WO2019054834A2 - Sample measurement strip sensor - Google Patents

Sample measurement strip sensor Download PDF

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Publication number
WO2019054834A2
WO2019054834A2 PCT/KR2018/010938 KR2018010938W WO2019054834A2 WO 2019054834 A2 WO2019054834 A2 WO 2019054834A2 KR 2018010938 W KR2018010938 W KR 2018010938W WO 2019054834 A2 WO2019054834 A2 WO 2019054834A2
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sample
spacer
inlet
present
diaphragm member
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PCT/KR2018/010938
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French (fr)
Korean (ko)
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WO2019054834A3 (en
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허윤석
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계명대학교 산학협력단
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Publication of WO2019054834A3 publication Critical patent/WO2019054834A3/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Definitions

  • the present invention relates to a sample measurement strip sensor capable of analyzing a plurality of substances in a plurality of reaction chips by placing a reaction chip on the inner surface of a diaphragm member having a cross section in which regular polygons are divided by the same area, will be.
  • a biosensor is a system that converts biological signals into useful signals, such as color, fluorescence, and electrical signals, using biological elements or imitating biological elements when obtaining information from a measurement object.
  • biosensors are future fusion technologies that require technologies across the whole science including electronics, chemistry, biology, materials engineering, and enzyme engineering.
  • biosensors are medical, environmental, food, industrial, military, and experimental research. Of these, medical devices are the most widely used field, and biosensors for measuring blood glucose mainly occupy the majority of the market.
  • This biosensor has a small amount of required sample in terms of technology, high accuracy of measurement, less influence of surrounding environment, easy measurement, real time measurement is possible. In addition, it is a product in the early stage of entering the market from the market point of view.
  • examples of the sample introduction portion of the biosensor can be largely divided into vertical and horizontal structures.
  • the sample introduction portion of the vertical structure has a structure in which a hole is formed at the end of the sample introduction passage on the vertical line to form a capillary.
  • a vertically structured sample introduction part often has a disadvantage in that the amount of the injected sample may be changed due to the phenomenon that the end part of the passage is not completely filled or overflowed depending on the viscosity of the sample injected through the sample introduction path.
  • the sample introduction portion of the horizontal structure has a structure that forms a passage on the horizontal line from the side to the opposite side of the sensor.
  • Such a horizontal sample introduction part inconveniently introduces a sample from the side, and it is difficult to use it in a strip for a biosensor having multiple channels as in the case of a vertical sample introduction part.
  • biosensors and strips can be used only for analyzing one characteristic, and when one biosensor strip is provided with several channels, samples are mixed and it is difficult to measure each channel.
  • the present invention can simultaneously analyze a plurality of substances in a plurality of reaction chips constituting a single body, and can introduce a small amount of sample without a pretreatment process, thereby efficiently analyzing various samples and efficiently diagnosing various diseases
  • the strip sensor has a simple structure and is easy to manufacture.
  • the air discharge port which is perpendicular to the direction of sample injection, efficiently discharges the air to the outside during sample suction, thereby improving the sample suction speed. And the like.
  • the sample measurement strip sensor includes a diaphragm member having a cross section divided into equal polygons by a same area and a plurality of reaction chips disposed in the divided spaces of the diaphragm member and capable of simultaneously measuring a plurality of factors .
  • the diaphragm member according to the present invention is formed by extending the diaphragm outwardly around the center axis, and a plurality of spaces accommodating the reaction chip are radially divided.
  • the reaction chip according to the present invention includes a first spacer which is fixed by being interposed in the space and is formed with a first inlet for guiding the sample to be introduced into the front end, and a second spacer which is laminated on the first spacer, And an electrode substrate which is stacked on the second spacer and whose electrical characteristics are changed by a factor included in the sample at a position corresponding to the second inflow portion.
  • the first spacer according to the present invention is spaced apart from the first inlet and includes an air outlet for discharging the air to the outside when the sample flows into the first inlet.
  • an air discharge groove may be formed on a position corresponding to the air discharge port.
  • the sample measurement strip sensor according to an embodiment of the present invention has the following effects.
  • the structure of the strip sensor is simple and easy to manufacture, and the air outlet at a right angle to the sample injection direction effectively discharges the air to the outside during the sample intake, thereby improving the sample suction speed.
  • FIG. 1 is an exemplary view showing that two reaction chips are provided among sample sensor strip sensors according to an embodiment of the present invention.
  • FIG. 2 is an exemplary view showing that three reaction chips among the sample measurement strip sensors according to one embodiment of the present invention are provided.
