WO2019119683A1 - Plateau de stockage à base magnétique, porte-pipette, porte-seringue et plateau de stérilisation pour banc stérile - Google Patents

Plateau de stockage à base magnétique, porte-pipette, porte-seringue et plateau de stérilisation pour banc stérile Download PDF

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Publication number
WO2019119683A1
WO2019119683A1 PCT/CN2018/082743 CN2018082743W WO2019119683A1 WO 2019119683 A1 WO2019119683 A1 WO 2019119683A1 CN 2018082743 W CN2018082743 W CN 2018082743W WO 2019119683 A1 WO2019119683 A1 WO 2019119683A1
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WO
WIPO (PCT)
Prior art keywords
pipette
tray
column
horizontal
magnetic
Prior art date
Application number
PCT/CN2018/082743
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English (en)
Chinese (zh)
Inventor
刘展
彭聿平
苏建友
邱一华
方小霞
Original Assignee
南通大学
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Filing date
Publication date
Application filed by 南通大学 filed Critical 南通大学
Priority to PCT/CN2018/082743 priority Critical patent/WO2019119683A1/fr
Publication of WO2019119683A1 publication Critical patent/WO2019119683A1/fr
Priority to ZA2019/06016A priority patent/ZA201906016B/en

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26Accessories or devices or components used for biocidal treatment
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00Supporting devices; Holding devices

Definitions

  • the invention relates to a magnetic base storage tray, a pipette, a syringe holder and a sterilization tray for a clean bench.
  • test solution is quantitatively transferred between test tubes, reagent bottles, flasks, beakers, measuring cylinders, measuring cups, measuring flasks, and flasks.
  • the experimental solution of different functions can be transferred by simply changing the tip; by using the syringe and the microfilter, a small amount of the solution can be filtered and sterilized.
  • a syringe due to the scale on the syringe barrel
  • the use of a syringe allows for the quantitative transfer of the test solution between the test tube, reagent bottle, flask, beaker, graduated cylinder, measuring cup, measuring flask and conical flask; using the culture plate as a container,
  • the cells are subjected to experimental operations such as incubation and cultivation.
  • Pipettes are used as tools for quantitative transfer of solutions in scientific research experiments. The frequency of use is very high. Sometimes pipettes with a tip are used repeatedly when drawing the same test solution. Since the liquid level of the clean bench occupied by the pipette is too large, and the liquid pipette has been sucked, the liquid remaining in the suction head may flow to the pipette and the tip connection portion. Causes contamination of the pipette. This requires people to prepare a vertically placed pipette holder for the pipette in the clean bench. To ensure the stability of the pipette holder after placing the pipette, the pipette holder generally has a larger base area.
  • Syringes are commonly used as tools in scientific research experiments. In the experiment, a syringe was used multiple times in the clean bench because it was used multiple times when transferring the same experimental solution. The horizontal placement of the syringe on the level of the clean bench will occupy the space of the clean bench, and may also be contaminated by contact of the injector head with other laboratory instruments.
  • culture plates In cell culture experiments, culture plates, especially secondary culture plates, must be aseptically processed. Since the culture plate cannot be autoclaved, the general laboratory does not have the conditions for radiation sterilization. Therefore, it is common to sterilize the culture plate by ultraviolet light irradiation in a clean bench under aseptic conditions. In order to ensure the effect of UV light irradiation, the distance between the UV lamp and the culture plate should be controlled within 30 cm. Therefore, it is necessary to find some objects in the clean bench to raise the culture plate to be close to the light source of the ultraviolet lamp.
  • the object of the present invention is to provide a magnetic base storage tray, a pipette, a syringe holder and a sterilization tray for a clean platform with reasonable structure, convenient use and adjustable space.
  • the utility model relates to a magnetic base storage tray, a pipettor, a syringe rack and a sterilization tray, which are characterized in that: the clean platform comprises a magnetic seat for generating an adsorption magnetic force, and the side of the magnetic seat is a magnetic base.
