WO2019198238A1 - Méthode de culture de micro-algue - Google Patents

Méthode de culture de micro-algue Download PDF

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Publication number
WO2019198238A1
WO2019198238A1 PCT/JP2018/015594 JP2018015594W WO2019198238A1 WO 2019198238 A1 WO2019198238 A1 WO 2019198238A1 JP 2018015594 W JP2018015594 W JP 2018015594W WO 2019198238 A1 WO2019198238 A1 WO 2019198238A1
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WO
WIPO (PCT)
Prior art keywords
microalgae
growth
liquid medium
adjustment tank
period
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PCT/JP2018/015594
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English (en)
Japanese (ja)
Inventor
長尾　宣夫
戸田　龍樹
松山　達
モハメド ユソフ，ファティマ
シャリフ モハメド ディン，モハメド
Original Assignee
学校法人 創価大学
ユニバーシティー プトラ マレーシア
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Application filed by 学校法人 創価大学, ユニバーシティー プトラ マレーシア filed Critical 学校法人 創価大学
Priority to JP2019560422A priority Critical patent/JP6741284B2/ja
Priority to PCT/JP2018/015594 priority patent/WO2019198238A1/fr
Publication of WO2019198238A1 publication Critical patent/WO2019198238A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Definitions

  • the present invention relates to a method for culturing microalgae.
  • microalgae for absorption of carbon dioxide generated at power plants, factories, etc., domestic wastewater, sewage treatment such as sewage. Since the microalgae have a high growth rate, they are used as raw materials for biofuels, fertilizers, feeds, and the like, as well as high value-added ones used for medicines, nutritional foods, cosmetic raw materials and the like.
  • High-value-added microalgae are vulnerable to contamination and are conventionally cultured in a liquid medium contained in a growth container using a closed growth container configured to be isolated from the outside air.
  • the microalgae fix carbon in the air by photosynthesis and generate oxygen, the oxygen concentration in the growth vessel increases when the growth is active.
  • the microalgae will breathe as a reverse reaction of photosynthesis, and absorb oxygen and release carbon, so the oxygen in the growth vessel is removed. It is preferable to do.
  • a vertical growth vessel is used as the closed growth vessel, and aeration is performed from the bottom of the growth vessel to agitate the liquid medium and simultaneously remove oxygen generated by photosynthesis of the microalgae.
  • the vertical growth vessel has a problem that there is a limit to the number of microalgae that can be cultivated per unit, making it difficult to culture in large quantities.
  • a large number of vertical growth vessels can be used to cultivate microalgae in large quantities.
  • the energy required for stirring, aeration, deaeration, etc. is excessive for the unit weight of the microalgae yield. There is a problem of becoming.
  • the method of culturing microalgae using a horizontal closed growth vessel has the disadvantage that the depth of the liquid medium is limited due to light permeability, and it is difficult to agitate the liquid medium and remove oxygen by aeration.
  • An object of the present invention is to provide a method for culturing microalgae that can eliminate such inconvenience and can stir a liquid medium and remove oxygen even when a horizontal closed growth vessel is used. .
  • the method for culturing microalgae of the present invention comprises the following steps (1) to (4), wherein step (1) is performed during a predetermined growth period, and step (2) ) To step (4), and after performing step (1), at least steps (2) to (4) are performed in a predetermined adjustment period, and the growth period and the adjustment period are repeated.
  • Step (1) Microalgae are placed in a closed growth container configured to be isolated from the outside air, and grown in a photoautotrophic manner in a liquid medium contained in the growth container.
  • Step (2) At least a part or all of the liquid medium on which the microalgae are grown in the step (1) is transferred from the growth vessel to the adjustment tank together with the microalgae.
  • Step (3) The mixture of the microalgae and the liquid medium transferred to the adjustment tank in the step (2) is degassed in the adjustment tank.
  • Step (4) The mixture of the microalgae degassed in the step (3) and the liquid medium is transferred from the adjustment tank to the growth vessel.
  • the microalgae are grown in a photoautotrophic manner in a liquid medium contained in the growth vessel. If it does in this way, in the said growth container, carbon will be fix