  • FIG. 3 is an exemplary view showing that four and six reaction chips are provided among the sample measurement strip sensors according to one embodiment of the present invention.
  • the present invention relates to a diaphragm member having a cross section in which a regular polygon is divided into equal areas and a plurality of reaction chips disposed in the divided spaces of the diaphragm members and capable of simultaneously measuring a plurality of factors, It is possible to simultaneously analyze a plurality of substances in a plurality of reaction chips formed and to introduce a small amount of sample without a pretreatment process so that it can be efficiently applied to analysis of various samples and diagnosis of various diseases.
  • the present invention relates to a sample measurement strip sensor capable of analyzing a plurality of substances in a plurality of reaction chips by placing a reaction chip on the inner surface of a diaphragm member having a cross section in which regular polygons are divided by the same area, will be.
  • a strip sensor according to an embodiment of the present invention includes a diaphragm member 100 and a plurality of reaction chips 200 coupled to the diaphragm member 100.
  • the diaphragm member 100 is made of an insulating material that does not allow electricity.
  • the cross-sectional shape of the diaphragm member 100 is divided into regular polygons by the same area.
  • Each of the plurality of reaction chips 200 accommodated in the space includes a first spacer 210, a second spacer 220 and an electrode substrate 230 to form a reaction chip 200.
  • the first spacer 210 is fixed to the space of the diaphragm member 100, and a first inlet 211 for introducing the sample into the front end of the reaction chip 200, .
  • the first inlet 211 may be formed by cutting a part of the front end of the first spacer 210 into a square shape and may be formed at a distance from the first inlet 211 to the rear of the air outlet 212 .
  • the air outlet 212 induces the air in the space of the first inlet 211 to be discharged to the outside according to the flow of the sample, Smooth flow.
  • a second spacer 220 is stacked on the first spacer 210.
  • a second inlet 221 for introducing the sample into the front end of the second spacer 220 is formed,
  • the inlet 221 is also preferably formed by cutting a part of the front end of the second inlet 221.
  • the cutting length of the second inlet 221 is equal to the width of the first inlet 211 of the first spacer 210 while the cutting length of the second inlet 221 is equal to the width of the first inlet 210.
  • the strip sensor of the present invention has a simple structure, is easy to manufacture, and the air outlet, which is perpendicular to the sample injection direction, allows the air to be efficiently discharged to the outside during sample aspiration, .
  • the electrode substrate 230 is stacked on the second spacer 220.
  • the electrode substrate 230 is electrically connected to the second inlet 221 at a position corresponding to the second inlet 221 by a measurement factor included in the sample, , Voltage, current) changes.
  • the electrode substrate 230 includes a substrate 231, an electrode unit 232 and a terminal unit 233.
  • the substrate 231 is preferably made of a polymer resin material having electrical insulation properties.
  • the electrically insulating polymer resin include polyesters such as polyester, polycarbonate, polystyrene, polyimide, polyvinyl chloride, polyethylene, polyethyleneterephthalate ) Can be used.
  • the electrode 232 and the terminal 233 are formed on the surface of the substrate 231.
  • the electrode 232 is formed on the surface of the substrate 231 that is in contact with the second inlet 221.
  • the electrical characteristics such as electrical resistance, impedance, and the like change due to the influent sample, and are divided into a working electrode, a first reference electrode, and a second reference electrode.
  • the configuration of the electrode 232 can be variously changed depending on the manner of detecting the electrical characteristic.
  • the configuration described above is merely an example, and the configuration of the electrode is not limited to the above-described configuration.
  • the electrodes 232 are preferably formed by a method such as screen printing.
  • the electrode 232 provides the terminal 233 with an electrical characteristic changed by a factor included in the sample introduced into the electrode 232.
  • the terminal portion 233 is divided into a working electrode terminal electrically connected to each electrode, a first reference electrode terminal, and a second reference electrode terminal, and the electrical characteristics changed from the electrodes 232 are provided to an external lead
  • the terminal portion 233 may be exposed to the outside to be electrically connected to the reader.
  • the present invention can simultaneously analyze a plurality of materials in a plurality of reaction chips 200 constituting a single body, and can introduce a small amount of sample without a pretreatment process, and can analyze various samples and diagnose various diseases It is possible to efficiently apply the present invention.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention provides a sample measurement strip sensor comprising: a diaphragm member having a cross section in which a regular polygon is divided into equal areas; and a plurality of reaction chips that are disposed respectively in the divided spaces of the diaphragm member and may simultaneously measure a plurality of factors. The present invention enables simultaneous analysis of a plurality of materials in the plurality of reaction chips constituting a single body and introduction of a small amount of a sample without a pretreatment process, whereby the present invention can be efficiently applied to the analysis of various samples and diagnosis of various diseases, etc., the structure of the strip sensor is simple, it is easy to manufacture, and an air vent at a right angle to the direction of sample injection improves sample suction speed by very effectively venting the air to the outside during sample suction.

