CN107271521A - A kind of potassium ion biochemical analysis dry plate and preparation method thereof and reagent disc - Google Patents
A kind of potassium ion biochemical analysis dry plate and preparation method thereof and reagent disc Download PDFInfo
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- CN107271521A CN107271521A CN201610219139.4A CN201610219139A CN107271521A CN 107271521 A CN107271521 A CN 107271521A CN 201610219139 A CN201610219139 A CN 201610219139A CN 107271521 A CN107271521 A CN 107271521A
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Abstract
The invention discloses a kind of potassium ion biochemical analysis dry plate and preparation method thereof and reagent disc.Wherein potassium ion biochemical analysis dry plate includes：Substrate, a pair of potassium ion-selective electrodes, insulating barrier and salt bridge；Potassium ion-selective electrode includes successively from bottom to up：Contact electrode layer, reaction electrode layer, electrolyte reference layer and potassium ion selective membrane；Electrode layer segment is contacted to be covered by reaction electrode layer；Potassium ion-selective electrode is located on substrate and separation is arranged side by side；Covered with insulating barrier on potassium ion-selective electrode；Salt bridge is sealed in insulating barrier, and through hole is had on salt bridge and insulating barrier position corresponding with a pair of potassium ion-selective electrodes；On insulating barrier electrode contact hole is had with contacting electrode layer unmasked portion correspondence position.Potassium ion biochemical analysis dry plate is dry chemistry reagent, and without liquid, the article needed for total Test is included in a piece of dryness reagent, it is easy to preserves and carries, required sample size is less, can guarantee that the accuracy of testing result.
Description
Technical field
The present invention relates to medical detection field, more particularly to a kind of potassium ion biochemical analysis dry plate and its preparation side
Method and reagent disc.
Background technology
Potassium ion is as indispensable macroelement in human body, and potassium concentration is about in general adult
3.5~5.5mmol/L.Main function of the potassium in human body is to maintain acid-base balance, participates in energetic supersession and dimension
Hold nervimuscular normal function.When internal potassium deficiency, energy and physically-draining, temperature capacity drop can be caused
It is low, make one to feel powerless；During severe potassium deficiency, acid-base imbalance in human body, metabolic disorder, the heart can be caused
Rule is not normal, even results in respiratory paralysis dead.Therefore, in the development of present medical science, particularly in clinic
In the rescue of urgent patient, the detection of potassium ion is one of important electrolyte Testing index, it with cardiac muscle
The important physiological functions such as electrophysiologic activity, the contractile function of skeletal muscle, acid-base balance have substantial connection.
Potassium concentration uses potassium ion-selective electrode in detection blood at present.
Traditional potassium ion-selective electrode is by internal reference electrode, interior reference solution and sensitive membrane composition.During measurement,
Potassium ion-selective electrode and reference electrode are constituted into potassium ion sensor, and structure is connected with external measurement apparatus
Into an electrochemical cell, the electrode potential of the electromotive force and reference electrode, internal reference electrode of the battery is met
Following Nernst equation：
Wherein, R is mol gas constant, and T is thermodynamic temperature, and Z is the valence state for treating measured ion, and F is
Faraday constant.A prepare liquids are the ion concentration in solution to be measured, a titers for standard reference liquid from
Sub- concentration.The ion concentration that the electrode potential of reference electrode is generally in definite value, standard liquid be also it is known,
Then by measuring cell emf, it is possible to calculate the ion concentration for surveying solution.
The interior reference solution of conventional ion electrodes selective typically uses electrolyte solution, can only be in relatively low temperature
Degree, work under pressure, it is made, and this is higher, and causes the volume of potassium ion-selective electrode larger, causes
Low-response, it is difficult to carry out the miniaturization of electrode, working service complexity cost is high, and each to avoid when using
Cleaning is required for when the infection of sample is measured every time, use time length just result error occurs, it is necessary to frequency
Numerous calibration, complex operation is not portable, it is impossible to meet the demand of the occasions such as operation, emergency treatment, field rescue,
Also domestic medicine, the trend of modern medical service in-vitro diagnosis apparatus pattern can not be adapted to.
The content of the invention
In view of the above problems, it is proposed that the present invention overcomes above mentioned problem or at least in part to provide one kind
A kind of potassium ion biochemical analysis dry plate solved the above problems and preparation method thereof and reagent disc.
