WO2019170027A1 - 特效复合生物酶制剂 - Google Patents

特效复合生物酶制剂 Download PDF

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Publication number
WO2019170027A1
WO2019170027A1 PCT/CN2019/076407 CN2019076407W WO2019170027A1 WO 2019170027 A1 WO2019170027 A1 WO 2019170027A1 CN 2019076407 W CN2019076407 W CN 2019076407W WO 2019170027 A1 WO2019170027 A1 WO 2019170027A1
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enzyme preparation
biological enzyme
special
composite biological
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PCT/CN2019/076407
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French (fr)
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杨民玉
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山东双圆生物科技有限公司
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Publication of WO2019170027A1 publication Critical patent/WO2019170027A1/zh

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    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes

Definitions

  • the invention belongs to the field of pulp and papermaking, and particularly relates to a special effect composite biological enzyme preparation.
  • the raw material for biological pulping is plant fiber, and the most important part in the production process is the degradation of plant fiber.
  • These raw plant fibers usually include wood fibers, grass fibers, bast fibers, cotton fibers, and the like. Due to the complexity of the plant fiber wall, the degradation of different components requires a variety of enzymes, including simple enzymes and complex enzymes.
  • Enzyme is a kind of biocatalyst. Its application and research have a long history, and people's understanding of some enzymes has reached a certain level. And played a huge role in production yesterday. But so far, most of the enzymes studied for production have been obtained from commons, although they have many advantages, such as higher vigor, ease of preparation, and the like. However, their instability and loss of activity in extreme temperatures, pH, ionic strength, etc., are reduced or even lost, limiting its application.
  • the special-effect composite biological enzyme preparation is considered to be in an environment in which ordinary biological enzymes cannot survive, and can be safe and stable, so it has a very attractive application prospect.
  • the present invention provides a special-effect composite biological enzyme preparation.
  • the self-promoting self-heating special effect compound biological enzyme preparation pulping method has the advantages of energy saving, high efficiency, cleanness, low carbon environmental protection and the like. It is characterized by thermophilic, psychrophilic, acidophilic, and basophilic properties of a special-effect composite biological enzyme preparation. After catalytically or externally heated to a critical temperature of 60-80 ° C, the plant's energy band is quickly broken, and the pectin, tannin, starch, and part of acetic acid and lignin contained in the plant itself are used, except for retaining cellulose.
  • All of the hemicellulose is decomposed and burned as a thermal energy source, thereby realizing the heat required for cooking various plants from the inside and outside without using a pressure vessel for continuously cooking at temperatures above 90 ° C for several hours without external heating.
  • the goal of zero discharge of pulp is fully realized, which is a revolution in the pulp and paper industry and opens up a new way.
  • a special-effect composite biological enzyme preparation consisting of the following raw materials by weight: 1-5 parts of glutamate dehydrogenase, 3-8 parts of citrate synthase, 1-4 parts of catalase, and disproportionation enzyme 3- 8 parts, 1-5 parts of auxiliary enzyme, 70-90 parts of organic acid nutrient solution.
  • the special-effect composite biological enzyme preparation is composed of the following raw materials by weight: 3-5 parts of glutamate dehydrogenase, 5-8 parts of citrate synthase, 2-4 parts of catalase, dismutase 5-8 parts, 3-5 parts of auxiliary enzymes, 82-90 parts of organic acid nutrient solution.
  • the special-effect composite biological enzyme preparation is composed of the following raw materials by weight: 1-3 parts of glutamate dehydrogenase, 3-5 parts of citrate synthase, 1-2 parts of catalase, dismutase 3-5 parts, 1-3 parts of auxiliary enzymes, 70-82 parts of organic acid nutrient solution.
  • the dismutase is 5% of superoxide disproportionated SOD.
  • the auxiliary enzyme is niacinamide.
  • the organic acid nutrient solution is acetic acid.
  • the present application also provides a method for preparing a special-effect composite biological enzyme preparation, which comprises 1-5 parts of glutamate dehydrogenase, 3-8 parts of citrate synthase, 1-4 parts of catalase, and 3 parts of dismutase. -8 parts, 1-5 parts of auxiliary enzyme, 70-90 parts of organic acid nutrient solution are uniformly mixed according to the above ratio, that is, obtained.
