WO2018228018A1 - 一种带电防粘连组织修复膜及其制备方法 - Google Patents

一种带电防粘连组织修复膜及其制备方法 Download PDF

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WO2018228018A1
WO2018228018A1 PCT/CN2018/080426 CN2018080426W WO2018228018A1 WO 2018228018 A1 WO2018228018 A1 WO 2018228018A1 CN 2018080426 W CN2018080426 W CN 2018080426W WO 2018228018 A1 WO2018228018 A1 WO 2018228018A1
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charged anti
film
adhesion tissue
adhesion
tissue repairing
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PCT/CN2018/080426
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English (en)
French (fr)
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张学慧
邓旭亮
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北京大学口腔医学院
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/222—Gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants

Definitions

  • the invention relates to the technical field of surgical repair materials, in particular to a charged anti-adhesion tissue repair film and a preparation method thereof.
  • Implantation repair is currently the primary means of treating large-scale tissue defects. Artificial repair materials have received extensive attention in tissue repair materials due to their wide range of sources, controllable performance and low cost. However, the existing tissue repair materials have poor biological activity, especially for the non-absorbable implant materials, which are difficult to remove the material and tissue adhesion, thereby causing side effects, and finally lead to poor clinical long-term repair effect.
  • the tissue repair process includes skin wound healing, nerve repair, bone defect repair, etc.
  • damage potentials There are damage potentials.
  • a large number of studies have confirmed that the damage potential plays an important role in tissue repair. Therefore, for tissue repair materials, charging the material will help promote tissue fast. Healing and functional repair.
  • electroactive materials have become a research hotspot and trend, and also a new idea for the design of tissue repair materials.
  • Ferroelectric polymers such as polyvinylidene fluoride (PVDF) and polyvinylidene fluoride-trifluoroethylene (P(VDF-TrFE)) are widely used because of their inherent spontaneous polarization properties and biocompatibility.
  • PVDF polyvinylidene fluoride
  • PVDF-TrFE polyvinylidene fluoride-trifluoroethylene
  • the existing tissue repair materials have poor biological activity, especially for the non-absorbable implant materials in the same kind, the defects of materials and tissue adhesion are not easy to be removed, thereby causing side effects, and finally the clinical long-term repair effect is poor.
  • the present invention is to solve the technical problems of non-tissue-inducing activity, tissue adhesion, and the like existing in the prior art, and to provide a repairing film material which is charged and prevents tissue adhesion.
  • the present invention provides a charged anti-adhesion tissue repair film comprising a ferroelectric polymer polyvinylidene fluoride, a ferroelectric polymer polyvinylidene fluoride-trifluoroethylene (P(VDF-TrFE)) or a combination thereof. Things.
  • the charged anti-adhesion tissue repair film is a film material having a thickness of 10 ⁇ m to 300 ⁇ m.
  • the charged anti-adhesion tissue repair film further comprises one or more of chitosan, hyaluronic acid, collagen, gelatin, and silk fibroin.
  • the charged anti-adhesion tissue repair film further contains one or more of L-polylactic acid, polylactic acid-glycolic acid copolymer and polycaprolactone.
  • the invention also provides a preparation method of a charged anti-adhesion tissue repairing membrane, which is completed by an organic solvent dissolution and a casting method.
  • the method for preparing the charged anti-adhesion tissue repairing film comprises the following steps: (1) taking a polymer, adding an organic solvent, stirring for 3 h to 8 h to completely dissolve, to obtain a polymer solution; (2) taking the step (1) The obtained solution is cast in a casting machine, and the obtained cast film is dried at a temperature of 40 ° C to 100 ° C to completely evaporate the solvent to obtain a tissue repair film material having a film thickness of 1 ⁇ m to 200 ⁇ m; (3) The film material obtained in the step (2) is subjected to polarization treatment, and the polarization parameters include: a polarization voltage of 1 kV to 30 kV, a polarization distance of 0 mm to 50 mm, a polarization temperature of 25 ° C to 150 ° C, and a polarization time of From 1 min to 60 min, a charged anti-adhesion tissue repair film was obtained.
  • the invention adopts a ferroelectric polymer as a main component, and the formed film material has uniform structure, stable performance and strong clinical operability. Moreover, by incorporating a biocompatible polymer component, the biological activity of the material can be improved while maintaining good flexibility of the material.
  • the film material prepared by the invention has a certain polarization charge on the surface of the film material due to the inherent spontaneous polarization property of the material, and can maintain good stability.
  • the charged membrane material prepared by the invention has good affinity with osteoblast-related cells, can promote adhesion, proliferation and differentiation of osteoblasts; and the material can be well induced to induce tissue repair after implantation in vivo. .
  • the charged film material prepared by the invention has strong hydrophobicity (water contact angle is about 100 degrees), does not adhere to the tissue after being implanted in the body for tissue repair, is easy to take out, prevents adhesion, and greatly Side effects caused by material residue are avoided.
  • the preparation process adopted by the invention is simple, operability is strong, and can be used for industrial production.
  • the charged anti-adhesion tissue repair membrane provided by the invention has good performance in both macroscopic properties and microstructure, and is suitable for adhesion, growth and differentiation of osteogenic cells during bone repair.
  • the electrical microenvironment does not cause tissue adhesion and avoids material residue; in addition, the preparation method can make the tissue repair membrane have good bioreactivity and help to further improve the tissue repair effect.
  • FIG. 1 is a scanning electron micrograph of a tissue repair membrane material according to Embodiment 1 of the present invention.
  • FIG. 2 is a hysteresis loop diagram of the tissue repair membrane material according to Embodiment 1 of the present invention (remaining polarization intensity is about 5.5 ⁇ C/cm 2 );
  • FIG. 3 is a stress-strain curve of a tissue repair membrane material according to Embodiment 1 of the present invention.
  • Example 5 is a scanning electron micrograph of an in vitro bone marrow mesenchymal stem cell culture tissue of the tissue repair membrane material according to Example 1 of the present invention
  • FIG. 6 is a photomicrograph of a 12-week tissue section of a bone repairing defect of the tissue repairing membrane material according to the embodiment 1 of the present invention (the repairing membrane does not adhere to the new bone tissue, and is easy to take out).
  • the invention provides a charged anti-adhesion tissue repairing film and a preparation method thereof.
  • the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
  • the film material obtained in the step (2) is subjected to polarization treatment, and the polarization parameters include: a polarization voltage of 15 kV, a polarization distance of 15 mm, a polarization temperature of 25 ° C, and a polarization time of 30 min.
  • the main component of the charged anti-adhesion tissue repair membrane material obtained by the above steps was a polymer P (VDF-TrFE) which was a film-like material and had a film thickness of 60 ⁇ m.
  • the film material obtained in the step (2) is subjected to polarization treatment, and the polarization parameters include: a polarization voltage of 13 kV, a polarization distance of 15 mm, a polarization temperature of 80 ° C, and a polarization time of 20 min.
  • the main component of the charged anti-adhesion tissue repair membrane material obtained by the above steps is polymer PVDF, which is a film-like material having a film thickness of 50 ⁇ m.
  • the film material obtained in the step (2) is subjected to polarization treatment, and the polarization parameters include: a polarization voltage of 18 kV, a polarization distance of 20 mm, a polarization temperature of 60 ° C, and a polarization time of 30 min.
  • the piezoelectric active bone repair composite obtained by the above steps contains a polymer P (VDF-TrFE) and a polymer PLLA, which is a film-like material with a film thickness of 70 ⁇ m, a polymer P (VDF-TrFE) and a polymer PLLA.
  • the mass content of the composite membrane material was 50% each.
  • the film material obtained in the step (2) is subjected to polarization treatment, and the polarization parameters include: a polarization voltage of 15 kV, a polarization distance of 12 mm, a polarization temperature of 40 ° C, and a polarization time of 40 min.
  • the piezoelectric active bone repair composite obtained by the above steps contains a polymer P (VDF-TrFE) and a natural polymer chitosan, which is a film-like material having a film thickness of 80 ⁇ m, a polymer P (VDF-TrFE) and a natural one.
  • the mass content of the polymer chitosan in the composite membrane material was 50% each.

