WO2019056166A1 - 生化反应装置及其套管机构 - Google Patents

生化反应装置及其套管机构 Download PDF

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Publication number
WO2019056166A1
WO2019056166A1 PCT/CN2017/102228 CN2017102228W WO2019056166A1 WO 2019056166 A1 WO2019056166 A1 WO 2019056166A1 CN 2017102228 W CN2017102228 W CN 2017102228W WO 2019056166 A1 WO2019056166 A1 WO 2019056166A1
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Prior art keywords
reaction
sleeve
hole
rod
reaction device
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PCT/CN2017/102228
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English (en)
French (fr)
Inventor
李俊明
林清格
蔡汮龙
李珮瑜
苏城
张晓芬
李馥君
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瑞基海洋生物科技股份有限公司
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Application filed by 瑞基海洋生物科技股份有限公司 filed Critical 瑞基海洋生物科技股份有限公司
Priority to CN201780090968.XA priority Critical patent/CN110650804A/zh
Priority to PCT/CN2017/102228 priority patent/WO2019056166A1/zh
Publication of WO2019056166A1 publication Critical patent/WO2019056166A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/04—Solvent extraction of solutions which are liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices

Definitions

  • the present disclosure relates to a biochemical reaction device, and more particularly to a cannula mechanism that facilitates replacement of a cannula and avoids operational contamination, and a biochemical reaction device including the cannula mechanism.
  • Biochemical reactions commonly used in biotechnology include nucleic acid extraction, polymerase chain reaction (PCR), nucleic acid complementary hybridization or immunological binding.
  • nucleic acids can be extracted from cells, viruses, bacteria or other biological tissues, and then the polymerase chain reaction can be used to amplify the target nucleic acid sequence specific or to be detected, and the amplified nucleic acid product can be Whether the nucleic acid complementary sequence of the nucleic acid sequence or its amplified nucleic acid product is present or hybridized by a nucleic acid complementary binding reaction with a primer linked to an identification marker during amplification or by hybridization of the product to a complementary nucleic acid sequence linked to the recognition marker How many.
  • an immune reaction with a specific binding property of the antibody can be utilized, and the identification mark attached thereto can also be used for qualitative or quantitative purposes.
  • biochemical reaction devices that can be automatically operated have been manufactured, such as automatic polymerase chain reaction devices, automatic nucleic acid extraction devices, and the like.
  • the biochemical reaction device performs an addition and a pipetting step of a reaction solution by an automatic mechanism such as a robot arm or a displacement mechanism, and adjusts reaction conditions such as temperature and reaction time by, for example, a temperature control component, thereby effectively reducing the burden on the operator. Improve the accuracy of the reaction.
  • an automatic mechanism such as a robot arm or a displacement mechanism
  • reaction conditions such as temperature and reaction time by, for example, a temperature control component
  • a reaction tube for the reaction is first installed, and the desired reaction solution is injected into the reaction tubes for subsequent nucleic acid amplification reaction, or by the reaction tubes.
  • Other extraction mechanisms such as a magnetic attraction mechanism perform nucleic acid extraction.
  • a plurality of parallel actuating mechanisms are usually provided, and the reaction tubes for the reaction are used.
  • the tube is connected by a plurality of reaction tubes, and in order to install the tube, a corresponding chute is required on the operation arm of the biochemical device, and the tube is inserted and slipped to the position.
  • the tube can achieve the purpose of simultaneously installing several reaction tubes, in the process of manual installation, the tube is often polluted and the subsequent reaction is affected. Similarly, after the reaction is completed, the tube must be manually removed or replaced, which may also cause problems in cleaning the interior of the device. Moreover, the pipe is usually connected with about eight or twelve reaction tubes. If the number of samples is not large, the tube of one unit must still be used, and the required number of reaction tubes cannot be installed according to requirements, so that the cost of consumables is relatively increased.
  • the purpose of the present disclosure is to provide a casing mechanism of a biochemical reaction device that does not need to manually install and extract a reaction tube, so that the reaction tube can be automatically installed in the biochemical reaction device, thereby avoiding the possibility of artificial installation.
  • the sample is contaminated.
  • Another object of the present disclosure is to provide a casing mechanism of a biochemical reaction device which can easily fit a reaction tube, through a special cassette structure corresponding to the reaction tube or a gap formed in the reaction tube, When the sleeve mechanism is actuated, the connecting member can be easily spliced with the reaction tube, and the position of the reaction tube can be positioned at the same time, and the mechanism of the related art is not easy to be detached or cannot be withdrawn when the tube is retracted. Disadvantages.
  • a further object of the present disclosure is to provide a casing mechanism for a biochemical reaction device that can be applied to different reaction tube specifications.
  • the detachable arrangement of the connector can be replaced with a corresponding connector according to the specifications of the reaction tube. It can be applied to the reaction of a variety of reaction tubes or reaction trays.
  • Another object of the present disclosure is to provide a casing mechanism for a biochemical reaction device in which a desired number of reaction tubes can be individually installed according to requirements, and it is not necessary to install a tube as in the related art, thereby reducing the reaction tube consumables. The cost required.