  • Horizontal member height positioning lock, four horizontal member height positioning locks respectively are arranged on the column, the storage tray horizontal member can be horizontally rotated on the column, the pipette holder horizontal member, the syringe holder horizontal member, the sterilization tray member ;
  • the storage tray horizontal member includes a connecting sleeve for fitting the storage tray horizontal member on the column of the magnetic base, and assisting the storage tray horizontal member to achieve a large angular movement in the horizontal direction, and the connecting sleeve passes the horizontal arm
  • the storage tray is connected to the storage tray, and the storage tray is provided with a frame 1cm higher than the surface of the storage board for preventing the falling of the storage object.
  • the storage tray is used for accommodating the insertion or removal of the storage tray according to the experiment. Use small equipment;
  • the pipette holder horizontal member includes a pipette connection sleeve, and the pipette connection sleeve is used to sleeve the pipette holder horizontal member on the column of the magnetic base, and helps the horizontal level of the pipette holder to be horizontal
  • the pipette connection sleeve is connected to the pipette holder plate by the pipette horizontal arm, and the pipette holder plate is provided with an open pipette lower hanging groove, and the pipetting can be placed vertically downward.
  • the syringe holder horizontal member includes a syringe holder connecting sleeve for fitting the syringe holder horizontal member on the column of the magnetic base, and assisting the syringe holder horizontal member to achieve a large angular movement in the horizontal direction, the syringe holder
  • the connecting sleeve is connected to the syringe rack plate through the horizontal arm of the syringe holder, and the syringe rack plate is provided with a horizontal slot for the syringe to horizontally place the syringe;
  • the sterilizing tray horizontal member comprises a sterilizing tray connecting sleeve, and the sterilizing tray connecting sleeve is used for tying the sterilizing tray horizontal member on the column of the magnetic base, and helping the sterilizing tray horizontal member to achieve a horizontal direction The angle is moved, and the sterilizing tray connecting sleeve is connected with the horizontal placing plate of the culture plate of the cell culture plate on which the plastic material is placed through the horizontal arm of the sterilizing tray, and the anti-skid frame is arranged around the horizontally placed plate of the culture plate to prevent the plate from slipping off;
  • the storage tray horizontal member is a toughened flame-retardant reinforced polybutylene terephthalate composite composed of the following weight percentage components:
  • Toughener is 1.5 to 10% of LLDPE
  • the bromide is one or both of decabromodiphenylethane and a brominated epoxy having a molecular weight of 10,000 to 50,000.
  • the horizontal member height positioning lock comprises a positioning lock body for forming a positioning cylindrical space of the positioning lock and the magnetic base column; the positioning lock body is composed of two left and right pieces, and the left and right two pieces are connected by a positioning lock hinge.
  • the cohesive cylindrical space of the positioning lock can be effectively opened and closed; the left and right open ends of the positioning lock body are provided with positioning locking screws, and the positioning locking screw is tightened to make the positioning lock tightly fit on the column of the magnetic base. Loosen the positioning lock fastening screw, remove the positioning lock to the magnetic base column, and move the positioning lock up and down.
  • a positioning lock silicone pad is arranged in the positioning lock body, and the positioning lock silicone pad and the magnetic base column are engaged, so that the positioning lock is fixed at a height position required by the magnetic base column.
  • the magnetic base comprises a soft magnetic material, wherein the soft magnetic material is provided with a non-magnetic material that separates the soft magnetic material into uniform upper and lower halves, and a constant magnet and a constant magnet are arranged in the cylindrical space surrounded by the soft magnetic material.
  • the N and S poles of the constant magnet are in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials separated by the non-magnetic material, magnetic
  • the adsorption surface of the seat is not magnetic and can move freely; when the constant magnet rotates the switch to the open position, the N and S poles of the constant magnet are in the up and down position, and the N and S poles of the constant magnet are respectively aligned with the non-magnetic material.
