WO2019023961A1 - Procédé de capture de cellules ou de molécules cibles en solution - Google Patents

Procédé de capture de cellules ou de molécules cibles en solution Download PDF

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Publication number
WO2019023961A1
WO2019023961A1 PCT/CN2017/095558 CN2017095558W WO2019023961A1 WO 2019023961 A1 WO2019023961 A1 WO 2019023961A1 CN 2017095558 W CN2017095558 W CN 2017095558W WO 2019023961 A1 WO2019023961 A1 WO 2019023961A1
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WO
WIPO (PCT)
Prior art keywords
molecules
cells
capturing device
target cells
capturing
Prior art date
Application number
PCT/CN2017/095558
Other languages
English (en)
Inventor
Jing YAN
Jerome Charmet
Bauer Wolfgang-Andreas CHRISTIAN
Ziyi Yu
Yang Yang
Original Assignee
Suzhou Bofu Biomedical Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Bofu Biomedical Limited filed Critical Suzhou Bofu Biomedical Limited
Priority to EP17920323.7A priority Critical patent/EP3662059A4/fr
Priority to PCT/CN2017/095558 priority patent/WO2019023961A1/fr
Priority to US16/625,658 priority patent/US11525829B2/en
Publication of WO2019023961A1 publication Critical patent/WO2019023961A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N15/0606—Investigating concentration of particle suspensions by collecting particles on a support
    • G01N15/0618—Investigating concentration of particle suspensions by collecting particles on a support of the filter type
    • G01N15/0631—Separation of liquids, e.g. by absorption, wicking
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé de capture de cellules ou de molécules cibles en solution, comprenant les étapes consistant à : (I) introduire un milieu contenant lesdites cellules ou molécules cibles dans un appareil comprenant un dispositif de capture pour capturer lesdites cellules ou molécules cibles ; (II) amener ledit milieu à s'écouler à travers ledit dispositif de capture ; (III) éliminer les débris, les cellules et les molécules non liés ; (IV) amener lesdites cellules ou molécules cibles à se détacher du dispositif de capture ; et (V) collecter lesdites cellules ou molécules cibles ; ledit dispositif de capture comprenant au moins un maillage fonctionnalisé comprenant un substrat maillé et une couche fonctionnelle formée sur ledit substrat maillé, ladite couche fonctionnelle comprenant des substances de capture qui peuvent se lier de manière spécifique auxdites cellules ou molécules cibles. Le procédé présente une spécificité élevée, ainsi qu'un rendement élevé, et est approprié pour capturer des cellules ou des molécules dans une solution ou exprimées à la surface de membranes cellulaires. Il est particulièrement approprié pour capturer et trier des cellules tumorales circulantes.
PCT/CN2017/095558 2017-08-02 2017-08-02 Procédé de capture de cellules ou de molécules cibles en solution WO2019023961A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP17920323.7A EP3662059A4 (fr) 2017-08-02 2017-08-02 Procédé de capture de cellules ou de molécules cibles en solution
PCT/CN2017/095558 WO2019023961A1 (fr) 2017-08-02 2017-08-02 Procédé de capture de cellules ou de molécules cibles en solution
US16/625,658 US11525829B2 (en) 2017-08-02 2017-08-02 Method for capturing target cells or molecules in solution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/095558 WO2019023961A1 (fr) 2017-08-02 2017-08-02 Procédé de capture de cellules ou de molécules cibles en solution

Publications (1)

Publication Number Publication Date
WO2019023961A1 true WO2019023961A1 (fr) 2019-02-07

Family

ID=65232174

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2017/095558 WO2019023961A1 (fr) 2017-08-02 2017-08-02 Procédé de capture de cellules ou de molécules cibles en solution

Country Status (3)

Country Link
US (1) US11525829B2 (fr)
EP (1) EP3662059A4 (fr)
WO (1) WO2019023961A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112251393B (zh) * 2020-10-19 2022-05-10 北京龙迈达斯科技开发有限公司 一种细胞分离装置

