) to obtain protein.
  • the first filtrate is subjected to a neutralization process (108) to obtain a second reaction product.
  • the second reaction product is subjected to a separation process (110) to obtain a second solid mass and a second filtrate.
  • the second solid mass is processed (112) to obtain silicic acid while the second filtrate is processed (114) to obtain alcohols.
  • subjecting the de-oiled rice bran to acid hydrolysis (102) comprises treating the de-oiled rice bran with an acid, the acid being an inorganic acid selected from a group comprising of hydrochloric acid, sulphuric acid, nitric acid, phosphoric acid, bromic acid, sulfurous acid, thiosulfuric acid, dithionic acid, pyrosulfuric acid, selenic acid, selenious acid, phosphorous acid, hypophosphorous acid, pyrophosphoric acid, polyphosphoric acid, hypophosphoric acid, boric acid, perchloric acid, hypochlorous acid, hydriodic acid, and mixtures thereof.
  • an acid being an inorganic acid selected from a group comprising of hydrochloric acid, sulphuric acid, nitric acid, phosphoric acid, bromic acid, sulfurous acid, thiosulfuric acid, dithionic acid, pyrosulfuric acid, selenic acid, selenious acid, phosphorous
  • subjecting the de-oiled rice bran to acid hydrolysis (102) comprises treating the de-oiled rice bran with hydrochloric acid, nitric acid, and mixture thereof.
  • subjecting the de-oiled rice bran to acid hydrolysis (102) comprises adding hydrochloric acid or nitric acid to de-oiled rice bran to attain pH value in the range of 1.0 to 4.0 and maintaining the same for a time period in the range of 1 to 6 hours at elevated temperature.
  • separating (104) the first solid mass and the first filtrate from the first reaction product comprises subjecting the first reaction product to a solid-liquid separation process such as decanting, filter press, centrifuging, etc.
  • processing (106) the first solid mass to obtain protein comprises:
  • subjecting (116) the first solid mass to alkali hydrolysis to obtain the third reaction product comprises treating the first solid mass with an aqueous solution comprising an alkali at pH of 8.0 to 12.0 at elevated temperature, the alkali being selected from a group comprising of sodium hydroxide, potassium hydroxide, calcium hydroxide, and mixtures thereof.
  • separating (118) the third solid mass and the third filtrate from the third reaction product comprises subjecting the third reaction product to a solid- liquid separation process such as decanting, filter press, centrifuging, etc.
  • acidifying (120) the third filtrate to obtain the fourth reaction product comprises treating the third filtrate with an acid selected from a group comprising hydrochloric acid, nitric acid, and a mixture thereof at pH of 3.0 to 5.5 at elevated temperature.
  • separating (122) the fourth solid mass and the fourth filtrate from the fourth reaction product subjecting the fourth reaction product to a solid- liquid separation process such as decanting, filter press, centrifuging, etc.
  • obtaining (124) protein from the fourth solid mass comprises:
  • the solvent is water or any other suitable solvent.
  • neutralizing (108) the first filtrate to obtain the second reaction product comprises treating the first filtrate at elevated temperature with at least one of sodium hydroxide, potassium hydroxide, calcium hydroxide, and mixtures thereof.
  • separating (110) the second solid mass and the second filtrate from the second reaction product comprises subjecting the second reaction product to a solid-liquid separation process such as decanting, filter press, centrifuging, etc.
  • processing (112) the second solid mass to obtain silicic acid comprises drying the second solid mass to obtain silicic acid.
  • processing (114) the second filtrate to obtain alcohols comprises:
  • the second filtrate comprises Fructo Oligo Saccharides (FOS).
  • FOS Fructo Oligo Saccharides
  • separating (132) the alcohols from the fifth comprises subjecting the fifth filtrate to a rectification column.
  • the fourth filtrate is mixed with the second filtrate and a mixture thereof is processed to obtain alcohols.
  • the protein thus separated from the fifth filtrate is mixed with the fourth solid mass.
  • De-oiled rice bran is obtained and analyzed to understand the constituents thereof.
  • the de oiled rice bran contained roughly 17% protein as determined by protein assay and 10% moisture content.
  • the de-oiled rice bran is subjected to grinding to obtain de-oiled rice bran particles.
  • de-oiled rice bran particles thus obtained of water is added.
  • the ratio of de-oiled rice bran particle to water is in the range of 1:7 to 1:10.
  • hydrochloric acid / nitric acid is added to attain a pH value in the range of 1.0 to 2.5. This mixture is o
  • the acid hydrolyzed product is decanted, to obtain a filtrate and a solid mass.
  • the solid mass contained 30 to 35% of protein and 65% moisture content, while the filtrate has a total solid content of about 5 to 10%.
  • the filtrate obtained from decanting of the acid hydrolyzed product is used for extracting alcohols and silicic acid as will be described later.
  • the alkali hydrolyzed product was decanted to obtain fibers and filtrate.
  • the fibers can be considered a by-product obtained in the process and contained more than 70% moisture content.
  • the fiber thus obtained may be mixed with the de-oiled rice bran particles while in an alternative embodiment, the fiber thus obtained may be used as animal feedstock.
  • the filtrate obtained from decanting of the alkali hydrolyzed product has a total solid content of about 4 to 5 % and is subjected to a precipitation process by adding HC1 solution (to attain a pH of about 4.5 and the temperature of the reaction mass is maintained between 55 to
  • the reaction mass is subjected to a filter press to obtain a wet cake and a filtrate.
  • the wet cake is washed with water and spray dried to obtain rice protein.
  • the filtrate thus obtained after removing the wet cake may be subjected to fermentation to obtain alcohols as will be described later.
  • the filtrate obtained from decanting of the acid hydrolyzed product is subjected to a precipitation process by adding NaOH solution (to attain a pH in the range of about 6 to 7 o
  • the reaction mass is subjected to a filter press to obtain a wet silicic acid and a filtrate.
  • the wet silicic acid is dried at 70 C in a drier to obtain a dry powder of silicic acid.
  • the filtrate thus obtained after removing wet silicic acid has a total solid content of about 4 to 5 %, a protein content of about 15 to 16% and the remaining being constituted by sugars (predominantly Fructo Oligo Saccharides (FOS)).
  • the filtrate thus obtained after removing silicic acid is mixed with the filtrate thus obtained after removing the wet cake and is subjected to the fermentation process, which can be as per prior art.
  • the product obtained from fermentation contains alcohols and some amount of protein.
  • the product obtained from fermentation is subjected to rectification, which may be performed as per prior art, to obtain alcohols and protein.
  • the protein thus obtained may be washed with water and spray dried to obtain rice protein.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne un procédé d'obtention de produits à valeur ajoutée à partir de son de riz déshuilé. En particulier, la présente invention concerne un procédé de préparation d'une protéine, d'alcools et d'acide silicique à partir de son de riz déshuilé. Le procédé de préparation de protéines, d'acides siliciques et d'alcools à partir de son de riz déshuilé comprend la soumission du son de riz déshuilé à une hydrolyse acide pour obtenir un premier produit de réaction; la séparation d'une première masse solide et d'un premier filtrat du premier produit de réaction; le traitement de la première masse solide pour obtenir des protéines; la neutralisation du premier filtrat pour obtenir un second produit de réaction; la séparation d'une seconde masse solide et d'un second filtrat du second produit de réaction; le traitement de la seconde masse solide pour obtenir des acides siliciques; et le traitement du second filtrat pour obtenir des alcools.
PCT/IB2018/056666 2018-07-16 2018-08-31 Procédé d'obtention de produits à valeur ajoutée à partir de son de riz déshuilé WO2020016638A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201811026550 2018-07-16
IN201811026550 2018-07-16