In a first aspect, the embodiment of the present invention provides a kind of potassium ion biochemical analysis dry plate, including：Substrate, one
To potassium ion-selective electrode, insulating barrier and salt bridge；Wherein：
The potassium ion-selective electrode includes successively from bottom to up：Contact electrode layer, reaction electrode layer, electrolysis
Matter reference layer and potassium ion selective membrane；Wherein described contact electrode layer segment is covered by reaction electrode layer；
The pair of potassium ion-selective electrode is located on the substrate, and separation arrangement side by side；It is the pair of
Covered with the insulating barrier on potassium ion-selective electrode；
The salt bridge is sealed among the insulating barrier, and the salt bridge and insulating barrier are selected with the pair of potassium ion
Select and through hole is had on the corresponding position of electrode, form reference liquid and the reaction chamber of test fluid；
Electrode layer unmasked portion is contacted with the pair of potassium ion-selective electrode on the insulating barrier corresponding
Position has electrode contact hole.
In one embodiment, the material of the contact electrode layer is gold, silver, platinum or carbon；The reaction electricity
The material of pole is：Gold, platinum, carbon or silver chlorate.
In one embodiment, the electrolyte reference layer includes：Hydrophilic adhesive, surfactant,
Bactericidal agent, dispersant, potassium chloride and sodium chloride.
In one embodiment, in the electrolyte reference layer：Hydrophilic adhesive is that electrolyte reference layer is total
The 50%~70% of mass fraction；Surfactant is the 0.1%~3% of electrolyte reference layer total mass fraction；
Bactericidal agent is the 0.5%~2% of electrolyte reference layer total mass fraction；Dispersant is electrolyte reference layer gross mass
The 5%~12% of fraction；Potassium chloride is the 6%~13% of total mass fraction；Sodium chloride is total mass fraction
20%~35%.
In one embodiment, the hydrophilic adhesive is polyvinyl alcohol, polyacrylate and carboxylation polychlorostyrene
One or more in ethene；The dispersant is glycerine.
In one embodiment, the potassium ion selective membrane includes：Non-conductive polymer polymer, lipophilicity
Macromolecular compound, carrier, carrier solvent and surfactant.
In one embodiment, in the potassium ion selective membrane：Non-conductive polymer polymer volume be potassium from
The 15%~35% of sub- selective membrane total mass fraction, lipophilicity macromolecular compound consumption is potassium ion selective membrane
The 0.5%~2% of total mass fraction, carrier consumption is the 1%~5% of potassium ion selective membrane total mass fraction, is carried
Body solvent load be potassium ion selective membrane total mass fraction 40%~80%, dosage of surfactant be potassium from
The 0.1%~3% of sub- selective membrane total mass fraction.
In one embodiment, non-conductive polymer polymer includes：It is polyacrylate, polyvinyl chloride, poly-
One in urethane, polyvinyl alcohol, polystyrolsulfon acid, polymethyl methacrylate, hydroxyvinyl resin
Plant or several；
Lipophilicity macromolecular compound includes：Tetraphenylboron sodium, tetrachloro phenylboric acid potassium, the four [(trifluoros of 3,5- bis-
Methyl) phenyl] one or more in potassium borate；
Carrier is valinomycins, double [(benzene -15- crown ethers -4) -4- methyl] pimelates, 2- dodecane -2- methyl
- 1,3- glyceryl it is double [N- (5- nitros (benzene -15- crown ethers -5) -4- bases) carbamate, dibenzo-18-crown-6 (DB18C6) or
Any of bicyclohexane and 24- crown ethers -8；
Carrier solvent includes：Phthalate, sebacic acid ester, ortho-nitrophenyl octyl ether, 2- ethyl hexyls
The one or more such as ester；
Surfactant includes：Organosilicon alkanes surfactant or siloxane type surfactants.
In one embodiment, the salt bridge is filter paper；
The substrate uses glass, polypropylene, polyvinyl chloride, polyethylene or polyester；
The insulating barrier uses polypropylene, polyvinyl chloride, polyethylene or polyester.
In one embodiment, the insulating barrier edge and the substrate edges are bonded by adhesive.
Second aspect, the embodiment of the present invention, which is provided on a kind of reagent disc, the reagent disc, at least one foregoing potassium
Ion biochemical analysis dry plate.