  • the present application also provides a method for treating plant material of a special-effect composite biological enzyme preparation, comprising:
  • the mass ratio of the special-effect composite biological enzyme preparation, water and plant material is 0.6:1:4-5.
  • the plant material is eucalyptus or bagasse.
  • the method utilizes a special effect compound biological enzyme preparation to produce. No alkali, no acid, no chlorine, no drainage, no boiler, short pulping time, self-heating, saving coal, electricity and so on.
  • the product produced by the method fully conforms to the chemical pulp standard for papermaking, the hardness is less than 12, and the whiteness is greater than 75 degrees. Especially in the special fiber manufacturing advantages are more prominent. In the high-end field of rice paper, insulation materials, good food fiber, etc., there is a great development prospect.
  • the preparation method has the advantages of simple preparation method, high pulping efficiency, strong practicability and easy promotion.
  • the enzyme activities of the enzymes in the examples of the present application are all greater than 1000 U/g, and each percentage is a mass percentage.
  • the specific preparation method of the special-effect composite biological enzyme preparation of the invention is a composite biological enzyme prepared according to different plant varieties, types, growth years and main chemical components thereof, and products required for the terminal.
  • glutamate dehydrogenase to dehydrogenate to cellulose-based bonds, it can be hydrolyzed to D-glucose; using citrate synthase as an intermediate to break the intercellular layer of plant functional size molecules; using hydrogen peroxide
  • the enzyme can increase the syringyl structural unit of lignin, degrade the quality of hydroxyphenyl, methoxy, phenolic hydroxyl, and small fractions, and enhance the lignin reactivity.
  • the electron transfer key is heated to achieve the purpose of self-heating after activation.
  • the chemical composition of wood is as follows: carbon 49%-50%; hydrogen 6%; oxygen 45%-50%; nitrogen 0.1%-1%; ash: calcium, potassium, sodium, Manganese, iron, phosphorus, sulfur, and silicon in the tropics.
  • Wood composition 1, polysaccharides: cellulose and hemicellulose; 2, lignin (belonging to aromatic compounds); 3, extractables: resin, volatile oils, tannins, pigments, etc.; Ash (mineral)
  • the wood special effects compound biological enzyme preparation pulping is as follows:
  • citrate synthase There are three main types in the application: (1), citrate synthase; (2) glutamate dehydrogenase; (3) catalase.
  • citrate synthase There are three main types in the application: (1), citrate synthase; (2) glutamate dehydrogenase; (3) catalase.
  • citrate synthase There are three main types in the application: (1), citrate synthase; (2) glutamate dehydrogenase; (3) catalase.
  • the ethyl group of acetyl-CoA is condensed with the keto group of oxaloacetate to form cityl-CoA, so that the subsequent high-energy sulfate bond is hydrolyzed, and Coenzyme A is released to obtain citrate synthase.
  • Citric acid synthase is a regulatory enzyme that regulates the metabolism of plant fat, the oxidation of fatty acids and the hydrolysis of cells, and plays an important role.
  • the biochemical characteristics of citrate synthase, physical specificity, reaction catalyzes the condensation of acetyl-CoA with oxaloacetate to form a citric acid complex enzyme.
  • the most suitable pH and the most suitable reaction temperature, citrate complex enzyme and acetate coenzyme are relatively stable in the range of pH 6.0-8.0 according to their localization in plant eukaryotic cells.
  • the pH of the electrogenic model bacteria is 8.0, and the most suitable reaction temperature is 30-55 degrees.
  • citrate complex enzymes currently found are generally homodimers or hexamers. Usually divided into two categories.
  • Gram-positive archaea and eukaryotic cells Bacillus subtilis, electrogenic model bacteria and aquatic torogen, while Gram-positive bacteria are generally homologous hexamers, and both Acetobacter and Nitrosomonas are homologous hexamers.
  • the peroxisomes of plants and some fungi may be derived from eubacteria.
  • citric acid complex enzyme plays an important role in plant metabolism, it forms a stable complex. These complexes play an important role in plant, increase the activity of organic acid synthase gene and increase the synthesis and decomposition of organic acids.
  • Glutamate dehydrogenase which is ubiquitous in plants, is not a major component of plant nitrogen use, but plays an important role in plant nitrogen metabolism.