  • the present disclosure provides a casing mechanism of a biochemical reaction device, comprising: a first carrier, the first carrier is provided with at least one first perforation; at least one connector, each of the connections Corresponding to each of the first perforating devices, each of the connecting members is provided with a second perforation, and the second perforations are connected or overlapped with the first perforations, and each of the connecting members is juxtaposed a joint; and at least one sleeve (reaction tube) having opposite ends, wherein one end is an open nozzle end, each of the sleeves being separable from the nozzle end to the connector The joints are connected.
  • the casing mechanism of the biochemical reaction device wherein the sleeve may be provided with a latch portion, which may correspond to the connector when the sleeve is connected The joint is snapped.
  • the engaging portion is an annular protruding structure provided on an outer edge of the connecting member, and the latching portion is a corresponding groove structure, but is not limited thereto.
  • the engaging portion and the latch portion may also be interchanged with the foregoing structure, that is, the engaging portion may be a groove structure provided on an outer edge of the connecting member, and the latch portion is Corresponding annular protruding structures; or other structures that can be temporarily connected and then separated, and have correspondingly engaged, tightly fitted, and spliced.
  • the casing mechanism of the biochemical reaction device wherein each of the connecting members is detachably mounted under the first carrier corresponding to each of the first through holes
  • the second through hole of the connecting member is in communication with the first through hole.
  • each of the connecting members may be detachably mounted in the first through hole, and the engaging portion is exposed under the first carrying member.
  • the second through hole of the connecting member overlaps with the first through hole.
  • the connectors may be replaced with correspondingly connectable structures depending on the size of the sleeve to be connected.
  • the casing mechanism of the biochemical reaction device wherein the first carrier member is further provided with a cover plate to limit the connecting members, and the cover plate is An insertion hole corresponding to the first through hole and communicating therewith may be disposed.
  • the casing mechanism of the biochemical reaction device wherein the sleeve is further provided with a rib at an outer edge below the click portion to enable the sleeves It can be pre-placed on a casing or reaction disk, which is then automatically attached to the connector by the casing mechanism of the disclosed embodiment.
  • the sleeve is provided with at least one gap from the end edge of the nozzle end, and the gap may be opened in the axial direction of the sleeve, but is not limited thereto.
  • a biochemical reaction device for reacting a solution in a reaction tray, comprising: a sleeve mechanism as described above and a magnetic attraction mechanism, the magnetic attraction
  • the mechanism includes a second carrier, the second carrier is provided with at least one reaction rod, and the reaction rod is disposed corresponding to the first perforation; wherein the sleeve mechanism and the magnetic mechanism are respectively connected a longitudinal displacement mechanism, when the magnetic attraction mechanism is longitudinally displaced downward, the plurality of reaction rods provided thereon are A perforation extends into the sleeve and moves a tip of the reaction rod to a predetermined position within the sleeve.
  • the magnetic attraction mechanism can be actuated together with the sleeve mechanism to make the sleeve And the reaction rod disposed therein is moved into or out of at least one of the holes provided in the reaction tray to perform the reaction.
  • the biochemical reaction device wherein the reaction rod can generate a magnetic rod, which can be a permanent magnet or an electromagnet rod, but is not limited thereto.
  • the casing can be automatically connected by the casing mechanism after being prepared according to the required number, which not only improves the cost of the casing, but also avoids labor.
  • the pollution problem caused by the operation can be easily sleeved on the connecting member, and the positioning and the easy retraction are achieved.
  • the connection can be separated and disassembled by the connecting member, and the present disclosure can also be applied to the bushings of different specifications.
  • FIG. 1 is a schematic block diagram of an embodiment of a biochemical reaction device of the present disclosure including an embodiment of a cannula mechanism.
  • FIG. 2 is a perspective view and a partial enlarged view of an embodiment of the sleeve mechanism of the present disclosure.
  • FIG 3 is a perspective view of another embodiment of the embodiment of the sleeve mechanism of the present disclosure.
  • Figure 4 is a schematic illustration of the embodiment of the sleeve mechanism of the present disclosure and the embodiment of the magnetic mechanism.
  • Figure 5 is a side elevational view of the embodiment of the sleeve mechanism of the present disclosure and the embodiment of the magnetic mechanism.
  • Figure 6 is a schematic illustration of an embodiment of a magnetic attraction mechanism of the present disclosure with an embodiment of a reaction rod inserted into a cannula mechanism.
  • FIG. 1 is a schematic diagram of an assembly of an embodiment of a biochemical reaction device including an embodiment of a casing mechanism.
  • the biochemical reaction device provided by the embodiment of the present disclosure comprises: a casing mechanism 10, a magnetic attraction mechanism 20, a reaction disk 30, a base 40 and a displacement mechanism 50.
  • the casing mechanism 10 and the magnetic attraction mechanism 20 are respectively connected
  • a longitudinal displacement mechanism (not shown) disposed on the base 40 is vertically displaceable relative to the base 40.
  • the reaction disk 30 can be laterally displaced laterally on the bottom platform of the base 40 by a lateral displacement mechanism 50.
  • the casing 13 as the reaction tube can first be connected to the casing mechanism 10 under automatic operation, and then the mechanism of interaction between the casing mechanism 10 and the magnetic attraction mechanism 20 is coordinated.
  • the sleeve 13 is automatically moved into or out of the hole 31 provided in the reaction disk 30 as set.