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010108003A2 (fr) * 2009-03-18 2010-09-23 The Regents Of The University Of California Dispositif de capture de cellules circulantes
US7855068B2 (en) * 2002-04-25 2010-12-21 Semibio Holdings Limited Methods and kits for detecting a target cell
WO2012051390A1 (fr) * 2010-10-14 2012-04-19 Veridex, Llc Procédés et trousses pour la détection de cellules tumorales circulantes chez des patients pancréatiques à l'aide de réactifs polyspécifiques de capture et de détection de cocktail
CN102978105A (zh) * 2012-12-24 2013-03-20 天津启仁医药科技有限公司 一种mRNA快速提取试剂盒
WO2014153262A1 (fr) * 2013-03-14 2014-09-25 Sevident, Inc. Filets moléculaires sur phases solides
CN105504301A (zh) * 2015-12-07 2016-04-20 复旦大学 一种树枝大分子—共聚物细胞捕获材料及其制备方法和应用
CN205501299U (zh) * 2016-02-19 2016-08-24 德路通（石家庄）生物科技有限公司 捕获单元及分离器
CN205501298U (zh) * 2016-02-19 2016-08-24 德路通（石家庄）生物科技有限公司 过滤系统及循环滤除系统
CN106635769A (zh) * 2016-11-07 2017-05-10 深圳市达科为生物工程有限公司 细胞分离装置以及细胞分离方法
CN107338184A (zh) * 2017-08-02 2017-11-10 苏州博福生物医药科技有限公司 一种用于捕获细胞或溶液中生物分子的捕获筛及装置
CN107338185A (zh) * 2017-08-02 2017-11-10 苏州博福生物医药科技有限公司 一种细胞或溶液中生物分子的捕获方法

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DE10122659A1 (de) * 2001-05-10 2002-12-05 Infineon Technologies Ag Biochip-Anordnung
AU2004286307A1 (en) * 2003-10-31 2005-05-12 Vitatex, Inc. Blood test prototypes and methods for the detection of circulating tumor and endothelial cells
WO2008131048A2 (fr) * 2007-04-16 2008-10-30 Cellpoint Diagnotics, Inc. Dispositifs et procédés permettant de diagnostiquer, de pronostiquer ou de théranoser un état pathologique par enrichissement de cellules rares
US20140154703A1 (en) * 2011-01-06 2014-06-05 Alison Skelley Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size
JP2016527494A (ja) * 2013-07-05 2016-09-08 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャライゼーション マイクロ流体アッセイのための方法、組成物およびシステム
CN105861297A (zh) * 2016-03-29 2016-08-17 厦门大学 一种循环肿瘤细胞检测芯片及其应用
US20200156073A1 (en) * 2017-08-02 2020-05-21 Hemosmart Medical Technology Ltd. Functionalized mesh and fluidic apparatus for capturing cells or molecules in solution
CN108753569A (zh) * 2018-05-25 2018-11-06 苏州博福生物医药科技有限公司 一种用于捕获细胞或生物分子的富集筛选机构

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7855068B2 (en) * 2002-04-25 2010-12-21 Semibio Holdings Limited Methods and kits for detecting a target cell
WO2010108003A2 (fr) * 2009-03-18 2010-09-23 The Regents Of The University Of California Dispositif de capture de cellules circulantes
WO2012051390A1 (fr) * 2010-10-14 2012-04-19 Veridex, Llc Procédés et trousses pour la détection de cellules tumorales circulantes chez des patients pancréatiques à l'aide de réactifs polyspécifiques de capture et de détection de cocktail
CN102978105A (zh) * 2012-12-24 2013-03-20 天津启仁医药科技有限公司 一种mRNA快速提取试剂盒
WO2014153262A1 (fr) * 2013-03-14 2014-09-25 Sevident, Inc. Filets moléculaires sur phases solides
CN105504301A (zh) * 2015-12-07 2016-04-20 复旦大学 一种树枝大分子—共聚物细胞捕获材料及其制备方法和应用
CN205501299U (zh) * 2016-02-19 2016-08-24 德路通（石家庄）生物科技有限公司 捕获单元及分离器
CN205501298U (zh) * 2016-02-19 2016-08-24 德路通（石家庄）生物科技有限公司 过滤系统及循环滤除系统
CN106635769A (zh) * 2016-11-07 2017-05-10 深圳市达科为生物工程有限公司 细胞分离装置以及细胞分离方法
CN107338184A (zh) * 2017-08-02 2017-11-10 苏州博福生物医药科技有限公司 一种用于捕获细胞或溶液中生物分子的捕获筛及装置
CN107338185A (zh) * 2017-08-02 2017-11-10 苏州博福生物医药科技有限公司 一种细胞或溶液中生物分子的捕获方法

Non-Patent Citations (1)

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Title
See also references of EP3662059A4 *

Also Published As

Publication number Publication date
EP3662059A4 (fr) 2021-03-24
EP3662059A1 (fr) 2020-06-10
US20200150114A1 (en) 2020-05-14
US11525829B2 (en) 2022-12-13

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