WO2018192193A1 - Combinaison d'amorces lamp pour détecter six virus respiratoires et son utilisation - Google Patents

Combinaison d'amorces lamp pour détecter six virus respiratoires et son utilisation Download PDF

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Publication number
WO2018192193A1
WO2018192193A1 PCT/CN2017/105455 CN2017105455W WO2018192193A1 WO 2018192193 A1 WO2018192193 A1 WO 2018192193A1 CN 2017105455 W CN2017105455 W CN 2017105455W WO 2018192193 A1 WO2018192193 A1 WO 2018192193A1
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WO
WIPO (PCT)
Prior art keywords
sequence
primer set
dna molecule
seq
same function
Prior art date
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PCT/CN2017/105455
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English (en)
Chinese (zh)
Inventor
郝荣章
王瑞丽
宋宏彬
孔文
李杨
赵荣涛
孙明璇
贾雷立
邱少富
郭旭东
Original Assignee
中国人民解放军疾病预防控制所
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Application filed by 中国人民解放军疾病预防控制所 filed Critical 中国人民解放军疾病预防控制所
Publication of WO2018192193A1 publication Critical patent/WO2018192193A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Definitions

  • the invention belongs to the field of biotechnology, and particularly relates to a LAMP primer combination for detecting six respiratory viruses and an application thereof.
  • Respiratory infections are classified into upper respiratory tract infections and lower respiratory tract infections. About 90% of upper respiratory tract infections are caused by viruses, and bacterial infections are often secondary to viral infections. The disease can occur in all seasons and at any age, and spreads through droplets, mists, or contaminated utensils containing viruses. Whether it is a lower respiratory tract infection or an upper respiratory tract infection, most of it is caused by a virus called a viral respiratory infection.
  • influenza virus belongs to the Orthomyxoviridae family and is classified into influenza A virus (such as influenza A H1N1 influenza virus, influenza A H3N2 influenza virus, H5N1 avian influenza virus, H7N9 avian influenza virus, etc.), influenza B virus and influenza C virus III.
  • influenza A virus such as influenza A H1N1 influenza virus, influenza A H3N2 influenza virus, H5N1 avian influenza virus, H7N9 avian influenza virus, etc.
  • influenza B virus and influenza C virus III.
  • influenza A virus such as influenza A H1N1 influenza virus, influenza A H3N2 influenza virus, H5N1 avian influenza virus, H7N9 avian influenza virus, etc.
  • influenza B virus influenza C virus III.
  • influenza A virus such as influenza A H1N1 influenza virus, influenza A H3N2 influenza virus, H5N1 avian influenza virus, H7N9 avian influenza virus, etc.
  • influenza B virus and influenza C virus III.
  • influenza A virus such as influenza
  • H5N1 avian influenza virus and H7N9 avian influenza virus have been reported in the world.
  • Adenovirus is a non-encapsulated particle with a diameter of 70-90 nm, which consists of 252 shells arranged in a squamous plane. The linear double-stranded DNA molecule is in the capsid.
  • Human adenovirus (HAdV) is a member of the mammalian adenovirus family of the adenoviridae family. Human adenovirus is one of the most important pathogens causing acute respiratory infections in infants and young children.
  • HAdV-3 human adenovirus type 3
  • HAdV-7 human adenovirus type 7
  • the virus is similar, but more with gastrointestinal symptoms.
  • Adenovirus infections have occurred in many countries and regions around the world. In China, serious outbreaks of adenovirus infection occurred in the last century.
  • Loop-mediated isothermal amplification is a chain of denaturation and automatic cycling of nucleic acids under isothermal conditions using a Bst DNA polymerase with strand displacement activity and waterfall nucleic acid amplification. Replace the nucleic acid amplification reaction.
  • LAMP designs 4 special features for 6 regions of the target sequence

Abstract

La présente invention concerne une combinaison d'amorces LAMP pour la détection de six virus respiratoires et son utilisation. La combinaison d'amorces est constituée de molécules d'ADN telles que représentées dans les séquences 1 à 36, et est utilisée pour détecter le virus de la grippe A H1N1, le virus de la grippe A H3N2, le virus de la grippe aviaire H5N1, le virus de la grippe aviaire H7N9, le virus de la grippe B et l'adénovirus humain.
PCT/CN2017/105455 2017-04-18 2017-10-10 Combinaison d'amorces lamp pour détecter six virus respiratoires et son utilisation WO2018192193A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710251770.7 2017-04-18
CN201710251770.7A CN106884012B (zh) 2017-04-18 2017-04-18 用于检测6种呼吸道病毒的lamp引物组合及其应用

Publications (1)

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WO2018192193A1 true WO2018192193A1 (fr) 2018-10-25

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CN (1) CN106884012B (fr)
WO (1) WO2018192193A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106884012B (zh) * 2017-04-18 2020-07-28 中国人民解放军疾病预防控制所 用于检测6种呼吸道病毒的lamp引物组合及其应用
CN108642212A (zh) * 2018-07-02 2018-10-12 上海交通大学医学院附属上海儿童医学中心 环介导等温扩增检测甲型h3n2流感病毒的试剂盒及方法
CN109504803A (zh) * 2018-11-22 2019-03-22 李越希 一种检测7型人腺病毒的rpa方法、其专用引物和探针及用途
CN111172324A (zh) * 2020-02-17 2020-05-19 深圳麦科田生物医疗技术有限公司 基于双重环介导恒温扩增技术检测甲流和乙流病毒的探针、引物、试剂盒及检测方法
CN113136454B (zh) * 2021-04-15 2022-07-12 厦门健康工程与创新研究院 一种用于检测多种流感病毒的多重lamp引物组合及其应用
CN113073151A (zh) * 2021-05-12 2021-07-06 广州普世利华科技有限公司 一种使用酶切探针报告系统的检测腺病毒HAdV的引物探针组合物及其试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015048098A1 (fr) * 2013-09-24 2015-04-02 Washington University Procédé de diagnostic pour une maladie infectieuse utilisant l'expression de gène endogène
CN106884012A (zh) * 2017-04-18 2017-06-23 中国人民解放军疾病预防控制所 用于检测6种呼吸道病毒的lamp引物组合及其应用

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CN104278024B (zh) * 2013-07-05 2016-12-28 中国人民解放军军事医学科学院微生物流行病研究所 用于鉴定人类腺病毒55型的引物组合物以及它们的应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015048098A1 (fr) * 2013-09-24 2015-04-02 Washington University Procédé de diagnostic pour une maladie infectieuse utilisant l'expression de gène endogène
CN106884012A (zh) * 2017-04-18 2017-06-23 中国人民解放军疾病预防控制所 用于检测6种呼吸道病毒的lamp引物组合及其应用

Non-Patent Citations (1)

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Title
LIU, FANG ET AL.: "Surveillance Analysis of Respiratory Viruses of Entry Pilgrims in Gansu Port During 2014", CHINESE JOURNAL OF FRONTIER HEALTH AND QUARANTINE, vol. 38, no. 3, 30 June 2015 (2015-06-30) *

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CN106884012B (zh) 2020-07-28
CN106884012A (zh) 2017-06-23

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