WO2018230009A1 - Dispositif d'alimentation en gaz - Google Patents

Dispositif d'alimentation en gaz Download PDF

Info

Publication number
WO2018230009A1
WO2018230009A1 PCT/JP2017/042447 JP2017042447W WO2018230009A1 WO 2018230009 A1 WO2018230009 A1 WO 2018230009A1 JP 2017042447 W JP2017042447 W JP 2017042447W WO 2018230009 A1 WO2018230009 A1 WO 2018230009A1
Authority
WO
WIPO (PCT)
Prior art keywords
upstream
pipe
opening
downstream
small opening
Prior art date
Application number
PCT/JP2017/042447
Other languages
English (en)
Japanese (ja)
Inventor
浩介 石井
有香 吉田
Original Assignee
株式会社Ihi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社Ihi filed Critical 株式会社Ihi
Publication of WO2018230009A1 publication Critical patent/WO2018230009A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F21/00—Dissolving
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/30—Injector mixers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • This disclosure relates to a gas supply device.
  • This application claims priority based on Japanese Patent Application No. 2017-118019 for which it applied to Japan on June 15, 2017, and uses the content here.
  • suspension culture has been performed in order to culture cells in large quantities.
  • the suspension culture is a technique in which any one or more of cells, a mass composed of a plurality of cells, and a cell attached to a carrier such as a microcarrier are suspended and cultured in a culture solution. If the density of the cells in the culture medium increases in suspension culture, the supply of oxygen from the culture medium to the cells may not catch up. For this reason, an air diffuser is installed on the bottom of the culture tank containing the culture solution and cells. And oxygen is supplied to a culture solution through a diffuser tube, and oxygen is dissolved in a culture solution.
  • Patent Document 1 a technology has been developed in which an adjustment tank that contains a culture solution is provided separately from the culture tank, and oxygen is supplied in the adjustment tank through an air diffuser (for example, Patent Document 1).
  • an air diffuser for example, Patent Document 1
  • a large amount of oxygen is supplied from the air diffuser in the adjustment tank, oxygen is dissolved in the culture solution, and the culture solution in which oxygen is dissolved is supplied to the culture vessel.
  • Patent Document 2 since the shape is complicated, it is difficult to reduce the size. For this reason, the ejector of Patent Document 2 cannot be applied to a small-capacity apparatus such as a culture apparatus. Therefore, there is a demand for the development of a technology that can supply fine bubbles with a simple structure.
  • the present disclosure aims to provide a gas supply device capable of supplying fine bubbles with a simple structure.
  • a gas supply device includes a connection pipe in which an opening is formed at one end and the other end, and a through hole is formed in a wall between the one end and the other end.
  • a downstream large opening formed at one end, a small downstream opening smaller than the large downstream opening at the other end, the downstream small opening located in the connecting pipe, and the downstream large opening located outside the connecting pipe; and a downstream pipe
  • An upstream small opening is formed within a predetermined first distance from one end or one end, an upstream large opening larger than the upstream small opening is formed on the other end side from the upstream small opening, and the upstream small opening is connected.
  • a screw groove is formed from an opening formed at the other end of the connection pipe to a predetermined position on the inner peripheral surface of the connection pipe, and at least a part of the outer peripheral surface of the upstream pipe is screwed into the screw groove.
  • a joint may be formed.
  • a thread groove is formed from an opening formed at one end of the connection pipe to a predetermined position on the inner peripheral surface of the connection pipe, and at least a part of the outer peripheral surface of the downstream pipe is screwed into the screw groove. A part may be formed.
  • the upstream pipe may have a small diameter portion whose outer diameter is smaller than that of the downstream small opening from one end to a predetermined second distance larger than the first distance.
  • the upstream small opening may be provided so that at least a part thereof faces the inner peripheral surface of the downstream pipe.
  • the upstream small opening may include a cut formed in the upstream pipe.
  • another gas supply device includes an upstream small opening formed within a predetermined first distance from one end or one end, and an upstream small opening on the other end side from the upstream small opening.
  • An upstream pipe formed with an upstream large opening larger than the opening, a first through hole formed on a side surface, and a liquid having a second through hole formed below the first through hole and above the bottom surface of the side surface
  • a storage section having a main body capable of storing the liquid, a holding section that holds the upstream pipe on the upper surface of the main body so that the upstream small opening is positioned above the lower end of the second through hole in the main body, and contains liquid
  • a pump having a suction side connected to the storage tank and a discharge side connected to the large upstream opening.
  • FIG. 1 is a diagram illustrating a culture system 100.
  • the flow of the culture solution is indicated by solid arrows, and the flow of gas is indicated by broken arrows.
  • the culture system 100 includes a culture tank (reactor) 110, a gas supply device 200, and a culture solution supply pump 120.
  • the culture tank 110 is a sealed container.
  • the culture tank 110 includes a storage portion 112 and a lid portion 114.
  • the container 112 is a substantially conical container whose horizontal cross-sectional area gradually increases from the bottom toward the top.
  • a mixture of a culture solution and cells is accommodated in the accommodating portion 112.
  • the lid part 114 seals the opening formed in the upper part of the accommodating part 112.
  • the culture tank 110 is provided with a separation mechanism (not shown). The culture solution from which the cells have been separated by the separation mechanism is introduced into the gas supply device 200.
