CN103424451B - A kind of card form potassium ion sensor and preparation method thereof - Google Patents
A kind of card form potassium ion sensor and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to electrochemical sensor technology field, particularly relate to a kind of card form potassium ion sensor and preparation method thereof.Sensor of the present invention comprises base plate (1), parallel two potassium ion-selective electrodes (2) on base plate (1), insulation course (3), salt bridge (4), described potassium ion-selective electrode (2) comprises electrode basement system (20), dielectric substrate (21), potassium ion sensitive membrane (22), electrode basement system (20) is by the reaction electrode (201) be positioned on base plate (1), contact electrode (202) and connect these two electrodes conductive lead wire (203) composition, described dielectric substrate (21) is positioned on the reaction electrode (201) of electrode basement system, dielectric substrate (21) surrounds by described potassium ion sensitive membrane (22).Sensor of the present invention is easy to carry, have simple to operate, response rapidly, the advantage of working stability.
Description
Technical field
The present invention relates to electrochemical sensor technology field, particularly relate to a kind of card form potassium ion sensor and preparation method thereof.
Background technology
Potassium is indispensable macroelement in human body, and general adult human body includes potassium element about 150 grams.Its effect of potassium in human body maintains normal function that is neural, muscle, is the normal osmotic pressure and the acid base equilibrium that keep body, involved in sugar and protein metabolism, ensures that nervimuscular normal function institute is required.Human body potassium deficiency, can cause acid-base imbalance in body, metabolic disorder, muscle weakness etc.Therefore.The detection of potassium ion has a wide range of applications in all many-sides such as medical science, biochemistry, pharmacy, chemical industry, environmental protection and food, and particularly in human body fluid, the detection of potassium ion has important clinical medicine meaning.
The detection of current potassium concentration mainly adopts the blomelicalbloodgasandelectrolrteanalyzers based on electrochemical method.Blomelicalbloodgasandelectrolrteanalyzers all adopts potassium ion-selective electrode and outer contrast electrode to form potassium ion sensor, these two electrodes are inserted in solution to be measured simultaneously, and connect and compose an electrochemical cell with external measurement mechanism, measure the electromotive force of this electrochemical cell, potassium concentration in solution to be measured can be extrapolated according to Nernst equation.Traditional potassium ion-selective electrode is made up of internal reference electrode, interior reference solution and sensitive membrane, with high costs, and volume is comparatively large, is difficult to the microminiaturization carrying out electrode, is not easy to carry.This kind of potassium ion-selective electrode must match with outer contrast electrode when actual measurement, testing process is numerous and diverse, low-response, and detection time is longer, the demand of the occasions such as operation, emergency treatment, field rescue cannot be met, also cannot adapt to the trend of modern medical service POCT pattern.。
Summary of the invention
The present invention be during the existing traditional potassium concentration of solution detects the electrodes selective of potassium ion sensor be separated with outer contrast electrode caused not portable, detect the problems such as consuming time, provide a kind of new-type card form potassium ion sensor.
A kind of card form potassium ion sensor of the present invention, its scheme is: comprise base plate, potassium ion-selective electrode, insulation course, salt bridge, two potassium ion-selective electrodes are set in parallel on base plate, described potassium ion-selective electrode comprises electrode basement system, dielectric substrate, potassium ion sensitive membrane, electrode basement system is made up of the reaction electrode be positioned on base plate, contact electrode and the conductive lead wire that connects these two electrodes, described dielectric substrate is positioned on the reaction electrode of electrode basement system, and dielectric substrate surrounds by described potassium ion sensitive membrane; Described potassium ion sensitive membrane scribbles insulation course, insulation course is provided with the exposed opening of potassium ion sensitive membrane, the opening of insulation course and potassium ion sensitive membrane form the reaction chamber of potassium ion-selective electrode, salt bridge is arranged on the surface of insulation course, and be positioned at the both sides of insulation course upper shed, salt bridge connects two potassium ion-selective electrodes, and salt bridge is covered with cover plate.