  • the microalgae will breathe as a reverse reaction of photosynthesis, and absorb oxygen and release carbon. Therefore, after growing the microalgae as described above, in the step (2) as the adjustment period, at least a part or all of the liquid medium is transferred from the growth vessel to the adjustment tank together with the microalgae. To do.
  • dissolved oxygen in the liquid medium is obtained by degassing the mixture of the microalgae and the liquid medium transferred to the adjustment tank in the adjustment tank in the step (3). Reduce concentration.
  • the step (4) the mixture of the microalgae and the liquid medium whose dissolved oxygen concentration is reduced by the degassing treatment is transferred from the adjustment tank to the growth vessel. And the said process (1) as the said growth period is performed again.
  • the microalgae can be grown in a photoautotrophic manner again in the growth container.
  • the transfer of the mixture of the microalgae and the liquid medium is intermittently repeated between the growth vessel and the adjustment tank, Can be stirred.
  • the liquid medium can be stirred and oxygen can be removed even when a closed growth vessel is used.
  • the method for culturing microalgae of the present invention can be applied even when the growth vessel is a vertical type, but is particularly preferably applied when the growth vessel is a horizontal type container arranged in a horizontal direction. Can do.
  • the transition period from the growth period to the adjustment period may be after the growth period has been performed for a predetermined time, but the liquid medium in which the microalgae are grown It is preferable to determine the concentration based on the dissolved oxygen concentration.
  • the microalgae respirate as a reverse reaction of photosynthesis, and absorb oxygen and release carbon. Therefore, by determining the transition time from the growth period to the adjustment period based on the dissolved oxygen concentration of the liquid medium, it is possible to shift from the growth period to the adjustment period at an appropriate time.
  • the microalgae be grown photoautotrophically by suspending the growth vessel below the surface of water such as lakes and rivers or below the sea level.
  • a large area can be easily secured for installing the growth vessel, while the growth vessel is in water with a large heat capacity, so the microalgae and the liquid medium in the growth vessel It is possible to prevent the temperature of the mixture from rising excessively.
  • the effect that the mixture of the said micro algae in the said growth container and the said liquid culture medium can also be acquired by the wave motion of the water surface or the sea surface.
  • the growth container when the growth container is suspended below the water surface or the sea surface, the growth container has an opening at one end, a part thereof is made of a stretchable material, and the adjustment is performed via a transfer pipe connected to the opening.
  • the mixture is communicated with the tank, and the mixture of the microalgae and the liquid medium is transferred to the adjustment tank by its own weight by allowing the adjustment tank to settle so that the bottom of the adjustment tank is lower than the opening. It is preferable to transfer the mixture of the microalgae and the liquid medium to the growth vessel by its own weight by raising the adjustment tank so that the bottom of the adjustment tank is positioned higher than the opening.
  • the mixture of the microalgae and the liquid medium can be transferred simply by raising and lowering the adjustment tank with respect to the opening, and the energy required for the transfer can be reduced.
  • a space is provided above the liquid medium accommodated in the growth container, and a carrier gas is circulated in the space, so that the microalgae accumulate in the space due to the growth of the microalgae. It is preferable to discharge oxygen.
  • the number of the growth containers may be one, but a plurality may be provided.
  • Explanatory drawing which shows the initial state of transfer in one Embodiment of the cultivation method of the micro algae of this invention.
  • Explanatory drawing which shows the state in the middle of transfer in one Embodiment of the cultivation method of the micro algae of this invention.
  • Explanatory drawing which shows the completion
  • Explanatory drawing which shows other embodiment of the cultivation method of the micro algae of this invention.
  • the method for culturing microalgae of the present invention comprises placing a high-value-added microalgae used in medicines, nutritional foods, cosmetic raw materials, etc. in a closed growth container configured to be isolated from the outside air, Is a culture method for photoautotrophic growth in a liquid medium accommodated in the medium.