  • the gas supply apparatus 200 supplies fine bubbles of culture gas (oxygen, carbon dioxide, etc.) to the culture medium introduced from the culture tank 110 and the new culture liquid supplied by a pump (not shown). A specific configuration of the gas supply device 200 will be described in detail later.
  • the culture solution supply pump 120 supplies the culture solution supplied with the culture gas by the gas supply device 200 (the culture solution in which the culture gas is dissolved) to the culture tank 110 (the bottom of the storage unit 112). Thereby, the culture solution in which the culture gas is dissolved at a high concentration is supplied to the culture tank 110. Therefore, cells can be efficiently cultured in the culture tank 110.
  • the gas supply device 200 that supplies the culture gas to the culture solution will be described.
  • the gas supply device 200 includes an adjustment tank 210 (accommodating tank), a gas supply pump 220, a circulation pump 230 (pump), and a gas supply unit 240.
  • the adjustment tank 210 is a sealed container having a rectangular horizontal cross section.
  • the adjustment tank 210 accommodates the culture solution sent from the culture vessel 110 and a new culture solution supplied by a pump (not shown).
  • the gas supply pump 220 supplies culture gas (oxygen, carbon dioxide, etc.) from a gas supply source (not shown) to the head space of the adjustment tank 210.
  • the gas supply pump 220 supplies the culture gas so that the inside of the adjustment tank 210 is maintained at a predetermined pressure.
  • the circulation pump 230 is connected to the adjustment tank 210 on the suction side.
  • Circulation pump 230 has a discharge side connected to gas supply unit 240 (upstream large opening 254 described later).
  • the circulation pump 230 extracts the culture solution from the adjustment tank 210 and sends the culture solution to the gas supply unit 240 provided in the adjustment tank 210.
  • the circulation pump 230 delivers the culture solution at a flow rate of, for example, 50 ml / min to 200 ml / min.
  • the gas supply unit 240 supplies fine bubbles of the culture gas to the culture solution introduced by the circulation pump 230.
  • the culture solution supplied with the culture gas by the gas supply unit 240 is introduced into the adjustment tank 210. That is, the culture solution circulates through the adjustment tank 210, the circulation pump 230, and the gas supply unit 240.
  • FIG. 2 is a cross-sectional view of the gas supply unit 240 of the first embodiment.
  • the gas supply unit 240 includes an upstream pipe 250, a downstream pipe 260, and a connection pipe 270.
  • Upper portions of the upstream pipe 250, the connection pipe 270, and the downstream pipe 260 are arranged in the head space of the adjustment tank 210.
  • the lower part of the downstream pipe 260 is immersed in the culture solution accommodated in the adjustment tank 210.
  • the upstream pipe 250 is a circular pipe-shaped member whose inner diameter gradually increases from one end to the other end.
  • the upstream pipe 250 is composed of, for example, a polypropylene micropipette tip (for example, a clear tip having a dispensing range of 0.5 ⁇ L to 10 ⁇ L).
  • An upstream small opening 252 and an upstream large opening 254 are formed in the upstream pipe 250.
  • the upstream small opening 252 is formed at one end of the upstream pipe 250.
  • the upstream small opening 252 is an opening having a diameter (size of an XY cross section in FIG. 2) of 0.2 mm or more and 0.5 mm or less.
  • the upstream large opening 254 is formed at the other end of the upstream pipe 250.
  • the upstream large opening 254 is an opening having a diameter (size of an XY cross section in FIG. 2) of 2 mm or more and 10 mm or less.
  • the upstream large opening 254 is larger than the upstream small opening 252.
  • the upstream large opening 254 is connected to the discharge side of the circulation pump 230 described above via a flexible pipe.
  • the downstream pipe 260 is a circular pipe-shaped member whose inner diameter gradually decreases from one end to the other end.
  • the downstream pipe 260 is constituted by, for example, a polypropylene micropipette tip (for example, a clear tip having a dispensing range of 0.5 ⁇ L to 10 ⁇ L).
  • a downstream small opening 262 and a downstream large opening 264 are formed in the downstream pipe 260.
  • the downstream small opening 262 is formed at the other end of the downstream pipe 260.
  • the downstream small opening 262 is an opening having a diameter (size of an XY cross section in FIG. 2) of 0.2 mm or more and 0.5 mm or less.
  • the downstream large opening 264 is formed at one end of the downstream pipe 260.
  • the downstream large opening 264 is an opening having a diameter (size of an XY cross section in FIG. 2) of 2 mm or more and 10 mm or less.
  • the downstream large opening 264 is larger than the downstream small opening 262.
  • the downstream large opening 264 is arranged in the culture solution stored in the adjustment tank 210.
  • the connecting pipe 270 is a circular pipe-shaped member that connects the upstream pipe 250 and the downstream pipe 260.
  • the inner diameter of the connecting pipe 270 is smaller than the upstream large opening 254 of the upstream pipe 250 and the downstream large opening 264 of the downstream pipe 260.
  • the connection pipe 270 is configured by a tube of a member having elasticity (flexibility) (for example, silicon rubber).
  • FIG. 3 is a cross-sectional view of the connecting pipe 270.
  • one end of the upstream pipe 250 is inserted into the opening 272 formed at the other end of the connection pipe 270.
  • a part of the inner peripheral surface of the connection pipe 270 and a part of the outer peripheral surface of the upstream pipe 250 are in close contact with each other.
  • the other end of the downstream pipe 260 is inserted into the opening 274 formed at one end of the connection pipe 270.
  • the inner peripheral surface of the connecting pipe 270 and a part of the outer peripheral surface of the downstream pipe 260 are in close contact with each other.