Further, on described cover plate, the position corresponding with salt bridge is provided with pore.
The present invention also provides the preparation method of above-mentioned card form potassium ion sensor, comprises the following steps:
(1) on high-molecular organic material base plate, coated with conductive material forms reaction electrode, the contact electrode and conductive lead wire of potassium ion-selective electrode;
According to actual needs, can adopt method for printing screen on high-molecular organic material (as polypropylene, polyester, tygon and Polyvinylchloride etc.) base plate, print conduction screen print materials, also exploitation by film evaporation technology by this conduction screen print materials coating by vaporization on high-molecular organic material base plate, form the reaction electrode of potassium ion-selective electrode, contact electrode and conductive lead wire;
Reaction electrode can adopt the materials such as carbon, gold, platinum or titanium to make, conductive lead wire with contact electrode the materials such as silver, carbon, gold, platinum or titanium can be adopted to make, the electrochemical properties of silver is unstable, but electric conductivity is good, therefore be not generally used for making reaction electrode, and can be used to make contact electrode and conductive lead wire;
(2) oxidizing solution is used to carry out surface treatment to the reaction electrode of potassium ion-selective electrode;
(3) inorganic salts containing potassium ion are dissolved in purified water, be mixed with the solution that concentration is 0.5mM ~ 5.0mM, then in this solution, the xanthans that final mass number percent is 3.0% ~ 8.0% is added, be mixed with electrolyte slurry, this electrolyte slurry is coated in reaction electrode surface (can adopt the method for serigraphy that this electrolyte slurry is coated in reaction electrode surface), forms dielectric substrate;
(4) potassium ion active carrier, the large molecule of lipophilicity, non-conductive polymer polymkeric substance, plasticizer dissolves are mixed with viscous solution in cyclohexanone or acetone, as potassium ion-selective electrode film liquid, in the gross mass of solution, wherein the mass percent of potassium ion active carrier is 0.2% ~ 0.5%, the macromolecular mass percent of lipophilicity is 0.5% ~ 1.0%, the mass percent of non-conductive polymer polymkeric substance is 30.0% ~ 35.0%, the mass percent of plastifier is 8.0% ~ 14.0%, remaining as solvent; Electrode film liquid is coated in the electrolyte layer surface of potassium ion-selective electrode, forms potassium ion sensitive membrane, lucifuge freeze-drying lyophilization is preserved;
(5) on base plate, print insulation course, insulation course covers potassium ion sensitive membrane, and insulation course is provided with the opening making potassium ion sensitive membrane exposed, the conversion zone that when this open area is actual measurement, potassium ion sensitive membrane and solution to be measured come in contact;
(6) two double faced adhesive tape colloids are affixed on respectively the both sides of insulation course upper shed, the length of colloid enough connects two potassium ion-selective electrodes, forms groove between two colloids;
(7) cover plate of surface hydrophilicity is covered in colloid surface, i.e. obtained card form potassium ion sensor.
Preferably,
The oxidizing solution that oxidation processes carried out to reaction electrode surface to be mass percent be 15% ~ 25% potassium bichromate solution or liquor potassic permanganate;
The described inorganic salts containing potassium ion are selected from one or more in sal tartari, dipotassium hydrogen phosphate, potassium sulfate;
Described potassium ion active carrier is selected from a word used in person's names amino doxycycline, the 4-tert-butyl group-2,2,14,14-tetra-with-2a, 14a, dioxy cup [4] aryl four acetic acid tetra-tert ester, two (phendioxin 5-crown ether-4)-4
,-ylmethyl pimelate, 2-dodecyl-2-methyl isophthalic acid, 3-glyceryl-bis--[N-(5
,-nitro (benzene-15-crown ether-5)-4
,base] one or more in carbamate;
The large molecule of described lipophilicity is selected from one or more in tetraphenylboron sodium, tetrachlorobenzene boron potassium, four [3,5-bis-(trifluoromethy) phenyl] potassium borate;
Described non-conductive polymer polymkeric substance is selected from one or more in Polyvinylchloride, polyurethane, polyvinyl acetate, polymethylmethacrylate;
Described plastifier is selected from one or more in ortho-nitrophenyl octyl ether, amyl phthalate, dioctyl phthalate, di-n-octyl sebacate, dibutyl sebacate;
Described salt bridge is selected from one or more in following material: filter paper, cotton, chemical fibre, nonwoven fabrics, agar, gel.
