WO2019173695A3 - Analyse de pathogènes multiplex sur micropuce et ses utilisations - Google Patents

Analyse de pathogènes multiplex sur micropuce et ses utilisations Download PDF

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Publication number
WO2019173695A3
WO2019173695A3 PCT/US2019/021317 US2019021317W WO2019173695A3 WO 2019173695 A3 WO2019173695 A3 WO 2019173695A3 US 2019021317 W US2019021317 W US 2019021317W WO 2019173695 A3 WO2019173695 A3 WO 2019173695A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
bottom end
copy number
dna
analysis
Prior art date
Application number
PCT/US2019/021317
Other languages
English (en)
Other versions
WO2019173695A2 (fr
Inventor
Michael Edward Hogan
Melissa Rose MAY
Frederick Henry EGGERS
Original Assignee
Pathogendx, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/916,062 external-priority patent/US11542498B2/en
Priority claimed from US15/916,036 external-priority patent/US10612075B2/en
Priority claimed from US16/158,276 external-priority patent/US10513745B2/en
Priority to KR1020207029030A priority Critical patent/KR20210028142A/ko
Priority to CA3093451A priority patent/CA3093451A1/fr
Priority to JP2020571331A priority patent/JP2021516995A/ja
Application filed by Pathogendx, Inc. filed Critical Pathogendx, Inc.
Priority to MX2020009282A priority patent/MX2020009282A/es
Priority to CN201980030521.2A priority patent/CN112154215A/zh
Priority to AU2019231794A priority patent/AU2019231794A1/en
Priority to EP19764885.0A priority patent/EP3762508A4/fr
Publication of WO2019173695A2 publication Critical patent/WO2019173695A2/fr
Publication of WO2019173695A3 publication Critical patent/WO2019173695A3/fr
Priority to ZA2020/06213A priority patent/ZA202006213B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un système de micropuce à réseau tridimensionnel pour la détection et l'analyse de séquences d'ADN et des kits comprenant des composants de celui-ci. Dans le système, un support solide comporte une pluralité de lieurs polymères bifonctionnels, éventuellement dotés d'une étiquette fluorescente, ayant chacun un domaine supérieur et une extrémité inférieure jointe à celui-ci au niveau de ladite l'extrémité inférieure et une pluralité de sondes d'acide nucléique jointes au domaine supérieur de la pluralité de lieurs polymères bifonctionnels. Un procédé de fabrication du système de micropuce et des procédés pour détecter et identifier des pathogènes dans un échantillon végétal ou pour déterminer le nombre de copies d'un ADN pathogène dans un échantillon d'acide nucléique non purifié à l'aide d'un nombre de copies connu d'ADN synthétique à titre d'étalon interne sont en outre décrits.
PCT/US2019/021317 2018-03-08 2019-03-08 Analyse de pathogènes multiplex sur micropuce et ses utilisations WO2019173695A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP19764885.0A EP3762508A4 (fr) 2018-03-08 2019-03-08 Analyse de pathogènes multiplex sur micropuce et ses utilisations
AU2019231794A AU2019231794A1 (en) 2018-03-08 2019-03-08 Microarray based multiplex pathogen analysis and uses thereof
CN201980030521.2A CN112154215A (zh) 2018-03-08 2019-03-08 基于微阵列的多重病原体分析及其应用
CA3093451A CA3093451A1 (fr) 2018-03-08 2019-03-08 Analyse de pathogenes multiplex sur micropuce et ses utilisations
JP2020571331A JP2021516995A (ja) 2018-03-08 2019-03-08 マイクロアレイベースの多重病原体分析およびその使用
KR1020207029030A KR20210028142A (ko) 2018-03-08 2019-03-08 마이크로어레이 기반 멀티플렉스 병원체 분석 및 이의 용도
MX2020009282A MX2020009282A (es) 2018-03-08 2019-03-08 Análisis de patógenos en formato múltiple basado en microarreglos y usos del mismo.
ZA2020/06213A ZA202006213B (en) 2018-03-08 2020-10-07 Microarray based multiplex pathogen analysis and uses thereof

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US15/916,062 US11542498B2 (en) 2015-12-28 2018-03-08 Microarray based multiplex pathogen analysis and uses thereof
US15/916,062 2018-03-08
US15/916,036 US10612075B2 (en) 2015-12-28 2018-03-08 Microarray based multiplex pathogen analysis and uses thereof
US15/916,036 2018-03-08
US16/158,276 2018-10-11
US16/158,276 US10513745B2 (en) 2015-12-28 2018-10-11 Microarray based multiplex pathogen analysis and uses thereof

Publications (2)

Publication Number Publication Date
WO2019173695A2 WO2019173695A2 (fr) 2019-09-12
WO2019173695A3 true WO2019173695A3 (fr) 2019-12-26

Family

ID=67847534

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2019/021317 WO2019173695A2 (fr) 2018-03-08 2019-03-08 Analyse de pathogènes multiplex sur micropuce et ses utilisations

Country Status (9)

