WO2019038555A3 - Système d'expression de tat - Google Patents

Système d'expression de tat Download PDF

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Publication number
WO2019038555A3
WO2019038555A3 PCT/GB2018/052403 GB2018052403W WO2019038555A3 WO 2019038555 A3 WO2019038555 A3 WO 2019038555A3 GB 2018052403 W GB2018052403 W GB 2018052403W WO 2019038555 A3 WO2019038555 A3 WO 2019038555A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression system
tat
cell
present
tat expression
Prior art date
Application number
PCT/GB2018/052403
Other languages
English (en)
Other versions
WO2019038555A2 (fr
Inventor
Douglas Browning
Colin Robinson
Original Assignee
The University Of Birmingham
The University Of Kent
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Birmingham, The University Of Kent filed Critical The University Of Birmingham
Publication of WO2019038555A2 publication Critical patent/WO2019038555A2/fr
Publication of WO2019038555A3 publication Critical patent/WO2019038555A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un système d'expression pour la production de protéines recombinantes, en particulier un système d'expression de translocase à arginine double (Tat). La présente invention concerne une cellule qui est modifiée au moyen du système d'expression de Tat, des cultures comprenant la cellule, et un procédé de production de polypeptides recombinants à l'aide de la cellule. L'invention concerne également des peptides signal améliorés pour la sécrétion de protéines recombinantes par l'intermédiaire de la voie Tat.
PCT/GB2018/052403 2017-08-25 2018-08-23 Système d'expression de tat WO2019038555A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1713732.4 2017-08-25
GBGB1713732.4A GB201713732D0 (en) 2017-08-25 2017-08-25 Tat expression system

Publications (2)

Publication Number Publication Date
WO2019038555A2 WO2019038555A2 (fr) 2019-02-28
WO2019038555A3 true WO2019038555A3 (fr) 2019-04-04

Family

ID=60037327

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2018/052403 WO2019038555A2 (fr) 2017-08-25 2018-08-23 Système d'expression de tat

Country Status (2)

Country Link
GB (1) GB201713732D0 (fr)
WO (1) WO2019038555A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3069197A1 (fr) * 2017-07-07 2019-01-10 Allena Pharmaceuticals, Inc. Enzyme uricase recombinante

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022667A2 (fr) * 2000-09-18 2002-03-21 Genencor International, Inc. Translocation des arginines couplees dans bacillus
WO2003040335A2 (fr) * 2001-11-05 2003-05-15 Research Development Foundation Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries
US20140154742A1 (en) * 2011-04-08 2014-06-05 Anthem Biosciences Pvt Ltd. Novel expression and secretion vector systems for heterologous protein production in escherichia coli

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022667A2 (fr) * 2000-09-18 2002-03-21 Genencor International, Inc. Translocation des arginines couplees dans bacillus
WO2003040335A2 (fr) * 2001-11-05 2003-05-15 Research Development Foundation Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries
US20140154742A1 (en) * 2011-04-08 2014-06-05 Anthem Biosciences Pvt Ltd. Novel expression and secretion vector systems for heterologous protein production in escherichia coli

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ANTHONY J. SNYDER ET AL: "ABSTRACT", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 80, no. 10, 15 May 2014 (2014-05-15), US, pages 3219 - 3232, XP055527828, ISSN: 0099-2240, DOI: 10.1128/AEM.00335-14 *
BEN C. BERKS: "The Twin-Arginine Protein Translocation Pathway", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 84, no. 1, 2 June 2015 (2015-06-02), US, pages 843 - 864, XP055527808, ISSN: 0066-4154, DOI: 10.1146/annurev-biochem-060614-034251 *
CRISTINA F.R.O MATOS ET AL: "High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli", BIOTECHNOLOGY AND BIOENGINEERING, 1 October 2012 (2012-10-01), Hoboken, pages 2533 - 2542, XP055527822, Retrieved from the Internet <URL:https://pdfs.semanticscholar.org/a8c0/1faeda10742d8a1ef011566aa02699a5fd53.pdf> DOI: 10.1002/bit.24535 *
DOUGLAS F. BROWNING ET AL: "Escherichia coli "TatExpress" strains super-secrete human growth hormone into the bacterial periplasm by the Tat pathway", BIOTECHNOLOGY AND BIOENGINEERING, vol. 114, no. 12, 6 October 2017 (2017-10-06), US, pages 2828 - 2836, XP055527816, ISSN: 0006-3592, DOI: 10.1002/bit.26434 *
RIBNICKY ET AL: "A scFv Antibody Mutant Isolated in a Genetic Screen for Improved Export via the Twin Arginine Transporter Pathway Exhibits Faster Folding", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 369, no. 3, 11 May 2007 (2007-05-11), pages 631 - 639, XP022077558, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2007.03.068 *
STEVEN D. BRANSTON ET AL: "Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery", BIOTECHNOLOGY AND BIOENGINEERING, vol. 109, no. 4, 1 April 2012 (2012-04-01), US, pages 983 - 991, XP055528344, ISSN: 0006-3592, DOI: 10.1002/bit.24384 *
XIONG ET AL: "Expression level of heterologous tat genes is crucial for in vivo reconstitution of a functional Tat translocase in Escherichia coli", BIOCHIMIE, MASSON, PARIS, FR, vol. 89, no. 5, 3 May 2007 (2007-05-03), pages 676 - 685, XP022081073, ISSN: 0300-9084, DOI: 10.1016/J.BIOCHI.2007.01.006 *

Also Published As

Publication number Publication date
WO2019038555A2 (fr) 2019-02-28
GB201713732D0 (en) 2017-10-11

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