WO2019227714A1 - Animal swimming test device and test method - Google Patents

Abstract

Disclosed are an animal swimming test device and a test method, the animal swimming test device comprising a plurality of transparent test barrels (1); the animal swimming test device further comprises: a camera (2), a filtration device (3) and a circulation device (4); the circulation device (4) is configured to communicate two adjacent transparent test barrels (1) and to drive water in the two adjacent transparent test barrels (1) to be circularly filtered by the filtration device (3).

Description

Animal swimming experimental device and method

This application claims priority from a Chinese patent application with a filing date of May 29, 2018, application number 201810533117.4, and titled "An Animal Swimming Experimental Device and Method", the entire contents of which are incorporated herein by reference. in.

Technical field

The present disclosure relates to the field of animal experiments, for example, to an animal swimming experimental device and an experimental method.

Background technique

The Forced Swimming Test (FST) system is mainly used for the research of antidepressant, sedative and analgesic drugs. This system is mainly suitable for rats, mice or other laboratory animals. By placing experimental animals in a confined water environment, animals struggle to escape in this environment and cannot escape, thus providing an unavoidable After oppressing the environment, after a period of time, the animals show a typical "rest state", reflecting a "behavioral despair state". During the "rest state" in which the animals in the environment produce despair, a series of parameters are recorded. The rest time The shorter the stronger the antidepressant effect. The animal model of depression with forced swimming is a reliable experimental model for studying the pharmacology and pathogenesis of human depression and screening and observing antidepressants.

In the related art, the forced swimming device is a single transparent cylindrical bucket. After completing an experimental animal's swimming experiment, animal feces and hair will remain in the water, resulting in strong animal odor. These residual odors will affect the next batch of experiments. The behavioral behavior of animals when forced to swim will affect the results of the whole experiment. Therefore, whenever a forced swimming experiment of an experimental animal is completed, a water change operation is required. That is, the bucket is lifted for the water change operation, and then the bucket is returned. This process is not only a waste of time and water resources, but also tedious and prolonged The experiment cycle. On the other hand, the compulsory swimming devices commonly used in related technologies are designed for single barrels, and multiple experimental animals cannot be tested at the same time.

Summary of the Invention

The present disclosure proposes an animal swimming experimental device and an experimental method. The device can perform swimming experiments of multiple animals at the same time, and does not require frequent water changing operations. The experimental method of the experimental method has a shortened experimental period and simple operation.

An animal swimming experimental device includes: a plurality of transparent experimental barrels, each of which is configured to contain water and animals;

A camera is disposed outside the transparent experimental barrel, and the camera is disposed opposite to the side wall of the transparent experimental barrel to record and record the behavior of the animal in the transparent experimental barrel;

A filtering device, which is connected between two adjacent transparent experiment barrels through a pipeline; and

A circulation device configured to communicate with two adjacent transparent experimental buckets and drive water in the two adjacent transparent experimental buckets to pass through the filtering device for circular filtration.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic structural diagram of an animal swimming experiment apparatus provided in Embodiment 1; FIG.

2 is a schematic structural diagram of an animal swimming experiment apparatus provided in Embodiment 2;

FIG. 3 is a flowchart of an animal swimming experiment method provided in Example 3. FIG.

In the picture:

10, pipeline; 20, support;

1. Transparent experiment bucket; 11. Water outlet pipe; 12. Water inlet pipe;

2. Camera;

3. Filtration device; 31. Box body; 32. Activated carbon bag;

4. Circulating device; 40. Pipe; 41. Vacuum pump;

5. Experimental bench; 51. Working surface; 52. Upright

6. Bucket cover; 61. Vias.

Detailed ways

Embodiment one:

FIG. 1 is a schematic structural diagram of an animal swimming experiment apparatus provided in Embodiment 1 of the present disclosure. The experimental apparatus includes a transparent experiment barrel 1, a camera 2, a filtering device 3, and a circulation device 4. The transparent experiment barrel 1 is used to contain water and animals. Since the experiment barrel 1 is transparent material, the camera 2 can be used to record and record the behavior of the animals in the transparent experiment barrel 1 from the outside. The camera 2 can be held by hand or fixed by a stand. There are at least two transparent experimental barrels 1, so that the experimental device can test multiple animals at the same time, which is more efficient. In this embodiment, as shown in FIG. 1, two transparent experiment buckets 1 are provided; the filtering device 3 is connected between two adjacent transparent experiment buckets 1 through a pipe 10, and the circulation device 4 and a plurality of transparent experiment buckets. 1 communicates and can drive the water in multiple transparent experiment buckets 1 to circulate through the filtering device 3 for filtering, so that the experimental water does not need to be replaced frequently, and the operation is simpler.

