WO2019088774A2 - Dynastid beetle larva extract and method for preparing same - Google Patents

Abstract

The present application relates to a dynastid beetle larva extract and a method for preparing same.

Description

Extract of Longevity beetle larvae and method for manufacturing the same

The present application relates to a long beetle larvae extract and a method for producing the same.

Since the United Nations report, which recommends insects as food in 2013, there has been a growing interest in food and the environment in the future, and research on edible insects is actively being conducted. Insects contain a large amount of unsaturated fatty acids and amino acids, as well as various vitamins, minerals, chitosan and contains nutrients.

Among them, Allomyrina dichotoma ) is an insect belonging to the beetle chinensis. The larva of this longevity beetle is a locust in Korea with a grasshopper, edible pupa, a white mackerel, a brown duck larva, a white spotted mackerel and a pair of crickets. It is an insect registered as a raw material. The long-bred beetle larvae have high quality nutrients such as carbohydrate 9.4%, protein 11.3% and fat 6.1%. However, in addition to being unfamiliar with ingesting long-lived beetle larvae as raw materials, Due to the fat content in beetle larvae, the shape of the powder is not spoiled, and there are already (insect peculiar peculiar taste) and bad taste (insect peculiar peculiar smell), making it difficult for ordinary consumers to easily ingest.

Thus, attempts have been made to utilize useful nutrients while eliminating the dislike of insect food ingredients such as longevity beetle larvae. In particular, researches have been conducted to improve nutritional components and flavors such as unsaturated fatty acids in insects. In particular, there has been an attempt to use a functional material such as the Korean Patent Registration No. 1,533,600 as a long-bred beetle larva, but studies for use as a food by improving the flavor have not been satisfactorily conducted. .

[Prior Art Literature]

(Patent Document 1) Korean Patent Publication No. 1,533,600 (Jul. 20, 2015)

One object of the present invention is to provide a long-beetle larva extract having improved flavor so that nutritional ingredients contained in larvae of beetle larvae can be used for food, and a method for producing the same.

Another object of the present application is to provide a composition and a food containing the long-bred beetle larvae extract.

To this end, one aspect of the present application provides a method for preparing a long beetle larva extract comprising the step of treating a long-lived beetle larva with a proteolytic enzyme.

Another aspect of the present application provides a long-beetle larva extract having a hexanal content of more than 0 mg / L and not more than 0.7 mg / L, which is produced by the method for manufacturing the long-beetle larvae extract.

In addition, another aspect of the present application provides a composition for food materials comprising the long-lived beetle larvae extract.

The longevity beetle larvae extract prepared according to the method of the present invention for preparing Longevity beetle larvae extract and the compositions and foods containing the same have not only improved flavor but also have a high free amino acid content and are excellent in antioxidant and anti- Show efficacy.

However, the effects of the present application are not limited to the above-mentioned effects, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.

Fig. 1 is a flow chart showing a method for producing a long beetle larva extract of the present application.

Fig. 2 is a table showing an appropriate treatment condition of proteolytic enzyme treated in the process of producing the long beetle larva extract of the present application.

Fig. 3 is a graph showing the results of confirming the electron donating ability of the long-beetle larvae larvae extract of the present application as confirmed in Experimental Example 2 by DPPH radical scavenging activity test.

4 is a table showing the EC ₅₀ (ppm) value of the long-beetle larvae extract of the present application as confirmed in Experimental Example 2. FIG.

FIG. 5 is a graph showing the inhibitory effect of Nitrite production on the RAW 264.7 macrophages of the present long-beetle larvae extract of the present application as confirmed in Experimental Example 3. FIG. 5a is a graph showing the inhibitory effect of Nirite formation on the production method (with or without hot water extraction, And FIG. 5B is a graph showing the effect of inhibiting Nirite formation by enzyme treatment and concentration difference.

FIG. 6 is a graph showing the cell survival analysis results of RAW 264.7 macrophages of Long-beetle beetle larvae extract of the present application as confirmed in Experimental Example 4. FIG.

FIG. 7 is a graph showing changes in the content of hexanal components of the long-beetle larvae extract according to the present invention as determined in Experimental Example 5. FIG.

Hereinafter, the present application will be described in detail.

One aspect of the present application provides a long-bred beetle larvae extract and a method of making the same.

