WO2019041270A1 - 样本检测装置、样本分析仪及样本检测方法 - Google Patents

样本检测装置、样本分析仪及样本检测方法 Download PDF

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Publication number
WO2019041270A1
WO2019041270A1 PCT/CN2017/100046 CN2017100046W WO2019041270A1 WO 2019041270 A1 WO2019041270 A1 WO 2019041270A1 CN 2017100046 W CN2017100046 W CN 2017100046W WO 2019041270 A1 WO2019041270 A1 WO 2019041270A1
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WIPO (PCT)
Prior art keywords
liquid
access point
tested
sample
pipeline
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PCT/CN2017/100046
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English (en)
French (fr)
Inventor
刘隐明
滕锦
石汇林
Original Assignee
深圳迈瑞生物医疗电子股份有限公司
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Application filed by 深圳迈瑞生物医疗电子股份有限公司 filed Critical 深圳迈瑞生物医疗电子股份有限公司
Priority to PCT/CN2017/100046 priority Critical patent/WO2019041270A1/zh
Priority to CN201780094071.4A priority patent/CN111033254A/zh
Publication of WO2019041270A1 publication Critical patent/WO2019041270A1/zh
Priority to US16/801,069 priority patent/US11383232B2/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

Definitions

  • the present invention relates to the field of medical device technology, and in particular, to a sample detecting device, a sample analyzer, and a sample detecting method.
  • the core detection component in the five-class blood cell analyzer is a flow chamber.
  • the detecting unit detects the volume of the blood cells and the specific characteristics of the contents by a light scattering method, and gives a cell counting result and various classification results based on these specific characteristics. Due to the development of the reagent system, blood cells can be processed by different reagents to obtain more specific characteristic signals to distinguish more abnormal information and provide valuable test results for the clinic.
  • the reaction system and the detection system are separated into two parts, and each reagent reaction is completed in a separate reaction cell.
  • the reactants completed in the reaction are transported to the flow chamber for detection, so that there is a risk of direct cross-contamination of the reactants.
  • the diluent is generally used for rinsing, and since the adhesion of the individual reagents is relatively strong, the general dilution rinsing cannot completely rinse the residue, and the next sample is taken. When these residues are encountered during transportation, the purity of the sample will be affected and the measurement results will be affected.
  • Embodiments of the present invention provide a sample detecting device, a sample analyzer, and a sample detecting method.
  • a pipeline assembly including a first conduit and a second conduit;
  • the detection assembly including a sample needle, the sample needle including a first access point;
  • reaction assembly comprising a first reaction cell group and a second reaction cell group, the first reaction cell group being connected to a second access point of the first conduit, the second reaction cell group Connecting to the second conduit, the reaction assembly is for processing a biological sample to form a liquid to be tested;
  • the liquid to be tested in the first reaction pool group flows to the first access point of the sample needle through the second access point of the first pipeline, and the liquid to be tested in the second reaction pool group Flowing through the second pipeline to the office
  • the first access point of the sample needle is such that the liquid to be tested in the first reaction cell group or the second reaction cell group flows into the sample needle of the detection component for detection.
  • the embodiment of the present invention further provides a sample detecting method, where the sample detecting device performs the detecting, and the sample detecting method includes:
  • the first biological sample and the second biological sample are respectively placed in the first reaction pool group and the second reaction pool group to be processed to form a liquid to be tested;
  • the invention also provides a sample analyzer comprising a sampling component and a sample detecting device, the sampling component comprising a sampler, the sampler collecting a biological sample and moving to above the reaction component to A sample is dispensed into the reaction assembly.
  • the sample detecting device, the sample analyzer and the sample detecting method provided by the embodiments of the present invention connect the first reaction pool group and the second reaction pool group to the first pipeline and the second pipeline, respectively, so that the first reaction pool group
  • the liquid to be tested and the liquid to be tested in the second reaction cell group can be pushed into the detection component from different pipelines, and there is no interference between them, which is beneficial to ensure the accuracy of the detection result of the detection component.
  • FIG. 1 is a schematic diagram of a sample detecting device according to Embodiment 1 of the present invention.
  • FIG. 2 to 7 are schematic views of different communication structures of the second pipe and the second reaction cell group of FIG. 1;
  • FIG. 8 to 10 are schematic views of different communication structures of the second conduit of FIG. 1 and the first access point;
  • FIG. 11 is a schematic diagram of a sample detecting device according to Embodiment 2 of the present invention.
  • FIG. 12 is a schematic diagram of a sample detecting apparatus according to Embodiment 3 of the present invention.
  • FIG. 13 is a flowchart of a sample detection method according to an embodiment of the present invention.
  • FIG. 15 is a flowchart of still another sample detecting method according to an embodiment of the present invention.
  • Figure 16 is a schematic illustration of a sample analyzer of the present invention.
  • a first embodiment of the present invention provides a sample detecting device 100 including a pipeline assembly 11, a detecting assembly 12, and a reaction assembly 13.
  • the tubing assembly 11 is connected between the detecting assembly 12 and the reaction assembly 13, and the tubing assembly 11 is used for the test liquid in the reaction assembly 13 to flow into the detecting assembly 12 for detection.
  • the pipeline assembly 11 includes a first conduit 111 and a second conduit 112.
  • the detection assembly 12 includes a sample needle 121 that includes a first access point S1.
  • the reaction assembly 13 is for processing a biological sample to form a test solution.
  • the reaction assembly 13 includes a first reaction cell group 131 for forming a first liquid to be tested and a second reaction cell group 132 for forming a second liquid to be tested, and the first reaction cell group 131 is connected to the first conduit 111.
  • the second reaction pool group 132 is connected to the second pipeline 112; the liquid to be tested in the first reaction pool group 131 passes through the second access point S2 of the first pipeline 111.
  • the liquid to be tested in the second reaction cell group 132 flows to the first access point S1 of the sample needle 121 via the second conduit 112 So that the liquid to be tested in the first reaction cell group 131 or the second reaction cell group 132 flows into the sample needle 121 of the detection component 12 for detection.
  • the first liquid to be tested and the second liquid to be tested may be sequentially entered into the detecting component 12 according to a sequence of formation to detect the detection speed of the sample detecting device 100.
  • the first liquid to be tested and the second liquid to be tested may also enter the detecting component 12 for detection according to other preset sequences.
  • the first reaction cell group 131 is configured to form a first test solution for detecting a white blood cell count or a white blood cell classification
  • the second reaction pool group 132 is configured to form a second test liquid for detecting a reticulocyte count.
  • the first reaction cell group 131 and the second reaction cell group 132 are respectively in communication with the first pipe 111 and the second pipe 112, so that even the first liquid to be tested and/or the second in the first reaction cell group 131
  • the second liquid to be tested in the reaction cell group 132 is relatively easy to adhere to the pipe wall, and in the case that it is difficult to clean, since the first liquid to be tested and the second liquid to be tested can pass through different pipelines (corresponding to the first pipeline 111 respectively)
  • the second pipe 112) flows into the sample needle 121 of the detecting component 12 to detect, does not interfere with each other, and is advantageous for ensuring the accuracy of the detection result of the detecting component 12.
  • the first reaction pool group 131 includes a first reaction cell 1311, a first branch 1312, and a first switching member 1313.
  • the first reaction pool 1311 is configured to form a first test unit.
  • the first branch 1312 is connected between the first reaction tank 1311 and the second access point S2, and the first switching member 1313 is disposed on the first branch 1312.
  • the first switching member 1313 is used to implement the cutting and communication of the first branch 1312.
  • the first switching member 1313 can be a valve.
  • the flow of the first liquid to be tested is specifically: the first liquid to be tested formed in the first reaction tank 1311 flows into the first branch 1312, and if the first switching member 1313 is opened, the first switching member 1313 realizes the first branch 1312.
  • the first test liquid in the first branch 1312 flows into the second access point S2, and flows into the first line 111 through the second access point S2; if the first switching member 1313 is closed, the first switch When the first 1312 is cut off, the first liquid to be tested in the first branch 1312 cannot flow into the second access point S2, and the first liquid to be tested cannot enter the first pipeline 111.
  • the second reaction cell group 132 includes a second reaction cell 1321 , a second branch 1322 , and a second switching member 1323 .
  • the second reaction cell 1321 is configured to form a second to be tested.
  • the second branch 1322 has one end connected to the second reaction tank 1321, and the second branch 1322 is connected to the second line 112, and the second switching member 1323 is disposed at the second branch 1322.
  • the second switching member 1323 is configured to implement cutting and communication of the second branch 1322.
  • the second switching member 1323 can be a valve.
  • the communication or disconnection of the second branch 1322 is achieved by the opening or closing of the second switching member 1323.
  • the communication structure between the second reaction pool group 132 and the second pipeline 112 is as follows:
  • the second conduit 112 is connected to the second reaction cell group 132 to connect the second reaction cell group 132 to the second conduit 112.
  • one end of the second branch 1322 is connected to the second reaction pool 1321, and the other end of the second branch 1322 is directly connected to the third end G3 of the second conduit 112.
  • the flow of the liquid to be tested in the second reaction cell group 132 is specifically: the second liquid to be tested formed in the second reaction cell 1321 flows into the second The branch 1322, if the second switching member 1323 is opened, that is, the second switching member 1323 realizes the communication of the second branch 1322, the second liquid to be tested in the second branch 1322 flows into the third end of the second conduit 112.
  • the portion G3 further flows into the second conduit 112; if the second switching member 1323 is closed, that is, when the second switching member 1323 realizes the cutting of the second branch 1322, the second liquid to be tested in the second branch 1322 cannot flow into the second portion Two lines 112.
  • the communication structure of the second pipeline 112 and the second reaction pool group 132 enables the liquid to be tested in the second reaction pool group 132 to directly flow into the second pipeline 112, and does not flow into the first pipeline 111 with the first reaction pool group 131.
  • the liquid to be tested crosses, and the connecting line of the second pipeline 112 directly connected to the second reaction pool group 132 is relatively simple and the cost is low.
  • the second reaction pool group 132 is connected to the ninth access point S9 of the second pipeline 112, and the second reaction pool group 132 is to be tested.
  • the liquid flows into the second line 112 from the ninth access point S9 to connect the second reaction pool group 132 to the second line 112.
  • one end of the second branch 1322 is connected to the second reaction pool 1321, and the other end of the second branch 1322 is connected to the ninth access point S9 of the second conduit 112.
  • the flow of the liquid to be tested in the second reaction cell group 132 is specifically: the second liquid to be tested formed in the second reaction cell 1321 flows into the second The branch 1322, if the second switching member 1323 is opened, that is, the second switching member 1323 realizes the communication of the second branch 1322, the second liquid to be tested in the second branch 1322 flows into the ninth connection of the second conduit 112.
  • the entry point S9 further flows into the second conduit 112; if the second switching member 1323 is closed, that is, the second switching member 1323 realizes the cutting of the second branch 1322, the second liquid to be tested in the second branch 1322 cannot flow into the second branch 1322.
  • the communication structure of the second pipeline 112 and the second reaction pool group 132 enables the liquid to be tested in the second reaction pool group 132 to directly flow into the second pipeline 112, and does not flow into the first pipeline 111 with the first reaction pool group 131.
  • the liquid to be tested crosses, and the connecting structure of the second reaction tank group 132 directly connected to the second pipeline 112 is relatively simple and the cost is low.
  • the second reaction pool group 132 is connected to the fifth access point S5 of the first pipeline 111, and one end of the second pipeline 112 is connected to the first pipeline.
  • the eighth access point S8 of 111 Specifically, one end of the second branch 1322 is connected to the second reaction pool 1321, and the other end of the second branch 1322 is first connected to the fifth access point S5 of the first pipeline 111, and the second tube
  • the third end of the path 112 is re-accessed to the eighth access point S8 of the first conduit 111.
  • the fifth access point S5 and the eighth access point S8 are further away from the first access point S1 than the second access point S2, so that the liquid to be tested in the first reaction pool group 131 is in the second During the process of the access point S2 flowing to the first access point S1, the liquid to be tested in the first reaction pool group 131 does not pass through the fifth access point S5 and the eighth access point S8, so in the second reaction pool
  • the liquid to be tested of the group 132 flows through the fifth access point S5 to the eighth access point S8 until the second pipeline 112
  • the liquid to be tested of the second reaction pool group 132 is also not combined with the first reaction pool group.
  • the liquids to be tested in 131 cross each other.
  • the eighth access point S8 is located at the right side of the fifth access point S5.
  • the eighth access point is located on the left side of the fifth access point S5.
  • the flow of the liquid to be tested in the second reaction cell group 132 is specifically: the second liquid to be tested formed in the second reaction cell 1321 flows into the second The branch circuit 1322, if the second switching member 1323 is opened, that is, the second switching member 1323 realizes the communication of the second branch 1322, the second liquid to be tested in the second branch 1322 flows into the fifth access point S5, and The fifth access point S5 flows to the eighth access point S8 to enter the second conduit 112; if the second switching member 1323 is closed, that is, the second switching member 1323 realizes the cutting of the second branch 1322, the second branch The second liquid to be tested in the road 1322 cannot flow into the fifth access point S5.
  • the second reaction pool group 132 is connected to the fifth access point S5 of the first pipeline 111 , and one end of the second pipeline 112 is connected to the first pipeline 111 .
  • the fifth access point S5 and the eighth access point S8 are closer to the first access point S1 than the second access point S2.
  • the flow of the liquid to be tested in the second reaction cell group 132 is specifically: the second liquid to be tested formed in the second reaction cell 1321 flows into the second The branch circuit 1322, if the second switching member 1323 is opened, that is, the second switching member 1323 realizes the communication of the second branch 1322, the second liquid to be tested in the second branch 1322 flows into the fifth access point S5, and Fifth connection
  • the entry point S5 flows to the eighth access point S8 to enter the second line 112; if the second switching member 1323 is closed, that is, the second switching member 1323 realizes the cutting of the second branch 1322, the second branch 1322 is The second test solution cannot flow into the fifth access point S5.
  • the flow of the liquid to be tested in the first reaction cell group 131 is specifically: the first test liquid formed in the first reaction cell 1311 flows into the first branch 1312, and the first switch 1313 is turned on, the first switch When the device 1313 realizes the communication of the first branch 1312, the first liquid to be tested in the first branch 1312 flows into the second access point S2, and flows from the second access point S2 to the fifth access point S5. And the eighth access point S8 to flow into the first access point S1.
  • the first liquid to be tested and the second liquid to be tested may flow through the fifth access point S5 to the eighth access point S8, but the distance between the fifth access point S5 and the eighth access point S8 is shorter.
  • the cross-contamination that can be brought about can be neglected.
  • the second pipeline 112 and the second reaction pool group 132 are commonly connected to the fifth access point S5 of the first pipeline 111.
  • the second reaction cell group 132 is connected to the second conduit 112. Specifically, one end of the second branch 1322 is connected to the second reaction pool 1321, and the other end of the second branch 1322 and the third end G3 of the second conduit 112 pass through the four-way.
  • the head ie, the fifth access point S5 is simultaneously connected to the first line 111.
  • the flow of the liquid to be tested in the second reaction cell group 132 is specifically: the second liquid to be tested formed in the second reaction cell 1321 flows into the second The branch circuit 1322, if the second switching member 1323 is opened, that is, the second switching member 1323 realizes the communication of the second branch 1322, the second liquid to be tested in the second branch 1322 flows into the fifth access point S5, and The fifth access point S5 flows to the second pipeline 112; if the second switching member 1323 is closed, that is, the second switching member 1323 realizes the cutting of the second branch 1322, the second liquid to be tested in the second branch 1322 It is impossible to flow into the fifth access point S5.
  • the detecting component 12 further includes a flow chamber 122 , a sheath liquid pool 123 , a fifth switching member 124 , a first waste liquid pool 125 , and a sixth switching member 126 .
  • the flow chamber 122 has a liquid inlet 122a, a sheath inlet 122b, and an outlet 122c, the liquid inlet 122a is connected to the first access point S1 of the sample needle 121, and the sheath liquid inlet 122b is connected to the sheath liquid pool 123.
  • the sheath liquid pool 123 is for storing the sheath liquid
  • the fifth switching member 124 is connected between the sheath liquid pool 123 and the sheath liquid inlet 122b, and the fifth switching member 124 is for cutting off the channel
  • the outlet 122c is connected to the first waste liquid pool 125
  • the sixth switching member 126 is connected to the Between the first waste liquid pool 125 and the outlet 122c, the sixth switching member 126 is used to realize the cutting and communication of the channel in which it is located.
  • the fifth switching member 124 can be a valve.
  • the sixth switching member 126 can be a valve.
  • the sheath liquid pool 123 is connected to the third gas storage tank, and when the two are connected, the second positive pressure provided by the third gas storage tank pushes the sheath liquid into the flow chamber 122.
  • a detection sensor is disposed in the flow chamber 122, and the detection sensor is configured to detect the flow of the liquid to be tested.
  • the first waste liquid pool 125 is connected to the atmosphere or a negative pressure.
  • the liquid to be tested (the first liquid to be tested or the second liquid to be tested) enters the flow chamber 122 from the liquid inlet 122 a to be tested, and is connected when the fifth switching member 124 is opened.
  • the sheath liquid pool 123 and the sheath liquid inlet 122b the sheath liquid in the sheath liquid pool 123 flows into the flow chamber 122 through the sheath liquid inlet 122b, and the sheath liquid wraps the liquid to be tested to form a liquid flow to be tested.
  • the sixth switching member 126 is opened, that is, when the first waste liquid pool 125 and the outlet 122c are connected, the detected liquid flow to be tested enters the first waste liquid pool from the outlet 122c. 125.
  • connection between the second conduit 112 and the first access point S1 of the sample needle 121 is as follows:
  • the fourth end G4 of the second conduit 112 is connected to the tenth access point S10 on the first conduit 111, and the tenth access Point S10 is connected to the first access point S1 of the sample needle 121; the liquid to be tested in the second reaction pool group 132 flows to the tenth of the first line 111 through the second line 112
  • the access point S10 flows from the tenth access point S10 of the first conduit 111 to the first access point S1 of the sample needle 121.
  • the specific flow direction of the first test liquid flowing into the detecting component 12 in the first pipeline 111 is: the first liquid to be tested flows from the second access point S2 to the first access point S1, and then the first from the sample needle 121.
  • the access point S1 flows into the liquid to be tested 122a of the flow chamber 122.
  • the specific flow direction of the second liquid to be tested flowing into the detecting component 12 in the second conduit 112 is: the second liquid to be tested flows from the second conduit 112 to the tenth access point S10 of the first conduit 111, and Since the first The tenth access point S10 of the line 111 flows to the first access point S1 of the sample needle 121.
  • the communication structure of the second conduit 112 and the first access point S1 of the sample needle 121 in this embodiment is such that the liquid to be tested in the first conduit 111 and the second conduit 112 is from the first reaction chamber 1311 and the second Each of the reaction cells 1321 flows into the corresponding pipeline independently, and then flows independently to the first access point S1 of the sample needle 121, and the only intersection of the two liquids to be tested is required to pass through the first access point S1.
  • the sample needle 121 can enter the flow chamber 122 for detection. According to the experiments of the inventors, when a flow chamber 122 is entered, a joint is shared, and the cross-contamination is not as serious as expected.
  • the two pipelines are arranged side by side, which can largely avoid cross-contamination and achieve product design. It is required that the sample detecting device 100 in this embodiment can achieve better anti-cross-contamination effect by adding a low-cost configuration in which one pipeline and the other pipeline are separately provided.
  • the fourth end G4 of the second conduit 112 is connected to the tenth access point S10 on the first conduit 111, and the tenth access Point S10 is connected to the first access point S1 of the sample needle 121; the liquid to be tested in the second reaction pool group 132 flows to the tenth of the first line 111 through the second line 112
  • the access point S10 flows from the tenth access point S10 of the first conduit 111 to the first access point S1 of the sample needle 121. It can be understood that when the liquid to be tested in the first reaction pool group 131 flows from the second access point S2 of the first pipeline 111 to the first access point S1 of the sample needle 121, it will flow. Passing through the tenth access point S10 on the first conduit 111.
  • the tenth access point S10 is disposed close to the first access point S1.
  • the specific flow direction of the first test liquid flowing into the detecting component 12 in the first pipeline 111 is: the first liquid to be tested flows from the second access point S2 to the tenth access point S10, and then flows to the first access point. S1, then flows from the first access point S1 of the sample needle 121 into the liquid to be tested 122a of the flow chamber 122.
  • the specific flow direction of the second liquid to be tested flowing into the detecting component 12 in the second conduit 112 is: the second liquid to be tested flows from the second conduit 112 to the tenth access point S10 of the first conduit 111, and The tenth access point S10 from the first conduit 111 flows to the first access point S1 of the sample needle 121.
  • the communication structure of the second conduit 112 and the first access point S1 of the sample needle 121 in this embodiment is such that the liquid to be tested in the first conduit 111 and the second conduit 112 is at the tenth access point S10 and There is a common portion between the access points S1, and the two liquids to be tested share a joint when entering the flow chamber 122.
  • the tenth access point S10 is disposed close to the first access point S1
  • the length between the first access point S1 and the first access point S1 is very short, so even if the liquid to be tested has a common flow portion and shares one connection
  • the cross-contamination of the head and the sample needle 121 is not expected to be serious.