The third aspect, the embodiment of the present invention provides a kind of preparation method of potassium ion biochemical analysis dry plate, including：
Prepare contact electrode layer, reaction electrode layer, electrolyte reference layer and potassium ion selection respectively on substrate
Film, forms the potassium ion-selective electrode of pair of parellel separation arrangement；The contact electrode layer segment is by described anti-
Electrode layer is answered to cover；
Salt bridge is sealed in insulating materials, the insulating barrier for being sealed with salt bridge is formed；Beaten in the insulating barrier
Two are touched through the insulating barrier and the through hole of the salt bridge, and two electrodes through the insulating barrier
Hole；
The insulating barrier for being sealed with salt bridge is covered on a pair of potassium ion-selective electrodes and is bonded thereto；It is described
The potassium ion selective membrane part of a pair of potassium ion-selective electrodes is corresponding and described through hole site with two respectively
Electrode layer unmasked portion is contacted in potassium ion-selective electrode corresponding with two electrode contact hole positions；
Insulating barrier edge is Nian Jie with substrate edges.
In one embodiment, it is above-mentioned to prepare contact electrode layer, reaction electrode layer, electrolysis respectively on substrate
Matter reference layer and potassium ion selective membrane, the step of forming the potassium ion-selective electrode of pair of parellel separation arrangement,
It can include：
Conducting metal is adhered to by silk-screen printing, vacuum coating or plating mode on substrate, one is prepared respectively
To the contact electrode layer of potassium ion-selective electrode；
By way of oxidation or silk-screen printing reaction electrode layer is prepared on the contact electrode layer；
Electrolyte solution is coated on the reaction electrode layer above and dried, electrolyte reference layer is prepared；
Non-conductive polymer polymer, lipophilicity macromolecular compound, carrier, carrier solvent, surface are lived
Property agent be dissolved in organic solvent, it is upper and dry to be coated on electrolyte reference layer, prepares potassium ion selection
Film.
The beneficial effect of above-mentioned technical proposal provided in an embodiment of the present invention at least includes：
The technology of potassium ion biochemical analysis dry plate provided in an embodiment of the present invention and preparation method thereof and reagent disc
In scheme, potassium ion biochemical analysis dry plate is the dry chemistry reagent of multilayer film, without liquid, total Test
Required article is included in a piece of dryness reagent, is monitored using dry ion-selective electrodes, will be joined
Solid electrolyte reference layer is changed into than liquid, detects that the simple to operate, time is short, it is easy to preserves and carries, and
And stabilization time is longer, required specimen amount is less, meanwhile, traditional biochemical reaction method is remained again,
The chemical reaction of detection is identical with traditional biochemical pattern or principle, improves the traceability of system, it is ensured that
The accuracy of testing result.Also, above-mentioned technical proposal make it that the detection using this biochemical analysis dry plate is set
Standby to realize miniaturization, raising portability can meet operation, emergency first-aid, field rescue, family and examine
Treatment etc. needs the urgent various occasions for rapidly and accurately obtaining assay.
Also, due to that need not be used in the embodiment of the present invention with the pairing of outer reference electrode, two pairs of potassium ion choosings
Select that electrode is identical, the reaction chamber of test fluid and reference liquid can unrestricted choice.And in preparation technology in electrode
Reference liquid and test fluid is contacted with each other using the osmosis of salt bridge, make two potassium ion-selective electrode connections,
Loop is formed, preparation technology is simple, it is adaptable to industrialized production.
Other features and advantages of the present invention will be illustrated in the following description, also, partly from explanation
Become apparent, or understood by implementing the present invention in book.The purpose of the present invention and other advantages can
Realize and obtain by specifically noted structure in the specification, claims and accompanying drawing write
.
Below by drawings and examples, technical scheme is described in further detail.
Brief description of the drawings
Accompanying drawing is used for providing a further understanding of the present invention, and constitutes a part for specification, with this hair
Bright embodiment is used to explain the present invention together, is not construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the structural representation of the potassium ion biochemical analysis dry plate provided in the embodiment of the present invention.
Fig. 2 is the hierarchy schematic diagram of potassium ion-selective electrode provided in an embodiment of the present invention；
Fig. 3 is the KNO that potassium ion biochemical analysis dry plate provided in an embodiment of the present invention tests various concentrations3It is molten
The curve map of the potassium concentration that liquid is obtained and response potential；
Fig. 4 is the characteristic curve of potassium ion biochemical analysis dry plate provided in an embodiment of the present invention.
Embodiment
The exemplary embodiment of the disclosure is more fully described below with reference to accompanying drawings.Although being shown in accompanying drawing
The exemplary embodiment of the disclosure, it being understood, however, that may be realized in various forms the disclosure without should be by
Embodiments set forth here is limited.It is opposite to be able to be best understood from this there is provided these embodiments
It is open, and can by the scope of the present disclosure completely convey to those skilled in the art.