  • Pre-treatment Wipe and sterilize the magnetic base storage tray, pipette, syringe holder and sterilization tray parts with disinfectant to ensure the magnetic base storage tray, pipette and syringe holder installed in the clean bench.
  • the sterilization tray will not change the aseptic environment in the clean bench; if it is used frequently, it will be placed in the ultra-clean bench for a long time, which can eliminate the sterilization of the disinfectant;
  • Installation of the magnetic base rotate the constant magnet rotary switch to the off position; at this time, the N and S poles of the constant magnet are in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials separated by the non-magnetic material, magnetic
  • the adsorption surface of the seat is not magnetic and can move freely; the magnetic base is placed at the desired position.
  • the specific operation is as follows: the magnetic seat adsorption surface is in contact with the vertical side plate of the iron in the ultra-clean platform, and the bottom surface of the magnetic seat and the bottom surface of the ultra-clean platform Contact; rotate the constant magnet to rotate the switch to the open position.
  • the N and S poles of the constant magnet are in the up and down position, and the N and S poles of the constant magnet are respectively aligned with the two soft magnetic materials separated by the non-magnetic material.
  • the magnetic field line is closed, and the magnetic seat adsorption surface is firmly adsorbed on the iron vertical side in the ultra clean table Board
  • Installation of horizontal components of the storage tray select the required height, install the height adjustment lock of the horizontal member of the storage tray; the specific steps are as follows: loosen the positioning lock fastening screw, and insert the cylindrical hole in the middle of the positioning lock into the column, select the To the required height, tighten the positioning lock fastening screw so that the silicone pad of the positioning lock is tightly engaged with the column; after the height positioning lock is installed, take the horizontal member of the storage tray, and insert the storage tray connecting sleeve into the column, and connect the sleeve.
  • the lower edge of the cylinder is located at the upper edge of the height positioning lock, and the installation and height positioning of the horizontal member of the storage tray are completed;
  • Pipette rack horizontal member installation select the required height, install the pipette rack horizontal member height positioning lock, the installation method is the same as the method of installing the storage tray horizontal member height positioning lock; after the height positioning lock is installed, The horizontal member of the pipette holder is taken, the pipette frame connecting sleeve is sleeved into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock, and the installation and height positioning of the horizontal member of the pipette holder are completed;
  • Installation of the horizontal component of the syringe holder select the required height, install the height adjustment lock of the horizontal height of the syringe holder, and the installation method is the same as the method of installing the height adjustment lock of the horizontal member of the storage tray; after the height positioning lock is installed, take the level of the syringe holder The component sleeve is sleeved into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the installation and height positioning of the horizontal component of the syringe holder;
  • Installation of the horizontal components of the sterilization tray select the required height, install the horizontal position lock of the horizontal position of the sterilization tray, and the installation method is the same as the method of installing the height adjustment lock of the horizontal member of the storage tray; after the height positioning lock is installed, the removal is performed.
