WO2019163453A1 - Dispositif de traitement - Google Patents

Dispositif de traitement Download PDF

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Publication number
WO2019163453A1
WO2019163453A1 PCT/JP2019/003209 JP2019003209W WO2019163453A1 WO 2019163453 A1 WO2019163453 A1 WO 2019163453A1 JP 2019003209 W JP2019003209 W JP 2019003209W WO 2019163453 A1 WO2019163453 A1 WO 2019163453A1
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WO
WIPO (PCT)
Prior art keywords
container
processing
liquid
air chamber
flow path
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PCT/JP2019/003209
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English (en)
Japanese (ja)
Inventor
守 西谷
隆 光安
高雄 男澤
Original Assignee
富士フイルム株式会社
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Application filed by 富士フイルム株式会社 filed Critical 富士フイルム株式会社
Priority to JP2020501624A priority Critical patent/JP6955306B2/ja
Publication of WO2019163453A1 publication Critical patent/WO2019163453A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the disclosed technology relates to a processing apparatus.
  • Japanese Patent No. 6164612 discloses a collection chamber for collecting at least one of a treatment liquid and cells, an inlet for introducing the treatment liquid, a tissue holding chamber provided above the collection chamber, and a collection
  • a cell separation container comprising a compartment filter that divides a chamber and a tissue holding chamber is described.
  • the treatment liquid introduced into the tissue holding chamber can be held in the tissue holding chamber without passing through the compartment filter, or the compartment filter Has a pressure control mechanism capable of switching between passing through and discharging to the collection chamber.
  • the atmospheric pressure adjusting mechanism includes a first atmospheric pressure adjusting unit that is provided in the collection chamber and adjusts the inflow and outflow of gas in the collection chamber, and a second atmospheric pressure adjustment unit that is provided in the tissue holding chamber and adjusts the inflow and outflow of gas in the tissue holding chamber. .
  • the living tissue is composed of cells and an extracellular matrix existing around the cells.
  • the separation process for separating (or isolating) cells from a biological tissue is a process for extracting a cell by decomposing an extracellular matrix using a treatment liquid such as an enzyme agent. In the separation process, it is important to maintain the surrounding environment of the living tissue and the cells separated from the living tissue in a sterile state in order to increase the survival rate of the cells.
  • Patent Document 1 Japanese Patent No. 6164612
  • Japanese Patent No. 6164612 Japanese Patent No. 6164612
  • a cell separation container suitable for separation treatment is disclosed.
  • the second atmospheric pressure control unit that suppresses the increase in the atmospheric pressure in the tissue holding chamber to the atmosphere.
  • contamination sources such as bacteria and viruses.
  • aseptic filters that are commercially available are widely used in order to prevent invasion of germs from the part that is open to the atmosphere.
  • the disclosed technology suppresses the increase in air pressure inside the container that accompanies the transfer of the treatment liquid to the container for housing the living tissue while suppressing the risk that the biological tissue and the cells separated from the living tissue are exposed to the contamination source
  • the purpose is to do.
  • a processing apparatus includes a first container for containing a biological tissue to be processed, at least one second container for sealing a processing liquid used for processing the biological tissue, Forming a closed system in the first container, a liquid-feeding passage for connecting the container and the second container, a processing liquid transfer means for transferring the processing liquid from the second container to the first container, and And a connected third container having a variable volume.
  • the third container having a variable volume is connected to the first container so as to form a closed system, and thus the living tissue and the cells separated from the living tissue are contaminated. It is possible to suppress an increase in the atmospheric pressure inside the first container accompanying the transfer of the processing liquid to the first container, while suppressing the risk of exposure to water.
  • the third container may include a bag made of a flexible material.
  • the flexible bag material may be, for example, a resin film. By using a resin film as the material of the bag, the manufacturing cost of the bag can be suppressed and disposable.
  • the 3rd container may be comprised including the syringe.
  • the first container includes a first air chamber and a second air chamber that are separated from each other by a partition filter, a first circulation port and a second circulation port that communicate with the first air chamber, respectively, A third circulation port communicating with the air chamber.