  • Nicotinamide adenine dinucleotide is a coenzyme. It is assimilated by ammonium in the senescence process of plants under high temperature and water stress. Features. However, under dark or carbon stress conditions, ammonium oxide can be formed to form an organic acid, thereby providing a carbon skeleton for the tricarboxylic acid cycle.
  • the wood chemical component provided generally has 1% nitrogen in the plant; 49% hydrogen and 48% oxygen.
  • the invention utilizes glutamate dehydrogenase to produce 1% ammonium in a citric acid complex enzyme under high temperature catalysis to form an organic tricarboxylic acid, thereby making a tricarboxylic acid and catalase, hydrogen peroxide Deuteration reaction.
  • the three substances of hydrogen, oxygen and nitrogen in plants are transformed. Most of the hydrogen atoms are used as a heat source, and a part of oxygen and hydrogen atoms generate water.
  • Catalase is an enzyme that catalyzes the decomposition of hydrogen peroxide into oxygen and water. Its main function is to catalyze the decomposition of hydrogen peroxide into water and oxygen.
  • the hydrogen peroxide is prevented from reacting with oxygen, and under the action of the chelating compound, the iron particles react to form a substance harmful to the production of cellulose, causing the cellulose to turn yellow. There is no effect on the cellulose, so the hydrogen peroxide remaining on the bleached fibers is thoroughly hydrolyzed to prevent further oxidation of the cellulose.
  • the pH value is 6-8
  • the temperature is 20-55 ° C, but mixed with organic acid, hydrogen peroxide, special-effect composite biological enzyme preparation
  • the general pH value is 1.5-5.5
  • the temperature is 80-90 ° C, the decomposition of hydrogen peroxide is accelerated. It plays a key role and is unique to the present invention.
  • the two enzymes are Shanghai Keshun Biotechnology, which are sold by Xi'an and other companies.
  • the price is 99-220 yuan / kg, the root needs are different, the price is not the same.
  • Catalase and dismutase and other excipients are available nationwide.
  • Each single enzyme gene has the characteristics of being the most suitable for the reaction temperature of the enzyme, good thermal stability and fast reaction speed;
  • the developed composite enzyme preparation has clear component components and known action sites, which can effectively prevent the formation of by-products and the occurrence of unanticipated reactions;
  • the proportion of each enzyme component of the compound enzyme preparation can be compounded in an ideal ratio, and the synergistic effect of the enzyme preparation can be maximized, and the inhibition reaction of some single enzyme preparations in the natural enzyme can be effectively improved;
  • High temperature catalytic conditions can significantly reduce the viscosity of the reactants and reduce the power consumption cost of the production process
  • High temperature catalytic conditions are effective to prevent contamination of bacteria, improve product quality, and facilitate the production of later processes
  • the solubility of the reaction substrate can be increased, the reaction volume can be reduced, and the process operating conditions can be simplified;
  • Part of the enzyme preparation can also be used in an organic reaction catalytic system.
  • the novel composite biological enzyme studied by the invention has great market prospects;
  • the preparation is colorless, non-toxic, natural organic and environmentally friendly
  • the specific pulping method is as follows:
  • Viscosity measurement according to GB/T 1548-2004 standard, determined by copper ethylene diamine method; Kappa number: determined according to GB/T 1546-2004 standard;
  • the above-mentioned eucalyptus pulp was copied into paper according to 60g/m 2 , and the whiteness was measured: after the slurry sample was disintegrated by the fiber disintegrator, it was printed on a Buchner funnel, and after air drying, YQ-Z-48A type whiteness color was used. The meter measures the whiteness.
  • the test results showed that the kappa number of the eucalyptus pulp was 11.5, the viscosity was 651.2 ml/g, and the whiteness of the pulp was 80.3% ISO.
  • the specific pulping method is as follows:
  • Viscosity measurement according to GB/T 1548-2004 standard, determined by copper ethylene diamine method; Kappa number: determined according to GB/T 1546-2004 standard;
  • the above bagasse pulp was copied into paper according to 60g/m 2 , and the whiteness was measured: after the pulp sample was disintegrated by the fiber disintegrator, it was printed on a Buchner funnel, and after air drying, it was determined by YQ-Z-48A whiteness color. The instrument measures whiteness.