Country Link
EP (1) EP3762508A4 (fr)
JP (1) JP2021516995A (fr)
KR (1) KR20210028142A (fr)
CN (1) CN112154215A (fr)
AU (1) AU2019231794A1 (fr)
CA (1) CA3093451A1 (fr)
MX (1) MX2020009282A (fr)
WO (1) WO2019173695A2 (fr)
ZA (1) ZA202006213B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210317540A1 (en) * 2015-12-28 2021-10-14 Pathogendx, Inc. Microarray-Based Multiplex Fungal Pathogen Analysis
US20240279367A1 (en) * 2020-08-12 2024-08-22 Genemind Biosciences Co., Ltd. Amino-modified chip, preparation method therefor and use thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989011548A1 (fr) * 1988-05-20 1989-11-30 Cetus Corporation Sondes specifiques a des sequences immobilisees
WO1998020019A1 (fr) * 1996-11-06 1998-05-14 Sequenom, Inc. Compositions et procedes d'immobilisation d'acides nucleiques sur des supports solides
WO2003043402A2 (fr) * 2001-10-19 2003-05-30 Proligo Llc Sondes d'acide nucleique et procedes de detection et/ou de quantification d'analytes d'acide nucleique
US20080299578A1 (en) * 2007-05-14 2008-12-04 Canon Kabushiki Kaisha Probe, probe set, probe carrier, and testing method
EP1417337B1 (fr) * 2001-07-11 2009-03-04 Baylor College of Medicine Procedes et dispositifs bases sur une nouvelle forme de double helice d'acide nucleique sur une surface
WO2016203740A1 (fr) * 2015-06-17 2016-12-22 東洋製罐グループホールディングス株式会社 Procédé d'étude de microorganismes, kit d'étude de microorganismes, micropuce pour l'étude de microorganismes, support pour détection de moisissures, procédé de détection de moisissures et kit de détection de moisissures

Family Cites Families (7)

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CA2297661A1 (fr) * 1997-07-22 1999-02-04 Darwin Molecular Corp. Amplification et autres reactions enzymatiques effectuees sur les alignements matriciels d'acides nucleiques
US6238869B1 (en) * 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
WO2000009758A1 (fr) * 1998-08-14 2000-02-24 The Regents Of The University Of California NOUVEL AMPLICON DE LA REGION 20q13 DU CHROMOSOME HUMAIN 20 ET UTILISATIONS DE CELUI-CI
ES2404311T3 (es) * 2005-04-12 2013-05-27 454 Life Sciences Corporation Métodos para determinar variantes de secuencias usando secuenciación ultraprofunda
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EP2532754A1 (fr) * 2011-06-07 2012-12-12 Koninklijke Philips Electronics N.V. Dispositifs et procédés pour la capture efficace d'acides nucléiques
EP2997037B1 (fr) * 2013-05-14 2018-11-21 Genomics USA, Inc. Utilisation d'une composition pour piéger une protéine sur une surface

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989011548A1 (fr) * 1988-05-20 1989-11-30 Cetus Corporation Sondes specifiques a des sequences immobilisees
WO1998020019A1 (fr) * 1996-11-06 1998-05-14 Sequenom, Inc. Compositions et procedes d'immobilisation d'acides nucleiques sur des supports solides
EP1417337B1 (fr) * 2001-07-11 2009-03-04 Baylor College of Medicine Procedes et dispositifs bases sur une nouvelle forme de double helice d'acide nucleique sur une surface
WO2003043402A2 (fr) * 2001-10-19 2003-05-30 Proligo Llc Sondes d'acide nucleique et procedes de detection et/ou de quantification d'analytes d'acide nucleique
US20080299578A1 (en) * 2007-05-14 2008-12-04 Canon Kabushiki Kaisha Probe, probe set, probe carrier, and testing method
WO2016203740A1 (fr) * 2015-06-17 2016-12-22 東洋製罐グループホールディングス株式会社 Procédé d'étude de microorganismes, kit d'étude de microorganismes, micropuce pour l'étude de microorganismes, support pour détection de moisissures, procédé de détection de moisissures et kit de détection de moisissures

Non-Patent Citations (4)

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SHARMA RANJANA ET AL.: "Detection of Transgenic and Endogenous Plant DNA in Digesta and Tissues of Sheep and Pigs Fed Roundup Ready Canola Meal.", J. AGRIC. FOOD CHEM., vol. 54, 2006, pages 1699 - 1709, XP055668933 *
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Also Published As

Publication number Publication date
EP3762508A2 (fr) 2021-01-13
ZA202006213B (en) 2022-01-26
MX2020009282A (es) 2021-01-08
KR20210028142A (ko) 2021-03-11
AU2019231794A1 (en) 2020-10-29
CA3093451A1 (fr) 2019-09-12
CN112154215A (zh) 2020-12-29
JP2021516995A (ja) 2021-07-15
WO2019173695A2 (fr) 2019-09-12
EP3762508A4 (fr) 2021-12-15

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