  • the first circulation port may be connected to the liquid supply channel
  • the second circulation port may be connected to the third container
  • the third circulation port may be connected to the drainage channel.
  • the processing apparatus may further include a drain valve provided in the drain flow path that is closed while the processing liquid is being sent from the second container to the first container.
  • a drain valve provided in the drain flow path that is closed while the processing liquid is being sent from the second container to the first container.
  • the atmospheric pressure of the first container can be adjusted by opening / closing control of the drain valve.
  • the third container is generated along with the inflow of gas into the third container while the processing liquid is being fed from the second container to the first container.
  • the volume of is increased.
  • FIG. 1 is a front view illustrating an example of a configuration of a processing apparatus 1 according to the first embodiment of the disclosed technique.
  • the processing apparatus 1 includes a processing container 20 for storing a biological tissue to be processed and a plurality of processing liquid enclosures 10 for enclosing a processing liquid used for a biological tissue separation process. Further, the processing apparatus 1 includes an individual flow path 31 and a common flow path 32 as flow paths that connect the processing container 20 and each of the plurality of processing liquid enclosures 10. Further, the processing apparatus 1 includes a liquid feed pump 51 as a processing liquid transfer unit that transfers the processing liquid from the processing liquid enclosure 10 to the processing container 20. The processing apparatus 1 decomposes the extracellular matrix constituting the biological tissue by adding the processing liquid enclosed in the processing liquid enclosure 10 to the biological tissue accommodated in the processing container 20, and removes cells from the biological tissue. The separation process to separate is performed automatically.
  • a disinfecting solution for disinfecting the living tissue for example, a disinfecting solution for disinfecting the living tissue, a washing solution for washing away the disinfecting solution, at least one enzyme solution for decomposing the living tissue, and a culture solution for washing off the enzyme solution are used as the treating solution.
  • the disinfectant solution, the cleaning solution, the enzyme solution, and the culture solution are enclosed in different treatment solution enclosures 10.
  • different types of treatment liquids are enclosed in the plurality of treatment liquid enclosures 10.
  • Different types of treatment liquids enclosed in the plurality of treatment liquid enclosures 10 are transferred to the treatment container 20 in a predetermined order. It is preferable that different types of processing liquids are not mixed in the processing liquid enclosure 10, the processing container 20, and the flow path.
  • Each of the processing liquid enclosures 10 is preferably a disposable sealed container in order to suppress the risk of contamination by external sources of contamination such as bacteria and viruses.
  • a container commercially available as a centrifuge tube is used as the processing liquid. It can be used as the enclosure 10.
  • a syringe or bag having a variable volume can be suitably used as the processing liquid enclosure 10.
  • FIG. 1 illustrates a case where a syringe is used as the treatment liquid enclosure 10.
  • Each of the plurality of individual flow paths 31 is provided corresponding to each of the plurality of treatment liquid enclosures 10.
  • Each of the individual channels 31 has one end connected to the corresponding processing liquid enclosure 10 and the other end connected to the common channel 32.
  • a joint 35 is provided at each connection portion between each individual flow path 31 and the common flow path 32.
  • the individual flow path 31 means a flow path section from the processing liquid enclosure 10 to the joint 35.
  • the common flow path 32 means a flow path section from each joint 35 to the processing container 20. Accordingly, the flow path section between the joints 35 adjacent to each other belongs to the common flow path 32.
  • the processing container 20 is connected to one end of the common flow path 32.
  • a liquid feed valve 41 is provided in the middle of each individual flow path 31. That is, each of the plurality of liquid supply valves 41 is provided corresponding to each of the plurality of processing liquid enclosures 10.
  • Each of the liquid supply valves 41 is used for selectively transferring the processing liquid from the processing liquid enclosure 10 to the processing container 20. That is, when the selected liquid feeding valve 41 is opened and the liquid feeding pump 51 is operated, the processing liquid sealed in the processing liquid enclosure 10 corresponding to the liquid feeding valve 41 is transferred to the corresponding individual flow. It is transferred to the processing container 20 via the channel 31 and the common channel 32.
  • a pinch valve can be suitably used as the liquid feeding valve 41. By using a pinch valve as the liquid feed valve 41, it is possible to open and close the flow channel without contacting the fluid passing through the individual flow channel 31.
