WO2020009538A1 - Composition for skin health including extract of seed of new soybean cultivar scel-1 - Google Patents
Composition for skin health including extract of seed of new soybean cultivar scel-1 Download PDFInfo
- Publication number
- WO2020009538A1 WO2020009538A1 PCT/KR2019/008302 KR2019008302W WO2020009538A1 WO 2020009538 A1 WO2020009538 A1 WO 2020009538A1 KR 2019008302 W KR2019008302 W KR 2019008302W WO 2020009538 A1 WO2020009538 A1 WO 2020009538A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- composition
- skin
- extract
- seed
- cultivar
- Prior art date
Links
- 244000068988 Glycine max Species 0.000 title claims abstract description 62
- 239000000284 extract Substances 0.000 title claims abstract description 58
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 55
- 239000000203 mixture Substances 0.000 title claims description 78
- 230000036559 skin health Effects 0.000 title claims description 17
- XFZJEEAOWLFHDH-NFJBMHMQSA-N procyanidin B2 Chemical compound C1([C@@H]2[C@H](O)[C@H](C3=C(O)C=C(O)C=C3O2)C=2C(O)=CC(O)=C3C[C@H]([C@H](OC3=2)C=2C=C(O)C(O)=CC=2)O)=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-NFJBMHMQSA-N 0.000 claims description 44
- 230000037303 wrinkles Effects 0.000 claims description 36
- 238000000605 extraction Methods 0.000 claims description 25
- 150000003839 salts Chemical class 0.000 claims description 24
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 claims description 22
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 claims description 22
- 229920002350 Procyanidin B2 Polymers 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 21
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 claims description 20
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 claims description 20
- 235000012734 epicatechin Nutrition 0.000 claims description 20
- RKWHWFONKJEUEF-GQUPQBGVSA-O Cyanidin 3-O-glucoside Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC2=C(O)C=C(O)C=C2[O+]=C1C1=CC=C(O)C(O)=C1 RKWHWFONKJEUEF-GQUPQBGVSA-O 0.000 claims description 18
- YTMNONATNXDQJF-UBNZBFALSA-N chrysanthemin Chemical compound [Cl-].O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC2=C(O)C=C(O)C=C2[O+]=C1C1=CC=C(O)C(O)=C1 YTMNONATNXDQJF-UBNZBFALSA-N 0.000 claims description 18
- 239000004480 active ingredient Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- -1 C1-C6 alcohol Substances 0.000 claims description 10
- 239000002537 cosmetic Substances 0.000 claims description 5
- 230000006872 improvement Effects 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 4
- 239000008194 pharmaceutical composition Substances 0.000 claims description 3
- 230000002265 prevention Effects 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 32
- 230000000694 effects Effects 0.000 description 17
- 201000004624 Dermatitis Diseases 0.000 description 15
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 9
- 244000046052 Phaseolus vulgaris Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 235000013339 cereals Nutrition 0.000 description 8
- 239000002904 solvent Substances 0.000 description 8
- 235000020712 soy bean extract Nutrition 0.000 description 8
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 108010035532 Collagen Proteins 0.000 description 6
- 102000008186 Collagen Human genes 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 229920001436 collagen Polymers 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 238000011580 nude mouse model Methods 0.000 description 6
- 108010050808 Procollagen Proteins 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 3
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 3
- 241000913743 Rhynchosia Species 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 238000012790 confirmation Methods 0.000 description 3
- 239000000287 crude extract Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000002552 dosage form Substances 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 239000000469 ethanolic extract Substances 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000013355 food flavoring agent Nutrition 0.000 description 3
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 238000007911 parenteral administration Methods 0.000 description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
- 229920000053 polysorbate 80 Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000003642 reactive oxygen metabolite Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-3',4',5,7-Tetrahydroxy-2,3-trans-flavan-3-ol Natural products C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 2
- 229930013783 (-)-epicatechin Natural products 0.000 description 2
- 235000007355 (-)-epicatechin Nutrition 0.000 description 2
- XMIIGOLPHOKFCH-UHFFFAOYSA-N 3-phenylpropionic acid Chemical compound OC(=O)CCC1=CC=CC=C1 XMIIGOLPHOKFCH-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 2
- 238000008157 ELISA kit Methods 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 2
- 239000004909 Moisturizer Substances 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 2
- 241000289690 Xenarthra Species 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000000174 gluconic acid Substances 0.000 description 2
- 235000012208 gluconic acid Nutrition 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 2
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical compound OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 230000001333 moisturizer Effects 0.000 description 2
- TXXHDPDFNKHHGW-UHFFFAOYSA-N muconic acid Chemical compound OC(=O)C=CC=CC(O)=O TXXHDPDFNKHHGW-UHFFFAOYSA-N 0.000 description 2
- 239000002674 ointment Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000006201 parenteral dosage form Substances 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- QBYIENPQHBMVBV-HFEGYEGKSA-N (2R)-2-hydroxy-2-phenylacetic acid Chemical compound O[C@@H](C(O)=O)c1ccccc1.O[C@@H](C(O)=O)c1ccccc1 QBYIENPQHBMVBV-HFEGYEGKSA-N 0.000 description 1
- MIOPJNTWMNEORI-GMSGAONNSA-N (S)-camphorsulfonic acid Chemical compound C1C[C@@]2(CS(O)(=O)=O)C(=O)C[C@@H]1C2(C)C MIOPJNTWMNEORI-GMSGAONNSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 description 1
- AMMPLVWPWSYRDR-UHFFFAOYSA-N 1-methylbicyclo[2.2.2]oct-2-ene-4-carboxylic acid Chemical compound C1CC2(C(O)=O)CCC1(C)C=C2 AMMPLVWPWSYRDR-UHFFFAOYSA-N 0.000 description 1
- SGTNSNPWRIOYBX-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile Chemical compound C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 SGTNSNPWRIOYBX-UHFFFAOYSA-N 0.000 description 1
- UPHOPMSGKZNELG-UHFFFAOYSA-N 2-hydroxynaphthalene-1-carboxylic acid Chemical compound C1=CC=C2C(C(=O)O)=C(O)C=CC2=C1 UPHOPMSGKZNELG-UHFFFAOYSA-N 0.000 description 1
- MLMQPDHYNJCQAO-UHFFFAOYSA-N 3,3-dimethylbutyric acid Chemical compound CC(C)(C)CC(O)=O MLMQPDHYNJCQAO-UHFFFAOYSA-N 0.000 description 1
- XLZYKTYMLBOINK-UHFFFAOYSA-N 3-(4-hydroxybenzoyl)benzoic acid Chemical compound OC(=O)C1=CC=CC(C(=O)C=2C=CC(O)=CC=2)=C1 XLZYKTYMLBOINK-UHFFFAOYSA-N 0.000 description 1
- BMYNFMYTOJXKLE-UHFFFAOYSA-N 3-azaniumyl-2-hydroxypropanoate Chemical compound NCC(O)C(O)=O BMYNFMYTOJXKLE-UHFFFAOYSA-N 0.000 description 1
- ZRPLANDPDWYOMZ-UHFFFAOYSA-N 3-cyclopentylpropionic acid Chemical compound OC(=O)CCC1CCCC1 ZRPLANDPDWYOMZ-UHFFFAOYSA-N 0.000 description 1
- MOMKYJPSVWEWPM-UHFFFAOYSA-N 4-(chloromethyl)-2-(4-methylphenyl)-1,3-thiazole Chemical compound C1=CC(C)=CC=C1C1=NC(CCl)=CS1 MOMKYJPSVWEWPM-UHFFFAOYSA-N 0.000 description 1
- RJWBTWIBUIGANW-UHFFFAOYSA-N 4-chlorobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=C(Cl)C=C1 RJWBTWIBUIGANW-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- WWZKQHOCKIZLMA-UHFFFAOYSA-N Caprylic acid Natural products CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 1
- 241001107116 Castanospermum australe Species 0.000 description 1
- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 description 1
- ACFGRWJEQJVZTM-LEJBHHMKSA-L Magnesium L-ascorbic acid-2-phosphate Chemical compound [Mg+2].OC[C@H](O)[C@H]1OC(=O)C(OP([O-])([O-])=O)=C1O ACFGRWJEQJVZTM-LEJBHHMKSA-L 0.000 description 1
- 102000005741 Metalloproteases Human genes 0.000 description 1
- 108010006035 Metalloproteases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- TXXHDPDFNKHHGW-CCAGOZQPSA-N Muconic acid Natural products OC(=O)\C=C/C=C\C(O)=O TXXHDPDFNKHHGW-CCAGOZQPSA-N 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- IWYDHOAUDWTVEP-UHFFFAOYSA-N R-2-phenyl-2-hydroxyacetic acid Natural products OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 102100033121 Transcription factor 21 Human genes 0.000 description 1
- 101710119687 Transcription factor 21 Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 229960000271 arbutin Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- 229940092714 benzenesulfonic acid Drugs 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- GONOPSZTUGRENK-UHFFFAOYSA-N benzyl(trichloro)silane Chemical compound Cl[Si](Cl)(Cl)CC1=CC=CC=C1 GONOPSZTUGRENK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000021279 black bean Nutrition 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229960001948 caffeine Drugs 0.000 description 1
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 235000013985 cinnamic acid Nutrition 0.000 description 1
- 229930016911 cinnamic acid Natural products 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000015140 cultured milk Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- AFAXGSQYZLGZPG-UHFFFAOYSA-N ethanedisulfonic acid Chemical compound OS(=O)(=O)CCS(O)(=O)=O AFAXGSQYZLGZPG-UHFFFAOYSA-N 0.000 description 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 230000006355 external stress Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000011087 fumaric acid Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- LBQIJVLKGVZRIW-ZDUSSCGKSA-N glabridin Chemical compound C1([C@H]2CC3=CC=C4OC(C=CC4=C3OC2)(C)C)=CC=C(O)C=C1O LBQIJVLKGVZRIW-ZDUSSCGKSA-N 0.000 description 1
- 229940093767 glabridin Drugs 0.000 description 1
- PMPYOYXFIHXBJI-ZDUSSCGKSA-N glabridin Natural products C1([C@H]2CC=3C=CC4=C(C=3OC2)CCC(O4)(C)C)=CC=C(O)C=C1O PMPYOYXFIHXBJI-ZDUSSCGKSA-N 0.000 description 1
- LBQIJVLKGVZRIW-UHFFFAOYSA-N glabridine Natural products C1OC2=C3C=CC(C)(C)OC3=CC=C2CC1C1=CC=C(O)C=C1O LBQIJVLKGVZRIW-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 239000001685 glycyrrhizic acid Substances 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 229940069445 licorice extract Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 229960002510 mandelic acid Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000008204 material by function Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N n-hexanoic acid Natural products CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- KVBGVZZKJNLNJU-UHFFFAOYSA-N naphthalene-2-sulfonic acid Chemical compound C1=CC=CC2=CC(S(=O)(=O)O)=CC=C21 KVBGVZZKJNLNJU-UHFFFAOYSA-N 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 235000019449 other food additives Nutrition 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- IUGYQRQAERSCNH-UHFFFAOYSA-N pivalic acid Chemical compound CC(C)(C)C(O)=O IUGYQRQAERSCNH-UHFFFAOYSA-N 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- WSHYKIAQCMIPTB-UHFFFAOYSA-M potassium;2-oxo-3-(3-oxo-1-phenylbutyl)chromen-4-olate Chemical compound [K+].[O-]C=1C2=CC=CC=C2OC(=O)C=1C(CC(=O)C)C1=CC=CC=C1 WSHYKIAQCMIPTB-UHFFFAOYSA-M 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000003044 randomized block design Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000011083 sodium citrates Nutrition 0.000 description 1
- 235000019983 sodium metaphosphate Nutrition 0.000 description 1
- 235000019830 sodium polyphosphate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- 230000037317 transdermal delivery Effects 0.000 description 1
- SOBHUZYZLFQYFK-UHFFFAOYSA-K trisodium;hydroxy-[[phosphonatomethyl(phosphonomethyl)amino]methyl]phosphinate Chemical compound [Na+].[Na+].[Na+].OP(O)(=O)CN(CP(O)([O-])=O)CP([O-])([O-])=O SOBHUZYZLFQYFK-UHFFFAOYSA-K 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 229960001722 verapamil Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Definitions
- compositions for skin health including an extract of a seed of new soybean cultivar SCEL-1
- Soybeans are an abundant crop source of vegetable proteins, and contain not only proteins, but also a variety of excellent functional materials including unsaturated fatty acids, amino acids, isoflavones, and phenolic acids.
