WO2019180749A2 - Dispositif tridimensionnel portatif additif automatique à plusieurs étages pour la fabrication d'organes durs et mous - Google Patents

Dispositif tridimensionnel portatif additif automatique à plusieurs étages pour la fabrication d'organes durs et mous Download PDF

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WO2019180749A2
WO2019180749A2 PCT/IR2018/050024 IR2018050024W WO2019180749A2 WO 2019180749 A2 WO2019180749 A2 WO 2019180749A2 IR 2018050024 W IR2018050024 W IR 2018050024W WO 2019180749 A2 WO2019180749 A2 WO 2019180749A2
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Prior art keywords
injection
bio
nozzles
design
chamber
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PCT/IR2018/050024
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English (en)
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WO2019180749A3 (fr
WO2019180749A4 (fr
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Sayedali MOUSAVI
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Mousavi Sayedali
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Priority to CA3094496A priority Critical patent/CA3094496A1/fr
Publication of WO2019180749A2 publication Critical patent/WO2019180749A2/fr
Publication of WO2019180749A3 publication Critical patent/WO2019180749A3/fr
Publication of WO2019180749A4 publication Critical patent/WO2019180749A4/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/112—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using individual droplets, e.g. from jetting heads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/118—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using filamentary material being melted, e.g. fused deposition modelling [FDM]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
    • B29C64/25—Housings, e.g. machine housings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