  • the individual flow path 31 and the common flow path 32 are configured by tubular members. It is preferable that the tubular members constituting the individual flow path 31 and the common flow path 32 can be sterilized, have less eluate, and have corrosion resistance to the processing liquid. Moreover, it is preferable that the tubular member which comprises the individual flow path 31 and the common flow path 32 has thermoplasticity. Since the tubular member has thermoplasticity, the tubular member can be sealed by heat welding after use, so that, for example, when a living tissue or a cell is infected with a contamination source such as a cell and a virus, the contamination source is external. The risk of leaking out can be suppressed. As a tubular member constituting the individual flow path 31 and the common flow path 32, for example, a silicon tube can be used.
  • the joint 35 preferably has corrosion resistance to the processing liquid.
  • Teflon registered trademark
  • polypropylene or polyvinylidene fluoride
  • the joint 35 is preferably capable of heat sterilization treatment and gamma ray sterilization treatment, and polyvinylidene fluoride is suitable as a material for the joint 35.
  • the liquid feed pump 51 is provided in the middle of the common flow path 32.
  • a tube pump can be suitably used as the liquid feed pump 51.
  • the fluid can be transferred without contacting the fluid passing through the common flow path 32.
  • FIG. 2 is a diagram illustrating an example of the configuration of the processing container 20.
  • the processing container 20 has a first air chamber 21 and a second air chamber 22 which are separated from each other by a partition filter 23.
  • the processing container 20 has a first circulation port 24 and a second circulation port 25 that respectively communicate with the first air chamber 21, and a third air chamber 22 that communicates with the second air chamber 22 via a conduit 27.
  • a circulation port 26 One end of the pipe line 27 is connected to the third flow port 26, passes through the first air chamber 21 and the partition filter 23, and the other end reaches the second air chamber 22.
  • the first circulation port 24 is connected to one end of the common flow path 32. That is, the processing liquid sealed in each of the processing liquid sealing containers 10 flows into the first air chamber 21 of the processing container 20 from the first circulation port 24.
  • the first air chamber 21 is a space in which a biological tissue to be processed and cells separated from the biological tissue by separation processing are accommodated.
  • the second air chamber 22 is a space for storing used processing liquid.
  • the partition filter 23 has, for example, a filter hole having a size smaller than the size of the cells separated from the living tissue. It is possible to keep the processing liquid flowing into the processing container 20 in the first air chamber 21 by maintaining the pressure of the first air chamber 21 lower than the pressure of the second air chamber 22. It is. On the other hand, the processing liquid can be transferred from the first air chamber 21 to the second air chamber 22 by making the air pressure of the second air chamber 22 lower than the air pressure of the first air chamber 21. It is. In order to keep the processing liquid flowing into the processing container 20 in the first air chamber 21, surface tension acting on the processing liquid or force by which the partition filter 23 supports the processing liquid may be used.
  • a sealed container 90 is connected to the other end of the exhaust passage 33.
  • the sealed container 90 is connected to the processing container 20 in a closed system. “Connected in a closed system” means a state in which contamination sources such as bacteria and viruses are prevented from entering the system.
  • the volume of the sealed container 90 is variable.
  • a syringe 91 can be used as shown in FIG.
  • the syringe 91 includes a barrel 92 and a plunger 93.
  • the plunger 93 slides along the inner wall of the barrel 92, so that the volume in the barrel 92 expands or contracts.
  • an exhaust valve 42 is provided in the middle of the exhaust passage 33.
  • the exhaust valve 42 is opened when the processing liquid is transferred to the processing container 20.
  • the gas in the processing container 20 is transferred to the sealed container 90 via the exhaust passage 33, and the atmospheric pressure in the first air chamber 21 becomes atmospheric pressure.
  • the atmospheric pressure in the first air chamber 21 becomes atmospheric pressure.
  • the atmospheric pressure in the second air chamber 22 becomes higher than the atmospheric pressure. Therefore, the pressure in the first air chamber 21 is maintained lower than the pressure in the second air chamber 22.
