WO2019066485A1 - Procédé d'analyse de pureté pour vésicules extracellulaires, faisant appel à une chromatographie d'exclusion stérique - Google Patents

Procédé d'analyse de pureté pour vésicules extracellulaires, faisant appel à une chromatographie d'exclusion stérique Download PDF

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Publication number
WO2019066485A1
WO2019066485A1 PCT/KR2018/011415 KR2018011415W WO2019066485A1 WO 2019066485 A1 WO2019066485 A1 WO 2019066485A1 KR 2018011415 W KR2018011415 W KR 2018011415W WO 2019066485 A1 WO2019066485 A1 WO 2019066485A1
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WO
WIPO (PCT)
Prior art keywords
endoplasmic reticulum
extracellular endoplasmic
absorbance
extracellular
peak
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PCT/KR2018/011415
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English (en)
Korean (ko)
Inventor
고용송
이창진
박현택
Original Assignee
㈜로제타엑소좀
포항공과대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by ㈜로제타엑소좀, 포항공과대학교 산학협력단 filed Critical ㈜로제타엑소좀
Priority claimed from KR1020180114980A external-priority patent/KR102109921B1/ko
Publication of WO2019066485A1 publication Critical patent/WO2019066485A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86

Abstract

La présente invention concerne un procédé d'analyse de pureté pour vésicules extracellulaires, faisant appel à une chromatographie d'exclusion stérique, et plus particulièrement, la présente invention concerne un procédé d'utilisation de la capacité de séparation en fonction de la taille d'une chromatographie d'exclusion stérique et d'une spectroscopie, qui est unique ou combinant au moins deux types, afin de quantifier, dans divers échantillons, le volume de vésicules extracellulaires et divers contaminants, simultanément, et ainsi d'analyser la pureté, la quantité totale et le rendement de vésicules extracellulaires. Le procédé d'analyse de pureté pour vésicules extracellulaires, selon la présente invention, a l'avantage de pouvoir réaliser une analyse efficace tout en conservant la forme ou les propriétés des vésicules extracellulaires. De plus, le procédé d'analyse de pureté pour vésicules extracellulaires, selon la présente invention, permet de déterminer rapidement, simultanément, la pureté, la quantité totale et le rendement de vésicules extracellulaires qui ont été séparées à l'aide d'un procédé classique, et peut être utilisé, par exemple, dans le diagnostic de maladies, le traitement de maladies, la recherche multiomique et la recherche des propriétés de vésicules extracellulaires qui utilisent des vésicules extracellulaires séparées. En outre, le procédé d'analyse de pureté selon la présente invention peut également être appliqué au développement d'un nouveau procédé de séparation.
PCT/KR2018/011415 2017-09-27 2018-09-27 Procédé d'analyse de pureté pour vésicules extracellulaires, faisant appel à une chromatographie d'exclusion stérique WO2019066485A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20170124848 2017-09-27
KR10-2017-0124848 2017-09-27
KR10-2018-0114980 2018-09-27
KR1020180114980A KR102109921B1 (ko) 2017-09-27 2018-09-27 크기 배제 크로마토그래피를 이용한 세포밖 소포체의 순도 분석 방법

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WO2019066485A1 true WO2019066485A1 (fr) 2019-04-04

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113189067A (zh) * 2021-04-27 2021-07-30 浙江大学 一种细胞外囊泡的富集和检测方法及装置
CN117384741A (zh) * 2023-12-11 2024-01-12 上海晟燃生物科技有限公司 细胞外囊泡分离设备

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6002986A (en) * 1990-11-16 1999-12-14 Shimadzu Corporation Fraction purity measuring apparatus for chromatogram peak
KR20140139122A (ko) * 2012-03-30 2014-12-04 샹하이 테크웰 바이오파마슈티컬 컴퍼니, 리미티드 고순도 고리형 펩티드 화합물 및 그 제조방법과 용도

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6002986A (en) * 1990-11-16 1999-12-14 Shimadzu Corporation Fraction purity measuring apparatus for chromatogram peak
KR20140139122A (ko) * 2012-03-30 2014-12-04 샹하이 테크웰 바이오파마슈티컬 컴퍼니, 리미티드 고순도 고리형 펩티드 화합물 및 그 제조방법과 용도

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BOND, M. D.: "Evaluation of a dual-wavelength size exclusion HPLC method with improved sensitivity to detect protein aggregates and its use to better characterize degradation pathways of an IgG1 monoclonal antibody", JOURNAL *
CASTLEDINE, J. B. ET AL.: "A multiwavelength approach to the selection of absorbance ratios for the assessment of chromatographic peak purity", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 8 September 1991 (1991-09-08), pages 619 - 624, XP055586021 *
CASTLEDINE, J. B.: "Assessment of chromatographic peak purity by means of multi-wavelength detection and correlation-based algorithms", JOURNAL OF - CHROMATOGRAPHY, vol. 592, 1992, pages 27 - 36, XP026503508, DOI: doi:10.1016/0021-9673(92)85069-6 *
KOTMAKCI, M.: "Exosome isolation: Is there an optimal method with regard to diagnosis or treatment?", NOVEL IMPLICATIONS OF EXOSOMES IN DIAGNOSIS AND TREATMENT OF CANCER AND INFECTIOUS DISEASES, 12 July 2017 (2017-07-12), pages 163 - 182, XP055586020 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113189067A (zh) * 2021-04-27 2021-07-30 浙江大学 一种细胞外囊泡的富集和检测方法及装置
CN117384741A (zh) * 2023-12-11 2024-01-12 上海晟燃生物科技有限公司 细胞外囊泡分离设备
CN117384741B (zh) * 2023-12-11 2024-03-15 上海晟燃生物科技有限公司 细胞外囊泡分离设备

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