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  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
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Abstract

一种特效复合生物酶制剂及一种特效复合生物酶制剂的处理植物原料的方法，该特效复合生物酶制剂由如下重量份的原料组成：谷氨酸脱氢酶1-5份、柠檬酸合成酶3-8份、过氧化氢酶1-4份、歧化酶占3-8份，辅助酶1-5份，有机酸营养液70-90份。该特效复合生物酶制剂的处理植物原料的方法，包括：1)将植物原料干燥、称重、切片，待用；2)将特效复合生物酶制剂和水按比例加入到上述植物原料中，混合均匀，于密闭条件下经催化缴活或外加热至临界温度60-80℃后，反应一段时间。

Description

特效复合生物酶制剂 技术领域
本发明属于制浆造纸领域，特别涉及特效复合生物酶制剂。
背景技术
目前采用生物法制浆的原料是植物纤维，在生产过程中最重要的环节就是对植物纤维进行降解。这些原料植物纤维通常包含木材纤维、草类纤维、韧皮纤维、棉麻纤维等。由于其中植物纤维壁的复杂性，降解其中的不同组份就需要各种不同的酶，包括简单酶类和复合酶类。
酶是一种生物催化剂，它的应用和研究已有很长的历史，人们对一些酶的认识也达到了一定的水平。并在生产中发挥了巨大的昨用。但是到目前为止，所研究的用于生产的酶大多数都是从普通物中得到，尽管它们有许多优点，如活力较高，制备容易等。但是，它们在极端的温度，pH值、离子强度等环境中的不稳定和活力下降，甚至丧失，限制了它的应用。特效复合生物酶制剂被认为普通生物酶无法生存的环境中，可以保特活力和稳定，所以有十分诱人的应用前景。
发明内容
为此，本发明提供一种特效复合生物酶制剂。采用自催自加热特效复合生物酶制剂制浆法，该方法具有节能、高效、清洁、低碳环保等优点。它是利用特效复合生物酶制剂所具有的嗜热、嗜冷、嗜酸、嗜碱等特性。经催化缴活或外加热至临界温度60-80℃后，迅速打破植物的管能团，利用植物自身所含的果胶、单宁、淀粉、和部分乙酸、木质素，除保留纤维素、半纤维素外全部作为热能源分解燃烧，从而实现了不用外加热可持续数小时连续不断在90℃以上蒸煮，在不用压力容器的条件下，从里向外的蒸煮各种植物所需的热量，完全实现制浆零排放目标，对制浆造纸行业是一次革命，开辟了一条新的途径。
为了实现上述目的，本发明采用如下技术方案：
一种特效复合生物酶制剂，由如下重量份的原料组成：谷氨酸脱氢酶1-5份、柠檬酸合成酶3-8份、过氧化氢酶1-4份、歧化酶占3-8份，辅助酶1-5份，有机酸营养液70-90份。
优选的，所述特效复合生物酶制剂，由如下重量份的原料组成：谷氨酸脱氢酶3-5份、柠檬酸合成酶5-8份、过氧化氢酶2-4份、歧化酶占5-8份，辅助酶3-5份，有机酸营养液82-90份。
优选的，所述特效复合生物酶制剂，由如下重量份的原料组成：谷氨酸脱氢酶1-3份、柠檬酸合成酶3-5份、过氧化氢酶1-2份、歧化酶占3-5份，辅助酶1-3份，有机酸营养液70-82份。
优选的，所述的歧化酶为超氧化物歧化SOD占5％。
优选的，所述辅助酶为烟酰胺。
优选的，所述有机酸营养液为醋酸。
本申请还提供了一种特效复合生物酶制剂的配制方法，将谷氨酸脱氢酶1-5份、柠檬酸合成酶3-8份、过氧化氢酶1-4份、歧化酶占3-8份，辅助酶1-5份，有机酸营养液70-90份按上述比例混合均匀，即得。
本申请还提供了一种特效复合生物酶制剂的处理植物原料的方法，包括：
1)将植物原料干燥、称重、切片，待用；
