WO2018214942A1 - 一种冻存细胞试剂盒 - Google Patents

一种冻存细胞试剂盒 Download PDF

Info

Publication number
WO2018214942A1
WO2018214942A1 PCT/CN2018/088245 CN2018088245W WO2018214942A1 WO 2018214942 A1 WO2018214942 A1 WO 2018214942A1 CN 2018088245 W CN2018088245 W CN 2018088245W WO 2018214942 A1 WO2018214942 A1 WO 2018214942A1
Authority
WO
WIPO (PCT)
Prior art keywords
cell
container
kit
cryopreservation
cells
Prior art date
Application number
PCT/CN2018/088245
Other languages
English (en)
French (fr)
Inventor
何佳平
张丽
李超
刘丽萍
刘欣
赵荻骏
张露亿
刘维克多
Original Assignee
西比曼生物科技（上海）有限公司
西比曼生物科技（无锡）有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 西比曼生物科技（上海）有限公司, 西比曼生物科技（无锡）有限公司 filed Critical 西比曼生物科技（上海）有限公司
Publication of WO2018214942A1 publication Critical patent/WO2018214942A1/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the present invention belongs to the field of cell therapy, and in particular, the present invention provides a no-washable intravenously inoculated frozen cell preparation, and simplifies the use of the frozen cell preparation.
  • Fresh cells are typically used for intravenous or topical infusion, or cryopreserved cells are washed and used.
  • Fresh cells usually have an expiration date of less than 8 hours before clinical use, which is not conducive to long-distance transportation, and can not adapt to changes in clinical patients. Therefore, if it is necessary to apply to cells in the therapeutic field, it is bound to involve the process of cryopreservation and resuscitation of cells.
  • cryopreservation in the laboratory, resuscitation washing of frozen cells is usually performed in a clean environment (such as in a sterile room) and requires the operation of a professional biotech technician.
  • a clean environment such as in a sterile room
  • the process of cryopreservation is carried out in a general hospital, unable to provide a sterile room environment, and lack of personnel with corresponding experimental skills, which limits the promotion of cell therapy.
  • the utility model provides a kit suitable for performing cell freezing preparation by using a no-clean cryopreservation solution.
  • the kit can be used for cell resuscitation and dilution by a simple dispensing operation, thereby enabling clinical use. Effectively solve the above problems in the field of cell therapy.
  • a cryopreservation cell kit comprising:
  • physiological saline container wherein the physiological saline container is used for storing physiological saline
  • a cell cryopreservation container for storing the cryopreserved cell preparation
  • the sterile connector includes a catheter portion, and a first lancing device and a second lancing device respectively coupled to both ends of the catheter portion.
  • the saline container has a puncturing portion, and the puncturing portion is punctured by the first puncturing device of the aseptic connector.
  • the cell cryopreservation container has a puncturing portion, and the puncturing portion can be punctured by the second puncturing device of the aseptic connector.
  • the saline container is a sealed bag.
  • the cell cryopreservation container is a sealed bag.
  • the first puncturing device and the second puncturing device are selected from the group consisting of a puncture needle and a Y-shaped blood transfusion device.
  • the puncturing device further has a sterile sleeve.
  • the kit further comprises a water bath device.
  • the water bath device is a constant temperature water bath.
  • Figure 1 is a schematic view of a cell cryopreservation container
  • Figure 2 is a schematic view of a single use sterile connector
  • Figure 3 is a schematic view of a saline container.
  • the present inventors After long-term and intensive research, the present inventors have devised a simple and convenient kit for cryopreservation-resuscitation of cells.
  • the kit simplifies the procedure so that the cryopreservation process routinely performed in a sterile environment can be conveniently performed by a medical professional in a normal laboratory environment. Based on the above findings, the inventors completed the present invention.
  • the utility model provides a frozen cell kit, the kit comprising:
  • physiological saline container wherein the physiological saline container is used for storing physiological saline
  • a cell cryopreservation container for storing the cryopreserved cell preparation
  • the disposable sterile connector includes a catheter portion, and a first lancing device and a second lancing device respectively coupled to both ends of the catheter portion.
  • the physiological saline container and the cell cryopreservation container preferably have a puncturable portion, and the puncturable portion can be pierced by the first puncture device and the second puncture device of the aseptic connector, thereby making the physiological body
  • the saline container and the liquid in the cell cryopreservation container are in communication with each other.
  • the physiological saline container and the cell cryopreservation container are both sealed bags.