  • the horizontal component of the bacteria tray, the sterilization tray connecting sleeve is sleeved into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the installation and height positioning of the horizontal components of the sterilization tray;
  • the order of installation of the above four horizontal members can be arbitrarily selected according to the needs of the experiment; the diameter of the inner ring of the connecting sleeve is slightly larger than the outer diameter of the column, so that the connecting sleeve can be freely rotated on the column without being allowed to A significant swing is generated on the column; thus, the installation of the magnetic base storage tray, the pipette, the syringe holder, and the sterilization tray in the ultra-clean platform is completed; for the sake of safety, the ultraviolet lamp is double-sterilized and the secondary sterilization is completed. After, turn off the UV lamp;
  • Placement of the storage item adjust the horizontal position of the storage tray horizontally according to the needs of the experiment; wherein the storage object placement board frame surrounds the circular storage object placement board and is 1 cm higher than the board surface of the storage object placement board, for preventing the storage object The falling off; according to the experiment needs to put or remove the small commonly used equipment including the pipette tip, the pipette tip, the alcohol cotton ball, the culture bottle cap, the lighter, the ophthalmology on the storage placing plate;
  • Pipette placement According to the experiment, rotate the horizontal position of the pipette holder; place the pipette vertically or remove the pipette from the bottom of the pipette. Since the root of the pipette extends to both sides, its width It is larger than the diameter of other parts of the pipette; the pipette part with a small width can be inserted into the lower groove of the pipette, and the root of the pipette with a large width is stuck above the pipe under the pipette, which realizes Vertical placement of the pipette on the pipette holder;
  • Placement of the syringe Rotate and adjust the horizontal position of the syringe holder according to the needs of the experiment; place the syringe horizontally to the syringe or remove the syringe;
  • Placement of plastic cell culture plate (abbreviated as culture plate): According to the needs of the experiment, horizontally rotate the plate to position the plate horizontally, and place the plate and the plate cover into the space surrounded by the non-slip frame, the culture hole and The inner surface of the culture plate cover should face the ultraviolet lamp; the ultraviolet lamp in the ultra-clean table should be turned on to sterilize them. After the sterilization process is finished, the ultraviolet lamp is turned off, and the culture plate cover is covered under aseptic conditions. Remove the sterilized culture plate from the bacteria tray and set aside;
  • Disassembly When removing the magnetic base storage tray, pipette, syringe holder, and sterilization tray from the clean bench, in the order from top to bottom, first vertically remove the column from the column and remove the horizontal component of the sterilization tray.
  • the method of taking out the syringe, the pipette and the horizontal member of the storage tray is the same as the method of removing the horizontal member of the sterilization tray, and the syringe is taken out,
  • the operation of the pipette and the storage tray height positioning lock is the same as the method of removing the sterilization tray height positioning lock; finally, the constant magnet rotary switch is turned to the off position, and the magnetic base can be taken out.
  • the invention can solve the above-mentioned experimental small equipment, pipettes and syringes in the ultra-clean platform by using a bracket on the premise that the ultra-clean bench space is not occupied (especially without occupying the mesa space in the center of the ultra-clean platform). Ordered placement problems within the station, and efficient sterilization of the UV lamp.
  • the vertical space position of the storage placing plate, the pipette shelf plate, the syringe rack plate and the horizontal placement plate of the culture plate can be respectively changed by adjusting the position of the horizontal member height positioning lock on the column.
  • the storage placement plate, the pipette holder plate, the syringe holder plate, and the culture plate can also be horizontally placed by adjusting the angles of the storage tray horizontal member, the pipette holder horizontal member, the syringe holder horizontal member, and the sterilization tray horizontal member.
  • the horizontal spatial position of the board It is conducive to the rapid, efficient and accurate operation of scientific research experiments in the ultra-clean platform. It can be seen that the magnetic base storage tray, pipette, syringe holder and sterilization tray for the ultra-clean platform of this application are the above-mentioned experimental small equipment which can be used in the ultra-clean platform, which has adjustable storage space, reasonable structure and convenient use. Pipettes, syringe placement, and culture plates are used to illuminate the sterilized device.
  • the horizontal member of the storage tray of the invention is a toughened flame-retardant reinforced polybutylene terephthalate composite, which has good toughness and excellent sterilization effect, and is particularly suitable for use in a clean bench.
  • Figure 1 is a front elevational view of the magnetic base.
  • FIG. 2 is a longitudinal sectional view of a magnetic seat.
  • Figure 3 is a top plan view of the horizontal member height positioning lock.
  • Figure 4 is a plan view of the horizontal member of the storage tray.
  • Figure 5 is a top plan view of the pipette holder horizontal member.
  • Figure 6 is a perspective view of the horizontal member of the syringe holder.
  • Figure 7 is a top plan view of the sterilizing tray horizontal member.