It is shown referring to Figures 1 and 2 the embodiments of the invention provide a kind of potassium ion biochemical analysis dry plate, bag
Include：Substrate 1, a pair of potassium ion-selective electrodes 2, insulating barrier 3 and salt bridge 4；Wherein：
Potassium ion-selective electrode 2 includes successively from bottom to up：Contact electrode layer 21, reaction electrode layer 22,
Electrolyte reference layer 23 and potassium ion selective membrane 24；Wherein the contact part of electrode layer 21 is by reaction electrode layer
22 coverings；
A pair of potassium ion-selective electrodes 2 are located on substrate 1, and separation arrangement side by side；A pair of potassium ion choosings
Select on electrode 2 covered with insulating barrier 3；
Salt bridge 4 is sealed among insulating barrier 3, salt bridge 4 and insulating barrier 3 and a pair of potassium ion-selective electrodes 2
Through hole 31 is had on corresponding position, reference liquid and the reaction chamber of test fluid is formed；
The corresponding position of the unmasked portion of electrode layer 21 is contacted on insulating barrier 3 with a pair of potassium ion-selective electrodes 2
Put and have electrode contact hole 32.
Electrode contact hole 32 is corresponding with contacting electrode layer position, so, the electrode tip of detecting instrument by this two
Individual electrode contact hole 32 can just be contacted with contacting electrode layer, so as to read the response potential of the biochemical dry plate of potassium ion.
Substrate 1 is sealing, the part of protection potassium ion-selective electrode, can use glass, various macromolecule materials
Expect such as polypropylene, polyvinyl chloride, polyethylene or polyester.
The material of insulating barrier 3 can use various non-conductive polymer materials, for example polypropylene, polyvinyl chloride,
Polyethylene or polyester.
The material for contacting electrode layer 21 is gold, silver, platinum or carbon；The material of reaction electrode is：Gold, platinum,
Carbon or silver chlorate.
For example, contact electrode layer 21 is conductive metal such as argent, reaction electrode can use silver chlorate.
In one embodiment, above-mentioned electrolyte reference layer 23 includes：Hydrophilic adhesive, surface-active
Agent, bactericidal agent, dispersant, potassium chloride and sodium chloride.
In one embodiment, the quality accounting of each component is as follows in electrolyte reference layer 23：
Hydrophilic adhesive (for example can be one in polyvinyl alcohol, polyacrylate and carboxylation polyvinyl chloride
Plant or several) it is the 50%~70% of electrolyte reference layer total mass fraction；
Surfactant (organosilicon alkanes surfactant or siloxane type surfactants etc. can be used) for
The 0.1%~3% of electrolyte reference layer total mass fraction；
Bactericidal agent is the 0.5%~2% of electrolyte reference layer total mass fraction；
Dispersant (such as can use glycerine) is the 5%~12% of electrolyte reference layer total mass fraction；
Potassium chloride is the 6%~13% of total mass fraction；Sodium chloride is the 20%~35% of total mass fraction.
Potassium chloride is the 6%~13% of total mass fraction；Sodium chloride is the 20%~35% of total mass fraction.
In one embodiment, potassium ion selective membrane 24 includes：Non-conductive polymer polymer, lipophilicity
Macromolecular compound, carrier, carrier solvent and surfactant.
In one embodiment, the quality accounting of each component is as follows in potassium ion selective membrane 24：
Non-conductive polymer polymer volume is the 15%~35% of potassium ion selective membrane total mass fraction, lipophilic
Property macromolecular compound consumption be the 0.5%~2% of potassium ion selective membrane total mass fraction, carrier consumption be potassium from
The 1%~5% of sub- selective membrane total mass fraction, carrier solvent consumption is potassium ion selective membrane total mass fraction
40%~80%, dosage of surfactant is the 0.1%~3% of potassium ion selective membrane total mass fraction.
Above-mentioned non-conductive polymer polymer can include：It is polyacrylate, polyvinyl chloride, polyurethane, poly-
One or more in vinyl alcohol, polystyrolsulfon acid, polymethyl methacrylate, hydroxyvinyl resin；
Wherein, the hydrophily of ion-selective membrane can be increased using hydroxyvinyl resin, when shortening the response of electrode
Between be conducive to electrode potential response stabilization.Pendant hydroxyl group advantageously reduces the use of plasticizer, can subtract
Few carrier is lost in, and increases detection range and the life-span of ion-selective membrane.