Traditional potassium ion-selective electrode and outer contrast electrode are integrated on a base plate by a kind of card form of the present invention potassium ion sensor, achieve the integrated and microminiaturized of potassium ion sensor, be easy to carry, have simple to operate, response rapidly, the advantage of working stability.The preparation method of card form potassium ion sensor of the present invention, simplifies Making programme, reduces cost of manufacture, is applicable to producing in enormous quantities.
Card form potassium ion sensor of the present invention, when actual measurement, by two potassium ion-selective electrodes in this card form potassium ion sensor respectively with the solution to be measured of unknown concentration and the reference solution of concentration known, two contact electrodes with periphery testing circuit be connected.Because the present invention is very microminiaturized, overall electrode area only has 1.5cm*2.3cm, open area on insulation course is less, between the colloid of insulation course upper shed both sides, spacing is very little, the syphonic effect of capillary of being tired out to death can be played, solution to be measured and reference solution are sucked respectively the reaction chamber of two potassium ion-selective electrodes.Therefore detect required test solution amount little, detect required time shorter.When detecting potassium concentration in serum, required blood sample only 10 ~ 15uL, detection time is less than 40s.
By the solution to be measured in difference sucting reaction chamber and reference solution, come in contact by the opening on insulation course and potassium ion sensitive membrane, and react with potassium ion sensitive membrane.In potassium ion sensitive membrane, potassium ion active carrier is generally caged, ring-type or chain organic compound, multiple polarity dentate containing oxygen atom is had in molecule, because oxygen atom has two pairs of lone pair electrons to provide dipole moment-ions binding power, can with the potassium ion complexing in solution to be measured, reference solution, form the complex compound of 1:1, thus change the membrane electrode current potential of potassium ion sensitive membrane.
Add the inorganic salts containing potassium ion in dielectric substrate, make to form Ohmic contact between dielectric substrate and reaction electrode, effectively reduce the resistance of electrode.
Accompanying drawing explanation
Fig. 1 is the vertical view of card form potassium ion sensor of the present invention;
Fig. 2 is the structural representation of potassium ion-selective electrode in card form potassium ion sensor of the present invention;
Fig. 3 is the potential response calibration curve of example 1 of the present invention in the KCl solution of variable concentrations;
Fig. 4 is the potential response calibration curve of potassium concentration in example of the present invention 1 pair of serum;
Fig. 5 is the response gradient relation over time of example 1 of the present invention;
Fig. 6 is the potential response calibration curve of example 2 of the present invention in the KCl solution of variable concentrations;
Fig. 7 is the potential response calibration curve of example 2 of the present invention in the serum containing variable concentrations potassium ion.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated, and protection scope of the present invention is not limited in these embodiments.
embodiment 1
A kind of card form potassium ion sensor, as Fig. 1, shown in Fig. 2, card form potassium ion sensor of the present invention, comprise base plate 1, potassium ion-selective electrode 2, insulation course 3, salt bridge 4, two potassium ion-selective electrodes are set in parallel on base plate 1, described potassium ion-selective electrode 2 comprises electrode basement system 20, dielectric substrate 21, potassium ion sensitive membrane 22, electrode basement system 20 is by the reaction electrode 201 be positioned on base plate 1, contact electrode 202 and the conductive lead wire 203 connecting these two electrodes form, described dielectric substrate 21 is positioned on the reaction electrode 201 of electrode basement system, dielectric substrate 21 surrounds by described potassium ion sensitive membrane 22, described potassium ion sensitive membrane 22 scribbles insulation course 3, insulation course 3 is provided with the exposed opening of potassium ion sensitive membrane, the opening of insulation course 3 and potassium ion sensitive membrane 22 form the reaction chamber 5 of potassium ion-selective electrode 2, salt bridge 4 is arranged on the surface of insulation course 3, and be positioned at the both sides of insulation course 3 upper shed, salt bridge 4 connects two potassium ion-selective electrodes 2, salt bridge 4 is covered with cover plate 6.As improvement project, position corresponding with salt bridge on cover plate is provided with pore.