  • Chassis fixing screw Fix the column chassis to the upper surface of the magnetic base.
  • Magnetic seat adsorption surface The magnetic base is attracted to the vertical side plate of the iron in the clean bench.
  • Constant magnet rotary switch Turn the constant magnet to the off position, the magnetic force on the adsorption surface of the magnetic base disappears, and the magnetic base can be moved freely; the constant magnet is rotated to the open position, the magnetic base adsorption surface generates magnetic force, and the magnetic base is firm.
  • the adsorption of the ultra-clean bench is on the vertical side of the iron.
  • Magnetic seat Produces adsorption magnetic force.
  • Magnetic base It is in contact with the bottom surface of the clean bench to increase the stability of the magnetic base.
  • Constant magnet The source of magnetic generation.
  • Non-magnetic material Separate the soft magnetic material of the magnetic base into uniform upper and lower halves.
  • Soft magnetic material According to the position of the constant magnet, the magnetic seat respectively generates magnetization or demagnetization effect.
  • Positioning lock silicone pad It is combined with the magnetic base column to form a large static friction force, so that the positioning lock is well fixed at the height position required by the magnetic base column.
  • Positioning lock fastening screw Tighten the screw to make the positioning lock tightly fit on the column of the magnetic base; loosen the screw to remove the positioning lock to the magnetic base column, and move the positioning lock up and down.
  • Positioning lock body Forming the cylindrical space of the positioning lock and the magnetic base column.
  • Positioning lock hinge The occlusion cylindrical space of the positioning lock can be effectively opened and closed.
  • Storage tray horizontal arm connecting sleeve and storage tray.
  • Storage object placement board frame Prevent the falling of the storage items.
  • Storage object placement board The part where the storage item is placed.
  • Storage tray connection sleeve The storage tray horizontal member is fixed on the column of the magnetic base, and helps the storage tray horizontal member to achieve a large angle movement in the horizontal direction.
  • Pipette holder horizontal arm Connect the sleeve to the pipette holder.
  • Pipette holder Provides the forming portion of the lower channel of the pipette.
  • Pipette under the hanging slot The pipette can be placed vertically downwards.
  • Pipette holder connection sleeve Place the pipette holder horizontal member on the column of the magnetic base and help the pipette holder horizontal member to move at a large angle in the horizontal direction.
  • Syringe holder horizontal arm connecting the sleeve to the syringe holder plate.
  • Syringe holder plate Provides the forming portion of the horizontal placement slot of the syringe.
  • Syringe horizontal placement slot The syringe can be placed horizontally.
  • Syringe holder attachment sleeve The syringe horizontal member is placed over the post of the magnetic base and assists the syringe holder horizontal member in achieving a large angular movement in the horizontal direction.
  • Sterilization tray horizontal arm connecting sleeve and sterilization tray.
  • Non-slip off frame Prevents the plate from slipping off.
  • Sterilization tray connection sleeve The sterilizing tray horizontal member is placed on the column of the magnetic base and helps the sterilizing tray horizontal member to move at a large angle in the horizontal direction.
  • the utility model relates to a magnetic base storage tray, a pipettor, a syringe holder and a sterilization tray for a clean platform
  • the ultra-clean table comprises a magnetic seat for generating an adsorption magnetic force, and the side of the magnetic seat is for adsorbing the magnetic base in the ultra-clean
  • the magnetic seat adsorption surface of the iron vertical vertical plate on the platform, the bottom surface of the magnetic base is in contact with the bottom surface of the clean bench, the upper surface of the magnetic seat is fixed with the column bottom plate, the column is provided with the column on the column bottom;
  • the height of the four horizontal members is mounted on the column Positioning lock, above the four horizontal members, the height positioning locks respectively are arranged on the column, the horizontal member of the storage tray horizontally rotatable on the column, the horizontal member of the pipette holder, the horizontal member of the syringe holder, and the sterilization tray member;
  • the storage tray horizontal member includes a connecting sleeve for fitting the storage tray horizontal member on the column of the magnetic base, and assisting the storage tray horizontal member to achieve a large angular movement in the horizontal direction, and the connecting sleeve passes the horizontal arm
  • the storage tray is connected to the storage tray, and the storage tray is provided with a frame 1cm higher than the surface of the storage board for preventing the falling of the storage object.