  • the two pipelines are arranged side by side, and cross-contamination can be largely avoided to meet the product design requirements, that is, the sample detecting device 100 in this embodiment passes.
  • Adding a low-cost configuration in which one pipe and the other pipe are separately provided can achieve better cross-contamination.
  • the second conduit 112 is connected to the first access point S1 of the detection component 12. Specifically, the fourth end G4 of the second conduit 112 directly accesses the first access point S1 to reduce the flow path of the second liquid to be tested.
  • sample needle 121, the first conduit 111 and the second conduit 112 are connected by a four-way head (first access point S1).
  • the specific flow direction of the first test liquid flowing into the detecting component 12 in the first pipeline 111 is: the first liquid to be tested flows from the second access point S2 to the first access point S1, and then the first from the sample needle 121.
  • the access point S1 flows into the liquid to be tested 122a of the flow chamber 122.
  • the specific flow direction of the second liquid to be tested flowing into the detecting component 12 in the second conduit 112 is: the second liquid to be tested flows from the second conduit 112 to the first access point S1, and then from the sample needle 121 An access point S1 flows into the liquid to be tested 122a of the flow chamber 122.
  • the communication structure of the second conduit 112 and the first access point S1 of the sample needle 121 in this embodiment is such that the liquid to be tested in the first conduit 111 and the second conduit 112 is from the first reaction chamber 1311 and the second Each of the reaction cells 1321 flows into the corresponding pipeline independently, and then flows independently to the first access point S1 of the sample needle 121, and the only intersection of the two liquids to be tested is required to pass through the first access point S1.
  • the sample needle 121 can enter the flow chamber 122 for detection. According to the experiments of the inventors, when a flow chamber 122 is entered, a joint is shared, and the cross-contamination is not as serious as expected.
  • the two pipelines are arranged side by side, which can largely avoid cross-contamination and achieve product design. It is required that the sample detecting device 100 in this embodiment can achieve better anti-cross-contamination effect by adding a low-cost configuration in which one pipeline and the other pipeline are separately provided.
  • the sample detecting apparatus 100 further includes a sample applying component 14 configured to set the first sample liquid preparation section or the second liquid to be tested in the preparation section by setting the sample assembly 14 The test solution is pushed into the sample needle 121 of the detection assembly 12.
  • the sample insertion component 14 is respectively connected to the third access point S3 of the first pipeline 111 and the fourth access point S4 of the second pipeline 112, where the third access point S3 is located.
  • the liquid to be tested in 132 flows to the fourth access point S4 of the second conduit 112 via the second conduit 112, and flows into the fourth access point S4 through the second conduit 112.
  • the second liquid to be tested is prepared.
  • the sample introduction assembly 14 includes a syringe 141 , a selection switching member 142 disposed at an injection port of the injector 141 , a fourth branch 143 , and a fifth branch 144 .
  • One end of the branch 143 and the fifth branch 144 are respectively connected to the third access point S3 and the fourth access point S4, and the fourth branch 143 and the fifth branch 144 are respectively The other end is commonly provided in the selection switching member 142, and the selection switching member 142 is configured to switch the fourth branch 143 or the fifth branch 144 to communicate with the injector 141.
  • the selection switching member 142 can be a selection valve.
  • the injection liquid in the syringe 141 is pushed from the fourth branch 143 into the third access point S3 to prepare the first waiting liquid in the first test liquid.
  • the measuring liquid is pushed into the first access point S1 for detection by the detecting component 12;
  • the injection liquid in the syringe 141 is pushed from the fifth branch 144 into the fourth access point S4 to prepare the second liquid to be tested in the second waiting portion.
  • the test solution is pushed into the first access point S1 for detection by the detection component 12.
  • the selection switching member 142 is disposed such that when the liquid to be tested of the two pipelines of the sample detecting device 100 needs to flow from the first access point S1 into the flow chamber 122 for detection, the switching member 142 can be selected according to the actual required setting.
  • the branch is turned on so that the corresponding liquid to be tested can be pushed into the flow chamber 122 for detection.
  • the sample detecting device 100 further includes a sampling component 15 that accesses the first conduit 111 and/or the second conduit 112, and the sampling assembly 15 Forming a negative pressure to correspondingly extract the liquid to be tested in the first reaction cell group 131 and/or the liquid to be tested in the second reaction cell group 132 to the first liquid preparation stage and/or Or the second liquid to be tested is prepared in the section.
  • the sampling component 15 includes a second waste liquid pool 151 and a seventh switch.
  • a negative pressure is formed in the second waste liquid pool 151
  • the seventh switching member 152 is connected to the second waste liquid pool 151 and the first pipeline 111 and the second pipeline 112.
  • the seventh switching member 152 is used to implement cutting and communication.
  • the seventh switching member 152 can be a valve.
  • connection manner of the sampling component 15 in the pipeline component 11 is as follows:
  • the first conduit 112 is connected to the first access point S1.
  • the sampling assembly 15 is commonly connected to the first conduit 111 and The second end portion G2 and the fourth end portion G4 of the second conduit 112.
  • the second waste liquid pool 151 is connected to the ends of the first pipeline 111 and the second pipeline 112, a negative pressure is formed in the second waste liquid pool 151, and the seventh switching member 152 is opened to make the second waste liquid pool 151.
  • the first conduit 112 is connected to the first access point S1 .
  • the sampling component 15 is connected to the first conduit 111 .
  • the sampling assembly 15 is connected to the second conduit 112 as an alternative embodiment.
  • the second waste liquid pool 151 is connected to the second pipeline 112, and a negative pressure is formed in the second waste liquid pool 151, and the seventh switching member 152 is opened to connect the second waste liquid pool 151 and the second pipeline 112.
  • the liquid to be tested in the first line 111 and/or the second line 112 is drawn into the first liquid preparation section and/or the second liquid preparation section.
  • sampling component 15 in FIG. 8 to FIG. 10 is correspondingly arranged according to the different communication structure of the second conduit 112 and the first access point S1.
  • the above enumerated manner is only an example, and is not limited to the sampling component 15 Other settings.
  • the sample detecting device 100 further includes a fourth switching member 16, the fourth switching member 16 is disposed on the first pipeline 111, and the fourth switching member 16 is located at the Between the third access point S3 and the second access point S2, the fourth switching component 16 is configured to implement cutting and communication.
  • the fourth switching member 16 can be a valve. Controlling the disconnection and communication between the second access point S2 and the third access point S3 by setting the fourth switching component 16 to control the first waiting in the first reaction pool group 131 Whether the liquid measurement can enter the first liquid preparation section to be tested.
  • Opening the fourth switching member 16 the first liquid to be tested in the first reaction cell group 131 can enter the first liquid preparation portion to be tested; and the fourth switching member 16 is closed, the first measurement in the first reaction pool group 131 is to be tested. The liquid cannot enter the first test liquid preparation section.
  • the sample detecting device 100 further includes an eighth switching member 17, the eighth switching member 17 is disposed on the second conduit 112, and the eighth switching member 17 is located at the Between the fourth access point S4 and the third end portion G3 of the second conduit 112, the eighth switching member 17 is used to implement cutting and communication.
  • the eighth switching member 17 can be a valve.
  • the cutting and connecting of the second conduit 112 is realized by setting the eighth switching member 17, and whether the second liquid to be tested in the second reaction pool group 132 can enter the second liquid preparation portion to be tested.
  • the eighth switching member 17 is turned on, and the second liquid to be tested in the second reaction cell group 132 can enter the second liquid preparation portion; when the eighth switching member 17 is closed, the second second in the second reaction group 132 is to be tested. The liquid cannot enter the second liquid preparation section.
  • the sample detecting device 100 further includes a cleaning assembly 18 to clean the pipeline.
  • the cleaning assembly 18 (the first cleaning component 181, the second cleaning component 182, and the third cleaning component 183) includes a diluent and a cleaning fluid that is stronger than the diluent.
  • the pipeline corresponding to the biological sample is washed by the diluent to wash the blood sample in the pipeline after the reaction.
  • the cleaning action of the cleaning solution may not be for each pipeline, and may be performed for a specific channel, or may be performed after a certain number of runs have been accumulated for a certain pipeline.
  • the sample detecting device 100 further includes a first cleaning component 181 and a second cleaning component 182.
  • the first cleaning component 181 is connected to the first pipeline 111.
  • the cleaning liquid in the first cleaning component 181 flows into the first pipeline 111 to clean the first pipeline 111;
  • the second cleaning component 182 accesses the second pipeline 112,
  • the first The cleaning liquid in the second cleaning unit 182 flows into the second line 112 to clean the second line 112.
  • the first line 111 and the second line 112 are respectively cleaned corresponding to the respective cleaning components 18.
  • the sample detecting component 12 includes a third cleaning component 183, and the first pipeline 111 and the second pipeline 112 are commonly connected to the third cleaning component.
  • the cleaning liquid in the third cleaning component 183 flows into the first pipeline 111 and/or
  • the second line 112 is described to clean the first line 111 and/or the second line 112.
  • the cleaning liquid can simultaneously flow into the first line 111 and the second line 112 for cleaning, and the cleaning liquid can also flow into one of the tubes for cleaning.
  • the third cleaning component 183 is connected to the end of the first pipeline 111; the cleaning fluid in the third cleaning component 183 is from the first pipe.
  • the end of the road 111 flows into the first line 111 to clean the first line 111; and/or the cleaning liquid in the third cleaning unit 183 is from the end of the first line 111 Flowing to the fifth access point S5 of the first pipeline 111, and flowing from the fifth access point S5 into the access point of the second pipeline group 132 at the second pipeline 112,
  • the second line 112 is cleaned. Specifically, the cleaning liquid flows into one of the pipes for cleaning.
  • the detection process of the sample detecting device 100 is different according to the sequence of the first liquid to be tested and the second liquid to be tested.
  • the first liquid to be tested may be detected first
  • the second liquid to be tested may be detected.
  • FIG. 1 Please refer to FIG. 1 as an alternative embodiment: starting the first liquid to be tested: opening the first switching member 1313, the fourth switching member 16 and the seventh switching member 152, the first waiting in the first reaction pool 1311 After the liquid measurement is extracted from the second access point S2 to the first liquid preparation portion to be tested under the negative pressure of the second waste liquid pool 151, the first switching member 1313, the fourth switching member 16, and the seventh switching member are closed. 152. Then, the selection switching member 142 is connected to the fourth branch 143, and the injection liquid in the syringe 141 is pushed from the fourth branch 143 into the third access point S3, and the first liquid in the preparation liquid is prepared.
  • the liquid to be tested is pushed into the first access point S1 and enters the flow chamber 122; at the same time, the fifth switching member 124 and the sixth switching member 126 are opened, and the first liquid to be tested enters the flow chamber 122 from the liquid inlet 122a to be tested, and the sheath liquid
  • the sheath liquid in the pool 123 flows into the flow chamber 122 through the sheath liquid inlet 122b, and the sheath liquid wraps the first liquid to be tested to form a first liquid to be tested for detection; and the liquid to be tested after the last detection Flowing from the outlet 122c into the first waste liquid pool 125, the first test liquid is detected;
  • the second switching member 1323, the eighth switching member 17 and the seventh switching member 152 are opened, and the second liquid to be tested in the second reaction cell 1321 is under the negative pressure of the second waste liquid pool 151.
  • the second switching member 1323, the eighth switching member 17 and the seventh switching member 152 are closed; then the selection switching member 142 and the fifth branch 144 are caused.
  • the injection in the syringe 141 is pushed from the fifth branch 144 into the fourth access point S4, and the second test level is determined.
  • the second liquid to be tested in the backup section is pushed into the first access point S1 and enters the flow chamber 122; at the same time, the fifth switching member 124 and the sixth switching member 126 are opened, and the second liquid to be tested enters from the liquid inlet 122a to be tested.
  • the sheath liquid in the sheath liquid pool 123 flows into the flow chamber 122 through the sheath liquid inlet 122b, and the sheath liquid wraps the second liquid to be tested to form a second liquid to be tested for detection;
  • the second liquid to be tested enters the first waste liquid pool 125 from the outlet 122c, and the second liquid to be tested is detected.
  • the first switching member 1313, the second switching member 1323, the fourth switching member 16, the eighth switching member 17, and the seventh switching member 152 can be simultaneously opened, so that the first liquid to be tested and the second liquid to be tested are simultaneously
  • the first test liquid preparation section and the second test liquid preparation section are respectively entered; then, the selection switching component 142 is switched according to the sequence of the first liquid to be tested and the second liquid to be tested, and details are not described herein again.
  • the second liquid to be tested may be detected first, and then the first liquid to be tested is detected, and the specific process is not described again.
  • the washing process of the cleaning liquid and the diluent are the same, and the specific flushing process of flushing the first line 111 with the cleaning liquid or the diluent is: opening the fourth switching member 16, the seventh switching member 152, in the cleaning liquid or the diluent
  • the positive pressure and the sampling unit 15 under the combined action of the negative pressure power, the cleaning liquid or the diluent enters the first line 111, and the first line 111 is flushed.
  • the specific rinsing process for rinsing the second line 112 by using the cleaning liquid or the diluent is: opening the eighth switching member 17, the seventh switching member 152, and the positive pressure between the cleaning liquid or the diluent and the negative pressure of the sampling assembly 15 Under the action, the cleaning liquid or the diluent enters the second line 112 to rinse the second line 112.
  • a second embodiment of the present invention further provides a sample detecting device 200.
  • the sample detecting device 200 is substantially the same as the sample detecting device 100 provided in the first embodiment, except that the reaction component 23 is further
  • a third reaction cell group 233 for forming a third liquid to be tested is included, the third reaction cell group 233 is connected to a sixth access point S6 of the first conduit 211, and the third reaction cell group 233
  • the liquid to be tested flows to the first access point S1 of the sample needle 221 via the sixth access point S6 of the first conduit 211, so that the liquid to be tested in the third reaction pool group 233 It flows into the sample needle 221 of the detection assembly 22 for detection.
  • the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested may be sequentially accessed into the detecting component 22 according to a sequence of formation to improve the sample detection.
  • the detection speed of the device 200 may be sequentially enter the detecting component according to other preset sequences. 22 to test.
  • the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested may be sequentially input into the detecting component 22 according to a sequence of formation to improve the sample detection.
  • the detection speed of the device 200 may be sequentially input into the detecting component 22 according to a sequence of formation to improve the sample detection.
  • the first liquid to be tested and the third liquid to be tested are both flowed into the first access point S1 via the first conduit 211 for detection. Therefore, the first liquid to be tested and the third liquid to be tested are liquids to be tested which are relatively easy to clean by experiments, and thus the accuracy of the detection can be ensured by cleaning the first pipeline 211.
  • the second liquid to be tested is difficult to clean, and is easy to adhere to the liquid to be tested on the tube wall, and the independent flow of the second liquid to be tested and the first liquid to be tested can prevent cross-contamination and improve the accuracy of detection.
  • the third reaction pool group 233 includes a third reaction pool 2331 , a third branch 2332 , and a third switching member 2333 , where the third reaction pool 2331 is used to form a third to be tested.
  • a third end of the third branch 2332 is connected to the third reaction tank 2331, and the third branch 2332 is connected to the sixth access point S6, and the third switching member 2333 is disposed at
  • the third switching member 2333 is configured to implement cutting and communication of the third branch 2332.
  • the flow direction of the third liquid to be tested is specifically: the third liquid to be tested formed in the third reaction cell 2331 flows into the third branch 2332, and if the third switching member 2333 is opened, the third switching member 2333 realizes the third branch 2332.
  • the third liquid to be tested in the third branch 2332 flows into the sixth access point S6, and flows into the first pipeline 211 through the sixth access point S6; if the third switching member 2333 is closed, the third switching When the second branch 2332 is cut off, the third liquid to be tested in the third branch 2332 cannot flow into the sixth access point S6, and the third liquid to be tested cannot enter the first pipeline 211.
  • the first liquid to be tested preparation section can only enter the third liquid to be tested of the third reaction cell group 233 or the first liquid to be tested in the first reaction cell 231 group 231 at a time.
  • the specific detection process of the sample detecting device 200 when performing the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested is:
  • FIG. 11 starts the first liquid to be tested: opening the first switching member 2313, the fourth switching member 26, and the seventh switching member 252, the first waiting in the first reaction pool 231
  • the measuring liquid is extracted from the second access point S2 to the first liquid preparation section under the negative pressure of the second waste liquid pool 251
  • the first switching member 2313, the fourth switching member 26, and the seventh switching member 252 are closed; then, the selection switching member 242 is connected to the fourth branch 243, and the injection liquid in the syringe 241 is pushed in from the fourth branch 243.
  • the third access point S3 pushes the first liquid to be tested in the first liquid preparation section into the first access point S1 and enters the flow chamber 222; and simultaneously opens the fifth switching member 224 and the sixth switching member 226.
  • the first test liquid enters the flow chamber 222 from the liquid to be tested inlet 222a, and the sheath liquid in the sheath liquid pool 223 flows into the flow chamber 222 through the sheath liquid inlet 222b, and the sheath liquid wraps the first to be tested.
  • the liquid forms a first liquid to be tested for detection; the last detected liquid flow from the outlet 222c enters the first waste liquid pool 225, and the first liquid to be tested is detected;
  • the second liquid to be tested is started, and the second switching member 2323, the eighth switching member 27, and the seventh switching member 252 are opened, and the second liquid to be tested in the second reaction cell 2321 is in the second waste liquid pool.
  • the second switching member 2323, the eighth switching member 27 and the seventh switching member 252 are closed; and then the selection switching member 242 is
  • the fifth branch 244 is connected, the injection liquid in the syringe 241 is pushed from the fifth branch 244 into the fourth access point S4, and the second liquid to be tested in the second liquid preparation section is pushed into the first access.
  • the fifth switching member 224 and the sixth switching member 226 are opened, and the second liquid to be tested enters the flow chamber 222 from the liquid inlet 222a to be tested, and the sheath liquid in the sheath liquid pool 223
  • the sheath liquid inlet 222b flows into the flow chamber 222, and the sheath liquid envelops the second liquid to be tested to form a second liquid to be tested for detection; the last detected liquid flow to be tested enters from the outlet 222c
  • the first waste liquid pool 225, the second liquid to be tested is detected;
  • the third liquid to be tested is started. (Because the third liquid to be tested shares the first liquid preparation portion with the first liquid to be tested, the first pipeline 211 is processed by the cleaning assembly 28 before starting the detection. Cleaning, the specific cleaning steps are the same as those mentioned in the first embodiment, and are not described here again, opening the third switching member 2333, the fourth switching member 26 and the seventh switching member 252, and the third waiting in the third reaction pool 2331 After the liquid measurement is extracted from the sixth access point S6 to the first liquid preparation portion to be tested under the negative pressure of the second waste liquid pool 251, the third switching member 2333, the fourth switching member 26, and the seventh switching member are closed.
  • the selection switching member 242 is connected to the fourth branch 243, and the injection liquid in the syringe 241 is pushed from the fourth branch 243 into the third access point S3, and the third liquid to be tested is prepared in the third section.
  • the liquid to be tested is pushed into the first access point S1 and enters the flow chamber 222; the fifth switching member 224 and the sixth switching member 226 are simultaneously opened, and the third liquid to be tested enters the flow chamber 222 from the liquid inlet 222a to be tested, and the sheath The sheath fluid in the liquid pool 223 flows into the flow through the sheath liquid inlet 222b.
  • the sheath liquid wraps the third liquid to be tested to form a third liquid to be tested for detection; and finally the detected liquid flow from the outlet 222c enters the first waste In the liquid pool 225, the third liquid to be tested is detected.
  • the first switching member 2313 (or the third switching member 2333), the second switching member 2323, the fourth switching member 26, the eighth switching member 27, and the seventh switching member 252 can be simultaneously opened, so that the first to be tested
  • the liquid (or the third liquid to be tested) and the second liquid to be tested simultaneously enter the first liquid preparation section and the second liquid preparation section respectively; and then according to the first liquid to be tested (or the third liquid to be tested) and The sequence of the second liquid to be tested is switched to select the switching member 242, and details are not described herein again.
  • the sample detecting apparatus 200 further includes a fourth reaction pool group 234, and the fourth reaction pool group 234 is connected to the eleventh access point S11 of the first pipeline 211.
  • the liquid to be tested in the fourth reaction cell group 234 flows to the first access point S1 of the sample needle 221 through the eleventh access point S11 of the first conduit 211, so that the fourth reaction
  • the liquid to be tested in the pool group 234 flows into the sample needle 221 of the detecting assembly 22 for detection.
  • the fourth reaction cell group 234 includes a fourth reaction cell 2341, a seventh branch 2342, and an eleventh switching member 2343.
  • the fourth reaction cell 2341 is configured to form a fourth waiting Measuring fluid
  • one end of the seventh branch 2342 is connected to the fourth reaction tank 2341
  • the seventh branch 2342 is in communication with the eleventh access point S11
  • the eleventh switching piece 2343 is disposed on the seventh branch 2342
  • the eleventh switching member 2343 is configured to implement cutting and communication of the seventh branch 2342.