  • the connecting pipe 270 surrounds the upstream pipe 250 and the downstream pipe 260 by separating the upstream small opening 252 of the upstream pipe 250 and the downstream small opening 262 of the downstream pipe 260 by a distance L in the Z-axis direction in FIG. That is, in the connection pipe 270, the upstream small opening 252 of the upstream pipe 250 and the downstream small opening 262 of the downstream pipe 260 face each other.
  • the distance L is, for example, a predetermined distance that exceeds 0 mm and is 0.5 mm or less.
  • the through hole 276 is a hole having a diameter (XZ cross section in FIG. 3) of 0.01 mm or more and 0.2 mm or less.
  • the upstream pipe 250 is a member whose inner diameter gradually decreases from the other end toward the one end. That is, in the upstream pipe 250, the flow path cross-sectional area (XY cross-sectional area in FIG. 2) gradually decreases from the other end toward the one end. For this reason, the flow rate of the culture solution supplied to the upstream pipe 250 through the upstream large opening 254 increases in the process of passing through the upstream pipe 250. If it does so, the pressure of the culture solution which ejects from the upstream small opening 252 and passes the connection pipe 270 will fall by the venturi effect (ejector effect).
  • connection pipe 270 becomes lower than the outside (head space) of the connection pipe 270, and the gas in the head space is sucked into the connection pipe 270 through the through hole 276. In this manner, culture gas bubbles are supplied to the culture solution in the connection pipe 270.
  • the flow rate of the circulation pump 230 is set to 50 ml / min to 200 ml / min
  • the size of the upstream small opening 252 is set to 0.2 mm to 0.5 mm
  • the size of the through hole 276 is set to 0. It was set to 01 mm or more and 0.2 mm or less.
  • the bubble of the culture gas supplied to a culture solution can be made into a fine bubble (any one or more among a micro bubble, a fine bubble, a nano bubble, and an ultra fine bubble). Therefore, the residence time of the fine bubbles in the culture solution can be increased. That is, the floating time of the fine bubbles on the liquid surface can be extended. This makes it possible to dissolve the culture gas in the culture solution before the fine bubbles reach the liquid level of the culture solution. Therefore, foam generation can be reduced in the culture solution.
  • the gas supply apparatus 200 can supply fine bubbles in a liquid with a simple structure in which the upstream pipe 250 and the downstream pipe 260 are simply connected by the connection pipe 270. Become. Moreover, since the gas supply unit 240 has a simple structure, the gas supply unit 240 can be easily downsized.
  • FIG. 4 is a cross-sectional view of the gas supply unit 340 of the second embodiment.
  • the gas supply unit 340 includes an upstream pipe 350, a downstream pipe 360, and a connection pipe 370. Similar to the gas supply unit 240, the upper part of the upstream pipe 350, the connection pipe 370, and the downstream pipe 360 is disposed in the head space of the adjustment tank 210. The lower part of the downstream pipe 360 is immersed in the culture solution accommodated in the adjustment tank 210.
  • the upstream pipe 350 is a circular pipe-shaped member whose inner diameter gradually increases from one end to the other end.
  • the upstream pipe 350 is composed of, for example, a polypropylene micropipette tip (for example, a clear tip having a dispensing range of 0.5 ⁇ L to 10 ⁇ L).
  • the upstream pipe 350 includes a small diameter part 350a and an enlarged diameter part 350b.
  • the small diameter portion 350a is a portion extending from one end of the upstream pipe 350 over a predetermined second distance M2 on the other end side.
  • the small diameter portion 350a has a shape in which the inner diameter and the outer diameter gradually increase from one end to the other end side.
  • the small diameter portion 350a has a smaller outer diameter than the downstream small opening 362 of the downstream pipe 360 described later.
  • One or more upstream small openings 352 are formed in the small diameter portion 350a.
  • the upstream small opening 352 is formed within a predetermined first distance M1 from one end of the upstream pipe 350 (small diameter portion 350a).
  • the upstream small opening 352 is an opening having a diameter of 0.1 mm to 0.3 mm. The upstream small opening 352 will be described in detail later.
  • the enlarged diameter portion 350b is a portion that is continuous with the small diameter portion 350a and extends from the small diameter portion 350a to the other end.
  • the enlarged diameter portion 350b has a shape in which the inner diameter and the outer diameter gradually increase from one end side toward the other end.
  • An upstream large opening 354 is formed at the other end of the enlarged diameter portion 350b (upstream pipe 350).
  • the upstream large opening 354 is an opening having a diameter (size of an XY cross section in FIG. 4) of 2 mm or more and 10 mm or less.
  • the upstream large opening 354 is larger than the upstream small opening 352.
  • the upstream large opening 354 is connected to the discharge side of the circulation pump 230 described above via a flexible pipe.
  • a protrusion 350c is provided from the other end of the outer peripheral surface of the enlarged diameter portion 350b to a predetermined distance Mc.
  • the outer diameter of the protrusion 350c is constant over the Z-axis direction in FIG.
  • a threaded portion 350ca that is threadedly engaged with a thread groove 372a described later is formed on the outer peripheral surface of the protruding portion 350c.
  • the downstream pipe 360 is a circular pipe-shaped member whose inner diameter gradually decreases from one end to the other end.
  • a downstream small opening 362 and a downstream large opening 364 are formed in the downstream pipe 360.
  • the downstream small opening 362 is formed at the other end of the downstream pipe 360.
  • the downstream small opening 362 is an opening having a diameter (size of XY cross section in FIG. 4) of 0.7 mm or more and 1.3 mm or less.
  • the downstream large opening 364 is formed at one end of the downstream pipe 360.
  • the downstream large opening 364 is an opening having a diameter (size of an XY cross section in FIG. 4) of 2 mm or more and 10 mm or less.
  • the downstream large opening 364 is arranged in the culture solution stored in the adjustment tank 210.
  • the downstream large opening 364 is larger than the downstream small opening 362.