  • Examples of the high-value-added microalgae include Haematococcus genus such as Haematococcus pluvialis, Haematococcus lacustris, Chlorella vulgaris, Chlorella sorokiniana, Chlorella ofzofingiensis genus Chlorella, Nannochloropsis Dunaliella genus, Botryococcus braunii, etc., Botryococcus genus such as Botryococcus braunii, other green algae, Isochrysis ⁇ galbana, Isochrysis litoralis etc. , Cyanobacterium including Arthrospira genus such as Arthrospira maxima, Euglena genus such as Euglena gracilis, and the like.
  • the growth vessel may be any material that is made of a light-transmitting material so that the microalgae grow light-autotrophically.
  • a material such as hard polyvinyl chloride, acrylic resin, or glass. What has been configured can be used.
  • the liquid medium those containing nutrients for growing the microalgae photoautotrophically can be used, for example, fresh water medium such as BBM, BG-11, F / 2, Conway, etc.
  • artificial media such as seawater media
  • sewage containing nutrients of microalgae such as nitrogen can be used.
  • the method for culturing microalgae of the present invention can be carried out, for example, by a system using a bag reactor 1 suspended under the water surface S as a growth vessel, as shown in FIG. 1A.
  • the bag reactor 1 is a bag-like container made of a stretchable material such as soft polyvinyl chloride, polyolefin, and fluororesin, and is disposed horizontally in the horizontal direction directly below the water surface S.
  • the bag reactor 1 contains a liquid medium 2 therein, and cultivates microalgae in the liquid medium 2.
  • the bag reactor 1 has an opening at one end, and a transfer pipe 3 is connected to the opening.
  • the other end of the transfer pipe 3 is connected to the bottom of the adjustment tank 4, and as a result, the bag reactor 1 is connected to the adjustment tank 4 via the transfer pipe 3.
  • the adjustment tank 4 includes a cylindrical upper part and a conical bottom part connected to the upper part, and is configured to be able to sink or rise with respect to the water surface S.
  • the microalgae are grown in a photoautotrophic manner in the liquid medium 2 accommodated in the bag reactor 1. This step corresponds to step (1) in the growth period of claim 1.
  • the dissolved oxygen concentration in the liquid medium 2 increases, and when it exceeds the limit, the microalgae breathe as a reverse reaction of photosynthesis and absorb oxygen. Then carbon is released. Therefore, if the dissolved oxygen concentration in the liquid medium 2 exceeds a predetermined range, at least a part or all of the liquid medium 2, for example, 2/3, is mixed with the microalgae as a mixture of the liquid medium 2 and the microalgae. Transfer from the bag reactor 1 to the adjustment tank 4. This step corresponds to step (2) in the adjustment period of claim 1.
  • the microalgae are grown in the bag reactor 1 for a predetermined time.
  • the dissolved oxygen concentration of the liquid medium 2 can be detected, for example, by disposing a water quality sensor equipped with a dissolved oxygen meter or the like in the middle of the transfer tube 3.
  • the water quality sensor may include a pH meter, a turbidity meter, a pressure meter, a salt concentration meter, a thermometer, etc. in addition to the dissolved oxygen meter.
  • the mixture of the liquid medium 2 and the microalgae is transferred from the bag reactor 1 to the adjustment tank 4 by allowing the adjustment tank 4 to settle as shown in FIG. 1B.
  • the adjusting tank 4 is allowed to settle so that the bottom of the adjusting tank 4 is positioned lower than the opening of the bag reactor 1
  • the mixture in the bag reactor 1 enters the adjusting tank 4 through the transfer pipe 3 by its own weight. Be transported.
  • the bag reactor 1 contracts as the mixture is transferred, and can absorb a decrease in volume due to the transfer of the mixture.
  • the transfer of the mixture from the bag reactor 1 to the adjustment tank 4 is completed when most of the mixture in the bag reactor 1 is transferred to the adjustment tank 4 as shown in FIG. 1C.
  • the degassing treatment may be performed by aeration such as aeration in the adjustment tank 4 or may be performed by making the upper space of the mixture in the adjustment tank 4 have a negative pressure. As a result, the dissolved oxygen concentration in the liquid medium 2 can be reduced to the limit, for example.
  • the mixture is then transferred from the adjustment tank 4 to the bag reactor 1. This step corresponds to step (4) in the adjustment period of claim 1.
  • the transfer of the mixture from the adjustment tank 4 to the bag reactor 1 can be performed by raising the adjustment tank 4 from the state shown in FIG. 1C. If it does in this way, the said mixture which becomes a position higher than the opening of the bag reactor 1 in the adjustment tank 4 will be transferred in the bag reactor 1 via the transfer pipe 3 with the dead weight.