  • the flow direction of the fourth liquid to be tested is specifically: the fourth liquid to be tested formed in the fourth reaction cell 2341 flows into the seventh branch 2342, and if the eleventh switching member 2343 is opened, the eleventh switching member 2343
  • the fourth liquid to be tested in the seventh branch 2342 flows into the eleventh access point S11, and flows into the first pipeline 211 through the eleventh access point S11;
  • the eleventh switching member 2343 is closed, that is, the eleventh switching member 2343 realizes the cutting of the seventh branch 2342, the fourth liquid to be tested in the seventh branch 2342 cannot flow into the eleventh access point S11, and the fourth is to be tested.
  • the liquid cannot enter the first line 211.
  • the sample detecting device 200 is similar to the specific detection process for detecting the fourth liquid to be tested and the third liquid to be tested, and the difference is that the fourth liquid to be tested is different.
  • the flow is as described above. Therefore, the specific detection process of the fourth liquid to be tested is not described here.
  • the sample detecting device 200 provided by the embodiment of the present invention makes the second liquid to be tested and other waiting
  • the independent flow of the test liquid prevents cross-contamination and improves the accuracy of the test.
  • a third embodiment of the present invention further provides a sample detecting device 300.
  • the sample detecting device 300 is substantially the same as the sample detecting device 100 provided in the first embodiment, except that the pipeline assembly 31 is provided.
  • a third line 313 is further included, the reaction assembly 33 further includes a third reaction cell group 333, the third reaction cell group 333 is connected to the third line 313; and the third reaction pool group 333
  • the liquid to be tested flows to the first access point S1 of the sample needle 321 through the third conduit 313, so that the liquid to be tested in the third reaction pool group 333 flows into the sample needle of the detecting component 32. 321 for testing.
  • the third reaction pool group 233 includes a third reaction cell 3331, a third branch 3332, and a third switching member 3333, where the third reaction cell 3331 is used to form a third to be tested.
  • One end of the third branch 3332 is connected to the third reaction tank 3331, and the third branch 3332 is in communication with the third pipeline 313, and the third switching member 3333 is disposed at the On the third branch 3332, the third switching member 3333 is used to implement the cutting and communication of the third branch 3332.
  • the flow direction of the third liquid to be tested is specifically: the third liquid to be tested formed in the third reaction tank 3331 flows into the third branch 3332, and if the third switching member 3333 is opened, the third switching member 3333 realizes the third branch 3332.
  • the third liquid to be tested in the third branch 3332 flows into the third pipeline 313; if the third switching member 3333 is closed, the third switching member 3333 realizes the cutting of the third branch 3332, the third branch
  • the third liquid to be tested in 3332 cannot flow into the third conduit 313, and thus the third liquid to be tested cannot enter the third conduit 313.
  • the first reaction cell group 331, the second reaction cell 3321, and the third reaction cell group 333 are respectively in communication with the first pipe 311, the second pipe 312, and the third pipe 313, so that even in the first reaction zone group
  • the first liquid to be tested in 331, the second liquid to be tested in the second reaction cell group 332, and/or the third liquid to be tested in the third reaction cell group 333 are relatively easy to adhere to the pipe wall, and it is difficult to clean. Because the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested can flow into the detecting component 32 through different pipelines (corresponding to the first pipeline 311, the second pipeline 312, and the third pipeline 313, respectively).
  • the sample needles 321 are detected and do not interfere with each other, which is advantageous for ensuring the accuracy of the detection result of the detecting component 32.
  • connection structure of the third reaction pool group 333 and the third pipeline 313 refer to the communication structure of the second reaction pool 3321 and the second pipeline 312 mentioned in the first embodiment, and details are not described herein again.
  • the structure of the detecting component 32 please refer to the structure of the detecting component 22 mentioned in the first embodiment, and no longer Narration.
  • the liquid to be tested (the first liquid to be tested, the second liquid to be tested, or the third liquid to be tested) enters the flow chamber 322 from the liquid inlet 322a to be tested, and is in the fifth.
  • the switching member 324 is opened, that is, when the sheath liquid pool 323 and the sheath liquid inlet 322b are communicated, the sheath liquid in the sheath liquid pool 323 flows into the flow chamber 322 through the sheath liquid inlet 322b, and the sheath liquid wraps the The liquid to be tested forms a flow of the liquid to be tested for detection.
  • the sixth switching member 326 is opened, that is, when the first waste liquid pool 325 and the outlet 322c are connected, the detected liquid to be tested flows from the outlet 322c. Entering the first waste liquid pool 325.
  • the sample introduction component 34 is configured to test the first test liquid preparation section, the second liquid preparation preparation section or the third test liquid preparation section. The liquid is pushed into the sample needle 321 of the detection assembly 32.
  • the sample introduction component 34 is respectively connected to the third access point S3 of the first pipeline 311, the fourth access point S4 of the second pipeline 312, and the third pipeline.
  • a seventh access point S7 of the third access point S3, the third access point S3 is located between the second access point S2 and the first access point S1, the third access point S3 and the first a channel between the access points S1 is a first liquid to be tested, and a channel between the fourth access point S4 and the first access point S1 is a second liquid to be tested.
  • the channel between the seventh access point S7 and the first access point S1 is a third liquid preparation stage; the liquid to be tested in the first reaction pool 331 group 331 passes through the first pipeline 311.
  • the second access point S2 flows to the third access point S3 of the first pipeline 311, and flows into the first liquid preparation section through the third access point S3 of the first pipeline 311.
  • the liquid to be tested in the second reaction cell 3321 group flows through the second line 312 to the fourth access point S4 of the second line 312, and passes through the second line 312 Four access points S4 flow into the second liquid to be tested preparation section; the third reverse
  • the liquid to be tested in the pool group 333 flows to the seventh access point S7 of the third conduit 313 via the third conduit 313, and flows into the seventh access point S7 of the third conduit 313.
  • the third liquid to be tested is prepared.
  • the push sample assembly 34 includes a syringe 341, a selection switching member 342 disposed at an injection port of the syringe 341, a fourth branch 343, a fifth branch 344, and a sixth branch.
  • One end of the fourth branch 343, the fifth branch 344, and the sixth branch 345 Accessing the third access point S3, the fourth access point S4, and the seventh access point S7, the fourth branch 343, the fifth branch 344, and the sixth
  • the other end of the branch 345 is disposed in the selection switching member 342, and the selection switching member 342 is configured to switch the fourth branch 343, the fifth branch 244, or the sixth branch 345. It is in communication with the syringe 341.
  • the selection switching member 342 can be a selection valve.
  • the injection liquid in the syringe 341 is pushed from the fourth branch 343 into the third access point S3 to prepare the first waiting liquid in the first test liquid.
  • the measuring liquid is pushed into the first access point S1 for detection by the detecting component 32;
  • the injection liquid in the syringe 341 is pushed from the fifth branch 344 into the fourth access point S4 to prepare the second liquid to be tested in the second waiting point.
  • the measuring liquid is pushed into the first access point S1 for detection by the detecting component 32;
  • the injection liquid in the syringe 341 is pushed from the sixth branch 345 into the seventh access point S7 to prepare the third waiting liquid in the third test liquid.
  • the test liquid is pushed into the first access point S1 for detection by the detecting component 23.
  • the selection switching member 342 is disposed such that when the liquid to be tested of the three pipelines of the sample detecting device 300 needs to flow from the first access point S1 into the flow chamber 322 for detection, the switching member 342 can be selected according to the actual required setting.
  • the branch is turned on so that the corresponding liquid to be tested can be pushed into the flow chamber 322 for detection.
  • the sampling component 35 is connected to the first pipeline 311, the second pipeline 312 and/or the third pipeline 313, and the sampling component 35 is used to form a negative Pressing to extract the liquid to be tested in the first reaction cell group 331, the liquid to be tested in the second reaction cell group 332, and/or the liquid to be tested in the third reaction cell group 333
  • the first liquid to be tested preparation section, the second liquid to be tested preparation section, and/or the third liquid to be tested preparation section is connected to the first pipeline 311, the second pipeline 312 and/or the third pipeline 313, and the sampling component 35 is used to form a negative Pressing to extract the liquid to be tested in the first reaction cell group 331, the liquid to be tested in the second reaction cell group 332, and/or the liquid to be tested in the third reaction cell group 333
  • the first liquid to be tested preparation section, the second liquid to be tested preparation section, and/or the third liquid to be tested preparation section are the third liquid to be tested preparation section.
  • the sampling component 35 includes a second waste liquid pool 351 and a seventh switching member 352, a negative pressure is formed in the second waste liquid pool 351, and the seventh switching member 352 is connected to
  • the second waste liquid pool 351 is between the first pipeline 311, the second pipeline 312 and the third pipeline 313, and the seventh switching member 352 is used for cutting and connecting.
  • the seventh switching member 352 can be a valve.
  • the sample detecting device 300 further includes a fourth switching member 36.
  • the fourth switching member 36 refer to the fourth switching member 26 mentioned in the first embodiment, and details are not described herein again.
  • the sample detecting device 300 further includes an eighth switching member 37.
  • the eighth switching member 37 refer to the eighth switching member 27 mentioned in the first embodiment, and details are not described herein again.
  • the corresponding sample detecting device 300 further includes a ninth switching member 39, and the ninth switching member 39 is disposed on the third conduit 313.
  • the ninth switching member 39 is located between the seventh access point S7 and the third conduit 313, and the ninth switching member 39 is configured to implement cutting and communication.
  • the ninth switching member 39 can be a valve.
  • the cutting and connecting of the third conduit 313 is realized by setting the ninth switching member 39, and whether the third liquid to be tested in the third reaction pool group 333 can enter the third liquid preparation portion to be tested.
  • the ninth switching member 39 is turned on, the third liquid to be tested in the third reaction cell group 333 can enter the third liquid preparation portion; the third switching member 39 is closed, and the third third in the third reaction group 333 is tested.
  • the liquid cannot enter the third test liquid preparation section.
  • the sample detecting device 300 further includes a cleaning component 38.
  • the cleaning component 38 on the third conduit 313 refers to the architecture of the cleaning component 28 on the second conduit 212 mentioned in the first embodiment.
  • the first conduit 211, the second conduit 212, and the third conduit 313 can be respectively coupled to the first cleaning assembly 28, the second cleaning assembly 28, and the fourth cleaning assembly 28, such as the first conduit 211, the second.
  • the conduit 212 and the third conduit 313 can be commonly connected to the third cleaning assembly 28 and will not be described herein.
  • the sample detecting device 300 may adopt the following detection process:
  • starting the first liquid to be tested opening the first switching member 3313, the fourth switching member 36, and the seventh switching member 352, the first waiting in the first reaction pool 331
  • the selection switching member 342 is connected to the fourth branch 343, and the injection liquid in the syringe 341 is pushed from the fourth branch 343 into the third access point S3, and the first liquid in the preparation liquid is prepared.
  • the fifth switching member 324 and the sixth switching member 326 are simultaneously opened, and the first liquid to be tested enters the flow chamber 322 from the liquid inlet 322a to be tested, and the sheath
  • the sheath liquid in the liquid pool 323 flows into the flow chamber 322 through the sheath liquid inlet 322b, and the sheath liquid wraps the first liquid to be tested to form a first liquid flow to be tested for detection; Measuring fluid flow from the outlet 322c into the first waste liquid pool 325, The first test liquid is detected;
  • the second liquid to be tested is started, and the second switching member 3323, the eighth switching member 37, and the seventh switching member 352 are opened, and the second liquid to be tested in the second reaction pool 3321 is in the second waste liquid pool.
  • the second switching member 3323, the eighth switching member 37 and the seventh switching member 352 are closed; and then the selection switching member 342 is
  • the fifth branch 344 is connected, the injection liquid in the syringe 341 is pushed from the fifth branch 344 into the fourth access point S4, and the second liquid to be tested in the second liquid preparation section is pushed into the first access.
  • the fifth switching member 324 and the sixth switching member 326 are simultaneously opened, and the second liquid to be tested enters the flow chamber 322 from the liquid inlet 322a to be tested, and the sheath liquid in the sheath liquid pool 323
  • the sheath liquid inlet 322b flows into the flow chamber 322, and the sheath liquid envelops the second liquid to be tested to form a second liquid to be tested for detection; the last detected liquid flow to be tested enters from the outlet 322c
  • the first waste liquid pool 325 is detected by the second liquid to be tested;
  • the third liquid to be tested is started, and the third switching member 3333, the ninth switch 39, and the seventh switching member 352 are opened, and the third liquid to be tested in the third reaction cell 3331 is in the second waste liquid pool 351.
  • the third switching member 3333, the ninth switch 39 and the seventh switching member 352 are closed; then the selection switching member 342 and the sixth are selected.
  • the branch 345 is connected, the injection liquid in the syringe 341 is pushed from the sixth branch 345 into the seventh access point S7, and the third liquid to be tested in the third liquid preparation section is pushed into the first access point S1.
  • the third liquid to be tested After entering the flow chamber 322; simultaneously opening the fifth switching member 324 and the sixth switching member 326, the third liquid to be tested enters the flow chamber 322 from the liquid inlet 322a to be tested, and the sheath liquid in the sheath liquid pool 323 passes through the sheath liquid.
  • the inlet 322b flows into the flow chamber 322, and the sheath liquid wraps the third liquid to be tested to form a third flow to be tested for detection; the third detected liquid flow after the final detection enters from the outlet 322c
  • the first waste liquid pool 325 and the third liquid to be tested are detected.
  • the first switching member 3313, the second switching member 3323, the fourth switching member 36, the eighth switching member 37, the ninth switching 39, and the seventh switching member 352 can be simultaneously opened, so that the first liquid to be tested is
  • the second liquid to be tested and the third liquid to be tested simultaneously enter the first liquid preparation section, the second liquid preparation section and the third liquid preparation section, respectively; and then according to the first liquid to be tested and the second liquid to be tested
  • the sequence of detection switches the selection switching component 342, and details are not described herein again.
  • the pipeline corresponding to the biological sample is washed by the diluent to wash the blood sample in the pipeline after the reaction.
  • Cleaning action of cleaning solution can It is not for each pipeline, it can be cleaned for a specific channel, or it can be cleaned after a certain number of runs have been accumulated for a specific pipeline.
  • the specific rinsing process for rinsing the first line 311 by using the cleaning liquid or the diluent is: opening the fourth switching member 36, the seventh switching member 352, and the negative pressure in the cleaning liquid or the diluent positive pressure and the sampling assembly 35 Under the action, the cleaning liquid or the diluent enters the first line 311 to rinse the first line 311.
  • the specific rinsing process for rinsing the second line 312 by using the cleaning liquid or the diluent is to open the eighth switching member 37 and the seventh switching member 352, and the positive pressure between the cleaning liquid or the diluent and the negative pressure of the sampling assembly 35 are common. Under the action, the cleaning liquid or the diluent enters the second line 312, and the second line 312 is flushed.
  • the specific rinsing process for rinsing the third line 313 by using the cleaning liquid or the diluent is: opening the ninth switching member 39 and the seventh switching member 352, under the combined action of the positive pressure of the cleaning liquid and the negative pressure of the sampling assembly 35, The cleaning liquid enters the third line 313, and the third line 313 is flushed.
  • the embodiment of the present invention further provides a sample analyzer 1000.
  • the sample analyzer 1000 includes a sampling component and a sample detecting device in the first embodiment, the second embodiment or the third embodiment, and the sampling component is configured to collect and allocate biological samples.
  • the sample analyzer 1000 analyzes the biological sample.
  • the sampling component comprises a sampler, the sampler can collect the biological sample outside the outer casing, and then quickly move to the top of the reaction component, and distribute the biological sample to the reaction component for subsequent detection.
  • the sampling component includes a sampler that collects a biological sample and moves over the reaction component to dispense the biological sample into the reaction component for subsequent detection.
  • the sample analyzer 100 further includes a housing including an operating end (left side of FIG. 16), the first reaction pool group and the third reaction pool group relative to the second reaction pool group Arranged close to the operating end.
  • the sample detecting device 300 provided by the embodiment of the present invention enables the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested to pass through different pipelines (corresponding to the first pipeline 311 and the second pipeline 312, respectively).
  • the third conduit 313) flows into the sample needle 321 of the detection component 32 for detection without interfering with each other, which is advantageous for ensuring the accuracy of the detection result of the detection component 32.
  • an embodiment of the present invention further provides a sample detecting method, where the sample detecting method is performed by using the sample detecting device 100 provided in Embodiment 1, and the sample detecting method is used. Including steps 110-150:
  • the first biological sample and the second biological sample are respectively placed in the first reaction pool group 131 and the second reaction pool group 132 to be processed to form a liquid to be tested.
  • the first biological sample is different from the second biological sample.
  • the first set of reaction cells 131 can be used to form a first test solution for detecting a white blood cell count
  • the second set of reaction cells 132 can be used to form a second test liquid for detecting a reticulocyte count.
  • the sampling component 15 extracts the liquid to be tested in the first reaction pool group 131 to the first liquid preparation portion to be tested; and/or the sampling component 15 tests the second reaction pool group 132 to be tested. Extracting liquid into the second liquid preparation section;
  • the first switching member 1313, the fourth switching member 16, the fifth switching member 124, and the seventh switching member 152 are opened, in the first reaction cell 1311.
  • the first liquid to be tested is extracted from the second access point S2 to the first liquid preparation portion to be tested under the negative pressure of the second waste liquid pool 151, and the second liquid to be tested in the second reaction liquid 1321 is in the second
  • the negative pressure of the waste liquid pool 151 is extracted from the second line 112 to the second liquid preparation section to be tested.
  • the sample introduction component 14 pushes the liquid to be tested in the first reaction cell group 131 from the first liquid preparation portion to the first access point S1 of the sample needle 121; or the sample assembly 14
  • the liquid to be tested in the second reaction cell group 132 is pushed into the first access point S1 of the sample needle 121 from the second liquid preparation portion.
  • the first test solution or the second test to be pushed into the first access point S1 according to actual needs, wherein the process of pushing the first test liquid into the first access point S1 is: selecting a switch 142 is connected to the fourth branch 143, and the injection liquid in the syringe 141 is pushed from the fourth branch 143 into the third access point S3, and the first liquid to be tested in the first liquid preparation section is pushed into the first
  • the access point S1 enters the flow cell 122.
  • the process of pushing the second liquid to be tested into the second access point S2 is: the selection switching member 142 is in communication with the fifth branch 144, and the injection liquid in the injector 141 is pushed from the fifth branch 144 into the fourth access point S4. And pushing the second liquid to be tested in the second liquid preparation section into the first access point S1 and entering the flow chamber 122.
  • the detecting component 12 detects the liquid to be tested entering the first access point S1.
  • the sample-injecting component 14 in S130 pushes the liquid to be tested in the first reaction cell group 131 from the first liquid preparation portion to the first access point S1 of the sample needle 121, the first time is The liquid to be tested is detected: the fifth switching member 124 and the sixth switching member 126 are opened, and the first liquid to be tested enters the flow chamber 122 from the liquid inlet 122a to be tested, and the sheath liquid in the sheath liquid pool 123 passes through the sheath liquid inlet.
  • the sheath liquid encloses the first liquid to be tested to form a first flow to be tested for detection; and finally the flow of the test liquid after the detection enters the first flow from the outlet 122c A waste liquid pool 125, the first test liquid is detected.
  • the second test is performed at the second time.
  • the liquid is detected: the fifth switching member 124 and the sixth switching member 126 are opened, and the second liquid to be tested enters the flow chamber 122 from the liquid inlet 122a, and the sheath liquid in the sheath liquid pool 123 flows into the flow through the sheath inlet 122b.
  • the sheath liquid encloses the second liquid to be tested to form a second liquid to be tested for detection; and the second detected liquid flow after the detection enters the first from the outlet 122c
  • the waste liquid pool 125, the second liquid to be tested is detected.
  • the first time is different from the second time, that is, the detecting component 12 detects the first liquid to be tested and the second liquid to be tested at different times (time sharing).
  • the cleaning assembly 18 (the first cleaning component 181, the second cleaning component 182, and the third cleaning component 183) includes a diluent and a cleaning fluid that is stronger than the diluent.
  • the pipeline corresponding to the biological sample is washed by the diluent to wash the blood sample in the pipeline after the reaction.
  • the cleaning action of the cleaning solution may not be for each pipeline, and may be cleaned for a specific channel.
  • the pipeline may be cleaned after one test is completed, or may be performed after a certain pipeline has accumulated a certain number of operations.
  • the first pipeline cleaning liquid may be cleaned after the first liquid to be tested is detected, or the first pipeline may be cleaned with the cleaning fluid after the first pipeline is accumulated for multiple times of detection; After the test liquid is tested, the second pipeline is cleaned. The washing liquid is washed, or the second line is cleaned with the cleaning liquid after the second line is accumulated for multiple times.
  • the washing process of the cleaning liquid and the diluent are the same, and the specific flushing process of flushing the first line 111 with the cleaning liquid or the diluent is: opening the fourth switching member 16, the seventh switching member 152, in the cleaning liquid or the diluent
  • the positive pressure and the sampling unit 15 under the combined action of the negative pressure power, the cleaning liquid or the diluent enters the first line 111, and the first line 111 is flushed.
  • the specific rinsing process for rinsing the second line 112 by using the cleaning liquid or the diluent is: opening the eighth switching member 17, the seventh switching member 152, and the positive pressure between the cleaning liquid or the diluent and the negative pressure of the sampling assembly 15 Under the action, the cleaning liquid or the diluent enters the second line 112 to rinse the second line 112.