  • the downstream pipe 360 is composed of, for example, a polypropylene micropipette tip (for example, a blue tip having a dispensing range of 100 ⁇ L to 1000 ⁇ L).
  • connection pipe 370 is a circular pipe-shaped member that connects the upstream pipe 350 and the downstream pipe 360.
  • the inner diameter of the connection pipe 370 is smaller than the downstream large opening 364 of the downstream pipe 360.
  • the inner diameter and outer diameter of the connecting pipe 370 are constant over the Z-axis direction in FIG.
  • the connection pipe 370 is made of, for example, a polypropylene pipe.
  • a screw groove 372a is formed from the opening 372 to a predetermined position on the inner peripheral surface of the connection pipe 370.
  • the screw groove 372a is screwed with the screwing portion 350ca of the upstream pipe 350.
  • the length of the screw groove 372a in the Z-axis direction in FIG. 4 is longer than the distance Mc of the screwing portion 350ca.
  • the other end of the downstream pipe 360 is inserted into the opening 374 formed at one end of the connection pipe 370.
  • a part of the inner peripheral surface of the connection pipe 370 and a part of the outer peripheral surface of the downstream pipe 360 are brought into close contact with each other.
  • One or a plurality of through-holes 376 are formed in the wall constituting the connection pipe 370 between the place where the upstream pipe 350 is closely attached and the place where the downstream pipe 360 is closely attached.
  • the through hole 376 is a hole having a diameter (XZ cross section in FIG. 3) of 0.2 mm or more and 3 mm or less.
  • FIG. 5 is a diagram illustrating the upstream small opening 352 of the upstream pipe 350, the downstream small opening 362 of the downstream pipe 360, and the connection pipe 370.
  • the flow of the culture solution is indicated by a solid line arrow, and the flow of the culture gas is indicated by a broken line arrow.
  • the connecting pipe 370 has an upstream pipe 350 such that the upstream small opening 352 is located in the downstream pipe 360, and the inner peripheral surface of the downstream pipe 360 and the outer peripheral surface of the small diameter portion 350a are separated from each other. And the downstream pipe 360 is surrounded. Then, the upstream small opening 352 is arranged facing the inner peripheral surface of the downstream pipe 360. Therefore, the culture solution ejected from the upstream small opening 352 collides with the inner peripheral surface of the downstream pipe 360. When the culture solution collides with the downstream pipe 360, the culture gas is entrained. As a result, the culture gas can be efficiently taken into the culture solution.
  • the gas supply unit 340 of the second embodiment has a simple structure in which the upstream pipe 350 and the downstream pipe 360 are simply connected by the connection pipe 370 and can supply fine bubbles into the liquid. It becomes possible.
  • the upstream pipe 350 has a threaded portion 350ca, and the connection pipe 370 has a screw groove 372a, so that the distance M3 between the outer peripheral surface of the upstream pipe 350 (small diameter portion 350a) and the edge of the downstream small opening 362. Can be changed between 0.01 mm and 0.3 mm.
  • This makes it possible to adjust the flow path cross-sectional area of the culture gas (the difference between the size of the downstream small opening 362 and the XY cross-sectional area in FIG. 5 of the small diameter portion 350a). Therefore, the supply amount of the culture gas to the culture solution can be adjusted.
  • the flow rate of the circulation pump 230 is set to 50 ml / min to 200 ml / min
  • the size of the upstream small opening 352 is set to 0.1 mm to 0.3 mm
  • the distance M3 is set to 0.01 mm to 0.00 mm. 3 mm or less.
  • one or both of the screwing portion 350ca and the screw groove 372a may be provided with a restriction portion that restricts the movement of the upstream pipe 350 relative to the connection pipe 370 immediately before the distance M3 becomes zero. Good.
  • FIG. 6A is a diagram illustrating the upstream pipe 450 of the first modification.
  • FIG. 6B is a diagram illustrating the upstream pipe 550 of the second modification.
  • symbol is attached
  • a cut 452 (upstream small opening) is formed in the small diameter portion 350a from one end to the other end side.
  • the length of the cut 452 (the length in the Z-axis direction in FIG. 6A) is not less than 0.5 mm and not more than 2 mm.
  • the upstream pipe 450 is arranged so that the cut 452 faces the inner peripheral surface of the downstream pipe 360.
  • the upstream pipe 450 is made of a material having elasticity (flexibility). Therefore, when the culture solution passes, the cut 452 is opened, and the culture solution is sprayed through the cut 452.
  • the notch 452 is opened to the extent that the contaminant passes due to the movement of the contaminant accompanying the flow of the culture solution (the degree of opening of the notch 452 is growing). Then, impurities are discharged to the downstream pipe 360 through the cut 452. Therefore, it is possible to avoid a situation where the upstream pipe 450 is blocked by foreign substances.
  • the upstream pipe 550 of the second modified example has an opening 552 (upstream small opening) and a notch 554 (upstream small opening) in the small diameter portion 350a.
  • the notch 554 is formed from one end of the small diameter portion 350a toward the other end side.
  • the opening 552 is continuous with the other end of the notch 554.
  • the opening 552 is formed continuously at the two ends of the notch 554. That is, two openings 552 are formed in the small diameter portion 350a.
  • the length of the notch 554 (the length in the Z-axis direction in FIG. 6B) is not less than 0.5 mm and not more than 2 mm.
  • the upstream pipe 550 is arranged so that the opening 552 and the notch 554 face the inner peripheral surface of the downstream pipe 360.
  • the culture solution passes, the culture solution is sprayed through the opening 552. Then, the culture solution collides with the inner peripheral surface of the downstream pipe 360. Then, when the culture solution collides, the culture gas is involved, and the culture gas can be efficiently taken into the culture solution.