- soybeans are being used as a protein source to replace animal proteins.
- One or more embodiments include a composition for skin health, the composition including, as an active ingredient, an extract obtained by performing an extraction process on a seed of soybean Glycine max (L.) Merrill cultivar SCEL-1 by using water, C 1 -C 6 alcohol, or a mixture thereof, wherein the seed contains cyanidin-3-O-glucoside, procyanidin B2, and epicatechin, wherein an amount of the procyanidin B2 is greater than that of the cyanidin-3-O-glucoside, and a representative sample of the seed is deposited with the Korean Agricultural Culture Collection(KACC), which is an International Depository Authority(IDA) under the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure under the accession number KACC 88002BP on November 8, 2018.
- KACC Korean Agricultural Culture Collection
- One or more embodiments include a composition for skin health, the composition including, as an active ingredient, cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof, at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48 on a weight basis.
- One or more embodiments include a method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, or preventing occurrence of skin inflammation in a subject or improving skin inflammation in a subject, the method including administrating the composition to a subject.
- a first aspect of the present disclosure provides a composition for skin health, the composition including, as an active ingredient, an extract obtained by performing an extraction process on a seed of soybean Glycine max (L.) Merrill cultivar SCEL-1 by using water, C 1 -C 6 alcohol, or a mixture thereof, wherein the seed contains cyanidin-3-O-glucoside, procyanidin B2, and epicatechin, wherein an amount of the procyanidin B2 is greater than that of the cyanidin-3-O-glucoside, and a representative sample of the seed is deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018.
- cyanidin-3-O-glucoside and procyanidin B2 may be contained at a ratio of 1: 0.8 or more, 1: 0.8 to 5.0, 1: 0.8 to 3.0, 1: 0.8 to 2.5, or 1: 0.8 to 2.1, on a weight basis.
- cyanidin-3-O-glucoside and procyanidin B2 may be contained at a ratio of 1: 2.0 or more, 1: 2.0 to 5.0, 1: 2.0 to 3.0, 1: 2.0 to 2.5, or 1: 2.0 to 2.1, on a weight basis.
- amount as used herein may be calculated based on an extract obtained by pulverizing the seed and incubating the pulverized seed at a temperature in a range of about 25 °C to about 55 °C for 6 hours by using 70 (v/v)% aqueous ethanol.
- amount as used herein is described by way of example, and should be understood as being variable depending on soil, climate conditions, and an individual seed that are considered for production of a plant body of a seed.
- a total amount of procyanidin B2 and epicatechin may be, on a weight basis, twice or more or 2.5 times or more, for example, 2.6 times to 5.1 times greater than that of a cultivar named Wonheug, which is a standard cultivar.
- a total amount of cyanidine-3-O-glucoside, procyanidine B2, and epicatechin in the extract may be in a range of about 2.1 % to about 3.4 % based on a total weight of the extract, and a total amount of procyanidine B2 and epicatechin in the extract may be in a range of about 1.31 % to about 2.4 % based on a total weight of the extract.
- cyanidin-3-O-glucoside, procyanidin B2, and epicatechin may be contained at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48 on a weight basis.
- an amount of the extract may be in a range of about 0.001 % to about 99.9 %, about 0.005 % to about 99.9 %, about 0.01 % to about 80.0 %, about 0.01 % to about 60.0 %, about 0.01 % to about 50.0 %, about 0.01 % to about 30.0 %, about 0.01 % to about 20.0 %, about 0.01 % to about 15.0 %, about 0.01 % to about 10.0 %, about 0.01 % to about 5.0 %, about 0.1% to about 99.9 %, about 1.0 % to about 80.0 %, about 5.0 % to about 60.0 %, about 5.0 % to about 50.0 %, about 5.0 % to about 30.0 %, about 30.0% to about 50.0 %, about 40.0% to about 80.0 %, about 15.0 % to about 70.0 %, or about 50.0% to about 90.0 %, based on a total
- the characteristics of the soybean cultivar may be measured and verified according to "The guidelines for investigation of characteristics of each crop for examination of new varieties: Soybean Glycine max (L.) Merrill (Korea Seed Variety Service (KSVS) of the Ministry of Agriculture, Food and Rural Affairs (MAFRA), 2014: http.//www.seed.go.kr)", wherein the guidelines determine matters necessary to explain the characteristics of the varieties for each crop in Annex 1 in Article 2 of the Seed Management Guidelines pursuant to Article 30 of the Act of Protection of New Varieties of Plants and Article 33 of the Enforcement Decree of the same Act, and the directions for the qualification tests necessary for cultivation examination according to Article 47 of Enforcement Regulation of the same Act.
- quantitative traits among the characteristics of the soybean cultivar are represented as mean values.
- composition may not include any extract or active ingredient other than the extract described above, and in this regard, the extract described above may be included as the only extract or active ingredient.
- a second aspect of the present disclosure provides a composition for skin health, the composition including, as an active ingredient, cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48 on a weight basis.
- an amount of each of cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof may be in a range of about 0.001% to about 99.9 %, about 0.005% to about 99.9 %, about 0.01% to about 80.0 %, about 0.01% to about 60.0 %, about 0.01% to about 50.0 %, about 0.01% to about 30.0 %, about 0.01 % to about 20.0 %, about 0.01 % to about 15.0 %, about 0.01 % to about 10.0 %, about 0.01 % to about 5.0 %, about 0.1% to about 99.9 %, about 1.0 % to about 80.0 %, about 5.0 % to about 60.0 %, about 5.0 % to about 50.0 %, about 5.0 % to about 30.0 %, about 30.0% to about 50.0 %, about 40
- composition of each of the first aspect and the second aspect of the present disclosure may be food, a cosmetic composition, or a pharmaceutical composition.
- physiologically acceptable salt thereof as used herein includes a meaning of "pharmaceutically acceptable salt thereof".
- pharmaceutically acceptable salt thereof refers to possible use on animals, more particularly, to humans, without having significant toxic effects when used in a typical medicinal dosage. This expression infers that, for example, one that can be or is approved by a government or regulatory organization equivalent thereto, or one that is listed in the pharmacopoeia or recognized in other general pharmacopoeias.
- the expression "pharmaceutically acceptable salt thereof” refers to a salt according to an embodiment of the present disclosure being pharmaceutically acceptable and having pharmacological activity of a parent compound.
- a salt may include: (1) an acid addition salt formed of an inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, or the like; or an organic acid, such as acetic acid, propionic acid, hexanoic acid, cyclopentane propionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, 3-(4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid, 2-hydroxyethanesulfonic acid
- active ingredient refers to an ingredient which exhibits a desired activity alone or an ingredient capable of exhibiting activity with a carrier that is not active by itself.
- the expression "for skin health” refers to preventing occurrence of skin wrinkles or improving skin wrinkles, or preventing skin inflammation or improving skin inflammation, wherein a cause of the skin wrinkles or the skin inflammation may include external stress, such as ultraviolet rays or oxidizing agents on the skin; or internal stress.
- the composition may be also able to prevent or treat skin wrinkles and/or skin inflammation.
- the composition may include, as an active ingredient, an extract obtained by performing an extraction process on a soybean cultivar seed by using water, C 1 -C 6 alcohol, or a mixture thereof, wherein a representative sample of the soybean cultivar seed is deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018.
- KACC Korean Agricultural Culture Collection
- the composition may be one that is effective for skin health, and more particularly, may be one that is effective for skin damage caused by ultraviolet rays or oxidative stress.
- the composition When the composition of the present disclosure is applied to medicines, the composition may be formulated into a dosage form in a solid, semi-solid, or liquid form for oral or parenteral administration by adding a commonly used inorganic or organic carrier thereto which includes the composition as an active ingredient.
- Examples of the dosage form for oral administration may include tablets, pills, granules, capsules, powders, infinitesimal grains, emulsions, syrup, pellets, and the like.
- examples of the dosage form for parenteral administration may include injections, instillations, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like.
- the active ingredient may be easily formulated according to the conventional method, and a surfactant, an excipient, a coloring agent, a flavoring agent, a preservative, a stabilizer, a buffer, a suspension, and other common adjuvants may be used as appropriate.
- composition according to the present disclosure may be administered orally, parenterally, rectally, topically, transdermally, intravascularly, intramuscularly, intraperitoneally, subcutaneously, and the like.
- a dosage of the active ingredient may vary depending on the age, gender, and weight of a subject to be treated, a particular disease or pathological condition to be treated, the severity of a disease or pathological condition, a route of administration, and a determination made by a prescriber. The determination of the dosage based on these factors may be within a level of one of ordinary skill in the art, and the dosage may be in a range of about 0.001 mg/day to about 2,000 mg/kg/day, more particularly, a range about 0.5 mg/kg/day to about 1,500 mg/kg/day.
- the composition may include a health food composition.
- the composition may be processed into a drink, fermented milk, a cheese, a yogurt, a juice, a probiotic agent, and a health supplement, each including the composition.
- the composition may be used in a variety of other food additives.
- the composition may contain other components that exhibit a synergistic effect with main effects within a range that does not damage the intended main effects of the present disclosure.