  • the first pipeline 111 and the first pipeline are The second line 112 performs cleaning of the cleaning liquid.
  • the sample detecting device 100 further includes a controller, and the controller is configured to count the number of times the detecting component 12 detects the liquid to be tested of the first reaction pool group 131 and the liquid to be tested of the second reaction pool group 132, respectively. And determine whether the number of statistics reaches the preset detection amount.
  • the cleaning strategy is to accumulate a certain number of detections (that is, after the corresponding pipelines are accumulated for a certain number of times), the corresponding pipelines are cleaned, and the detection component 12 performs a preset detection amount on the liquid to be tested of the first reaction pool group 131.
  • the first pipeline 111 is cleaned with a cleaning liquid; and/or, after the detection component 12 detects the preset detection amount of the liquid to be tested of the second reaction pool group 132, the second pipeline is detected. 112 is washed with a cleaning solution.
  • the sample detecting method provided by the embodiment of the present invention enables the first reaction pool group 131 and the second reaction pool group 132 to communicate with the first pipeline 111 and the second pipeline 112, respectively, so that even in the first reaction pool group 131
  • the first liquid to be tested and/or the second liquid to be tested in the second reaction cell group 132 are relatively easy to adhere to the pipe wall, and in the case of being difficult to clean, since the first liquid to be tested and the second liquid to be tested can be different
  • the pipelines (corresponding to the first conduit 111 and the second conduit 112 respectively) flow into the sample needle 121 of the detection assembly 12 for detection, and do not interfere with each other, which is advantageous for ensuring the accuracy of the detection result of the detection component 12.
  • an embodiment of the present invention further provides a sample detecting method, where the sample detecting method is performed by using the sample detecting device 200 provided in the second embodiment, and the sample detecting method includes the following steps:
  • the first biological sample, the second biological sample, and the third biological sample are respectively placed in the first reaction pool group 331, the second reaction pool 332, and the third reaction pool group 233 to be processed to form a test sample. liquid.
  • the first biological sample and the third biological sample are different from the second biological sample; wherein the first biological sample and the third biological sample are the same; or the first biological sample And the third biological sample are each different.
  • the sampling component 25 extracts the liquid to be tested in the first reaction cell group 331 or the liquid to be tested in the third reaction cell group 233 to the first liquid preparation portion to be tested; and/or the sampling component 25
  • the liquid to be tested in the second reaction tank 332 is drawn to the second liquid preparation portion to be tested;
  • the first switching member 2313, the fourth switching member 26, and the seventh switching member 252 are opened, and the first reaction cell 231 is
  • the first liquid to be tested is extracted from the second access point S2 to the first liquid preparation portion after the negative pressure of the second waste liquid pool 251; then the fourth switching member 26 is closed and the eighth switching member 27 is opened.
  • the second liquid to be tested in the second reaction tank 2321 is extracted from the second conduit 212 to the second liquid preparation section under the negative pressure of the second waste liquid tank 251. The sampling of the second liquid to be tested is not described here.
  • the first switching member 2313, the fourth switching member 26, the eighth switching member 27, and the seventh switching member 252 are opened, in the first reaction cell 231.
  • the first liquid to be tested is extracted from the second access point S2 to the first liquid preparation portion to be tested under the negative pressure of the second waste liquid pool 251, and the second liquid to be tested in the second reaction liquid 2321 is in the second
  • the negative pressure of the waste liquid pool 251 is extracted from the second line 212 to the second liquid preparation stage.
  • the third switching member 2333, the fourth switching member 26, the eighth switching member 27, and the seventh switching member 252 are opened, in the third reaction cell 2331
  • the third liquid to be tested is extracted from the sixth access point S6 to the first liquid to be tested under the negative pressure of the second waste liquid pool 251.
  • the second liquid to be tested in the second reaction tank 2321 is extracted from the second conduit 212 to the second liquid preparation section under the negative pressure of the second waste liquid tank 251.
  • the sample-injecting component 24 pushes the liquid to be tested of the first reaction cell 231 from the first liquid preparation portion to the first access point S1 of the sample needle 221; or the second component of the sample assembly 24 The liquid to be tested of the pool 2321 is pushed into the first access point S1 of the sample needle 221 from the second liquid preparation portion to be tested; or the sample assembly 24 is to measure the liquid to be tested of the third reaction pool 2331 from the first A liquid to be measured preparation section is pushed into the first access point S1 of the sample needle 221.
  • the first liquid to be tested, the second liquid to be tested, or the third liquid to be tested are pushed into the first access point S1 according to actual needs, wherein the first liquid to be tested is pushed into the first access point S1.
  • the process is: the selection switching member 242 is in communication with the fourth branch 243, and the injection liquid in the syringe 241 is pushed from the fourth branch 243 into the third access point S3, and the first liquid in the preparation liquid is prepared.
  • the liquid to be tested is pushed into the first access point S1 and enters the flow chamber 222.
  • the process of pushing the second liquid to be tested into the first access point S1 is: the selection switching member 242 is in communication with the fifth branch 244, and the injection liquid in the syringe 241 is pushed from the fifth branch 244 into the fourth access point S4. And pushing the second liquid to be tested in the second liquid preparation section into the first access point S1 and entering the flow chamber 222.
  • the process of pushing the third liquid to be tested into the first access point S1 is: the selection switching member 242 is in communication with the fourth branch 243, and the injection liquid in the syringe 241 is pushed from the fourth branch 243 into the third access point S3.
  • the third liquid to be tested in the first liquid preparation section is pushed into the first access point S1 and then enters the flow chamber 222.
  • the detecting component 22 detects the liquid to be tested entering the first access point S1.
  • the sample-injecting component 24 in S230 pushes the liquid to be tested in the first reaction cell group 331 from the first liquid preparation portion to the first access point S1 of the sample needle 221, the first time is The liquid to be tested is detected: the fifth switching member 224 and the sixth switching member 226 are opened, the first liquid to be tested enters the flow chamber 222 from the liquid inlet 222a to be tested, and the sheath liquid in the sheath liquid pool 223 passes through the sheath liquid.
  • the inlet 222b flows into the flow chamber 222, and the sheath liquid encloses the first liquid to be tested to form a first flow to be tested for detection; and the last detected flow of the test liquid enters the inlet from the outlet 222c The first waste liquid pool 225, the first test liquid is detected.
  • the sample-injecting component 24 in S230 prepares the liquid to be tested in the second reaction cell 332 from the second liquid to be tested
  • the second liquid to be tested is detected at the second time: the fifth switching member 224 and the sixth switching member 226 are opened, and the second liquid to be tested is self-test liquid.
  • the inlet 222a enters the flow chamber 222, and the sheath liquid in the sheath liquid pool 223 flows into the flow chamber 222 through the sheath liquid inlet 222b, and the sheath liquid wraps the second liquid to be tested to form a second liquid to be tested.
  • the second test liquid flow after the last detection enters the first waste liquid pool 225 from the outlet 222c, and the second test liquid is detected.
  • the third test is performed at the third time.
  • the liquid is detected: the fifth switching member 224 and the sixth switching member 226 are opened, the third liquid to be tested enters the flow chamber 222 from the liquid inlet 222a, and the sheath liquid in the sheath liquid pool 223 flows through the sheath liquid inlet 222b.
  • the sheath liquid envelops the third liquid to be tested to form a third liquid to be tested for detection; and finally the detected liquid flow from the outlet 222c enters the first waste In the liquid pool 225, the third liquid to be tested is detected.
  • the first time, the second time, and the third time are different from each other, that is, the detecting component 22 detects the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested at different times (time sharing).
  • the cleaning assembly 28 (the first cleaning assembly 28, the second cleaning assembly 28, and the third cleaning assembly 28) includes a diluent and a cleaning fluid that is stronger than the diluent.
  • the pipeline corresponding to the biological sample is washed by the diluent to wash the blood sample in the pipeline after the reaction.
  • the cleaning action of the cleaning solution may not be for each pipeline, and may be cleaned for a specific channel.
  • the pipeline may be cleaned after one test is completed, or may be performed after a certain pipeline has accumulated a certain number of operations.
  • the first pipeline cleaning liquid may be cleaned after the first liquid to be tested is detected, or the first pipeline may be cleaned with the cleaning fluid after the first pipeline is accumulated for multiple times of detection; After the detection of the liquid to be tested, the second pipeline is cleaned with the cleaning liquid, or after the second pipeline is accumulated for multiple times, the second pipeline is cleaned with the cleaning liquid.
  • the specific rinsing process of rinsing the first pipeline 211 by using the cleaning liquid or the diluent is: opening the fourth switching member 26 and the seventh switching member 252, and the positive pressure and the sampling component 25 are negative in the cleaning liquid or the diluent. Under the action of the pressure power, the cleaning liquid or the diluent enters the first line 211, and the first line 211 Rinse.
  • the specific rinsing process for rinsing the second line 212 by using the cleaning liquid or the diluent is to open the eighth switching member 27 and the seventh switching member 252, and the positive pressure between the cleaning liquid or the diluent and the negative pressure of the sampling assembly 25 are common. Under the action, the cleaning liquid or the diluent enters the second line 212 to rinse the second line 212.
  • the sample detecting method provided by the embodiment of the invention can prevent cross-contamination and improve the accuracy of detection by making the second liquid to be tested flow independently from other liquids to be tested.
  • an embodiment of the present invention further provides a sample detecting method, where the sample detecting method is performed by using the sample detecting device 300 provided in the third embodiment, and the sample detecting method includes the following steps:
  • S310 The first biological sample, the second biological sample, and the third biological sample are respectively placed in the first reaction pool group 331, the second reaction pool 332, and the third reaction pool group 333 to be processed to form a test. liquid.
  • the sampling component 35 extracts the liquid to be tested in the first reaction cell group 331 to the first liquid preparation portion to be tested; and/or the sampling component 35 to measure the liquid to be tested in the second reaction cell 332. Extracting to the second liquid to be tested preparation section; and/or sampling component 35 extracting the liquid to be tested in the third reaction cell group 333 to the third liquid to be tested preparation section.
  • the first switching member 3313, the fourth switching member 36, and the seventh switching member 352 are opened, the first reaction pool
  • the first test liquid in 331 is extracted from the second access point S2 to the first liquid preparation portion after the negative pressure of the second waste liquid pool 351; then the fourth switching member 36 is closed and the eighth is turned on.
  • the switching member 37 the second liquid to be tested in the second reaction tank 3321 is extracted from the second pipeline 312 to the second liquid preparation section under the negative pressure of the second waste liquid pool 351; And the third ninth switch 39 is opened, and the third test liquid in the third reaction tank 3331 is extracted from the third pipe 313 to the third liquid to be tested preparation section under the negative pressure of the second waste liquid pool 351.
  • the sampling order of the first, second and third test liquids can be adjusted as needed, and will not be described again.
  • the first switching member 3313, the fourth switching member 36, the eighth switching member 37, the ninth switching 39, and the seventh are opened.
  • the switching member 352 the first liquid to be tested in the first reaction tank 331 is operated from the second under the negative pressure of the second waste liquid pool 351
  • the point S2 is extracted to the first liquid preparation section to be tested, and the second liquid to be tested in the second reaction tank 3321 is extracted from the second pipeline 312 to the second to be tested under the negative pressure of the second waste liquid pool 351.
  • the third liquid to be tested in the third reaction tank 3331 is extracted from the third pipeline 313 to the third liquid preparation section by the negative pressure of the second waste liquid pool 351.
  • the sample introduction component 34 pushes the liquid to be tested of the first reaction cell 331 from the first liquid preparation portion to the first access point S1 of the sample needle 321; or the sample assembly 34 takes the second reaction.
  • the liquid to be tested of the pool 3321 is pushed into the first access point S1 of the sample needle 321 from the second liquid preparation portion; or the sample assembly 34 is to measure the liquid of the third reaction pool 3331 from the first
  • the third liquid to be measured preparation section is pushed into the first access point S1 of the sample needle 321.
  • the first liquid to be tested, the second liquid to be tested, or the third liquid to be tested are pushed into the first access point S1 according to actual needs, wherein the first liquid to be tested is pushed into the first access point S1.
  • the process is: the selection switching member 342 is in communication with the fourth branch 343, and the injection liquid in the syringe 341 is pushed from the fourth branch 343 into the third access point S3, and the first liquid in the preparation liquid is prepared.
  • the liquid to be tested is pushed into the first access point S1 and enters the flow chamber 322.
  • the process of pushing the second liquid to be tested into the first access point S1 is: the selection switching member 342 is in communication with the fifth branch 344, and the injection liquid in the syringe 341 is pushed from the fifth branch 344 into the fourth access point S4.
  • the second liquid to be tested in the second liquid preparation section is pushed into the first access point S1 and then enters the flow chamber 322.
  • the process of pushing the third liquid to be tested into the first access point S1 is: the selection switching member 342 is connected to the sixth branch 345, and the injection liquid in the syringe 341 is pushed from the sixth branch 345 into the seventh access point S7.
  • the third liquid to be tested in the third liquid preparation section is pushed into the first access point S1 and then enters the flow chamber 322.
  • the detecting component 32 detects the liquid to be tested entering the first access point S1.
  • the sample-injecting component 34 in S330 pushes the liquid to be tested in the first reaction cell group 331 from the first liquid preparation portion to the first access point S1 of the sample needle 321, the first time is The liquid to be tested is detected: the fifth switching member 324 and the sixth switching member 326 are opened, and the first liquid to be tested enters the flow chamber 322 from the liquid inlet 322a to be tested, and the sheath liquid in the sheath liquid pool 323 passes through the sheath liquid.
  • the inlet 322b flows into the flow chamber 322, and the sheath liquid wraps the first liquid to be tested to form a first flow to be tested for detection; the last detected flow of the liquid to be tested enters the inlet from the outlet 322c First waste liquid pool 325, first waiting The liquid test is completed.
  • the second liquid to be tested is in the second time.
  • the detection is performed: the fifth switching member 324 and the sixth switching member 326 are opened, the second liquid to be tested enters the flow chamber 322 from the liquid inlet 322a to be tested, and the sheath liquid in the sheath liquid pool 323 flows into the flow through the sheath liquid inlet 322b.
  • the sheath liquid envelops the second liquid to be tested to form a second liquid to be tested for detection; and the second detected liquid flow after the detection enters the first from the outlet 322c In the waste liquid pool 325, the second liquid to be tested is detected.
  • the third test is performed at the third time.
  • the liquid is detected: the fifth switching member 324 and the sixth switching member 326 are opened, the third liquid to be tested enters the flow chamber 322 from the liquid inlet 322a to be tested, and the sheath liquid in the sheath liquid pool 323 flows through the sheath liquid inlet 322b.
  • the sheath liquid envelops the third liquid to be tested to form a third liquid to be tested for detection; the last detected liquid flow to be tested enters the first waste from the outlet 322c In the liquid pool 325, the third liquid to be tested is detected.
  • the first time, the second time, and the third time are different from each other, that is, the detecting component 32 detects the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested at different times (time sharing).
  • S350 cleaning the first pipeline 311, and/or cleaning the second pipeline 312 and/or cleaning the third pipeline 313.
  • the pipeline corresponding to the biological sample is washed by the diluent to wash the blood sample in the pipeline after the reaction.
  • the cleaning action of the cleaning solution may not be for each pipeline, and may be performed for a specific channel, or may be performed after a certain number of runs have been accumulated for a certain pipeline.
  • the specific rinsing process for rinsing the first line 311 by using the cleaning liquid or the diluent is: opening the fourth switching member 36, the seventh switching member 352, and the negative pressure in the cleaning liquid or the diluent positive pressure and the sampling assembly 35 Under the action, the cleaning liquid or the diluent enters the first line 311 to rinse the first line 311.
  • the specific rinsing process for rinsing the first line 311 by using the cleaning liquid or the diluent is: opening the eighth switching member 37, the seventh switching member 352, and the negative pressure in the cleaning liquid or the diluent positive pressure and the sampling assembly 35 Under the combined action of the power, the cleaning liquid or the diluent enters the second line 312, and the second line 312 is flushed.
  • the specific rinsing process for rinsing the third line 313 by using the cleaning liquid or the diluent is: opening the ninth switch 39, the seventh switching member 352, and cleaning under the combined action of the positive pressure of the cleaning liquid and the negative pressure of the sampling assembly 35.
  • the liquid enters the third line 313 and rinses the third line 313.
  • the sample detecting method provided by the embodiment of the present invention enables the first liquid to be tested, the second liquid to be tested, and the third liquid to be tested to pass through different pipelines (corresponding to the first pipeline 311, the second pipeline 312, and the first The three conduits 313) flow into the sample needles 321 of the detection assembly 32 for detection without interfering with each other, which is advantageous for ensuring the accuracy of the detection results of the detection assembly 32.

