WO2018171009A1

WO2018171009A1 - Flat-cover type fungus culture device

Info

Publication number: WO2018171009A1
Application number: PCT/CN2017/083398
Authority: WO
Inventors: 于津
Original assignee: 珠海美华医疗科技有限公司
Priority date: 2017-03-22
Filing date: 2017-05-08
Publication date: 2018-09-27

Abstract

A flat-cover type fungus culture device, comprising a glass slide (1) and a culture dish (2), wherein the glass slide (1) is disposed above the culture dish (2); the glass slide (1) and the culture dish (2) are of an integrated structure and form a fungus culture chamber (3); a flat cover (5) is provided at the upper part of the culture chamber (3); the upper surface of the flat cover (5) is flush with the glass slide (1); the sidewall of the culture dish (2) is provided with a through hole (4) for entering and inoculating; and the through hole (4) is communicated with the fungus culture chamber (3).

Description

Flat-cover fungus culture device

Technical field

The present invention relates to a microbial experimental device, and more particularly to a flat lid fungus culture device.

Background technique

In the fungal experiment, most of the morphological changes were observed by small culture method. The fungi were usually cultured in a solid medium in a culture dish. After a certain period of time, the fungal culture device was transferred to a microscope for morphological identification to observe and understand. The growth and culture of fungi. However, when the fungal culture device is observed under a microscope, the clarity of the observed fungus is affected by the relationship between the thickness of the flat cover of the culture device and the material, so in order to observe the fungus more clearly, a high-magnification microscope has to be used. The morphology of the fungus can be clearly observed, which increases the cost of use. Therefore, there is an urgent need in the market for a device to overcome these drawbacks, thereby reducing the cost of use.

Summary of the invention

In order to overcome the above deficiencies of the prior art, the present invention provides a flat-cover fungus culture apparatus which can observe the growth of fungal culture under high magnification and oil microscope, and reduce the use cost.

The technical solution adopted by the present invention to solve the technical problem thereof is: a flat-cover fungus culture device comprising a slide glass and a culture dish, characterized in that the slide glass is disposed above the culture dish, the slide glass And the culture dish is a monolithic structure and forms a fungus culture cavity, a flat cover is arranged on the upper part of the culture cavity, the upper surface of the flat cover is flush with the slide glass, and the side wall of the culture dish is provided with a passage for extending into the inoculation a hole that communicates with the fungal culture chamber.

As a modification of the above technical solution, the axial direction of the through hole is perpendicular to the longitudinal direction of the slide glass.

As an improvement of the above technical solution, the petri dish has a circular cross section.

As a modification of the above technical solution, the cover glass has a circular shape and a diameter larger or smaller than the diameter of the culture dish.

As a modification of the above technical solution, the cover glass is ultrasonically welded to the upper portion of the culture dish.

As an improvement of the above technical solution, the slide glass is provided with a frosted area on both sides.

As a modification of the above technical solution, the slide glass and the culture dish are each made of a light transmissive polystyrene material.

As an improvement of the above technical solution, the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.

As an improvement of the above technical solution, the four corners of the slide have a chamfer.

As an improvement of the above technical solution, the culture dish has a diameter of 15 to 25 mm and a height of 2 to 5 mm, and the through hole has an inner diameter of 1.0 to 4.0 mm.

As an improvement of the above technical solution, one side of the slide glass is further provided with a trademark area marked with a trademark.

Compared to the prior art, the flat-cover fungal culture apparatus of the present invention has improved the flat cover of the culture dish to use a glass piece or other material having a thinner thickness. When the culture dish is placed under a microscope, the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that under the same multiple microscope, the morphology of the fungus can be observed more clearly, thereby achieving the use of high magnification and oil mirror. The microscope can perform observations, which in turn reduces procurement, labor, and material costs, and has significant economic benefits.

DRAWINGS

1 is a schematic structural view of an embodiment of the present invention;

Figure 2 is a front elevational view of an embodiment of the present invention.

detailed description

The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the embodiments in the present application and the features in the embodiments may be combined with each other without conflict. The contents of the drawings and the ratios of the drawings are only intended to be illustrative, and are not intended to limit the scope of the invention.

As shown in FIG. 1 , the present invention provides a flat lid fungus culture apparatus comprising a slide glass 1 , a culture dish 2 is disposed under the slide glass 1 , and the slide glass 1 and the culture dish 2 are integrally formed. The fungus culture chamber 3 is formed, and the upper portion of the culture chamber 3 is provided with a flat cover 5 and the upper surface of the flat cover 5 is flush with the slide glass 1. The side wall of the culture dish 2 is provided with a through hole 4 for extending into the inoculation, and the through hole 4 It is in communication with the fungal culture chamber 3. Wherein, the axial direction of the through hole 4 is perpendicular to the longitudinal direction of the slide glass 1, and the through hole 4 is used for inoculating the needle to inject the fungus into the solid medium for inoculation, and the fungus culture chamber 3 is small because of the small pore diameter. The substance is not volatile. The slide 1 and the culture dish 2 are both made of a light transmissive polystyrene material.