  • One end of the drainage flow path 34 is connected to the third circulation port 26.
  • a waste liquid tank 60 is connected to the other end of the drainage flow path 34.
  • a drainage pump 52 and a drainage valve 43 are provided in the middle of the drainage flow path 34. When the drainage valve 43 is opened and the drainage pump 52 is operated, the processing liquid staying in the first gas chamber 21 is discharged from the second gas chamber 22, the third flow port 26, and the drainage. It is transferred to the waste liquid tank 60 via the liquid flow path 34.
  • a gas-liquid discrimination sensor 50 ⁇ / b> A is provided in a portion of the common flow path 32 between the liquid feed pump 51 and the processing container 20. Further, a gas-liquid discrimination sensor 50 ⁇ / b> B is provided in a portion of the drainage flow path 34 between the processing container 20 and the drainage pump 52.
  • Each of the gas-liquid discrimination sensors 50A and 50B discriminates whether the fluid flowing through the flow path is liquid or gas, and supplies a discrimination signal indicating the discrimination result to the control unit 80 (see FIG. 3) described later. To do.
  • the gas-liquid discrimination sensors 50A and 50B determine whether the fluid flowing through the flow path is liquid or gas based on, for example, the refraction angle of the laser light irradiated in the flow path. Also good.
  • FIG. 3 is a side view showing an example of the configuration of the processing apparatus 1.
  • the processing apparatus 1 includes a vibration mechanism 70 that applies vibration to the processing container 20.
  • the vibration mechanism 70 is connected to the motor 71, the cam 73 connected to the rotation shaft 72 of the motor 71, the cam follower 74 disposed so as to contact the cam 73, and the cam follower 74.
  • a vibration stage 75 that performs a linear reciprocating motion is included.
  • the drive control of the motor 71 is performed by the control unit 80.
  • a holding unit 76 that holds the processing container 20 is mounted on the vibration stage 75.
  • the vibration stage 75 performs a linear reciprocating motion, whereby vibration is applied to the processing container 20 held by the holding unit 76.
  • the control unit 80, the motor 71, the cam 73, and the cam follower 74 are accommodated in the housing 81.
  • a heater 77 and a temperature sensor 78 are embedded in the holding unit 76.
  • the holding unit 76 is made of a material having a relatively high thermal conductivity such as metal, and also functions as a heat block that transfers heat generated from the heater 77 to the processing container 20.
  • the temperature sensor 78 includes, for example, a thermocouple, detects the temperature of the holding unit 76, and supplies a temperature detection signal indicating the detected temperature to the control unit 80.
  • the controller 80 controls the heater 77 based on the temperature detection signal from the temperature sensor 78.
  • the control unit 80 controls the heater 77 so that the temperature of the holding unit 76 is maintained at a predetermined temperature (for example, 37 ° C.).
  • the ambient temperature of the processing container 20 is kept constant (for example, 37 ° C.).
  • the control unit 80 controls the opening and closing of the liquid supply valve 41, the exhaust valve 42 and the drainage valve 43, and the drive control of the liquid feed pump 51 and the drainage pump 52 in addition to the control of the motor 71 and the heater 77.
  • FIG. 4A shows a plurality of processing liquid enclosures 10, processing containers 20, sealed containers 90, and waste liquid tanks 60 with flow paths (individual flow paths 31, common flow paths 32, exhaust flow paths 33, and drainage flow paths 34). It is a figure which shows an example of a structure of the distribution system 2 of the processing apparatus 1 comprised by connecting via.
  • FIG. 4B is a perspective view illustrating an example of the configuration of the main body 3 of the processing apparatus 1. As shown in FIG. 4A, the distribution system 2 can be attached to and detached from the main body 3 while the plurality of processing liquid enclosures 10, the processing containers 20, the sealed containers 90, and the waste liquid tank 60 are connected via the flow paths. It is.
  • the distribution system 2 can be attached to the main body 3 as it is.
  • the risk that each component of the distribution system 2 is exposed to the contamination source can be suppressed.
  • FIG. 5 is a flowchart illustrating an example of a flow of processing executed by the control unit 80 when the processing device 1 performs separation processing for separating cells from biological tissue.