Abstract

一种样本检测装置（100，200，300），样本分析仪（1000）和样本检测方法，该样本检测装置（100，200，300）包括：管路组件（11，31），管路组件（11，31）包括第一管路（111，211，311）和第二管路（112，212，312）；检测组件（12，22，32），检测组件（12，22，32）的样本针（121，221，321）包括第一接入点（S1）；反应组件（13，23，33），反应组件（13，23，33）的第一反应池组（131，231，331）接入第一管路（111，211，311）的第二接入点（S2）上，反应组件（13，23，33）的第二反应池组（132，232，332）联通至第二管路（112，212，312）；第一反应池组（131，231，331）中的待测液经第一管路（111，211，311）的第二接入点（S2）流动至样本针（121，221，321）的第一接入点（S1），第二反应池组（132，232，332）中的待测液经第二管路（112，212，312）流动至样本针（121，221，321）的第一接入点（S1）。该样本检测装置（100，200，300）通过将第一反应池组（131，231，331）和第二反应池组（132，232，332）分别接至第一管路（111，211，311）和第二管路（112，212，312）上，使得第一反应池组（131，231，331）和第二反应池组（132，232，332）中的待测液能够从不同的管路推入检测组件（12，22，32）中检测，相互之间不存在干扰。

Description

样本检测装置、样本分析仪及样本检测方法 技术领域
本发明涉及医疗器械技术领域，尤其涉及一种样本检测装置、样本分析仪以及样本检测方法。
背景技术
五分类血液细胞分析仪中核心检测部件为流动室。该检测部件通过光散射法，检测血液细胞的体积、内容物的特异性特征，根据这些特异性特征给出细胞计数结果和各项分类结果。由于试剂系统的发展，血液细胞通过不同试剂处理后，可以得出更多的特异性特征信号，以分辨出更多异常信息，为临床提供有价值的检测结果。
为了实现上述目的，反应系统和检测系统分开两部分，每一种试剂反应在单独的反应池中完成。测量时依次将反应完成的反应物运输到流动室下进行检测，这样就会有反应物直接交叉污染的风险。而现有技术中，为了清洗样本运输过程造成的残留，一般使用稀释液进行冲洗，而由于个别试剂粘附性比较强，因此一般的稀释液冲洗并不能完全将残留物冲洗干净，当下一个样本运输过程中，遇到这些残留物，就会影响样本的纯度，影响测量结果。
发明内容
本发明实施例提供一种样本检测装置、样本分析仪以及样本检测方法。
本发明实施例提供的一种样本检测装置，包括：
管路组件，所述管路组件包括第一管路和第二管路；
检测组件，所述检测组件包括样本针，所述样本针包括第一接入点；
反应组件，所述反应组件包括第一反应池组和第二反应池组，所述第一反应池组接入所述第一管路的第二接入点上，所述第二反应池组联通至所述第二管路，所述反应组件用于对生物样本进行处理以形成待测液；
所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述样本针的第一接入点，所述第二反应池组中的待测液经所述第二管路流动至所 述样本针的第一接入点，以使得所述第一反应池组或所述第二反应池组中的待测液分别流入所述检测组件的样本针中来供检测。
本发明实施例还提供一种样本检测方法，所述样本检测方法样本检测装置进行检测，所述样本检测方法包括：
将第一生物样本和第二生物样本分别置于所述第一反应池组和所述第二反应池组中进行处理以形成待测液；
使所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述样本针的第一接入点，由所述检测组件在第一时间对流入所述样本针的第一接入点的所述第一反应池组的待测液进行检测；
使所述第二反应池组中的待测液经所述第二管路流动至所述样本针的第一接入点，由所述检测组件在第二时间对流入所述样本针的第一接入点的所述第二反应池组的待测液进行检测。
本发明还提供了一种样本分析仪，包括采样组件和样本检测装置，所述采样组件包括采样器，所述采样器采集生物样本，且移动至所述反应组件的上方，以将所述生物样本分配至所述反应组件中。
本发明实施例提供的样本检测装置、样本分析仪和样本检测方法通过将第一反应池组和第二反应池组分别接至第一管路和第二管路上，使得第一反应池组的待测液和第二反应池组的待测液能够从不同的管路推入检测组件中检测，相互之间不存在干扰，有利于保证检测组件检测结果的准确度。
附图说明
为了更清楚地说明本发明的技术方案，下面将对实施方式中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施方式，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以如这些附图获得其他的附图。
图1是本发明实施例一提供的一种样本检测装置的示意图；
图2至图7是图1中的第二管路与第二反应池组的不同联通结构的示意图；
图8至图10是图1中的第二管路与第一接入点的不同联通结构的示意图；
图11是本发明实施例二提供的一种样本检测装置的示意图；
图12是本发明实施例三提供的一种样本检测装置的示意图；
图13是本发明实施例提供的一种样本检测方法的流程图；
图14是本发明实施例提供的另一种样本检测方法的流程图；
图15是本发明实施例提供的再一种样本检测方法的流程图。
图16是本发明的样本分析仪的示意图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
请参照图1，本发明实施例一提供了一种样本检测装置100，包括管路组件11、检测组件12和反应组件13。管路组件11连接于检测组件12和反应组件13之间，管路组件11用于供反应组件13中的待测液流入检测组件12进行检测。
请参照图1，管路组件11包括第一管路111和第二管路112。
检测组件12包括样本针121，样本针121包括第一接入点S1。
反应组件13用于对生物样本进行处理以形成待测液。反应组件13包括用于形成第一待测液的第一反应池组131和用于形成第二待测液的第二反应池组132，第一反应池组131接入第一管路111的第二接入点S2上，第二反应池组132联通至第二管路112；所述第一反应池组131中的待测液经所述第一管路111的第二接入点S2流动至所述样本针121的第一接入点S1，所述第二反应池组132中的待测液经所述第二管路112流动至所述样本针121的第一接入点S1，以使得所述第一反应池组131或所述第二反应池组132中的待测液分别流入所述检测组件12的样本针121中来供检测。
可选的，所述第一待测液和所述第二待测液可以依据形成顺序的先后依序进入所述检测组件12进行检测，以提高所述样本检测装置100的检测速度。当然，在其他实施方式中，所述第一待测液和所述第二待测液也可以依照其他的预设顺序依次进入所述检测组件12进行检测。
可选的，所述第一反应池组131用于形成检测白细胞计数或白细胞分类的第一待测液，所述第二反应池组132用于形成检测网织红细胞计数的第二待测液。
第一反应池组131与第二反应池组132分别与第一管路111和第二管路112的联通，使得即使在第一反应池组131中的第一待测液和/或第二反应池组132中的第二待测液比较容易粘附管壁，不易清洗的情况下，由于第一待测液和第二待测液能够经不同的管路(分别对应第一管路111和第二管路112)流入检测组件12的样本针121来检测，相互之间不干扰，有利于保证检测组件12检测结果的准确度。
可选的，请参照图1，所述第一反应池组131包括第一反应池1311、第一支路1312和第一切换件1313，所述第一反应池1311用于形成第一待测液，所述第一支路1312连接在所述第一反应池1311与所述第二接入点S2之间，所述第一切换件1313设在所述第一支路1312上，所述第一切换件1313用于实现第一支路1312的切断和联通。具体的，第一切换件1313可为阀门。
第一待测液的流向具体为：第一反应池1311中形成的第一待测液流入第一支路1312，若第一切换件1313开启即第一切换件1313实现第一支路1312的联通时，则第一支路1312中的第一待测液流入第二接入点S2，并经第二接入点S2流入第一管路111；若第一切换件1313关闭即第一切换件1313实现第一支路1312的切断时，第一支路1312中的第一待测液无法流入第二接入点S2，进而第一待测液无法进入第一管路111。
可选的，请参照图1，所述第二反应池组132包括第二反应池1321、第二支路1322和第二切换件1323，所述第二反应池1321用于形成第二待测液，所述第二支路1322的一端连接在所述第二反应池1321上，且所述第二支路1322与所述第二管路112联通，所述第二切换件1323设在所述第二支路1322上，所述第二切换件1323用于实现第二支路1322的切断和联通。具体的，第二切换件1323可为阀门。通过第二切换件1323的开启或关闭来实现第二支路1322的联通或切断。
可选的，请参照图2至图7，第二反应池组132与第二管路112的联通结构具体如下所示：
请参照图2，作为一种可选实施例，第二管路112接入第二反应池组132，以使第二反应池组132联通至第二管路112。具体的，所述第二支路1322的一端连接在所述第二反应池1321上，第二支路1322的另一端直接接入第二管路112的第三端部G3。
基于上述第二反应池组132与第二管路112的联通结构，第二反应池组132中的待测液的流向具体为：第二反应池1321中形成的第二待测液流入第二支路1322，若第二切换件1323开启即第二切换件1323实现第二支路1322的联通时，则第二支路1322中的第二待测液流入第二管路112的第三端部G3，进而流入第二管路112；若第二切换件1323关闭即第二切换件1323实现第二支路1322的切断时，则第二支路1322中的第二待测液无法流入第二管路112。
第二管路112与第二反应池组132的联通结构使得第二反应池组132中的待测液能够直接流入第二管路112，不与第一反应池组131流入第一管路111的待测液交叉，并且第二管路112直接接入第二反应池组132的联通结构管路布置较简单，成本较低。
请参照图3，作为一种可选实施例，所述第二反应池组132接入所述第二管路112的第九接入点S9，所述第二反应池组132中的待测液自所述第九接入点S9流入所述第二管路112，以使所述第二反应池组132联通至所述第二管路112。具体的，所述第二支路1322的一端连接在所述第二反应池1321上，第二支路1322的另一端接入第二管路112的第九接入点S9。
基于上述第二反应池组132与第二管路112的联通结构，第二反应池组132中的待测液的流向具体为：第二反应池1321中形成的第二待测液流入第二支路1322，若第二切换件1323开启即第二切换件1323实现第二支路1322的联通时，则第二支路1322中的第二待测液流入第二管路112的第九接入点S9，进而流入第二管路112；若第二切换件1323关闭即第二切换件1323实现第二支路1322的切断时，则第二支路1322中的第二待测液无法流入第九接入点S9。
第二管路112与第二反应池组132的联通结构使得第二反应池组132中的待测液能够直接流入第二管路112，不与第一反应池组131流入第一管路111的待测液交叉，并且第二反应池组132直接接入第二管路112的联通结构管路布置较简单，成本较低。
请参阅图4和图5，作为一种可选实施例，第二反应池组132接入第一管路111的第五接入点S5，第二管路112的一端接入第一管路111的第八接入点S8。具体的，所述第二支路1322的一端连接在所述第二反应池1321上，第二支路1322的另一端先接入第一管路111的第五接入点S5，第二管路112的第三端再接入第一管路111的第八接入点S8。此处第五接入点S5和第八接入点S8较之第二接入点S2离第一接入点S1更远，则使得第一反应池组131中的待测液在经第二接入点S2流动至第一接入点S1的过程中，第一反应池组131中的待测液不会经过第五接入点S5和第八接入点S8，故在第二反应池组132的待测液先经第五接入点S5流动至第八接入点S8直至第二管路112中时，第二反应池组132的待测液同样不会与第一反应池组131中的待测液相互交叉。
可选的，如图4所示，第八接入点S8位于第五接入点S5的右侧。
可选的，如图5所示，第八接入点位于第五接入点S5的左侧。
基于上述第二反应池组132与第二管路112的联通结构，第二反应池组132中的待测液的流向具体为：第二反应池1321中形成的第二待测液流入第二支路1322，若第二切换件1323开启即第二切换件1323实现第二支路1322的联通时，则第二支路1322中的第二待测液流入第五接入点S5，并自第五接入点S5流动至第八接入点S8，以进入第二管路112；若第二切换件1323关闭即第二切换件1323实现第二支路1322的切断时，则第二支路1322中的第二待测液无法流入第五接入点S5。
请参照图6，作为一种可选实施例，第二反应池组132接入第一管路111的第五接入点S5，第二管路112的一端接入第一管路111的第八接入点S8。具体的，所述第二支路1322的一端连接在所述第二反应池1321上，第二支路1322的另一端先接入第一管路111的第五接入点S5，第二管路112的第三端部G3再接入第一管路111的第八接入点S8。此处第五接入点S5和第八接入点S8较之第二接入点S2离第一接入点S1更近。
基于上述第二反应池组132与第二管路112的联通结构，第二反应池组132中的待测液的流向具体为：第二反应池1321中形成的第二待测液流入第二支路1322，若第二切换件1323开启即第二切换件1323实现第二支路1322的联通时，则第二支路1322中的第二待测液流入第五接入点S5，并自第五接 入点S5流动至第八接入点S8，以进入第二管路112；若第二切换件1323关闭即第二切换件1323实现第二支路1322的切断时，则第二支路1322中的第二待测液无法流入第五接入点S5。
对应的，第一反应池组131中的待测液的流向具体为：第一反应池1311中形成的第一待测液流入第一支路1312，若第一切换件1313开启即第一切换件1313实现第一支路1312的联通时，则第一支路1312中的第一待测液流入第二接入点S2，并自第二接入点S2依次流动至第五接入点S5和第八接入点S8，以流入第一接入点S1。其中，第一待测液和第二待测液虽然都会流经第五接入点S5至第八接入点S8，但是第五接入点S5和第八接入点S8之间路程较短，带来的交叉污染可以近似忽略不计。
请参照图7，作为一种可选实施例，所述第二管路112和所述第二反应池组132共同接入所述第一管路111的第五接入点S5，以使所述第二反应池组132联通至所述第二管路112。具体的，所述第二支路1322的一端连接在所述第二反应池1321上，所述第二支路1322的另一端和所述第二管路112的第三端部G3通过四通头(即第五接入点S5)同时接于第一管路111上。
基于上述第二反应池组132与第二管路112的联通结构，第二反应池组132中的待测液的流向具体为：第二反应池1321中形成的第二待测液流入第二支路1322，若第二切换件1323开启即第二切换件1323实现第二支路1322的联通时，则第二支路1322中的第二待测液流入第五接入点S5，并自第五接入点S5流动至第二管路112；若第二切换件1323关闭即第二切换件1323实现第二支路1322的切断时，则第二支路1322中的第二待测液无法流入第五接入点S5。
可选的，请参照图1，所述检测组件12还包括流动室122、鞘液池123、第五切换件124、第一废液池125以及第六切换件126，所述流动室122具有待测液入口122a、鞘液入口122b以及出口122c，所述待测液入口122a连接所述样本针121的第一接入点S1，所述鞘液入口122b连接所述鞘液池123，所述鞘液池123用于存储鞘液，所述第五切换件124连接在所述鞘液池123与所述鞘液入口122b之间，所述第五切换件124用于实现所在通道的切断和联通，所述出口122c连接所述第一废液池125，所述第六切换件126连接在所 述第一废液池125与所述出口122c之间，所述第六切换件126用于实现所在通道的切断和联通。具体的，第五切换件124可为阀门。第六切换件126可为阀门。
可选的，所述鞘液池123连接第三储气罐，两者连通时，第三储气罐提供的第二正压推动所述鞘液流入所述流动室122。
可选的，所述流动室122内设有检测传感器，所述检测传感器用于对所述待测液流进行检测。
可选的，所述第一废液池125连通大气或负压。
请参照图1，流动室122进行检测时，待测液(第一待测液或第二待测液)自待测液入口122a进入所述流动室122，在第五切换件124开启即连通所述鞘液池123与所述鞘液入口122b时，鞘液池123中的鞘液经鞘液入口122b流入流动室122中，且所述鞘液包裹所述待测液形成待测液流以进行检测，在第六切换件126开启即联通所述第一废液池125与所述出口122c时，检测后的所述待测液流自所述出口122c进入所述第一废液池125。
可选的，请参照图8至图10，第二管路112与样本针121的第一接入点S1的联通具体如下所示：
请参照图8，作为一种可选实施例，所述第二管路112的第四端部G4接入所述第一管路111上的第十接入点S10，所述第十接入点S10联通至所述样本针121的第一接入点S1；所述第二反应池组132中的待测液经所述第二管路112流动至所述第一管路111的第十接入点S10，且自所述第一管路111的第十接入点S10流动至所述样本针121的第一接入点S1。可以理解的，所述第一反应池组131中的待测液自所述第一管路111的第二接入点S2流动至所述样本针121的第一接入点S1时，不会流经所述第一管路111上的第十接入点S10，从而避免与第二反应池组132的待测液交叉污染。
第一管路111中的第一待测液流入检测组件12的具体流向为：第一待测液自第二接入点S2流动至第一接入点S1，接着自样本针121的第一接入点S1流入流动室122的待测液入口122a。
第二管路112中的第二待测液流入检测组件12的具体流向为：所述第二待测液自第二管路112流动至第一管路111的第十接入点S10，且自所述第一 管路111的第十接入点S10流动至所述样本针121的第一接入点S1。
本实施例中的第二管路112与样本针121的第一接入点S1的联通结构使得第一管路111和第二管路112中的待测液自第一反应池1311和第二反应池1321中各自独立流入对应的管路，并且再各自独立的流动至样本针121的第一接入点S1，两个待测液唯一有交叉的地方为都需要经过第一接入点S1和样本针121才能进入流动室122进行检测。而根据发明人的实验表明，在进入流动室122时，共用一个接头，交叉污染没有预料的严重，实质上通过两条管路并列设置，已经可以很大程度上避免交叉污染，达到产品设计的要求，即本实施例中的样本检测装置100通过增加一条管路与另一条管路分别独立设置的低成本配置就能够达到较佳的避免交叉污染的效果。
请参照图9，作为一种可选实施例，所述第二管路112的第四端部G4接入所述第一管路111上的第十接入点S10，所述第十接入点S10联通至所述样本针121的第一接入点S1；所述第二反应池组132中的待测液经所述第二管路112流动至所述第一管路111的第十接入点S10，且自所述第一管路111的第十接入点S10流动至所述样本针121的第一接入点S1。可以理解的，所述第一反应池组131中的待测液自所述第一管路111的第二接入点S2流动至所述样本针121的第一接入点S1时，会流经所述第一管路111上的第十接入点S10。该可选实施例中，第十接入点S10靠近第一接入点S1设置。
第一管路111中的第一待测液流入检测组件12的具体流向为：第一待测液自第二接入点S2流动至第十接入点S10，再流动至第一接入点S1，接着自样本针121的第一接入点S1流入流动室122的待测液入口122a。
第二管路112中的第二待测液流入检测组件12的具体流向为：所述第二待测液自第二管路112流动至第一管路111的第十接入点S10，且自所述第一管路111的第十接入点S10流动至所述样本针121的第一接入点S1。
本实施例中的第二管路112与样本针121的第一接入点S1的联通结构使得第一管路111和第二管路112中的待测液在第十接入点S10和第一接入点S1之间具有公共部分，并且两个待测液在进入流动室122时共用一个接头，根据发明人的实验表明，由于第十接入点S10靠近第一接入点S1设置，与第一接入点S1之间的长度很短，所以即使待测液有公共流动部分且共用一个接 头和样本针121，交叉污染没有预料的严重，实质上通过两条管路并列设置，已经可以很大程度上避免交叉污染，达到产品设计的要求，即本实施例中的样本检测装置100通过增加一条管路与另一条管路分别独立设置的低成本配置就能够达到较佳的避免交叉污染的效果。
请参照图10，作为一种可选实施例，所述第二管路112接入所述检测组件12的第一接入点S1。具体的，第二管路112的第四端部G4直接接入第一接入点S1，减少第二待测液的流动路程。
可选的，样本针121、第一管路111和第二管路112通过四通头(第一接入点S1)联通。
第一管路111中的第一待测液流入检测组件12的具体流向为：第一待测液自第二接入点S2流动至第一接入点S1，接着自样本针121的第一接入点S1流入流动室122的待测液入口122a。
第二管路112中的第二待测液流入检测组件12的具体流向为：所述第二待测液自第二管路112流动至第一接入点S1，接着自样本针121的第一接入点S1流入流动室122的待测液入口122a。
本实施例中的第二管路112与样本针121的第一接入点S1的联通结构使得第一管路111和第二管路112中的待测液自第一反应池1311和第二反应池1321中各自独立流入对应的管路，并且再各自独立的流动至样本针121的第一接入点S1，两个待测液唯一有交叉的地方为都需要经过第一接入点S1和样本针121才能进入流动室122进行检测。而根据发明人的实验表明，在进入流动室122时，共用一个接头，交叉污染没有预料的严重，实质上通过两条管路并列设置，已经可以很大程度上避免交叉污染，达到产品设计的要求，即本实施例中的样本检测装置100通过增加一条管路与另一条管路分别独立设置的低成本配置就能够达到较佳的避免交叉污染的效果。
进一步的，请参照图1，所述样本检测装置100还包括推样组件14，通过设置推样组件14来将所述第一待测液准备段或所述第二待测液准备段内的待测液推入所述检测组件12的样本针121中。
所述推样组件14分别接入所述第一管路111的第三接入点S3和所述第二管路112的第四接入点S4，所述第三接入点S3位于所述第二接入点S2和 所述第一接入点S1之间，所述第三接入点S3与所述第一接入点S1之间的通道为第一待测液准备段，所述第四接入点S4与所述第一接入点S1之间的通道为第二待测液准备段；所述第一反应池组131中的待测液经所述第一管路111的第二接入点S2流动至所述第一管路111的第三接入点S3，并经所述第一管路111的第三接入点S3流入所述第一待测液准备段；所述第二反应池组132中的待测液经所述第二管路112流动至所述第二管路112的第四接入点S4，并经所述第二管路112的第四接入点S4流入所述第二待测液准备段。
可选的，请参照图1，所述推样组件14包括注射器141、设置于所述注射器141的注射口的选择切换件142、第四支路143和第五支路144，所述第四支路143和所述第五支路144的一端分别接入所述第三接入点S3和所述第四接入点S4，所述第四支路143和所述第五支路144的另一端共同设在所述选择切换件142，所述选择切换件142用于切换所述第四支路143或所述第五支路144来与所述注射器141联通。具体的，选择切换件142可为选择阀门。