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WO2020016638A1 true WO2020016638A1 (fr) 2020-01-23

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5620877A (en) * 1993-03-26 1997-04-15 Arkenol, Inc. Method of fermenting sugars resulting from strong acid hydrolysis
US5735916A (en) * 1995-07-13 1998-04-07 Lucas; James Lewis Process for production of lignin fuel, ethyl alcohol, cellulose, silica/silicates, and cellulose derivatives from plant biomass
US5782982A (en) * 1993-03-26 1998-07-21 Arkenol, Inc. Method of removing silica or silicates from solids resulting from the strong acid hydrolysis of cellulosic and hemicellulosic materials
EP2061339A1 (fr) * 2007-09-12 2009-05-27 CJ Cheiljedang Corporation Procédé de préparation de concentré de protéines à partir de son de riz

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5620877A (en) * 1993-03-26 1997-04-15 Arkenol, Inc. Method of fermenting sugars resulting from strong acid hydrolysis
US5782982A (en) * 1993-03-26 1998-07-21 Arkenol, Inc. Method of removing silica or silicates from solids resulting from the strong acid hydrolysis of cellulosic and hemicellulosic materials
US5735916A (en) * 1995-07-13 1998-04-07 Lucas; James Lewis Process for production of lignin fuel, ethyl alcohol, cellulose, silica/silicates, and cellulose derivatives from plant biomass
EP2061339A1 (fr) * 2007-09-12 2009-05-27 CJ Cheiljedang Corporation Procédé de préparation de concentré de protéines à partir de son de riz

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