WO2019127625A1 - Method for predicting assimilation product yields of greenhouse solanaceae type crops - Google Patents

Abstract

A method for predicting assimilation product yields of greenhouse solanaceae type crops, the method comprising the following steps: 1) establishing a prediction model for simple-leaf assimilation product yields of greenhouse solanaceae type crops; 2) determining an effective leaf area of a greenhouse solanaceae type crop; and 3) substituting the obtained effective leaf area of the greenhouse solanaceae type crop and a light interception amount into the prediction model in the step 1 so as to obtain an assimilation product yield of the greenhouse solanaceae type crop. According to the method, the defects in predicting assimilation product yields of solanaceae type crops are overcome, a theoretical basis is provided for improving prediction of greenhouse solanaceae type crop yield, and a certain advancing effect is provided for improving the level of technology for greenhouse solanaceae type crop cultivation.

Description

Method for predicting yield of assimilation products of greenhouse solanaceous crops Technical field

The invention belongs to the technical field of facility cultivation, and particularly relates to a method for predicting the yield of assimilation products of greenhouse solanaceous crops.

Background technique

Solanum crops are one of the main crops in greenhouse cultivation, and the greenhouse sugarhouse crop growth simulation model plays a crucial role in greenhouse crop cultivation management.

In the study of growth and development simulation of greenhouse solanaceous crops, the yield of assimilation products is an important link in the simulation model of greenhouse growth and development. There are two methods for calculating the yield of assimilated products. The first is to use destructive sampling to obtain the growth of the dry weight of the solanaceous fruit, and use it as the greenhouse solanaceous crop yield. The second method uses the photosynthesis model. The output of the model is used as the yield of the assimilation product.

Both methods have the following disadvantages: First, the first method calculates the yield of assimilation products as the net assimilation product yield. After photosynthesis, the assimilated products are transported to various organs through the transport organization, and each organ receives the assimilation product. After maintaining respiratory consumption, the remaining part of the assimilation product can be converted into a component of each organ. Therefore, the yield of assimilated products obtained by the first method is lower than that of the actual assimilated products. The yield of assimilated products calculated by the second method is high. The reason is that when photosynthesis of greenhouse solanaceous crop leaves is used, assimilation needs to maintain breathing to maintain the life activities of the leaves, and consumes a part of assimilation products, so the second type is adopted. The yield value of the assimilation product obtained by the method is higher than the actual assimilation product amount.

It is well known that the greenhouse solanaceous crops are transported to various organs in the form of sucrose after photosynthesis by leaves, so the amount of sucrose passing through the petiole during the day is the assimilation product of the leaf for one day; the leaves of the whole plant of the solanaceous fruit are passed through the petiole. The amount of sucrose is added, which is the assimilation product yield of greenhouse solanaceous crops in a day.

In view of this, the present invention devises a method for predicting the yield of assimilation products of greenhouse solanaceous crops. For greenhouse solanaceous crops, the leaf output is sucrose, and the change of the concentration of sucrose in the petiole during the day is determined. The production of sucrose in the leaves in one day finally yielded the assimilation product yield of greenhouse solanaceous crops.

Summary of the invention

To overcome the deficiencies in the prior art described above, the present invention provides a method for predicting the yield of assimilation products of greenhouse solanaceous crops.

The invention is achieved by the following technical solutions:

A method for predicting the yield of assimilation products of greenhouse solanaceous crops, comprising the following steps:

Step 1: Establish a single leaf assimilation product prediction model for greenhouse solanaceous crops: determine the single leaf sucrose yield of greenhouse solanaceous crops, analyze the corresponding relationship between single leaf sucrose yield and light interception and establish an equation;

Step 2: Determine the effective leaf area of the greenhouse solanaceous crop: firstly determine the leaf area Li of the crop according to the effective accumulated temperature GDD, and then determine the effective leaf area LA of the crop by using the effective leaf area and the leaf area conversion coefficient;

Step 3: Substituting the determined effective leaf area LA and light interception amount I into the prediction model in step 1, the assimilation product yield Y _{1 of the} greenhouse solanaceous crop is obtained.

Further, the step 1 determines the single leaf sucrose yield of the greenhouse solanaceous crops by cutting a 1 mm deep knife edge with a blade, and then collecting the phloem juice with a capillary tube. After constant volume, the solution is determined by high performance liquid chromatography. Sucrose concentration.

Further, the effective leaf area determination in the step 2 is to determine the effective leaf area conversion coefficient of different growth stages by experiment: the ratio of the light intensity b of the light receiving blade below the light saturation point to the light saturation point a, that is, light interception conversion coefficient

Multiply the leaf area by the light interception conversion factor

The converted area is called the single leaf effective leaf area, and the effective leaf area of the whole plant is obtained by accumulating the effective leaf area of the whole plant. The ratio of the effective leaf area to the leaf area of the whole plant is the effective leaf area conversion coefficient. The effective leaf area conversion coefficient can be used to calculate the effective leaf area LA of a single crop on any day.