The longevity beetle larvae extract of the present application is prepared by treating proteolytic enzyme in a larval beetle larva.

First, the proteolytic enzyme is applied to the larval beetle larvae.

The step of treating proteolytic enzyme in the long-beetle beet larva is a method of decomposing a protein, which is one of the useful components contained in the long-beetle beet larva, into a free amino acid or polypeptide to make it easy to be absorbed into the body, It is a process to remove imaged odor and to reduce the sense of foreign body.

Although the protease is not particularly limited, it may be one that is widely used commercially in consideration of economical efficiency. Specific examples thereof include pepsin, trypsin, Flavourzyme, Protamex, papain, alpha Chymotrypsin, pancreatase and the like can be used. More specifically, it can be flavorzyme, protease, and the like. The Plastic Bor atom is Aspergillus duck material (Aspergillus oryzae , and has both endoprotease characteristics and exoprotease characteristics. The protease is a protease derived from Bacillus subtilis ( Bacillus subtilis), and endoprotease endoprotease (see Fig. 2).

The protease is used at a concentration of 0.0001 to 5 parts by weight, specifically 0.01 to 3 parts by weight, more specifically 0.1 to 2 parts by weight, relative to 100 parts by weight of the long-beetle larvae It can be treated with a negative concentration. And wherein the proteolytic enzyme can be treated for 5 minutes to 5 hours, specifically 30 minutes to 2 hours, more specifically 1 hour to 1.5 hours. If the treatment concentration of the protease is less than 0.0001 parts by weight based on 100 parts by weight of the larvae of the long-beetle beetle larvae or the treatment time of the protease is less than 5 minutes, the protein is not sufficiently decomposed, However, there is a problem that it is impossible to sufficiently reduce the already existing, off-odor, or foreign matter of the larval beetle larva, and when the treatment concentration of the protease exceeds 5 parts by weight with respect to the total 100 parts by weight of the larval beetle larvae, If the time exceeds 5 hours, there is a problem that the cost is excessively increased or the process economical efficiency is lowered.

On the other hand, before treating the proteolytic enzyme as described above, the longevity beetle larva may be further subjected to a step of fasting and then pulverizing.

The step of raising the long beetle larva is a process for removing imagenous odor or foreign body inherent to the long beetle larva by lowering the content of feed and excrement remaining in the larva of the long beetle larva. The longevity beetle larvae are grown by maintaining proper temperature and humidity. They are mainly grown by sawdust or pushing. When the longevity beetle larvae are processed without fasting, the larvae of beetle larvae are processed However, strong already, odor or foreign body remains, so that imine odor or foreign body inherent to the long beetle larva can be further reduced through the above-described fasting process. The abstinence step may be performed for 1 to 120 hours, specifically 1 to 96 hours, 1 to 72 hours, or more specifically 12 to 120 hours, 12 hours to 96 hours, 12 hours to 72 hours, or specifically 24 hours to 120 hours, 24 hours to 96 hours, 24 hours to 72 hours. If the above-mentioned fasting time is shorter than 1 hour, the feed and excrement of the larvae of the long-lived beetle larva can not be sufficiently removed, and foreign matter or a foreign object may be left. If the above-mentioned fasting time exceeds 120 hours, There is a problem that can be. In addition, before the abstinence step, the long-lived beetle larvae are grown in a grain diet such as wheat flour instead of sawdust or pushing, thereby further reducing the already-existing, odorous or foreign matter of the larval beetle larvae.

In addition, the long-lived beetle larvae that have been fasted as described above may be pulverized to improve the efficiency of the protease before the protease is treated. At this time, the pulverization may be performed by cutting or decomposing the fasted larvae of the long-lived beetle larvae, and then selectively pulverizing only necessary parts, or crushing all parts without separate cutting or decomposition processes. In addition, a certain amount of water may be added to facilitate the pulverization and to further improve the efficiency of the proteolytic enzyme. 100 parts by weight to 3000 parts by weight, preferably, 100 parts by weight to 100 parts by weight of the fasted larvae of the long- Specifically, 200 to 2500 parts by weight, and more specifically, 400 parts by weight to 2000 parts by weight of water may be added. When the amount of water to be added is less than 100 parts by weight, the milling efficiency of the larval beetle larvae may not be improved. When the amount of water added is more than 3000 parts by weight, the solid content is low, There is a problem that the process economy is poor. Further, before treating the protease, the nutritional components can be further extracted from the pulverized product as described above by a conventional extraction method. For example, a hydrothermal export method can be applied, and in this case, it can be extracted at a temperature of 70 ° C to 150 ° C, specifically 80 ° C to 120 ° C, for 5 minutes to 60 minutes, specifically, 10 minutes to 30 minutes.