  • the transfer pipe 3 is the same as the transfer pipe 3 used when transferring the mixture from the bag reactor 1 to the adjustment tank 4.
  • the mixture is transferred from the adjustment tank 4 to the bag reactor 1 by raising the bottom of the adjustment tank 4 to a position higher than the opening of the bag reactor 1, and most of the mixture in the adjustment tank 4 is transferred to the bag reactor 1. To finish.
  • the microalgae Since the dissolved oxygen is reduced in the liquid medium 2 contained in the mixture transferred to the bag reactor 1, the microalgae starts photoautotrophic growth again.
  • the mixture is transferred from the bag reactor 1 to the adjustment tank 4 and transferred again from the adjustment tank 4 to the bag reactor 1, whereby the liquid medium 2 contained in the mixture is sufficiently stirred, Algae can grow well.
  • the liquid medium 2 can be agitated and oxygen can be removed by repeating the growth period and the adjustment period.
  • the bag reactor 1 is described as being suspended below the water surface S. However, the bag reactor 1 may be suspended below the sea surface. By suspending the bag reactor 1 under the water surface S or the sea surface, a large area can be easily secured for installing the bag reactor 1.
  • the method for culturing microalgae of the present invention can be carried out by a system in which a plurality of bag reactors 1 are arranged on the ground.
  • a bag reactor 1 shown in FIG. 2 is a bag-like container made of the stretchable material, and is disposed horizontally in the horizontal direction on the ground.
  • a plurality of bag reactors 1 are shown to be arranged vertically, but the plurality of bag reactors 1 can be arranged radially around the adjustment tank 4, for example.
  • the size of the bag reactor 1 can be appropriately set according to the volume of the adjustment tank 4, but the depth of the liquid medium 2 accommodated therein is preferably set to 100 mm or less from the light transmittance.
  • Each bag reactor 1 accommodates a liquid medium 2 and is connected to the bottom of the adjustment tank 4 via a transfer pipe 3.
  • the adjustment tank 4 shown in FIG. 2 is hermetically sealed, and the vacuum pump 5 disposed at the top is operated to discharge the internal gas, and the internal pressure is reduced to at least a part of the liquid medium 2 or All, for example, 2/3 can be transferred from the bag reactor 1 to the adjusting tank 4 as a mixture of the liquid culture medium 2 and the microalgae together with the microalgae.
  • the mixture is degassed by aeration by aeration or the like, or by making the upper space of the mixture in the adjustment tank 4 have a negative pressure.
  • carbon dioxide may be added to the mixture as a carbon source, and the amount of carbon dioxide added is a water quality sensor disposed in the middle of the transfer pipe 3, and the mixture detected by the water quality sensor. It can be determined based on the carbon dioxide concentration in the medium.
  • the temperature of the mixture may be adjusted by a charging temperature controller.
  • the bottom of the adjustment tank 4 is positioned higher than each bag reactor 1.
  • the operation of the vacuum pump 5 is stopped and the outside air is introduced into the adjustment tank 4, whereby the mixture in the adjustment tank 4 is transferred to the bag reactor 1 through the transfer pipe 3 by its own weight. Can be transported.
  • the transfer pipe 3 is the same as the transfer pipe 3 used when transferring the mixture from the bag reactor 1 to the adjustment tank 4.
  • the microalgae can be cultured in the same manner as the system shown in FIG. 1, and the liquid medium 2 is stirred by repeating the growth period and the adjustment period. And oxygen can be removed.
  • a space 6 is provided above the liquid culture medium 2 in the bag reactor 1, a gas introduction pipe 7 a that introduces air as a carrier gas into the space 6, and a gas that discharges the carrier gas from the space 6.
  • a discharge pipe 7b can also be provided.
  • the dissolved oxygen concentration in the liquid medium 2 can be reduced in the bag reactor 1 during the growth period, and the growth period can be further increased, while the mixture from the bag reactor 1 to the adjustment tank 4 is increased.
  • the number of times of transfer can be reduced.
  • the bag reactor 1 is a horizontal growth container arranged in the horizontal direction.
  • the bag reactor 1 may be a vertical growth container arranged in the vertical direction. Good.
  • 1 bag reactor (growth vessel), 2 ... liquid medium, 4 ... regulation tank.