Lipophilicity macromolecular compound can include：Tetraphenylboron sodium, tetrachloro phenylboric acid potassium, four [3,5- bis- (three
Methyl fluoride) phenyl] one or more in potassium borate；
Carrier can be valinomycins, double [(benzene -15- crown ethers -4) -4- methyl] pimelates, 2- dodecanes -2-
Methyl isophthalic acid, 3- glyceryl double [N- (5- nitros (benzene -15- crown ethers -5) -4- bases) carbamate, dibenzo -18- crown ethers
Any of -6 or bicyclohexane and 24- crown ethers -8；
Carrier solvent can include：Phthalate, sebacic acid ester, ortho-nitrophenyl octyl ether, 2- second
The one or more such as the own ester of base；
Surfactant includes：Organosilicon alkanes surfactant or siloxane type surfactants.
In one embodiment, salt bridge is filter paper.
In order to ensure exchange velocity, it is to avoid exchange is too fast to be caused the unstable of test signal and then cause test to be tied
It is really inaccurate, Medium speed filter paper can be used, it is ensured that fluid exchange speed is not too fast or excessively slow.
It is added dropwise in the reactor chamber among reference liquid and test fluid, the liquid on both sides passes through salt bridge mutual conduction.
In one embodiment, insulating barrier edge and substrate edges are bonded by adhesive.
Adhesive can using crylic acid resin, epoxy resin, Corvic class, polyurethanes and
The adhesives such as silicon rubber.
The embodiment of the present invention additionally provides a kind of reagent disc, there is at least one above-mentioned potassium ion life on the reagent disc
Change analysis dry plate.
The skill of above-mentioned potassium ion biochemical analysis dry plate and the reagent disc for being provided with the potassium ion biochemical analysis dry plate
In art scheme, potassium ion biochemical analysis dry plate is the dry chemistry reagent of multilayer film, without liquid, all examination
Article needed for testing is included in a piece of dryness reagent, is monitored using dry ion-selective electrodes, will
Reference liquid changes into solid electrolyte reference layer, detects that the simple to operate, time is short, it is easy to preserves and carries,
And stabilization time is longer, required specimen amount is less, meanwhile, traditional biochemical reaction method is remained again,
The chemical reaction of detection is identical with traditional biochemical pattern or principle, improves the traceability of system, it is ensured that
The accuracy of testing result.Feature so that given birth to using potassium ion biochemical analysis dry plate with the potassium ion is provided with
The detector for changing the reagent disc of analysis dry plate can be simplified without the pure water that is needed to use using biochemistry
The detection process of traditional detection instrument, simultaneously as the These characteristics of potassium ion biochemical analysis dry plate so that make
It can be minimized, be easy to carry while testing result accuracy is ensured with the detector of the reagent disc, from
And shorten the time expended from sampling, sample presentation and detection whole process, operation, emergency first-aid, open country can be met
Outer rescue, family's diagnosis and treatment etc. need the urgent various occasions for rapidly and accurately obtaining assay.
The embodiment of the present invention additionally provides a kind of preparation method of potassium ion biochemical analysis dry plate, including following steps
Suddenly：
S1, prepare contact electrode layer, reaction electrode layer, electrolyte reference layer and potassium ion respectively on substrate
Selective membrane, forms the potassium ion-selective electrode of pair of parellel separation arrangement；Electrode layer segment is contacted by described anti-
Electrode layer is answered to cover；
S2, salt bridge is sealed in insulating materials, forms the insulating barrier for being sealed with salt bridge；In the insulating barrier
In make a call to two through holes through the insulating barrier and the salt bridge, and two electricity for running through the insulating barrier
Pole contact hole；
S3, the insulating barrier for being sealed with salt bridge is covered on a pair of potassium ion-selective electrodes and be bonded thereto；
The potassium ion selective membrane part of the pair of potassium ion-selective electrode is corresponding through hole site with two respectively, and
Electrode layer unmasked portion is contacted in the potassium ion-selective electrode corresponding with two electrode contact hole positions；
It is S4, insulating barrier edge is Nian Jie with substrate edges.
Wherein, above-mentioned steps S1, in the specific implementation, can comprise the steps：
S11, on substrate pass through silk-screen printing, vacuum coating or plating mode adhere to conducting metal, respectively
Prepare the contact electrode layer of a pair of potassium ion-selective electrodes；
S12, oxidation or silk-screen printing by way of it is described contact electrode layer on prepare reaction electrode layer；
S13, that electrolyte solution is coated on to reaction electrode layer is upper and dry, and prepares electrolyte reference layer；
S14, by non-conductive polymer polymer, lipophilicity macromolecular compound, carrier, carrier solvent,
Surfactant is dissolved in organic solvent, and it is upper and dry to be coated on electrolyte reference layer, prepare potassium from
Sub- selective membrane.
