WO2019047297A1 - 丁香酚/丁香油对软组织上致病菌的杀灭应用 - Google Patents

丁香酚/丁香油对软组织上致病菌的杀灭应用 Download PDF

Info

Publication number
WO2019047297A1
WO2019047297A1 PCT/CN2017/104683 CN2017104683W WO2019047297A1 WO 2019047297 A1 WO2019047297 A1 WO 2019047297A1 CN 2017104683 W CN2017104683 W CN 2017104683W WO 2019047297 A1 WO2019047297 A1 WO 2019047297A1
Authority
WO
WIPO (PCT)
Prior art keywords
eugenol
clove oil
pathogenic bacteria
mbc
staphylococcus aureus
Prior art date
Application number
PCT/CN2017/104683
Other languages
English (en)
French (fr)
Inventor
杜卓
谭淑仪
刘芳
张丽华
林树辉
Original Assignee
佛山科学技术学院
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 佛山科学技术学院 filed Critical 佛山科学技术学院
Publication of WO2019047297A1 publication Critical patent/WO2019047297A1/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/075—Ethers or acetals
    • A61K31/085—Ethers or acetals having an ether linkage to aromatic ring nuclear carbon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the invention relates to the application of eugenol/clove oil, in particular to the killing application of eugenol/eugenol to pathogenic bacteria on soft tissues.
  • Soft tissue refers to the skin, subcutaneous tissue, muscles, tendons, ligaments, joint capsules, synovial sacs, nerves, blood vessels, etc. of the human body. Soft tissue is a more important body tissue.
  • 2-Methoxy-4-(2-propenyl)phenol also known as eugenol, has a molecular formula of C 10 H 12 O 2 , is a colorless or pale yellow liquid, has a strong clove aroma, and is insoluble in water. It is naturally found in many essential oils, especially in clove oil, and eugenol accounts for 84% to 95% of clove oil. At present, research on 2-methoxy-4-(2-propenyl)phenol has been intensively and has been applied to various fields.
  • 2-methoxy-4-(2-propenyl) phenol can also be used as a chemical intermediate to synthesize other chemical products; promote transdermal absorption, 2-methoxy-4-(2-propenyl) Phenol extract promotes transdermal absorption of 5-fluorourea; it has a mosquito-repellent effect and can effectively repel mosquitoes for up to 6 hours. It can be developed as a natural plant mosquito.
  • the invention discloses the killing application of eugenol/clove oil to pathogenic bacteria on soft tissues, including Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus aureus, Staphylococcus aureus, Streptococcus aureus, B Streptococcus, Candida albicans, Escherichia coli.
  • the minimum bactericidal concentration MBC of each strain is determined by a multiple dilution method.
  • the liquid medium test tube showed no turbidity to the naked eye and the number of colonies of the blood agar plate for subculture was less than 5 colonies, and the lowest concentration of eugenol/clove oil was the minimum bactericidal concentration MBC of the pathogenic bacteria.
  • the MBC value of eugenol against Staphylococcus aureus is 0.123ug/ml
  • the MBC value of Staphylococcus aureus is 3.945ug/ml
  • the MBC value of Staphylococcus aureus is 1.973ug/ml, against Streptococcus aureus.
  • the MBC value is 15.78 ug/ml
  • the MBC value for Streptococcus B is 7.891 ug/ml
  • the MBC value for Pseudomonas aeruginosa is 31.562 ug/ml
  • the MBC value for Candida albicans is 0.123 ug/ml.
  • the MBC value of Escherichia coli is 7.891 ug/ml; the MBC value of syring oil to Staphylococcus aureus is 0.014 ug/ml, the MBC value for Staphylococcus aureus is 0.906 ug/ml, and the MBC value for Staphylococcus aureus is 14.500.
  • the MBC value for Streptococcus A is 3.625ug/ml
  • the MBC value for Streptococcus mutans is 0.453ug/ml
  • the MBC value for Pseudomonas aeruginosa is 1.813ug/ml, against Candida albicans
  • the MBC value was 0.014 ug/ml
  • the MBC value for E. coli was 0.057 ug/ml.
  • the present invention is based on Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus aureus, Staphylococcus aureus, Streptococcus aureus, Streptococcus mutans, Candida albicans and Escherichia coli, and investigates the presence of eugenol/clove oil in soft tissues.