  • an automatic nucleic acid extraction device is taken as an example, which uses magnetic beads and a magnetic attraction principle to perform nucleic acid extraction. Therefore, the magnetic mechanism 20 is provided to operate, and if a reaction solution is simply added to the cannula, for example, nucleic acid amplification is performed. When the hybridization or hybridization reaction is carried out, the magnetic attraction mechanism 20 may not be provided.
  • FIG. 2 and FIG. 3 are perspective views of the embodiment of the sleeve mechanism of the present disclosure.
  • the casing mechanism 10 includes a first carrier 11, a connector 12 and a sleeve 13.
  • the connecting member 12 is mounted on the first carrier 11 and is connected to the sleeve 13.
  • a plurality of first through holes 111 are defined in the first carrier member 11.
  • the number of the first through holes 111 can be correspondingly set according to the number of holes in a column of a common reaction disk.
  • the first carrier 11 can be connected to a first extension member 14 and connected to a longitudinal displacement mechanism (not shown) through the first connection portion 15 provided therein, or directly connected through the first connection portion 15 provided.
  • the first connecting portion 15 only connects and fixes the first carrier 11 to the longitudinal displacement mechanism, and is not particularly limited. It can be detachably connected by screws or connected by welding, snapping, or the like.
  • the connecting member 12 can be directly disposed under the first carrying member 11 corresponding to the first through hole 111, or can be disposed in the first through hole 111 by a stepped groove (not shown) by using a splicing method.
  • the connecting members 12 are respectively inserted into the first through holes 111 to be connected thereto.
  • Each of the connecting members 12 is respectively provided with a second through hole 123. If the connecting member 12 is mounted under the first carrying member 11, the second through hole 123 penetrates through the first through hole 111 and can communicate with each other.
  • the second through hole 123 is disposed in the first through hole 111 and extends outside the first through hole 111.
  • the connecting member 12 is mounted in a splicing manner.
  • a cover plate 112 may be further disposed on the first carrying member 11 and the cover plate 112 is mounted thereon.
  • the insertion hole 113 corresponding to the first through hole 111 / the second through hole 123 is opened, and the insertion hole 113 is in communication with the second through hole 123 .
  • the connecting member 12 is provided with a joint portion 121 and a base portion 122 on the outer edge surface. Regardless of whether the connecting member 12 is mounted on the first through hole 11 or the first through hole 111 that is connected thereto, the engaging portion 121 needs to be exposed below the first carrying member 11 to be connected with the sleeve 13 .
  • the joint portion 121 is a ring-shaped protruding structure in the embodiment, but is not limited thereto. It may also be an annular distribution of the circular convex structure, or an annular groove and a circular concave structure. Or a snap structure or the like corresponding to the latch portion 132.
  • the base portion 122 is disposed above the joint portion 121 and between the joint portion 121 and the first carrier member 11 as a limiting structure for the end of the sleeve 13 to be abutted after being connected to the sleeve 13.
  • the sleeve 13 that is, the reaction tube for carrying out the reaction, can be used as a test tube in which the reaction solution is accommodated, or as a mechanism for actuating the reaction as the magnetic attraction mechanism 20 as in the present embodiment.
  • the sleeve 13 is open at one end, and the opposite end is a closed tubular structure. The opposite end can be opened as required, and is not limited thereto; the length of the tubular body
  • the size of the pipe diameter can be selected according to the requirements of the reaction volume and the specifications of the hole 31 provided in the reaction disk 30.
  • One end of the sleeve 13 is open at the nozzle end 131, and the inner edge of the sleeve 13 near the nozzle end 131 is provided with a latching portion 132.
  • the latching portion 132 can be connected to the engaging portion 121 provided thereto. Therefore, the latching portions 132 are connected to each other corresponding to the engaging portion 121, and may be a groove, an annular groove structure, or a circular protrusion, a bump, a ring protruding structure, or a joint portion. 121 corresponding snap structure and the like.
  • the sleeve 13 is at the open end 131.
  • the opening 13 of the sleeve 13 can be opened by the open end 131 with at least one gap 1311 shaped like a groove but not limited thereto, so that the connecting member 12 is open to the open end 131.
  • the open end 131 can be slightly extended due to the setting of the gap 1311, so that the connecting member 12 can be easily inserted into the sleeve 13 through the open end 131.
  • the gap 1311 can be opened parallel to the axial direction of the sleeve 13, but is not limited thereto, and the number of the gap 1311 is not particularly limited, and the setting can be adjusted according to the diameter of the pipe or the hardness of the casing material. .
  • a rib 133 may be further disposed on the outer edge of the sleeve 13 below the latching portion 132 for placing the sleeve 13 on the reaction plate 30 or the mounting hole (not shown) of the casing frame, and then The connection is made with the casing mechanism 10.
  • the ribs 133 may be annularly arranged or provided in plurality and arranged in an annular shape, and the shape thereof is not particularly limited.
  • the reaction disk 30 or the casing frame in which the required number of casings is packaged may be prepared (in which the casing 13 has been first arranged through the ribs 133 thereof and placed in the orifice where the reaction disk 30 is not reacted. 31, or the placement hole of the casing frame, and then sealed (packed)), after being torn and packaged, placed on the lateral displacement mechanism 50, while operating the lateral displacement mechanism 50 and the longitudinal direction of the casing mechanism 10
  • the displacement mechanism displaces the connecting member 12 of the sleeve mechanism 10 above the nozzle end 131 of the sleeve 13 and further displaces downward to the top.