2)将上述特效复合生物酶制剂和水按比例加入到上述植物原料中，混合均匀，于密闭条件下经催化缴活或外加热至临界温度60-80℃后，反应一段时间，即得。
优选的，所述特效复合生物酶制剂、水和植物原料的质量比为0.6：1：4-5。
优选的，所述植物原料为桉木或蔗渣。
本发明的有益效果
(1)工艺流程短。传统工艺：一、备料工序：二、蒸煮工序：三、喷仓：四、碱回收、五,洗浆：六漂白：七、锅炉：八水处理。新工艺：一、备料：二、反应器：三、洗浆调质：四、水循环利用工序。二、投资少：比传统工艺节约一半，生产成本低，无三废：占地少等。不用投资碱回收，锅炉、大型水处理厂。三本方法对各种木、竹、韧皮类、麻、草类植物都能适用。适应性广，一次性投资可以生产各种产品，在不改变工艺的前提下，只需改变配方可以生产各种高、中、低产品。和利用各种原材料。四、本方法利用特效复合生物酶制剂生产。不用碱、不用酸、不用氯、不排水、不用锅炉、制浆时间短、自行加热、节约煤、电等优点。本方法生产的产品，完全符合造纸用化学浆标准，硬度小于12以下，白度大于75度。特别在特种纤维制造优点更加突出。在宣纸、绝缘材料、善食纤维等，高端领域具有很大的发展前途。
(2)本发明制备方法简单、成浆效率高、实用性强，易于推广。
具体实施方式
应该指出，以下详细说明都是例示性的，旨在对本申请提供进一步的说明。除非另有指 明，本文使用的所有技术和科学术语具有与本申请所属技术领域的普通技术人员通常理解的相同含义。
需要注意的是，这里所使用的术语仅是为了描述具体实施方式，而非意图限制根据本申请的示例性实施方式。如在这里所使用的，除非上下文另外明确指出，否则单数形式也意图包括复数形式，此外，还应当理解的是，当在本说明书中使用术语“包含”和/或“包括”时，其指明存在特征、步骤、操作、器件、组件和/或它们的组合。
本申请实施例中各酶的酶活皆大于1000U/g，各百分比皆为质量百分比。
下面结合具体的实施例，对本发明做进一步的说明。
实施例1
本发明特效复合生物酶制剂具体制备方法是，根据不同植物品种、种类、生长年限和其主要化学组分，以及终端所需要产品来进行制备的复合生物酶。
利用谷氨酸脱氢酶在氢离子催化下，脱去纤维素基键，可以水解到D-葡萄糖；利用柠檬酸合成酶做中间体，打破植物官能团大小分子的胞间层；利用过氧化氢酶能增加对木素的紫丁香基结构单元，对羟苯基、甲氧基、酚羟基，以及小分质量降解，增强木质素反应活性。经歧化反应，电子传递键加热，达到经激活后，自催自加热的目的。
根据一般植物的化学组分，像木材的化学组分如下：碳49％-50％；氢6％；氧45％-50％；氮0.1％-1％；灰分中：钙、钾、钠、锰、铁、磷、硫，热带地区含硅等。木材的成分：1、多糖类：纤维素和半纤维素；2、木素(属于芳香族化合物质)；3、可提取物：树脂、挥发性油类、单宁、色素等；4、灰分(矿物质)
根据以上说明列举，木材特效复合生物酶制剂制浆配比如下：
谷氨酸脱氢酶3％、柠檬酸合成酶5％、过氧化氢酶2％、歧化酶占5％，辅助酶3％，有机酸营养液82％。
一、复合酶液的具体成你：
在申请中有主要有三种：分别是(1)、柠檬酸合成酶；(2)、谷氨酸脱氢酶；(3)、过氧化氢酶。下面分做一下说明：一、柠檬酸合成酶：
在三羧酸循环第一步反应中，催化乙酰辅酶A的乙基与草酰乙酸的酮基缩合生成柠檬酰辅酶A，以便后续高能硫酸键水解，释放出辅酶A，得到柠檬酸合成酶。
柠檬酸合成酶是一个调控酶，能调控植物脂肪的代谢，脂肪酸的氧化及细胞的水解，起着重要作用。
柠檬酸合成酶的生物化学特性，物理特异性，反应催化乙酰辅酶A与草酰乙酸缩合生成拧檬酸复合酶。
最适合的pH值和最适合的反应温度，柠檬酸复合酶和乙酸辅酶，根据其在植物真核细胞中的定位，在pH值6.0-8.0的范围内比较稳定。
产电模式菌的pH值为8.0，最适合反应温度为30-55度。