使选择切换件142与第四支路143联通时，则注射器141中的注射液自第四支路143推入第三接入点S3，以将第一待测液准备段内的第一待测液推入第一接入点S1，以供检测组件12检测；
使选择切换件142与第五支路144联通时，则注射器141中的注射液自第五支路144推入第四接入点S4，以将第二待测液准备段内的第二待测液推入第一接入点S1，以供检测组件12检测。
设置选择切换件142，使得样本检测装置100的两个管路的待测液都需要从第一接入点S1流入流动室122中进行检测时，能够根据实际所需设置选择切换件142与对应的支路接通，以使对应的待测液能够被推入流动室122中进行检测。
进一步的，请参照图1，所述样本检测装置100还包括抽样组件15，所述抽样组件15接入所述第一管路111和/或所述第二管路112，所述抽样组件15用于形成负压以将所述第一反应池组131中的待测液和/或所述第二反应池组132中的待测液对应抽取到所述第一待测液准备段和/或所述第二待测液准备段内。
可选的，请参照图1，所述抽样组件15包括第二废液池151和第七切换 件152，所述第二废液池151内形成负压，所述第七切换件152连接在所述第二废液池151与所述第一管路111和所述第二管路112之间，所述第七切换件152用于实现切断和联通。具体的，第七切换件152可为阀门。
可选的，抽样组件15于管路组件11中的连接方式具体如下所示：
如图10所示的第二管路112与第一接入点S1的联通结构，请参照图1和图10，作为一种可选实施例，抽样组件15共同连接于第一管路111和第二管路112的第二端部G2和第四端部G4。具体的，第二废液池151接入第一管路111和第二管路112的端部，第二废液池151内形成负压，打开第七切换件152使第二废液池151与、第一管路111和第二管路112联通，以将第一管路111和/或第二管路112中的待测液抽取到第一待测液准备段和/或第二待测液准备段内。
如图9所示的第二管路112与第一接入点S1的联通结构，请参照图1和图9，作为一种可选实施例，抽样组件15连接于第一管路111的第二端部G2。具体的，第二废液池151接入第一管路111的第二端部G2，第二废液池151内形成负压，打开第七切换件152使第二废液池151与、第一管路111联通，以将第一管路111和/或第二管路112中的待测液抽取到第一待测液准备段和/或第二待测液准备段内。
如图8所示的第二管路112与第一接入点S1的联通结构，请参照图1和图8，作为一种可选实施例，抽样组件15连接于第二管路112。具体的，第二废液池151接入第二管路112，第二废液池151内形成负压，打开第七切换件152使第二废液池151与、第二管路112联通，以将第一管路111和/或第二管路112中的待测液抽取到第一待测液准备段和/或第二待测液准备段内。
可以理解的，图8至图10中的抽样组件15根据第二管路112与第一接入点S1的联通结构的不同而对应设置，上述列举的方式只是作为举例，不局限抽样组件15的其它设置方式。
进一步的，请参照图1，所述样本检测装置100还包括第四切换件16，所述第四切换件16设在所述第一管路111上，所述第四切换件16位于所述第三接入点S3与所述第二接入点S2之间，所述第四切换件16用于实现切断和联通。具体的，第四切换件16可为阀门。通过设置第四切换件16实现第二接入点S2与第三接入点S3之间的切断和联通，控制第一反应池组131中第一待 测液是否能够进入第一待测液准备段。
开启第四切换件16，第一反应池组131中的第一待测液能够进入第一待测液准备段；关闭第四切换件16，则第一反应池组131中的第一待测液无法进入第一待测液准备段。
进一步的，请参照图1，所述样本检测装置100还包括第八切换件17，所述第八切换件17设在所述第二管路112上，所述第八切换件17位于所述第四接入点S4与所述第二管路112的第三端部G3之间，所述第八切换件17用于实现切断和联通。具体的，第八切换件17可为阀门。通过设置第八切换件17实现第二管路112的切断和联通，控制第二反应池组132中第二待测液是否能够进入第二待测液准备段。
开启第八切换件17，第二反应池组132中的第二待测液能够进入第二待测液准备段；关闭第八切换件17，则第二反应池组132中的第二待测液无法进入第二待测液准备段。
进一步的，请参照图1，所述样本检测装置100还包括清洗组件18，以对管路进行清洗。
可选的，清洗组件18(第一清洗组件181、第二清洗组件182和第三清洗组件183)包括稀释液和清洁力度强于稀释液的清洗液。其中，在每完成一个生物样本的检测，则对对应检测生物样本的管路通过稀释液进行清洗，以将反应后的管路中的血样洗掉。清洗液的清洗动作可以并非针对每个管路，可以针对特定的通道进行清洗，也可以是特定管路累积一定运行次数后再进行清洗。
作为一种可选实施例，请参照图3，所述样本检测装置100还包括第一清洗组件181和第二清洗组件182，所述第一清洗组件181接入所述第一管路111，所述第一清洗组件181中的清洗液流入所述第一管路111，以清洗所述第一管路111；所述第二清洗组件182接入所述第二管路112，所述第二清洗组件182中的清洗液流入所述第二管路112，以清洗所述第二管路112。具体的，第一管路111和第二管路112分别对应各自的清洗组件18进行清洗。
作为一种可选实施例，请参照图4，所述样本检测组件12包括第三清洗组件183，所述第一管路111和所述第二管路112共同连接于所述第三清洗组件183上，所述第三清洁组件183中的清洗液流入所述第一管路111和/或所 述第二管路112，以清洗所述第一管路111和/或所述第二管路112。具体的，清洗液能够同时流入第一管路111和第二管路112进行清洗，清洗液还能够流入其中一个管路进行清洗。
作为一种可选实施例，请参照图5，所述第三清洗组件183接入所述第一管路111的端部；所述第三清洗组件183中的清洗液自所述第一管路111的端部流入所述第一管路111，以清洗所述第一管路111；和/或，所述第三清洗组件183中的清洗液自所述第一管路111的端部流至所述第一管路111的第五接入点S5，且自所述第五接入点S5流入所述第二反应池组132于所述第二管路112的接入点，以清洗所述第二管路112。具体的，清洗液流入其中一个管路进行清洗。
样本检测装置100的检测过程根据第一待测液和第二待测液检测的先后而对应不同，例如，可使第一待测液先检测，第二待测液后检测，具体检测过程为：
请参照图1，作为一种可选实施例：开始第一待测液检测：打开第一切换件1313、第四切换件16和第七切换件152，第一反应池1311中的第一待测液在第二废液池151的负压作用下自第二接入点S2被抽取至第一待测液准备段后，关闭第一切换件1313、第四切换件16和第七切换件152；接着使选择切换件142与第四支路143联通，则注射器141中的注射液自第四支路143推入第三接入点S3，将第一待测液准备段内的第一待测液推入第一接入点S1进入流动室122；同时打开第五切换件124和第六切换件126，第一待测液自待测液入口122a进入所述流动室122，鞘液池123中的鞘液经鞘液入口122b流入流动室122中，且所述鞘液包裹所述第一待测液形成第一待测液流以进行检测；最后检测后的所述待测液流自所述出口122c进入所述第一废液池125，第一待测液检测完毕；
开始第二待测液检测，打开第二切换件1323、第八切换件17和第七切换件152，第二反应池1321中的第二待测液在第二废液池151的负压作用下自第二管路112被抽取至第二待测液准备段后，关闭第二切换件1323、第八切换件17和第七切换件152；接着使选择切换件142与第五支路144联通，则注射器141中的注射液自第五支路144推入第四接入点S4，将第二待测液准 备段内的第二待测液推入第一接入点S1进入流动室122后；同时打开第五切换件124和第六切换件126，第二待测液自待测液入口122a进入所述流动室122，鞘液池123中的鞘液经鞘液入口122b流入流动室122中，且所述鞘液包裹所述第二待测液形成第二待测液流以进行检测；最后检测后的所述第二待测液流自所述出口122c进入所述第一废液池125，第二待测液检测完毕。
可选的，可同时打开第一切换件1313、第二切换件1323、第四切换件16、第八切换件17和第七切换件152，使得第一待测液和第二待测液同时分别进入第一待测液准备段和第二待测液准备段；接着根据第一待测液和第二待测液检测的先后顺序切换选择切换件142，在此不再赘述。
可选的，可先检测第二待测液，后检测第一待测液，具体过程不再赘述。
清洗液和稀释液的清洗过程相同，使用清洗液或稀释液对第一管路111进行冲洗的具体的冲洗过程为：打开第四切换件16、第七切换件152，在清洗液或稀释液正压和抽样组件15负压动力共同作用下，清洗液或稀释液进入第一管路111，对第一管路111进行冲洗。
使用清洗液或稀释液对第二管路112进行冲洗的具体的冲洗过程为：打开第八切换件17、第七切换件152，在清洗液或稀释液正压和抽样组件15负压动力共同作用下，清洗液或稀释液进入第二管路112，对第二管路112进行冲洗。
请参照图11，本发明实施例二还提供了一种样本检测装置200，该样本检测装置200与实施例一提供的样本检测装置100大致相同，其不同之处在于，所述反应组件23还包括用于形成第三待测液的第三反应池组233，所述第三反应池组233接入所述第一管路211的第六接入点S6，所述第三反应池组233中的待测液经所述第一管路211的第六接入点S6流动至所述样本针221的第一接入点S1，以使得所述第三反应池组233中的待测液流入所述检测组件22的样本针221中来供检测。
可选的，所述第一待测液、所述第二待测液以及所述第三待测液可以依据形成顺序的先后依序进入所述检测组件22进行检测，以提高所述样本检测装置200的检测速度。当然，在其他实施方式中，所述第一待测液、所述第二待测液以及所述第三待测液也可以依照其他的预设顺序依次进入所述检测组件 22进行检测。
可选的，所述第一待测液、所述第二待测液和所述第三待测液可以依据形成顺序的先后依序进入所述检测组件22进行检测，以提高所述样本检测装置200的检测速度。
可选的，第一待测液与第三待测液都是经第一管路211流入第一接入点S1，以进行检测。故第一待测液与第三待测液为经实验证明较为容易清洗的待测液，如此通过对第一管路211进行清洗，则可保证检测的准确性。而第二待测液则针对较难清洗，容易粘附在管壁的待测液，实现第二待测液与第一待测液的独立流动可防止交叉污染进而提高检测的准确性。
可选的，请参照图11，所述第三反应池组233包括第三反应池2331、第三支路2332和第三切换件2333，所述第三反应池2331用于形成第三待测液，所述第三支路2332的一端连接在所述第三反应池2331上，且所述第三支路2332与所述第六接入点S6联通，所述第三切换件2333设在所述第三支路2332上，所述第三切换件2333用于实现第三支路2332的切断和联通。
第三待测液的流向具体为：第三反应池2331中形成的第三待测液流入第三支路2332，若第三切换件2333开启即第三切换件2333实现第三支路2332的联通时，则第三支路2332中的第三待测液流入第六接入点S6，并经第六接入点S6流入第一管路211；若第三切换件2333关闭即第三切换件2333实现第三支路2332的切断时，第三支路2332中的第三待测液无法流入第六接入点S6，进而第三待测液无法进入第一管路211。
由于第三反应池组233与第一反应池231组231皆为接入第一管路211中，故对应的推样组件24、抽样组件25和清洗组件28与实施例一中的架构相同，在此不再赘述。需要注意的是，第一待测液准备段一次只能供第三反应池组233的第三待测液或第一反应池231组231中的第一待测液进入。
样本检测装置200在进行第一待测液、第二待测液和第三待测液检测时的具体检测过程为：
请参照图11，作为一种可选实施例：开始第一待测液检测：打开第一切换件2313、第四切换件26和第七切换件252，第一反应池231中的第一待测液在第二废液池251的负压作用下自第二接入点S2被抽取至第一待测液准备段 后，关闭第一切换件2313、第四切换件26和第七切换件252；接着使选择切换件242与第四支路243联通，则注射器241中的注射液自第四支路243推入第三接入点S3，将第一待测液准备段内的第一待测液推入第一接入点S1进入流动室222后；同时打开第五切换件224和第六切换件226，第一待测液自待测液入口222a进入所述流动室222，鞘液池时223中的鞘液经鞘液入口222b流入流动室222中，且所述鞘液包裹所述第一待测液形成第一待测液流以进行检测；最后检测后的所述待测液流自所述出口222c进入所述第一废液池225，第一待测液检测完毕；
请参照图11，开始第二待测液检测，打开第二切换件2323、第八切换件27和第七切换件252，第二反应池2321中的第二待测液在第二废液池251的负压作用下自第二管路212被抽取至第二待测液准备段后，关闭第二切换件2323、第八切换件27和第七切换件252；接着使选择切换件242与第五支路244联通，则注射器241中的注射液自第五支路244推入第四接入点S4，将第二待测液准备段内的第二待测液推入第一接入点S1进入流动室222后；同时打开第五切换件224和第六切换件226，第二待测液自待测液入口222a进入所述流动室222，鞘液池时223中的鞘液经鞘液入口222b流入流动室222中，且所述鞘液包裹所述第二待测液形成第二待测液流以进行检测；最后检测后的所述待测液流自所述出口222c进入所述第一废液池225，第二待测液检测完毕；
请参照图11，开始第三待测液检测(由于第三待测液与第一待测液共用第一待测液准备段，故在开始检测前通过清洗组件28对第一管路211进行清洗，具体清洗步骤与实施例一提及的相同，在此不再赘述)，打开第三切换件2333、第四切换件26和第七切换件252，第三反应池2331中的第三待测液在第二废液池251的负压作用下自第六接入点S6被抽取至第一待测液准备段后，关闭第三切换件2333、第四切换件26和第七切换件252；接着使选择切换件242与第四支路243联通，则注射器241中的注射液自第四支路243推入第三接入点S3，将第三待测液准备段内的第三待测液推入第一接入点S1进入流动室222后；同时打开第五切换件224和第六切换件226，第三待测液自待测液入口222a进入所述流动室222，鞘液池时223中的鞘液经鞘液入口222b流入流 动室222中，且所述鞘液包裹所述第三待测液形成第三待测液流以进行检测；最后检测后的所述待测液流自所述出口222c进入所述第一废液池225，第三待测液检测完毕。
可选的，可同时打开第一切换件2313(或第三切换件2333)、第二切换件2323、第四切换件26、第八切换件27和第七切换件252，使得第一待测液(或第三待测液)和第二待测液同时分别进入第一待测液准备段和第二待测液准备段；接着根据第一待测液(或第三待测液)和第二待测液检测的先后顺序切换选择切换件242，在此不再赘述。
进一步的，请参照图11，所述样本检测装置200还包括第四反应池组234，所述第四反应池组234接入所述第一管路211的第十一接入点S11，所述第四反应池组234中的待测液经所述第一管路211的第十一接入点S11流动至所述样本针221的第一接入点S1，以使得所述第四反应池组234中的待测液流入所述检测组件22的样本针221中来供检测。
可选的，请参照图11，所述第四反应池组234包括第四反应池2341、第七支路2342和第十一切换件2343，所述第四反应池2341用于形成第四待测液，所述第七支路2342的一端连接在所述第四反应池2341上，且所述第七支路2342与所述第十一接入点S11联通，所述第十一切换件2343设在所述第七支路2342上，所述第十一切换件2343用于实现第七支路2342的切断和联通。
请参照图11，第四待测液的流向具体为：第四反应池2341中形成的第四待测液流入第七支路2342，若第十一切换件2343开启即第十一切换件2343实现第七支路2342的联通时，则第七支路2342中的第四待测液流入第十一接入点S11，并经第十一接入点S11流入第一管路211；若第十一切换件2343关闭即第十一切换件2343实现第七支路2342的切断时，第七支路2342中的第四待测液无法流入第十一接入点S11，进而第四待测液无法进入第一管路211。
由于第四反应池组234接入第一管路211，故样本检测装置200在检测第四待测液与检测第三待测液的具体检测过程类似，其不同之处在于第四待测液的流向如上所述。故第四待测液的具体检测过程此处不再赘述。
本发明实施例提供的样本检测装置200通过使得第二待测液与其他的待 测液独立流动，可防止交叉污染而提高检测的准确性。
请参照图12，本发明实施例三还提供了一种样本检测装置300，该样本检测装置300与实施例一提供的样本检测装置100大致相同，其不同之处在于，所述管路组件31还包括第三管路313，所述反应组件33还包括第三反应池组333，所述第三反应池组333联通至所述第三管路313；所述第三反应池组333中的待测液经所述第三管路313流动至所述样本针321的第一接入点S1，以使得所述第三反应池组333中的待测液流入所述检测组件32的样本针321中来供检测。
可选的，请参照图12，所述第三反应池组233包括第三反应池3331、第三支路3332和第三切换件3333，所述第三反应池3331用于形成第三待测液，所述第三支路3332的一端连接在所述第三反应池3331上，且所述第三支路3332与所述第三管路313联通，所述第三切换件3333设在所述第三支路3332上，所述第三切换件3333用于实现第三支路3332的切断和联通。
第三待测液的流向具体为：第三反应池3331中形成的第三待测液流入第三支路3332，若第三切换件3333开启即第三切换件3333实现第三支路3332的联通时，则第三支路3332中的第三待测液流入第三管路313；若第三切换件3333关闭即第三切换件3333实现第三支路3332的切断时，第三支路3332中的第三待测液无法流入第三管路313，进而第三待测液无法进入第三管路313。
第一反应池组331、第二反应池3321组和第三反应池组333分别与第一管路311、第二管路312和第三管路313的联通，使得即使在第一反应池组331中的第一待测液、第二反应池组332中的第二待测液和/或第三反应池组333中的第三待测液比较容易粘附管壁，不易清洗的情况下，由于第一待测液、第二待测液和第三待测液能够经不同的管路(分别对应第一管路311、第二管路312和第三管路313)流入检测组件32的样本针321来检测，相互之间不干扰，有利于保证检测组件32检测结果的准确度。
可选的，第三反应池组333与第三管路313的联通结构请参照实施例一中提及的第二反应池3321组与第二管路312的联通结构，在此不再赘述。
检测组件32的结构请参照实施例一提及的检测组件22的结构，在此不再 赘述。
请参照图12，流动室322进行检测时，待测液(第一待测液、第二待测液或第三待测液)自待测液入口322a进入所述流动室322，在第五切换件324开启即连通所述鞘液池时323与所述鞘液入口322b时，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述待测液形成待测液流以进行检测，在第六切换件326开启即联通所述第一废液池325与所述出口322c时，检测后的所述待测液流自所述出口322c进入所述第一废液池325。
可选的，第三管路313与样本针321的第一接入点S1的联通结构请参照实施例一中提及的第二管路312与样本针321的第一接入点S1的联通结构，在此不再赘述。
由于新增第三管路313，故推样组件34用于将所述第一待测液准备段、所述第二待测液准备段或所述第三待测液准备段内的待测液推入所述检测组件32的样本针321中。
请参照图12，所述推样组件34分别接入所述第一管路311的第三接入点S3、所述第二管路312的第四接入点S4和所述第三管路313的第七接入点S7，所述第三接入点S3位于所述第二接入点S2和所述第一接入点S1之间，所述第三接入点S3与所述第一接入点S1之间的通道为第一待测液准备段，所述第四接入点S4与所述第一接入点S1之间的通道为第二待测液准备段，所述第七接入点S7与所述第一接入点S1之间的通道为第三待测液准备段；所述第一反应池331组331中的待测液经所述第一管路311的第二接入点S2流动至所述第一管路311的第三接入点S3，并经所述第一管路311的第三接入点S3流入所述第一待测液准备段；所述第二反应池3321组中的待测液经所述第二管路312流动至所述第二管路312的第四接入点S4，并经所述第二管路312的第四接入点S4流入所述第二待测液准备段；所述第三反应池组333中的待测液经所述第三管路313流动至所述第三管路313的第七接入点S7，并经所述第三管路313的第七接入点S7流入所述第三待测液准备段。
可选的，请参照图12，所述推样组件34包括注射器341、设置于所述注射器341的注射口的选择切换件342、第四支路343、第五支路344和第六支路345，所述第四支路343、所述第五支路344和所述第六支路345的一端分 别接入所述第三接入点S3、所述第四接入点S4和所述第七接入点S7，所述第四支路343、所述第五支路344和所述第六支路345的的另一端共同设在所述选择切换件342，所述选择切换件342用于切换所述第四支路343、所述第五支路244或所述第六支路345的来与所述注射器341联通。具体的，选择切换件342可为选择阀门。
使选择切换件342与第四支路343联通时，则注射器341中的注射液自第四支路343推入第三接入点S3，以将第一待测液准备段内的第一待测液推入第一接入点S1，以供检测组件32检测；
使选择切换件342与第五支路344联通时，则注射器341中的注射液自第五支路344推入第四接入点S4，以将第二待测液准备段内的第二待测液推入第一接入点S1，以供检测组件32检测；
使选择切换件342与第六支路345联通时，则注射器341中的注射液自第六支路345推入第七接入点S7，以将第三待测液准备段内的第三待测液推入第一接入点S1，以供检测组件23检测。
设置选择切换件342，使得样本检测装置300的三个管路的待测液都需要从第一接入点S1流入流动室322中进行检测时，能够根据实际所需设置选择切换件342与对应的支路接通，以使对应的待测液能够被推入流动室322中进行检测。
对应的，请参照图12，所述抽样组件35接入所述第一管路311、所述第二管路312和/或所述第三管路313，所述抽样组件35用于形成负压以将所述第一反应池组331中的待测液、所述第二反应池组332中的待测液和/或所述第三反应池组333中的待测液对应抽取到所述第一待测液准备段、所述第二待测液准备段和/或所述第三待测液准备段内。
可选的，请参照图12，所述抽样组件35包括第二废液池351和第七切换件352，所述第二废液池351内形成负压，所述第七切换件352连接在所述第二废液池351与所述第一管路311、所述第二管路312和所述第三管路313之间，所述第七切换件352用于实现切断和联通。具体的，第七切换件352可为阀门。
进一步的，请参照图12，所述样本检测装置300还包括第四切换件36， 第四切换件36请参照实施例一提及的第四切换件26，在此不再赘述。
进一步的，请参照图12，所述样本检测装置300还包括第八切换件37，第八切换件37请参照实施例一提及的第八切换件27，在此不再赘述。