Definitions

  • This Invention relates to a bio printing field for cloning hard and soft organs and scaffold surface treatment .
  • This invention also relates to methods of using different nozzles and actuators to construct special shaped organs.
  • Tissue engineering and regenerative medicine is implementing for alleviating of organ shortages to improve patient’s serious conditions.
  • bio printers are using bio ink such as hydrogel with cells in three dimensional shapes to direct cells to for growth and integrations to special tissue.
  • bio ink such as hydrogel with cells in three dimensional shapes to direct cells to for growth and integrations to special tissue.
  • These systems are using 3 degree freedom additive manufacturing systems technologies by printing layer by layer. There are several restriction for these technologies such as precise multiple cell placement, low density cell purity ,less cell interaction, prolonged material degradation for bio film, scaffold toxicity , separation of cells in one porous scaffold, separated medium transfer for specific cells for differentiation.
  • Bio printer chamber limited time of printing due to cell vitality there is still a further need to concur these defects.
  • Described herein are a bioprinter with four degree of freedom with four printing head and isolated chamber with support assembly and the controller for sequence of printing and condition monitoring.
  • the controller is configured with three methods via computer, manually by panel and with Wi-Fi feature thro Mobile application.
  • injection materials such as hydro gel, bio polymer, composite with four external infusions injection pumps each layer of tissue would be fabricated.
  • the placement of multiple cell type in porous construct, each type of cells could be isolated via electro spinning fibred scaffold and the microfluidic tunnels could be specifically designed to reach the specific media to these cells for cell maturation.
  • the injection nozzles assembled in rotated disc is receiving raw materials such as bio inks and bio polymers filament and dispense them according to printing pattern.
  • Electro spinning nozzle and composite nozzle could be added to disc.
  • Electro spinning system is used for nano fiber based scaffold generation system.
  • low powers laser irradiator with 800nm wavelet actuator for surface treatment for decrease cell adhesion and vitality and also increase proliferation.
  • Plasma arc deposition actuator for scaffold hydrophobic purpose is used to isolate each tissue segment and covey the media for special cells for maturation and differentiation.
  • the nozzle assembly printer head have coaxial mechanism so it have inner and outer chamber to inject fluid with drug and gels components with cells simultaneously.
  • parameters such as set temperature body is set to be 37 degree centigrade, CO2 gas serve to maintain pH to natural 7.4, similar to tension in blood stream, high humidity to evaporation of media of (85-95%), for proper culturing and increase cell vitality in long process of printing.
  • the controller set s and the heater elements and fan could control heat by heat transfer process and thermal sensor is used in close loop control system.
  • Co2(0-20%) and air(O2-N2) the air injection pump with controlling output flow of Co2 and air, were used to maintain the parameters related to set points .
  • coaxial nozzles instead of a single-pump nozzle can be used to inoculate the drug, as well as the injection of hydrogels like alginate and calcium chloride injections to fix and cross-link the hydrogel with controlling UV, heat condition.
  • the use of a single or multiple nozzles submersible pump is printing in a single unit inside the container, but the four injection pumps of this invention are located outside the of the body bio printer .
  • the four injection pumps of this invention are located outside the of the body bio printer .
  • Differences and Advantages of this innovation is it does not require the opening and closing of the enclosure door to replace the injected fluid and control the parameters of the external environment in the operator's function and observation with long printing time, which is an innovative application of this statement.
  • Another advantage of this invention is that in general there is no mechanism for modifying the structure with low powered plasma and laser diffusion surface treatment during printing. This feature would increase cell vitality and integrations.
  • the one single-layer body was integrated with an integrated chamber, is provided.
  • One of the advantages of the invention come from a double-glazed body, the use of composite and ABS polymer in internal and external structures with an internal strengthening shell to increase the structural stability and create
  • the closed environment is designed to create an incubator environment inside the chamber and has been designed and manufactured in a very distinct and more functional form with a more static and ergonomic design for better user access.
  • the two instantaneous coolant control items are intended, and the invention of the gradual cooling mechanism is used.
  • Another benefit of the incubation environment is that the control of the conditions of the cell culture media is adjusted to the input according to the culture conditions of each cellular tissue.
  • three heat elements are installed in the device, one on the bottom of chamber on in heat sink on middle, and one on the top of extruder, which creates heat. Both the one is mounted on the extruder of the filament and one on the back of the device, which is different in terms of the mechanism of transmission and temperature reduction.
  • Creating an incubator environment inside the printer chamber increases the print time from 30 minutes due to the death of stem cells for several hours.
  • coaxial nozzles instead of a single-pump nozzle can be used to inoculate the drug, as well as the injection of hydrogels like alginate and calcium chloride injections to fix and cross-link the hydrogel with controlling UV, heat condition.
  • the use of a single or multiple nozzles submersible pump is printing in a single unit inside the container, but the four injection pumps of this invention are located outside the of the body bio printer .
  • the four injection pumps of this invention are located outside the of the body bio printer .
  • Differences and Advantages of this innovation is it does not require the opening and closing of the enclosure door to replace the injected fluid and control the parameters of the external environment in the operator's function and observation with long printing time, which is an innovative application of this statement.
  • Another advantage of this invention is that in general there is no mechanism for modifying the structure with low powered plasma and laser diffusion surface treatment during printing. This feature would increase cell vitality and integrations.
  • the one single-layer body was integrated with an integrated chamber, is provided.
  • One of the advantages of the invention come from a double-glazed body, the use of composite and ABS polymer in internal and external structures with an internal strengthening shell to increase the structural stability and create
  • the closed environment is designed to create an incubator environment inside the chamber and has been designed and manufactured in a very distinct and more functional form with a more static and ergonomic design for better user access.