  • the saline container and the cell cryopreservation container are of equal or approximate volume to facilitate 1:1 dilution of the cryopreserved cell preparation.
  • both the physiological saline container and the cell cryopreservation container have a gas pressure balance port due to the need to transfer the liquid.
  • the air pressure balance port may be an opening sealed with a semi-permeable membrane or the like, so that the physiological saline or the frozen cell preparation cannot flow out, and the gas can penetrate.
  • the container when the container is a plastic pouch, the container does not have a gas pressure balance port.
  • the sterile connector is preferably a disposable sterile connector to avoid introducing contamination during handling.
  • the first piercing device and the second piercing device are puncture needles.
  • the puncture needle also has a sterile sleeve.
  • freeze cell preparation and “freeze cell preparation” are used interchangeably and refer to a preparation containing therapeutically active cells that is preserved at low temperatures (eg, at -80 ° C, or in a liquid nitrogen environment).
  • the cryopreserved cell preparation includes cells and a cryopreservation solution, and can maintain cell viability under cryopreservation conditions.
  • the cell is an active cell which can be used for cell therapy, and the kind thereof is not particularly limited.
  • the cell is a cell selected from the group consisting of: primary T cell, expansion culture Post-T cells, T cells after gene transduction, stem cells, or a combination thereof.
  • cryopreserved cells can be resuscitated in a conventional non-sterile environment.
  • the resuscitation process comprises the steps of:
  • step (v) Repeat step (iv) twice, pull out the connector, resuscitate the cells and dilute in a saline bag for clinical application.
  • the resuscitation further comprises the steps of: detecting the viability of the cells, and if the viability meets the requirements for use, for clinical use.
  • the constant temperature device is not particularly limited and may be a thermostatic device commonly used in a laboratory (such as a constant temperature water bath).
  • the cell thawing time is from 30 seconds to 3 minutes.
  • the conventional method uses a syringe to draw thawed cells from a cryopreservation bag into a saline bag.
  • the patent uses a single-use sterile connector to connect the saline bag and the cell cryopreservation bag, so that the physiological saline can be repeatedly washed into the cryopreservation bag, thereby reducing the loss in the bag.
  • the frozen storage container of the frozen cell preparation is a frozen storage bag.
  • the cell type is CAR-T cells. Freeze the specifications 2 ⁇ 10 7 /10ml.
  • the components of the frozen cell preparation are shown in the table below.
  • the CAR-T cells were frozen for 3 months with 100 ml, and then thawed in a 37 ° C water bath for 2 minutes, and the thawed cells were diluted into 100 ml of physiological saline using a kit as described in the present invention. The diluted cells were taken for viability assay, tumor antigen response assay and cell proliferation assay.
  • Cell viability changes The diluted cell suspension was tested for viability by trypan blue staining and the results are shown in Figure 1. The results showed that the cells maintained a high survival rate after resuscitation.
  • Proliferative capacity of cells after cryopreservation The cryopreserved cells were placed in complete medium and cultured, and the density of the cells was recorded daily. The medium was supplemented every 2-3 days and a growth curve was drawn (as shown in Figure 2). It can be seen from the figure that after the cryopreservation process, the cells still have better proliferative capacity.
  • the tumor antigen response ability of CAR-T cells before and after cryopreservation cryopreserved cells and tumor cells carrying tumor antigen were co-cultured for 1:1 overnight, and the culture supernatant was collected to detect the amount of IFNr released by CAR-T cells; The ratio of CD137 positive cells expressing CD137 was used to assess tumor antigen response ability.
  • the data before cryopreservation and the data which were further cultured for 7 days after cryopreservation were compared as shown in Fig. 3. The results showed that the cell's tumor antigen response capacity did not decrease significantly after undergoing the cryopreservation-resuscitation process.
  • the frozen cell preparation survival rate after cryopreservation for 3 months is over 90%.
  • the reconstituted frozen CAR-T cell preparation still has value-adding capabilities.
  • the resuscitated frozen CAR-T cell preparation has a reversible decrease in tumor antigen response ability and is restored after continued culture. It shows that after the cryopreservation and recovery process, the original efficacy of fresh cells is preserved, which can be used again.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