  • the upstream pipe 550 is made of a material having elasticity (flexibility). Therefore, similarly to the upstream pipe 450 of the first modified example, when impurities (solid matter) are mixed in the culture solution, the extent of the impurities passing by the movement of the impurities accompanying the flow of the culture solution.
  • the notch 554 is opened (the opening degree of the notch 554 increases). Then, impurities are discharged to the downstream pipe 360 through the cut 554. Therefore, it is possible to avoid a situation where the upstream pipe 550 is blocked by foreign substances.
  • FIG. 7 is a cross-sectional view of the gas supply unit 640 of the third embodiment.
  • the flow of the culture solution is indicated by a solid line arrow, and the flow of the culture gas is indicated by a broken line arrow.
  • the gas supply unit 640 includes an upstream pipe 650 and a storage section 660.
  • the gas supply unit 640 is disposed in the head space of the adjustment tank 210.
  • symbol is attached
  • the upstream pipe 650 is a circular pipe-shaped member whose inner diameter gradually increases from one end to the other end.
  • the upstream pipe 650 is composed of, for example, a polypropylene micropipette tip (for example, a clear tip having a dispensing range of 0.5 ⁇ L to 10 ⁇ L).
  • An upstream small opening 252 and an upstream large opening 254 are formed in the upstream pipe 650.
  • the upstream small opening 252 is formed at one end of the upstream pipe 650.
  • the upstream small opening 252 is an opening having a diameter (size of an XY cross section in FIG. 7) of 0.2 mm or more and 0.5 mm or less.
  • the upstream large opening 254 is formed at the other end of the upstream pipe 650.
  • the upstream large opening 254 is an opening having a diameter (size of an XY cross section in FIG. 7) of 2 mm or more and 10 mm or less.
  • the upstream large opening 254 is larger than the upstream small opening 252.
  • the upstream large opening 254 is connected to the discharge side of the circulation pump 230 described above via a flexible pipe.
  • a protrusion 650a is provided from the other end of the outer peripheral surface of the upstream pipe 650 to a predetermined distance Nc.
  • the outer diameter of the protrusion 650a is constant over the Z-axis direction in FIG.
  • a threaded portion 650aa that is threadedly engaged with a thread groove 668a described later is formed on the outer peripheral surface of the protruding portion 650a.
  • the storage unit 660 includes a main body 662.
  • the main body 662 is a hollow container. More specifically, the main body 662 is a cylinder having a top surface 662a having a planar shape and a bottom portion 662b having a hemispherical shape.
  • a first through hole 664 is formed in the side surface 662c of the main body 662. The size of the XZ cross section in FIG. 7 of the first through hole 664 is 0.2 mm or more and 3 mm or less.
  • a second through hole 666 is formed on the side surface 662c of the main body 662 below the first through hole 664 and above the bottom 662b (bottom surface). The size of the XZ cross section in FIG. 7 of the second through hole 666 is 1.5 mm or more and 5 mm or less.
  • a third through hole 668 is formed in the upper surface 662a of the main body 662.
  • the third through hole 668 is formed with a thread groove 668a (holding portion).
  • the thread groove 668a is screwed with the screwing portion 650aa of the upstream pipe 650.
  • the upstream pipe 650 is fixed to the upper surface 662a of the main body 662 by the thread groove 668a so that the upstream small opening 252 is located above the lower end of the second through hole 666 in the main body 662.
  • the upstream pipe 650 is a member whose inner diameter gradually decreases from the other end toward the one end. For this reason, the flow rate of the culture solution supplied to the upstream pipe 650 through the upstream large opening 254 increases in the process of passing through the upstream pipe 650. Then, the pressure of the culture solution ejected from the upstream small opening 252 decreases due to the venturi effect (ejector effect).
  • the inside of the storage section 660 (main body 662) becomes lower in pressure than the head space, and the gas in the head space is sucked into the main body 662 through the first through hole 664.
  • the culture liquid ejected from the upstream small opening 252 collides with the culture liquid stored in the main body 662, the culture gas in the main body 662 is entrained, and bubbles of the culture gas are supplied to the culture liquid.
  • the culture solution in which the culture gas is dissolved is sent to the outside (regulation tank 210) through the second through hole 666.
  • the thread groove 668a fixes the upstream pipe 650 so that the distance between the lower end of the second through hole 666 and the upstream small opening 252 is a predetermined distance N that is greater than 0 mm and equal to or less than 0.5 mm. That is, the thread groove 668a fixes the upstream pipe 650 so that the upstream small opening 252 and the liquid level in the main body 662 are separated by a distance N.
  • the distance N is greater than 0 mm and not greater than 0.5 mm and the flow rate of the circulation pump 230 to be not less than 50 ml / min and not more than 200 ml / min, the bubbles of the culture gas supplied to the culture medium are fine bubbles. can do.
  • FIG. 8 is a cross-sectional view of a gas supply unit 740 according to a third modification.
  • symbol is attached
  • the gas supply unit 740 includes an upstream pipe 250, a guide part 750, and a storage part 760.
  • the guide portion 750 is a circular tube-shaped member that surrounds the upstream pipe 250 from one end of the upstream pipe 250 to a predetermined range.
  • the guide part 750 fixes the upstream pipe 250 so that one end is located below the upstream small opening 252 of the upstream pipe 250.
  • the guide part 750 includes an enlarged diameter part 752 and an identical diameter part 754.
  • the enlarged diameter portion 752 has a shape in which the inner diameter and the outer diameter gradually increase from one end to the other end side.
  • An opening 752 a is formed at one end of the enlarged diameter portion 752.
  • the opening 752a is provided so as to be positioned below the lower end of the second through hole 666 of the storage portion 760 described later.