  • the two instantaneous coolant control items are intended, and the invention of the gradual cooling mechanism is used.
  • Another benefit of the incubation environment is that the control of the conditions of the cell culture media is adjusted to the input according to the culture conditions of each cellular tissue.
  • three heat elements are installed in the device, one on the bottom of chamber on in heat sink on middle, and one on the top of extruder, which creates heat. Both the one is mounted on the extruder of the filament and one on the back of the device, which is different in terms of the mechanism of transmission and temperature reduction.
  • Creating an incubator environment inside the printer chamber increases the print time from 30 minutes due to the death of stem cells for several hours.
  • aspects of this invention consist of two distinct but related aspect 1 and 2.
  • Aspect 1 is the bio-printer mechanism and the aspect 2 is injection pump mechanism.
  • bio printer mechanism comprising :four degree of freedom three linear movement with lead and screw mechanism coupled to stepper motor and containing rotating disk that connected to stepper motor ,double layer printing chamber with incubation parameters , nozzles ,extruder ,Actuators and rotating disk .
  • the hydrogel components with cells, bio composite and drug will be controlled by the velocity injection pump mechanism.
  • These hydrogel injection nozzles are pumped cells and chemical crosslinking stabilizing solution.
  • aspect 1 for injection of hydrogels, nano particles and drugs, a variety of polymer solutions and various types of fluids such as media, growth factors, stabilizers, other factors could be used.
  • simple four-injection pumps mechanism is used to increase the rate, pressure and flow rate with regard to the sequence of printing and type of density of materials for printing.
  • Aspect 2 is injection pumps of any of aspects 22-28 , wherein there is positive pump pressure inside housing witch is in range 1 -20 kPa.
  • All movements are powered by four 1.8-degree, 4.5 watt stepper motors, with a total accuracy of 0.00027 mm that can be moved around all axes.
  • the main controller of the device based on the definition process, gives the commands to the motors for the displacement of the plates (three axes) or the rotary transmission mechanism to change the injection and correction mechanisms of the Disk and plate.
  • biodegradable polymers such as PVA,PLGA-PLA-PLLA,PCL,PETG.
  • the temperature and speed of the polymer injection are adjusted according to the type of polymer of the filament. The best result was obtained with a nozzle of 0.2 and 0.1 and a temperature of about 40 to 50 degrees Centigrade. The temperature of the element and the environment was reduced by the fan at top of extruder.
  • the Fan with nano filter is used to decrease the temperature of chamber and it could operate from 0 to 100 percent performance that could be controlled by operator from computer program.
  • the filament injection mechanism act as extruder it dragged and moved by the stepper motor.
  • Motor Stepping angel and its movement are influences the different filament injection rates. Using too little speed when printing with these polymers may cause the material in the filament to obstruct and jam in the extruded outlet.
  • controller unit and power supply located at down of structural box and all electrical input and output would connect to this part.
  • the incubator chamber control system that control temperature, humidity and amount of input Co2 is located in this part.
  • rotating disk unit All attachments and nozzles are attached on disk to create and inject fluids layer by layer scaffolds.
  • this rotating disk could be substitute with another rotating disk that contains actuators such as laser and atmospheric plasma tesla mechanisms.
  • actuators such as laser and atmospheric plasma tesla mechanisms.
  • On the rotating Disk with screw mechanism there is ability to add or remove the nozzles .
  • linear transmutation system that contain motor that coupled to power screw in the x, y axis transfer this mechanism of transmission of disk with nozzles and on a single sheet is carried out.
  • the mechanism of the hydro gel nozzle is used for inject combination of living cells (the types of differentiated stem cells or cells) and the components of hydrogels and a variety of new substrates that are liquid. Creating compound scaffolds is difficult to keep the layers created together.
  • the hydrogel injection nozzles are of the coaxial type, they can be mix hydrogel components with along with the growth factors and all media.
  • the second category involves the use of low-level laser spray nozzles and low-level atmospheric plasma emitter nozzles to improve the structure of the scaffolds, which will be further elaborated.
  • Atmospheric plasma atomic nozzles break down the surface bonds, and by releasing hydrogen bonds and levels of abstinence, the cells grow and propagate the differentiated stem cells).
  • the length of the plasma arc is 1-6 cm, with adjustable magnetic field and electrical conductivity of 5-20 kV. This produces a pair of positive-charge ions and negatively charged electrons at the surface and causes hydrophobic levels of these surfaces.
  • nozzles and actuators are connected to it aspect 8, 9,11,12 .
  • the first is the scaffolding additive manufacturing mechanism that printing the later of scaffolds to shape the organs.
  • the second category is a variety of mechanisms for modifying the surface structure.
  • the first subdivision consists of four injection mechanisms, which are referred to below.
  • the adjustable wavelet for Infrared lamp and ultra violet is located in this area act as light actuator for crosslinking and polymerization of composites and hydro gels.
  • Low-power infrared laser (wavelength 800-950 nm, 200 MW power, radiation dose up to 4 Jules per centimeter, and with the control of the irradiation time and according to the laser tube mounted on the devices can be changed and upgraded.
  • this laser is a potentiometer for adjusting power and output wattage.
  • the driver can be equipped with a variety of diode types with different powers, as well as a TTL control port to control the laser diode's power. It has adjustment of values of power that could stimulate and increase proliferation and integration cells.
  • Low-power laser treatment is effective in the formation of organs.
  • the small fan located at top of extruder that decreases the temperature of heater of extruder to control filament temperature.
  • the connecting rod used for adjust the movement in x axes and control the vibration in the movement is this axes.
  • the next mechanism is the electro spinning mechanism for creating scaffolds containing nano fibers for isolation and differentiation of tissues.
  • This system is a fiber production method which uses electric force to draw charged threads of polymer solutions or polymer melts up to fiber diameters when high voltage is applied to a liquid droplet, the body of the liquid becomes charged, and electrostatic repulsion counteracts the surface tension and the droplet is stretched with a connection to the earth (ground).
  • the movement in Z axis is used by the heat plate bed in which the sample of the tissue is printed bed.