Card form potassium ion sensor preparation method of the present invention, step is as follows:
(1) silver-colored printing material is printed on polypropylene base plate by screen printing technique, forms the reaction electrode of potassium ion-selective electrode; Platinum printing material is printed on polypropylene base plate by screen printing technique, forms the conductive lead wire connected with reaction electrode and contact electrode;
(2) absolute ethyl alcohol is used to clean reaction electrode surface, then wiped clean;
(3) reaction electrode being dipped into mass percent is 10min in the liquor potassic permanganate of 20%, and effects on surface carries out oxidation processes, processes rear purified water and rinses well, dries in 80 DEG C of baking ovens;
(4) by sal tartari (K
2cO
3, Shanghai traditional Chinese medicines group) be dissolved in purified water and form the solution that concentration is 4.5mM concentration, until completely dissolved, add the xanthans (Shanghai traditional Chinese medicines group) that mass percent is 5%, fully stir evenly, form the dielectric substrate slurry of clear viscous, by the method for serigraphy, this dielectric substrate slurry is printed on the reaction electrode surface of potassium ion-selective electrode, form dielectric substrate, thickness is about 50um, and web plate used is 250 orders, and net is apart from 1.5mm, scraper velocity 15mm/s, pressure 2.5kg/cm
2;
(5) in 2.2ml dibutyl sebacate, 18.9mg a word used in person's names amino doxycycline, the tetraphenylboron sodium of 17.0mg and the PVC of 1120mg is added, shake after 1 minute, add 8ml acetone, concussion is dissolved and is obtained the potassium ion film forming liquid of homogeneous transparent thickness, by the method for serigraphy by the electrolyte layer surface of this potassium ion film forming liquid at potassium ion-selective electrode, form potassium ion sensitive membrane, thickness is about 10um, and web plate used is 150 orders, and net is apart from 1.5mm, scraper velocity 15mm/s, pressure 2.5kg/cm
2;
(6) method of serigraphy is adopted to print insulation course, insulation course surrounds potassium ion sensitive membrane, insulation course is provided with the circular open of two diameter 3mm, make potassium ion sensitive membrane exposed, as the conversion zone of potassium ion sensitive membrane, potassium ion sensitive membrane is contacted with solution to be measured, reference solution in measuring process by opening, and insulating layer material adopts the insulation dyestuff SS8391 that in precious magnificent cause, Co., Ltd produces;
(7) double-sided tape (3M) of two wide 0.5cm, thick 0.25mm is affixed on the both sides of insulation course upper shed, between two adhesive tape, forms the wide groove of 2mm;
(8) surface hydrophilicity cover plate is covered in colloid surface, forms liquid sample flow channel with colloid, obtained card form potassium ion sensor.
On one side 10ul solution to be measured is instilled by the card form potassium ion sensor of preparation, while drip upper 10ul reference solution (in reference solution, potassium concentration is 8mM), with the electrode potential of homemade high input impedance amplifying circuit by the contact electrode measurement potassium ion sensor of two potassium ion-selective electrodes, record 40s.This potential value is relevant to the log-linear of potassium concentration in fluid to be measured, obtains response electromotive force thus, can extrapolate the potassium concentration in solution to be measured.