  • the same diameter portion 754 is continuous with the enlarged diameter portion 752 and has a shape having a constant diameter from the enlarged diameter portion 752 to the other end.
  • a plate member 754 a is provided in the opening at the other end of the same diameter portion 754 across the edge of the opening.
  • a first through hole 754aa is formed in the plate member 754a.
  • the upstream pipe 250 is inserted into the first through hole 754aa.
  • the upstream pipe 250 is fixed to the guide portion 750 in a state where the upstream pipe 250 is inserted into the first through hole 754aa.
  • the plate member 754a has a second through hole 754ab. Further, on the outer peripheral surface of the same diameter portion 754, a screwing portion 754b screwed with the screw groove 668a is formed.
  • the storage unit 760 includes a main body 762.
  • the main body 762 differs from the main body 662 only in that the first through hole 664 is not formed.
  • the screw groove 668a formed in the third through hole 668 is screwed with the screw portion 754b of the guide portion 750.
  • the guide portion 750 has an opening 752a positioned below the lower end of the second through hole 666 in the main body 762 and a small upstream opening 252 positioned above the lower end of the second through hole 666 by the thread groove 668a.
  • the main body 762 is fixed to the upper surface 662a.
  • the screw groove 668a fixes the guide portion 750 so that the distance between the lower end of the second through hole 666 and the upstream small opening 252 is a predetermined distance N that is greater than 0 mm and equal to or less than 0.5 mm.
  • the gas supply unit 740 of the third modified example can make the bubbles of the culture gas supplied to the culture solution into fine bubbles.
  • the configuration in which the gas supply unit 740 includes the guide portion 750 makes it possible to stabilize the distance from the upstream small opening 252 to the liquid level. Thereby, a microbubble can be stably supplied to a culture solution.
  • the configuration in which the through hole 276 is always open has been described as an example.
  • the through hole 276 is closed when the culture solution is not supplied, and may be opened by the negative pressure in the connection pipe 270 when the culture solution is supplied.
  • the configuration in which the upstream small opening 352 is formed on the side surface of the small diameter portion 350a of the upstream pipe 350 has been described as an example.
  • the upstream small opening 352 may be formed at the end (one end) of the small diameter portion 350a.
  • a thread groove may be formed from an opening 374 formed at one end of the connection pipe 370 to a predetermined position.
  • the distance M3 between the outer peripheral surface of the upstream pipe 350 (small diameter portion 350a) and the edge of the downstream small opening 362 can be changed.
  • the configuration in which the notches 452 and 554 are always open has been described as an example.
  • the notches 452, 554 are closed when the culture solution is not supplied, and may be opened at the supply pressure when the culture solution is supplied.
  • the configuration in which one notch 452 is formed in the upstream pipe 450 has been described as an example.
  • two or more notches 452 may be formed in the upstream pipe 450. In this case, the notches 452 may cross each other.
  • two or more cuts 554 may be formed in the upstream pipe 550. In this case, the notches 554 may cross each other.
  • the configuration in which the opening 552 is formed at the end of the notch 554 has been described as an example.
  • the opening 552 may be formed in the middle of the notch 554.
  • the number of openings 552 is not limited.
  • a cut 452 may be formed in the upstream pipe 650. Further, an opening 552 and a cut 554 may be formed in the upstream pipe 650. Similarly, in the third modified example, a cut 452 may be formed in the upstream pipe 250. Further, an opening 552 and a cut 554 may be formed in the upstream pipe 250.
  • the upstream pipe 250, the downstream pipe 260, the connection pipe 270, the upstream pipe 350, the downstream pipe 360, the connection pipe 370, the upstream pipe 450, the upstream pipe 550, the upstream pipe 650, the storage section 660, the guide section 750, and the storage section The case where 760 has a circular tube shape has been described as an example. However, these may be tube shapes. Further, the case where the upstream pipes 250, 350, 450, 550, and 650 have a circular pipe shape whose inner diameter gradually increases from one end to the other end has been described as an example.
  • the shapes of the upstream pipes 250, 350, 450, 550, 650 are not limited as long as the upstream large openings 254, 354 are larger than the upstream small openings 252, 352, the notches 452, the openings 552, and the notches 554.
  • the case where the downstream pipes 260 and 360 have a circular pipe shape whose inner diameter gradually decreases from one end to the other end has been described as an example.
  • the shape of the downstream pipes 260 and 360 is not limited as long as the downstream large openings 264 and 364 are larger than the downstream small openings 262 and 362.
  • the gas supply device 200 has been described by taking as an example the configuration in which fine bubbles are supplied to the culture solution.
  • the gas supply device 200 may supply fine bubbles to a liquid other than the culture solution.
  • the present disclosure can be used for a gas supply device.
  • 200 gas supply device 210 adjustment tank (container tank), 230 circulation pump (pump), 250 upstream pipe, 252 upstream small opening, 254 upstream large opening, 260 downstream pipe, 262 downstream small opening, 264 downstream large opening, 270 connection Pipe, 272 opening, 274 opening, 276 through hole, 350 upstream pipe, 350a small diameter part, 350ca screwing part, 352 upstream small opening, 354 upstream large opening, 360 downstream pipe, 362 downstream small opening, 364 downstream large opening, 370 Connecting pipe, 372 opening, 372a screw groove, 376 through hole, 450 upstream pipe, 452 notch (upstream small opening), 550 upstream pipe, 552 opening (upstream small opening), 554 notching (upstream small opening), 650 upstream pipe, 660 reservoir, 662 body, 662a top surface, 662b bottom ( Surface) 662c side, 664 the first through hole, 666 a second through hole, 668a thread groove (holding portion), 760 reservoir, 762 body