  • different types of treatment liquids are enclosed in each of the plurality of treatment liquid enclosures 10.
  • biological cells to be processed are accommodated in the first air chamber 21 of the processing container 20.
  • the airtight container 90 is in a state where the plunger 93 is pushed so that the volume thereof is minimized.
  • Each of the plurality of liquid supply valves 41 is identified by an identification number N (N is a natural number) in the control unit 80. In the initial state, it is assumed that the liquid supply valve 41, the exhaust valve 42, and the drainage valve 43 are closed, and the liquid supply pump 51 and the drainage pump 52 are stopped.
  • step S1 the control unit 80 sets the set value of the identification number N of the liquid delivery valve 41 to 1. Thereby, the liquid feeding valve 41 corresponding to the identification number 1 is selected.
  • step S2 the control unit 80 controls the exhaust valve 42 to an open state.
  • step S3 the control unit 80 controls the liquid supply valve 41 corresponding to the identification number 1 to be in an open state.
  • step S ⁇ b> 4 the control unit 80 starts the operation of the liquid feed pump 51. Thereby, the processing liquid sealed in the processing liquid sealing container 10 corresponding to the identification number 1 is transferred to the processing container 20 via the corresponding individual flow path 31 and the common flow path 32.
  • the exhaust valve 42 is opened and the drain valve 43 is closed, so that the first air chamber 21 is brought along with the inflow of the processing liquid into the processing container 20.
  • the gas inside is transferred to the sealed container 90 (syringe 91) via the exhaust flow path 33 while expanding the volume of the sealed container 90 (syringe 91).
  • an increase in the pressure of the first air chamber 21 accompanying the transfer of the processing liquid to the processing container 20 is suppressed, and the pressure of the first air chamber 21 is lower than the pressure of the second air chamber 22.
  • the processing liquid that has flowed into the processing container 20 can be retained in the first air chamber 21.
  • a biological tissue can be immersed in a process liquid.
  • step S4A the control unit 80 detects the liquid feeding state of the processing liquid by monitoring the discrimination signal supplied from the gas-liquid discrimination sensor 50A.
  • step S ⁇ b> 5 the control unit 80 determines whether or not the processing liquid has been fed.
  • the control unit 80 determines that the fluid flowing through the common flow path 32 has changed from liquid to gas based on the determination signal supplied from the gas-liquid determination sensor 50A. To do. If the controller 80 determines that the processing liquid has been supplied, the process proceeds to step S6.
  • step S6 the control unit 80 controls the liquid feeding valve 41 corresponding to the identification number 1 to be closed.
  • step S ⁇ b> 7 the control unit 80 stops the operation of the liquid feed pump 51.
  • step S8 the control unit 80 controls the exhaust valve 42 to be closed. At this time, the state in which the biological tissue and the processing liquid are accommodated in the first air chamber 21 is maintained.
  • step S ⁇ b> 9 the control unit 80 starts the operation of the vibration mechanism 70 by starting driving of the motor 71. Thereby, the processing liquid accommodated in the processing container 20 is stirred, and the process with respect to the biological tissue by the said processing liquid is accelerated
  • step S10 the control unit 80 determines whether or not a predetermined time has elapsed since the operation of the vibration mechanism 70 was started. If it is determined that the predetermined time has elapsed, the process proceeds to step S11. In step S ⁇ b> 11, the control unit 80 stops the vibration mechanism 70 by stopping the driving of the motor 71. Thereby, the stirring process of the process liquid accommodated in the process container 20 is complete
  • step S12 the control unit 80 controls the exhaust valve 42 to be in an open state.
  • step S13 the control unit 80 controls the drain valve 43 to be in an open state.
  • step S ⁇ b> 14 the control unit 80 starts the operation of the drainage pump 52. Due to the processing from step S12 to step S14, the pressure in the second air chamber 22 becomes lower than the pressure in the first air chamber 21, and the processing liquid staying in the first air chamber 21 passes through the partition filter. It passes through and is transferred to the second air chamber 22. Thereafter, the processing liquid is transferred to the waste liquid tank 60 via the drainage flow path 34.