Illustrate the preparation method of above-mentioned potassium ion biochemical analysis dry plate with a specific example below.
In this example embodiment, potassium ion biochemical analysis dry plate is prepared, can be comprised the steps：
S1 ', on polyethylene terephthalate with silk-screen printing technique in its surface printing silver paste, system
Standby contact electrode layer 21.Printing equipment half tone is 200 mesh, 200 μm of print thickness, and condition of cure is：150 DEG C,
30min。
S2 ', by it is dried contact electrode layer it is preceding 2/3 section using chemical method carry out aoxidize obtain reaction electricity
Pole layer 22.
Specific oxidation step：The contact electrode for needing to aoxidize position is immersed in 0.1~1mol/L ferric chloride solutions
Or after mass fraction is 60s~150s in 10%~20% liquor potassic permanganate, taking-up is cleaned dry with pure water
It is net and dry, that is, obtain reaction electrode layer 22.
S3 ', electrolyte reference layer coating.By 6g polyvinyl alcohol, 1g glycerine, 3g sodium chloride, 1g
Potassium chloride, 0.02g bactericidal agents, 0.004g surfactants are dissolved in 50g water, will with automatic coating machine
It is coated on reaction electrode layer 22,200 μm~400 μm of coating thickness, coating speed 50mm/s, instead
18~30 DEG C, the ring of relative humidity 40%~60% are positioned over after answering the electrolyte reference layer after the coating of electrode layer 22
40~80min is dried under border.
By adjusting the proportioning of sodium chloride and potassium chloride, dried using fixed electrolyte reference layer and coating work
Skill, the water content of control electrolyte reference layer, it is ensured that electrolyte reference layer intermediate ion solubility is stable and is electrolysed
The adhesive capacity of matter reference layer and ion-selective membrane, the stability of lifting ion-selective electrode signal response.
S4 ', potassium ion selective membrane coating.By 0.1g hydroxyvinyl resins, 0.1g ortho-nitrophenyl octyl ethers,
Double [(benzene -15- crown ethers-the 4) -4- methyl] pimelates, 0.002g tetraphenylboron sodiums, 0.005g surface-actives of 0.005g
It is (a kind of or many such as acetone, tetrahydrofuran, dichloromethane, dimethylformamide that agent is dissolved in organic solvent
Kind) in, it is coated on automatic coating machine on solid electrolyte reference layer 23, coating thickness
100 μm~300 μm, coating speed 50mm/s, and drying at room temperature.
S5 ', potassium ion selective membrane dry after the completion of, take two panels potassium ion-selective electrode 2 respectively with insulating barrier
3 and the through hole aligned in position on salt bridge 4 and with 705 silicon rubber be bonded.
After S6 ', adhesive solidification, by the edge of substrate 1 and the glue sticking of 3 edge of insulating barrier 705, adhesive
After solidification prepared by potassium ion biochemical analysis dry plate to complete.
The long-term storage conditions of above-mentioned potassium ion biochemical analysis dry plate provided in an embodiment of the present invention are：
- 10~-18 DEG C.
Reuse, protected during test after 1h in use, needing to place at normal temperatures when taking out under low temperature environment
Hold 37 ± 0.1 DEG C of test temperature.During test, in the reaction chamber of potassium ion biochemical analysis dry plate, (two run through
Hole) in be added dropwise reference liquid and test fluid respectively, the required μ L of sample 10~20, the testing time is less than 50s.
Shown in reference picture 3, test different dense using potassium ion biochemical analysis dry plate provided in an embodiment of the present invention
The KNO of degree3The response potential test result of solution (0.001mol/L, 0.004mol/L, 0.07mol/L)
Figure.In Fig. 3, Y represents to respond potential, and X represents that logC, C are potassium concentration in solution.
Potassium ion biochemical analysis dry plate is calibrated before test, passes through known potassium ion solubility titer (potassium
Ion solubility 2mmol/L and 11mmol/L), the response potential of potassium ion biochemical analysis dry plate is tested, is obtained
Potential response corrections curve.Correction equation is：Y=60.324X+53.468.Y represents to respond potential, X tables
It is potassium concentration in solution to show logC, C.The characteristic curve reference of obtained potassium ion biochemical analysis dry plate
Shown in Fig. 4.The response potential obtained according to the characteristic curve and test, it is possible to obtain the potassium of test sample
Ion concentration.