- the composition may further include an additive, such as a flavoring agent, a dye, a bactericide, an antioxidant, a preservative, a moisturizer, an instillation, an inorganic salt, an emulsifier, or a synthetic polymer.
- the composition may further include an adjuvant component, such as a water-soluble vitamin, an oil-soluble vitamin, a polymeric peptide, a polymeric polysaccharide, or a seaweed extract.
- the components above may be appropriately selected and mixed by one of ordinary skill in the art without difficulty depending on the formulation and purpose of use, and an amount of the components to be added may be selected within a range that does not damage the objects and effects of the present disclosure.
- composition of the present disclosure may be in various forms, such as a solution, an emulsion, a viscous mixture, a tablet, a powder, and the like, and may be administered by various methods using a simple drink, an injection, a spray, or a squeezer.
- the composition may include a cosmetic composition, and may be formulated in a parenteral dosage form.
- An example of the parenteral dosage form may include an injection or an external skin application, and examples of the external skin application may include a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or any combination thereof.
- components such as typical cosmetics or medicines used for external skin applications, for example, an aqueous component, an oily component, a powder component, an alcohol component, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, an antiseptic, an antioxidant, a surfactant, a flavoring agent, a dye, various skin nutrients, or any combination thereof, may be appropriately mixed as needed.
- a chelating agent such as disodium edentate, trisodium edentate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, and the like; caffeine, tannin, verapamil, licorice extract, glabridin, hot water extract of calines from fruit, and various herb medicines; medicines such as tocopherol acetate, glycyrrhizic acid, tranexamic acid, or a derivative or salt of any of the foregoing; vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, and kojic acid; and sugars such as glucose, fructose, and trehalose, may be mixed as appropriate.
- a chelating agent such as disodium edentate, trisodium edentate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, and the like
- caffeine tannin, verapamil, licorice extract, glabridin,
- the extract of the soybean cultivar SCEL-1 may be obtained by performing an extraction process on the soybean cultivar SCEL-1 by using water, C 1 -C 6 alcohol, or a mixture thereof.
- the extract of the soybean SCEL-1 may be obtained by using methanol, ethanol, propanol, butanol, pentanol, or hexanol.
- the extract of the soybean cultivar SCEL-1 of the present disclosure may include a crude extract obtained by performing an extraction process thereon by using a solvent, such as water, C 1 -C 6 alcohol, or a mixture thereof.
- a solvent such as water, C 1 -C 6 alcohol, or a mixture thereof.
- the C 1 -C 6 alcohol may be, for example, methanol or ethanol.
- a volume of the solvent may be about 2 times to about 15 times, about 3 times to about 15 times, about 5 times to about 15 times, or about 10 times greater than the soybean.
- the extraction process may include heat extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction, and there is no limitation as long as the extraction process is obvious to one of ordinary skill in the art.
- the extraction process may be performed at room temperature, but for better efficient extraction, the extraction process may be performed under warm conditions.
- the extraction process may be performed at a temperature in a range of about 40 °C to about 100 °C, about 40 °C to about 80 °C, about 40 °C to about 60 °C, or at a temperature of about 50 °C.
- the time for which the extraction process is performed may be in a range of about 2 hours to about 8 hours, about 4 hours to about 8 hours, about 5 hours to about 7 hours, about 5.5 hours to about 6.5 hours, or may be about 6 hours.
- the temperature may vary depending on conditions including a solvent used for the extraction process.
- the extraction process may be performed one or more times. For example, an extract obtained by combining all the extracts from the extraction process performed one to 5 times, one to 4 times, or 3 times consecutively may be used.
- the extract of the soybean cultivar SCEL-1 of the present disclosure may include a crude extract of the soybean cultivar SCEL-1, and may be contained as a water-soluble fraction of the organic solvent obtained by further extraction performed on the crude extract.
- the organic solvent may include hexane, methylene chloride, ethyl acetate, n-butanol, and the like.
- the extract or the water-soluble fraction thereof may be used as it is.
- the extract may be used as a concentrate obtained by concentration, and in one or more embodiments, the extract may be used in a lyophilized form obtained by concentration followed by lyophilization.
- a third aspect of the present disclosure provides a method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, or preventing skin inflammation in a subject or improving skin inflammation in a subject, the method including administrating the composition of the first or second aspect to a subject.
- the administrating may be oral or parenteral administration.
- the method according to an aspect of the present disclosure may be able to prevent occurrence of skin wrinkles in a subject or to improve skin wrinkles in a subject.
- the method according to an aspect of the present disclosure may be also to prevent occurrence of skin inflammation in a subject or to improve skin inflammation in a subject.
- the method of the present disclosure may include administrating the composition to the skin of a subject.
- the administrating may include coating or applying the composition to the skin.
- the method of the present disclosure may be considered as a make-up method, and may prevent or reduce the occurrence of reactive oxygen species (ROS) in the skin.
- ROS reactive oxygen species
- the ROS may be produced when ultraviolet rays are irradiated to the skin.
- the subject may include a mammal including a vertebrate, or fish.
- the vertebrate may include a cow, a pig, a cat, a dog, or a sheep. That is, the subject may be a mammal other than a human.
- FIG. 1 is a diagram showing effects of an extract of a selected from soybean cultivar on the improvement of wrinkles of the skin damaged by ultraviolet rays in a nude mouse;
- FIG. 2 shows the results of staining a tail tissue derived from a nude mouse irradiated with ultraviolet rays
- FIG. 3 shows the results of the H&E staining performed on the mouse skin irradiated with ultraviolet rays and effects of an extract of a soybean on the collagen density in the mouse skin.
- soybean seed grains which are black flat-shaped granules collected as genetic resources from Idong-eup, Yongin-si, Gyeonggi-do, Korea, were prepared.
- the traits of the soybean seeds did not seem to be fixed, and thus, the soybean seeds were not recognized as species due to lack of stability and uniformity.
- the pure line selection was carried out from 2013 to 2017, and more particularly, it was carried out by the whole crop experimental field in the Rural Development Administration (RDA): National Institute of Crop Science (NICS) located in Suwon, Republic of Korea from 2013 to 2014, and then, in the field located in Iseo-myeon, Wanju-gun, Republic of Korea from 2015 to 2017.
- RAA Rural Development Administration
- NIS National Institute of Crop Science
- a specific process of the pure line selection from the collected genetic resources of the soybean is as follows: as genetic resources, 20 soybean seeds were sown at the beginning of June every year from 2013 to 2016 in the experimental field affiliated with the RDA; then, 1 seed was selected for the purity improvement at the end of November and then harvested as a sowing seed for the following year. After four years of the pure line selection process, one finally fixed pure line seed was selected.
- Source Growth type (determinate, indeterminate) Number of days to flowering Flower color (violet or white) Hypocotyl color (violet or green) Trichome color(brown, light gray) Testa Hilum color 100-seed weight(g) Leaf shape Wonheug (standard) Determinate 66 Violet Violet Brown Black Black 11.6 Globular Cheongja-3 (control) Determinate 70 Violet Violet Brown Black Black 40.7 Globular Selected cultivar (SCEL-1) Indeterminate 58 White Green Brown Black Black 8.8 Oblique ellipse
- the performance of each cultivar was identified by repeating the randomized block design three times by using the Wonheug bean as a standard cultivar and Cheongja-3 as a control cultivar in the field affiliated with the NISC in summer of 2017.
- the agronomic traits of each cultivar were examined by cultivating seeds after sowing two seed grains per row in an experimental plot (60 cm X 15 cm) containing four rows (4 meters long) on June 8, 2017, and then cultivating the seeds.
- the Wonheug bean (Cultivar Application Publication Number: 2010-341) is a microcarpa bean cultivar with a black seed coat developed by the NICS in 2009, and is currently the most cultivated cultivar in farming.
- the Wonheug bean is similar to the selected cultivar SCEL-1, and thus, may be used as a standard cultivar for the comparison.
- the control cultivar, Cheongja-3 (Cultivar Application Publication Number: 2005-176), was developed by the NICS in 2004 and is a soybean cultivar that has a black seed coat, is large-sized, and is used for cooking with rice.
- the Wonheug bean and Cheongja-3 are commercially available from the Korean Seed and Variety Service (KSVS) or the like.
- the agronomic traits and traits examined for the performance testing were examined mainly in terms of yield component traits, such as stem length, number of nodes, number of branches, and the like.
- yield component traits such as stem length, number of nodes, number of branches, and the like.
- 10 plants with repeated nodes were examined during a maturation period in terms of stem length, number of nodes, number of branches, number of pods, 100-seed weight, yield per plant, yield per area, and yield per 10 acres (a).
- Tables 2 and 3 show the characteristics of the selected soybean cultivar, the standard cultivar, and the control cultivar.
- Source Length of stem (cm) Number of nodes Number of branches Seed number per pod 1 pod 2 pods 3 pods Wonheug (standard) 56.6 ⁇ 4.3 14.7 ⁇ 1.7 6.7 ⁇ 2.1 18.8 ⁇ 2.3 201 ⁇ 21 32 ⁇ 15.2 Cheongja-3 (control) 71.9 ⁇ 2.7 14.1 ⁇ 0.3 7.3 ⁇ 0.6 13.8 ⁇ 4.9 81 ⁇ 12 4.7 ⁇ 0.8 Selected cultivar (SCEL-1) 82.9 ⁇ 1.4 15.8 ⁇ 0.5 6.4 ⁇ 0.3 12.9 ⁇ 2.3 133 ⁇ 44 38.3 ⁇ 8.6
- the selected cultivar had 184 pods, 397 grains, 23.1 g of the yield per plant, and 4.6 g of the yield per area, wherein the resulting numbers and yields were lower than those of the standard cultivar.
- 100 grains were weighed at 9.4 g, and in this regard, the selected cultivar had small grains as compared with the standard cultivar.
- the yield per 10a was calculated based on the results above, the selected cultivar showed 413 kg/10 a, which was only about 70% of the yield per 10a of the standard cultivar, Wonheug.
- procyanidin B2 and epicatechin which are functional substances, were about 301% and 217%, respectively.
- the amount of procyanidin B2 was 149.4 ug/100 mg, 49.5 ug/100 mg, and 34.3 ug/100 mg in the selected cultivar SCEL-1, Wonheug, and Cheongja-3, respectively.
- the amount of epicatechin was 46.9 ug/100 mg, 21.6 ug/100 mg, and 20.1 ug/100 mg in the selected cultivar SCEL-1, Wonheug, and Cheongja-3, respectively.
- the amounts of procyanidin B2 and epicatechin were confirmed by an extraction process performed according to the method of Section 1 in Example 2.[0016] According to the experiments, the cultivar SCEL-1 was found to have high uniformity and was distinguished from the standard cultivar and the existing cultivars by the comparison, and was also able to be recognized as an independent cultivar with distinct characteristics in terms of the agronomic traits, the yield ability, and the functional material contents.