In addition, in order to further improve the efficiency of the proteolytic enzymes, the pulverized product of the long-lived beetle larva as described above may be further subjected to centrifugation and obtaining a supernatant before the proteolytic enzyme treatment. As a result, the fat and sediment can be excluded from the pulverized product of the larval beetle larvae, and the water-soluble component and the protein component can be separated. When the centrifugation and the supernatant are obtained as described above, the proteolytic enzyme is treated in the supernatant obtained through the above process. The centrifugation and supernatant obtaining step may be performed by centrifuging the pulverized long beetle larvae at 3000 rpm to 8000 rpm for 10 minutes to 30 minutes, and when the pulverized product of the long beetle larva is not sufficiently separated in the condition out of the range, There is a problem that the economy is poor.

On the other hand, after the proteolytic enzyme is treated on the long-lived beetle larva as described above, the enzyme-treated product may be further subjected to one or more steps of heating and cooling, filtering, concentrating and lyophilizing .

The step of heating and cooling the enzyme-treated product is a process for sterilizing the microorganisms while improving the stability as a food while inactivating proteolytic enzymes in the enzyme-treated product. The heating step may be carried out at a temperature of 90 to 150 ° C, specifically 95 to 130 ° C, more specifically 100 to 120 ° C, for 10 minutes to 120 minutes, specifically 20 to 60 minutes, For 30 minutes to 40 minutes. If the heating step is carried out at a temperature of less than 90 ° C or is carried out at a time of less than 10 minutes, inactivation of the proteolytic enzyme or sterilization of the microorganism may not be sufficiently performed, , The nutritional components may be destroyed. Further, after the heating step as described above, the cooling step may be performed. The cooling step is carried out at a temperature of 0 ° C to 10 ° C, specifically 1 ° C to 7 ° C, more specifically 3 ° C to 5 ° C, for 30 minutes to 120 minutes, specifically 45 minutes to 90 minutes, May be carried out for a time of from 50 minutes to 70 minutes. If the cooling step is carried out at a temperature of more than 10 DEG C or is carried out at a time of less than 30 minutes, the enzyme treatment may not be sufficiently cooled, and it may be carried out at a temperature of less than 0 DEG C or at a time of more than 120 minutes There is a possibility that the process economical efficiency may deteriorate.

In addition, the step of filtering the enzyme-treated product is a process for further improving the efficiency of pulverization as described above while reducing the foreign body sensation derived from the larval beetle larvae. In the enzyme-treated product, only the aqueous soluble supernatant A conventional filtration method such as a filter or the like can be used. For example, the enzyme-treated product can be filtered with a filter having a size of 0.2 mu m to 5 mu m. Outside of the above range, the enzyme-treated product may not be sufficiently separated or the process economy may be deteriorated.

In addition, the step of concentrating the enzyme-treated product is a step for further increasing the availability of the food material by increasing the content of the solid component and can be carried out through a concentration method that is generally used in the food field. For example, it can be concentrated under the conditions of 40 to 60 DEG C, 10 to 100 rpm and 20 to 100 mbar using a conventional concentrator. The concentration step may be performed such that the concentration of the enzyme-treated product is from 5 brix to 40 brix, specifically from 10 brix to 30 brix. If the concentration is outside the above range, the freeze-drying step may not be easy or the process economical efficiency may deteriorate. On the other hand, when both the heating and cooling steps and the concentration step are carried out, the enzyme-treated product is subjected to a simple deactivation of proteolytic enzymes at a temperature of 90 ° C or more for 10 minutes or more, The step may be carried out after the concentration step.

The lyophilization step may be performed by lyophilizing and pulverizing the enzymatically treated material, which is easy to store as a food material and applicable to various products. The powder obtained by lyophilization is lower in fat content than that obtained by drying in a general hot air drying method and then pulverized, so that the powder is not clumped and a more fine powder can be obtained.