Description

시료측정 스트립 센서Sample measurement strip sensor
본 발명은 정다각형을 동일한 면적으로 분할한 단면을 가지는 격막부재의 내측면에 각각 반응칩을 배치하여, 한 번의 시료 주입으로 복수 개의 반응칩에서 복수 개의 물질을 분석할 수 있는 시료측정 스트립센서에 관한 것이다.The present invention relates to a sample measurement strip sensor capable of analyzing a plurality of substances in a plurality of reaction chips by placing a reaction chip on the inner surface of a diaphragm member having a cross section in which regular polygons are divided by the same area, will be.
일반적으로 바이오센서는 측정 대상물로부터 정보를 얻을 때, 생물학적 요소를 이용하거나 또는 생물학적 요소를 모방하여 색, 형광, 전기적 신호 등과 같이 인식 가능한 유용한 신호로 변환시켜주는 시스템이라고 할 수 있다.Generally, a biosensor is a system that converts biological signals into useful signals, such as color, fluorescence, and electrical signals, using biological elements or imitating biological elements when obtaining information from a measurement object.
따라서 바이오센서는 전자공학ㆍ화학ㆍ생물학ㆍ재료공학ㆍ효소공학 등 과학 전반에 걸친 기술을 필요로 하는 미래형 융합기술(fusion technology)이다.Therefore, biosensors are future fusion technologies that require technologies across the whole science including electronics, chemistry, biology, materials engineering, and enzyme engineering.
그리고 바이오센서의 주요 용도분야는 의료용, 환경용, 식품용, 산업용, 군사용, 실험연구용 등이다. 이 중 의료용은 현재 가장 많이 이용되고 있는 분야로서 주로 혈당측정용 바이오센서가 시장규모의 대부분을 차지하고 있다.The main applications of biosensors are medical, environmental, food, industrial, military, and experimental research. Of these, medical devices are the most widely used field, and biosensors for measuring blood glucose mainly occupy the majority of the market.
이러한 바이오센서는 기술적 측면에서 필요 시료량이 적고, 측정의 정확성이 높으며, 주변 환경의 영향이 적고, 측정이 간편하며, 측정의 리얼 타임화가 가능하다. 또한, 시장적 측면에서 시장진입 초기단계 제품이고, 용도분야가 다양하며, 높은 성장성이 전망되는 제품이다.This biosensor has a small amount of required sample in terms of technology, high accuracy of measurement, less influence of surrounding environment, easy measurement, real time measurement is possible. In addition, it is a product in the early stage of entering the market from the market point of view.
최근의 연구개발 추세는 최소량의 시료를 이용하는 바이오센서, 일회용 바이오센서를 개발하는 데 역점을 두고 있는 실정으로, 이러한 목적을 달성하기 위한 바이오센서 제조에 있어서의 기술적 측면에 대한 연구가 많이 수행되고 있다.Recently, research and development tendency has been focused on developing a biosensor and a disposable biosensor using a minimum amount of sample, and a lot of researches on the technical aspects in manufacturing a biosensor to accomplish this purpose have been carried out .
종래의 바이오센서의 구조적 측면을 살펴보면, 바이오센서의 시료 도입부의 예로는 크게 수직형 및 수평형 구조로 분류할 수 있다.As a structural aspect of a conventional biosensor, examples of the sample introduction portion of the biosensor can be largely divided into vertical and horizontal structures.
수직형 구조의 시료 도입부는 모세관을 형성하기 위하여 수직선상의 시료 도입 통로 끝 부분에 구멍이 뚫린 구조를 갖는다. 그러나 이러한 수직형 구조의 시료 도입부는 시료 도입 통로를 통하여 주입된 시료의 점도에 따라 종종 통로 끝부분을 완전히 채우지 못하거나 넘치는 현상이 발생하여 주입되는 시료의 양이 변화될 수 있는 단점이 있다. The sample introduction portion of the vertical structure has a structure in which a hole is formed at the end of the sample introduction passage on the vertical line to form a capillary. However, such a vertically structured sample introduction part often has a disadvantage in that the amount of the injected sample may be changed due to the phenomenon that the end part of the passage is not completely filled or overflowed depending on the viscosity of the sample injected through the sample introduction path.