  • the joint portion 121 on the connecting member 12 is engaged with the latch portion 132 of the sleeve 13, and the subsequent reaction operation can be performed after the hooking.
  • the end of the sleeve end 13 of the sleeve 13 may be plastic or other material having slightly deformed characteristics, and the connecting member 12 may be abutted when the connecting member 12 is abutted. Slightly deformed, the connector 12 can be further inserted into the sleeve 13 to connect the engaging portion 121 to the latch portion 132.
  • the automatic nucleic acid extraction device utilizing the magnetic attraction reaction is to add modified magnetic microbeads or magnetically attractable microbeads to the sample treated wells, and the modified microbeads can adhere to the sample by the sample.
  • the nucleic acid released after the reaction is passed through a cannula, and a magnetic rod is placed in the sleeve, and the two are immersed in the solution with the microbead added thereto, and the nucleic acid is adhered by magnetic attraction.
  • the beads are adsorbed on the outer wall of the sleeve, and then the sleeve with the beads adsorbed is moved to another column or array of wells for cleaning reaction, and the impurities other than the nucleic acid are washed and removed, and then moved to In another row of wells, the beads are separated from the nucleic acid to obtain purified nucleic acid, and the beads adsorbed on the sleeve can be recycled and reused after being removed from the magnetic rod.
  • FIG. 4 and FIG. 5 are schematic diagrams of the embodiment of the casing mechanism and the magnetic mechanism of the present disclosure.
  • the biochemical reaction device of the present disclosure further includes a magnetic attraction mechanism 20 including: a second carrier 21 and a reaction rod 22.
  • the second carrier member 21, which in the present embodiment is a long plate body/block, is not particularly limited in its structure.
  • the second carrier 21 is provided with a plurality of reaction rods 22 below it. The number and position of the reaction rods 22 correspond to the insertion holes 113 (or the first perforations 111 / the second perforations 123) in the casing mechanism 10.
  • the second carrier 21 can be connected to a second extension member 23 and connected to a longitudinal displacement mechanism (not shown) through the second connection portion 24 provided thereto, or directly connected through the provided second connection portion 24.
  • the second connecting portion 24 only connects and fixes the second carrier 21 to the longitudinal displacement mechanism, and is not particularly limited. It can be detachably connected by screws or connected by welding, jamming, or the like.
  • the reaction rod 22 is a magnetically vibrating rod which can be a permanent magnet or an electromagnet type rod.
  • FIG. 6 is a schematic diagram of an embodiment of the magnetic attraction mechanism of the present disclosure in which the reaction rod is inserted into the sleeve mechanism.
  • the sleeve mechanism 10 and the magnetic attraction mechanism 20 are respectively connected to a longitudinal displacement mechanism (not shown), which can be operated individually or simultaneously according to the setting.
  • the magnetic attraction mechanism 20 is displaced downward, so that the reaction rod 22 is inserted into the operating cavity 130 provided by the sleeve 13 by the insertion hole 113 (or the first perforation 111 / the second perforation 123).
  • the predetermined position may be selected according to the configuration of the top end 221 and the configuration of the bottom portion 134 of the actuation chamber 130, and is not particularly limited, and is generally close to the bottom 134 but not yet touched.
  • the predetermined position is preferred to provide a better magnetic attraction for the bottom of the sleeve 13 on the one hand, and to prevent the sleeve 13 from being loosened by the connecting member 12 due to the contact of the reaction rod 22 on the one hand.
  • the magnetic attraction mechanism 20 is actuated together with the sleeve mechanism 10, and cooperates with the lateral displacement mechanism 50 to insert the sleeve 13 together
  • the reaction rod 22 is displaced together with the column hole 31 to be magnetized (please refer to FIG. 1 at the same time), and then moved together into the column hole groove 31 to adhere the nucleic acid to the solution.
  • the beads are adsorbed on the outer wall of the sleeve 13 and then moved up the column aperture 31 together.
  • the reaction disk 30 After moving up, the reaction disk 30 is displaced by a series of holes by the lateral displacement mechanism 50, so that the sleeve 13/reaction rod 22 is located above the next column of holes, and then moved down together into the hole 31 of the next column for cleaning.
  • the cleaning reaction can be set with different number of cleaning liquids as required, and the number of cleaning steps of the number of columns is repeated, and finally, the pores are filled into a solution containing a solution capable of separating the nucleic acid from the microbeads, and the nucleic acid is The purified nucleic acid can be recovered after separation of the beads.
  • the sleeve 13 can be automatically and easily connected to the connecting member 12 by the device operation in the initial step, thereby avoiding the manual installation of the sleeve. 13, which easily causes the contamination of the outer edge of the tube wall of the cannula 13, so that the nucleic acid extraction procedure greatly reduces the intervention of foreign sample contamination, and the subsequent reaction reaction will make the reaction result more accurate or reduce the false positive. produce.
  • the corresponding number of sleeves can be prepared according to requirements, so that the cost of consumables can also be reduced.