  • step S14A the control unit 80 monitors the determination signal supplied from the gas-liquid determination sensor 50B to detect the processing liquid supply state.
  • step S ⁇ b> 15 the control unit 80 determines whether or not the supply of the processing liquid from the processing container 20 to the waste liquid tank 60 has been completed.
  • the control unit 80 determines that the fluid flowing through the drainage flow path 34 has changed from liquid to gas based on the determination signal supplied from the gas-liquid determination sensor 50B, the processing liquid has been supplied. Is determined. If the controller 80 determines that the processing liquid has been supplied, the process proceeds to step S16.
  • step S16 the control unit 80 controls the drain valve 43 to be closed.
  • step S ⁇ b> 17 the control unit 80 stops the operation of the drainage pump 52.
  • step S18 the control unit 80 determines whether or not the processing from step S2 to step S17 has been completed for all the processing liquids enclosed in each of the processing liquid enclosures 10.
  • control unit 80 performs the above determination by determining whether or not the set value of the current identification number N of the liquid supply valve 41 is the maximum value corresponding to the number of processing liquid enclosures 10. Also good. When it is determined that the processing from step S2 to step S17 has been completed for all the processing liquids, the control unit 80 ends this routine. On the other hand, if it is determined that the processing from step S2 to step S17 is not completed for all the processing liquids, the control unit 80 shifts the processing to step S19.
  • step S19 the control unit 80 increases the set value of the identification number N of the liquid delivery valve 41 by one, and returns the process to step S2.
  • the liquid supply valve 41 corresponding to the identification number 2 is selected, and the processing from step S2 to step S17 is performed on the processing liquid sealed in the processing liquid enclosure 10 corresponding to the identification number 2.
  • the processing from step S2 to step S17 is repeated until the processing is completed for all the processing liquids sealed in each of the processing liquid enclosures 10.
  • the processing liquids of different types are sequentially transferred from the processing liquid sealing container 10 to the processing container 20 and used.
  • This processing liquid can be automatically transferred from the processing container 20 to the waste liquid tank 60.
  • the first air chamber 21 of the processing container 20 is connected to a sealed container 90 having a variable volume, when the processing liquid is transferred to the processing container 20, the gas in the first air chamber 21 is sealed.
  • the container 90 is transferred to the sealed container 90 while expanding the volume of the container 90.
  • an increase in the atmospheric pressure of the first air chamber 21 accompanying the transfer of the processing liquid to the processing container 20 can be suppressed, and the atmospheric pressure of the first air chamber 21 is higher than the atmospheric pressure of the second air chamber 22. It is possible to maintain a low state.
  • the processing liquid that has flowed into the processing container 20 can be retained in the first air chamber 21. Since the sealed container 90 is connected to the processing container 20 in a closed system, it is possible to suppress the risk that the living tissue and the cells separated from the living tissue are exposed to the contamination source.
  • the minimum pore diameters of the sterile filters manufactured by Sartorius and Millipore are 0.2 ⁇ m and 0.1 ⁇ m (both are catalog values), respectively.
  • the size of the virus is 0.1 ⁇ m or less. It is shown that.
  • bacteria and fungi it states that it can be removed, but not all viruses and mycoplasmas can be removed.
  • contamination sources such as viruses and mycoplasma that are smaller than the size of the filter hole of the sterile filter can pass through the sterile filter. Therefore, according to an aspect in which a sterile filter is connected to the end of the exhaust flow path 33 instead of the sealed container 90, there is a risk that the biological tissue and cells separated from the biological tissue are contaminated.
  • the closed container 90 that is connected to the processing container 20 by forming a closed system is used as the air pressure adjusting means of the first air chamber 21. Invasion of contamination sources such as viruses and mycoplasmas that cannot be protected even by a sterile filter can be suppressed.
  • FIG. 6 is a front view illustrating an example of a configuration of a processing apparatus 1A according to the second embodiment of the disclosed technique.
  • the processing apparatus 1 ⁇ / b> A includes a bag 94 as a sealed container 90 connected to one end of the exhaust flow path 33 and having a variable volume. That is, the bag 94 is used as an alternative to the syringe 91 (see FIG. 1) in the processing apparatus 1 according to the first embodiment.