반면, 수평형 구조의 시료 도입부는 센서의 측면에서 반대 측면까지 수평선상의 통로를 형성하는 구조를 갖는다.On the other hand, the sample introduction portion of the horizontal structure has a structure that forms a passage on the horizontal line from the side to the opposite side of the sensor.
이러한 수평형 시료 도입부는 측면에서 시료의 도입이 이루어져야 하는 불편함이 있고, 수직형 시료 도입부와 마찬가지로 여러 채널을 가지는 바이오센서용 스트립에 사용하기에는 어려움이 있다.Such a horizontal sample introduction part inconveniently introduces a sample from the side, and it is difficult to use it in a strip for a biosensor having multiple channels as in the case of a vertical sample introduction part.
따라서 종래 바이오센서용 스트립의 경우, 반응부로부터 스트립 외부로 직접 연결되는 별도의 공기배출터널이 형성되어 있지 않아 시료가 반응부에 유입된 후 확산되는 속도가 늦어 분석 시간이 많이 걸리는 단점이 있다.Therefore, in the conventional biosensor strip, there is no separate air discharge tunnel directly connected to the outside of the strip from the reaction part, and the analysis time is long because the sample is diffused after being introduced into the reaction part.
또한, 종래의 바이오센서 및 스트립은 단순히 한 가지 특징을 분석할 때만 사용할 수 있고 한 개의 바이오센서용 스트립으로 여러 채널을 구비할 때는 시료가 섞이게 되어 각각의 채널을 측정하는 데 어려움이 있다.In addition, conventional biosensors and strips can be used only for analyzing one characteristic, and when one biosensor strip is provided with several channels, samples are mixed and it is difficult to measure each channel.
본 발명은 하나의 몸체를 이루는 복수 개의 반응칩에서 복수 개의 물질을 동시에 분석할 수 있고, 적은 양의 시료를 전처리 과정 없이 도입할 수 있어, 다양한 시료의 분석 및 각종 질병 등의 진단에 효율적으로 적용할 수 있으며, 스트립 센서의 구조가 단순하고, 제조가 용이하며, 시료주입방향과 직각을 이루는 공기배출구에 의해, 시료 흡입시 공기가 외부로 매우 효과적으로 배출됨으로써 시료흡입속도를 향상시키는 시료측정 스트립 센서를 제공하는 것을 그 목적으로 한다.The present invention can simultaneously analyze a plurality of substances in a plurality of reaction chips constituting a single body, and can introduce a small amount of sample without a pretreatment process, thereby efficiently analyzing various samples and efficiently diagnosing various diseases The strip sensor has a simple structure and is easy to manufacture. The air discharge port, which is perpendicular to the direction of sample injection, efficiently discharges the air to the outside during sample suction, thereby improving the sample suction speed. And the like.
본 발명에 따른 시료측정 스트립 센서는 정다각형을 동일한 면적으로 분할한 단면을 가지는 격막부재 및 상기 격막부재의 분할된 공간에 각각 배치되어, 복수 개의 인자를 동시에 측정할 수 있는 복수 개의 반응칩을 포함한다.The sample measurement strip sensor according to the present invention includes a diaphragm member having a cross section divided into equal polygons by a same area and a plurality of reaction chips disposed in the divided spaces of the diaphragm member and capable of simultaneously measuring a plurality of factors .
이때 본 발명에 따른 상기 격막부재는 중심을 축으로 격막을 외향으로 연장형성하여, 반응칩이 수용되는 복수 개의 공간이 방사상으로 분할 형성된다.At this time, the diaphragm member according to the present invention is formed by extending the diaphragm outwardly around the center axis, and a plurality of spaces accommodating the reaction chip are radially divided.
그리고 본 발명에 따른 상기 반응칩은 상기 공간에 면접하여 고정되고, 전단에는 시료가 유입되도록 유도하는 제1유입부를 형성한 제1스페이서와, 상기 제1스페이서 상에 적층되고, 전단에는 시료가 유입되도록 유도하는 제2유입부를 형성한 제2스페이서와, 상기 제2스페이서 상에 적층되고, 상기 제2유입부와 대응하는 위치에 시료에 포함된 인자에 의해 전기적 특성이 변화되는 전극기판으로 이루어진다.In addition, the reaction chip according to the present invention includes a first spacer which is fixed by being interposed in the space and is formed with a first inlet for guiding the sample to be introduced into the front end, and a second spacer which is laminated on the first spacer, And an electrode substrate which is stacked on the second spacer and whose electrical characteristics are changed by a factor included in the sample at a position corresponding to the second inflow portion.