Further, the assimilation product yield of the greenhouse solanaceous crop

Yz is the maximum sucrose yield of the leaves under optimal water and fertilizer supply conditions, a is the model parameter, and I is the leaf light interception.

The invention has the beneficial effects of:

The present invention establishes a model for predicting the sucrose yield of greenhouse solanaceous crops by using the amount of sucrose as the assimilation product yield. Compared with the previous studies, it overcomes the low prediction results caused by the dry weight of each organ as the assimilation product and the high yield of assimilated products output by the traditional photosynthesis model. The assimilation product yield prediction model established by this method can not only accurately predict the assimilation product yield of greenhouse solanaceous crops, but also conform to the growth law of greenhouse solanaceous crops.

DRAWINGS

Figure 1 is a graph of the light saturation point of greenhouse tomato leaves.

Detailed ways

The present invention is further described below in conjunction with specific embodiments, but the scope of protection of the present invention is not limited thereto.

A method for predicting the yield of assimilation products of greenhouse solanaceous crops takes greenhouse tomato as an example, and follows the steps below:

Step 1: Establishment of a prediction model for single leaf assimilation product yield in greenhouse tomato

(1) Determination of yield of single leaf assimilation product in greenhouse tomato

Select the middle leaves of the greenhouse tomato, cut a 1 mm deep knife edge on the phloem of the petiole, then collect the phloem juice with a capillary tube, connect the glass bottle under the capillary, and let the collected juice flow into the glass bottle to collect the petiole of the day. The phloem sap, after constant volume, the sucrose concentration in the phloem sap was determined by high performance liquid chromatography, and the sucrose yield of individual leaves in one day was obtained, which was used as the assimilation product yield of a leaf of a greenhouse tomato.

The amount of light intercepted above the blade was measured using a radiometer, and the amount of light intercepted per minute of the blade was recorded.

(2) Analyze the relationship between single leaf sucrose yield and light interception (Table 1), use the slidewrite plus software to fit the equation (formula (1)), and determine the parameters in the equation.

Table 1 Correspondence between light interception and sucrose yield

Light interception		Sucrose production
2000	6
1500	5.99
1000	5.98
800	5.9
600	5.74
400	5.27
200	3.94
100	2.5
50	1.5
0	0

Among them, Y is the leaf sucrose yield, Yz is the maximum sucrose yield of the leaves under the optimal water and fertilizer supply conditions, a is the model parameter, I is the leaf light interception; the values of Yz and a obtained by the test data of Table 1 are respectively 6 and 0.032.

Formula (1) becomes:

Step 2: Determination of effective leaf area LA of greenhouse tomato

(1) Determination of the yield of single plant assortment in greenhouse tomato

Select any tomato plant that grows normally in the greenhouse, cut a 1 mm deep knife edge on all the leaves of the tomato plant, then collect the phloem juice with a capillary tube, connect the glass bottle under the capillary tube, and let the collected juice flow into the glass bottle. The phloem sap of the petiole in one day was collected, and the sucrose concentration and volume in the phloem sap were measured to obtain the assimilation product yield of the whole plant in one day.

(2) Simulation of single plant assimilation yield in greenhouse tomato

Determination of effective leaf area of single tomato in greenhouse

When photosynthesis of greenhouse tomatoes, not all the leaves are in the optimal light receiving state, the concept of effective leaf area is proposed here; the ratio of the light intensity b of the light receiving blade below the light saturation point to the light saturation point a, ie Light interception conversion factor

Multiply the leaf area by the light interception conversion factor

The converted area is called the single leaf effective leaf area, and the effective leaf area of the whole plant is obtained by accumulating the effective leaf area of the whole plant; the ratio of the effective leaf area to the leaf area of the whole plant is the effective leaf area conversion coefficient. .

Determination of effective leaf area conversion factor:

Experimental design: Greenhouse tomato was used as research object, and perlite cultivation was carried out, and nutrient solution was poured. Representative greenhouse tomato plants were selected and tested at seedling, flowering, fruiting and harvesting stages. Measurement items: crop indicators: leaf area of each leaf position (leaf area = leaf length ² × 0.7); light interception of leaves (measured by radiometer); light saturation point (using Li-6400 photosynthetic system analyzer); environment Indicator: Temperature (greenhouse control system records itself).