目前发现的柠檬酸复合酶，一般为同源二聚体或六聚体。通常分为两大类。
革兰氏阳性古细菌及真核细胞。枯草芽孢杆菌，产电模式菌和水生栖热菌，而革兰氏阳性细菌一般为同源六聚体，醋酸杆菌和亚硝单胞菌均为同源六聚体。而植物及部分真菌的过氧化物酶体，可能来源于真细菌。
由于柠檬酸复合酶，在植物代谢中，起到的重要作用，形成了稳定的复合物，这些复合物对植物，提高有机酸合成酶基因活性，增加有机酸合成与分解发挥着重要作用。
(2)、谷氨酸脱氢酶：
谷氨酸脱氢酶，普遍存在于植物体内，它虽然不是植物利用氮素的主要成份，但在植物氮代谢中，起着重要作用。
高等植物中的谷氨酸脱氢酶，主要存在于线粒体中，烟酰胺腺嘌呤二核苷酸为辅酶，它在植物的衰老过程，在高温和水份胁迫等状况下，使其铵同化的功能。但在黑暗或碳胁迫条件下，又能氧化铵，生成有机酸，从而为三羧酸循环提供了碳骨架。
在申请书中，提供的木材化学组份中，一般植物中有1％的氮；49％的氢和48％氧。
本发明是利用谷氨酸脱氢酶，在柠檬酸复合酶，在高温催化下，使其植物中1％的铵，生成有机的三羧酸，从而使三羧酸与过氧化氢酶，双氧水岐化反应。
把植物中的氢、氧、氮三种物质转化。大部份氢原子作为热源被利用，一部份氧、氢原子生成水。
从而实现能自催、自加热清洁制造纤维素的可能。这是本发明与传统方法的最大不同之处。
(3)、过氧化氢酶：
过氧化氢酶是催化过氧化氢分解成氧和水的酶。它的主要作用就是催化双氧水分解成水和氧气。
使得双氧水不至于与氧气，在铁粒子，在鳌合物作用下，反应生成对制造纤维素有害的物质，造成纤维素反黄的现象。而对纤维素没有影响，因而在漂白纤维上残留的双氧水被彻 底水解，避免纤维素进一步氧化。一般pH值为6-8，温度在20-55℃，但是与有机酸、双氧水、特效复合生物酶制剂混合使用，一般pH值为1.5-5.5，温度在80-90℃时，加快双氧水的分解，起到了关键作用，是本发明的独到之处。
两种酶分别是上海科顺生物科技，西安等公司都有售。价格在99-220元/公斤，根具需要不同，价格不一。过氧化氢酶和歧化酶及其它辅料全国有售。
本发明利用的酶制剂产品在应用方面，将具有以下优势：
1.各单酶基因都具有最适合酶活反应温度高、热稳定性好、反应速度快的特点；
2.开发的复合酶制剂，具有组分成分明确，作用位点已知，能有效防止副产物生成及不期待反应的发生；
3.复合酶制剂各酶成分比例可以按理想比例复配，最大发挥酶制剂协同促进效应，有效改善天然酶中的某些单酶制剂不足引起抑制反应；
4.高温的催化条件可明显降低反应体粘度，降低生产过程的动力消耗成本；
5.高温催化条件有效防止杂菌污染，提高产物质量，有利于后期工序的生产；
6.在某些情况下还可以提高反应底物的溶解度，减少反应体积，简化工艺操作条件；
7.开发特效复合生物酶制剂普遍具有良好的推送性，酶活半衰期长，可以明显节省酶制剂的使用量，且有利于酶制剂的贮存；
8.部分酶制剂还可以用于有机物反应催化体系。本发明研究的新型复合生物酶有着巨大的市场前景；
9.制剂无色无毒，天然有机、环保；
10.制备方便，便于贮存、运输，使用方便。
实施例2
木材特效复合生物酶制剂制浆配比如下：
谷氨酸脱氢酶3％、柠檬酸合成酶5％、过氧化氢酶2％、超氧化物歧化SOD占5％，烟酰胺3％，醋酸82％。
具体制浆方法如下：
1)将桉木原料干燥、称重、切片，待用；
2)将上述特效复合生物酶制剂和水按比例加入到上述桉木片中(特效复合生物酶制剂、桉木绝干质量、水的质量比为0.5：1：4.5)，混合均匀，于密闭条件下加热至临界温度60-80℃后，反应6-8h，即得桉木浆。
黏度测定：按GB/T 1548-2004标准，采用铜乙二胺法测定；卡伯值：按GB/T 1546-2004标准测定；
