WO2019010788A1 - Puce de prétraitement de séparation du sang et dispositif de séparation du sang - Google Patents

Puce de prétraitement de séparation du sang et dispositif de séparation du sang Download PDF

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Publication number
WO2019010788A1
WO2019010788A1 PCT/CN2017/100741 CN2017100741W WO2019010788A1 WO 2019010788 A1 WO2019010788 A1 WO 2019010788A1 CN 2017100741 W CN2017100741 W CN 2017100741W WO 2019010788 A1 WO2019010788 A1 WO 2019010788A1
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Prior art keywords
blood
array
microcolumn
cells
diameter
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PCT/CN2017/100741
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English (en)
Chinese (zh)
Inventor
韩琳
丁庆
刘荣跃
杨彬
李辰
Original Assignee
华讯方舟科技有限公司
深圳市太赫兹科技创新研究院有限公司
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Publication of WO2019010788A1 publication Critical patent/WO2019010788A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped

Definitions

  • the embodiments of the present invention belong to the field of biomedical technology, and in particular, to a blood separation pretreatment chip and a blood separation device.
  • the therapeutic effect can be through various markers in the blood.
  • the results of the reaction are measured.
  • the blood sample of a cancer patient is detected by a blood tester, which can realize the non-injury test of the cancer patient, monitor the patient's condition, and provide an important basis for the doctor and the doctor to adjust the reasonable treatment plan for accurate medical treatment. Testing provides a solid foundation.
  • biomolecules other than proteins, miRNAs and the like in the blood also contain a large number of cells of different sizes.
  • serum needs to be separated from the blood.
  • the traditional method of serum separation is to freeze the blood sample for a period of time, and then separate the blood sample by a centrifuge. This method of serum separation requires a large amount of blood and is prolonged, which seriously reduces the detection efficiency of blood samples.
  • Embodiments of the present invention provide a blood separation pretreatment chip and a blood separation device, which are capable of rapidly separating a very small amount of blood samples and improving the detection efficiency of blood samples.
  • An embodiment of the present invention provides a blood separation pretreatment chip including at least one microcolumn array sequentially arranged along a blood flow direction, each of the microcolumn arrays having different column spacings, the at least one The column spacing of the microcolumn array is sequentially decreased according to the arrangement order of the at least one microcolumn array, and the at least one microcolumn array includes at least an array of red blood cell trapping micropillars;
  • the red blood cell trapping microcolumn array intercepts red blood cells in the blood to filter red blood cells in the blood; [0007] blood flows in from the inlet of the blood separation pretreatment chip, and is sequentially intercepted and filtered by the at least one microcolumn array to obtain plasma containing less than a predetermined amount of red blood cells and platelets from the blood separation pretreatment The exit of the chip flows out.
  • the at least one microcolumn array comprises a tumor cell trapping microcolumn array, a monocyte trapping microcolumn array, a leukocyte truncated microcolumn array, and the red blood cell trapping microcolumn arranged in sequence along the blood flow direction.
  • the tumor cell entrapment microcolumn array intercepts tumor cells in the blood to filter tumor cells in the blood; the monocyte traps microcolumn arrays against monocytes in the blood Performing entrapment to filter monocytes in the blood; the leukocyte-retaining microcolumn array intercepts leukocytes in the blood to filter leukocytes in the blood;
  • the blood flows in from the inlet of the blood separation pretreatment chip, sequentially passes through the tumor cell intercepting microcolumn array, the monocyte trapping microcolumn array, the leukocyte-retained microcolumn array, and the red blood cell
  • the microcolumn array is trapped, and tumor cells, monocytes, white blood cells, and red blood cells in the blood are intercepted and filtered, and plasma containing less than a predetermined amount of red blood cells and platelets is obtained from the outlet of the blood separation pretreatment chip.
  • the array of micropillars comprises a plurality of rows of microcolumns arranged in sequence along the direction of blood flow, and any two adjacent rows of the microcolumns are misaligned.
  • the column spacing of the tumor cell-retaining microcolumn array is less than or equal to the diameter of the tumor cells and greater than the diameter of monocytes, white blood cells, red blood cells, and platelets in the blood.
  • the column spacing of the monocyte trapped microcolumn array is less than or equal to the diameter of the monocyte and greater than the diameter of leukocytes, red blood cells, and platelets in the blood.
  • the column spacing of the leukocyte-retaining microcolumn array is less than or equal to the diameter of the leukocytes and greater than the diameter of red blood cells and platelets in the blood.