Determination of the light saturation point of greenhouse tomatoes:

Using Li-6400 photosynthetic system analyzer, the photosynthesis rate of middle leaves under different light intensities was determined, and the light saturation point of greenhouse tomato leaves was determined. From Fig. 1, the light saturation point of greenhouse tomato was 2500 μmolm ^-2 s ^-1 :

Obtaining the light interception conversion coefficient in Table 2: the light interception amount of the first and second leaf positions (the light intensity of the light receiving blade below the light saturation point) based on the light interception amount (light saturation point) of the third leaf position The ratio of the light interception amount to the third leaf position is the light interception conversion coefficient; the leaf area conversion coefficient is the ratio of the sum of the effective leaf areas to the sum of the leaf areas. The light interception conversion coefficient of other growth periods (based on the last leaf position) and the leaf area conversion coefficient are obtained in the same manner as the seedling stage, as shown in Tables 3, 4 and 5.

data analysis:

Table 2 Effective leaf area conversion coefficient of greenhouse tomato in seedling stage

Table 3 Effective leaf area conversion coefficient of greenhouse tomato in flowering stage

Table 4 Effective leaf area conversion coefficient of greenhouse tomato in the fruiting stage

Table 5 Effective Leaf Area Conversion Coefficient of Greenhouse Tomatoes in Harvest Period

Table 6 Leaf area conversion coefficient of greenhouse tomato

2 Simulation of effective leaf area per plant

First calculate the effective accumulated temperature of the greenhouse tomato, using the following formula:

GDD(i)=GDD(i-1)+D(i) (3)

In the formula, GDD(i) is the total effective accumulated temperature on the i-day, GDD(i-1) is the total effective accumulated temperature on the i-1th day, and D(i) is the effective accumulated temperature on the day of the i-th day;

D(i)=T-Tb (4)

In the formula, T is the daily average temperature of the i-day, and Tb is the limit temperature, which is usually 13 ° C;

Then, the change of leaf length of greenhouse tomato was measured every day, and the leaf area per plant was calculated by the following formula:

Li=∑(Ln) ² ×0.7 (5)

In the formula, Li is the leaf area per plant, cm ² ; Ln is the leaf length of the nth leaf, cm.

Taking the effective accumulated temperature as the independent variable and using the leaf area as the dependent variable, the slidewrite plus software was used to fit the equation, and the leaf area per plant of greenhouse tomato could be predicted any day.

The equations fitted are as follows:

Step 3: Calculation of yield of assimilated product per plant

First, calculate the effective accumulated temperature of greenhouse tomato on any day according to formulas (3) and (4), and then calculate the effective leaf area LA of this day by formula (6) and effective leaf area conversion coefficient, and finally the effective leaf area LA and The measured light interception amount I is substituted into the formula (7) to obtain the total assimilation product yield.

The above description of the present invention is briefly described, and is not limited to the above-mentioned working range limits, as long as the invention and the working method are used for simple modification and application to other devices, or improvement and retouching without changing the main concept principle of the present invention. The behaviors are all within the scope of the present invention.

Claims (6)

1. A method for predicting the yield of assimilation products of greenhouse solanaceous crops, characterized in that it comprises the following steps:

   Step 1: Establish a single leaf assimilation product prediction model for greenhouse solanaceous crops: determine the single leaf sucrose yield of greenhouse solanaceous crops, analyze the corresponding relationship between single leaf sucrose yield and light interception and establish an equation;

   Step 2: Determine the effective leaf area LA of the greenhouse solanaceous crops;

   Step 3: Substituting the determined effective leaf area LA and the measured light interception amount I into the prediction model in step 1, the assimilation product yield Y _{1 of the} greenhouse solanaceous crop is obtained.
2. The method for predicting the yield of assimilation products of greenhouse solanaceous crops according to claim 1, wherein the step 1 determines the single leaf sucrose yield of the greenhouse solanaceous crops by cutting a 1 mm deep with a blade. The knife edge was then used to collect the phloem sap using a capillary tube. After constant volume, the sucrose concentration in the solution was determined by high performance liquid chromatography.
3. The method for predicting the yield of assimilation products of greenhouse solanaceous crops according to claim 1, wherein the effective leaf area determination in the step 2 is to determine the effective leaf area conversion coefficient at different growth stages by experiment: The ratio of the light intensity b of the light-receiving blade below the light saturation point to the light saturation point a, that is, the light interception conversion coefficient

   

   Multiply the leaf area by the light interception conversion factor

   

   The converted area is called the single leaf effective leaf area, and the effective leaf area of the whole plant is obtained by accumulating the effective leaf area of the whole plant. The ratio of the effective leaf area to the leaf area of the whole plant is the effective leaf area conversion coefficient. The effective leaf area conversion coefficient can be used to calculate the effective leaf area LA of a single crop on any day.
4. A method for predicting the yield of assimilating products of greenhouse solanaceous crops according to claim 3, wherein said different growth stages include seedling stage, flowering stage, fruiting stage and harvesting period.
5. A method for predicting the yield of assimilation products of greenhouse solanaceous crops according to claim 1, characterized in that the assimilation product yield of the greenhouse solanaceous crops

   

   Yz is the maximum sucrose yield of the leaves under optimal water and fertilizer supply conditions, a is the model parameter, and I is the leaf light interception.
6. A method for predicting the yield of assimilation products of greenhouse solanaceous crops according to claim 1, wherein said light interception amount is measured by a radiometer.

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