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Abstract

L'invention concerne une méthode de culture de micro-algue avec laquelle même lorsqu'un récipient de croissance fermé horizontal est utilisé, un milieu liquide peut être agité et de l'oxygène peut être retiré de celui-ci. Pendant une période de croissance, une micro-algue est cultivée photoautotrophiquement dans un milieu liquide 2 contenu dans un réacteur à poche fermé 1. Dans une période d'ajustement, le milieu liquide 2 dans lequel la micro-algue s'est développée est transféré du réacteur à poche 1 à un réservoir d'ajustement 4 avec la micro-algue à l'intérieur de celui-ci, est soumis à un traitement de dégazage dans le réservoir d'ajustement 4, puis est transféré du réservoir d'ajustement 4 au réacteur à poche 1. La période de croissance et la période d'ajustement sont répétées.
PCT/JP2018/015594 2018-04-13 2018-04-13 Méthode de culture de micro-algue WO2019198238A1 (fr)

Priority Applications (2)

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JP2019560422A JP6741284B2 (ja) 2018-04-13 2018-04-13 微細藻類の培養方法
PCT/JP2018/015594 WO2019198238A1 (fr) 2018-04-13 2018-04-13 Méthode de culture de micro-algue

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WO2019198238A1 true WO2019198238A1 (fr) 2019-10-17

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59156279A (ja) * 1983-02-28 1984-09-05 Takashi Mori 光合成反応装置
JPH09121835A (ja) * 1995-10-27 1997-05-13 Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko チューブラ型フォトバイオリアクタ
JP2005040035A (ja) * 2003-07-25 2005-02-17 Mitsubishi Heavy Ind Ltd バイオリアクタ
JP2013162762A (ja) * 2012-02-10 2013-08-22 Sumitomo Heavy Ind Ltd 光合成微生物培養装置
JP2015053896A (ja) * 2013-09-12 2015-03-23 本田技研工業株式会社 バイオマス用反応装置
JP2017006090A (ja) * 2015-06-25 2017-01-12 アルジー グローバル センター プロプライアタリー リミティド 藻類回収装置、ならびに藻油を製造するためのシステム及び方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013085534A (ja) * 2011-10-20 2013-05-13 Eco Renaissance Entec:Kk 微細藻類の培養方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59156279A (ja) * 1983-02-28 1984-09-05 Takashi Mori 光合成反応装置
JPH09121835A (ja) * 1995-10-27 1997-05-13 Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko チューブラ型フォトバイオリアクタ
JP2005040035A (ja) * 2003-07-25 2005-02-17 Mitsubishi Heavy Ind Ltd バイオリアクタ
JP2013162762A (ja) * 2012-02-10 2013-08-22 Sumitomo Heavy Ind Ltd 光合成微生物培養装置
JP2015053896A (ja) * 2013-09-12 2015-03-23 本田技研工業株式会社 バイオマス用反応装置
JP2017006090A (ja) * 2015-06-25 2017-01-12 アルジー グローバル センター プロプライアタリー リミティド 藻類回収装置、ならびに藻油を製造するためのシステム及び方法

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JP6741284B2 (ja) 2020-08-19

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