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Abstract

一种带电防粘连组织修复膜及其制备方法，带电防粘连组织修复膜含有铁电高分子聚偏氟乙烯、铁电高分子聚偏氟-三氟乙烯或两者的复合物。形成的膜材料结构均一，性能稳定，进行组织修复后不会与组织发生粘连，容易取出。

Description

一种带电防粘连组织修复膜及其制备方法 技术领域
本发明涉及外科手术用修复材料技术领域，特别涉及一种带电防粘连组织修复膜及其制备方法。
背景技术
植入修复是目前治疗大范围组织缺损的主要手段。人工修复材料由于来源广泛、性能可控及成本低，在组织修复材料中受到广泛关注。然而，现有组织修复材料存在生物活性差，尤其对于不可吸收性植入材料存在材料与组织粘连不易去除、由此引起副作用等缺陷，最终导致临床长期修复效果差。
组织修复过程包括皮肤创伤愈合、神经修复、骨缺损修复等均存在损伤电位，大量研究证实损伤电位对组织修复具有重要作用，因此，对于组织修复材料而言，使材料带电将有利于促进组织快速愈合和功能修复。近年来，电活性材料成为研究热点和趋势，也是组织修复材料设计的新思路。
铁电聚合物如聚偏氟乙烯(Polyvinylidene fluoride，PVDF)、聚偏氟-三氟乙烯(P(VDF-TrFE))等因具有内在的自发极化性能和生物相容性，目前广泛用于生物医用研究领域，同时具有良好的柔韧性和可加工性，使其具备良好的临床可操作性，成为当前带电生物医用材料研究的主流方向。
现有组织修复材料存在生物活性差，尤其对于同类不可吸收性植入材料存在材料与组织粘连不易去除、由此引起副作用等缺陷，最终导致临床长期修复效果差。
发明内容
本发明就是为了解决现有方法中存在的无组织诱导活性、易发生组织黏连等技术问题，提供一种带电且防止组织粘连的修复膜材料。
为此，本发明提供一种带电防粘连组织修复膜，其含有铁电高分子聚偏氟乙烯、铁电高分子聚偏氟-三氟乙烯(P(VDF-TrFE))或两者的复合物。
优选的，带电防粘连组织修复膜为薄膜材料，其厚度为10μm～300μm。
优选的，带电防粘连组织修复膜还含有壳聚糖、透明质酸、胶原、明胶、丝素蛋白中的一种或多种。
优选的，带电防粘连组织修复膜还含有左旋聚乳酸、聚乳酸-羟基乙酸共聚物和聚己内酯中的一种或多种。
本发明同时提供一种带电防粘连组织修复膜的制备方法，其通过有机溶剂溶解以及流延法完成。
优选的，带电防粘连组织修复膜的制备方法包括如下步骤：(1)取聚合物，加入有机溶剂，搅拌3h～8h使其完全溶解，得到聚合物溶液；(2)取所述步骤(1)所得溶液于流延机中流延，将所得流延膜置于40℃～100℃温度下烘干，使溶剂完全挥发，得到一种组织修复膜材料，膜厚为1μm～200μm；(3)将所述步骤(2)所得膜材料经过极化处理，极化参数包括：极化电压为1kV～30kV，极化距离为0mm～50mm，极化温度为25℃～150℃，极化时间为1min～60min，得到一种带电防粘连组织修复膜。
本发明效果在于，
(1)本发明采用铁电聚合物为主要成分，形成的膜材料结构均一、性能稳定，临床可操作性强。而且可以通过掺入生物相容性聚合物成分，在保持材料良好柔韧性的基础上，提高材料的生物活性。
(2)本发明所制得的膜材料由于材料内在的自发极化特性，经过极化处理后可使膜材料表面带有一定的极化电荷，并可保持良好稳定性。
(3)本发明所制得的带电膜材料与成骨相关细胞具有良好的亲和性，可促进成骨细胞的粘附、增殖和分化；体内植入后可见材料具有良好的诱导组织修复性能。
(4)本发明所制得的带电膜材料具有较强的疏水性(水接触角约为 100度)，植入体内进行组织修复后不会与组织发生粘连，容易取出，防止粘连，极大地避免了材料残留引发的副作用。
(5)本发明所采用的制备工艺简单，可操作性强，能用于工业化生产。