- FIG. 1 shows a schematic breeding diagram of a soybean cultivar according to the present disclosure.
- Example 2 Extract obtained by performing an extraction process on a newly selected soybean seed by using water, alcohol, or a mixture, and use of the extract
- the seed was pulverized by using a high-speed pulverizer (Wonder Blender, 820W, 30000 RPM, Sanplatec Corp) to prepare powdered seed.
- a high-speed pulverizer Wood Blender, 820W, 30000 RPM, Sanplatec Corp
- 1 g of the powdered seed thus obtained was mixed with 100 ml of 70 (v/v)% aqueous ethanol in a glass tube, and the mixed solution was stirred at a temperature of 50 °C for 6 hours by using a magnetic bar.
- the resulting extract was filtered through filter paper (Garde No.131 Qualitative filter paper, Advantec).
- the ethanol layer was dried in a nitrogen gas drier (hurricane-Eagle, Chongmin Technology) for 1 hour, and then, was lyophilized for one day to completely remove moisture,thereby obtaining 0.19 g (standard deviation SD: ⁇ 0.015) of a soybean extract.
- a nitrogen gas drier hurricane-Eagle, Chongmin Technology
- it was dissolved at a concentration of 30 mg/ml in 50% v/v ethanol, and was subjected to the HPLC analysis.
- the EpiDermFT TM 3D skin product manufactured by MatTek Company was purchased and used in this experiment. After a 6-well plate was filled with 900 ul of the medium provided by the manufacturer, the prepared 3D skin product was placed on each well of the plate, and then, cultured in an incubator under the conditions of 37 °C, 5 % CO 2 , and 100 % humidity.
- UVB ultraviolet B
- the medium in each cell was replaced with a medium containing test materials in an amount of 10 ug/ml, 30 ug/ml, and 100 ug/ml, respectively.
- the culture medium at the bottom i.e., the culture medium containing collagenase (e.g., MMP-1) and procollagen that were secreted from the artificial skin product was harvested.
- the amount of type I collagenase (e.g., MMP-1) produced was quantified using a method according to the ELISA kit (AP biotech RPN2610).
- Table 4 and FIG. 1 show the effect of the extract of the soybean of the present disclosure on the amounts of MMP-1 and procollagen in the artificial skin irradiated with ultraviolet rays.
- 8-week-old nude mice (OrientBio, SKH-1 species, and average weight of 26.2 g) were divided into two groups, each having seven mice.
- one group is a control group
- the other group is a test group.
- 2.5 mg/10 ml and 5.0 mg/10 ml of an extract solution were each administered to a mouse to be 25 mg/kg/day and 50 mg/kg/day, respectively, wherein the extract solution was obtained by dissolving the lyophilized extract of Section 1 in an aqueous solution containing 2 % ethanol, 1 % Tween 80, and 0.8 % carboxymethyl cellulose (CMC).
- CMC carboxymethyl cellulose
- an aqueous solution containing 2 % ethanol, 1 % Tween 80, and 0.8 % CMC was administered to a mouse.
- the administration was performed by direct administration to the stomach of the mouse using an oral zoned.
- the soybean extract was able to be administered to the mouse at doses of 25 mg/kg/day and 50 mg/kg/day. After this administration, ultraviolet rays were irradiated to the mouse.
- MED minimal erythema dose
- 2 MEDs of ultraviolet ray was irradiated three times a week for two weeks
- 3 MEDs of ultraviolet ray was irradiated twice a week for two weeks
- 4 MEDs of ultraviolet ray was irradiated twice a week for two weeks. That is, the irradiation was performed for a total of eight weeks.
- the mean form factor is calculated from the following formula with the most accurate and reliable measurement method.
- the mean form factor is represented by analyzing the depth and length of the shadows of the wrinkles created by irradiating the skin replica with the light source.
- FIG. 2 is a diagram showing the effect of the extract of the soybean of the present disclosure on the wrinkle pattern and the winkle-related mean form factor in the artificial skin irradiated with ultraviolet rays. Referring to FIG .2, it was confirmed that the group to which the soybean cultivar SCEL-1 was orally administered at a dose of 50 mg/kg/day had the mean form factor value of 0.7, indicating that the wrinkle-improving effect was excellent compared to other test groups treated with other soybeans.
- FIG. 3 shows the results of the H&E staining performed on the mouse skin irradiated with ultraviolet rays and effects of the extract of the soybean of the present disclosure the collagen density in the mouse skin.
- a test group treated with the soybean SCEL-1 showed an effect of preventing the skin from thickening by referring to that the epidermal thickness of the skin was significantly small upon ultraviolet irradiation.
- the soybean extract was subjected to mass spectrometry, so as to analyze active ingredients in the soybean extract.
- the analysis of components in the soybean extract was carried out by using an Agilent 1260 HPLC system and a Bruker MicrOTOF-Q II mass spectrometer.
- a Prevail C18 column 250 mm x 4.6 mm, 5 um
- Solvent A of a mobile phase consisting of 95% water/5% acetonitrile (0.1% formic acid)
- Solvent B of a mobile phase consisting of 95% acetonitrile/5% water (0.1% formic acid
- the flow rate of the solvent was set at 0.7 ml/min, and the concentration gradient conditions of the solvent used for the separation of components are shown in Table 7.
- the temperature of the column was maintained at 35 °C, and 10 ⁇ L of the sample was injected into the column.
- a mass spectrometer was used to analyze ingredients contained in the soybean extract under conditions of mode: ESI(+); mass range: 50 m/z to 800 m/z; nebulizing gas: 8 L/min; source gas temperature: 180 °C; capillary voltage: +4,500 V; and cone voltage: 35 V.
- the amount of each of epicatechin and procyanidin B2 contained in the selected cultivar named SCEL-1 was in a range of about 0.32% to about 0.46% and in a range of about 1.0% to about 2.0%, respectively, which was significantly higher than the amounts thereof in Wonheug, which were respectively in a range of about 0.14% to about 0.15 % and in a range of about 0.33% to about 0.35%.
- SCEL-1 selected on the basis of cyanidin-3-O-glucoside which is a representative substance of anthocyanin antioxidants, cyanidin-3-O-glucoside, procyanidin B2, and epicatechin were contained at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48. Meanwhile, in Wonheug, cyanidin-3-O-glucoside, procyanidin B2, and epicatechin were contained at a ratio of 1: 0.43 to 0.70 : 0.19 to 0.25.
- a composition for skin health may be used to prevent or improve skin wrinkles and/or skin inflammation.
- a method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, or preventing occurrence of skin inflammation in a subject or improving skin inflammation in a subject may be used to prevent occurrence of skin wrinkles in a subject or improve skin wrinkles in a subject, or to prevent skin inflammation in a subject or improve skin inflammation in a subject.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Dermatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
Provided are a seed of new soybean cultivar SCEL-1, a plant grown therefrom and parts of the plant, and an extract extracted from the seed.
Description
CROSS-REFERENCE TO RELATED APPLICATION
This application claims the benefit of Korean Patent Application No. 10-2018-0078246, filed on July 5, 2018, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein in its entirety by reference.
Field
One or more embodiments relate to a composition for skin health, the composition including an extract of a seed of new soybean cultivar SCEL-1
Soybeans are an abundant crop source of vegetable proteins, and contain not only proteins, but also a variety of excellent functional materials including unsaturated fatty acids, amino acids, isoflavones, and phenolic acids. In this regard, soybeans are being used as a protein source to replace animal proteins.
Researchers, CHA, Baecheon et al. (The Korean Society of Pharmacognosy, Vol. 27(3): pages 190-195 (published in 1996)), discovered that an extract obtained by performing an extraction process (hereinafter referred to as an ethanol extract) on a wild-type soybean Glycine soja Siebold et Zucc. by using ethanol contains (-)-epicatechin, unlike an ethanol extract of another soybean soybean Glycine
max (L.) Merrill. In addition, the researchers also discovered that the ethanol extract of the wild-type soybean has antioxidative activity. However, it is not disclosed that the soybean Glycine max (L.) Merrill contains (-)-epicatechin.
Therefore, there is a demand for a new soybean cultivar having excellent antioxidant and/or other physiological functions, compared to the known soybeans, or for a composition including an extract obtained from the new soybean cultivar.
One or more embodiments include a composition for skin health, the composition including, as an active ingredient, an extract obtained by performing an extraction process on a seed of soybean Glycine
max (L.) Merrill cultivar SCEL-1 by using water, C1-C6 alcohol, or a mixture thereof, wherein the seed contains cyanidin-3-O-glucoside, procyanidin B2, and epicatechin, wherein an amount of the procyanidin B2 is greater than that of the cyanidin-3-O-glucoside, and a representative sample of the seed is deposited with the Korean Agricultural Culture Collection(KACC), which is an International Depository Authority(IDA) under the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure under the accession number KACC 88002BP on November 8, 2018.
One or more embodiments include a composition for skin health, the composition including, as an active ingredient, cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof, at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48 on a weight basis.
One or more embodiments include a method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, or preventing occurrence of skin inflammation in a subject or improving skin inflammation in a subject, the method including administrating the composition to a subject.
Additional aspects will be set forth in part in the description which follows and, in part, will be apparent from the description, or may be learned by practice of the presented embodiments of the disclosure.
A first aspect of the present disclosure provides a composition for skin health, the composition including, as an active ingredient, an extract obtained by performing an extraction process on a seed of soybean Glycine max (L.) Merrill cultivar SCEL-1 by using water, C1-C6 alcohol, or a mixture thereof, wherein the seed contains cyanidin-3-O-glucoside, procyanidin B2, and epicatechin, wherein an amount of the procyanidin B2 is greater than that of the cyanidin-3-O-glucoside, and a representative sample of the seed is deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018.
In the seed of the present disclosure, cyanidin-3-O-glucoside and procyanidin B2 may be contained at a ratio of 1: 0.8 or more, 1: 0.8 to 5.0, 1: 0.8 to 3.0, 1: 0.8 to 2.5, or 1: 0.8 to 2.1, on a weight basis. For example, in the seed of the present disclosure, cyanidin-3-O-glucoside and procyanidin B2 may be contained at a ratio of 1: 2.0 or more, 1: 2.0 to 5.0, 1: 2.0 to 3.0, 1: 2.0 to 2.5, or 1: 2.0 to 2.1, on a weight basis. The term "amount" as used herein may be calculated based on an extract obtained by pulverizing the seed and incubating the pulverized seed at a temperature in a range of about 25 ℃ to about 55 ℃ for 6 hours by using 70 (v/v)% aqueous ethanol. The term "amount" as used herein is described by way of example, and should be understood as being variable depending on soil, climate conditions, and an individual seed that are considered for production of a plant body of a seed.