进一步的，请参照图12，由于新增第三管路313，对应的所述样本检测装置300还包括第九切换件39，所述第九切换件39设在所述第三管路313上，所述第九切换件39位于所述第七接入点S7与所述第三管路313之间，所述第九切换件39用于实现切断和联通。具体的，第九切换件39可为阀门。通过设置第九切换件39实现第三管路313的切断和联通，控制第三反应池组333中第三待测液是否能够进入第三待测液准备段。
开启第九切换件39，第三反应池组333中的第三待测液能够进入第三待测液准备段；关闭第九切换件39，则第三反应池组333中的第三待测液无法进入第三待测液准备段。
进一步的，请参照图12，所述样本检测装置300还包括清洗组件38，第三管路313上的清洗组件38请参照实施例一提及的第二管路212上的清洗组件28的架构，例如第一管路211、第二管路212和第三管路313可分别联通至第一清洗组件28、第二清洗组件28和第四清洗组件28，例如第一管路211、第二管路212和第三管路313可共同联通至第三清洗组件28，在此不再赘述。
样本检测装置300在检测第一待测液、第二待测液和第三待测液时，可采用如下检测过程：
请参照图12，作为一种可选实施例：开始第一待测液检测：打开第一切换件3313、第四切换件36和第七切换件352，第一反应池331中的第一待测液在第二废液池351的负压作用下自第二接入点S2被抽取至第一待测液准备段后，关闭第一切换件3313、第四切换件36和第七切换件352；接着使选择切换件342与第四支路343联通，则注射器341中的注射液自第四支路343推入第三接入点S3，将第一待测液准备段内的第一待测液推入第一接入点S1进入流动室322后；同时打开第五切换件324和第六切换件326，第一待测液自待测液入口322a进入所述流动室322，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述第一待测液形成第一待测液流以进行检测；最后检测后的所述待测液流自所述出口322c进入所述第一废液池325， 第一待测液检测完毕；
请参照图12，开始第二待测液检测，打开第二切换件3323、第八切换件37和第七切换件352，第二反应池3321中的第二待测液在第二废液池351的负压作用下自第二管路312被抽取至第二待测液准备段后，关闭第二切换件3323、第八切换件37和第七切换件352；接着使选择切换件342与第五支路344联通，则注射器341中的注射液自第五支路344推入第四接入点S4，将第二待测液准备段内的第二待测液推入第一接入点S1进入流动室322后；同时打开第五切换件324和第六切换件326，第二待测液自待测液入口322a进入所述流动室322，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述第二待测液形成第二待测液流以进行检测；最后检测后的所述待测液流自所述出口322c进入所述第一废液池325，第二待测液检测完毕；
请参照图12，开始第三待测液检测，打开第三切换件3333、第九切换39和第七切换件352，第三反应池3331中的第三待测液在第二废液池351的负压作用下自第三管路313被抽取至第三待测液准备段后，关闭第三切换件3333、第九切换39和第七切换件352；接着使选择切换件342与第六支路345联通，则注射器341中的注射液自第六支路345推入第七接入点S7，将第三待测液准备段内的第三待测液推入第一接入点S1进入流动室322后；同时打开第五切换件324和第六切换件326，第三待测液自待测液入口322a进入所述流动室322，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述第三待测液形成第三待测液流以进行检测；最后检测后的所述第三待测液流自所述出口322c进入所述第一废液池325，第三待测液检测完毕。
可选的，可同时打开第一切换件3313、第二切换件3323、第四切换件36、第八切换件37、第九切换39和第七切换件352，使得第一待测液、第二待测液和第三待测液同时分别进入第一待测液准备段、第二待测液准备段和第三待测液准备段；接着根据第一待测液和第二待测液检测的先后顺序切换选择切换件342，在此不再赘述。
可选的，在每完成一个生物样本的检测，则对对应检测生物样本的管路通过稀释液进行清洗，以将反应后的管路中的血样洗掉。清洗液的清洗动作可以 并非针对每个管路，可以针对特定的通道进行清洗，也可以是特定管路累积一定运行次数后再进行清洗。
使用清洗液或稀释液对第一管路311进行冲洗的具体的冲洗过程为：打开第四切换件36、第七切换件352，在清洗液或稀释液正压和抽样组件35负压动力共同作用下，清洗液或稀释液进入第一管路311，对第一管路311进行冲洗。
使用清洗液或稀释液对第二管路312进行冲洗的具体的冲洗过程为：打开第八切换件37、第七切换件352，在清洗液或稀释液正压和抽样组件35负压动力共同作用下，清洗液或稀释液进入第二管路312，对第二管路312进行冲洗。
使用清洗液或稀释液对第三管路313进行冲洗的具体的冲洗过程为：打开第九切换件39、第七切换件352，在清洗液正压和抽样组件35负压动力共同作用下，清洗液进入第三管路313，对第三管路313进行冲洗。
本发明实施例还提供一种样本分析仪1000，样本分析仪1000包括采样组件和实施例一、实施例二或实施例三中的样本检测装置，采样组件用于采集和分配生物样本。样本分析仪1000对生物样本进行分析。其中，采样组件包括采样器，采样器可在外壳的外部采集生物样本，而后快速移动至反应组件的上方，将生物样本分配至反应组件以进行后续检测。其中，所述采样组件包括采样器，所述采样器采集生物样本，且移动至所述反应组件的上方，以将所述生物样本分配至所述反应组件中，以进行后续的检测。
可以理解的，样本分析仪100还包括外壳，所述外壳包括操作端(图16的左侧)，所述第一反应池组和所述第三反应池组相对于所述第二反应池组靠近所述操作端排布。
本发明实施例提供的样本检测装置300通过使得第一待测液、第二待测液和第三待测液能够经不同的管路(分别对应第一管路311、第二管路312和第三管路313)流入检测组件32的样本针321来检测，相互之间不干扰，有利于保证检测组件32检测结果的准确度。
请参照图1和图13，本发明实施例还提供一种样本检测方法，所述样本检测方法采用实施例一提供的样本检测装置100进行检测，所述样本检测方法 包括步骤110-150：
S110：将第一生物样本和第二生物样本分别置于所述第一反应池组131和所述第二反应池组132中进行处理以形成待测液。
可选的，所述第一生物样本不同于所述第二生物样本。例如，所述第一反应池组131可用于形成检测白细胞计数的第一待测液，所述第二反应池组132可用于形成检测网织红细胞计数的第二待测液。
S120：抽样组件15将所述第一反应池组131中的待测液抽取到所述第一待测液准备段；和/或抽样组件15将所述第二反应池组132中的待测液抽取到所述第二待测液准备段；
具体为，当依次对第一待测液和第二待测液进行抽样时：打开第一切换件1313、第四切换件16和第七切换件152，第一反应池1311中的第一待测液在第二废液池151的负压作用下自第二接入点S2被抽取至第一待测液准备段后，关闭第一切换件1313、第四切换件16和第七切换件152。此处不再对第二待测液先抽样进行赘述。
当同时对第一待测液和第二待测液进行抽样时，打开第一切换件1313、第四切换件16、第五切换件124和第七切换件152，第一反应池1311中的第一待测液在第二废液池151的负压作用下自第二接入点S2被抽取至第一待测液准备段，第二反应池1321中的第二待测液在第二废液池151的负压作用下自第二管路112被抽取至第二待测液准备段。
上述两种抽样的情况根据实际所需而对应进行，在待测液抽样完成后，将打开的切换件关闭，以便于后续操作。
S130：推样组件14将第一反应池组131中的待测液自所述第一待测液准备段推入所述样本针121的第一接入点S1；或推样组件14将第二反应池组132中的待测液自所述第二待测液准备段推入所述样本针121的第一接入点S1；
具体为，根据实际所需对应将第一待测液或第二待测推入第一接入点S1，其中，第一待测液推入第一接入点S1的过程为：选择切换件142与第四支路143联通，则注射器141中的注射液自第四支路143推入第三接入点S3，将第一待测液准备段内的第一待测液推入第一接入点S1后进入流动室122。
第二待测液推入第二接入点S2的过程为：选择切换件142与第五支路144联通，则注射器141中的注射液自第五支路144推入第四接入点S4，将第二待测液准备段内的第二待测液推入第一接入点S1后进入流动室122。
S140:检测组件12对进入所述第一接入点S1的待测液进行检测。
具体的，若S130中推样组件14将第一反应池组131中的待测液自第一待测液准备段推入样本针121的第一接入点S1，则在第一时间对第一待测液进行检测：打开第五切换件124和第六切换件126，第一待测液自待测液入口122a进入所述流动室122，鞘液池123中的鞘液经鞘液入口122b流入流动室122中，且所述鞘液包裹所述第一待测液形成第一待测液流以进行检测；最后检测后的所述待测液流自所述出口122c进入所述第一废液池125，第一待测液检测完毕。
若S130中推样组件14将第二反应池组132中的待测液自第二待测液准备段推入样本针121的第一接入点S1，则在第二时间对第二待测液进行检测：打开第五切换件124和第六切换件126，第二待测液自待测液入口122a进入所述流动室122，鞘液池123中的鞘液经鞘液入口122b流入流动室122中，且所述鞘液包裹所述第二待测液形成第二待测液流以进行检测；最后检测后的所述第二待测液流自所述出口122c进入所述第一废液池125，第二待测液检测完毕。
以上第一时间不同于第二时间，即检测组件12在不同时间分别(分时)对第一待测液和第二待测液进行检测。
S150：对所述第一管路111进行清洗和/或对所述第二管路112进行清洗。
可选的，清洗组件18(第一清洗组件181、第二清洗组件182和第三清洗组件183)包括稀释液和清洁力度强于稀释液的清洗液。其中，在每完成一个生物样本的检测，则对对应检测生物样本的管路通过稀释液进行清洗，以将反应后的管路中的血样洗掉。清洗液的清洗动作可以并非针对每个管路，可以针对特定的通道进行清洗，可以在一次检测完毕后对相应管路进行清洗，也可以是特定管路累积一定运行次数后再进行清洗。例如，可以在第一待测液检测完毕后对第一管路用清洗液进行清洗，或者在第一管路累积多次检测完毕之后再用清洗液对第一管路进行清洗；可以在第二待测液检测完毕后对第二管路用清 洗液进行清洗，或者在第二管路累积多次检测完毕之后再用清洗液对第二管路进行清洗。
清洗液和稀释液的清洗过程相同，使用清洗液或稀释液对第一管路111进行冲洗的具体的冲洗过程为：打开第四切换件16、第七切换件152，在清洗液或稀释液正压和抽样组件15负压动力共同作用下，清洗液或稀释液进入第一管路111，对第一管路111进行冲洗。
使用清洗液或稀释液对第二管路112进行冲洗的具体的冲洗过程为：打开第八切换件17、第七切换件152，在清洗液或稀释液正压和抽样组件15负压动力共同作用下，清洗液或稀释液进入第二管路112，对第二管路112进行冲洗。
若第一待测液和第二待测液为依次进行检测，也可在第一待测液和第二待测液均进行一次或累积次数的检测后，再对第一管路111和第二管路112进行清洗液的清洗。
所述样本检测装置100还包括控制器，所述控制器可用于统计所述检测组件12分别对第一反应池组131的待测液和第二反应池组132的待测液进行检测的次数，并判断统计的该次数是否达到预设检测量。当清洗策略为累积一定检测次数后(即相应管路累积运行一定次数后)再对相应管路进行清洗时，在检测组件12对第一反应池组131的待测液进行预设检测量的检测后，对所述第一管路111用清洗液进行清洗；和/或，在检测组件12对第二反应池组132的待测液进行预设检测量的检测后，对第二管路112用清洗液进行清洗。
本发明实施例提供的样本检测方法通过使得第一反应池组131与第二反应池组132分别与第一管路111和第二管路112的联通，使得即使在第一反应池组131中的第一待测液和/或第二反应池组132中的第二待测液比较容易粘附管壁，不易清洗的情况下，由于第一待测液和第二待测液能够经不同的管路(分别对应第一管路111和第二管路112)流入检测组件12的样本针121来检测，相互之间不干扰，有利于保证检测组件12检测结果的准确度。
请参照图11和图14，本发明实施例还提供一种样本检测方法，所述样本检测方法采用实施例二提供的样本检测装置200进行检测，所述样本检测方法包括步骤：
S210：将第一生物样本、第二生物样本和第三生物样本分别置于所述第一反应池组331、所述第二反应池332和第三反应池组233中进行处理以形成待测液。
可选的，所述第一生物样本和第三生物样本均不同于所述第二生物样本；其中，所述第一生物样本和所述第三生物样本相同；或者，所述第一生物样本和所述第三生物样本各自不同。
S220：抽样组件25将所述第一反应池组331中的待测液或第三反应池组233中的待测液抽取到所述第一待测液准备段；和/或抽样组件25将所述第二反应池332中的待测液抽取到所述第二待测液准备段；
具体为，当先对第一待测液进行抽样、后对第二待测液进行抽样时：打开第一切换件2313、第四切换件26和第七切换件252，第一反应池231中的第一待测液在第二废液池251的负压作用下自第二接入点S2被抽取至第一待测液准备段后；接着关闭第四切换件26和打开第八切换件27，第二反应池2321中的第二待测液在第二废液池251的负压作用下自第二管路212被抽取至第二待测液准备段。此处不再对第二待测液先抽样进行赘述。
当先对第三待测液进行抽样、后对第二待测液进行抽样时：打开第三切换件2333、第四切换件26和第七切换件252，第三反应池2331中的第三待测液在第二废液池251的负压作用下自第六接入点S6被抽取至第一待测液准备段后，接着关闭第四切换件26和打开第八切换件27，第二反应池2321中的第二待测液在第二废液池251的负压作用下自第二管路212被抽取至第二待测液准备段。此处不再对第二待测液先抽样进行赘述。
当同时对第一待测液和第二待测液进行抽样时，打开第一切换件2313、第四切换件26、第八切换件27和第七切换件252，第一反应池231中的第一待测液在第二废液池251的负压作用下自第二接入点S2被抽取至第一待测液准备段，第二反应池2321中的第二待测液在第二废液池251的负压作用下自第二管路212被抽取至第二待测液准备段。
当同时对第三待测液和第二待测液进行抽样时，打开第三切换件2333、第四切换件26、第八切换件27和第七切换件252，第三反应池2331中的第三待测液在第二废液池251的负压作用下自第六接入点S6被抽取至第一待测液 准备段，第二反应池2321中的第二待测液在第二废液池251的负压作用下自第二管路212被抽取至第二待测液准备段。
上述四种抽样的情况根据实际所需而对应进行，在待测液抽样完成后，将打开的切换件对应关闭，以便于后续操作。
S230：推样组件24将第一反应池231的待测液自所述第一待测液准备段推入所述样本针221的第一接入点S1；或推样组件24将第二反应池2321的待测液自所述第二待测液准备段推入所述样本针221的第一接入点S1；或推样组件24将第三反应池2331的待测液自所述第一待测液准备段推入所述样本针221的第一接入点S1。
具体为，根据实际所需对应将第一待测液、第二待测液或第三待测液推入第一接入点S1，其中，第一待测液推入第一接入点S1的过程为：选择切换件242与第四支路243联通，则注射器241中的注射液自第四支路243推入第三接入点S3，将第一待测液准备段内的第一待测液推入第一接入点S1后进入流动室222。
第二待测液推入第一接入点S1的过程为：选择切换件242与第五支路244联通，则注射器241中的注射液自第五支路244推入第四接入点S4，将第二待测液准备段内的第二待测液推入第一接入点S1后进入流动室222。
第三待测液推入第一接入点S1的过程为：选择切换件242与第四支路243联通，则注射器241中的注射液自第四支路243推入第三接入点S3，将第一待测液准备段内的第三待测液推入第一接入点S1后进入流动室222。
S240:检测组件22对进入所述第一接入点S1的待测液进行检测。
具体的，若S230中推样组件24将第一反应池组331中的待测液自第一待测液准备段推入样本针221的第一接入点S1，则在第一时间对第一待测液进行检测：打开第五切换件224和第六切换件226，第一待测液自待测液入口222a进入所述流动室222，鞘液池时223中的鞘液经鞘液入口222b流入流动室222中，且所述鞘液包裹所述第一待测液形成第一待测液流以进行检测；最后检测后的所述待测液流自所述出口222c进入所述第一废液池225，第一待测液检测完毕。
若S230中推样组件24将第二反应池332中的待测液自第二待测液准备 段推入样本针221的第一接入点S1，则在第二时间对第二待测液进行检测：打开第五切换件224和第六切换件226，第二待测液自待测液入口222a进入所述流动室222，鞘液池时223中的鞘液经鞘液入口222b流入流动室222中，且所述鞘液包裹所述第二待测液形成第二待测液流以进行检测；最后检测后的所述第二待测液流自所述出口222c进入所述第一废液池225，第二待测液检测完毕。
若S230中推样组件24将第三反应池组233中的待测液自第一待测液准备段推入样本针221的第一接入点S1，则在第三时间对第三待测液进行检测：打开第五切换件224和第六切换件226，第三待测液自待测液入口222a进入所述流动室222，鞘液池时223中的鞘液经鞘液入口222b流入流动室222中，且所述鞘液包裹所述第三待测液形成第三待测液流以进行检测；最后检测后的所述待测液流自所述出口222c进入所述第一废液池225，第三待测液检测完毕。
以上第一时间、第二时间和第三时间各自不同，即检测组件22在不同时间分别(分时)对第一待测液、第二待测液和第三待测液进行检测。
S250：对所述第一管路211进行清洗和/或对所述第二管路212进行清洗。
可选的，清洗组件28(第一清洗组件28、第二清洗组件28和第三清洗组件28)包括稀释液和清洁力度强于稀释液的清洗液。其中，在每完成一个生物样本的检测，则对对应检测生物样本的管路通过稀释液进行清洗，以将反应后的管路中的血样洗掉。清洗液的清洗动作可以并非针对每个管路，可以针对特定的通道进行清洗，可以在一次检测完毕后对相应管路进行清洗，也可以是特定管路累积一定运行次数后再进行清洗。例如，可以在第一待测液检测完毕后对第一管路用清洗液进行清洗，或者在第一管路累积多次检测完毕之后再用清洗液对第一管路进行清洗；可以在第二待测液检测完毕后对第二管路用清洗液进行清洗，或者在第二管路累积多次检测完毕之后再用清洗液对第二管路进行清洗。
具体为，使用清洗液或稀释液对第一管路211进行冲洗的具体的冲洗过程为：打开第四切换件26、第七切换件252，在清洗液或稀释液正压和抽样组件25负压动力共同作用下，清洗液或稀释液进入第一管路211，对第一管路211 进行冲洗。
使用清洗液或稀释液对第二管路212进行冲洗的具体的冲洗过程为：打开第八切换件27、第七切换件252，在清洗液或稀释液正压和抽样组件25负压动力共同作用下，清洗液或稀释液进入第二管路212，对第二管路212进行冲洗。
本发明实施例提供的样本检测方法通过使得第二待测液与其他的待测液独立流动，可防止交叉污染而提高检测的准确性。
请参照图12和图15，本发明实施例还提供一种样本检测方法，所述样本检测方法采用实施例三提供的样本检测装置300进行检测，所述样本检测方法包括步骤：
S310：将第一生物样本、第二生物样本和第三生物样本分别置于所述第一反应池组331、所述第二反应池332和第三反应池组333中进行处理以形成待测液。
S320：抽样组件35将所述第一反应池组331中的待测液抽取到所述第一待测液准备段；和/或抽样组件35将所述第二反应池332中的待测液抽取到所述第二待测液准备段；和/或抽样组件35将第三反应池组333中的待测液抽取到所述第三待测液准备段。
具体为，当依次对第一待测液、第二待测液和第三待测液进行抽样时：打开第一切换件3313、第四切换件36和第七切换件352，第一反应池331中的第一待测液在第二废液池351的负压作用下自第二接入点S2被抽取至第一待测液准备段后；接着关闭第四切换件36和打开第八切换件37，第二反应池3321中的第二待测液在第二废液池351的负压作用下自第二管路312被抽取至第二待测液准备段；再关闭第八切换件37和打开第九切换39，第三反应池3331中的第三待测液在第二废液池351的负压作用下自第三管路313被抽取至第三待测液准备段。第一、第二和第三待测液的抽样顺序可依需调整，不再赘述。
当同时对第一待测液、第二待测液和第三待测液进行抽样时，打开第一切换件3313、第四切换件36、第八切换件37、第九切换39和第七切换件352，第一反应池331中的第一待测液在第二废液池351的负压作用下自第二接入 点S2被抽取至第一待测液准备段，第二反应池3321中的第二待测液在第二废液池351的负压作用下自第二管路312被抽取至第二待测液准备段，第三反应池3331中的第三待测液在第二废液池351的负压作用下自第三管路313被抽取至第三待测液准备段。
上述四种抽样的情况根据实际所需而对应进行，在待测液抽样完成后，将打开的切换件对应关闭，以便于后续操作。
S330：推样组件34将第一反应池331的待测液自所述第一待测液准备段推入所述样本针321的第一接入点S1；或推样组件34将第二反应池3321的待测液自所述第二待测液准备段推入所述样本针321的第一接入点S1；或推样组件34将第三反应池3331的待测液自所述第三待测液准备段推入所述样本针321的第一接入点S1。
具体为，根据实际所需对应将第一待测液、第二待测液或第三待测液推入第一接入点S1，其中，第一待测液推入第一接入点S1的过程为：选择切换件342与第四支路343联通，则注射器341中的注射液自第四支路343推入第三接入点S3，将第一待测液准备段内的第一待测液推入第一接入点S1后进入流动室322。
第二待测液推入第一接入点S1的过程为：选择切换件342与第五支路344联通，则注射器341中的注射液自第五支路344推入第四接入点S4，将第二待测液准备段内的第二待测液推入第一接入点S1后进入流动室322。
第三待测液推入第一接入点S1的过程为：选择切换件342与第六支路345联通，则注射器341中的注射液自第六支路345推入第七接入点S7，将第三待测液准备段内的第三待测液推入第一接入点S1后进入流动室322。
S340:检测组件32对进入所述第一接入点S1的待测液进行检测。
具体的，若S330中推样组件34将第一反应池组331中的待测液自第一待测液准备段推入样本针321的第一接入点S1，则在第一时间对第一待测液进行检测：打开第五切换件324和第六切换件326，第一待测液自待测液入口322a进入所述流动室322，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述第一待测液形成第一待测液流以进行检测；最后检测后的所述待测液流自所述出口322c进入所述第一废液池325，第一待 测液检测完毕。
若S330中推样组件34将第二反应池332中的待测液自第二待测液准备段推入样本针321的第一接入点S1，则在第二时间对第二待测液进行检测：打开第五切换件324和第六切换件326，第二待测液自待测液入口322a进入所述流动室322，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述第二待测液形成第二待测液流以进行检测；最后检测后的所述第二待测液流自所述出口322c进入所述第一废液池325，第二待测液检测完毕。