综上所述，本发明所提供的带电防粘连组织修复膜在宏观性能和微观结构上均具有良好的性能，在骨修复过程中，为成骨相关细胞的粘附、生长和分化提供更适宜的电学微环境，同时不会发生组织粘连，避免材料残留；此外，该制备方法可使得组织修复膜具有良好的生物反应性，有助于进一步改善组织修复效果。
附图说明
图1为本发明实施例1所述组织修复膜材料的扫描电子显微镜照片；
图2为本发明实施例1所述组织修复膜材料的电滞回线图(剩余极化强度大约为5.5μC/cm 2)；
图3为本发明实施例1所述组织修复膜材料的应力-应变曲线；
图4为本发明实施例1所述组织修复膜材料的水接触角图片；
图5为本发明实施例1所述组织修复膜材料的体外骨髓间充质干细胞培养1天后的扫描电子显微镜照片；
图6为本发明实施例1所述组织修复膜材料的体内修复骨缺损12周组织切片的光镜照片(修复膜与新生骨组织未发生粘连，便于取出)。
具体实施方式
本发明提供了一种带电防粘连组织修复膜及其制备方法，下面结合附图和具体实施方式对本发明做进一步说明。
实施例1
(1)称取1g P(VDF-TrFE)(65/35，mol％)，加入10mL有机溶剂DMF，搅拌6h使其完全溶解，得到聚合物P(VDF-TrFE)溶液；
(2)取步骤(1)所得溶液于流延机中流延，将所得流延膜置于55℃温度下烘干，使溶剂完全挥发，得到一种组织修复膜材料；
(3)将步骤(2)所得膜材料经过极化处理，极化参数包括：极化电压为15kV，极化距离为15mm，极化温度为25℃，极化时间为30min。
通过以上步骤所得的带电防粘连组织修复膜材料主要成分为聚合物P(VDF-TrFE)，其为薄膜状材料，膜厚为60μm。
实施例2
(1)称取1g聚合物PVDF，加入10mL有机溶剂DMF，搅拌8h使其完全溶解，得到聚合物PVDF溶液；
(2)取步骤(1)所得溶液于流延机中流延，将所得流延膜置于50℃温度下烘干，使溶剂完全挥发，得到一种组织修复膜材料；
(3)将步骤(2)所得膜材料经过极化处理，极化参数包括：极化电压为13kV，极化距离为15mm，极化温度为80℃，极化时间为20min。
通过以上步骤所得的带电防粘连组织修复膜材料主要成分为聚合物PVDF，其为薄膜状材料，膜厚为50μm。
实施例3
(1)称取1g聚合物P(VDF-TrFE)(70/30，mol％)和1g聚合物PLLA，加入20mL有机溶剂DMF，搅拌8h使其完全溶解，得到聚合物P(VDF-TrFE)/PLLA混合溶液；
(2)取步骤(1)所得溶液于流延机中流延，将所得流延膜置于60℃温度下烘干，使溶剂完全挥发，得到一种组织修复膜材料；
(3)将步骤(2)所得膜材料经过极化处理，极化参数包括：极化电压为18kV，极化距离为20mm，极化温度为60℃，极化时间为30min。
通过以上步骤所得的压电活性骨修复复合材料含有聚合物P(VDF-TrFE)和聚合物PLLA，其为薄膜状材料，膜厚为70μm，聚合物P(VDF-TrFE)和聚合物PLLA在复合膜材料中的质量含量各为50％。
实施例4
(1)称取1g聚合物P(VDF-TrFE)(75/25，mol％)和1g天然聚合物壳聚糖，加入20mL有机溶剂DMF，搅拌7h使其完全溶解，得到聚合物P(VDF-TrFE)/壳聚糖混合溶液；
(2)取步骤(1)所得溶液于流延机中流延，将所得流延膜置于50℃ 温度下烘干，使溶剂完全挥发，得到一种组织修复膜材料；
(3)将步骤(2)所得膜材料经过极化处理，极化参数包括：极化电压为15kV，极化距离为12mm，极化温度为40℃，极化时间为40min。
通过以上步骤所得的压电活性骨修复复合材料含有聚合物P(VDF-TrFE)和天然聚合物壳聚糖，其为薄膜状材料，膜厚为80μm，聚合物P(VDF-TrFE)和天然聚合物壳聚糖在复合膜材料中的质量含量各为50％。