여기서 본 발명에 따른 상기 제1스페이서는 제1유입부와 거리를 두고 이격 형성되어, 상기 제1유입부에 시료가 유입될 시, 공기가 외부로 배출되도록 하는 공기배출구를 포함한다.The first spacer according to the present invention is spaced apart from the first inlet and includes an air outlet for discharging the air to the outside when the sample flows into the first inlet.
또한, 본 발명에 따른 상기 격막부재에는 상기 공기배출구와 대응하는 위치상에 공기배출홈을 형성할 수 있다.Further, in the diaphragm member according to the present invention, an air discharge groove may be formed on a position corresponding to the air discharge port.
본 발명의 일 실시예에 따른 시료측정 스트립 센서는 다음과 같은 효과를 가진다.The sample measurement strip sensor according to an embodiment of the present invention has the following effects.
첫째, 하나의 몸체를 이루는 복수 개의 반응칩에서 복수 개의 물질을 동시에 분석할 수 있고, 적은 양의 시료를 전처리 과정 없이 도입할 수 있어, 다양한 시료의 분석 및 각종 질병 등의 진단에 효율적으로 적용할 수 있는 효과를 가진다.Firstly, it is possible to simultaneously analyze a plurality of substances in a plurality of reaction chips constituting a single body, and to introduce a small amount of sample without a pretreatment process, and to efficiently analyze various samples and diagnose various diseases It has the effect of being able to.
둘째, 스트립 센서의 구조가 단순하고, 제조가 용이하며, 시료주입방향과 직각을 이루는 공기배출구에 의해, 시료 흡입시 공기가 외부로 매우 효과적으로 배출됨으로써 시료흡입속도를 향상시키는 효과를 가진다.Secondly, the structure of the strip sensor is simple and easy to manufacture, and the air outlet at a right angle to the sample injection direction effectively discharges the air to the outside during the sample intake, thereby improving the sample suction speed.
도 1은 본 발명의 일 실시에 따른 시료측정 스트립 센서 중 2개의 반응칩이 구비된 것을 보인 예시도이다.FIG. 1 is an exemplary view showing that two reaction chips are provided among sample sensor strip sensors according to an embodiment of the present invention.
도 2는 본 발명의 일 실시에 따른 시료측정 스트립 센서 중 3개의 반응칩이 구비된 것을 보인 예시도이다.FIG. 2 is an exemplary view showing that three reaction chips among the sample measurement strip sensors according to one embodiment of the present invention are provided.
도 3은 본 발명의 일 실시에 따른 시료측정 스트립 센서 중 4개 및 6개의 반응칩이 구비된 것을 보인 예시도이다.FIG. 3 is an exemplary view showing that four and six reaction chips are provided among the sample measurement strip sensors according to one embodiment of the present invention.
본 발명은 정다각형을 동일한 면적으로 분할한 단면을 가지는 격막부재와, 상기 격막부재의 분할된 공간에 각각 배치되어, 복수 개의 인자를 동시에 측정할 수 있는 복수 개의 반응칩을 포함하여, 하나의 몸체를 이루는 복수 개의 반응칩에서 복수 개의 물질을 동시에 분석할 수 있고, 적은 양의 시료를 전처리 과정 없이 도입할 수 있어, 다양한 시료의 분석 및 각종 질병 등의 진단에 효율적으로 적용할 수 있다.The present invention relates to a diaphragm member having a cross section in which a regular polygon is divided into equal areas and a plurality of reaction chips disposed in the divided spaces of the diaphragm members and capable of simultaneously measuring a plurality of factors, It is possible to simultaneously analyze a plurality of substances in a plurality of reaction chips formed and to introduce a small amount of sample without a pretreatment process so that it can be efficiently applied to analysis of various samples and diagnosis of various diseases.
이하, 첨부된 도면을 참조하여 본 발명에 따른 바람직한 실시 예를 상세히 설명하기로 한다. 이에 앞서, 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여, 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately The present invention should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention.
따라서 본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 가장 바람직한 실시 예에 불과할 뿐이고, 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들은 대체할 수 있는 균등한 변형 예들이 있을 수 있음을 이해하여야 한다.Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention, and not all of the technical ideas of the present invention are described. Therefore, at the time of the present application, It should be understood that variations can be made.