若S330中推样组件34将第三反应池组333中的待测液自第三待测液准备段推入样本针321的第一接入点S1，则在第三时间对第三待测液进行检测：打开第五切换件324和第六切换件326，第三待测液自待测液入口322a进入所述流动室322，鞘液池时323中的鞘液经鞘液入口322b流入流动室322中，且所述鞘液包裹所述第三待测液形成第三待测液流以进行检测；最后检测后的所述待测液流自所述出口322c进入所述第一废液池325，第三待测液检测完毕。
以上第一时间、第二时间和第三时间各自不同，即检测组件32在不同时间分别(分时)对第一待测液、第二待测液和第三待测液进行检测。
S350：对所述第一管路311进行清洗、和/或对所述第二管路312进行清洗和/或对所述第三管路313进行清洗。
可选的，在每完成一个生物样本的检测，则对对应检测生物样本的管路通过稀释液进行清洗，以将反应后的管路中的血样洗掉。清洗液的清洗动作可以并非针对每个管路，可以针对特定的通道进行清洗，也可以是特定管路累积一定运行次数后再进行清洗。
使用清洗液或稀释液对第一管路311进行冲洗的具体的冲洗过程为：打开第四切换件36、第七切换件352，在清洗液或稀释液正压和抽样组件35负压动力共同作用下，清洗液或稀释液进入第一管路311，对第一管路311进行冲洗。
使用清洗液或稀释液对第一管路311进行冲洗的具体的冲洗过程为：打开第八切换件37、第七切换件352，在清洗液或稀释液正压和抽样组件35负压 动力共同作用下，清洗液或稀释液进入第二管路312，对第二管路312进行冲洗。
使用清洗液或稀释液对第三管路313进行冲洗的具体的冲洗过程为：打开第九切换39、第七切换件352，在清洗液正压和抽样组件35负压动力共同作用下，清洗液进入第三管路313，对第三管路313进行冲洗。
本发明实施例提供的样本检测方法通过使得第一待测液、第二待测液和第三待测液能够经不同的管路(分别对应第一管路311、第二管路312和第三管路313)流入检测组件32的样本针321来检测，相互之间不干扰，有利于保证检测组件32检测结果的准确度。
以上对本发明实施例进行了详细介绍，本文中应用了具体个例对本发明的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本发明的方法及其核心思想；同时，对于本领域的一般技术人员，依据本发明的思想，在具体实施方式及应用范围上均会有改变之处，综上，本说明书内容不应理解为对本发明的限制。

Claims (44)

  1. 一种样本检测装置，其特征在于，包括：
    管路组件，所述管路组件包括第一管路和第二管路；
    检测组件，所述检测组件包括样本针，所述样本针包括第一接入点；
    反应组件，所述反应组件包括第一反应池组和第二反应池组，所述第一反应池组接入所述第一管路的第二接入点上，所述第二反应池组联通至所述第二管路，所述反应组件用于对生物样本进行处理以形成待测液；
    所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述样本针的第一接入点，所述第二反应池组中的待测液经所述第二管路流动至所述样本针的第一接入点，以使得所述第一反应池组或所述第二反应池组中的待测液分别流入所述检测组件的样本针中来供检测。
  2. 如权利要求1所述的样本检测装置，其特征在于，所述样本检测装置还包括推样组件，所述推样组件分别接入所述第一管路的第三接入点和所述第二管路的第四接入点，所述第三接入点位于所述第二接入点和所述第一接入点之间，所述第三接入点与所述第一接入点之间的通道为第一待测液准备段，所述第四接入点与所述第一接入点之间的通道为第二待测液准备段；
    所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述第一管路的第三接入点，并经所述第一管路的第三接入点流入所述第一待测液准备段；所述第二反应池组中的待测液经所述第二管路流动至所述第二管路的第四接入点，并经所述第二管路的第四接入点流入所述第二待测液准备段；
    所述推样组件用于将所述第一待测液准备段或所述第二待测液准备段内的待测液推入所述检测组件的样本针中。
  3. 如权利要求2所述的样本检测装置，其特征在于，所述样本检测装置还包括抽样组件，所述抽样组件接入所述第一管路和/或所述第二管路的端部，所述抽样组件用于形成负压以将所述第一反应池组中的待测液和/或所述第二反应池组中的待测液对应抽取到所述第一待测液准备段和/或所述第二待测液准备段内。
  4. 如权利要求1所述的样本检测装置，其特征在于，所述样本检测装置还包括第一清洗组件和第二清洗组件，所述第一清洗组件接入所述第一管路，所 述第一清洗组件中的清洗液流入所述第一管路，以清洗所述第一管路；
    所述第二清洗组件接入所述第二管路，所述第二清洗组件中的清洗液流入所述第二管路，以清洗所述第二管路。
  5. 如权利要求1所述的样本检测装置，其特征在于，所述样本检测组件包括第三清洗组件，所述第一管路和所述第二管路共同连接于所述第三清洗组件上，所述第三清洁组件中的清洗液流入所述第一管路和/或所述第二管路，以清洗所述第一管路和/或所述第二管路。
  6. 如权利要求5所述的样本检测装置，其特征在于，所述第二反应池组还接入所述第一管路的第五接入点，所述第三清洗组件接入所述第一管路的端部；
    所述第三清洗组件中的清洗液自所述第一管路的端部流入所述第一管路，以清洗所述第一管路；和/或，所述第三清洗组件中的清洗液自所述第一管路的端部流至所述第一管路的第五接入点，且自所述第五接入点流入所述第二反应池组于所述第二管路的接入点，以清洗所述第二管路。
  7. 如权利要求1至6任意一项所述的样本检测装置，其特征在于，所述反应组件还包括第三反应池组，所述第三反应池组接入所述第一管路的第六接入点，所述第三反应池组中的待测液经所述第一管路的第六接入点流动至所述样本针的第一接入点，以使得所述第三反应池组中的待测液流入所述检测组件的样本针中来供检测。
  8. 如权利要求7所述的样本检测装置，其特征在于，所述样本检测装置还包括推样组件，所述推样组件分别接入所述第一管路的第三接入点和所述第二管路的第四接入点，所述第三接入点位于所述第二接入点和所述第一接入点之间，所述第三接入点与所述第一接入点之间的通道为第一待测液准备段，所述第四接入点与所述第一接入点之间的通道为第二待测液准备段；
    所述第一反应池组或所述第三反应池组中的待测液分别经所述第一管路的第二接入点或所述第一管路的第六接入点流动至所述第一管路的第三接入点，并经所述第一管路的第三接入点流入所述第一待测液准备段，和/或；所述第二反应池组中的待测液经所述第二管路流动至所述第二管路的第四接入点，并经所述第二管路的第四接入点流入所述第二待测液准备段；
    所述推样组件用于将所述第一待测液准备段或所述第二待测液准备段内 的待测液推入所述检测组件的样本针中。
  9. 如权利要求2或8所述的样本检测装置，其特征在于，所述推样组件包括注射器、设置于所述注射器的注射口的选择切换件、第四支路和第五支路，所述第四支路和所述第五支路的一端分别接入所述第三接入点和所述第四接入点，所述第四支路和所述第五支路的另一端共同设在所述选择切换件，所述选择切换件用于切换所述第四支路或所述第五支路来与所述注射器联通。
  10. 如权利要求9所述的样本检测装置，其特征在于，所述样本检测装置还包括抽样组件，所述抽样组件接入所述第一管路和/或第二管路的端部，所述抽样组件用于形成负压以将所述第一反应池组或第三反应池组中的待测液抽取到所述第一待测液准备段；和/或所述抽样组件用于形成负压以将所述第二反应池组中的待测液抽取到第二待测液准备段内。
  11. 如权利要求7所述的样本检测装置，其特征在于，所述样本检测装置还包括第四反应池组，所述第四反应池组接入所述第一管路的第十一接入点，所述第四反应池组中的待测液经所述第一管路的第十一接入点流动至所述样本针的第一接入点，以使得所述第三反应池组中的待测液流入所述检测组件的样本针中来供检测。
  12. 如权利要求1至6任意一项所述的样本检测装置，其特征在于，所述管路组件还包括第三管路，所述反应组件还包括第三反应池组，所述第三反应池组联通至所述第三管路；所述第三反应池组中的待测液经所述第三管路流动至所述样本针的第一接入点，以使得所述第三反应池组中的待测液流入所述检测组件的样本针中来供检测。
  13. 如权利要求12所述的样本检测装置，其特征在于，所述样本检测装置还包括推样组件，所述推样组件分别接入所述第一管路的第三接入点、所述第二管路的第四接入点和所述第三管路的第七接入点，所述第三接入点位于所述第二接入点和所述第一接入点之间，所述第三接入点与所述第一接入点之间的通道为第一待测液准备段，所述第四接入点与所述第一接入点之间的通道为第二待测液准备段，所述第七接入点与所述第一接入点之间的通道为第三待测液准备段；
    所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述 第一管路的第三接入点，并经所述第一管路的第三接入点流入所述第一待测液准备段；所述第二反应池组中的待测液经所述第二管路流动至所述第二管路的第四接入点，并经所述第二管路的第四接入点流入所述第二待测液准备段；所述第三反应池组中的待测液经所述第三管路流动至所述第三管路的第七接入点，并经所述第三管路的第七接入点流入所述第三待测液准备段；
    所述推样组件用于将所述第一待测液准备段、所述第二待测液准备段或所述第三待测液准备段内的待测液推入所述检测组件的样本针中。
  14. 如权利要求13所述的样本检测装置，其特征在于，所述推样组件包括注射器、设置于所述注射器的注射口的选择切换件、第四支路、第五支路和第六支路，所述第四支路、所述第五支路和所述第六支路的一端分别接入所述第三接入点、所述第四接入点和所述第七接入点，所述第四支路、所述第五支路和所述第六支路的的另一端共同设在所述选择切换件，所述选择切换件用于切换所述第四支路、所述第五支路或所述第六支路的来与所述注射器联通。
  15. 如权利要求13所述的样本检测装置，其特征在于，所述样本检测装置还包括抽样组件，所述抽样组件接入所述第一管路、所述第二管路和/或所述第三管路的端部，所述抽样组件用于形成负压以将所述第一反应池组中的待测液、所述第二反应池组中的待测液和/或所述第三反应池组中的待测液对应抽取到所述第一待测液准备段、所述第二待测液准备段和/或所述第三待测液准备段内。
  16. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第二管路接入所述第二反应池组，以使所述第二反应池组联通至所述第二管路。
  17. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第二反应池组还接入所述第一管路的第五接入点，所述第二管路接入所述第一管路的第八接入点，所述第二反应池组中的待测液自所述第五接入点流动至所述第八接入点，并自所述第八接入点流入所述第二管路，以使所述第二反应池组联通至所述第二管路；或者，
    所述第二反应池组还接入所述第一管路的第五接入点，所述第二管路接入所述第二管路的第八接入点，所述第一反应池组中的待测液自所述第二接入点流动至所述第八接入点，并自所述第八接入点流动至所述第一接入点；所述第 二管路接入所述第一管路的第八接入点，所述第二反应池组中的待测液自所述第五接入点流动至所述第八接入点，并自所述第八接入点流入所述第二管路，以使所述第二反应池组联通至所述第二管路；
    所述第二管路和所述第二反应池组共同接入所述第一管路的第五接入点，所述第二反应池组中的待测液自所述第五接入点流入所述第二管路，以使所述第二反应池组联通至所述第二管路。
  18. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第二反应池组接入所述第二管路的第九接入点，所述第二反应池组中的待测液自所述第九接入点流入所述第二管路，以使所述第二反应池组联通至所述第二管路。
  19. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第二管路接入所述第一管路上的第十接入点，所述第十接入点联通至所述样本针的第一接入点；所述第二反应池组中的待测液经所述第二管路流动至所述第一管路的第十接入点，且自所述第一管路的第十接入点流动至所述样本针的第一接入点。
  20. 如权利要求19所述的样本检测装置，其特征在于，所述第一反应池组中的待测液自所述第一管路的第二接入点流动至所述样本针的第一接入点时，不会流经所述第一管路上的第十接入点。
  21. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第二管路接入所述检测组件的第一接入点。
  22. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第一反应池组包括第一反应池、第一支路和第一切换件，所述第一反应池用于形成第一待测液，所述第一支路连接在所述第一反应池与所述第二接入点之间，所述第一切换件设在所述第一支路上，所述第一切换件用于实现第一支路的切断和联通。
  23. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第二反应池组包括第二反应池、第二支路和第二切换件，所述第二反应池用于形成第二待测液，所述第二支路的一端连接在所述第二反应池上，且所述第二支路与所述第二管路联通，所述第二切换件设在所述第二支路上，所述第二切 换件用于实现第二支路的切断或联通。
  24. 如权利要求8-15任意一项所述的样本检测装置，其特征在于，所述第三反应池组包括第三反应池、第三支路和第三切换件，所述第三反应池用于形成第三待测液，所述第三支路的一端连接在所述第三反应池上，且所述第三支路与所述第六接入点或与所述第三管路联通，所述第三切换件设在所述第三支路上，所述第三切换件用于实现第三支路的切断或联通。
  25. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述检测组件还包括流动室、鞘液池、第五切换件、第一废液池以及第六切换件，所述流动室具有待测液入口、鞘液入口以及出口，所述待测液入口连接所述样本针的第一接入点，所述鞘液入口连接所述鞘液池，所述鞘液池用于存储鞘液，所述第五切换件连接在所述鞘液池与所述鞘液入口之间，所述第五切换件用于实现切断和联通，所述出口连接所述第一废液池，所述第六切换件连接在所述第一废液池与所述出口之间，所述第六切换件用于实现切断和联通。
  26. 如权利要求3、11或15所述的样本检测装置，其特征在于，所述抽样组件包括第二废液池和第七切换件，所述第二废液池内形成负压，所述第七切换件连接在所述第二废液池与所述第一管路、所述第二管路和/或所述第三管路的端部之间，所述第七切换件用于实现切断和联通。
  27. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述样本检测装置还包括第四切换件，所述第四切换件设在所述第一管路上，所述第四切换件位于所述第三接入点与所述第二接入点之间，所述第四切换件用于实现切断或联通。
  28. 如权利要求1-15任意一项所述的样本检测装置，其特征在于，所述第八切换件设在所述第二管路上，所述第八切换件位于所述第四接入点与所述第二管路之间，所述第八切换件用于实现切断或联通。
  29. 如权利要求1-28任一项所述的样本检测装置，其特征在于，所述第一反应池组用于形成检测白细胞计数的第一待测液，所述第二反应池组用于形成检测网织红细胞计数的第二待测液，所述第三反应池组用于形成检测白细胞分类的第三待测液。
  30. 如权利要求29所述的样本检测装置，其特征在于，还包括外壳，所 述外壳包括操作端，所述第一反应池组和所述第三反应池组相对于所述第二反应池组靠近所述操作端排布。
  31. 一种样本检测方法，其特征在于，所述样本检测方法采用权利要求1所述的样本检测装置进行检测，所述样本检测方法包括：
    将第一生物样本和第二生物样本分别置于所述第一反应池组和所述第二反应池组中进行处理以形成待测液；
    使所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述样本针的第一接入点，由所述检测组件在第一时间对流入所述样本针的第一接入点的所述第一反应池组的待测液进行检测；
    使所述第二反应池组中的待测液经所述第二管路流动至所述样本针的第一接入点，由所述检测组件在第二时间对流入所述样本针的第一接入点的所述第二反应池组的待测液进行检测。
  32. 如权利要求31所述的方法，其特征在于，所述样本检测装置还包括推样组件，所述推样组件分别接入所述第一管路的第三接入点和所述第二管路的第四接入点，所述第三接入点与所述第一接入点之间的通道为第一待测液准备段，所述第四接入点与所述第一接入点之间的通道为第二待测液准备段；
    所述使所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述样本针的第一接入点包括：使所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述第一管路的第三接入点，并经所述第一管路的第三接入点流入所述第一待测液准备段，且由所述推样组件将该待测液自所述第一待测液准备段推入所述样本针的第一接入点；
    所述使所述第二反应池组中的待测液经所述第二管路流动至所述样本针的第一接入点包括：使所述第二反应池组中的待测液经所述第二管路流动至所述第二管路的第四接入点，并经所述第二管路的第四接入点流入所述第二待测液准备段，且由所述推样组件将该待测液自所述第二待测液准备段推入所述样本针的第一接入点。
  33. 如权利要求32所述的方法，其特征在于，所述样本检测装置还包括抽样组件，所述抽样组件接入所述第一管路和/或第二管路的端部；
    所述使所述第一反应池组中的待测液经所述第一管路的第二接入点流动 至所述样本针的第一接入点还包括：所述抽样组件先将所述第一反应池组中的待测液抽取到所述第一待测液准备段，所述推样组件再将该待测液自所述第一待测液准备段推入所述样本针的第一接入点；
    所述使所述第二反应池组中的待测液经所述第二管路流动至所述样本针的第一接入点包括：所述抽样组件先将所述第二反应池组中的待测液抽取到所述第二待测液准备段，所述推样组件再将该待测液自所述第二待测液准备段推入所述样本针的第一接入点。
  34. 如权利要求31-33任一项所述的方法，其特征在于，在所述检测组件对所述第一反应池组的待测液进行检测后，还包括：对所述第一管路进行清洗；和/或在所述检测组件对所述第二反应池组的待测液进行检测后，还包括：对所述第二管路进行清洗。
  35. 如权利要求31-33任一项所述的方法，其特征在于，所述样本检测装置还包括控制器，所述控制器用于统计所述检测组件分别对第一反应池组的待测液和第二反应池组的待测液进行检测的次数，并判断统计的该次数是否达到预设检测量；所述方法还包括：
    在所述检测组件对所述第一反应池组的待测液进行预设检测量的检测后，对所述第一管路进行清洗；和/或，在所述检测组件对所述第二反应池组的待测液进行预设检测量的检测后，对所述第二管路进行清洗。
  36. 如权利要求31所述的方法，其特征在于，所述反应组件还包括第三反应池组，所述第三反应池组接入所述第一管路的第六接入点，所述第三反应池组中的待测液经所述第一管路的第六接入点流动至所述样本针的第一接入点；所述样本检测方法还包括：
    将第三生物样本置于所述第三反应池组中进行处理以形成待测液；
    使所述第三反应池组中的待测液经所述第一管路的第六接入点流动至所述样本针的第一接入点，由所述检测组件在第三时间对流入所述样本针的第一接入点的所述第三反应池组的待测液进行检测。
  37. 如权利要求36所述的方法，其特征在于，所述样本检测装置还包括推样组件，所述推样组件接入所述第一管路的第三接入点，所述第三接入点与所述第一接入点之间的通道为第一待测液准备段；
    所述使所述第三反应池组中的待测液经所述第一管路的第六接入点流动至所述样本针的第一接入点包括：使所述第三反应池组中的待测液经所述第一管路的第六接入点流动至所述第一管路的第三接入点，并经所述第一管路的第三接入点流入所述第一待测液准备段，且由所述推样组件将该待测液自所述第一待测液准备段推入所述样本针的第一接入点。
  38. 如权利要求37所述的方法，其特征在于，所述样本检测装置还包括抽样组件；
    所述使所述第三反应池组中的待测液经所述第一管路的第六接入点流动至所述样本针的第一接入点还包括：所述抽样组件先通过形成负压将所述第三反应池组中的待测液抽取到所述第一待测液准备段，所述推样组件再将该待测液自所述第一待测液准备段推入所述样本针的第一接入点。
  39. 如权利要求31所述的方法，其特征在于，所述管路组件还包括第三管路，所述反应组件还包括第三反应池组，所述第三反应池组联通至所述第三管路；所述第三反应池组中的待测液经所述第三管路流动至所述样本针的第一接入点；所述样本检测方法还包括：
    将第三生物样本置于所述第三反应池组中进行处理以形成待测液；
    使所述第三反应池组中的待测液经所述第三管路流动至所述样本针的第一接入点，由所述检测组件在第三时间段对流入所述样本针的第一接入点的所述第三反应池组的待测液进行检测。
  40. 如权利要求39所述的方法，其特征在于，所述样本检测装置还包括推样组件，所述推样组件接入所述第三管路的第七接入点，所述第七接入点与所述第一接入点之间的通道为第三待测液准备段；
    所述使所述第三反应池组中的待测液经所述第三管路流动至所述样本针的第一接入点包括：使所述第三反应池组中的待测液经所述第三管路流动至所述第三管路的第七接入点，并经所述第三管路的第七接入点流入所述第三待测液准备段，且由所述推样组件将该待测液自所述第三待测液准备段推入所述样本针的第一接入点。
  41. 如权利要求40所述的方法，其特征在于，所述样本检测装置还包括抽样组件；
    所述使所述第三反应池组中的待测液经所述第三管路流动至所述样本针的第一接入点还包括：所述抽样组件先通过形成负压将所述第三反应池组中的待测液抽取到所述第三待测液准备段内，所述推样组件再将该待测液自所述第三待测液准备段推入所述样本针的第一接入点。
  42. 如权利要求31所述的方法，其特征在于，所述第二管路接入所述第一管路上的第十接入点，所述第十接入点联通至所述样本针的第一接入点；
    所述使所述第二反应池组中的待测液经所述第二管路流动至所述样本针的第一接入点包括：使所述第二反应池组中的待测液先经所述第二管路流动至所述第一管路上的第十接入点，再自所述第一管路的第十接入点流动至所述样本针的第一接入点。
  43. 如权利要求42所述的方法，其特征在于，所述使所述第一反应池组中的待测液经所述第一管路的第二接入点流动至所述样本针的第一接入点包括：使所述第一反应池组中的待测液不流经所述第一管路上的第十接入点，自所述第一管路的第二接入点流动至所述样本针的第一接入点。
  44. 一种样本分析仪，其特征在于，包括采样组件和如权利要求1至30任意一项所述的样本检测装置，所述采样组件包括采样器，所述采样器采集生物样本，且移动至所述反应组件的上方，以将所述生物样本分配至所述反应组件中。
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