In the seed of the present disclosure, a total amount of procyanidin B2 and epicatechin may be, on a weight basis, twice or more or 2.5 times or more, for example, 2.6 times to 5.1 times greater than that of a cultivar named Wonheug, which is a standard cultivar.
In the composition of the present disclosure, a total amount of cyanidine-3-O-glucoside, procyanidine B2, and epicatechin in the extract may be in a range of about 2.1 % to about 3.4 % based on a total weight of the extract, and a total amount of procyanidine B2 and epicatechin in the extract may be in a range of about 1.31 % to about 2.4 % based on a total weight of the extract.
In the composition of the present disclosure, cyanidin-3-O-glucoside, procyanidin B2, and epicatechin may be contained at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48 on a weight basis.
In the composition of the present disclosure, an amount of the extract may be in a range of about 0.001 % to about 99.9 %, about 0.005 % to about 99.9 %, about 0.01 % to about 80.0 %, about 0.01 % to about 60.0 %, about 0.01 % to about 50.0 %, about 0.01 % to about 30.0 %, about 0.01 % to about 20.0 %, about 0.01 % to about 15.0 %, about 0.01 % to about 10.0 %, about 0.01 % to about 5.0 %, about 0.1% to about 99.9 %, about 1.0 % to about 80.0 %, about 5.0 % to about 60.0 %, about 5.0 % to about 50.0 %, about 5.0 % to about 30.0 %, about 30.0% to about 50.0 %, about 40.0% to about 80.0 %, about 15.0 % to about 70.0 %, or about 50.0% to about 90.0 %, based on a total weight of the composition.
When the seed of the present disclosure germinates and forms a plant body, such a plant body may have characteristics listed in Table 1. Unless otherwise described in the specification, the characteristics of the soybean cultivar may be measured and verified according to "The guidelines for investigation of characteristics of each crop for examination of new varieties: Soybean Glycine
max (L.) Merrill (Korea Seed Variety Service (KSVS) of the Ministry of Agriculture, Food and Rural Affairs (MAFRA), 2014: http.//www.seed.go.kr)", wherein the guidelines determine matters necessary to explain the characteristics of the varieties for each crop in Annex 1 in Article 2 of the Seed Management Guidelines pursuant to Article 30 of the Act of Protection of New Varieties of Plants and Article 33 of the Enforcement Decree of the same Act, and the directions for the qualification tests necessary for cultivation examination according to Article 47 of Enforcement Regulation of the same Act. In addition, unless otherwise described herein, quantitative traits among the characteristics of the soybean cultivar are represented as mean values.
The composition may not include any extract or active ingredient other than the extract described above, and in this regard, the extract described above may be included as the only extract or active ingredient.
A second aspect of the present disclosure provides a composition for skin health, the composition including, as an active ingredient, cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48 on a weight basis.
In the composition of the present disclosure, an amount of each of cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof may be in a range of about 0.001% to about 99.9 %, about 0.005% to about 99.9 %, about 0.01% to about 80.0 %, about 0.01% to about 60.0 %, about 0.01% to about 50.0 %, about 0.01% to about 30.0 %, about 0.01 % to about 20.0 %, about 0.01 % to about 15.0 %, about 0.01 % to about 10.0 %, about 0.01 % to about 5.0 %, about 0.1% to about 99.9 %, about 1.0 % to about 80.0 %, about 5.0 % to about 60.0 %, about 5.0 % to about 50.0 %, about 5.0 % to about 30.0 %, about 30.0% to about 50.0 %, about 40.0% to about 80.0 %, about 15.0% to about 70.0 %, or about 50.0% to about 90.0 %.
The composition of each of the first aspect and the second aspect of the present disclosure may be food, a cosmetic composition, or a pharmaceutical composition.
In the second aspect, the expression "physiologically acceptable salt thereof" as used herein includes a meaning of "pharmaceutically acceptable salt thereof". The term expression "pharmaceutically acceptable salt thereof" refers to possible use on animals, more particularly, to humans, without having significant toxic effects when used in a typical medicinal dosage. This expression infers that, for example, one that can be or is approved by a government or regulatory organization equivalent thereto, or one that is listed in the pharmacopoeia or recognized in other general pharmacopoeias.
In the second aspect, the expression "pharmaceutically acceptable salt thereof" refers to a salt according to an embodiment of the present disclosure being pharmaceutically acceptable and having pharmacological activity of a parent compound. Such a salt may include: (1) an acid addition salt formed of an inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, or the like; or an organic acid, such as acetic acid, propionic acid, hexanoic acid, cyclopentane propionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, 3-(4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, 4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid, 4-toluenesulfonic acid, camphorsulfonic acid, 4-methylbicyclo[2,2,2]-oct-2-en-1-carboxylic acid, glucoheptonic acid, 3-phenylpropionic acid, trimethylacetic acid, tert-butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, or the like; or (2) a salt formed by substitution of an acidic proton in a parent compound.
In the first aspect and the second aspect, the term "active ingredient" refers to an ingredient which exhibits a desired activity alone or an ingredient capable of exhibiting activity with a carrier that is not active by itself.
In the first aspect and the second aspect of the present disclosure, the expression "for skin health" refers to preventing occurrence of skin wrinkles or improving skin wrinkles, or preventing skin inflammation or improving skin inflammation, wherein a cause of the skin wrinkles or the skin inflammation may include external stress, such as ultraviolet rays or oxidizing agents on the skin; or internal stress.
In the composition for skin health according to an aspect of the present disclosure, the composition may be also able to prevent or treat skin wrinkles and/or skin inflammation.
In the second aspect of the present disclosure, the composition may include, as an active ingredient, an extract obtained by performing an extraction process on a soybean cultivar seed by using water, C1-C6 alcohol, or a mixture thereof, wherein a representative sample of the soybean cultivar seed is deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018.
In one embodiment, the composition may be one that is effective for skin health, and more particularly, may be one that is effective for skin damage caused by ultraviolet rays or oxidative stress.
When the composition of the present disclosure is applied to medicines, the composition may be formulated into a dosage form in a solid, semi-solid, or liquid form for oral or parenteral administration by adding a commonly used inorganic or organic carrier thereto which includes the composition as an active ingredient.
Examples of the dosage form for oral administration may include tablets, pills, granules, capsules, powders, infinitesimal grains, emulsions, syrup, pellets, and the like. In addition, examples of the dosage form for parenteral administration may include injections, instillations, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. To formulate the active ingredient of the present disclosure, the active ingredient may be easily formulated according to the conventional method, and a surfactant, an excipient, a coloring agent, a flavoring agent, a preservative, a stabilizer, a buffer, a suspension, and other common adjuvants may be used as appropriate.
The composition according to the present disclosure may be administered orally, parenterally, rectally, topically, transdermally, intravascularly, intramuscularly, intraperitoneally, subcutaneously, and the like.
In addition, a dosage of the active ingredient may vary depending on the age, gender, and weight of a subject to be treated, a particular disease or pathological condition to be treated, the severity of a disease or pathological condition, a route of administration, and a determination made by a prescriber. The determination of the dosage based on these factors may be within a level of one of ordinary skill in the art, and the dosage may be in a range of about 0.001 mg/day to about 2,000 mg/kg/day, more particularly, a range about 0.5 mg/kg/day to about 1,500 mg/kg/day.
Regarding the composition for skin health according to an aspect of the present disclosure, the composition may include a health food composition.
In one embodiment, the composition may be processed into a drink, fermented milk, a cheese, a yogurt, a juice, a probiotic agent, and a health supplement, each including the composition. In addition, the composition may be used in a variety of other food additives.
In one or more embodiments, the composition may contain other components that exhibit a synergistic effect with main effects within a range that does not damage the intended main effects of the present disclosure. For example, to improve physical properties, the composition may further include an additive, such as a flavoring agent, a dye, a bactericide, an antioxidant, a preservative, a moisturizer, an instillation, an inorganic salt, an emulsifier, or a synthetic polymer. In addition, the composition may further include an adjuvant component, such as a water-soluble vitamin, an oil-soluble vitamin, a polymeric peptide, a polymeric polysaccharide, or a seaweed extract. The components above may be appropriately selected and mixed by one of ordinary skill in the art without difficulty depending on the formulation and purpose of use, and an amount of the components to be added may be selected within a range that does not damage the objects and effects of the present disclosure.
The composition of the present disclosure may be in various forms, such as a solution, an emulsion, a viscous mixture, a tablet, a powder, and the like, and may be administered by various methods using a simple drink, an injection, a spray, or a squeezer.
The composition may include a cosmetic composition, and may be formulated in a parenteral dosage form. An example of the parenteral dosage form may include an injection or an external skin application, and examples of the external skin application may include a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or any combination thereof.
In the external skin application, components such as typical cosmetics or medicines used for external skin applications, for example, an aqueous component, an oily component, a powder component, an alcohol component, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, an antiseptic, an antioxidant, a surfactant, a flavoring agent, a dye, various skin nutrients, or any combination thereof, may be appropriately mixed as needed.
In the external skin application, a chelating agent such as disodium edentate, trisodium edentate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, and the like; caffeine, tannin, verapamil, licorice extract, glabridin, hot water extract of calines from fruit, and various herb medicines; medicines such as tocopherol acetate, glycyrrhizic acid, tranexamic acid, or a derivative or salt of any of the foregoing; vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, and kojic acid; and sugars such as glucose, fructose, and trehalose, may be mixed as appropriate.
In the composition for skin health according to an aspect of the present disclosure, the extract of the soybean cultivar SCEL-1 may be obtained by performing an extraction process on the soybean cultivar SCEL-1 by using water, C1-C6 alcohol, or a mixture thereof. In detail, the extract of the soybean SCEL-1 may be obtained by using methanol, ethanol, propanol, butanol, pentanol, or hexanol.
The extract of the soybean cultivar SCEL-1 of the present disclosure may include a crude extract obtained by performing an extraction process thereon by using a solvent, such as water, C1-C6 alcohol, or a mixture thereof. The C1-C6 alcohol may be, for example, methanol or ethanol. When an extraction process is performed on a soybean by using a solvent, a volume of the solvent may be about 2 times to about 15 times, about 3 times to about 15 times, about 5 times to about 15 times, or about 10 times greater than the soybean. The extraction process may include heat extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction, and there is no limitation as long as the extraction process is obvious to one of ordinary skill in the art. The extraction process may be performed at room temperature, but for better efficient extraction, the extraction process may be performed under warm conditions. For example, the extraction process may be performed at a temperature in a range of about 40 ℃ to about 100 ℃, about 40 ℃ to about 80 ℃, about 40 ℃ to about 60 ℃, or at a temperature of about 50 ℃. The time for which the extraction process is performed may be in a range of about 2 hours to about 8 hours, about 4 hours to about 8 hours, about 5 hours to about 7 hours, about 5.5 hours to about 6.5 hours, or may be about 6 hours. The temperature may vary depending on conditions including a solvent used for the extraction process. To obtain a greater amount of active ingredients, the extraction process may be performed one or more times. For example, an extract obtained by combining all the extracts from the extraction process performed one to 5 times, one to 4 times, or 3 times consecutively may be used.