Claims (6)

  1. 一种带电防粘连组织修复膜，其特征是所述带电防粘连组织修复膜含有铁电高分子聚偏氟乙烯、铁电高分子聚偏氟-三氟乙烯或两者的复合物。
  2. 根据权利要求1所述的带电防粘连组织修复膜，其特征在于所述带电防粘连组织修复膜为薄膜材料，其厚度为10μm～300μm。
  3. 根据权利要求1所述的带电防粘连组织修复膜，其特征在于所述带电防粘连组织修复膜还含有壳聚糖、透明质酸、胶原、明胶、丝素蛋白中的一种或多种。
  4. 根据权利要求1所述的带电防粘连组织修复膜，其特征在于所述带电防粘连组织修复膜还含有左旋聚乳酸、聚乳酸-羟基乙酸共聚物和聚己内酯中的一种或多种。
  5. 如权利要求1～4任意一项权利要求所述的带电防粘连组织修复膜的制备方法，其特征在于：所述带电防粘连组织修复膜通过有机溶剂溶解以及流延法完成。
  6. 根据权利要求5所述的带电防粘连组织修复膜的制备方法，其特征在于包括如下步骤：
    (1)取聚合物，加入有机溶剂，搅拌3h～8h使其完全溶解，得到聚合物溶液；
    (2)取所述步骤(1)所得溶液于流延机中流延，将所得流延膜置于40℃～100℃温度下烘干，使溶剂完全挥发，得到一种组织修复膜材料，膜厚为1μm～200μm；
    (3)将所述步骤(2)所得膜材料经过极化处理，极化参数包括：极化电压为1kV～30kV，极化距离为0mm～50mm，极化温度为25℃～150℃，极化时间为1min～60min，得到一种带电防粘连组织修复膜。
PCT/CN2018/080426 2017-06-13 2018-03-26 一种带电防粘连组织修复膜及其制备方法 WO2018228018A1 (zh)

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