In one embodiment, the filtering device 3 includes a box body 31 and an activated carbon bag 32. The two ends of the box body 31 are connected to two adjacent transparent experiment barrels 1 through a pipeline 10, and a switching valve is provided on the pipeline 10. The box body 31 can be opened and closed, the activated carbon bag 32 can be replaced, and the activated carbon bag 32 is placed in the box body 31. Filters are also provided on both sides of the activated carbon bag 32, and the filters can be replaced. The replacement of the activated carbon bag 32 and the filter can be set according to the actual situation. In one embodiment, after the experimental device is performed five times, the activated carbon bag 32 and the filter need to be replaced once.

In one embodiment, the circulation device 4 includes a vacuum pump 41. An inlet end of the vacuum pump 41 is inserted into one transparent experiment barrel 1 through a tube 40, and an outlet end of the vacuum pump 41 is inserted into another transparent experiment barrel 1 through a tube 40. In an embodiment, the transparent experimental bucket 1 is further provided with a bucket cover 6. The bucket cover 6 is placed on the transparent experimental bucket 1 and a via hole 61 is provided on the bucket cover 6. A tube 40 connected to the vacuum pump 41 passes through the via hole. 61 and inserted into the transparent experiment barrel 1. The barrel cover 6 makes the setting of the circulation device 4 more reasonable. That is, the barrel cover 6 is first placed on the transparent experimental barrel 1 when in use, and the barrel cover 6 is used as a component carrying the circulation device 4.

The use process of the animal swimming experimental device provided in the first embodiment is as follows:

Fill the transparent experiment bucket 1 with water, close the on-off valves on the pipeline 10 at the first end and the second end of the box 31, put the animals into the transparent experiment bucket 1, and the camera 2 records the animals from the outside in the transparent experiment bucket In the behavior of 1 during the experiment, animal waste such as hair and feces will remain in the water. After the experiment is completed, the opening and closing valves on the first and second ends of the pipe 10 of the box body 31 are opened, and the barrel lid 6 is placed on the transparent experiment barrel 1. The inlet end of the vacuum pump 41 is inserted into the transparent experiment barrel through the tube 40. 1. The outlet end of the vacuum pump 41 is inserted into another transparent experiment bucket 1 through a pipe 40, and the vacuum pump 41 is started to circulate water in the two transparent experiment buckets 1. The water flows from a transparent experimental barrel 1 through the action of the activated carbon bag 32 and the filter screen in the filtering device 3 to remove animal excreta and hair from the water, and filter the water. After the water flow circulates for a certain time, the vacuum pump 41 is turned off, and the circulation device 4 and the bucket cover 6 are taken out. At this time, the water in the two transparent experimental buckets 1 meets the experimental requirements, and the next experiment can be performed. After the experiment was performed about five times, the water in the two transparent experimental buckets 1 had been contaminated, and the filtering device 3 could no longer function as a filter. At this time, the water in the two transparent experimental buckets 1 must be drained and replaced with new ones. At the same time, the box 31 is opened, and the activated carbon bag 32 and the filter are replaced. Among them, the water in the transparent experimental bucket 1 can be drained in the following two ways: lift the transparent experimental bucket 1 and pour off the water, or replace it through the circulation device 4, for example, connect the outlet of the vacuum pump 41 in the circulation device 4 The tube 40 is placed in the water tank, the vacuum pump 41 is started, and the water in the transparent experimental bucket 1 is pumped out and discharged into the water tank.

The animal swimming experimental device provided in the first embodiment can test multiple animals at the same time, which is more efficient, and the experimental water does not need to be replaced frequently, and the operation is simpler.