The extract of the soybean cultivar SCEL-1 of the present disclosure may include a crude extract of the soybean cultivar SCEL-1, and may be contained as a water-soluble fraction of the organic solvent obtained by further extraction performed on the crude extract.
Examples of the organic solvent may include hexane, methylene chloride, ethyl acetate, n-butanol, and the like. According to the method of the present disclosure, the extract or the water-soluble fraction thereof may be used as it is. In one embodiment, the extract may be used as a concentrate obtained by concentration, and in one or more embodiments, the extract may be used in a lyophilized form obtained by concentration followed by lyophilization.
A third aspect of the present disclosure provides a method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, or preventing skin inflammation in a subject or improving skin inflammation in a subject, the method including administrating the composition of the first or second aspect to a subject.
The administrating may be oral or parenteral administration. The method according to an aspect of the present disclosure may be able to prevent occurrence of skin wrinkles in a subject or to improve skin wrinkles in a subject. The method according to an aspect of the present disclosure may be also to prevent occurrence of skin inflammation in a subject or to improve skin inflammation in a subject.
The method of the present disclosure may include administrating the composition to the skin of a subject. Here, the administrating may include coating or applying the composition to the skin. The method of the present disclosure may be considered as a make-up method, and may prevent or reduce the occurrence of reactive oxygen species (ROS) in the skin. The ROS may be produced when ultraviolet rays are irradiated to the skin.
The subject may include a mammal including a vertebrate, or fish. The vertebrate may include a cow, a pig, a cat, a dog, or a sheep. That is, the subject may be a mammal other than a human.
The above and other aspects, features, and advantages of certain embodiments of the disclosure will be more apparent from the following description taken in conjunction with the accompanying drawings, in which:
FIG. 1 is a diagram showing effects of an extract of a selected from soybean cultivar on the improvement of wrinkles of the skin damaged by ultraviolet rays in a nude mouse;
FIG. 2 shows the results of staining a tail tissue derived from a nude mouse irradiated with ultraviolet rays; and
FIG. 3 shows the results of the H&E staining performed on the mouse skin irradiated with ultraviolet rays and effects of an extract of a soybean on the collagen density in the mouse skin.
Reference will now be made in detail to embodiments, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout. In this regard, the present embodiments may have different forms and should not be construed as being limited to the descriptions set forth herein. Accordingly, the embodiments are merely described below, by referring to the figures, to explain aspects of the present description. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items. Expressions such as "at least one of," when preceding a list of elements, modify the entire list of elements and do not modify the individual elements of the list.
Examples 1: Breeding and characteristics of new
cultivar
1.
Breeding of
cultivar
20 soybean seed grains, which are black flat-shaped granules collected as genetic resources from Idong-eup, Yongin-si, Gyeonggi-do, Korea, were prepared. As a result of cultivating the soybean seeds, separation occurred in terms of shape and color of the flowers, and in this regard, it was confirmed that the traits of the soybean seeds were not fixed. For example, the flower color appeared separately as white or pink. In this regard, the traits of the soybean seeds did not seem to be fixed, and thus, the soybean seeds were not recognized as species due to lack of stability and uniformity.
Therefore, the inventors of the present disclosure bred a cultivar by using the soybean seeds according to a pure line selection breeding method. The pure line selection was carried out from 2013 to 2017, and more particularly, it was carried out by the whole crop experimental field in the Rural Development Administration (RDA): National Institute of Crop Science (NICS) located in Suwon, Republic of Korea from 2013 to 2014, and then, in the field located in Iseo-myeon, Wanju-gun, Republic of Korea from 2015 to 2017.
A specific process of the pure line selection from the collected genetic resources of the soybean is as follows: as genetic resources, 20 soybean seeds were sown at the beginning of June every year from 2013 to 2016 in the experimental field affiliated with the RDA; then, 1 seed was selected for the purity improvement at the end of November and then harvested as a sowing seed for the following year. After four years of the pure line selection process, one finally fixed pure line seed was selected.
To confirm uniformity, traits, such as hypocotyl color, flower color, trichome color, legume color, leaf shape, growth type, flowering periods, and maturity, were examined every year. In the pure line separation process performed on the collected genetic resources, separation was observed in the hypocotyls color and the flower color in 2013, whereas no separation was observed with respect to the other traits. In addition, the progeny selected in 2013 was found to maintain uniformity in target traits among traits examined from 2014 to 2017. The distinguishable features between a standard cultivar (named Wonheug) and a control cultivar (named Cheongja-3) are clearly shown in Table 1 in terms of growth type, days to flowering, hypocotyl color, weight of 100 beans, and leaf shape. Table 1 shows the characteristics of the finally selected pure line. In Table 1, the finally selected line (cultivar) was named SCEL-1.
Source | Growth type (determinate, indeterminate) | Number of days to flowering | Flower color (violet or white) | Hypocotyl color (violet or green) | Trichome color(brown, light gray) | Testa | Hilum color | 100-seed weight(g) | Leaf shape |
Wonheug (standard) | Determinate | 66 | Violet | Violet | Brown | Black | Black | 11.6 | Globular |
Cheongja-3 (control) | Determinate | 70 | Violet | Violet | Brown | Black | Black | 40.7 | Globular |
Selected cultivar (SCEL-1) | Indeterminate | 58 | White | Green | Brown | Black | Black | 8.8 | Oblique ellipse |
2.
Identification of
cultivar
characteristics relative to control cultivar
To compare the agricultural performance of the finally selected pure line cultivar SCEL-1 relative to the existing cultivars, the performance of each cultivar was identified by repeating the randomized block design three times by using the Wonheug bean as a standard cultivar and Cheongja-3 as a control cultivar in the field affiliated with the NISC in summer of 2017. Here, the agronomic traits of each cultivar were examined by cultivating seeds after sowing two seed grains per row in an experimental plot (60 cm X 15 cm) containing four rows (4 meters long) on June 8, 2017, and then cultivating the seeds. The Wonheug bean (Cultivar Application Publication Number: 2010-341) is a microcarpa bean cultivar with a black seed coat developed by the NICS in 2009, and is currently the most cultivated cultivar in farming. The Wonheug bean is similar to the selected cultivar SCEL-1, and thus, may be used as a standard cultivar for the comparison. The control cultivar, Cheongja-3 (Cultivar Application Publication Number: 2005-176), was developed by the NICS in 2004 and is a soybean cultivar that has a black seed coat, is large-sized, and is used for cooking with rice. The Wonheug bean and Cheongja-3 are commercially available from the Korean Seed and Variety Service (KSVS) or the like.
The agronomic traits and traits examined for the performance testing were examined mainly in terms of yield component traits, such as stem length, number of nodes, number of branches, and the like. To examine such yield component traits, 10 plants with repeated nodes were examined during a maturation period in terms of stem length, number of nodes, number of branches, number of pods, 100-seed weight, yield per plant, yield per area, and yield per 10 acres (a).
Tables 2 and 3 show the characteristics of the selected soybean cultivar, the standard cultivar, and the control cultivar.
Source | Length of stem (cm) | Number of nodes | Number of branches | Seed number per |
||
1 pod | 2 pods | 3 pods | ||||
Wonheug (standard) | 56.6±4.3 | 14.7±1.7 | 6.7±2.1 | 18.8±2.3 | 201±21 | 32±15.2 |
Cheongja-3 (control) | 71.9±2.7 | 14.1±0.3 | 7.3±0.6 | 13.8±4.9 | 81±12 | 4.7±0.8 |
Selected cultivar (SCEL-1) | 82.9±1.4 | 15.8±0.5 | 6.4±0.3 | 12.9±2.3 | 133±44 | 38.3±8.6 |
Source | Total number of pods | Number of grains | 100-seed weight (g) | Yield per plant(g) | Yield per area(kg) | Yield per 10 acre (a)(kg) |
Wonheug (standard) | 252±31 | 512±70 | 11.6±0.2 | 32.9±3.3 | 6.6±0.7 | 587±60 |
Cheongja-3 (control) | 100±12 | 191±25 | 40.7±3.1 | 26.7±3.2 | 5.3±0.6 | 477±57 |
Selected cultivar (SCEL-1) | 184±54 | 397±113 | 9.4±0.4 | 23.1±2.0 | 4.6±0.4 | 413±36 |
Referring to Tables 2 and 3, it was found from the examination that the selected cultivar was longer than the standard cultivar, Wonheug, by 26 cm, and had one more node than the standard cultivar, Wonheug. In addition, regarding the number of branches, it was found from the examination that the selected cultivar was had about 6.4 branches similar to the standard cultivar, and regarding the number of pods, the constitution ratio of the 1-pod, 2-pods, and 3-pods was similar to the standard cultivar. Regarding the total number of pods, the number of grains, the yield per plant, and the yield per area, which indicate the yield component traits, the selected cultivar had 184 pods, 397 grains, 23.1 g of the yield per plant, and 4.6 g of the yield per area, wherein the resulting numbers and yields were lower than those of the standard cultivar. Here, 100 grains were weighed at 9.4 g, and in this regard, the selected cultivar had small grains as compared with the standard cultivar. When the yield per 10a was calculated based on the results above, the selected cultivar showed 413 kg/10 a, which was only about 70% of the yield per 10a of the standard cultivar, Wonheug. However, the amounts of procyanidin B2 and epicatechin, which are functional substances, were about 301% and 217%, respectively. In detail, the amount of procyanidin B2 was 149.4 ug/100 mg, 49.5 ug/100 mg, and 34.3 ug/100 mg in the selected cultivar SCEL-1, Wonheug, and Cheongja-3, respectively. The amount of epicatechin was 46.9 ug/100 mg, 21.6 ug/100 mg, and 20.1 ug/100 mg in the selected cultivar SCEL-1, Wonheug, and Cheongja-3, respectively. Here, the amounts of procyanidin B2 and epicatechin were confirmed by an extraction process performed according to the method of Section 1 in Example 2.[0016] According to the experiments, the cultivar SCEL-1 was found to have high uniformity and was distinguished from the standard cultivar and the existing cultivars by the comparison, and was also able to be recognized as an independent cultivar with distinct characteristics in terms of the agronomic traits, the yield ability, and the functional material contents.
This newly selected cultivar of a black small-sized bean having an oblique ellipse shape was named SCEL-1, and was deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018. FIG. 1 shows a schematic breeding diagram of a soybean cultivar according to the present disclosure.
Example 2: Extract obtained by performing an extraction process on a newly selected soybean seed by using water, alcohol, or a mixture, and use of the extract
In this Example, an extract was prepared from a seed of the newly selected soybean cultivar SCEL-1 of Example 1, and an effect of the extract was identified.