Fig. 3 is that (this solution is containing 1.0mMCaCl for the card form potassium ion sensor measurement variable concentrations KCl solution using this example to prepare
2with 140mMNaCl back-ground electolyte) the potential response calibration curve that obtains, correction equation is EMF (mV)=60.5lgC(mmol/L)+80.7, as shown in figure cathetus, EMF represents response electromotive force, C represents potassium concentration in solution, coefficient R
2be 99.97.
Fig. 4 is the potential response calibration curve that the card form potassium ion sensor using this example to prepare measures potassium concentration in serum, and its correction equation is EMF (mV)=62.5lgC(mmol/L)-36.7, as shown in figure cathetus, coefficient R
2be 99.76.
Can be found out by Fig. 3 and Fig. 4, card form potassium ion sensor response gradient prepared by this example meets Nernst equation.
The card form potassium ion sensor prepared by the present embodiment is preserved 60 days under normal temperature normality, detect the response gradient of every day, draw out response gradient relation over time, as shown in Figure 5, card form potassium ion sensor its response gradient in 60 days that prepared by this example remains on 60mV/-pK substantially
+, illustrate that the present invention has good job stability.
embodiment 2
The preparation method of a kind of card form potassium ion sensor of the present invention:
(1) silver-colored printing material is printed on polypropylene base plate by screen printing technique, forms the reaction electrode of potassium ion-selective electrode; Platinum printing material is printed on polypropylene base plate by screen printing technique, forms the conductive lead wire connected with reaction electrode and contact electrode;
(2) absolute ethyl alcohol is used to clean reaction electrode surface, then wiped clean;
(3) reaction electrode being dipped into mass percent is 20min in the liquor potassic permanganate of 15%, and effects on surface carries out oxidation processes, processes rear purified water and rinses well, dries in 80 DEG C of baking ovens;
(4) by dipotassium hydrogen phosphate (K
2hPO
4, Shanghai traditional Chinese medicines group) be dissolved in purified water and form the solution that concentration is 3.0mM concentration, until completely dissolved, add the xanthans (Shanghai traditional Chinese medicines group) that mass percent is 5%, fully stir evenly, form the dielectric substrate slurry of clear viscous, by the method for serigraphy, this dielectric substrate slurry is printed on the reaction electrode surface of potassium ion-selective electrode, form dielectric substrate, thickness is about 50um, and web plate used is 250 orders, and net is apart from 1.5mm, scraper velocity 15mm/s, pressure 2.5kg/cm
2;
(5) in 112.4ul dibutyl phthalate, 17.6mg a word used in person's names amino doxycycline, the tetraphenylboron sodium of 8.8mg and the PVC of 1600mg is added, shake after 1 minute, add 8ml cyclohexanone, concussion is dissolved and is obtained the potassium ion film forming liquid of homogeneous transparent thickness, by the method for serigraphy by the electrolyte layer surface of this potassium ion film forming liquid at potassium ion-selective electrode, form potassium ion sensitive membrane, thickness is about 10um, and web plate used is 150 orders, and net is apart from 1.5mm, scraper velocity 15mm/s, pressure 2.5kg/cm
2;
(6) method of serigraphy is adopted to print insulation course, insulation course surrounds potassium ion sensitive membrane, insulation course is provided with the circular open of two diameter 3mm, make potassium ion sensitive membrane exposed, as the conversion zone of potassium ion sensitive membrane, potassium ion sensitive membrane is contacted with solution to be measured, reference solution in measuring process by opening, and insulating layer material adopts the insulation dyestuff SS8391 that in precious magnificent cause, Co., Ltd produces;
(7) double-sided tape (3M) of two wide 0.5cm, thick 0.25mm is affixed on the both sides of insulation course upper shed, between two adhesive tape, forms the wide groove of 2mm;
(8) surface hydrophilicity cover plate is covered in colloid surface, forms liquid sample flow channel with colloid, obtained card form potassium ion sensor.
Fig. 6 is that (this solution is containing 1.0mMCaCl for the card form potassium ion sensor measurement variable concentrations KCl solution using the present embodiment to prepare
2with 140mMNaCl back-ground electolyte) the potential response calibration curve that obtains, correction equation is EMF (mV)=64.4lgC(mmol/L)+55.0, as shown in figure cathetus, EMF represents response electromotive force, C represents potassium concentration in solution, coefficient R
2be 99.98.