When the experimental animal is a mouse, the diameter of the transparent experimental barrel 1 is 25 cm-35 cm. In one embodiment, the diameter of the transparent experimental barrel 1 is 20 cm. The height of the transparent experimental barrel 1 is 35 cm-45 cm. In one embodiment, The height of the transparent experimental barrel 1 is 40 cm.

Embodiment two:

FIG. 2 is a schematic structural diagram of an animal swimming experiment apparatus provided in Embodiment 2 of the present invention. As shown in FIG. 2, unlike Embodiment 1, in order to realize the water inlet and water change of the transparent experimental bucket 1, no manual operation is required, and a manual operation is also provided. There are 5 test benches.

As shown in FIG. 2, the experimental table 5 includes a working surface 51 and a plurality of columns 52. Two transparent experimental barrels 1 are placed on the working surface of the experimental table 5. A water outlet pipe 11 is provided on the bottom surface of each transparent experimental barrel 1. The corresponding on-off valve 11 is provided with an on-off valve (not shown in the figure). In an embodiment, the working surface of the experimental table 5 is configured to include a plurality of beams, so that the outlet pipe 11 provided on the bottom surface of the transparent experimental barrel 1 can pass through the gap on the working surface and protrude to the bottom of the working surface. A water inlet pipe 12 is also provided above the experimental table 5 for replenishing the transparent experimental bucket 1 with water. A camera 2 is fixedly disposed on one side of the experimental table 5 through a bracket 20. The camera 2 is photographed toward the side wall of the transparent experimental barrel 1, and the height of the bracket 20 is adjustable.

The use process of the animal swimming experimental device provided in the second embodiment is different from the first embodiment in that:

The water injection operation of the transparent experimental bucket 1 in the second embodiment is implemented by a water inlet pipe 12 provided above the experimental table 5, and the drainage operation is performed by a water outlet pipe 11 provided on the bottom surface of the transparent experimental bucket 1. The arrangement of the water outlet pipe 11 avoids the inconvenience of manually replacing the water or using the circulating device 4 in Embodiment 1. Opening the valve on the water outlet pipe 11 can facilitate drainage.

An animal swimming experimental device provided by the present disclosure enables the experimental device to test multiple animals at the same time, which is more efficient; and a circulation device 4 is provided to drive the water circulation, and the water is filtered by the filtering device 3, so that the experimental water is used. No frequency replacement is required, and operation is simpler.

Example three:

This third embodiment provides an animal swimming experiment method, which may use any one of the experimental devices of the first or the second embodiment. This embodiment uses the experimental device of the second embodiment. The number of transparent experimental barrels is set to two. Mice are examples of antidepressant effects for experimental purposes. As shown in Figure 3, this experimental method mainly includes:

In S10: Two mice are placed in two transparent experimental buckets filled with water, respectively. The temperature of the water in the two transparent experimental barrels was around 25 ° C, which forced the mice to swim.

In S20: The timer is started, and the camera takes pictures of the mice in the transparent experiment barrel.

In S30: when the first predetermined time is reached, the time when the mouse is at rest in the first predetermined time is recorded.

In S40: The mouse is removed, the mouse is dried, and the mouse is returned to the cage.

In this step, the experimental device in Example 2 was used to circulate and filter the water in the two transparent experimental barrels, and the odor, hair, and feces generated during the experiments of the mice in the first step were filtered.

In S50: at a second predetermined time, the two mice in step S40 are respectively put into two transparent experimental buckets filled with water, so that the mice are forced to swim.

In S60: The timer is started, and the camera shoots the mice in the transparent experiment barrel.

In S70: at a third predetermined time interval, the time when the mouse is at rest for the third predetermined time is recorded.

In S80: the mouse is removed.

In the same operation as in step S40, the water in the two transparent experimental buckets is circulated and filtered.

The software was used to analyze the time during which the mice remained still in the transparent experimental barrel in the above steps, and based on this, the problem of antidepressant effects was studied.

