WO2019165551A1 - Protéines facilitant la biosynthèse des alcaloïdes et procédés d'utilisation - Google Patents

Protéines facilitant la biosynthèse des alcaloïdes et procédés d'utilisation Download PDF

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Publication number
WO2019165551A1
WO2019165551A1 PCT/CA2019/050239 CA2019050239W WO2019165551A1 WO 2019165551 A1 WO2019165551 A1 WO 2019165551A1 CA 2019050239 W CA2019050239 W CA 2019050239W WO 2019165551 A1 WO2019165551 A1 WO 2019165551A1
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Prior art keywords
seq
benzylisoquinoline
nucleic acid
compound
substrate
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PCT/CA2019/050239
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English (en)
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Peter J. Facchini
Jillian M. HAGEL
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Serturner Corp.
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Publication of WO2019165551A1 publication Critical patent/WO2019165551A1/fr

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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/225—Tyrosine; 3,4-Dihydroxyphenylalanine
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group

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  • Life Sciences & Earth Sciences (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des procédés de conversion d'un substrat en un alcaloïde, comprenant un composé de benzylisoquinoline ou un composé précurseur de benzylisoquinoléine, dans un système de biosynthèse impliquant la croissance de cellules hôtes. Les cellules comprennent une protéine facilitant la biosynthèse des alcaloïdes et une enzyme biosynthétique complémentaire pour produire le composé alcaloïde. L'invention concerne également des compositions associées.
PCT/CA2019/050239 2018-02-28 2019-02-28 Protéines facilitant la biosynthèse des alcaloïdes et procédés d'utilisation WO2019165551A1 (fr)

Applications Claiming Priority (2)

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US201862636389P 2018-02-28 2018-02-28
US62/636,389 2018-02-28

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WO2019165551A1 true WO2019165551A1 (fr) 2019-09-06

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020198373A1 (fr) * 2019-03-26 2020-10-01 Antheia, Inc. Procédés pour améliorer la production d'alcaloïdes de type morphinane et de dérivés
CN114573508A (zh) * 2020-12-01 2022-06-03 中国科学院上海有机化学研究所 羟考酮类化合物、其中间体及制备方法
WO2022234005A1 (fr) 2021-05-07 2022-11-10 River Stone Biotech Aps Opioïdes glycosylés
US11560578B2 (en) 2017-12-05 2023-01-24 Antheia, Inc. Neopinone isomerase and methods of using

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014143744A2 (fr) * 2013-03-15 2014-09-18 The Board Of Trustees Of The Leland Stanford Junior University Microbes produisant des alcaloïdes de benzylisiquinoline (bia) et leurs procédés de fabrication et d'utilisation
WO2018005553A1 (fr) * 2016-06-27 2018-01-04 Intrexon Corporation Compositions et procédés de fabrication d'alcaloïdes de benzylisoquinoline, d'alcaloïdes de morphinane, de thébaïne et de dérivés de ceux-ci
WO2018000089A1 (fr) * 2016-06-27 2018-01-04 Serturner Corp. Polynucléotides et polypeptides utiles pour la fabrication de composés alcaloïdes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014143744A2 (fr) * 2013-03-15 2014-09-18 The Board Of Trustees Of The Leland Stanford Junior University Microbes produisant des alcaloïdes de benzylisiquinoline (bia) et leurs procédés de fabrication et d'utilisation
WO2018005553A1 (fr) * 2016-06-27 2018-01-04 Intrexon Corporation Compositions et procédés de fabrication d'alcaloïdes de benzylisoquinoline, d'alcaloïdes de morphinane, de thébaïne et de dérivés de ceux-ci
WO2018000089A1 (fr) * 2016-06-27 2018-01-04 Serturner Corp. Polynucléotides et polypeptides utiles pour la fabrication de composés alcaloïdes

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BEAUDOIN, GAW.: "Characterization of Oxidative Enzymes Involved in the Biosynthesis of Benzylisoquinoline Alkaloids in Opium Poppy (Papaver somniferum", THESIS. UNIVERSITY OF CALGARY, March 2015 (2015-03-01), Calgary, Alberta, Canada, XP055634193, Retrieved from the Internet <URL:https://prism.ucalgary.ca/bitstream/11023/2115/2/ucalgary_2015_Beaudoin_Guillaume.pdf> [retrieved on 20190326] *
DASTMALCHI, M. ET AL.: "Family portraits: the enzymes behind benzylisoquinoline alkaloid diversity", PHYTOCHEM REV., 17 June 2017 (2017-06-17), pages 249 - 277, XP036493531, [retrieved on 20190508], doi:10.1007/s11101-017-9519-z *
DATABASE Nucleotide 31 March 2008 (2008-03-31), ZULAK, KG . ET AL.: "Gene transcript and metabolite profiling of elicitor-induced opium poppy cell cultures reveals the coordinate regulation of primary and secondary metabolism", XP055634199, Database accession no. FE967356 *
FOSSATI, E. ET AL.: "Synthesis of Morphinan Alkaloids in Saccharomyces cerevisiae", PLOS ONE, vol. 10, no. 4, 23 April 2015 (2015-04-23), pages e0124459, XP055317484, [retrieved on 20190326], doi:10.1371/journal.pone.0124459 *
GALANIE, S. ET AL.: "Complete biosynthesis of opioids in yeast", SCIENCE, vol. 349, no. 6252, 4 September 2015 (2015-09-04), pages 1095 - 1100, XP055317485, [retrieved on 20190508], doi:10.1126/science.aac9373 *
NAKAGAWA, A. ET AL.: "Total biosynthesis of opiates by stepwise fermentation using a engineered Escherichia coli", NAT COMMUN, vol. 7, no. 10390, 5 February 2016 (2016-02-05), pages 1 - 8, XP055634246 *
THODEY, K. ET AL.: "A microbial biomanufacturing platform for natural and semi-synthetic opiates", NAT. CHEM. BIOL., vol. 10, no. 10, October 2014 (2014-10-01), pages 837 - 844, XP055165154 *
ZULAK, KG . ET AL.: "Gene transcript and metabolite profiling of elicitor-induced opium poppy cell cultures reveals the coordinate regulation of primary and secondary metabolism", PLANTA, vol. 225, no. 5, April 2007 (2007-04-01), pages 1085 - 1106, XP019491747, ISSN: 0032-0935 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11560578B2 (en) 2017-12-05 2023-01-24 Antheia, Inc. Neopinone isomerase and methods of using
WO2020198373A1 (fr) * 2019-03-26 2020-10-01 Antheia, Inc. Procédés pour améliorer la production d'alcaloïdes de type morphinane et de dérivés
CN114302951A (zh) * 2019-03-26 2022-04-08 安思雅公司 提高吗啡喃生物碱和衍生物的产量的方法
CN114573508A (zh) * 2020-12-01 2022-06-03 中国科学院上海有机化学研究所 羟考酮类化合物、其中间体及制备方法
WO2022234005A1 (fr) 2021-05-07 2022-11-10 River Stone Biotech Aps Opioïdes glycosylés

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