As seen from Figure 7, the card form potassium ion sensor response gradient that prepared by the present embodiment is slightly more than Nernst equation coefficient.
embodiment 3
The preparation method of card form potassium ion sensor, step is as follows:
(1) silver-colored printing material is printed on polypropylene base plate by screen printing technique, forms the reaction electrode of potassium ion-selective electrode; Platinum printing material is printed on polypropylene base plate by screen printing technique, forms the conductive lead wire connected with reaction electrode and contact electrode;
(2) absolute ethyl alcohol is used to clean reaction electrode surface, then wiped clean;
(3) reaction electrode being dipped into mass percent is 20min in the liquor potassic permanganate of 15%, and effects on surface carries out oxidation processes, processes rear purified water and rinses well, dries in 80 DEG C of baking ovens;
(4) by sal tartari (K
2cO
3, Shanghai traditional Chinese medicines group) be dissolved in purified water and form the solution that concentration is 4.5mM concentration, until completely dissolved, add the xanthans (Shanghai traditional Chinese medicines group) that mass percent is 5%, fully stir evenly, form the dielectric substrate slurry of clear viscous, by the method for serigraphy, this dielectric substrate slurry is printed on the reaction electrode surface of potassium ion-selective electrode, form dielectric substrate, thickness is about 50um, and web plate used is 250 orders, and net is apart from 1.5mm, scraper velocity 15mm/s, pressure 2.5kg/cm
2;
(5) in 2.2ml dibutyl sebacate, 18.9mg a word used in person's names amino doxycycline, the tetraphenylboron sodium of 17.0mg and the PVC of 1120mg is added, shake after 1 minute, add 8ml acetone, concussion is dissolved and is obtained the potassium ion film forming liquid of homogeneous transparent thickness, by the method for serigraphy by the electrolyte layer surface of this potassium ion film forming liquid at potassium ion-selective electrode, form potassium ion sensitive membrane, thickness is about 10um, and web plate used is 150 orders, and net is apart from 1.5mm, scraper velocity 15mm/s, pressure 2.5kg/cm
2;
(6) method of serigraphy is adopted to print insulation course, insulation course surrounds potassium ion sensitive membrane, insulation course is provided with the circular open of two diameter 3mm, make potassium ion sensitive membrane exposed, as the conversion zone of potassium ion sensitive membrane, potassium ion sensitive membrane is contacted with solution to be measured, reference solution in measuring process by opening, and insulating layer material adopts the insulation dyestuff SS8391 that in precious magnificent cause, Co., Ltd produces;
(7) double-sided tape (3M) of two wide 0.5cm, thick 0.25mm is affixed on the both sides of insulation course upper shed, between two adhesive tape, forms the wide groove of 2mm;
(8) surface hydrophilicity cover plate is covered in colloid surface, forms liquid sample flow channel with colloid, obtained card form potassium ion sensor.
As an alternative solution, potassium ion active carrier also can with being selected from the 4-tert-butyl group-2,2,14,14-tetra-with-2a, 14a, dioxy cup [4] aryl four acetic acid tetra-tert ester, two (phendioxin 5-crown ether-4)-4
,-ylmethyl pimelate, 2-dodecyl-2-methyl isophthalic acid, 3-glyceryl-bis--[N-(5
,-nitro (benzene-15-crown ether-5)-4
,base] one or more in carbamate.
One or more that in the present embodiment, the large molecule of lipophilicity also can be used in tetrachlorobenzene boron potassium, four [3,5-bis-(trifluoromethy) phenyl] potassium borate substitute.
The present embodiment non-conductive polymer polymkeric substance also can substitute by one or more in Polyvinylchloride, polyurethane, polyvinyl acetate, polymethylmethacrylate.