본 발명은 정다각형을 동일한 면적으로 분할한 단면을 가지는 격막부재의 내측면에 각각 반응칩을 배치하여, 한 번의 시료 주입으로 복수 개의 반응칩에서 복수 개의 물질을 분석할 수 있는 시료측정 스트립센서에 관한 것이다.The present invention relates to a sample measurement strip sensor capable of analyzing a plurality of substances in a plurality of reaction chips by placing a reaction chip on the inner surface of a diaphragm member having a cross section in which regular polygons are divided by the same area, will be.
도 1 내지 도 3을 참조한 본 발명의 일 실시예에 따른 스트립센서는 격막부재(100)와, 상기 격막부재(100)에 결합하는 복수 개의 반응칩(200)을 포함한다.1 to 3, a strip sensor according to an embodiment of the present invention includes a diaphragm member 100 and a plurality of reaction chips 200 coupled to the diaphragm member 100.
이때 상기 격막부재(100)는 전기가 통하지 않는 절연재로 이루어지는 것이 바람직하고, 복수 개의 반응칩(200)을 결합하기 위해 그 단면 형상은 정다각형을 동일한 면적으로 분할된 형태를 이룬다.At this time, it is preferable that the diaphragm member 100 is made of an insulating material that does not allow electricity. In order to couple a plurality of reaction chips 200, the cross-sectional shape of the diaphragm member 100 is divided into regular polygons by the same area.
즉, 도 2 및 도 3에 도시한 바와 같이 상기 격막부재(100)의 길이방향 중심을 축으로 복수 개의 공간이 방사상으로 분할되는데, 이때 반응칩(200)들 사이에 절연을 위해 상기 격막부재(100)의 중심에서 외향으로 격막(101)이 연장 형성되어, 상기 격막(101)들 사이에 상기 반응칩(200) 수용되는 공간이 방사상으로 분할된다.That is, as shown in FIGS. 2 and 3, a plurality of spaces are radially divided along the longitudinal center of the diaphragm member 100, and the diaphragm member 100 The space in which the reaction chip 200 is accommodated is radially divided between the septa 101 and the partition 101. [
그리고 상기 공간에 수용되는 복수 개의 반응칩(200) 각각은 제1스페이서(210), 제2스페이서(220) 및 전극기판(230)이 적층되어 하나의 몸체로 이루어져 반응칩(200)을 이룬다.Each of the plurality of reaction chips 200 accommodated in the space includes a first spacer 210, a second spacer 220 and an electrode substrate 230 to form a reaction chip 200.
상기 반응칩(200)을 보다 상세하게 살펴보면, 먼저 상기 제1스페이서(210)는 상기 격막부재(100)의 공간에 면접하여 고정되고, 전단에는 시료가 유입되도록 유도하는 제1유입부(211)를 형성한다.First, the first spacer 210 is fixed to the space of the diaphragm member 100, and a first inlet 211 for introducing the sample into the front end of the reaction chip 200, .
이때 상기 제1유입부(211)는 제1스페이서(210)의 전단 중 일부를 사각형태로 절단하여 형성하는 것이 바람직하고, 상기 제1유입부(211)와 거리를 두고 배후에 공기배출구(212)를 이격 형성한다.The first inlet 211 may be formed by cutting a part of the front end of the first spacer 210 into a square shape and may be formed at a distance from the first inlet 211 to the rear of the air outlet 212 .
상기 공기배출구(212)는 상기 제1유입부(211)로 시료가 유입될 시, 상기 제1유입부(211) 공간에 있던 공기가 시료 유입에 따라 외부로 배출되도록 공기를 유도하여, 시료의 유입을 원활하게 한다.When the sample flows into the first inlet 211, the air outlet 212 induces the air in the space of the first inlet 211 to be discharged to the outside according to the flow of the sample, Smooth flow.
또한, 상기 제1스페이서(210) 상에는 제2스페이서(220)가 적층되는데, 상기 제2스페이서(220)의 전단에는 시료가 유입되도록 유도하는 제2유입부(221)를 형성하는데, 상기 제2유입부(221) 역시, 상기 제2유입부(221)의 전단 중 일부를 절단하여 형성하는 것이 바람직하다.A second spacer 220 is stacked on the first spacer 210. A second inlet 221 for introducing the sample into the front end of the second spacer 220 is formed, The inlet 221 is also preferably formed by cutting a part of the front end of the second inlet 221.