By treating the long-lived beetle larva with the proteolytic enzyme as described above, it is possible to produce an extract of the long-lived beetle larva having a low fat content and a low odor and a small foreign matter.

Particularly, the long-beetle larvae extract of the present invention produced as described above contains 0 mg / L to 0.7 mg / L or less, specifically 0 mg / L to 0.5 mg L or less, 0 mg / L to 0.3 mg / L or less, 0 mg / L to 0.1 mg / L or less, 0 mg / L to 0.08 mg / L or less, or 0 mg / L to 0.07 mg / L or less .

In addition, the long-beetle larvae extract of the present invention produced as described above contains 0.1 mg / mL to 1.0 mg / mL of L-tyrosine, which is one of the amino acids produced by the hydrolysis of proteins by the protein hydrolyzing enzyme As shown in FIG. In addition to L-tyrosine as described above, the longevity beet larva extract of the present application contains a high content of other free amino acids or polypeptides in a form readily absorbable in the body.

In addition, the longevity beetle larva extract of the present invention produced as described above has oxidation and anti-inflammatory effects, but does not significantly affect cell death in anti-inflammatory action (see Examples 2 to 4).

The longevity beetle larva extract of the present application as described above can be prepared in powder, granule or liquid form and can be used or included as a component of food composition or food.

Hereinafter, the present invention will be described in detail with reference to Production Examples and Experimental Examples.

However, the following Production Examples and Experimental Examples are intended to illustrate the present invention specifically, and the contents of the present invention are not limited by the following Production Examples and Experimental Examples.

Manufacturing example  1: Preparation of Extract of Long-beetle beetle larvae

(1) Step of breeding long-beetle larvae

The larvae of 5-6 months old rabbits grown in sawdust were changed to flour before use and were expelled for 48 hours to expel the excreta, then washed and stored frozen.

(2) a step of crushing a long beetle larva

300 parts by weight of water was added to 100 parts by weight of the fasted larvae of the long-bred beetle larvae, and the mixture was pulverized to prepare a liquid form which was easy to be extracted and treated with enzymes.

(3) centrifuging the pulverized product

The pulverized product is centrifuged at 6000 rpm for 20 minutes, and only the supernatant is taken.

(4) Step of treating proteolytic enzyme in supernatant

Protease protease Flavourzyme or Protamex was added to the supernatant obtained by centrifugation at a concentration of 0.1%, 0.25%, 0.5%, 0.75%, and 1%, and flavorzyme was added at a concentration of 50 (Protamex) at a temperature of 60 ° C for 1, 2, 3, 4 or 5 hours, or 1% of flavorzyme and 1% of proteasma are added together at a temperature of 55 ° C For 1, 2, 3, 4, and 5 hours, and then the protease was inactivated at a temperature of 100 DEG C for 10 minutes.

(5) Step of sterilizing and cooling enzyme water of long beetle larva

The enzyme-treated solution was sterilized at 100 ° C for 30 minutes, cooled at 4 ° C for 60 minutes, and allowed to be stored for a long time under refrigeration conditions.

Manufacturing example  2: Preparation of Longevity beetle larvae extract

(1) controlling the diet of long-beetle larvae

The larvae of 5-6 months old rabbits grown in a pressurized diet were changed to a flour diet before use, and they were fasted for 48 hours to expel excreta, then washed and stored frozen.

(2) a step of crushing a long beetle larva

300 parts by weight of water was added to 100 parts by weight of the fasted larvae of the long-bred beetle larvae, and the mixture was pulverized to prepare a liquid form which was easy to be extracted and treated with enzymes.

(3) Extracting the pulverized product

The pulverized material is extracted at 100 DEG C for 20 minutes to extract water-soluble nutrients.

(4) Step of centrifuging the extract

The extract is centrifuged at 6000 rpm for 20 minutes, and only the supernatant is taken.

(5) Step of treating proteinase in supernatant

Protease protease Flavourzyme or Protamex was added to the supernatant obtained by centrifugation at a concentration of 0.1%, 0.25%, 0.5%, 0.75%, and 1%, and flavorzyme was added at a concentration of 50 (Protamex) at a temperature of 60 ° C for 1, 2, 3, 4 or 5 hours, or 1% of flavorzyme and 1% of proteasma are added together at a temperature of 55 ° C For 1, 2, 3, 4, and 5 hours, and then the protease was inactivated at a temperature of 100 DEG C for 10 minutes.