  • the column spacing of the array of red blood cell trapped micropillars is less than or equal to the diameter of the red blood cells and greater than the diameter of the platelets in the blood.
  • the array of micropillars is any one of a cylindrical array, an elliptical cylinder array, or a polygonal cylinder array.
  • Another aspect of an embodiment of the present invention further provides a blood separation device comprising the above-mentioned blood separation pre-treatment
  • the microchip includes a microfluidic chip, and the microfluidic chip is provided with a microchannel connected to an outlet of the blood separation pretreatment chip;
  • the micro flow channel includes a plurality of micro flow channel units arranged in a periodic manner, and the plurality of micro flow channel units are connected end to end in sequence;
  • the micro flow channel unit includes a first semi-annular micro flow channel and a second semi-annular micro flow channel, and the inlets of the first semi-annular micro flow channel outlet and the second semi-annular micro flow channel are seamless Docking;
  • the micro flow channel performs inertial focusing treatment on the blood after the pretreatment , to obtain high purity plasma.
  • a difference between an outer diameter and an inner diameter of the first semi-annular microchannel is equal to a circumscribed diameter at any position on the first semi-annular microchannel, the second semicircular micro
  • the difference between the outer diameter and the inner diameter of the flow passage is smaller than the maximum circular cut diameter of the second semi-annular micro flow passage, and the outer diameter of the first semi-annular micro flow passage is smaller than the inner diameter of the second semi-annular micro flow passage.
  • At least one microcolumn array including an array of red blood cell trapping micropillars for trapping and filtering red blood cells is sequentially disposed along the blood flow direction, and at least one column spacing of each microcolumn array is different, so that at least one The column spacing of the microcolumn array is sequentially decreased in accordance with the arrangement order of the at least one microcolumn array, so that blood flows in from the inlet of the blood separation pretreatment chip, and sequentially passes through the interception and filtration of the at least one microcolumn array to obtain a low content.
  • the plasma of the preset amount of red blood cells and platelets flows out from the outlet of the blood separation pretreatment chip, so that a rapid separation of a very small amount of blood sample can be achieved, and the detection efficiency of the blood sample can be improved.
  • FIG. 1 is a schematic perspective view of a blood separation pretreatment chip according to an embodiment of the present invention.
  • FIG. 2 is a plan view of a blood separation pretreatment chip according to an embodiment of the present invention.
  • FIG. 3 is a schematic structural view of a blood separation device according to an embodiment of the present invention.
  • FIG. 4 is a front elevational view of a micro flow channel chip according to an embodiment of the present invention.
  • an embodiment of the present invention provides a blood separation pretreatment chip 10, which comprises four microcolumn arrays arranged in sequence along a blood flow direction, and four microcolumn arrays respectively contain tumor cell retention microscopy.
  • Column array 1 mononuclear cell retention microcolumn array 2, white blood cell retention microcolumn array 3, and red blood cell retention microcolumn array 4; the column spacing of each microcolumn array is different, and the column spacing of the four microcolumn arrays is microscopic
  • the order of arrangement of the column arrays is sequentially decreased, that is, the column spacing of the tumor cell intercepting microcolumn array 1 > the column spacing of the mononuclear cell retention microcolumn array IJ2 > the column spacing of the white blood cell microcolumn array IJ3 > the red blood cell intercepting microcolumn array ⁇ IJ4 column spacing.
  • the column spacing specifically refers to the width of the gap between any adjacent two microcolumns in the direction perpendicular to the blood flow direction in any microcolumn array, the column in this embodiment
  • the spacing specifically refers to the lateral column spacing.
  • any adjacent two of the microcolumn arrays in a direction parallel to the flow of blood can be set according to actual needs, and the longitudinal column spacing can be equal to the lateral column spacing.
  • the micropillar array can be any of a cylindrical array, an elliptical cylinder array, or a polygonal cylinder array. Only the case where the micropillar array is a cylindrical array is exemplarily shown in Fig. 1.
  • the working principle of the blood separation pretreatment chip provided in this embodiment is:
  • the tumor cell entrapment microcolumn array intercepts tumor cells in the blood to filter tumor cells in the blood;
  • the monocyte trap microcolumn array intercepts monocytes in the blood to filter mononuclear blood The cells;
  • the leukocyte-retaining microcolumn array intercepts white blood cells in the blood to filter white blood cells in the blood;
  • the red blood cell intercepting microcolumn array intercepts red blood cells in the blood to filter red blood cells in the blood;
  • the blood separation pretreatment chip includes at least one microcolumn array and the at least one microcolumn array includes at least an array of red blood cell trapping microcolumns for trapping and filtering red blood cells, and blood is separated from the blood.