Obviously, those skilled in the art can carry out various changes and modification without departing from this hair to the present invention
Bright spirit and scope.So, if the present invention these modifications and variations belong to the claims in the present invention and
Within the scope of its equivalent technologies, then the present invention is also intended to comprising including these changes and modification.
Claims (13)
1. a kind of potassium ion biochemical analysis dry plate, it is characterised in that including：Substrate, a pair of potassium ion choosings
Select electrode, insulating barrier and salt bridge；Wherein：
The potassium ion-selective electrode includes successively from bottom to up：Contact electrode layer, reaction electrode layer, electrolysis
Matter reference layer and potassium ion selective membrane；Wherein described contact electrode layer segment is covered by reaction electrode layer；
The pair of potassium ion-selective electrode is located on the substrate, and separation arrangement side by side；It is the pair of
Covered with the insulating barrier on potassium ion-selective electrode；
The salt bridge is sealed among the insulating barrier, and the salt bridge and insulating barrier are selected with the pair of potassium ion
Select and through hole is had on the corresponding position of electrode, form reference liquid and the reaction chamber of test fluid；
Electrode layer unmasked portion is contacted with the pair of potassium ion-selective electrode on the insulating barrier corresponding
Position has electrode contact hole.
2. potassium ion biochemical analysis dry plate as claimed in claim 1, it is characterised in that the contact electricity
The material of pole layer is gold, silver, platinum or carbon；The material of the reaction electrode is：Gold, platinum, carbon or silver chlorate.
3. potassium ion biochemical analysis dry plate as claimed in claim 1, it is characterised in that the electrolyte
Reference layer includes：Hydrophilic adhesive, surfactant, bactericidal agent, dispersant, potassium chloride and sodium chloride.
4. potassium ion biochemical analysis dry plate as claimed in claim 1, it is characterised in that the electrolyte
In reference layer：Hydrophilic adhesive is the 50%~70% of electrolyte reference layer total mass fraction；Surface-active
Agent is the 0.1%~3% of electrolyte reference layer total mass fraction；Bactericidal agent is electrolyte reference layer total mass fraction
0.5%~2%；Dispersant is the 5%~12% of electrolyte reference layer total mass fraction；Potassium chloride is gross mass
The 6%~13% of fraction；Sodium chloride is the 20%~35% of total mass fraction.
5. potassium ion biochemical analysis dry plate as claimed in claim 4, it is characterised in that the hydrophily
Bonding agent is the one or more in polyvinyl alcohol, polyacrylate and carboxylation polyvinyl chloride；The dispersant
For glycerine.
6. potassium ion biochemical analysis dry plate as claimed in claim 1, it is characterised in that the potassium ion
Selective membrane includes：Non-conductive polymer polymer, lipophilicity macromolecular compound, carrier, carrier solvent and
Surfactant.
7. potassium ion biochemical analysis dry plate as claimed in claim 6, it is characterised in that the potassium ion
In selective membrane：Non-conductive polymer polymer volume is the 15%~35% of potassium ion selective membrane total mass fraction,
Lipophilicity macromolecular compound consumption is the 0.5%~2% of potassium ion selective membrane total mass fraction, and carrier consumption is
The 1%~5% of potassium ion selective membrane total mass fraction, carrier solvent consumption is potassium ion selective membrane gross mass point
Several 40%~80%, dosage of surfactant is the 0.1%~3% of potassium ion selective membrane total mass fraction.
8. potassium ion biochemical analysis dry plate as claimed in claims 6 or 7, it is characterised in that non-conductive
High molecular polymer includes：Polyacrylate, polyvinyl chloride, polyurethane, polyvinyl alcohol, polystyrene sulphur
One or more in acid, polymethyl methacrylate, hydroxyvinyl resin；
Lipophilicity macromolecular compound includes：Tetraphenylboron sodium, tetrachloro phenylboric acid potassium, the four [(trifluoros of 3,5- bis-
Methyl) phenyl] one or more in potassium borate；
Carrier is valinomycins, double [(benzene -15- crown ethers -4) -4- methyl] pimelates, 2- dodecane -2- methyl
- 1,3- glyceryl it is double [N- (5- nitros (benzene -15- crown ethers -5) -4- bases) carbamate, dibenzo-18-crown-6 (DB18C6) or
Any of bicyclohexane and 24- crown ethers -8；
Carrier solvent includes：Phthalate, sebacic acid ester, ortho-nitrophenyl octyl ether, 2- ethyl hexyls
The one or more such as ester；
Surfactant includes：Organosilicon alkanes surfactant or siloxane type surfactants.