As shown in Fig. 1, in a preferred embodiment of the present invention, a sanding zone 7 is provided on both sides of the slide 1 to facilitate marking, and one side of the slide 1 is also provided with a trademark area for marking a trademark. 8, the size and location of the area depends on the needs.

As shown in FIG. 2, according to another preferred embodiment of the present invention, the diameter of the flat cover 5 of the culture dish 2 is larger than the inner diameter of the culture dish 2 but smaller than the outer diameter of the culture dish 2, so that the flat cover 5 is fixedly fixed by ultrasonic waves. It is welded over the petri dish 2, and the upper surface of the flat cover 5 is flush with the slide glass 1. The flat cover 5 is made of glass and is a piece of glass. In another embodiment, the flat cover 5 can also be replaced with a cover glass, and the joint 6 of the culture dish 2 and the flat cover 5 can be sealed by paraffin to meet different requirements and closer to the conventional display. Micro observation mode.

In a preferred embodiment of the present invention, the cross section of the culture dish 2 may be a circular or elliptical or regular polygon. In the illustrated embodiment, the culture dish 2 is circular in cross section, and the slide 1 is rectangular. The slide 1 has a length of 50 to 100 mm and a width of 20 to 30 mm, the diameter of the culture dish 2 is 15 to 25 mm, the height is 2 to 5 mm, and the inner diameter of the through hole 4 is 1.0 to 4.0 mm. Specifically, In this embodiment, the length of the slide 1 is 75.9 mm, the width is 25.2 mm, the diameter of the culture dish 2 is 23.1 mm, the height is 4.35 mm, and the inner diameter of the through hole 4 is 2.7 mm, which facilitates the placement of the culture device. Observed under the microscope, no additional fungus is placed under the microscope to prevent laboratory contamination.

In contrast to the prior art, the present invention provides a flat-cover fungal culture apparatus that improves the flat lid of a petri dish to use a glass sheet or other material having a thinner thickness. When the culture dish is placed under a microscope, the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that under the same multiple microscope, the morphology of the fungus can be observed more clearly, thereby achieving the use of high power and oil. The microscope microscope can perform observations, which reduces the cost of procurement, labor and materials, and has significant economic benefits.

The above is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiment, and as long as it achieves the technical effects of the present invention by the same means, it should fall within the scope of protection of the present invention. Various modifications and changes may be made to the technical solutions and/or embodiments within the scope of the invention.

Claims

A flat-cover fungus culture apparatus comprising a slide glass (1) and a culture dish (2), characterized in that the slide glass (1) is disposed above the culture dish (2), the slide glass ( 1) and the culture dish (2) is an integrally formed structure and forms a fungal culture chamber (3), a flat cover (5) is disposed on the upper portion of the culture chamber (3), and the upper surface of the flat cover (5) and the slide (1) Leveling, the side wall of the culture dish (2) is provided with a through hole (4) for extending into the inoculation, and the through hole (4) is in communication with the fungal culture chamber (3).
The flat-cover fungus culture apparatus according to claim 1, wherein the axial direction of the through hole (4) is perpendicular to the longitudinal direction of the slide glass (1).
The flat-cover fungus culture apparatus according to claim 1, wherein the culture dish (2) has a circular cross section.
The flat-cover fungus culture apparatus according to claim 1, characterized in that the flat cover (5) has a circular shape with a diameter equal to the outer diameter of the culture dish (2).
A flat-cover fungus culture apparatus according to claim 1 or 4, characterized in that the flat cover (5) is ultrasonically welded to the upper portion of the culture dish (2).
The flat-cover fungus culture apparatus according to claim 1, characterized in that the glass slide (1) is provided with a frosted zone (7) on both sides.
The flat-cover fungus culture apparatus according to claim 1, wherein the slide glass (1) and the culture dish (2) are each made of a light-transmitting polystyrene material.
The flat-cover fungus culture apparatus according to claim 7, wherein the slide glass (1) has a length of 50 to 100 mm and a width of 20 to 30 mm.
The flat-cover fungus culture apparatus according to claim 7, wherein the four corners of the slide glass (1) are chamfered.
The flat-cover fungus culture apparatus according to claim 1, wherein one side of the slide glass (1) is provided with a trademarked trademark area (8).