  • the minimum bactericidal concentration of pathogenic bacteria investigate its Application value in soft tissue sterilization. Conducive to the expansion of the application range of eugenol / clove oil.
  • Example 1 Determination of the minimum bactericidal concentration (MBC) of eugenol on pathogenic bacteria on soft tissues
  • Reagent ethanol.
  • inoculum Preparation of inoculum: The same type of colonies were inoculated into the broth medium on the purely tested bacteria plate; the streptococci were inoculated into the blood broth medium, cultured at 35 degrees overnight, and the bacterial solution was adjusted to make it It is equivalent to 0.5 McPherson turbidity standard, and then diluted with 1:200 in broth culture medium for use. The diluted bacterial solution was inoculated within 15 min.
  • Species Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus aureus, Staphylococcus aureus, Streptococcus aureus, Streptococcus mutans, Candida albicans, Escherichia coli.
  • Instruments scale pipette, pipette, incubator, pressure cooker.
  • alcohol lamp nozzle
  • test tube test tube rack
  • inoculating ring inoculating ring
  • the tested suspensions with the corrected concentrations were sequentially added to each of the drug-containing tubes, 0.05 ml per tube, and mixed.
  • the tubes were incubated at 35 ° C for 18 h and the results were observed.
  • test tube was inoculated with a volume of 0.01 ml, and the tube with no turbidity and the lowest concentration of eugenol was observed.
  • the tube was transferred to a blood agar plate for subculture, and the number of colonies on the blood agar plate was observed after being cultured at 37 degrees for 24 hours.
  • the number of colonies in the subculture of blood agar plates was 5 colonies, and the lowest concentration of eugenol contained was the lowest bactericidal concentration (MBC) of the bacteria. That is, the lowest drug concentration can kill 99.9% of the original bacteria, see Table 1:
  • Example 2 Determination of the minimum bactericidal concentration (MBC) of clove oil on pathogenic bacteria on soft tissues
  • Reagent ethanol.
  • inoculum Preparation of inoculum: The same type of colonies were inoculated into the broth medium on the purely tested bacteria plate; the streptococci were inoculated into the blood broth medium, cultured at 35 degrees overnight, and the bacterial solution was adjusted to make it It is equivalent to 0.5 McPherson turbidity standard, and then diluted with 1:200 in broth culture medium for use. The diluted bacterial solution was inoculated within 15 min.
  • Species Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus aureus, Staphylococcus aureus, Streptococcus aureus, Streptococcus mutans, Candida albicans, Escherichia coli.
  • Instruments scale pipette, pipette, incubator, pressure cooker.
  • alcohol lamp nozzle
  • test tube test tube rack
  • inoculating ring inoculating ring
  • the tested suspensions with the corrected concentrations were sequentially added to each of the drug-containing tubes, 0.05 ml per tube, and mixed.
  • the tubes were incubated at 35 ° C for 18 h and the results were observed.
  • test tube was inoculated with a volume of 0.01 ml, and the tube with no turbidity and the lowest concentration of clove oil was observed.
  • the tube was transferred to a blood agar plate for subculture, and the number of colonies on the blood agar plate was observed after being cultured at 37 degrees for 24 hours.
  • the number of colonies in the subculture of blood agar plates was 5 colonies, and the lowest concentration of clove oil contained was the lowest bactericidal concentration (MBC) of the bacteria. That is, the lowest drug concentration can kill 99.9% of the original bacteria, see Table 2:

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

本发明公开了丁香酚/丁香油对软组织上致病菌的杀灭应用，所述致病菌包括绿脓杆菌、金黄色葡萄球菌、白色葡萄球菌、柠檬色葡萄球菌、甲型链球菌、乙型链球菌、白色念珠菌、大肠杆菌。考察丁香酚/丁香油对软组织中存在致病菌的最低杀菌浓度，考察其在软组织杀菌消毒方面的应用价值。

Description

丁香酚/丁香油对软组织上致病菌的杀灭应用 技术领域
本发明涉及丁香酚/丁香油的应用，具体为丁香酚/丁香酚对软组织上致病菌的杀灭应用。
背景技术
软组织是指人体的皮肤、皮下组织、肌肉、肌腱、韧带、关节囊、滑膜囊，神经、血管等。软组织是比较重要的一个人体组织。
2-甲氧基-4-(2-丙烯基)苯酚又名丁香酚，分子式为C10H12O2，是无色或苍黄色液体，有强烈的丁香香气，不溶于水。天然存在于多种精油中，尤以丁香油中含量最多，丁香酚占丁香油的84％～95％。目前对2-甲氧基-4-(2-丙烯基)苯酚的研究已经比较深入，并且已应用于各个领域。
抗氧化作用：2-甲氧基-4-(2-丙烯基)苯酚与其它抗氧化剂相比，其还原能力、对H2O2的清除能力、减少脂质过氧化能力的效果非常明显，利用2-甲氧基-4-(2-丙烯基)苯酚可以开发出新型的绿色的抗氧化剂。
其它应用：2-甲氧基-4-(2-丙烯基)苯酚还可作为化工中间体合成其它的化学产品；促进透皮吸收作用，2-甲氧基-4-(2-丙烯基)苯酚提取物促进5-氟脲啶透皮吸收；有祛蚊作用，有效驱避蚊虫时间可达到6h，可开发为一种天然植物祛蚊剂。
发明内容
本发明公开了丁香酚/丁香油对软组织上致病菌的杀灭应用，所述致病菌包括绿脓杆菌、金黄色葡萄球菌、白色葡萄球菌、柠檬色葡萄球菌、甲型链球菌、乙型链球菌、白色念珠菌、大肠杆菌。
本发明采用倍数稀释法对各菌种进行最低杀菌浓度MBC的测定。液体培养基试管无肉眼可见浑浊并且血琼脂平板作次代培养的菌落数量少于5个菌落，其丁香酚/丁香油的最低浓度即为该致病菌的最低杀菌浓度MBC。
其中，丁香酚对金黄色葡萄球菌的MBC值为0.123ug/ml，对白色葡萄球菌的MBC值为3.945ug/ml，对柠檬色葡萄球菌的MBC值为1.973ug/ml，对甲型链球菌的MBC值为15.781ug/ml，对乙型链球菌的MBC值为7.891ug/ml，对绿脓杆菌的MBC值为31.562ug/ml，对白色念珠菌的MBC值为0.123ug/ml，对大肠杆菌的MBC值为7.891ug/ml；丁香油对金黄色葡萄球菌的MBC值为0.014ug/ml，对白色葡萄球菌的MBC值为0.906ug/ml，对柠檬色葡萄球菌的MBC值为14.500ug/ml，对甲型链球菌的MBC值为3.625ug/ml，对乙型链球菌的MBC值为0.453ug/ml，对绿脓杆菌的MBC值为1.813ug/ml，对白色念珠菌的MBC值为0.014ug/ml，对大肠杆菌的MBC值为0.057ug/ml。
本发明以绿脓杆菌、金黄色葡萄球菌、白色葡萄球菌、柠檬色葡萄球菌、甲型链球菌、乙型链球菌、白色念珠菌、大肠杆菌为对象，考察丁香酚/丁香油对软组织中存在致病菌的最低杀菌浓度，考察其 在软组织杀菌消毒方面的应用价值。有利于扩大丁香酚/丁香油的应用范围。
具体实施方式
本领域技术人员将会理解，下列实施例仅用于说明本发明，而不应视为限定本发明的范围。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。
实施例1：丁香酚对软组织上致病菌的最低杀菌浓度(MBC)测定
一、实验材料
药物：丁香酚。
试剂：乙醇。
培养基：普通肉汤、血肉汤、血平板培养基。
接种菌液的准备：在已分纯的待测菌平板上取形态相同的菌落接种于肉汤培养基内；链球菌接种于血肉汤培养基内，35度培养过夜，校正菌液，使其相当于0.5麦氏比浊标准，再用肉汤培养基按1：200稀释后备用。稀释后的菌液在15min内接种。
