WO2019117701A3 - Método para la detección y cuantificación simultánea de virus de epstein-barr, citomegalovirus, herpesvirus humano 6, herpesvirus humano 7 y virus de sarcoma de kaposi mediante reacción en cadena de la polimerasa en tiempo real, multiplex - Google Patents

Método para la detección y cuantificación simultánea de virus de epstein-barr, citomegalovirus, herpesvirus humano 6, herpesvirus humano 7 y virus de sarcoma de kaposi mediante reacción en cadena de la polimerasa en tiempo real, multiplex Download PDF

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Publication number
WO2019117701A3
WO2019117701A3 PCT/MX2019/000011 MX2019000011W WO2019117701A3 WO 2019117701 A3 WO2019117701 A3 WO 2019117701A3 MX 2019000011 W MX2019000011 W MX 2019000011W WO 2019117701 A3 WO2019117701 A3 WO 2019117701A3
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Prior art keywords
human herpesvirus
kaposi
epstein
cytomegalovirus
multiplex
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PCT/MX2019/000011
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English (en)
French (fr)
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WO2019117701A2 (es
Inventor
Ezequiel M. FUENTES PANANÁ
Abigail MORALES SÁNCHEZ
Yessica SÁNCHEZ PONCE
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Hospital Infantil De México "Federico Gómez"
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Priority to US16/772,714 priority Critical patent/US20210032710A1/en
Publication of WO2019117701A2 publication Critical patent/WO2019117701A2/es
Publication of WO2019117701A3 publication Critical patent/WO2019117701A3/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se relaciona con un método para la detección y cuantificación múltiple y simultánea de cualquier combinación de los herpesvirus humanos de los géneros beta y gamma: Virus de Epstein-Barr (VEB), Citomegalovirus (CMV), Herpesvirus humano tipo 6 (HVH6), Herpesvirus humano tipo 7 (HVH7) y Virus de Sarcoma de Kaposi (VSK) y del gen endógeno humano beta-actina mediante reacción de amplificación de ADN usando la reacción en cadena de la polimerasa múltiplex en tiempo real.
PCT/MX2019/000011 2017-12-14 2019-02-14 Método para la detección y cuantificación simultánea de virus de epstein-barr, citomegalovirus, herpesvirus humano 6, herpesvirus humano 7 y virus de sarcoma de kaposi mediante reacción en cadena de la polimerasa en tiempo real, multiplex WO2019117701A2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/772,714 US20210032710A1 (en) 2017-12-14 2019-02-14 Method For The Simultaneous Detection And Quantification Of Epstein-Barr Virus, Cytomegalovirus, Human Herpesvirus 6, Human Herpesvirus 7 and Kaposi's Sarcoma Virus Using A Multiplex, Real-Time Polymerase Chain Reaction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX2017016321A MX2017016321A (es) 2017-12-14 2017-12-14 Metodo para la deteccion y cuantificacion simultanea de virus de epstein-barr, citomegalovirus, herpesvirus humano 6, herpesvirus humano 7 y virus de sarcoma de kaposi mediante reaccion en cadena de la polimerasa en tiempo real, multiplex.
MXMX/A/2017/016321 2017-12-14

Publications (2)

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WO2019117701A2 WO2019117701A2 (es) 2019-06-20
WO2019117701A3 true WO2019117701A3 (es) 2019-08-29

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PCT/MX2019/000011 WO2019117701A2 (es) 2017-12-14 2019-02-14 Método para la detección y cuantificación simultánea de virus de epstein-barr, citomegalovirus, herpesvirus humano 6, herpesvirus humano 7 y virus de sarcoma de kaposi mediante reacción en cadena de la polimerasa en tiempo real, multiplex

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US (1) US20210032710A1 (es)
MX (1) MX2017016321A (es)
WO (1) WO2019117701A2 (es)

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CN113930436A (zh) * 2021-10-28 2022-01-14 吉林正业生物制品股份有限公司 双标准曲线狂犬病毒阳性标准品质粒及其在定量检测狂犬病毒中的用途
CN115029478B (zh) * 2021-11-16 2023-12-22 江汉大学 一种人巨细胞病毒的mnp标记位点、引物组合物、试剂盒及其应用

Citations (2)

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WO2004016219A2 (en) * 2002-08-14 2004-02-26 The Board Of Regents Of The University Of Texas System Clinical assays for the detection and typing of human herpesviruses
WO2007113388A2 (en) * 2006-03-31 2007-10-11 Licentia Oy Method and microarray for detecting herpesviruses

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US20040038282A1 (en) * 2000-10-27 2004-02-26 Applera Corporation Isolated human secreted proteins, nucleic acid molecules encoding human secreted proteins, and uses thereof
US9402895B2 (en) * 2007-08-15 2016-08-02 University of Pittsburg—Of the Commonwealth System of Higher Education Method of enhancing KSHV LANA1 immunogenicity
US8951723B2 (en) * 2009-02-12 2015-02-10 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Serological screening for HHV-8 infection using antigen mixtures
US20120122700A1 (en) * 2010-11-11 2012-05-17 University Of South Florida Materials and methods for determining subtelomere dna sequence

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2004016219A2 (en) * 2002-08-14 2004-02-26 The Board Of Regents Of The University Of Texas System Clinical assays for the detection and typing of human herpesviruses
WO2007113388A2 (en) * 2006-03-31 2007-10-11 Licentia Oy Method and microarray for detecting herpesviruses

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FUJIMURO MASAHIRO ET AL.: "Multiplex PCR-based DNA array for simultaneous detection of three human herpesviruses, EVB, CMV and KSHV", EXPERIMENTAL AND MOLECULAR PATHOLOGY, vol. 80, no. 2, 31 March 2006 (2006-03-31), pages 124 - 131, XP024944818, ISSN: 0014-4800 *
KIMURA HIROSHI ET AL.: "Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 37, no. 1, 31 December 1998 (1998-12-31), pages 132 - 136, XP002548794, ISSN: 0095-1137 *
ONO Y ET AL.: "SIMULTANEOUS MONITORING BY REAL-TIME POLYMERASE CHAIN REACTION OF EPSTEIN-BARR VIRUS, HUMAN CYTOMEGALOVIRUS, AND HUMAN HERPESVIRUS-6 IN JUVENILE AND ADULT LIVER TRANSPLANT RECIPIENTS et al.", TRANSPLANTATION PROCEEDINGS, vol. 40, no. 10, 1 December 2008 (2008-12-01), pages 3578 - 3582, XP025800005, ISSN: 0041-1345 *
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WO2019117701A2 (es) 2019-06-20
MX2017016321A (es) 2019-01-14
US20210032710A1 (en) 2021-02-04

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