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Abstract

一种生化反应装置及其套管机构(10)，套管机构(10)包括：第一载件(11)，第一载件(11)上设有至少一个第一穿孔(111)；至少一个连接件(12)，各连接件(12)是对应于各第一穿孔(111)装设，各连接件(12)设有第二穿孔(123)，第二穿孔(123)与第一穿孔(111)是相贯穿连通或相重迭，且各连接件(12)并设有一接合部(121)；以及至少一个套管(13)，其具有相对两端，其中一端为开放的管口端(131)，各套管可由管口端(131)以可分离方式与连接件(12)的接合部(121)相连接。

Description

生化反应装置及其套管机构 技术领域
本公开关于一种生化反应装置，尤其是关于一种可方便更换套管并避免操作污染的套管机构，及包含该套管机构的生化反应装置。
背景技术
一般常见利用于生物技术的生化反应包括了核酸萃取、聚合酶链锁反应(PCR)、核酸互补杂交反应或是免疫结合反应。利用所述些技术与反应，可由细胞、病毒、细菌或其他生物组织中抽取出核酸，之后再利用聚合酶链锁反应扩增其中特定或待检测的标的核酸序列，扩增的核酸产物可于扩增过程中以连接有辨识标记的引子进行核酸互补结合反应，或将产物与连接有辨识标记的互补核酸序列进行杂交反应，即可确认所述标的核酸序列或其扩增核酸产物是否存在或其多寡。此外，对于蛋白质或寡胜肽的确认，则可利用抗体具专一结合特性的免疫反应，同样通过其所连接的辨识标记，达到定性或定量的目的。
然而，操作所述些反应时，若需处理的样本数较多，操作人员将重复地在各样本间加入或移出反应溶液或产物，不但负担大，更容易在操作过程中弄错样本、加错、多加或少加某一溶液，或因操作步骤多而造成样本的污染，使得反应溶液产生了操作上的误差，而影响反应结果的准确性。
因此，为克服并减少所述些操作上的误差，许多可自动进行操作的生化反应装置已被制造应用，例如自动聚合酶链锁反应装置、自动核酸萃取装置等。所述些生化反应装置，通过机械臂或位移机构等自动机构进行反应溶液的添加、移液步骤，并以例如温控组件调整温度、反应时间等反应条件，着实降低了操作人员的负担，也提高了反应的准确性。然而，即便是自动化装置，目前尚无法将每一步骤自动化，在部分步骤中仍需人工操作的介入。例如：进行反应操作前，需先装设反应用的反应管，用于于所述些反应管中注入所需的反应溶液以进行后续的核酸扩增反应，或是通过所述些反应管配合其他例如磁吸机构来进行核酸的萃取反应。在一般前述自动化的反应装置中，为能多样本一同操作与反应，通常设有数个并列的作动机构，而利用于所述所述反应的反应管， 一般是以数反应管相连接的排管为的，且为装设所述排管，生化装置的操作臂上需开设以相对应的滑槽，供所述排管插设并滑移至定位
虽然排管的设置可达到数反应管同时装设的目的，但在人工装设的过程中，却常产生排管受有污染而影响到后续反应的情形。同样的，反应完毕后也须以人工将排管拔出或替换，也容易造成装置内部清洁上的问题。尚且，排管通常连接有约八或十二个反应管，若样本数不甚多时，仍须使用一个单元的排管而无法依需求装设所需的反应管数目，使得耗材成本相对提高。
发明内容
本公开目的在于提供一种无须以人工进行反应管装设与拔出的生化反应装置的套管机构，使反应管得于生化反应装置内自动进行装设，而避免因人工装设所容易产生样本受污染的问题。
本公开的另一目的在于提供一种可轻易套接反应管的生化反应装置的套管机构，通过连接件与反应管相对应的特殊卡掣结构或是/以及反应管上所开设的间隙，于所述套管机构作动时能使连接件轻易地与反应管相套接，不但能同时定位反应管套接位置，并避免了相关技术中的机构退管时不易脱离或无法退管的缺点。
本公开的再一目的则在于提供一种可适用不同反应管规格的生化反应装置的套管机构，通过连接件可分离的设置方式，可依反应管的规格更换以相对应的连接件，因此可应用于多种反应管或反应盘的反应中。
本公开的另一目的则在于提供一种可依需求个别装设所欲数目反应管的生化反应装置的套管机构，无须如相关技术中仅得装设以排管，因而可降低反应管耗材所需的成本。
为了达成前述的目的，本公开提供一种生化反应装置的套管机构，包括：第一载件，所述第一载件上设有至少一个第一穿孔；至少一个连接件，各所述连接件对应于各所述第一穿孔装设，各所述连接件设有第二穿孔，所述第二穿孔与所述第一穿孔相贯穿连通或相重迭，且各所述连接件并设有接合部；以及至少一个套管(反应管)，其具有相对两端，其中一端为开放的管口端，各所述套管可由所述管口端以可分离方式与所述连接件的所述接合部相连接。
在本公开的一实施例中，所述的生化反应装置的套管机构，其中所述套管可设有卡掣部，其于所述套管连接时可与所述连接件上相对应的所述接合部相卡接。在本公开实施例中，所述接合部系于所述连接件外缘所设的环状突出结构，而所述卡掣部系相对应的凹槽结构，但并不以此为限。所述接合部与所述卡掣部也可为前述结构的互换，也就是说，所述接合部可为所述连接件外缘所设的凹槽结构，而所述卡掣部则是相对应的环状突出结构；或其他可以暂时连接再加以分离，而具有相对应卡合、紧配、崁接的结构。