1.
Preparation of an extract
Immediately before analyzing a seed of the selected cultivar, the seed was pulverized by using a high-speed pulverizer (Wonder Blender, 820W, 30000 RPM, Sanplatec Corp) to prepare powdered seed. 1 g of the powdered seed thus obtained was mixed with 100 ml of 70 (v/v)% aqueous ethanol in a glass tube, and the mixed solution was stirred at a temperature of 50 ℃ for 6 hours by using a magnetic bar. Then, the resulting extract was filtered through filter paper (Garde No.131 Qualitative filter paper, Advantec).
Afterwards, the ethanol layer was dried in a nitrogen gas drier (hurricane-Eagle, Chongmin Technology) for 1 hour, and then, was lyophilized for one day to completely remove moisture,thereby obtaining 0.19 g (standard deviation SD: ±0.015) of a soybean extract. In the experiments, for use of the soybean extract, it was dissolved at a concentration of 30 mg/ml in 50% v/v ethanol, and was subjected to the HPLC analysis.
2.
Confirmation of wrinkle improving effect
By using the extract obtained in Section 1, the efficacy of the extract of improving wrinkles damaged by ultraviolet rays was confirmed through an experiment using the EpiDermFTTM 3D skin product and a nude mouse model.
(1) Confirmation of using artificial skin
In detail, the EpiDermFTTM 3D skin product manufactured by MatTek Company was purchased and used in this experiment. After a 6-well plate was filled with 900 ul of the medium provided by the manufacturer, the prepared 3D skin product was placed on each well of the plate, and then, cultured in an incubator under the conditions of 37 ℃, 5 % CO2, and 100 % humidity. After 24 hours, ultraviolet B (UVB) rays (40 mJ/cm2) were irradiated, and the medium in each cell was replaced with a medium containing test materials in an amount of 10 ug/ml, 30 ug/ml, and 100 ug/ml, respectively, After 72 hours of incubation, the culture medium at the bottom, i.e., the culture medium containing collagenase (e.g., MMP-1) and procollagen that were secreted from the artificial skin product was harvested. Then, the amount of type I collagenase (e.g., MMP-1) produced was quantified using a method according to the ELISA kit (AP biotech RPN2610). In addition, the same supernatant was used to quantify the amount of residual, undegraded procollagen using a method according to the ELISA kit (Takara MK101). Each quantified value was expressed as the amount of protein per ml, And the results of the quantification are shown in Table 4 and FIG. 1.
Table 4 and FIG. 1 show the effect of the extract of the soybean of the present disclosure on the amounts of MMP-1 and procollagen in the artificial skin irradiated with ultraviolet rays.
Sample | Concentration (ug/ml) | MMP-1 biosynthesis (pg/ml) | Amount of residual procollagen |
Untreated group | 3980 ± 250 | 19000 ± 320 | |
UVB-induced group(40 mJ/cm2) | 11800 ± 300 | 15000 ± 220 | |
Soybean SCEL-1 | 10 | 9980 ± 220 | 17000 ± 130 |
30 | 8100 ± 230 | 19000 ± 260 | |
100 | 7600 ± 180 | 22000 ± 379 | |
Black soybbean | 10 | 11200 ± 350 | 16000 ± 400 |
30 | 10400 ± 320 | 17300 ± 480 | |
100 | 8500 ± 350 | 18500 ± 500 | |
Rhynchosia nulubilis | 10 | 10800 ± 340 | 16800 ± 496 |
30 | 10100 ± 380 | 18100 ± 550 | |
100 | 8300 ± 220 | 19200 ± 460 |
Referring to Table 4 and FIG. 1, it was confirmed that, by treating the extract of the soybean cultivar SCEL-1, the biosynthesis of matrix metalloprotease (MMP-1), which is type I collagenase caused by UVB, was reduced. Accordingly, it was also confirmed that the degradation of procollagen was prevented. As a result of comparing three beans, it was confirmed that soybean cultivar SCEL-1 showed significantly excellent effects compared to other types of black beans.
(2) Confirmation of using an animal model
When orally administering the extract obtained in Section 1, the efficacy of the extract of improving skin wrinkles was confirmed through an experiment using a nude mouse model.
8-week-old nude mice (OrientBio, SKH-1 species, and average weight of 26.2 g) were divided into two groups, each having seven mice. Here, one group is a control group, and the other group is a test group. In the test group, based on a weight, 2.5 mg/10 ml and 5.0 mg/10 ml of an extract solution were each administered to a mouse to be 25 mg/kg/day and 50 mg/kg/day, respectively, wherein the extract solution was obtained by dissolving the lyophilized extract of Section 1 in an aqueous solution containing 2 % ethanol, 1 % Tween 80, and 0.8 % carboxymethyl cellulose (CMC). In the control group, based on a volume, an aqueous solution containing 2 % ethanol, 1 % Tween 80, and 0.8 % CMC was administered to a mouse. Here, the administration was performed by direct administration to the stomach of the mouse using an oral zoned. In this regard, the soybean extract was able to be administered to the mouse at doses of 25 mg/kg/day and 50 mg/kg/day. After this administration, ultraviolet rays were irradiated to the mouse.
Along with the administration of the extract solution or 2 % ethanol, 1% Tween 80, and 0.8 % CMC aqueous solution, to individual mice in each of the control group and the test group, 1 minimal erythema dose (MED) of ultraviolet ray was irradiated three times a week for two weeks, 2 MEDs of ultraviolet ray was irradiated three times a week for two weeks, 3 MEDs of ultraviolet ray was irradiated twice a week for two weeks, and 4 MEDs of ultraviolet ray was irradiated twice a week for two weeks. That is, the irradiation was performed for a total of eight weeks. After oral administration of each sample once a day for 8 weeks, a skin replica was manufactured using silicone (SILFLO impression material, Flexico, Tokyo, Japan). Then, the state of wrinkles was measured using a fine wrinkle measuring meter (Visioline VL650, Courage + Khazaka, Koln, Germany) to observe the wrinkle pattern, and the wrinkle-related factor values were identified.
Among the wrinkle-related factor values, the mean form factor is calculated from the following formula with the most accurate and reliable measurement method. The mean form factor is represented by analyzing the depth and length of the shadows of the wrinkles created by irradiating the skin replica with the light source.
Mean form factor = 1-(Wrinkle depth/wrinkle length). Here, this value is always between 0 and 1, and the closer the measured value is to 1, the better the wrinkle improving effect is meant. The results are shown in FIG.2. FIG. 2 is a diagram showing the effect of the extract of the soybean of the present disclosure on the wrinkle pattern and the winkle-related mean form factor in the artificial skin irradiated with ultraviolet rays. Referring to FIG .2, it was confirmed that the group to which the soybean cultivar SCEL-1 was orally administered at a dose of 50 mg/kg/day had the mean form factor value of 0.7, indicating that the wrinkle-improving effect was excellent compared to other test groups treated with other soybeans.
In addition, the skin tissue of the nude mouse after the end of the experiment was treated with formalin, and to observe the thickness of the skin tissue, the skin tissue was subjected to the H&E staining. The results are shown in Table 5. Also, to observe changes in collagen, Masson Trichome staining was performed on the skin tissue, and the results are shown in Table 6 and FIG. 3. FIG. 3 shows the results of the H&E staining performed on the mouse skin irradiated with ultraviolet rays and effects of the extract of the soybean of the present disclosure the collagen density in the mouse skin.
Sample | Oral dose (mg/kg/day) | Epidermal thickness |
Untreated group | 15.5 ± 0.84 | |
UVB (40 mJ/cm2) | 55.2 ± 0.08 | |
Soybean SCEL-1 | 25 | 46.5 ± 3.35 |
50 | 43.9 ± 1.19 | |
General |
25 | 55.6 ± 0.98 |
50 | 55.7 ± 1.39 | |
Rhynchosia nulubilis(Wonju) | 25 | 51.9 ± 2.17 |
50 | 55.0 ± 0.38 |
Referring to Table 5, a test group treated with the soybean SCEL-1 showed an effect of preventing the skin from thickening by referring to that the epidermal thickness of the skin was significantly small upon ultraviolet irradiation.
Sample | Oral dose (mg/kg/day) | Amount of collagen (%) |
Untreated group | 193.8 ± 5.6 | |
UVB (40 mJ/cm2) | 100 ± 7.1 | |
Soybean SCEL-1 | 25 | 147.6 ± 11.5 |
50 | 176.1 ± 6.8 | |
|
25 | 150.7 ± 5.1 |
50 | 161.0 ± 7.2 | |
|
25 | 147.3 ± 4.7 |
50 | 159.2 ± 7.2 |
Referring to FIG. 3 and Table 6, a test group treated with the soybean SCEL-1 was superior to other types of beans in preventing the collagen breakage during ultraviolet irradiation and restoring the collagen activity.[0041] From the results above, it was confirmed that the soybean SCEL-1 extract showed excellent efficacy for the improvement of skin tissue or wrinkles damaged by ultraviolet rays.
3.
Analysis of components of soybean extract
The soybean extract was subjected to mass spectrometry, so as to analyze active ingredients in the soybean extract.
In detail, the analysis of components in the soybean extract was carried out by using an Agilent 1260 HPLC system and a Bruker MicrOTOF-Q II mass spectrometer. Here, for the column analysis, a Prevail C18 column (250 mm x 4.6 mm, 5 um), Solvent A of a mobile phase consisting of 95% water/5% acetonitrile (0.1% formic acid), and Solvent B of a mobile phase consisting of 95% acetonitrile/5% water (0.1% formic acid) were used. Here, the flow rate of the solvent was set at 0.7 ml/min, and the concentration gradient conditions of the solvent used for the separation of components are shown in Table 7.
Time (min) | A(%) | B(%) | Flow rate (ml/min) | Maximum pressure (bar) |
0.00 | 95.00 | 5.00 | 0.700 | 1000.00 |
3.00 | 95.00 | 5.00 | 0.700 | 1000.00 |
23.00 | 50.00 | 50.00 | 0.700 | 1000.00 |
28.00 | 0.00 | 100.00 | 0.700 | 1000.00 |
33.00 | 0.00 | 100.00 | 0.700 | 1000.00 |
33.10 | 95.00 | 5.00 | 0.700 | 1000.00 |
40.00 | 95.00 | 5.00 | 0.700 | 1000.00 |
Here, the temperature of the column was maintained at 35 ℃, and 10 μL of the sample was injected into the column. A mass spectrometer was used to analyze ingredients contained in the soybean extract under conditions of mode: ESI(+); mass range: 50 m/z to 800 m/z; nebulizing gas: 8 L/min; source gas temperature: 180 ℃; capillary voltage: +4,500 V; and cone voltage: 35 V.