(6) Stage of sterilization and cooling of enzyme treatment liquid of beetle larva

The enzyme-treated solution was sterilized at 100 ° C for 30 minutes, cooled at 4 ° C for 60 minutes, and allowed to be stored for a long time under refrigeration conditions.

Experimental Example  1: Determination of L-tyrosine content by enzyme type and treatment conditions

The content of hydrolyzate according to hydrolysis time was determined by measuring L - tyrosine to determine the optimum enzyme concentration and reaction time for enzymatic hydrolysis of the larvae. Experiments were carried out with 0.1, 0.25, 0.5, 0.75 and 1% of flavorzyme and protease enzymes, respectively, and the reaction times were set to 1, 2, 3, 4 and 5 hours. The presence or absence of the water-soluble nutrients and the hydrolyzate content of the hydrolyzate were also determined by measuring L-tyrosine. In addition to the difference in the presence or absence of the water-soluble nutrient extraction step, the experiment was conducted by mixing the flavorzyme and the proteasma with 1% each and setting the reaction time to 1, 2, 3, 4, and 5 hours.

As a result, it was found that hydrolyzing ability of flavorzyme and protease in the case of single enzyme treatment and in the case of mixed treatment of flavorzyme and protease in the mixed treatment of flavorzyme and protease The hydrolysis efficiency of flavorzyme and protease enzyme was similar to that of protease, and hydrolysis was improved with increasing concentration and time. In terms of reaction time, the hydrolysis was observed to proceed rapidly from 1 hour after the enzyme treatment, and there was a slight increase thereafter, but the difference was not significant. Therefore, 1-5 hours of reaction time is required when a single enzyme of flavorzyme and protease is treated, and when 1-3 times of reaction time is applied when flavorzyme and protease are mixed, .

In the case of mixed treatment of flavor and protease, water - soluble nutrients were extracted from the groundwater beet larvae and centrifuged for enzyme hydrolysis.

In Experimental Examples 2 to 5 below, experiments were carried out using an extract obtained by mixing 1% of flavorzyme and 1% of proteasma and treating at 55 ° C for 2 hours.

Experimental Example  2: Long-beetle larvae extract Electron donating ability  Measure

The electron donating ability was measured using the reducing power of DPPH (1,1 diphenyl-2-picrylhydrazyl). 100 μl of a 0.2 mM DPPH solution was added to 100 μl of a 98 to 50000 ppm long water beetle larvae extract according to the production method according to whether or not there was hot water extraction and whether or not the enzyme treatment was carried out. The reaction was allowed to proceed at room temperature for 30 minutes and the absorbance was measured at 525 nm 3 and 4, respectively.

As a result, as shown in Fig. 3, it was confirmed that the extract prepared by another production method had an antioxidant ability, except that the water-soluble nutritional component of the larval water beetle larvae (longevity) was extracted, As a result, it was confirmed that when the proteolytic enzyme was treated without extracting the water-soluble nutrients of the larval beetle larvae, the EC ₅₀ value was 3953 ppm, indicating excellent antioxidant ability.

Experimental Example  3: Inhibition of NO production by the larval larvae extract

In order to analyze the anti-inflammatory effect, RAW 264.7 cells induced by inflammatory response through LPS (Lipopolysaccharide) were treated with a long-bred beetle larvae extract to determine the amount of NO produced by measuring the amount of NO ₂ ^- present in the cell culture medium .

Raw 264.7 cells were cultured in a medium containing 100 units / ml of penicillin-streptomycin and 10% fetal bovine serum (Gibco, MD, USA) (Dubleco's Modified Eagle Medium / Gibco, Were incubated at 37 ° C in a 5% CO ₂ incubator (Thermo Scientific, IL, USA) and subcultured at 2-day intervals.

For the measurement of NO production, RAW 264.7 cells were dispensed into 96-well plates at 2 × 10 ⁵ cells / well, and after 5 hours, the cells were treated with the above methods (whether or not there was hot water extraction, enzyme treatment, 8, 40, 200, 1000 ppm), Fig. 5b). The extracts of long-bred beetle larvae were treated with LPS and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. After the culture was completed, 100 μl of the culture was mixed with 100 μl of Griess reagent [1% (w / v) sulfanilamide in 5% (v / v) phosphoric acid and 0.1 (w / v) naphtylethylenediamine-HCl] plate for 10 minutes and then absorbance was measured at 540 nm. NaNO ₂ was used as a reference material.