在本公开的一实施例中，所述的生化反应装置的套管机构，其中各所述连接件可对应于各所述第一穿孔以可分离方式装设于所述第一载件的下方，所述连接件的所述第二穿孔与所述第一穿孔相贯穿连通。
在本公开实施例中，各所述连接件也可以可分离方式崁接装设于所述第一穿孔中，并使所述接合部外露于所述第一载件的下方，此时，所述连接件的所述第二穿孔与所述第一穿孔相重迭。
在本公开实施例中，所述些连接件并可依所连接的套管规格更换以相对应而可连接的结构。
在本公开的一实施例中，所述的生化反应装置的套管机构，其中所述第一载件上可进一步装设有一盖板，藉以限位所述些连接件，所述盖板并可设有与所述第一穿孔相对应且相贯穿连通的一插设孔。
在本公开的一实施例中，所述的生化反应装置的套管机构，其中所述套管于所述卡掣部下方的外缘处可进一步设有一凸肋，以使所述些套管可预先置放于一套管架或反应盘上，再由本公开实施例的套管机构自动将其连接至连接件上。
在本公开实施例中，所述套管由所述管口端的端缘开设有至少一个间隙，所述间隙可近平行于所述套管轴向方向开设，但并不以此为限。
在本公开的另一实施例中，提供一种生化反应装置，用于使一反应盘内的溶液进行反应，包括：一如前所述的套管机构以及一磁吸机构，所述磁吸机构包括一第二载件，所述第二载件上装设至少一个反应杆，所述反应杆对应于所述第一穿孔设置；其中，所述套管机构与所述磁吸机构分别连接有一纵向位移机构，当所述磁吸机构纵向向下位移时，其所设的所述些反应杆可经由所述第 一穿孔穿伸进入所述套管中，并使所述反应杆的一顶端移动至所述套管内的一预定位置。
在本公开的一实施例中，所述的生化反应装置，其中所述反应杆移动至所述预定位置后，所述磁吸机构可与所述套管机构一同作动，使所述套管以及穿设其中的所述反应杆，一同移入或移出所述反应盘所设的至少一个孔槽中以进行反应。
在本公开的一实施例中，所述的生化反应装置，其中所述反应杆可产生磁性的杆体，其可为永久磁铁或电磁铁的杆体，但并不以此为限。
通过本公开所提供的套管机构与生化反应装置，套管可依所需的数量备置后，由套管机构自动地进行连接，不但可改善套管使用的成本问题，更可避免因为人工操作所产生的污染问题。此外，通过本公开，更可使套管轻易的套接在连接件上，并达到定位以及容易退管的目的。同时，通过连接件可分离拆换的设置方式，也使本公开可应用于不同规格的套管上。
以下将进一步说明本公开的实施方式，下述所列举的实施例用于阐明本公开，并非用于限定本公开的范围，任何熟习此技艺者，在不脱离本公开的精神和范围内，当可做些许更动与润饰，因此本公开的保护范围当视后附的申请专利范围所界定者为准。
附图说明
图1是本公开包括套管机构实施例的生化反应装置实施例的组立示意图。
图2是本公开套管机构实施例的立体示意图以及局部放大图。
图3是本公开套管机构实施例另一角度的立体示意图。
图4是本公开套管机构实施例与磁吸机构实施例作动的示意图。
图5是本公开套管机构实施例与磁吸机构实施例作动的侧视示意图。
图6是本公开磁吸机构实施例以反应杆插入套管机构实施例的示意图。
具体实施方式
请参阅图1，所述图是本公开包括套管机构实施例的生化反应装置实施例的组立示意图。本公开实施例所提供的生化反应装置，包括：套管机构10、磁吸机构20、反应盘30、基座40与位移机构50。套管机构10与磁吸机构20分别连 接于一设于基座40上的纵向位移机构(图中未示)上，可相对于基座40进行上下的纵向位移。反应盘30则可利用一横向位移机构50于基座40的底部平台上进行水平方向的横向位移。通过套管机构10的作动，作为反应管的套管13首先可在自动化操作下连接至套管机构10上，的后再透过套管机构10与磁吸机构20相互配合的作动机制，使套管13依设定自动地移入或移出反应盘30所设的孔槽31。本实施例中，是以自动核酸萃取装置为例，其利用磁珠与磁吸原理进行核酸的萃取，因此设置以磁吸机构20进行作动，若单纯套管添加反应溶液以进行例如核酸扩增或杂交反应时，则可不设置以磁吸机构20。
请再同时参阅图2与图3，图2与图3是本公开套管机构实施例的立体示意图。套管机构10，包括第一载件11、连接件12与套管13。连接件12装设于第一载件11上，并再与套管13相连接。
第一载件11，本实施例中是一长型板体，但其结构并未设有特别的限制。第一载件11上开设有多个第一穿孔111，第一穿孔111的数目可依一般常见的反应盘所设一列的孔槽数目相应设置。第一载件11可连接一第一延伸件14，并通过其所设的第一连接部15连接至一纵向位移机构(图中未示)，或直接通过所设的第一连接部15连接至所述纵向位移机构。第一连接部15仅将第一载件11连接固定于纵向位移机构，并未设有特别的限制，可利用螺丝以可拆卸的方式连接，或以焊接、卡接后粘连等方式进行连接。