Plastifier used can substitute by one or more in ortho-nitrophenyl octyl ether, amyl phthalate, dioctyl phthalate.
Salt bridge one or more in following material optional in each embodiment: filter paper, cotton, chemical fibre, nonwoven fabrics, agar, gel.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (1)
1. a preparation method for card form potassium ion sensor, is characterized in that, comprises the following steps:
(1) on high-molecular organic material base plate, coated with conductive material forms reaction electrode, the contact electrode and conductive lead wire of potassium ion-selective electrode;
(2) oxidizing solution is used to carry out surface treatment to the reaction electrode of potassium ion-selective electrode; Wherein, oxidizing solution oxidation processes being carried out to reaction electrode surface to be mass percent be 15% ~ 25% potassium bichromate solution or liquor potassic permanganate;
(3) inorganic salts containing potassium ion are dissolved in purified water, be mixed with the solution that concentration is 0.5mM ~ 5.0mM, then in this solution, the xanthans that final mass number percent is 3.0% ~ 8.0% is added, be mixed with electrolyte slurry, this electrolyte slurry is coated in reaction electrode surface, forms dielectric substrate;
(4) potassium ion active carrier, the large molecule of lipophilicity, non-conductive polymer polymkeric substance, plasticizer dissolves are mixed with viscous solution in cyclohexanone or acetone, as potassium ion-selective electrode film liquid, in the gross mass of solution, wherein the mass percent of potassium ion active carrier is 0.2% ~ 0.5%, the macromolecular mass percent of lipophilicity is 0.5% ~ 1.0%, the mass percent of non-conductive polymer polymkeric substance is 30.0% ~ 35.0%, the mass percent of plastifier is 8.0% ~ 14.0%, remaining as solvent; Electrode film liquid is coated in the electrolyte layer surface of potassium ion-selective electrode, forms potassium ion sensitive membrane, lucifuge freeze-drying lyophilization is preserved;
(5) on base plate, print insulation course, insulation course covers potassium ion sensitive membrane, and insulation course is provided with the opening making potassium ion sensitive membrane exposed, the conversion zone that when this open area is actual measurement, potassium ion sensitive membrane and solution to be measured come in contact;
(6) two double faced adhesive tape colloids are affixed on respectively the both sides of insulation course upper shed, the length of colloid enough connects two potassium ion-selective electrodes, forms groove between two colloids;
(7) cover plate of surface hydrophilicity is covered in colloid surface, i.e. obtained card form potassium ion sensor;
Wherein, the described inorganic salts containing potassium ion are selected from one or more in sal tartari, dipotassium hydrogen phosphate, potassium sulfate; Described potassium ion active carrier is selected from a word used in person's names amino doxycycline, the 4-tert-butyl group-2,2,14,14-tetra-is with-2a, 14a, dioxy cup [4] aryl four acetic acid tetra-tert ester, two (phendioxin 5-crown ether-4)-4 '-ylmethyl pimelate, 2-dodecyl-2-methyl isophthalic acid, one or more in 3-glyceryl-bis--[N-(5 '-nitro (benzene-15-crown ether-5)-4 ' base] carbamate; The large molecule of described lipophilicity is selected from one or more in tetraphenylboron sodium, tetrachlorobenzene boron potassium, four [3,5-bis-(trifluoromethy) phenyl] potassium borate; Described non-conductive polymer polymkeric substance is selected from one or more in Polyvinylchloride, polyurethane, polyvinyl acetate, polymethylmethacrylate; Described plastifier is selected from one or more in ortho-nitrophenyl octyl ether, amyl phthalate, dioctyl phthalate, di-n-octyl sebacate, dibutyl sebacate.
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| CN101871912A (en) * | 2010-06-07 | 2010-10-27 | 浙江大学 | Full-solid potassium ion sensor and preparation method thereof |
| CN102636532A (en) * | 2012-04-24 | 2012-08-15 | 南京工业大学 | Medical all-solid-state potassium ion selective sensor and preparation method thereof |
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