The first predetermined time is 13min-17min, in one embodiment the first predetermined time is 15min; the second predetermined time is 15h-30h, and in one embodiment the second predetermined time is 24h, which is an interval of one day; the third predetermined time It is 3min-7min, and in an embodiment, the third predetermined time is 5min.

An animal swimming experiment method provided by the present disclosure is capable of performing experiments on multiple animals at the same time, shortening the experiment cycle and simple operation.

The animal swimming experiment method provided by the present disclosure has a shortened experimental period and simple operation.

Claims (11)

1. An animal swimming experimental device includes:

   Multiple transparent experiment buckets (1), each transparent experiment bucket (1) is set to contain water and animals;

   A camera (2) is disposed outside the transparent experimental barrel (1), and the camera (2) is disposed opposite to a side wall of the transparent experimental barrel (1) to capture and record animals in the transparent experimental barrel (1) );

   A filtering device (3), which is connected between two adjacent transparent experiment barrels (1) through a pipe (10); and

   Circulating device (4), the circulating device (4) is arranged to communicate with two adjacent transparent experimental buckets (1) and drive water in the adjacent two transparent experimental buckets (1) to pass through the filtering device (3) Perform circulating filtration.
2. The animal swimming experimental device according to claim 1, further comprising an experimental table (5), the number of the transparent experimental barrels (2) is two, and the two transparent experimental barrels (1) are both placed in the experiment A water outlet pipe (11) is arranged on the bottom surface of each transparent experiment bucket (1) on the platform (5).
3. The animal swimming experiment apparatus according to claim 2, wherein the camera (2) is disposed on one side of the experimental table (5), and the camera (2) faces a side of the transparent experimental barrel (1) wall.
4. The animal swimming experimental device according to claim 1, wherein the filtering device (3) comprises a box body (31) and an activated carbon bag (32), the box body (31) can be opened and closed, and the box body ( The first end of 31) is connected to one of two adjacent transparent experiment barrels (1) through a pipe (10), and the second end of the box body (31) is connected to an adjacent one through a pipe (10) The other of the two transparent experimental barrels (1) is connected, the activated carbon bag (32) is placed in the box body (31), and the activated carbon bag (32) is arranged to be replaceable.
5. The animal swimming experiment apparatus according to claim 4, wherein a filter screen is further provided on both sides of the activated carbon bag (32), and the filter screen is arranged to be replaceable.
6. The animal swimming experimental device according to claim 1, wherein the circulation device (4) comprises a vacuum pump (41) and a tube (40), and an inlet end of the vacuum pump (41) is inserted into two adjacent two through a tube (40). One of the transparent experiment buckets (1), and the outlet end of the vacuum pump (41) is inserted into another of the two transparent experiment buckets (1) through a pipe (40).
7. The animal swimming experiment apparatus according to claim 6, wherein the transparent experimental bucket (1) comprises a bucket lid (6), and the bucket lid (6) is disposed on the transparent experimental bucket (1), and the The barrel cover (6) is provided with a through hole (61), and a tube (40) connected to the vacuum pump (41) passes through the through hole (61) and is inserted into the transparent experimental barrel (1).
8. The experimental device for animal swimming according to claim 1, wherein the diameter of the transparent experimental barrel (1) is 25cm-35cm, and the height of the transparent experimental barrel (1) is 35cm-45cm.
9. An animal swimming experiment method includes:

   Put multiple animals into multiple transparent experiment buckets filled with water, respectively;

   The timing starts, and the camera shoots the animals in the transparent experiment barrel;

   When the first predetermined time is reached, recording the time when the animal is at rest in the first predetermined time;

   Remove the animal

   Placing the plurality of animals into a plurality of transparent experimental buckets filled with water, respectively, at a second predetermined time;

   The timing starts, and the camera shoots the animals in the transparent experiment barrel;

   A third predetermined time interval, and recording the time when the animal is still in the third predetermined time;

   Remove the animal.
10. The animal swimming experiment method according to claim 9, wherein the first predetermined time is 13min-17min, the second predetermined time is 15h-30h, and the third predetermined time is 3min-7min.
11. The animal swimming experiment method according to claim 9, further comprising the step of circulating filtering the water in the plurality of transparent experimental buckets.

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