将上述的桉木浆按照60g/m 2抄造成纸张，白度测定：浆样经纤维疏解器疏解后，在布氏漏斗上抄片，经风干后，用YQ-Z-48A型白度颜色测定仪测定白度。
检测结果表明：桉木浆的卡伯值为11.5，粘度为651.2ml/g，纸浆的白度为80.3％ISO。
实施例3
木材特效复合生物酶制剂制浆配比如下：
谷氨酸脱氢酶3％、柠檬酸合成酶5％、过氧化氢酶2％、超氧化物歧化SOD占5％，烟酰胺3％，醋酸82％。
具体制浆方法如下：
1)将蔗渣原料干燥、称重，待用；
2)将上述特效复合生物酶制剂和水按比例加入到上述蔗渣中(特效复合生物酶制剂、蔗渣绝干质量、水的质量比为0.4：1：5)，混合均匀，于密闭条件下加热至临界温度60-80℃后，反应6-8h，即得蔗渣浆。
黏度测定：按GB/T 1548-2004标准，采用铜乙二胺法测定；卡伯值：按GB/T 1546-2004标准测定；
将上述的蔗渣浆按照60g/m 2抄造成纸张，白度测定：浆样经纤维疏解器疏解后，在布氏漏斗上抄片，经风干后，用YQ-Z-48A型白度颜色测定仪测定白度。
检测结果表明：按木浆的卡伯值为8.5，粘度为467.2ml/g，纸浆的白度为79.3％ISO。
最后应该说明的是，以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述实施例所记载的技术方案进行修改，或者对其中部分进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。上述虽然对本发明的具体实施方式进行了描述，但并非对本发明保护范围的限制，所属领域技术人员应该明白，在本发明的技术方案的基础上，本领域技术人员不需要付出创造性劳动即可做出的各种修改或变形仍在本发明的保护范围以内。

Claims (10)

  1. 一种特效复合生物酶制剂，其特征在于，由如下重量份的原料组成：谷氨酸脱氢酶1-5份、柠檬酸合成酶3-8份、过氧化氢酶1-4份、歧化酶占3-8份，辅助酶1-5份，有机酸营养液70-90份。
  2. 如权利要求1所述的特效复合生物酶制剂，其特征在于，所述特效复合生物酶制剂，由如下重量份的原料组成：谷氨酸脱氢酶3-5份、柠檬酸合成酶5-8份、过氧化氢酶2-4份、歧化酶占5-8份，辅助酶3-5份，有机酸营养液82-90份。
  3. 如权利要求1所述的特效复合生物酶制剂，其特征在于，所述特效复合生物酶制剂，由如下重量份的原料组成：谷氨酸脱氢酶1-3份、柠檬酸合成酶3-5份、过氧化氢酶1-2份、歧化酶占3-5份，辅助酶1-3份，有机酸营养液70-82份。
  4. 如权利要求1所述的特效复合生物酶制剂，其特征在于，由如下重量份的原料组成：谷氨酸脱氢酶3份、柠檬酸合成酶5份、过氧化氢酶2份、歧化酶占5份，辅助酶3份，有机酸营养液82份。
  5. 如权利要求1所述的特效复合生物酶制剂，其特征在于，所述的歧化酶为超氧化物歧化SOD占5％。
  6. 如权利要求1所述的特效复合生物酶制剂，其特征在于，所述辅助酶为烟酰胺。
  7. 如权利要求1所述的特效复合生物酶制剂，其特征在于，所述有机酸营养液为醋酸。
  8. 一种特效复合生物酶制剂的处理植物原料的方法，包括：
    1)将植物原料干燥、称重、切片，待用；
    2)将上述特效复合生物酶制剂和水按比例加入到上述植物原料中，混合均匀，于密闭条件下经催化缴活或外加热至临界温度60-80℃后，反应一段时间，即得所述特效复合生物酶制剂。
  9. 如权利要求8所述的方法，其特征在于，所述特效复合生物酶制剂、水和植物原料的质量比为0.6∶1：4-5。
  10. 如权利要求8所述的方法，其特征在于，所述植物原料为桉木或蔗渣。
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