As a result, the amount of each of epicatechin and procyanidin B2 contained in the selected cultivar named SCEL-1 was in a range of about 0.32% to about 0.46% and in a range of about 1.0% to about 2.0%, respectively, which was significantly higher than the amounts thereof in Wonheug, which were respectively in a range of about 0.14% to about 0.15 % and in a range of about 0.33% to about 0.35%.
In cultivar named SCEL-1 selected on the basis of cyanidin-3-O-glucoside which is a representative substance of anthocyanin antioxidants, cyanidin-3-O-glucoside, procyanidin B2, and epicatechin were contained at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5, for example, 1: 2.0 to 2.1: 0.43 to 0.48. Meanwhile, in Wonheug, cyanidin-3-O-glucoside, procyanidin B2, and epicatechin were contained at a ratio of 1: 0.43 to 0.70 : 0.19 to 0.25.
It should be understood that embodiments described herein should be considered in a descriptive sense only and not for purposes of limitation. Descriptions of features or aspects within each embodiment should typically be considered as available for other similar features or aspects in other embodiments. While one or more embodiments have been described with reference to the figures, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the disclosure as defined by the following claims.
According to the one or more embodiments, a composition for skin health, the composition including an extract of soybean cultivar SCEL-1, may be used to prevent or improve skin wrinkles and/or skin inflammation.
According to the one or more embodiments, a method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, or preventing occurrence of skin inflammation in a subject or improving skin inflammation in a subject may be used to prevent occurrence of skin wrinkles in a subject or improve skin wrinkles in a subject, or to prevent skin inflammation in a subject or improve skin inflammation in a subject.
Claims (13)
- A composition for skin health, the composition comprising, as an active ingredient,an extract obtained by performing an extraction process on a seed of soybean Glycine max (L.) Merrill cultivar SCEL-1 by using water, C1-C6 alcohol, or a mixture thereof, wherein the seed contains cyanidin-3-O-glucoside, procyanidin B2, and epicatechin, wherein an amount of the procyanidin B2 is greater than that of the cyanidin-3-O-glucoside, and a representative sample of the seed is deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018.
- The composition of claim 1, wherein a total amount of cyanidine-3-O-glucoside, procyanidine B2, and epicatechin in the extract is in a range of 2.1 % to 3.4 % based on a total weight of the extract.
- The composition of claim 2, wherein the extract contains cyanidin-3-O-glucoside, the procyanidin B2, and the epicatechin at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5 based on a weight basis.
- The composition of claim 1, wherein an amount of the extract is in a range of 0.005 % to 99.9 % based on a total weight of the composition.
- The composition of claim 1, wherein the skin health includes prevention of skin wrinkles or improvement of skin wrinkles.
- The composition of claim 1, wherein the composition is food, a cosmetic composition, or a pharmaceutical composition.
- A composition for skin health, the composition comprising, as an active ingredient,cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, procyanidin B2 or a physiologically acceptable salt thereof, and epicatechin or a physiologically acceptable salt thereof at a ratio of 1: 0.8 to 2.1: 0.43 to 1.5 on a weight basis, respectively.
- The composition of claim 7, wherein an amount of each of the cyanidin-3-O-glucoside or a physiologically acceptable salt thereof, the procyanidin B2 or a physiologically acceptable salt thereof, and the epicatechin or a physiologically acceptable salt thereof is in a range of 0.005 % to 99.9 %.
- The composition of claim 7, wherein the skin health includes prevention of skin wrinkles or improvement of skin wrinkles.
- The composition of claim 7, wherein the composition is food, a cosmetic composition, or a pharmaceutical composition.
- The composition of claim 7, wherein the composition comprises an extract obtained by performing an extraction process on a seed of soybean cultivar by using water, C1-C6 alcohol, or a mixture thereof, wherein a representative sample of the soybean cultivar seed is deposited with the Korean Agricultural Culture Collection(KACC) under the accession number KACC 88002BP on November 8, 2018.
- A method of preventing occurrence of skin wrinkles in a subject or improving skin wrinkles in a subject, the method comprisingadministering the composition of claim 1 to a subject.
- The method of claim 12, wherein the administration is applied locally to the skin.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020180078246A KR102101790B1 (en) | 2018-07-05 | 2018-07-05 | Composition for skin health comprising Glycine max (L.) Merrill variety SCEL-1 seed extract |
KR10-2018-0078246 | 2018-07-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2020009538A1 true WO2020009538A1 (en) | 2020-01-09 |
Family
ID=69060174
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2019/008302 WO2020009538A1 (en) | 2018-07-05 | 2019-07-05 | Composition for skin health including extract of seed of new soybean cultivar scel-1 |
Country Status (2)
Country | Link |
---|---|
KR (1) | KR102101790B1 (en) |
WO (1) | WO2020009538A1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20220043660A (en) | 2020-09-29 | 2022-04-05 | (주)아모레퍼시픽 | New soybean variety comprising high content of epicatechin |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20050111667A (en) * | 2004-05-21 | 2005-11-28 | 주식회사 엠디바이오알파 | Soybean extract of enhancing biosynthesis of hyaluronic acid and glycosaminoglycan |
US8435541B2 (en) * | 2010-09-02 | 2013-05-07 | Bath & Body Works Brand Management, Inc. | Topical compositions for inhibiting matrix metalloproteases and providing antioxidative activities |
KR20140048258A (en) * | 2011-08-05 | 2014-04-23 | 스템테크 인터내셔널, 인크. | Skin care compositions containing combinations of natural ingredients |
KR101613546B1 (en) * | 2015-08-31 | 2016-04-19 | 서울대학교산학협력단 | Compositions for preventing or improving skin wrinkles comprising soybean extract and Haematococcus extract |
KR20170003400A (en) * | 2015-06-29 | 2017-01-09 | 한국화학연구원 | Nanoparticles that encapsulated with anti-wrinkles active ingredient, preparation method thereof and anti-wrinkles cosmetic composition |
-
2018
- 2018-07-05 KR KR1020180078246A patent/KR102101790B1/en active IP Right Grant
-
2019
- 2019-07-05 WO PCT/KR2019/008302 patent/WO2020009538A1/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20050111667A (en) * | 2004-05-21 | 2005-11-28 | 주식회사 엠디바이오알파 | Soybean extract of enhancing biosynthesis of hyaluronic acid and glycosaminoglycan |
US8435541B2 (en) * | 2010-09-02 | 2013-05-07 | Bath & Body Works Brand Management, Inc. | Topical compositions for inhibiting matrix metalloproteases and providing antioxidative activities |
KR20140048258A (en) * | 2011-08-05 | 2014-04-23 | 스템테크 인터내셔널, 인크. | Skin care compositions containing combinations of natural ingredients |
KR20170003400A (en) * | 2015-06-29 | 2017-01-09 | 한국화학연구원 | Nanoparticles that encapsulated with anti-wrinkles active ingredient, preparation method thereof and anti-wrinkles cosmetic composition |
KR101613546B1 (en) * | 2015-08-31 | 2016-04-19 | 서울대학교산학협력단 | Compositions for preventing or improving skin wrinkles comprising soybean extract and Haematococcus extract |
Non-Patent Citations (2)
Title |
---|
FUKAMI, H. ET AL.: "Isolation of a reduced form of cyanidin 3-0-beta-D- glucoside from immature black soybean (Glycine max (L.) Merr.) and its reducing properties", JOURNAL OF OLEO SCIENCE, vol. 62, no. 8, 2013, pages 623 - 629, XP055673200 * |
ZHAO, R. ET AL.: "Extracts from Glycine max (soybean) induce elastin synthesis and inhibit elastase activity", EXPERIMENTAL DERMATOLOGY, vol. 18, no. 10, October 2009 (2009-10-01), pages 883 - 886, XP055673204 * |
Also Published As
Publication number | Publication date |
---|---|
KR102101790B1 (en) | 2020-04-20 |
KR20200005055A (en) | 2020-01-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2014193014A1 (en) | Nano-sized kimchi lactic acid bacteria | |
KR101502687B1 (en) | Anti-aging and Anti-inflammatory and Anti-oxidant Cosmetic Composition including Beans Placenta Cell Cultures Extracts | |
WO2014182044A1 (en) | Composition for preventing or treating symptoms of menopause comprising soybean extract comprising coumestrol as an active ingredient | |
WO2018062751A1 (en) | Composition containing lysate of lactobacillus plantarum for hair or scalp | |
WO2018164369A1 (en) | Composition for improving skin wrinkles, containing enzyme-treated hibiscus syriacus extract as active ingredient | |
WO2020040432A1 (en) | Pharmaceutical composition for preventing or treating muscle diseases, containing ginseng berry extract as active ingredient | |
WO2020009538A1 (en) | Composition for skin health including extract of seed of new soybean cultivar scel-1 | |
WO2018080039A1 (en) | Composition for preventing hair loss or improving hair growth, containing yellow-colored soybean leaf extract | |
WO2019225786A1 (en) | Antibacterial and antifungal composition comprising extract from clove, hibiscus, and coconut as effective ingredient | |
WO2020009290A1 (en) | Composition for preventing or improving inflammation including extract of seed of new soybean cultivar scel-1 | |
WO2020004739A1 (en) | Seed of new soybean cultivar scel-1, plant body of the seed and part of the plant body, and extract obtained from the seed | |
WO2016056780A1 (en) | Composition for hair loss prevention or hair growth stimulation comprising scutellaria alpina extract | |
US20170135948A1 (en) | Composition comprising extract of autumn soybean leaves | |
WO2021080298A1 (en) | Composition containing enteroccocus faecalis as active ingredient for preventing or treating obesity or metabolic syndromes induced thereby | |
KR102367027B1 (en) | Antimicrobial composition comprising Hydrangea petiolaris extracts or fractions thereof as effective component | |
WO2009107984A2 (en) | Composition for preventing skin aging containing plant sprout extracts | |
WO2020055171A1 (en) | Cosmetic composition for ameliorating wrinkles and increasing elasticity comprising phragmites communis fermentation extract | |
WO2016003120A1 (en) | Whitening composition comprising scutellaria alpina extract | |
WO2023055221A1 (en) | Composition comprising oat extract for alleviating hair loss and promoting hair growth | |
WO2019078646A2 (en) | Composition, containing extract from bean culture, for prevention of hair loss or promotion of hair growth and method for preparing same | |
WO2021167269A1 (en) | Whitening composition comprising plum seed extract as active ingredient | |
WO2023282528A1 (en) | Skin whitening composition comprising extract from white leaf of saururus chinensis (lour.) baill | |
WO2023043111A1 (en) | Scalp microbiome complex and use thereof for improving hair or scalp condition | |
WO2022114783A1 (en) | Composition containing fermented product of cactus honey and use thereof for improving skin condition | |
WO2022114784A1 (en) | Composition comprising fermentation product of cactus oil and use thereof for improving skin condition |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 19831389 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 19831389 Country of ref document: EP Kind code of ref document: A1 |