菌种：绿脓杆菌、金黄色葡萄球菌、白色葡萄球菌、柠檬色葡萄球菌、甲型链球菌、乙型链球菌、白色念珠菌、大肠杆菌。
仪器：刻度吸管、移液枪、恒温箱、高压锅。
其它：酒精灯、吸嘴、试管、试管架、接种环。
二：实验步骤
排列试管15支，除第一支外，每管加入普通肉汤培养基1ml。
先吸取丁香酚原液5.12ml加入液体培养基4.88ml中，混合，吸出分别加入第一管和第二管中，每管1ml，第二管经混合后吸出1ml，加入第三管中，依此类推至第十五管后，吸出1ml弃去，这样各管抗菌药物的最终含量依次为(512、256、128、64、32、16、8、4、2、1、0.5、0.25、0.13、0.06、0.03ug/ml)。另设培养液对照，检测菌生长对照和质控菌生长对照各一支。
将已校正浓度的待测悬液依次加入各含药管内，每管0.05ml，混匀。各试管置35℃培养18h后观察结果。
再以0.01ml容量接种环取肉眼观察无浑浊且丁香酚最低浓度的试管，移种一环于血琼脂平板作次代培养，经37度培养24h后观察血琼脂平板的菌落数量。
三：实验结果
血琼脂平板作次代培养的菌落数量少5个菌落，其所含丁香酚最低浓度作为该菌的最低杀菌浓度(MBC)。即最低药物浓度能杀死99.9％原始种入的细菌，见表一：
表一丁香酚对软组织上致病菌的最低杀菌浓度MBC(浓度单位ug/ml)
Figure PCTCN2017104683-appb-000001
Figure PCTCN2017104683-appb-000002
所有结果均显示丁香酚对软组织致病菌的最低杀菌浓度MBC在32ug/ml以下。
实施例2：丁香油对软组织上致病菌的最低杀菌浓度(MBC)测定
一、实验材料
药物：丁香油。
试剂：乙醇。
培养基：普通肉汤、血肉汤、血平板培养基。
接种菌液的准备：在已分纯的待测菌平板上取形态相同的菌落接种于肉汤培养基内；链球菌接种于血肉汤培养基内，35度培养过夜，校正菌液，使其相当于0.5麦氏比浊标准，再用肉汤培养基按1：200稀释后备用。稀释后的菌液在15min内接种。
菌种：绿脓杆菌、金黄色葡萄球菌、白色葡萄球菌、柠檬色葡萄球菌、甲型链球菌、乙型链球菌、白色念珠菌、大肠杆菌。
仪器：刻度吸管、移液枪、恒温箱、高压锅。
其它：酒精灯、吸嘴、试管、试管架、接种环。
二：实验步骤
排列试管15支，除第一支外，每管加入普通肉汤培养基1ml。
先吸取丁香油原液5.12ml加入液体培养基4.88ml中，混合，吸出分别加入第一管和第二管中，每管1ml，第二管经混合后吸出1ml， 加入第三管中，依此类推至第十五管后，吸出1ml弃去，这样各管抗菌药物的最终含量依次为(116、58、29、14.5、7.25、3.625、1.813、0.906、0.453、0.227、0.113、0.057、0.028、0.014、0.007ug/ml)。另设培养液对照，检测菌生长对照和质控菌生长对照各一支。
将已校正浓度的待测悬液依次加入各含药管内，每管0.05ml，混匀。各试管置35℃培养18h后观察结果。
再以0.01ml容量接种环取肉眼观察无浑浊且丁香油最低浓度的试管，移种一环于血琼脂平板作次代培养，经37度培养24h后观察血琼脂平板的菌落数量。
三：实验结果
血琼脂平板作次代培养的菌落数量少5个菌落，其所含丁香油最低浓度作为该菌的最低杀菌浓度(MBC)。即最低药物浓度能杀死99.9％原始种入的细菌，见表二：
表二丁香油对软组织致病菌的最低杀菌浓度MBC(浓度单位ug/ml)
Figure PCTCN2017104683-appb-000003
所有结果均显示丁香油对软组织上致病菌的最低杀菌浓度MBC 在14.50ug/ml以下。
以上对本发明的较佳实施方式进行了具体说明，但本发明创造并不限于所述实施例，熟悉本领域的技术人员在不违背本发明精神的前提下还可作出种种的等同变型或替换，这些等同的变型或替换均包含在本申请权利要求所限定的范围内。