  • the inlet flow of the processing chip is sequentially intercepted and filtered by at least one microcolumn array to obtain plasma containing less than a predetermined amount of red blood cells and platelets from the outlet of the blood separation pretreatment chip.
  • setting the blood separation pretreatment chip includes at least an array of red blood cell trapping microcolumns, which can intercept and filter all cells in the blood to obtain a content lower than a preset amount. Plasma of red blood cells and platelets.
  • the filtered plasma also contains less than a predetermined amount of red blood cells because the red blood cells are flat, and the column spacing cannot be smaller than the size of all red blood cells due to the limitation of the manufacturing process. Therefore, some red blood cells cannot be effectively trapped.
  • the amount of the preset amount can be determined by the number of microcolumn arrays and the column spacing. The smaller the column spacing, the smaller the preset amount.
  • the number and type of microcolumn arrays included in the blood separation pretreatment chip can be set according to actual needs. For example, if you only need to intercept and filter tumor cells, you can only set the tumor size. The cell is trapped in the microcolumn array; only the monocyte trapping microcolumn array can be set only by intercepting and filtering the monocytes. The same type of micro-column array can also be set at the same time to enhance the interception filtering effect.
  • the present invention provides at least one microcolumn by sequentially arranging at least one microcolumn array including an array of red blood cell trapped micropillars for trapping and filtering red blood cells along the blood flow direction, and making the column spacing of each microcolumn array different
  • the column spacing of the array is sequentially decreased in accordance with the arrangement order of the at least one microcolumn array, so that blood flows in from the inlet of the blood separation pretreatment chip, sequentially passes through the interception and filtration of the at least one microcolumn array, and the content is lower than the pre-prepared
  • the amount of red blood cells and platelets of plasma flow out from the outlet of the blood separation pretreatment chip, which can achieve rapid separation of a very small amount of blood samples and improve the detection efficiency of blood samples.
  • this embodiment exemplarily shows the size structure of each microcolumn in the blood separation pretreatment chip 10.
  • the four microcolumn arrays each include a plurality of rows of microcolumns arranged in sequence along the blood flow direction, and any two adjacent rows of microcolumns are misaligned.
  • the misalignment arrangement specifically means that the adjacent two rows of microcolumns are not disposed opposite each other, but are arranged at a certain distance from each other, so that the entire microcolumn array forms an oblique array, obliquely
  • the degree of tilt of the array can be set according to actual needs.
  • each row of microcolumns in a microcolumn array can also be positioned facing up.
  • the blood cells can easily flow out along the unlineed straight line gap, thereby reducing the interception and filtering effect on blood cells.
  • each microcolumn array in order to achieve a better hematofiltration effect, it is desirable to have a column spacing of each microcolumn array that is smaller than the diameter of the blood cells that it needs to intercept and filter.
  • the column spacing of the tumor cell-retaining microcolumn array is less than or equal to the diameter of the tumor cells and greater than the diameter of monocytes, white blood cells, red blood cells, and platelets in the blood.
  • the diameter of the tumor cells is usually 17 ⁇ m to 52 ⁇ m, and therefore, the column spacing of the tumor cell-carrying microcolumn array should be less than or equal to 17 ⁇ m or slightly larger than 17 ⁇ m to achieve entrapment of most tumor cells.
  • the column spacing of the tumor cell trapped microcolumn array can range from 17 ⁇ to 25 ⁇ .
  • the column spacing of the tumor cell-retaining microcolumn array 1 is 20 ⁇ m, and the diameter of the tumor cell-retaining micro-column is 20 ⁇ m.
  • the cross-sectional size and height of each microcolumn in the tumor cell-retaining microcolumn array may be set according to actual needs, for example, the height may be greater than 52 ⁇ m ; when the tumor cells intercept the microcolumn width in the microcolumn array The range is 10 ⁇ 30 ⁇ , for example, 10 ⁇ , 15 ⁇ , 20 ⁇ , 25 ⁇ or 30 ⁇ ; when the microcolumn is a cylindrical crucible, the microcolumn width refers to the diameter of the cylinder; when the microcolumn is a square cylinder, the microcolumn width refers to the square column Square side length.