NO is a free radical generated through enzyme catalysis of NOS (NO Synthase), which plays a pivotal role in regulating blood pressure and acting as a neurotransmitter in the immune response. Excess NO production is known to cause an inflammatory reaction.

Experimental Results As shown in FIGS. 5A and 5B, NO ₂ production in Raw cell 264.7 induced by LPS was statistically significantly inhibited, confirming the anti-inflammatory effect. Based on the cell viability analysis in Experimental Example 4 described later, it is considered that there is no significant effect on the cytotoxicity, and thus it can be used as a natural functional material.

Experimental Example  4: Cell survival analysis

Raw 264.7 cells were cultured in a medium containing 100 units / ml of penicillin-streptomycin and 10% fetal bovine serum (Gibco, MD, USA) (Dubleco's Modified Eagle Medium / Gibco, Were incubated at 37 ° C in a 5% CO ₂ incubator (Thermo Scientific, IL, USA) and subcultured at 2-day intervals.

To measure cell viability, RAW 264.7 cells were seeded in a 96 well plate at 2 × 10 ⁵ cells / well and treated with the extracts of the long-bred beetle larvae (8, 40, 200, 500, 1000 ppm) , And cultured in a 5% CO ₂ incubator for 24 hours.

The cells were incubated in a microplate reader (Beckman Coulter, CA, USA) at 37 ° C in a 5% CO ₂ incubator for 90 min. And the absorbance at 490 nm was measured to determine cell viability.

As a result, as shown in Fig. 6, the larvae scarab larvae extract showed cell survival rate of 88.03 ± 3.74 ~ 99.78 ± 5.13%, indicating that the anti-inflammatory activity at 8 to 1000 ppm concentration did not significantly affect the cell death .

Experimental Example  5: Depending on the type of enzyme and treatment conditions Hexanal ( hexanal ) Content measurement

Hexanal, which is one of the oxidation products, was analyzed by gas chromatogram to confirm that the degree of oxidation was decreased by reducing the odor and virgin components derived from insects and utilizing it as a food material.

Hexanal is a substance mainly produced by the oxidation of fatty acids such as oleic acid, linoleic acid and arachidonic acid and the decomposition of aldehydes, and is one of oxidation products. Is known as a component that emits. The fat content of the larvae of beetle larvae is 4.7% (oleic acid 44%, linoleic acid 4%).

As shown in FIG. 7, Hexanal was reduced by pretreatment (dietary control, fasting), extraction, centrifugation, and enzyme treatment, and it was confirmed that odor and vignetting decreased.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, As appropriate.

Claims (10)

1. And treating the long-lived beet larva with proteolytic enzyme.
2. The method according to claim 1,

   Wherein the long-bred beetle larva is cut for 1 to 120 hours and then pulverized.
3. The method according to claim 1,

   Wherein the proteolytic enzyme is at least one selected from the group consisting of pepsin, trypsin, flavorzyme, protamex, papain, alpha chymotrypsin and pancreatic acid.
4. The method according to any one of claims 1 to 3,

   Wherein the protease is treated at a concentration of 0.0001 to 5 parts by weight based on 100 parts by weight of the larval beetle larvae.
5. The method of claim 4,

   Wherein the protease is treated at a concentration of 0.01 part by weight to 3 parts by weight with respect to 100 parts by weight of the larval beetle larvae.
6. The method of claim 4,

   Wherein the proteolytic enzyme is treated for 5 minutes to 5 hours.
7. The method of claim 6,

   Wherein the proteolytic enzyme is treated for 30 minutes to 2 hours.
8. A method for manufacturing a semiconductor device, which is manufactured according to the manufacturing method of any one of claims 1 to 3,

   Longevity beetle larvae extract having a hexanal content of more than 0 mg / L and not more than 0.7 mg / L.
9. The method of claim 8,

   Wherein the content of L-tyrosine is from 0.1 mg / mL to 1.0 mg / mL.
10. A food comprising a long beetle larva extract of claim 1.

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