提供了一种冻存细胞试剂盒，所述的试剂盒包括：生理盐水容器，所述的生理盐水容器用于存放生理盐水；细胞冻存容器，所述的细胞冻存容器用于存放冻存细胞制剂；无菌连接器，所述的一次性无菌连接器包括一导管部分，和分别连接于导管部分两头的第一穿刺装置和第二穿刺装置。

Description

一种冻存细胞试剂盒 技术领域
本发明属于细胞治疗领域，具体地，本发明提供了一种免洗的可静脉滴注的冰冻细胞制剂，以及使该冰冻细胞制剂使用简单化。
背景技术
细胞治疗应用时，通常采用新鲜的细胞进行静脉或局部输注，或者采用冻存的细胞经过洗涤后进行使用。新鲜的细胞在临床使用之前，一般只有不到8小时的有效期，不利于远程的运输，也不能适应临床病人病情的变化。因此，若需要在治疗领域应用到细胞，势必涉及到对细胞进行冻存和复苏的过程。
在实验室冻存复苏过程中，对冻存的细胞进行复苏洗涤的操作通常需要在洁净的环境中(如无菌室中)进行，且需要专业的生物技术人员进行操作。然而，在实际治疗的过程中，冻存复苏的过程是在普通医院中进行的，无法提供无菌室的环境，也缺乏具有相应实验技巧的人员，这限制了细胞治疗的推广使用。
综上所述，本领域迫切需要一种操作简单，能够在普通实验室环境下进行细胞冻存复苏的方法及相应的装置。
实用新型内容
本实用新型提供了一种适用于用免洗的冻存液进行细胞冰冻制剂的试剂盒，使用所述的试剂盒，能够采用简单配药式的操作进行细胞复苏和稀释既可以进行临床使用，从而有效的解决了细胞治疗领域的上述问题。
本实用新型的第一方面，提供了一种冻存细胞试剂盒，其特征在于，包括：
生理盐水容器，所述的生理盐水容器用于存放生理盐水；
细胞冻存容器，所述的细胞冻存袋用于存放冻存细胞制剂；
无菌连接器，所述的无菌连接器包括一导管部分，和分别连接于导管部分两头的第一穿刺装置和第二穿刺装置。
在另一优选例中，所述的生理盐水容器具有一可穿刺部，且所述的可穿刺部可以被所述无菌连接器的第一穿刺装置穿刺。
在另一优选例中，所述的细胞冻存容器具有一可穿刺部，且所述的可穿刺部可以被所述无菌连接器的第二穿刺装置穿刺。
在另一优选例中，所述的生理盐水容器为密封袋。
在另一优选例中，所述的细胞冻存容器为密封袋。
在另一优选例中，所述的第一穿刺装置和第二穿刺装置选自下组：穿刺针、Y型输血器。
在另一优选例中，所述的穿刺装置还具有一无菌套。
在另一优选例中，所述的试剂盒还包括水浴装置。
在另一优选例中，所述的水浴装置为恒温水浴锅。
应理解，在本实用新型范围内中，本实用新型的上述各技术特征和在下文(如实施例)中具体描述的各技术特征之间都可以互相组合，从而构成新的或优选的技术方案。限于篇幅，在此不再一一累述。
附图说明
图1为细胞冻存容器的示意图；
图2为一次性使用无菌连接器示意图；
图3为生理盐水容器示意图。
具体实施方式
本发明人经过长期而深入的研究，设计了一种操作简便的用于细胞冻存-复苏的试剂盒。所述的试剂盒可以简化操作步骤，使得常规在无菌环境下进行的冻存复苏过程能够在普通实验室环境下，由医疗人员方便地进行。基于上述发现，发明人完成了本实用新型。
冰冻细胞试剂盒
本实用新型提供了一种冰冻细胞试剂盒，所述的试剂盒包括：
生理盐水容器，所述的生理盐水容器用于存放生理盐水；
细胞冻存容器，所述的细胞冻存袋用于存放冻存细胞制剂；
无菌连接器，所述的一次性无菌连接器包括一导管部分，和分别连接于导管部分 两头的第一穿刺装置和第二穿刺装置。
所述的生理盐水容器和细胞冻存容器优选地具有可穿刺部，所述的可穿刺部可以被所述无菌连接器的第一穿刺装置和第二穿刺装置穿刺，从而使所述的生理盐水容器和细胞冻存容器中的液体互相连通。在本实用新型的优选实施方式中，所述的生理盐水容器和细胞冻存容器均为密封袋。
在另一优选例中，所述的生理盐水容器和细胞冻存容器的体积相等或近似，以便于对所述的冻存细胞制剂进行1:1稀释。
由于需要转移液体，因此优选的所述生理盐水容器和细胞冻存容器均具有气压平衡口。所述的气压平衡口可以是封有半透膜或其他类似材料的开口，可以使生理盐水或冻存细胞制剂无法流出，而气体可以穿透。一种优选的实施方式中，当容器为塑料软袋时，所述的容器不具有气压平衡口。
所述的无菌连接器优选为一次性无菌连接器，以避免在操作过程中引入污染。在本发明的优选实施例中，所述的第一穿刺装置和第二穿刺装置为穿刺针。在更优选的实施例中，所述的穿刺针还具有一无菌套。