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif d'alimentation en gaz qui comprend: un tuyau de raccordement (270) dans lequel une ouverture est formée sur les deux extrémités et des trous traversants sont formés dans une paroi entre les deux extrémités; un tuyau d'écoulement descendant (260) dans lequel une grande ouverture d'écoulement descendant (264) est formée dans une extrémité et une petite ouverture d'écoulement descendant (262) qui est plus petite que la grande ouverture d'écoulement descendant est formée dans l'autre extrémité, la petite ouverture d'écoulement descendant étant positionnée à l'intérieur du tuyau de raccordement, et la grande ouverture d'écoulement descendant étant positionnée à l'extérieur du tuyau de raccordement; un tuyau d'écoulement ascendant (250) qui est disposé séparément du tuyau d'écoulement descendant et dans lequel une ouverture de petit débit (252) est formée dans une extrémité ou à l'intérieur d'une première distance prescrite à partir d'une extrémité et une ouverture d'écoulement de haut débit (254) qui est plus grande que la petite ouverture d'écoulement vers le haut est formée plus vers l'autre côté d'extrémité que l'ouverture de petit débit, avec la petite ouverture d'écoulement vers le bas faisant face à l'ouverture d'écoulement bas dans le tuyau de raccordement ou étant positionné à l'intérieur du tuyau d'écoulement descendant à l'intérieur du tuyau de raccordement, et l'ouverture d'écoulement de haut débit étant positionnée à l'extérieur du tuyau de raccordement; un réservoir de réception destiné à recevoir un liquide; et une pompe dont le côté aspiration est relié au réservoir de réception et dont le côté de décharge est relié à l'ouverture de haut débit.
PCT/JP2017/042447 2017-06-15 2017-11-27 Dispositif d'alimentation en gaz WO2018230009A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017-118019 2017-06-15
JP2017118019A JP6988183B2 (ja) 2017-06-15 2017-06-15 ガス供給装置