  • the column spacing of the monocyte trapped microcolumn array is less than or equal to the diameter of the monocytes and greater than the diameter of the white blood cells, red blood cells, and platelets in the blood.
  • the diameter of the monocytes is usually 15 ⁇ m to 25 ⁇ m, and therefore, the column spacing of the mononuclear-trapped microcolumn array should be less than or equal to 15 ⁇ m or slightly more than 15 ⁇ m to achieve a majority of monocytes. Interception.
  • the column spacing of the mononuclear-retained microcolumn array can range from 15 ⁇ to 17 ⁇ .
  • the column spacing of the mononuclear cell retention microcolumn array 1 is 15 ⁇ m, and the diameter of the mononuclear cell retention microcolumn is 18 ⁇ m.
  • the cross-sectional size and height of each microcolumn in the mononuclear cell retention microcolumn array can be set according to actual needs, for example, the height can be greater than 25 ⁇ m ; when the mononuclear cells are trapped in the microcolumn array
  • the width of the column ranges from 10 ⁇ to 30 ⁇ , for example, 10 ⁇ , 15 ⁇ , 20 ⁇ , 25 ⁇ or 30 ⁇ ; when the microcolumn is a cylindrical crucible, the microcolumn width refers to the diameter of the cylinder; when the microcolumn is a square cylinder, the microcolumn width refers to The square side of the square column is long.
  • the column spacing of the leukocyte-retaining microcolumn array is less than or equal to the diameter of the leukocytes and greater than the diameter of the red blood cells and platelets in the blood.
  • the diameter of the white blood cells is usually 7 ⁇ 10 ⁇ , 12 ⁇ 20 ⁇ or 14 ⁇ 20 ⁇
  • the column spacing of the leukocyte-retained microcolumn array should be less than or equal to 7 ⁇ or slightly greater than 7 ⁇ to achieve retention of most white blood cells.
  • the column spacing of a white blood cell trapped microcolumn array can be 7 ⁇ 1
  • the column spacing of the leukocyte-retaining micro-column array ij l is 10 ⁇ m, and the diameter of the leukocyte-retaining micro-column is 12 ⁇ m.
  • the cross-sectional size and height of each microcolumn in the leukocyte-retaining micro-column array can be set according to actual needs, for example, the height can be greater than 20 ⁇ m ;
  • the micro-column width in the white blood cell-carrying micro-column array is 5 ⁇ ⁇ 25 ⁇ , for example, 5 ⁇ , 10 ⁇ , 15 ⁇ , 20 ⁇ or 25 ⁇ ; when the microcolumn is a cylindrical crucible, The microcolumn width refers to the diameter of the cylinder; when the microcolumn is a square column, the microcolumn width refers to the square side length of the square column.
  • the column spacing of the array of red blood cell trapped micropillars is less than or equal to the diameter of the red blood cells and greater than the diameter of the platelets in the blood.
  • the diameter of the red blood cells is usually 6 ⁇ to 8 ⁇ . Therefore, the column spacing of the red blood cell trapping microcolumn array should be less than or equal to 6 ⁇ or slightly larger than 6 ⁇ to achieve entrapment of most red blood cells.
  • the column spacing of the erythrocyte-intercepting microcolumn array can range from 4 ⁇ to 7 ⁇ .
  • the column spacing of the red blood cell trapping microcolumn ij ij l is 5 ⁇ m, and the diameter of the red blood cell trapping microcolumn is 10 ⁇ m.
  • the cross-sectional size and height of each microcolumn in the red blood cell trapping microcolumn array can be set according to actual needs, for example, the height can be greater than 8 ⁇ m ;
  • the microcolumn width in the red blood cell trapping microcolumn array is 5 ⁇ ⁇ 25 ⁇ , for example, 5 ⁇ , 10 ⁇ , 15 ⁇ , 20 ⁇ or 25 ⁇ ;
  • the microcolumn width refers to the diameter of the cylinder; when the microcolumn is a square cylinder, the microcolumn width refers to the square of the square column Side length.
  • an embodiment of the present invention further provides a blood separation device including the blood separation pretreatment chip 10, further comprising a microfluidic chip 20, and the microfluidic chip 20 is disposed on the microfluidic chip 20 A microchannel 21 connected to the outlet of the blood separation pretreatment chip 10 and a plasma outlet and a blood cell outlet connected to the outlet of the microchannel.