冻存细胞制剂
如本文所用，术语“冻存细胞制剂”和“冰冻细胞制剂”可互换使用，均指在低温(如-80℃下，或液氮环境中)状态下保存的含有治疗活性细胞的制剂。
所述的冻存细胞制剂中包括细胞和冻存液，且可以在低温冻存条件下保持细胞活性。其中所述的细胞是可以用于细胞治疗的活性细胞，其种类没有特别的限制，本发明的优选实施例中，所述的细胞为选自下组的细胞：原代T细胞、扩增培养后的T细胞、基因转导后的T细胞、干细胞，或其组合。
细胞冻存复苏方法
使用本实用新型的试剂盒，冻存的细胞可以在常规非无菌环境下进行复苏，在本发明的优选实施例中，所述的复苏过程包括步骤：
(i)提供所述的冻存细胞制剂；
(ii)将所述的冻存细胞制剂迅速置于35-40℃的恒温设备中进行复苏，得到复苏后的细胞-冻存液复合物；
(iii)用注射器或一次性使用连接器将所述制剂中的细胞转移至注射载体中。优选的操作方式中，用75％酒精清洁表面后，用一次性使用无菌连接器连接冻存袋和生理盐水袋。
(iv)抬高生理盐水袋，在重力作用下使生理盐水充盈冻存袋。放低生理盐水袋，抬高细胞冻存袋，使细胞悬液在重力作用下流入生理盐水袋中。
(v)重复步骤(iv)两次，拔出连接器，使细胞复苏并稀释在生理盐水袋中，进行临床应用。
在另一优选例中，所述的复苏还包括步骤：对细胞进行活率检测，若活率符合使用要求，则用于临床使用。
所述的恒温设备没有特别的限制，可以为实验室中常用的恒温设备(如恒温水浴锅)。
在另一优选例中，所述的细胞解冻时间为30秒到3分钟。
与现有技术相比，本实用新型的主要优点包括：
1.操作简单：只需要一个恒温水浴锅而不需要其他设备就能完成整个细胞复苏的操作，不需要专业技术人员进行操作。普通人经过简单的培训示范就能进行细胞复苏。
2.细胞损耗低：传统的方法使用注射器将解冻的细胞从冻存袋中抽吸进入生理盐水袋中。本专利采用一次性使用无菌连接器连接生理盐水袋和细胞冻存袋，可以使生理盐水反复冲洗细胞冻存袋，从而降低在袋内的损耗。
3.提升复苏细胞的活率：传统的注射器抽吸细胞时，针头较细较锋利会导致细胞活率下降。操作人员抽吸的速度掌握不当也会导致细胞活率下降。
4.降低污染的风险：临床细胞要求无菌无其他污染，传统的注射器抽吸的方式多次将针头暴露在空气中会增加污染的风险。本专利采用带双头针的一次性使用无菌连接器，大幅度的减小了针头暴露的时间，从而降低污染的风险。
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件，或按照制造厂商所建议的条件。除非另外说明，否则百分比和份数按重量计算。
实施例1
冰冻细胞制剂的冻存容器为冻存袋。细胞类型为CAR-T细胞。冻存规格2×10 7/10ml。冰冻细胞制剂的组分如下表所示。将所述的CAR-T细胞与100ml冻存3个月后，在37℃水浴中解冻2分钟，采用如本实用新型所述的试剂盒将解冻的细胞稀释至100ml生理盐水中。取稀释的细胞进行活率检测、肿瘤抗原应答检测和细胞增殖能力检测。
细胞活率变化：稀释的细胞悬液用台盼蓝染色的方法进行活率检测，结果如图1中所示。结果显示，复苏后细胞保持了较高的存活率。
冻存后细胞的增殖能力：将冻存复苏的细胞置于完全培养基中继续培养，每天记录细胞的密度。每隔2-3天补充培养基，绘制生长曲线(如图2中所示)。从图中可见，在冻存复苏过程后，细胞仍然具有较好的增殖能力。
冻存前后CAR-T细胞的肿瘤抗原应答能力：冻存复苏的细胞和携带肿瘤抗原的肿瘤细胞1:1共培养过夜，收集培养上清液检测CAR-T细胞释放的IFNr量；收集细胞检测CD3阳性细胞表达CD137的比例来评估肿瘤抗原应答能力。冻存前的数据和冻存后继续培养7天的数据作为对比，对比图如图3中所示。结果显示，在经历了冻存-复苏过程后，细胞的肿瘤抗原应答能力没有明显下降。
结论：冻存3个月复苏的冰冻细胞制剂活率达到90％以上。复苏的冰冻CAR-T细胞制剂依然具有增值能力。复苏的冰冻CAR-T细胞制剂，发生肿瘤抗原应答能力的可逆下降，继续培养后得到恢复。说明经过该冻存复苏过程后保存了新鲜细胞原有的效能，可以再临床应用。
在本发明提及的所有文献都在本申请中引用作为参考，就如同每一篇文献被单独引用作为参考那样。此外应理解，在阅读了本发明的上述讲授内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。