Publications (1)

Publication Number Publication Date
WO2018230009A1 true WO2018230009A1 (fr) 2018-12-20

Family

ID=64659085

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2017/042447 WO2018230009A1 (fr) 2017-06-15 2017-11-27 Dispositif d'alimentation en gaz

Country Status (2)

Country Link
JP (1) JP6988183B2 (fr)
WO (1) WO2018230009A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54106050U (fr) * 1978-01-12 1979-07-26
US5520856A (en) * 1994-03-15 1996-05-28 The Boc Group Plc Gas dissolving
EP1002567A1 (fr) * 1998-11-19 2000-05-24 The BOC Group plc Dissolution de gaz
JP2010201295A (ja) * 2009-02-27 2010-09-16 Tetsuhiko Fujisato 過飽和洗浄水生成器
US20150266758A1 (en) * 2014-03-18 2015-09-24 William R. Nelson Water treatment system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54106050U (fr) * 1978-01-12 1979-07-26
US5520856A (en) * 1994-03-15 1996-05-28 The Boc Group Plc Gas dissolving
EP1002567A1 (fr) * 1998-11-19 2000-05-24 The BOC Group plc Dissolution de gaz
JP2010201295A (ja) * 2009-02-27 2010-09-16 Tetsuhiko Fujisato 過飽和洗浄水生成器
US20150266758A1 (en) * 2014-03-18 2015-09-24 William R. Nelson Water treatment system

Also Published As

Publication number Publication date
JP6988183B2 (ja) 2022-01-05
JP2019000808A (ja) 2019-01-10

Similar Documents

Publication Publication Date Title
KR0173996B1 (ko) 기액용해 혼합방법 및 장치
US20080197516A1 (en) Micro-Bubble Generator, Vortex Breakdown Nozzle for Micro-Bubble Generator, Vane Swirler for Micro-Bubble Generator, Micro-Bubble Generating Method, and Micro-Bubble Applying Device
JP2017144428A (ja) 超音波霧化装置
EP0546033A1 (fr) Aeration de liquides
JP2009240988A (ja) 微細気泡供給装置の気液分離器
US20160236160A1 (en) Microbubble generating device and contaminated water purifying system provided with microbubble generating device
WO1995034370A1 (fr) Tube de dispersion gazeuse pour barbotage de gaz dans liquide, procede de barbotage gaz dans liquide et dispositif utilisant ce procede
JP2012250138A (ja) 微細気泡生成ノズルおよび微細気泡生成装置
WO2018230009A1 (fr) Dispositif d'alimentation en gaz
WO2015072461A1 (fr) Dispositif générant un liquide microbicide
KR101567459B1 (ko) 기액반응기
JP2009208051A (ja) 水素水及び水素水生成装置
JP6377569B2 (ja) 流体吹込装置及びこれを用いた化学反応装置
JP2003245533A (ja) 超微細気泡発生装置
CN111615424A (zh) 微小气泡生成方法和微小气泡生成装置
US11202998B2 (en) Systems and methods for gas disposal
JP2019013882A (ja) ガス分離装置
US20200156018A1 (en) Fine bubble generating method and fine bubble generating apparatus
US20240198300A1 (en) Device and method for dispersing gases into liquids
JP2009112947A (ja) 処理液の製造装置及び製造方法
US6892968B1 (en) Nozzle assembly for use in the treatment of waste water
US4961882A (en) Fine bubble generator and method
JPH05123554A (ja) 気液接触装置およびオゾン水製造装置
JP4616142B2 (ja) 気液接触用ガス分散管と、これを用いる気液接触方法及び装置並びに排ガスの処理方法及び装置
WO2020028646A1 (fr) Appareil et procédé d'expansion de nano-bulles dans un support liquide

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17913805

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17913805

Country of ref document: EP

Kind code of ref document: A1