连接件12，可直接对应第一穿孔111装设于第一载件11的下方，或是利用崁接方式，于第一穿孔111中先设置以阶梯状崁槽(图中未示)后，将连接件12分别穿入各所述第一穿孔111而崁接其中。各连接件12分别设有一第二穿孔123，若连接件12装设于第一载件11的下方，则第二穿孔123与第一穿孔111相贯穿并可相互连通，若连接件12以崁接方式装设于第一穿孔111中，此时，第二穿孔123重迭设置于第一穿孔111中并延伸至第一穿孔111外。于本实施例中，连接件12以崁接方式装设，为将连接件12限位于第一穿孔111中，可于第一载件11上进一步装设一盖板112，盖板112上并开设有与第一穿孔111/第二穿孔123相对应的插设孔113，且插设孔113与第二穿孔123相贯穿连通。连接件12装设于第一载件11上时可以可分离的方式组设，例如卡接、锁螺、崁接等，并未设有特别的限制。
连接件12于外缘表面则设有一接合部121与一基部122。连接件12无论装设于第一载件11下方或崁接于其中的第一穿孔111，所述接合部121皆需外露于第一载件11的下方，以与套管13相连接。接合部121于本实施例中是一环状突出结构，但并不以此为限，其也可为圆凸结构环状分布设置，或环状凹槽、圆形凹陷结构环状分布设置，或是与卡掣部132相对应的卡合结构等。基部122设于接合部121上方，且位于接合部121与第一载件11之间，可作为与套管13连接后，套管13末端抵顶的限位结构。
套管13，也就是进行反应的反应管，可作为反应溶液容置的试管，或如本实施例是作为磁吸机构20作动反应的机构的一。套管13于本实施例中是一端为开放，相对一端为封闭的管体结构，其也可依需求，使所述相对一端呈开放，故并不以此为限；所述管体的长短、管径大小，可依反应体积的需求以及反应盘30所设孔槽31的规格选择设置。套管13开放的一端为管口端131，接近管口端131的套管13内缘则设有一卡掣部132。所述卡掣部132于套管13连接于连接件12时，恰可与其所设的接合部121相连接。因此，卡掣部132是对应于所述接合部121而可相互连接的结构，其可为凹槽、环状凹槽结构，或圆凸、凸块、环状突出结构，或是与接合部121相对应的卡合结构等。套管13于所述开口端131，可如本实施例由所述开口端131开设以至少一个形状类似沟槽但并不以此为限的间隙1311，使所述连接件12往开口端131抵顶套接时，能因间隙1311的设置，使开口端131稍微撑开，让连接件12更容易穿越开口端131而套入套管13中。间隙1311可以接近平行于所述套管13轴向方向开设，但并不以此为限，而其所设数目也未设有特别的限制，可依据管径大小或套管材质的硬度调整设置。套管13于卡掣部132下方的外缘处可进一步设有一凸肋133，用于将套管13置放于反应盘30或套管架的置放孔(图中未示)上，再以套管机构10进行连接。凸肋133可环状设置，或设有多个而以环状分布设置，而其形状并未设有特别的限制。
进行套管13连接时，可先准备包装好所需套管数目的反应盘30或套管架(其中套管13已先通过其凸肋133排列置放于反应盘30未进行反应的孔槽31上，或套管架的置放孔上，再予密封包装)，撕开包装后将其置放于横向位移机构50上，同时操作横向位移机构50与连接所述套管机构10的纵向位移机构，使套管机构10的连接件12位移至套管13管口端131上方后，进一步向下位移抵顶， 使连接件12上的接合部121与套管13的卡掣部132进行卡接，卡接后即可进行后续的反应作动。当套管13是以套接方式套接于连接件12上时，套管13其管口端131所述端可为塑料或其他稍具变形特性的材质，而可于连接件12抵顶时稍微形变，使连接件12能进一步往套管13内伸入，让接合部121与卡掣部132进行连接。
所谓利用磁吸反应的自动核酸萃取装置，是在样本经处理的孔槽中加入经改质的磁性微珠或可受磁力吸引的微珠，所述些改质的微珠能够黏附由样本经反应后所释放出的核酸，的后再通过一套管，并于所述套管中装设有一磁性棒，二者一同沉浸于前述添加有微珠的溶液中，通过磁吸力将黏附有核酸的微珠吸附在套管外缘管壁上，而后再将吸附有微珠的套管移动至另一列或数列的孔槽中进行清洗反应，将核酸以外的杂质清洗移除后，再移动至另一列孔槽中将微珠与核酸分离，即可获得纯化的核酸，而吸附在套管上的微珠则可于移出磁性棒后回收再次利用。
请同时参阅图4与图5并配合图1，图4与图5是本公开套管机构实施例与磁吸机构实施例作动的示意图。为达成前述通过磁吸作用完成核酸萃取反应，本公开的生化反应装置进一步设有一磁吸机构20，其包括：第二载件21与反应杆22。第二载件21，于本实施例中是一长型板体/块体，但其结构并未设有特别的限制。第二载件21于其下方装设有多个反应杆22。反应杆22所设数目与位置是对应于套管机构10中的插设孔113(或第一穿孔111/第二穿孔123)。第二载件21可连接一第二延伸件23，并通过其所设的第二连接部24连接至一纵向位移机构(图中未示)，或直接通过所设的第二连接部24连接至所述纵向位移机构。第二连接部24仅是将第二载件21连接固定于纵向位移机构，并未设有特别的限制，可利用螺丝以可拆卸的方式连接，或以焊接、卡接后粘连等方式连接。反应杆22于本实施例中是一可产生磁性的杆体，其可为永久磁铁或电磁铁型式的杆体。