9. potassium ion biochemical analysis dry plate as claimed in claim 1, it is characterised in that the salt bridge is
Filter paper；
The substrate uses glass, polypropylene, polyvinyl chloride, polyethylene or polyester；
The insulating barrier uses polypropylene, polyvinyl chloride, polyethylene or polyester.
10. the potassium ion biochemical analysis dry plate as described in claim 1-7 and 9 any one, it is characterised in that
The insulating barrier edge and the substrate edges are bonded by adhesive.
11. a kind of reagent disc, it is characterised in that included in the reagent disc such as any one of claim 1-10
Described potassium ion biochemical analysis dry plate.
12. a kind of preparation method of potassium ion biochemical analysis dry plate, it is characterised in that including：
Prepare contact electrode layer, reaction electrode layer, electrolyte reference layer and potassium ion selection respectively on substrate
Film, forms the potassium ion-selective electrode of pair of parellel separation arrangement；The contact electrode layer segment is by described anti-
Electrode layer is answered to cover；
Salt bridge is sealed in insulating materials, the insulating barrier for being sealed with salt bridge is formed；Beaten in the insulating barrier
Two are touched through the insulating barrier and the through hole of the salt bridge, and two electrodes through the insulating barrier
Hole；
The insulating barrier for being sealed with salt bridge is covered on a pair of potassium ion-selective electrodes and is bonded thereto；It is described
The potassium ion selective membrane part of a pair of potassium ion-selective electrodes is corresponding and described through hole site with two respectively
Electrode layer unmasked portion is contacted in potassium ion-selective electrode corresponding with two electrode contact hole positions；
Insulating barrier edge is Nian Jie with substrate edges.
13. the preparation method of potassium ion biochemical analysis dry plate as claimed in claim 12, it is characterised in that
Prepare contact electrode layer, reaction electrode layer, electrolyte reference layer and potassium ion selective membrane respectively on substrate,
The potassium ion-selective electrode of pair of parellel separation arrangement is formed, including：
Conducting metal is adhered to by silk-screen printing, vacuum coating or plating mode on substrate, one is prepared respectively
To the contact electrode layer of potassium ion-selective electrode；
By way of oxidation or silk-screen printing reaction electrode layer is prepared on the contact electrode layer；
Electrolyte solution is coated on the reaction electrode layer above and dried, electrolyte reference layer is prepared；
Non-conductive polymer polymer, lipophilicity macromolecular compound, carrier, carrier solvent, surface are lived
Property agent be dissolved in organic solvent, it is upper and dry to be coated on electrolyte reference layer, prepares potassium ion selection
Film.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610219139.4A CN107271521A (en) | 2016-04-08 | 2016-04-08 | A kind of potassium ion biochemical analysis dry plate and preparation method thereof and reagent disc |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610219139.4A CN107271521A (en) | 2016-04-08 | 2016-04-08 | A kind of potassium ion biochemical analysis dry plate and preparation method thereof and reagent disc |
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| CN201610219139.4A Pending CN107271521A (en) | 2016-04-08 | 2016-04-08 | A kind of potassium ion biochemical analysis dry plate and preparation method thereof and reagent disc |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732232A (en) * | 2018-04-19 | 2018-11-02 | 山西农业大学 | Exempt from the micro potassium content electrochemical detection method of electrode modification |
| CN113702465A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Potassium ion quantitative detection dry tablet for in vitro diagnosis and preparation method thereof |
| CN113702467A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Calcium ion quantitative detection dry tablet and preparation method thereof |
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| CN105223252A (en) * | 2015-10-23 | 2016-01-06 | 吴国清 | A kind of sodium, potassium, chlorion biochemical analysis dry plate and preparation method thereof |
| CN105301078A (en) * | 2015-10-23 | 2016-02-03 | 吴国清 | Reference layer used for solid ion-selective electrode and preparation method of reference layer |
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| US5021140A (en) * | 1988-01-13 | 1991-06-04 | Fuji Photo Film Co., Ltd. | Ion-selective electrode |
| CN102636532A (en) * | 2012-04-24 | 2012-08-15 | 南京工业大学 | Medical type all-solid potassium ion selectivity sensor and preparation method thereof |
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| CN108732232A (en) * | 2018-04-19 | 2018-11-02 | 山西农业大学 | Exempt from the micro potassium content electrochemical detection method of electrode modification |
| CN113702465A (en) * | 2020-05-20 | 2021-11-26 | 无锡锦帛诚医疗器械科技有限公司 | Potassium ion quantitative detection dry tablet for in vitro diagnosis and preparation method thereof |
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