  • the storage tray is used for accommodating the insertion or removal of the storage tray according to the experiment. Use small equipment;
  • the pipette holder horizontal member includes a pipette connection sleeve, and the pipette connection sleeve is used to sleeve the pipette holder horizontal member on the column of the magnetic base, and helps the horizontal level of the pipette holder to be horizontal
  • the pipette connection sleeve is connected to the pipette holder plate by the pipette horizontal arm, and the pipette holder plate is provided with an open pipette lower hanging groove, and the pipetting can be placed vertically downward.
  • the syringe holder horizontal member includes a syringe holder connecting sleeve for fitting the syringe holder horizontal member on the column of the magnetic base, and assisting the syringe holder horizontal member to achieve a large angular movement in the horizontal direction, the syringe holder
  • the connecting sleeve is connected to the syringe rack plate through the horizontal arm of the syringe holder, and the syringe rack plate is provided with a horizontal slot for the syringe to horizontally place the syringe;
  • the sterilizing tray horizontal member comprises a sterilizing tray connecting sleeve, and the sterilizing tray connecting sleeve is used for tying the sterilizing tray horizontal member on the column of the magnetic base, and helping the sterilizing tray horizontal member to achieve a horizontal direction The angle is moved, and the sterilizing tray connecting sleeve is connected with the horizontal placing plate of the culture plate of the cell culture plate on which the plastic material is placed through the horizontal arm of the sterilizing tray, and the anti-skid frame is arranged around the horizontally placed plate of the culture plate to prevent the plate from slipping off;
  • the storage tray horizontal member is a toughened flame-retardant reinforced polybutylene terephthalate composite composed of the following weight percentage components:
  • Toughener is 1.5 to 10% of LLDPE
  • the bromide is one or both of decabromodiphenylethane and a brominated epoxy having a molecular weight of 10,000 to 50,000.
  • the horizontal member height positioning lock comprises a positioning lock body for forming a positioning cylindrical space of the positioning lock and the magnetic base column; the positioning lock body is composed of two left and right pieces, and the left and right two pieces are connected by a positioning lock hinge.
  • the cohesive cylindrical space of the positioning lock can be effectively opened and closed; the left and right open ends of the positioning lock body are provided with positioning locking screws, and the positioning locking screw is tightened to make the positioning lock tightly fit on the column of the magnetic base. Loosen the positioning lock fastening screw, remove the positioning lock to the magnetic base column, and move the positioning lock up and down.
  • a positioning lock silicone pad is arranged in the positioning lock body, and the positioning lock silicone pad and the magnetic base column are engaged, so that the positioning lock is fixed at a height position required by the magnetic base column.
  • the magnetic base comprises a soft magnetic material, wherein the soft magnetic material is provided with a non-magnetic material that separates the soft magnetic material into uniform upper and lower halves, and a constant magnet and a constant magnet are arranged in the cylindrical space surrounded by the soft magnetic material.
  • the N and S poles of the constant magnet are in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials separated by the non-magnetic material, magnetic
  • the adsorption surface of the seat is not magnetic and can move freely; when the constant magnet rotates the switch to the open position, the N and S poles of the constant magnet are in the up and down position, and the N and S poles of the constant magnet are respectively aligned with the non-magnetic material.
  • Pre-treatment Wipe and sterilize the magnetic base storage tray, pipette, syringe holder and sterilization tray parts with disinfectant to ensure the magnetic base storage tray, pipette and syringe holder installed in the clean bench.