Claims (10)

  1. 丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，所述致病菌包括绿脓杆菌、金黄色葡萄球菌、白色葡萄球菌、柠檬色葡萄球菌、甲型链球菌、乙型链球菌、白色念珠菌、大肠杆菌。
  2. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，采用倍数稀释法对各致病菌进行最低杀菌浓度MBC的测定。
  3. 根据权利要求2所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，液体培养基试管无肉眼可见浑浊并且血琼脂平板作次代培养的菌落数量少于5个菌落，其丁香酚/丁香油的最低浓度即为该致病菌的最低杀菌浓度MBC。
  4. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对金黄色葡萄球菌的MBC值为0.123ug/ml，丁香油对金黄色葡萄球菌的MBC值为0.014ug/ml。
  5. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对白色葡萄球菌的MBC值为3.945ug/ml，丁香油对白色葡萄球菌的MBC值为0.906ug/ml。
  6. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对柠檬色葡萄球菌的MBC值为1.973ug/ml，丁香油对柠檬色葡萄球菌的MBC值为14.500ug/ml。
  7. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对甲型链球菌的MBC值为15.781g/ml， 丁香油对甲型链球菌的MBC值为3.625ug/ml。
  8. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对乙型链球菌的MBC值为7.891ug/ml，丁香油对乙型链球菌的MBC值为0.453ug/ml。
  9. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对绿脓杆菌的MBC值为31.562ug/ml，丁香油对绿脓杆菌的MBC值为1.813ug/ml。
  10. 根据权利要求1所述的丁香酚/丁香油对软组织上致病菌的杀灭应用，其特征在于，丁香酚对白色念珠菌的MBC值为0.123ug/ml，丁香油对白色念珠菌的MBC值为0.014ug/ml。
PCT/CN2017/104683 2017-09-08 2017-09-30 丁香酚/丁香油对软组织上致病菌的杀灭应用 WO2019047297A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710807691.XA CN107519247A (zh) 2017-09-08 2017-09-08 丁香酚/丁香油对软组织上致病菌的杀灭应用
CN201710807691.X 2017-09-08

Publications (1)

Publication Number Publication Date
WO2019047297A1 true WO2019047297A1 (zh) 2019-03-14

Family

ID=60736553

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2017/104683 WO2019047297A1 (zh) 2017-09-08 2017-09-30 丁香酚/丁香油对软组织上致病菌的杀灭应用

Country Status (2)