请再同时参阅图6，所述图是本公开磁吸机构实施例以反应杆插入套管机构实施例的示意图。套管机构10与与磁吸机构20是分别连接于一纵向位移机构(图中未示)，可依照设定个别或同时作动。于进行磁吸作用前，磁吸机构20向下位移，使反应杆22由插设孔113(或第一穿孔111/第二穿孔123)穿伸进入套管13所设的作动腔130中，并使反应杆22端部的顶端221移动至作动腔130内所设 的预定位置，所述预定位置可依据顶端221的构型以及作动腔130的底部134的构型而加以选择设置，并未设有特别的限制，一般是以接近底部134但尚未碰触的预定位置为佳，一方面可为套管13底部提供较佳的磁吸力，一方面则可避免因反应杆22的碰触而使套管13由连接件12松脱。
当磁吸机构20的反应杆22位移至预定位置后，所述磁吸机构20则与所述套管机构10一同作动，配合横向位移机构50，将所述套管13连同穿设其中的反应杆22，一同位移至所欲进行磁吸作用的所述列孔槽31上方后(请同时参见图1)，再一同下移进入所述列孔槽31中，将溶液中黏附有核酸的微珠吸附于套管13的外缘管壁上，而后一同上移离开所述列孔槽31。上移后，反应盘30经横向位移机构50位移一列孔槽距离，使套管13/反应杆22位于下一列孔槽的上方后，又再一同下移进入下一列的孔槽31中进行清洗反应，此清洗反应可依需求设置以不同列数的清洗液，而重复所述列数次数的清洗步骤，最后则是进入含有能将核酸与微珠分离的溶液的孔槽中，将核酸与微珠分离后即可回收获得纯化的核酸。
通过上述套管机构10，以及配合磁吸机构20的作动，能够在初始步骤中自动地、轻易地由装置操作将套管13连接至连接件12上，着实避免了因人工装设套管13，而容易造成套管13管壁外缘受污染的问题，使核酸萃取程序大幅减少外来样本污染的介入，则于后续进行PCR反应时，将使其反应结果更为准确或减少伪阳性的产生。另一方面，若所述次欲进行试验或反应的样本数较少时，则可依需求备置相对应的套管数目，因此也能达到降低耗材成本的目的。

Claims (10)

  1. 一种生化反应装置的套管机构，包括：
    第一载件，所述第一载件上设有至少一个第一穿孔；
    至少一个连接件，各所述连接件是对应于各所述第一穿孔装设，各所述连接件设有一第二穿孔，所述第二穿孔与所述第一穿孔是相贯穿连通或相重迭，且各所述连接件并设有接合部；以及
    至少一个套管，具有相对两端，其中一端为开放的管口端，各所述套管并设有卡掣部，可由所述管口端以可分离方式与所述连接件上相对应的所述接合部相卡接；
    其中，所述接合部是于所述连接件外缘所设的环状突出结构，而所述卡掣部是相对应的凹槽结构，或所述接合部是于所述连接件外缘所设的凹槽结构，而所述卡掣部是相对应的环状突出结构。
  2. 根据权利要求1所述的生化反应装置的套管机构，其中各所述连接件是对应于各所述第一穿孔以可分离方式装设于所述第一载件的下方，所述连接件的所述第二穿孔与所述第一穿孔是相贯穿连通。
  3. 根据权利要求1所述的生化反应装置的套管机构，其中各所述连接件是以可分离方式崁接装设于所述第一穿孔中，并使所述接合部外露于所述第一载件的下方，所述连接件的所述第二穿孔与所述第一穿孔是相重迭。
  4. 根据权利要求3所述的生化反应装置的套管机构，其中所述第一载件上进一步装设有盖板，藉以限位所述些连接件，所述盖板并设有与所述第一穿孔相对应且相贯穿连通的一插设孔。
  5. 根据权利要求1所述的生化反应装置的套管机构，其中所述套管于所述卡掣部下方的外缘处进一步设有凸肋。
  6. 根据权利要求1所述的生化反应装置的套管机构，其中所述套管在所述管口端的端缘开设有至少一个间隙。
  7. 一种生化反应装置，用于使一反应盘内的溶液进行反应，包括：
    根据权利要求1至6任一项的套管机构；以及
    磁吸机构，所述磁吸机构包括第二载件，所述第二载件上装设至少一个反应杆，所述反应杆是对应于所述第一穿孔设置；
    其中，所述套管机构与所述磁吸机构分别连接有纵向位移机构，当所述磁吸机构纵向向下位移时，其所设的所述些反应杆可经由所述第一穿孔穿伸进入所述套管中，并使所述反应杆的顶端移动至所述套管内的预定位置。
  8. 根据权利要求7所述的生化反应装置，其中所述反应杆移动至所述预定位置后，所述磁吸机构可与所述套管机构一同作动，使所述套管以及穿设其中的所述反应杆，一同移入或移出所述反应盘所设的至少一个孔槽以进行反应。
  9. 根据权利要求7或8所述的生化反应装置，其中所述反应杆是可产生磁性的杆体。
  10. 根据权利要求9所述的生化反应装置，其中所述反应杆是永久磁铁杆体或电磁铁杆体。
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