  • the direction of the vertical axis is the motion of the crankshaft, which is raised by the linear bearings and the conductor rod, it decrease vibration of the system and the precision of the transfer is obtained through this structure.
  • this mechanism on injection of polymers in electro spinning is used for the production of nano fibers with core-shell structures.
  • This nozzle is two-sided or coaxial nozzles to inter polymer and drugs made by stainless steel can be auto cleavable.
  • a fluid with a diameter of millimeters or nm will be converted to create a scaffold.
  • the printed scaffold will be connected to the inheritance, and due to the insolubility of the polymers, the possibility of coating on the polymer and composite scaffolding is possible.
  • the power inputs and the system wires are inserted from the bottom of the printer and connected to the controller of the device.
  • variable volumetric syringes is pumped with adjustable output diameters.
  • the output of fluid from injection pump is inserted into the bottom of the printer and then connected to the nozzles with disposable tubes.
  • all nozzles and actuators have the ability to assemble and disassemble, and therefore the number of these devices can be increased and decreased based on the specific printing process.
  • the entire transfer system and nozzles are arranged in an isolated chamber like incubator condition with controlling of amount of temperature e, humanity, carbon dioxide and oxygen. Air intakes and the amount of carbon dioxide is measured and the blower is done on them on chamber via input tube.
  • the interior control of temperature is done with two heater elements that increased and with fan it is decrease. Amount of humidity and oxygen also controlled and it is an essential condition for the survival of cells in the interior of the device during printing.
  • the structure of printer chamber is made by double-glazed compartment.
  • the entire printing program is controlled by computer program, the mobile app and manually.
  • G Codes would be generated for layer by layer formation process.
  • This injection pumps consist of a lead and screw with size 20 millimeter and 50 centimeter long with pitch .08milimeter act as power transmitting for linear movement mechanism with coupling to a stepper motor with a 1.8 degree step angel and 1.65 amperes.
  • syringes of different dimensions can be used. By adding the cap and adjusting the screw to the syringe diameter, these syringes are fixed within the pump mechanism.
  • the entire injection pump mechanism is portable and can be transported with a carrier handle along with a bio-printer.
  • the linear bearing mechanism and guide rod for moving and preventing vibrations are embedded in this system.
  • filament bases is located in top of printer that all rolled filament could inserted to extruder form this area.
  • the 1.8 stepping angel stepper motor is connecting to extruder mechanism to deliver filament to melting chamber .Inside this chamber is sterilize at first of operation with Ultra violet lamp.
  • the present invention provide a incubator chamber contain 3d hybrid bio printer.
  • the incubator chamber is sized such that it is adapted to surround or encompass the hybrid bioprinter while minimizing additional space.
  • the inventive Incubation chamber provide zone of different adjustable percentage amount of O2, N , Co2, Humid air and air surrounding the hybrid bio printer .
  • the controllers of hybrid bio printer and gas and humid air located at under plate the below the chamber isolated plates .
  • the gas and humid air inter chamber with plastic nozzle that located in bottom of chamber that connected to plastic flexible pipe .
  • the temperature of incubator chamber is set to be between 36-38 degree centigrade and the thermal sensor detect temperature and the exact temperature could reach with increase it with heater and decrease it two fans and could control the amount of temperature in chamber.
  • the amount of Co2, O2 ,N is measured by sensor and with insert of these gases throw inlet pipe to nozzle the set amount would reaches and it would increase cell vitality.
  • the hybrid bio printer’s incubator chamber is smaller than 2 m ⁇ 3 with integrated all gas and humid hair supply units.
  • the incubator chamber is on standard laboratory bench witch include hybrid bio printer components inside.
  • this system could accommodate bioprinter isolation chamber of variety of other configuration, non-limiting examples of which are described in U.S. Patent Ns 20110136162 , WO 201740675 and WO 2017/040675A1.
  • the hybrid bio printer contain all components such as , stepper motors, Power movement displacement mechanism, All substrates for printing, nozzles , actuators, laser, plasma, controller, incubator chamber, Door with anti UV coat, Fan ,electrical boards, cables , pipes.
  • Material plasma treated for hydrophobicity is known art, for example U.S 5028453.
  • Micro channel and tunnel is used in micro transfer of media and this system in manufactured by electro spinning and lithography simultaneously.
  • this system could accommodate bioprinter of variety of other configuration , non-limiting examples of which are described in U.S. Patent Ns. 8241905, 9149952, 20130302872.
  • This invention contemplates that these nozzles and actuators can be configured to any bio printer known in art, as well as future improvements in 3D bioprinter technologies.
  • rotating disk is added to bioprinter movement mechanism to change tools such as different size of nozzles, actuators, Ultra Violate and Infra-Red lamps and LED. These tools could be attachable and several injection mechanisms could add to this instrument. These UV and IR could use for photo crosslinking of hydro gels.
  • Different nozzles like coaxial nozzles are used to inject hydrogels and other liquid contents.
  • tissue utilizing UV cross linking are known art, for example PCT/US2013/036479.
  • this system could accommodate moveable tool changing disk of variety of other configuration, non-limiting examples of which are described in KR 101817429 B1.
  • This innovation could use for printing 3 layer skin.
  • the first layer is used by bio polymer and second one is hydrogel with cells and final layer is electro spinning layer that antibiotic drug purified on it.
  • FIG. 1 is a schematic diagram showing a front view of the bio printer technology according to an embodiment of the invention.
  • FIG. 1 is a schematic diagram showing a design of side view of the entire bio-printer system.
  • FIG. 1 is a schematic diagram presentation a design of top view of the entire bio-printer system.
  • FIG. 1 is a schematic diagram illustrates a detailed configuration of the bio printer according to an embodiment of the invention.
  • FIG. 1 is a schematic diagram showing a top view of the injection pump mechanism according to an embodiment of the invention.
  • FIG. 1 is a schematic diagram showing a side view of the injection pump mechanism according to an embodiment of the invention.
  • FIG. 1 is a schematic diagram presenting a front view of the injection pump mechanism according to an embodiment of the invention.
  • FIG. 1 is a schematic diagram illustrates detailed configuration of the injection pumps according to an embodiment of the invention.
  • This invention could use to clone hard and soft organ with surface treatment and could implements in regenerative medicine industry.