Country Link
CN (1) CN107519247A (zh)
WO (1) WO2019047297A1 (zh)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102796616A (zh) * 2011-05-26 2012-11-28 王凌 一种可抑菌的百草香天然植物香精
CN104824120A (zh) * 2015-05-11 2015-08-12 江苏天晟药业有限公司 丁香酚在食源性致病菌的体外抑制中的应用
CN105705014A (zh) * 2013-03-15 2016-06-22 普渡研究基金会 抗微生物组合物和使用方法
CN107019138A (zh) * 2017-06-15 2017-08-08 佛山科学技术学院 一种丁香酚组合液及制备方法和应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102796616A (zh) * 2011-05-26 2012-11-28 王凌 一种可抑菌的百草香天然植物香精
CN105705014A (zh) * 2013-03-15 2016-06-22 普渡研究基金会 抗微生物组合物和使用方法
CN104824120A (zh) * 2015-05-11 2015-08-12 江苏天晟药业有限公司 丁香酚在食源性致病菌的体外抑制中的应用
CN107019138A (zh) * 2017-06-15 2017-08-08 佛山科学技术学院 一种丁香酚组合液及制备方法和应用

Also Published As

Publication number Publication date
CN107519247A (zh) 2017-12-29

Similar Documents

Publication Publication Date Title
Sebiomo et al. Comparative studies of antibacterial effect of some antibiotics and ginger (Zingiber officinale) on two pathogenic bacteria
CN109988225B (zh) 具抗菌活性的多肽及其应用
KR20200039847A (ko) 하이포아염소산 수용액
WO2019144830A1 (zh) 一种宽裂解谱绿脓杆菌噬菌体及其消毒应用
KLEINFELD et al. Effects of topical anesthetics on growth of microorganisms
CN110151752A (zh) 一种茶多酚类组合物及其在制备抗猪链球菌药物中的应用
CN110123806A (zh) 表没食子儿茶素没食子酸酯在制备抗猪链球菌药物中的应用
WO2019028937A1 (zh) 丁香酚/丁香油在抑制口腔条件致病菌的应用
Kleinfeld et al. Inhibition of microorganisms by topical anesthetics
Ekhaise et al. Antibacterial properties and preliminary phytochemical analysis of methanolic extract of mistletoe (Tapinanthus bangwensis
Smith et al. A new protocol for antimicrobial testing of oils
CN110227071B (zh) 复方龙脑抗菌剂
Andrews et al. Antimicrobial hydrogels formed by crosslinking polyallylamine with aldaric acid derivatives
Morton et al. The bacteriostatic and bactericidal actions of some mercurial compounds on hemolytic streptococci: in vivo and in vitro studies
WO2019047297A1 (zh) 丁香酚/丁香油对软组织上致病菌的杀灭应用
CN110898075A (zh) 聚六亚甲基双胍在抑制、杀灭红色毛癣菌、须癣毛癣菌、犬小孢子菌及白念珠菌中的应用
CN109924201A (zh) 一种苝醌类化合物的复配杀菌剂
Datsugwai et al. Phytochemical analysis and antimicrobial activity of baobab (Adansonia digitata) leaves and stem bark extracts on Staphylococcus aureus and Escherichia coli
Korji Inhibition of nitrate reductase production from gramnegative bacteria using Ziziphus spina-christi extract and comparing with some antibiotics
WO2017038872A1 (ja) 抗真菌活性を有する組成物
Airaodion et al. Investigation of antibacterial activity of Vernonia amygdalina leaf extracts against gram-positive and gram-negative bacteria
Aiemsaard et al. Antibacterial efficacy of essential oil spray formulation for post-milking disinfection in dairy cows
She et al. Repurposing sitafloxacin, prulifloxacin, tosufloxacin, and sisomicin as antimicrobials against biofilm and persister Cells of Pseudomonas aeruginosa
CN109362796A (zh) 艾草抗菌剂及其制备方法
Hawassa et al. Anti-Bacterial effect of garlic (Allium sativum) against clinical isolates of Staphylococcus aureus and Escherichia coli from patients attending hawassa referral hospital, Ethiopia

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17924210

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17924210

Country of ref document: EP

Kind code of ref document: A1