WO2018232595A8 - Pcr引物对及其应用 - Google Patents

Pcr引物对及其应用 Download PDF

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Publication number
WO2018232595A8
WO2018232595A8 PCT/CN2017/089196 CN2017089196W WO2018232595A8 WO 2018232595 A8 WO2018232595 A8 WO 2018232595A8 CN 2017089196 W CN2017089196 W CN 2017089196W WO 2018232595 A8 WO2018232595 A8 WO 2018232595A8
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WO
WIPO (PCT)
Prior art keywords
primer
sequence
random sequence
specific sequence
random
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Application number
PCT/CN2017/089196
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English (en)
French (fr)
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WO2018232595A1 (zh
Inventor
杨林
黄国栋
高雅
张艳艳
张海萍
陈芳
蒋浩君
张韶红
王雨倩
谢一帆
蒋慧
Original Assignee
深圳华大智造科技有限公司
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Application filed by 深圳华大智造科技有限公司 filed Critical 深圳华大智造科技有限公司
Priority to EP17914886.1A priority Critical patent/EP3643787A4/en
Priority to US16/624,779 priority patent/US11739319B2/en
Priority to PCT/CN2017/089196 priority patent/WO2018232595A1/zh
Priority to CN201780090470.3A priority patent/CN110603334B/zh
Publication of WO2018232595A1 publication Critical patent/WO2018232595A1/zh
Publication of WO2018232595A8 publication Critical patent/WO2018232595A8/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

提供了PCR引物对及其应用。其中，该PCR引物对包括：第一引物和第二引物，其中，所述第一引物包含第一特异性序列和第一随机序列，所述第一特异性序列位于所述第一引物的3'端，所述第一随机序列位于所述第一引物的5'端，所述第二引物包含第二特异性序列和第二随机序列，所述第二特异性序列位于所述第二引物的3'端，所述第二随机序列位于所述第二引物的5'端，并且，所述第一特异性序列和所述第二特异性序列分别为针对靶序列的上游引物和下游引物，所述第一随机序列和所述第二随机序列反向互补，所述第一特异性序列和所述第一随机序列之间连接有预定酶切位点，所述第二特异性序列和所述第二随机序列之间连接有预定酶切位点。
PCT/CN2017/089196 2017-06-20 2017-06-20 Pcr引物对及其应用 WO2018232595A1 (zh)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP17914886.1A EP3643787A4 (en) 2017-06-20 2017-06-20 PCR PRIMER PAIR AND USE OF IT
US16/624,779 US11739319B2 (en) 2017-06-20 2017-06-20 PCR primer pair and application thereof
PCT/CN2017/089196 WO2018232595A1 (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用
CN201780090470.3A CN110603334B (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/089196 WO2018232595A1 (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

Publications (2)

Publication Number Publication Date
WO2018232595A1 WO2018232595A1 (zh) 2018-12-27
WO2018232595A8 true WO2018232595A8 (zh) 2019-11-28

Family

ID=64736204

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2017/089196 WO2018232595A1 (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

Country Status (4)

Country Link
US (1) US11739319B2 (zh)
EP (1) EP3643787A4 (zh)
CN (1) CN110603334B (zh)
WO (1) WO2018232595A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3643788A4 (en) * 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PAIR OF PRIMERS FOR PCR AND RELATED APPLICATION
CN110029147B (zh) * 2019-01-25 2023-03-24 上海何因生物科技有限公司 一种单管实现连续区域扩增同时降低非特异性扩增的多重pcr方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6830884B1 (en) * 1998-12-15 2004-12-14 Molecular Staging Inc. Method of amplification
US7993839B2 (en) * 2001-01-19 2011-08-09 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
JP5390076B2 (ja) * 2007-05-10 2014-01-15 タカラバイオ株式会社 Ｐｃｒを用いた変異導入方法
WO2009032167A1 (en) * 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
ATE505562T1 (de) * 2007-09-28 2011-04-15 3M Innovative Properties Co Doppel-oligonukleotid- nukleinsäurenachweisverfahren
CN101580829B (zh) * 2009-02-27 2011-05-18 深圳大学 一种基因定点多位点突变的方法
JP6338221B2 (ja) * 2012-10-24 2018-06-06 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸生成物を生成するための、テンプレートスイッチに基づく方法
CN103114131B (zh) * 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
GB2512631A (en) * 2013-04-03 2014-10-08 Rupert Maxwell Gaut Quantitative detection of specific nucleic acid sequences
CN104232628A (zh) * 2013-06-13 2014-12-24 深圳华大基因研究院 一种用于dna靶标序列改造的引物和方法
MX2016007605A (es) * 2013-12-11 2017-01-13 Accuragen Inc Composiciones y metodos para detectar variantes poco frecuentes de una secuencia.
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
CN103820561B (zh) * 2014-03-10 2016-04-20 广西壮族自治区农业科学院园艺研究所 一种柑橘黄龙病亚洲种巢氏pcr扩增检测体系及应用
CN103882530B (zh) * 2014-03-26 2016-02-24 清华大学 用随机序列标记质粒对dna片段进行高通量两端测序的方法
DK3208335T3 (da) * 2014-10-14 2020-08-31 Mgi Tech Co Ltd Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens
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Also Published As

Publication number Publication date
EP3643787A4 (en) 2021-01-06
CN110603334A (zh) 2019-12-20
EP3643787A1 (en) 2020-04-29
US20200216838A1 (en) 2020-07-09
US11739319B2 (en) 2023-08-29
WO2018232595A1 (zh) 2018-12-27
CN110603334B (zh) 2024-01-16

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