  • the sterilization tray will not change the aseptic environment in the clean bench; if it is used frequently, it will be placed in the ultra-clean bench for a long time, which can eliminate the sterilization of the disinfectant;
  • a closed circuit is formed in the soft magnetic material (10), and the magnetic seat adsorption surface (4) is free from magnetism and can move freely; the magnetic base (1-7) is placed at a desired position, and the specific operation is as follows: the magnetic seat adsorption surface (4) In contact with the vertical side plate of the iron in the clean bench, the bottom surface of the magnetic seat (7) is in contact with the bottom surface in the clean table; the rotating constant magnet rotates the switch to the open position, and the N and S poles of the constant magnet are in the up and down position.
  • the N and S poles of the constant magnet are respectively aligned with two soft magnetic materials separated by a non-magnetic material, from the N pole to the soft magnetic material to the iron vertical side plate in the ultra clean table to another soft magnetic material to S pole, forming a magnetic line close, the magnetic seat adsorption surface is firmly adsorbed on the iron vertical side plate in the ultra clean bench;
  • Installation of horizontal components of the storage tray Select the required height, install the horizontal height lock (11-14) of the storage tray horizontal member; the specific steps are as follows: loosen the positioning lock fastening screw (12), the column in the middle of the positioning lock Insert the hole into the column (1), select the required height, tighten the positioning lock fastening screw, and make the silicone pad (11) of the positioning lock tightly fit with the column; after the height positioning lock is installed, take the horizontal member of the storage tray (15-18), the storage tray connecting sleeve (18) is sleeved into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the installation and height positioning of the horizontal member of the storage tray;
  • Pipette rack horizontal member installation select the required height, install the pipette rack horizontal member height positioning lock, the installation method is the same as the method of installing the storage tray horizontal member height positioning lock; after the height positioning lock is installed, Take the pipette frame horizontal member (19-22), insert the pipette holder connecting sleeve (22) into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the horizontal member of the pipette holder. Installation and height positioning;
  • Installation of the horizontal component of the syringe holder select the required height, install the height adjustment lock of the horizontal height of the syringe holder, and the installation method is the same as the method of installing the height adjustment lock of the horizontal member of the storage tray; after the height positioning lock is installed, take the level of the syringe holder
  • the member (23-26), the syringe holder connecting sleeve (26) is sleeved into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the installation and height positioning of the horizontal member of the syringe holder;
  • Installation of the horizontal components of the sterilization tray select the required height, install the horizontal position lock of the horizontal position of the sterilization tray, and the installation method is the same as the method of installing the height adjustment lock of the horizontal member of the storage tray; after the height positioning lock is installed, the removal is performed.
  • the tray horizontal member (27-30), the sterilization tray connecting sleeve (30) is sleeved into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the installation and height positioning of the horizontal member of the sterilization tray;
  • the order of installation of the above four horizontal members can be arbitrarily selected according to the needs of the experiment; the diameter of the inner ring of the connecting sleeve is slightly larger than the outer diameter of the column, so that the connecting sleeve can be freely rotated on the column without being allowed to A significant swing is generated on the column; thus, the installation of the magnetic base storage tray, the pipette, the syringe holder, and the sterilization tray in the ultra-clean platform is completed; for the sake of safety, the ultraviolet lamp is double-sterilized and the secondary sterilization is completed. After, turn off the UV lamp;
  • Placement of the storage item adjust the horizontal position of the storage tray horizontally according to the needs of the experiment; wherein the storage object placement board frame (16) surrounds the circular storage object placement board (17) and is higher than the board surface of the storage object placement board by 1 cm. It is used to prevent the falling off of the storage item; according to the experiment, the small commonly used small equipment including the dropper tip, the straw tip, the alcohol cotton ball, the culture bottle cap, the lighter, the ophthalmology is placed on the storage placing plate;