Claims (9)

  1. 一种冻存细胞试剂盒，其特征在于，包括：
    生理盐水容器，所述的生理盐水容器用于存放生理盐水；
    细胞冻存容器，所述的细胞冻存袋用于存放冻存细胞制剂；
    无菌连接器，所述的无菌连接器包括一导管部分，和分别连接于导管部分两头的第一穿刺装置和第二穿刺装置。
  2. 如权利要求1所述的试剂盒，其特征在于，所述的生理盐水容器具有一可穿刺部，且所述的可穿刺部可以被所述无菌连接器的第一穿刺装置穿刺。
  3. 如权利要求1所述的试剂盒，其特征在于，所述的细胞冻存容器具有一可穿刺部，且所述的可穿刺部可以被所述无菌连接器的第二穿刺装置穿刺。
  4. 如权利要求1所述的试剂盒，其特征在于，所述的生理盐水容器为密封袋。
  5. 如权利要求1所述的试剂盒，其特征在于，所述的细胞冻存容器为密封袋。
  6. 如权利要求1所述的试剂盒，其特征在于，所述的第一穿刺装置和第二穿刺装置选自下组：穿刺针、Y型输血器。
  7. 如权利要求6所述的试剂盒，其特征在于，所述的穿刺装置还具有一无菌套。
  8. 如权利要求1所述的试剂盒，其特征在于，所述的试剂盒还包括水浴装置。
  9. 如权利要求8所述的试剂盒，其特征在于，所述的水浴装置为恒温水浴锅。
PCT/CN2018/088245 2017-05-24 2018-05-24 一种冻存细胞试剂盒 WO2018214942A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201720586861.1 2017-05-24
CN201720586861.1U CN207121593U (zh) 2017-05-24 2017-05-24 一种冻存细胞试剂盒