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Abstract

L'invention concerne l'utilisation de bio-imprimantes en médecine régénérative ces dernières années. Les problèmes de base de ces inventions sont le manque de résistance mécanique en l'absence de processus complets pour l'injection de différents matériaux avec des cellules distinctes, le manque de stabilisation de la substance biologique (bio-encre) et une faible distribution de cellules uniforme. La solution est la production d'un nouveau dispositif additif portatif qui incorpore des mécanismes de commande précis avec la chambre isolée avec un système de bio-impression hybride pour la production de tissu mou et dur présentant la morphologie de l'organe. Avec un mécanisme de modification structurelle du laser à faible puissance, un plasma augmente également la compatibilité des polymères et augmente l'adhésion et l'intégration des cellules dans les échafaudages.
PCT/IR2018/050024 2018-03-18 2018-08-21 Dispositif tridimensionnel portatif additif automatique à plusieurs étages pour la fabrication d'organes durs et mous WO2019180749A2 (fr)

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CA3094496A CA3094496A1 (fr) 2018-03-18 2018-08-21 Dispositif tridimensionnel portatif additif automatique a plusieurs etages pour la fabrication d'organes durs et mous

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IR13963014980 2018-03-18

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113696477A (zh) * 2021-08-20 2021-11-26 浙江迅实科技有限公司 一种生物3d打印培养一体化设备

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KR102215693B1 (ko) * 2013-10-11 2021-02-15 어드밴스드 솔루션즈 라이프 사이언스, 엘엘씨 바이오 물질 구성물의 설계, 제조 및 조립을 위한 시스템 및 워크스테이션
WO2017023865A1 (fr) * 2015-07-31 2017-02-09 Techshot, Inc. Système de biofabrication, procédé, et matériel de bioimpression 3d dans un environnement à gravité réduite

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113696477A (zh) * 2021-08-20 2021-11-26 浙江迅实科技有限公司 一种生物3d打印培养一体化设备

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