  • the direction of the solid arrow in FIG. 3 indicates the direction of the blood main flow.
  • the micro flow path 21 includes a plurality of micro flow path units 211 (the portion where the dotted circles are shown in FIG. 3 is a micro flow path unit), and the plurality of micro flow path units 211 are connected end to end in sequence.
  • the microchannel unit 211 includes a first semi-annular microchannel and a second semi-annular microchannel, and the inlets of the first annular microchannel outlet and the second semicircular microchannel are seamlessly docked.
  • the size of the micro flow channel can be set according to actual needs.
  • the difference between the outer diameter and the inner diameter of the first semi-annular microchannel is equal to the circumscribed diameter at any point on the first semi-annular microchannel, and the outer diameter of the second semi-circular microchannel is The difference between the inner diameters is smaller than the maximum circumscribed diameter of the second semicircular microchannel, and the outer diameter of the first semicircular microchannel is smaller than the inner diameter of the second semicircular microchannel
  • the working principle of the blood separation device is as follows: [0069] After the blood is passed through the blood separation pretreatment chip and pretreated, the microfluidic chip enters the microchannel through the inlet of the microfluidic chip, and the microchannel performs inertial focusing treatment on the pretreated blood to obtain high purity plasma and blood cells. High-purity plasma flows out through the plasma outlet, and blood cells flow out through the blood cell outlet.
  • the inertial focusing process specifically refers to: filtering the residual small-sized cells or particles in the plasma by inertial focusing, and the particles such as cells flow in the micro-flow channel, in addition to being driven by the mainstream driving force,
  • the shear force caused by the difference in velocity gradient of the fluid and the Wall Effect Lift Force caused by the closed channel wall are combined, and the shear force and the wall lift are combined into an inertial force.
  • the cells Under the action of inertial force, the cells will migrate at a fixed position in the microchannel, so they can be used to separate platelets and a small amount of red blood cells in plasma to obtain high-purity plasma.
  • FIG. 4 a specific size structure of the micro flow path 21 is exemplarily shown in the present embodiment.
  • a specific size structure of the micro flow path 21 is exemplarily shown in the present embodiment.
  • only two cycles of the micro flow channel unit are shown by way of example.
  • the difference between the outer diameter R3 and the inner diameter R4 of the second semicircular microchannel is smaller than the maximum circumscribed diameter L2 of the second semicircular microchannel, that is, R3-R4 ⁇ L2;
  • the outer diameter R1 of the first annular microchannel is smaller than the first
  • the circumscribed diameter L1 at any position on the first semicircular microchannel is smaller than the maximum secant diameter L2 of the second semicircular microchannel, that is, LI ⁇ L2

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Abstract

L'invention concerne une puce de prétraitement de séparation du sang et un dispositif de séparation du sang. La puce de prétraitement de séparation du sang comprend au moins un réseau de micropiliers disposés l'un après l'autre dans le sens du flux sanguin, les espacements des piliers dans chacun de ces réseaux de micropiliers étant différents les uns des autres, les espacements des piliers d'au moins un des réseaux de micropiliers mentionnés diminuent successivement en fonction de la séquence d'agencement dudit réseau de micropiliers, et au moins un de ces réseaux de micropiliers comprend au moins un réseau de micropiliers de piégeage de globules rouges. Ledit réseau de micropiliers de piégeage de globules rouges piège les globules rouges dans le sang, de façon à filtrer les globules rouges dans le sang. Le sang s'écoule de l'entrée de ladite puce de prétraitement de séparation du sang et est soumis à un piégeage et à une filtration au moyen dudit réseau de micropiliers en séquence, de manière à obtenir un plasma comprenant moins d'une quantité prédéterminée de globules rouges et de plaquettes, et est évacué par la sortie de ladite puce de prétraitement de séparation du sang. Ladite puce et ledit dispositif peuvent réaliser une séparation rapide d'un très petit échantillon de sang, augmentant l'efficacité de détection de l'échantillon de sang.
PCT/CN2017/100741 2017-07-12 2017-09-06 Puce de prétraitement de séparation du sang et dispositif de séparation du sang WO2019010788A1 (fr)

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CN201710565396.8A CN107262171A (zh) 2017-07-12 2017-07-12 一种血液分离预处理芯片及血液分离装置
CN201710565396.8 2017-07-12

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CN107702973A (zh) * 2017-09-08 2018-02-16 深圳市太赫兹科技创新研究院有限公司 一种全血血浆分离系统及方法
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