Publications (1)

Publication Number Publication Date
WO2018214942A1 true WO2018214942A1 (zh) 2018-11-29

Family

ID=61613909

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2018/088245 WO2018214942A1 (zh) 2017-05-24 2018-05-24 一种冻存细胞试剂盒

Country Status (2)

Country Link
CN (1) CN207121593U (zh)
WO (1) WO2018214942A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN207121593U (zh) * 2017-05-24 2018-03-20 西比曼生物科技（上海）有限公司 一种冻存细胞试剂盒

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202170342U (zh) * 2011-08-02 2012-03-21 江苏省北科生物科技有限公司 一种脐带血分离二联袋
CN102586104A (zh) * 2011-12-23 2012-07-18 北京瑞建天行生物技术有限公司 一种多用途细胞冷冻以及冻存后复苏容器
CN205473713U (zh) * 2016-02-26 2016-08-17 博雅干细胞科技有限公司 适合细胞回输用的干细胞冻存袋
CN207121593U (zh) * 2017-05-24 2018-03-20 西比曼生物科技（上海）有限公司 一种冻存细胞试剂盒

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202170342U (zh) * 2011-08-02 2012-03-21 江苏省北科生物科技有限公司 一种脐带血分离二联袋
CN102586104A (zh) * 2011-12-23 2012-07-18 北京瑞建天行生物技术有限公司 一种多用途细胞冷冻以及冻存后复苏容器
CN205473713U (zh) * 2016-02-26 2016-08-17 博雅干细胞科技有限公司 适合细胞回输用的干细胞冻存袋
CN207121593U (zh) * 2017-05-24 2018-03-20 西比曼生物科技（上海）有限公司 一种冻存细胞试剂盒

Also Published As

Publication number Publication date
CN207121593U (zh) 2018-03-20

Similar Documents

Publication Publication Date Title
KR102350423B1 (ko) 세포 동결 보존 제제 및 세포 소생 방법
JP2009508566A (ja) 臍帯血採血装置および方法
Chasovskyi et al. Arterial switch operation in the first hours of life using autologous umbilical cord blood
WO2018137428A1 (zh) 一种大规模细胞生产和培养的高效率培养系统及应用方法
WO2018214942A1 (zh) 一种冻存细胞试剂盒
JP5328004B2 (ja) 膵島保存方法、膵島保存用容器及び膵島移植用キット
CN105438655A (zh) 用于脂肪移植的颗粒脂肪低温收集装置
JP2013531541A (ja) 閉鎖システムにおいて生体材料を処理する装置及び方法
WO2013088262A1 (en) Device for medical purposes
US6302854B1 (en) Method for collecting fluids
CN115039767A (zh) 一种工序整合分体式生物样本冻融袋及使用方法
WO2021190620A1 (zh) 细胞保存液及其试剂盒
CN204500724U (zh) 一种可实时采集血液或给药的留置针
US10278993B2 (en) Cell-based composition and use thereof for treatment of acute stroke
CN216535527U (zh) 一种便于取用和携带的止血带存放盒
CN203647772U (zh) 一次性使用溶氧器
CN204636385U (zh) 一种设有两个留置针头的留置针装置
CN209220978U (zh) 一种无菌骨髓保护袋
Thirumala et al. A cryopreservation system for direct clinical use of MSC
CN104688244B (zh) 一种可实时采集血液或给药的留置针
CN212347313U (zh) 一种脐带血造血干细胞解冻后清洗装置
CN115521910A (zh) 一种外周血单个核细胞分离方法
CN208160652U (zh) 一种脂肪来源干细胞基质胶移植手术器械工具盒
CN208229970U (zh) 细胞复苏用开放式工作台
CN209360974U (zh) 一种用于实验鼠心脏灌注的固定装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18806023

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18806023

Country of ref document: EP

Kind code of ref document: A1