  • the bag 94 is connected to the processing container 20 in a closed system.
  • the bag 94 includes, for example, a flexible resin film.
  • the exhaust valve 42 is opened and the drain valve 43 is closed, as in the processing apparatus 1 (see FIG. 1) according to the first embodiment.
  • the gas in the first air chamber 21 is transferred to the sealed container 90 (bag 94) while expanding the volume of the sealed container 90 (bag 94).
  • an increase in the pressure of the first air chamber 21 accompanying the transfer of the processing liquid to the processing container 20 is suppressed, and the pressure of the first air chamber 21 is maintained at the atmospheric pressure.
  • the atmospheric pressure in the second air chamber 22 becomes higher than the atmospheric pressure. Therefore, the air pressure in the first air chamber 21 can be kept lower than the air pressure in the second air chamber 22. As a result, the processing liquid that has flowed into the processing container 20 can be retained in the first air chamber 21. Thereby, in the 1st air chamber 21, a biological tissue can be immersed in a process liquid.
  • the syringe 91 when used as the sealed container 90, due to the sliding resistance of the plunger 93 that slides along the inner wall of the barrel 92, along with the transfer of the processing liquid to the processing container 20, The air pressure in the first air chamber 21 slightly increases, and as a result, the processing liquid may leak into the second air chamber 22.
  • the bag 94 since the bag 94 is used as the sealed container 90, there is almost no drag such as sliding resistance against the expansion of the volume, so that the pressure of the first air chamber 21 accompanying the transfer of the processing liquid to the processing container 20 Can be sufficiently suppressed, and the risk of the processing liquid leaking into the second air chamber 22 can be reduced.
  • the aspect which performs the transfer of the process liquid from each of the process liquid enclosure 10 to the process container 20 with the liquid feed pump 51 provided in the middle of the common flow path 32 is illustrated.
  • the treatment liquid is transferred from each of the treatment liquid enclosures 10 to the treatment container 20 to each of the plurality of treatment liquid enclosures 10. You may carry out by the syringe pump provided accompanying.
  • processing container 20 is an example of a first container in the disclosed technology.
  • the treatment liquid enclosure 10 is an example of a second container in the disclosed technology.
  • the sealed container 90 is an example of a third container in the disclosed technology.
  • the liquid feed pump 51 is an example of a processing liquid transfer unit in the disclosed technology.

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
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Abstract

Ce dispositif de traitement comprend un premier récipient pour stocker un tissu biologique à traiter, au moins un second récipient pour sceller un liquide de traitement à utiliser dans le traitement du tissu biologique, un trajet d'écoulement qui relie le premier récipient et le second récipient, un moyen de transfert de liquide de traitement pour transférer le liquide de traitement du second récipient au premier récipient, et un troisième récipient à capacité variable relié formant un système fermé dans le premier récipient.
PCT/JP2019/003209 2018-02-23 2019-01-30 Dispositif de traitement WO2019163453A1 (fr)

Priority Applications (1)

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JP2020501624A JP6955306B2 (ja) 2018-02-23 2019-01-30 処理装置

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JP2018030807 2018-02-23
JP2018-030807 2018-02-23

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WO2019163453A1 true WO2019163453A1 (fr) 2019-08-29

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Citations (5)

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WO2012017663A1 (fr) * 2010-08-04 2012-02-09 財団法人ヒューマンサイエンス振興財団 Colonne de séparation de cellules mononucléaires, système de séparation de cellules mononucléaires, procédé de séparation de cellules mononucléaires, cellules mononucléaires, et médicament destiné à une administration interne
WO2012122603A1 (fr) * 2011-03-15 2012-09-20 Regeneus Pty Ltd Méthode et dispositif de traitement cellulaire
WO2013103108A1 (fr) * 2012-01-04 2013-07-11 株式会社ジャパン・ティッシュ・エンジニアリング Récipient pour séparer des cellules
WO2014072756A1 (fr) * 2012-11-06 2014-05-15 Utb Envirotec Zrt. Procédé d'hydrolyse et d'acidification de déchets organiques et appareil correspondant
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