이때 상기 제2유입부(221)의 절단 폭은 상기 제1스페이서(210)의 상기 제1유입부(211)의 폭과 동일하나, 상기 제2유입부(221)의 절단 길이는 상기 제1스페이서(210)의 공기배출구(212)가 형성된 위치까지 절단되어, 상기 제1스페이서(210) 상에 제2스페이서(220)가 적층되어도 상기 제1유입부(211)로 시료가 유입될 시, 상기 제1유입구(211)의 공기가 상기 제2유입부(221)를 통해 공기배출구(212)로 안내되도록 한다.The cutting length of the second inlet 221 is equal to the width of the first inlet 211 of the first spacer 210 while the cutting length of the second inlet 221 is equal to the width of the first inlet 210. [ When the sample is introduced into the first inlet 211 even though the second spacer 220 is stacked on the first spacer 210 after the air outlet 212 of the spacer 210 is formed, So that the air in the first inlet 211 is guided to the air outlet 212 through the second inlet 221.
상기한 구성에 의해 본 발명에 따른 스트립 센서는 그 구조가 단순하고, 제조가 용이하며, 시료주입방향과 직각을 이루는 공기배출구에 의해, 시료 흡입시 공기가 외부로 매우 효과적으로 배출됨으로써 시료흡입속도를 향상시킨다.According to the above configuration, the strip sensor of the present invention has a simple structure, is easy to manufacture, and the air outlet, which is perpendicular to the sample injection direction, allows the air to be efficiently discharged to the outside during sample aspiration, .
그리고 상기 제2스페이서(220) 상에는 전극기판(230)이 적층되는데, 상기 전극기판(230)은 상기 제2유입부(221)와 대응하는 위치에 시료에 포함된 측정 인자에 의해 전기적 특성(저항, 전압, 전류)이 변화한다.The electrode substrate 230 is stacked on the second spacer 220. The electrode substrate 230 is electrically connected to the second inlet 221 at a position corresponding to the second inlet 221 by a measurement factor included in the sample, , Voltage, current) changes.
상기 전극기판(230)은 기판(231), 전극부(232) 및 단자부(233)가 포함되는데, 상기 기판(231)은 전기 절연성을 갖는 고분자 수지 계열의 재질로 이루어지는 것이 바람직하다. 상기 전기 절연성을 갖는 고분자 수지로는 폴리에스테르(polyester), 폴리카보네이트(polycarbonate), 폴리스티렌(polystyrene), 폴리이미드(polyimide), 폴리비닐클로라이드(polyvinyl chloride), 폴리에틸렌(polyethylene), 폴리에틸렌테레프탈레이트(polyethyleneterephthalate) 등의 고분자 화합물을 사용할 수 있다.The electrode substrate 230 includes a substrate 231, an electrode unit 232 and a terminal unit 233. The substrate 231 is preferably made of a polymer resin material having electrical insulation properties. Examples of the electrically insulating polymer resin include polyesters such as polyester, polycarbonate, polystyrene, polyimide, polyvinyl chloride, polyethylene, polyethyleneterephthalate ) Can be used.
이때 상기 기판(231)의 표면에는 전극(232) 및 단자부(233)가 구비되는데, 상기 전극(232)은 상기 기판(231)의 제2유입부(221)와 면접하는 표면에 형성되어 외부로부터 유입된 시료에 의해 전기적 특성인 전기저항, 임피던스 등이 변화하는 것으로서, 작업 전극, 제1기준전극 및 제2기준전극으로 구분된다.The electrode 232 and the terminal 233 are formed on the surface of the substrate 231. The electrode 232 is formed on the surface of the substrate 231 that is in contact with the second inlet 221, The electrical characteristics such as electrical resistance, impedance, and the like change due to the influent sample, and are divided into a working electrode, a first reference electrode, and a second reference electrode.
하지만, 전극(232)의 구성은 전기적 특성을 검출하는 방식에 따라 다양하게 변경 가능한 것으로서, 전술한 구성은 예시적으로 설명한 것에 불과하며 전극의 구성을 전술한 형태에 한정하는 것은 아니다. However, the configuration of the electrode 232 can be variously changed depending on the manner of detecting the electrical characteristic. The configuration described above is merely an example, and the configuration of the electrode is not limited to the above-described configuration.
또한, 상기 전극(232)들은 스크린 프린트 등의 방법으로 형성되는 것이 바람직하다.In addition, the electrodes 232 are preferably formed by a method such as screen printing.
상기 전극(232)은 내부로 유입된 시료에 포함된 인자에 의해 변화된 전기적 특성을 상기 단자부(233)로 제공하게 된다.The electrode 232 provides the terminal 233 with an electrical characteristic changed by a factor included in the sample introduced into the electrode 232.