  • Pipette placement According to the experimental needs, rotate the horizontal position of the pipette holder plate (20); place the pipette vertically or remove the pipette from the pipette under the trough (21), since the root of the pipette is facing two Side stretched, so its width is larger than the diameter of other parts of the pipette; the pipette part with a small width can be inserted into the lower groove of the pipette, while the root of the pipette with a large width is stuck under the pipette Above the trough, the vertical placement of the pipette on the pipette holder is achieved;
  • Syringe placement Rotate the horizontal position of the syringe holder plate (24) according to the needs of the experiment; place the syringe horizontally (25) horizontally or remove the syringe;
  • culture plate Placement of plastic cell culture plate (hereinafter referred to as culture plate): According to the experiment, horizontally rotate the plate to position the plate horizontally (28), and place the plate and the plate cover in a non-slip-off frame (29). In the space, the inner surface of the culture hole and the culture plate cover should face the ultraviolet lamp; the ultraviolet lamp in the ultra-clean stage is turned on to sterilize them, and after the sterilization process is finished, the ultraviolet lamp is turned off, and under aseptic conditions, the cover is covered. Raise the plate cover, remove the sterilized culture plate from the sterilization tray, and set aside;
  • Disassembly When removing the magnetic base storage tray, pipette, syringe holder, and sterilization tray from the clean bench, in the order from top to bottom, first vertically remove the column from the column and remove the horizontal component of the sterilization tray.
  • the method of taking out the syringe, the pipette and the horizontal member of the storage tray is the same as the method of removing the horizontal member of the sterilization tray, and the syringe is taken out,
  • the operation of the pipette and the storage tray height positioning lock is the same as the method of removing the sterilization tray height positioning lock; finally, the constant magnet rotary switch is turned to the off position, and the magnetic base can be taken out.

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  • Devices For Use In Laboratory Experiments (AREA)

Abstract

Un plateau de stockage de base magnétique, un porte-pipette, un porte-seringue et un plateau de stérilisation pour banc stérile, le banc stérile comprenant une base magnétique (6) qui produit une force d'attraction magnétique, une surface latérale de la base magnétique (6) étant une surface d'attraction de base magnétique (4) qui fixe la base magnétique à une plaque latérale verticale ferreuse à l'intérieur du banc stérile, une surface inférieure de base magnétique (7) étant en contact avec une surface inférieure à l'intérieur du banc stérile, une plaque inférieure de colonne (3) étant montée à demeure sur une surface supérieure de la base magnétique (6), une colonne (1) étant disposée sur la plaque inférieure de colonne (3). Quatre verrous de positionnement de hauteur de pièces horizontales sont montés sur la colonne (1), et une pièce horizontale de plateau, une pièce horizontale de porte-pipette, une pièce horizontale de porte-seringue et une pièce horizontale de plateau de stérilisation, qui sont manchonnées sur la colonne (1) et qui peuvent tourner horizontalement sur la colonne (1), sont respectivement agencées au-dessus des quatre verrous de positionnement de hauteur de pièces horizontales. La présente invention est raisonnable en termes de structure, facile à utiliser et ajustable en termes d'espace de mise en place.
PCT/CN2018/082743 2018-04-12 2018-04-12 Plateau de stockage à base magnétique, porte-pipette, porte-seringue et plateau de stérilisation pour banc stérile WO2019119683A1 (fr)

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PCT/CN2018/082743 WO2019119683A1 (fr) 2018-04-12 2018-04-12 Plateau de stockage à base magnétique, porte-pipette, porte-seringue et plateau de stérilisation pour banc stérile
ZA2019/06016A ZA201906016B (en) 2018-04-12 2019-09-11 Magnetic base storage tray, pipettor holder, syringe holder and sterilisation tray for clean bench

Applications Claiming Priority (1)

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PCT/CN2018/082743 WO2019119683A1 (fr) 2018-04-12 2018-04-12 Plateau de stockage à base magnétique, porte-pipette, porte-seringue et plateau de stérilisation pour banc stérile

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WO2019119683A1 true WO2019119683A1 (fr) 2019-06-27

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