상기 단자부(233)는 각 전극들과 전기적으로 연결되는 작업전극단자, 제1기준전극단자 및 제2기준전극단자로 구분되어, 상기 각 전극(232)들로부터 변화된 전기적 특성을 외부의 리드기로 제공하도록, 상기 단자부(233)는 외부에 노출되어 리더기와 전기적으로 연결될 수 있게 된다.The terminal portion 233 is divided into a working electrode terminal electrically connected to each electrode, a first reference electrode terminal, and a second reference electrode terminal, and the electrical characteristics changed from the electrodes 232 are provided to an external lead The terminal portion 233 may be exposed to the outside to be electrically connected to the reader.
따라서 본 발명은 하나의 몸체를 이루는 복수 개의 반응칩(200)에서 복수 개의 물질을 동시에 분석할 수 있고, 적은 양의 시료를 전처리 과정 없이 도입할 수 있어, 다양한 시료의 분석 및 각종 질병 등의 진단에 효율적으로 적용할 수 있다.Therefore, the present invention can simultaneously analyze a plurality of materials in a plurality of reaction chips 200 constituting a single body, and can introduce a small amount of sample without a pretreatment process, and can analyze various samples and diagnose various diseases It is possible to efficiently apply the present invention.
본 발명은 도면에 도시된 실시 예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 본 기술 분야의 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 다른 실시 예가 가능하다는 점을 이해할 것이다. 따라서 본 발명의 진정한 기술적 보호 범위는 첨부된 특허청구범위의 기술적 사상에 의하여 정해져야 할 것이다.While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (5)

  1. 정다각형을 동일한 면적으로 분할한 단면을 가지는 격막부재;A diaphragm member having a cross section obtained by dividing regular polygons into equal areas;
    상기 격막부재의 분할된 공간에 각각 배치되어, 복수 개의 인자를 동시에 측정할 수 있는 복수 개의 반응칩을 포함하는 시료측정 스트립 센서.And a plurality of reaction chips disposed in the divided spaces of the diaphragm members and capable of simultaneously measuring a plurality of factors.
  2. 청구항 1에 있어서,The method according to claim 1,
    상기 격막부재는 The diaphragm member
    중심을 축으로 격막을 외향으로 연장형성하여, 반응칩이 수용되는 복수 개의 공간이 방사상으로 분할 형성되는 시료측정 스트립 센서.Wherein a plurality of spaces in which the reaction chip is accommodated are radially divided and formed by extending the diaphragm outwardly around the center axis.
  3. 청구항 1에 있어서,The method according to claim 1,
    상기 반응칩은The reaction chip
    상기 공간에 면접하여 고정되고, 전단에는 시료가 유입되도록 유도하는 제1유입부를 형성한 제1스페이서와;A first spacer fixed to the space by being fixed thereto and having a first inlet portion for introducing the sample into the front end;
    상기 제1스페이서 상에 적층되고, 전단에는 시료가 유입되도록 유도하는 제2유입부를 형성한 제2스페이서와;A second spacer stacked on the first spacer, the second spacer having a second inlet portion for introducing the sample into the front end;
    상기 제2스페이서 상에 적층되고, 상기 제2유입부와 대응하는 위치에 시료에 포함된 인자에 의해 전기적 특성이 변화되는 전극기판으로 이루어진 시료측정 스트립 센서.And an electrode substrate laminated on the second spacer and having electrical characteristics changed by a factor included in the sample at a position corresponding to the second inlet.
  4. 청구항 3에 있어서,The method of claim 3,
    상기 제1스페이서는 The first spacer
    제1유입부와 거리를 두고 이격 형성되어, 상기 제1유입부에 시료가 유입될 시, 공기가 외부로 배출되도록 하는 공기배출구를 포함하는 스트립 센서.And an air outlet formed at a distance from the first inlet to allow the air to be discharged to the outside when the sample flows into the first inlet.
  5. 청구항 4에 있어서,The method of claim 4,
    상기 격막부재에는 The diaphragm member
    상기 공기배출구와 대응하는 위치상에 공기배출홈을 형성한 스트립 센서.And an air discharge groove is formed on a position corresponding to the air discharge port.
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KR101363020B1 (en) * 2011-10-31 2014-02-26 주식회사 세라젬메디시스 A biosensor for multiple reaction
KR20130115675A (en) * 2012-04-13 2013-10-22 주식회사 티포아이텍 Biosensor
KR20150111100A (en) * 2014-03-25 2015-10-05 희성금속 주식회사 Electrochemical biosensor strip
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