WO2018168976A1 - Liquid composition for bacteriostasis or sterilization - Google Patents

Liquid composition for bacteriostasis or sterilization Download PDF

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Publication number
WO2018168976A1
WO2018168976A1 PCT/JP2018/010105 JP2018010105W WO2018168976A1 WO 2018168976 A1 WO2018168976 A1 WO 2018168976A1 JP 2018010105 W JP2018010105 W JP 2018010105W WO 2018168976 A1 WO2018168976 A1 WO 2018168976A1
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WIPO (PCT)
Prior art keywords
acid monoglyceride
liquid composition
composition
ultraphosphate
mass
Prior art date
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PCT/JP2018/010105
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French (fr)
Japanese (ja)
Inventor
治子 奥田
亘 石田
俊之 宮崎
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株式会社日清製粉グループ本社
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Application filed by 株式会社日清製粉グループ本社 filed Critical 株式会社日清製粉グループ本社
Priority to JP2019506244A priority Critical patent/JP7058257B2/en
Publication of WO2018168976A1 publication Critical patent/WO2018168976A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3481—Organic compounds containing oxygen
    • A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
    • A23L3/3517—Carboxylic acid esters

Definitions

  • the present invention relates to a bactericidal or bacteriostatic composition.
  • Patent Document 1 describes a food disinfectant composition containing a fatty acid monoester, an acid or chelating agent, a food grade surfactant and a medium.
  • Patent Document 2 describes a food preservative comprising lysozyme, sodium hexametaphosphate, and capric acid monoglyceride.
  • Patent Document 3 describes a food preservative characterized by using glycine, sodium acetate, lysozyme, and polymerized phosphate in combination with caprylic acid monoglyceride and / or capric acid monoglyceride.
  • Patent Document 4 discloses an antibacterial substance derived from horseradish, an organic acid or a salt thereof, an amino acid, a lower fatty acid ester, a sugar ester, a vitamin B1 ester, a polymerized phosphate, phytic acid, ethanol and the like. Or the preservative for foods containing 2 or more types is described.
  • Patent Document 5 describes a liquid food preservative in which lauric acid, decaglycerin fatty acid monoester, sucrose fatty acid monoester, and organic acid are mixed and dissolved in ethanol.
  • compositions for sterilization or bacteriostasis of food or the equipment that handles it or the environment in which it exists not only the sterilization or bacteriostatic effect is important, but also the effect on safety and food flavor. It cannot be ignored.
  • compositions that have been used in the field of food to control the growth of microorganisms meet the safety standards, but are difficult to form a homogeneous liquid with low water solubility. Often difficult to use.
  • emulsifiers and acids are often used in conventional food preservative compositions, but in general emulsifiers have a strong bitter taste, and acids have a sour taste. If these remain in equipment after washing and sterilization, the flavor of food may be affected.
  • the inventors of the present invention have achieved high sterilization or bacteriostasis by adding ultraphosphate to a composition containing a predetermined amount of caprylic acid monoglyceride and / or capric acid monoglyceride and adjusting its pH to 3.5 or less. It has been found that a liquid composition with little bitterness can be obtained while having an action. Furthermore, the present inventors have found that the growth of microorganisms in the material can be suppressed for a long time by spraying or applying the liquid composition to the material.
  • the present invention contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more.
  • a liquid composition for sterilization or bacteriostasis that has a pH of 3.5 or less.
  • the present invention also includes at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more.
  • the present invention includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition, and the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride
  • the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride
  • a bactericidal or bacteriostatic method comprising a seed and an ultraphosphate, wherein the total concentration of caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more and pH 3.5 or less.
  • the present invention includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition, and the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride Provided with a method for imparting antibacterial properties to a subject, comprising seeds and ultraphosphate, wherein the total concentration of caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more and pH 3.5 or less To do.
  • the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more.
  • the use of a liquid composition having a pH of 3.5 or lower for sterilization or bacteriostasis is provided.
  • the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more.
  • the use of a liquid composition having a pH of 3.5 or less for imparting antibacterial properties to a subject is provided.
  • the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more.
  • the use of a liquid composition having a pH of 3.5 or less for the production of a liquid composition for bactericidal or bacteriostatic treatment is provided.
  • the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more.
  • the use of a liquid composition having a pH of 3.5 or less for the production of a liquid composition for imparting antibacterial properties to a subject is provided.
  • the liquid composition for bactericidal or bacteriostatic use of the present invention is liquid, it is easy to use and has a high bactericidal or bacteriostatic effect but has little bitterness and hardly affects the flavor of food. It is effective for sterilization or bacteriostasis of the equipment that handles or the environment where it exists. Moreover, when the liquid composition of the present invention is applied to a material, it can sterilize or bacterioculate microorganisms attached to the material, and can continuously suppress the growth of microorganisms in the material for a long time thereafter. it can. Therefore, the present invention is effective in the field where microbial growth suppression is constantly desired, for example, the field of food production, processing, storage and the like.
  • the present invention provides a bactericidal or bacteriostatic composition.
  • the bactericidal or bacteriostatic composition of the present invention contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride, and an ultraphosphate.
  • caprylic acid monoglyceride and capric acid monoglyceride have been conventionally applied to foods as emulsifiers and are commercially available.
  • caprylic acid monoglyceride is Sunsoft (registered trademark) 700p-2 (Taiyo Chemical Co., Ltd.), Poem M-100 (Riken Vitamin Co., Ltd.), and capric acid monoglyceride is Sunsoft (registered trademark) 760 (Taiyo Chemical). Co., Ltd.) and Poem M-200 (RIKEN Vitamin Co., Ltd.).
  • caprylic acid monoglyceride or capric acid monoglyceride may be used, or may be used in combination.
  • the total concentration of caprylic acid monoglyceride and capric acid monoglyceride in the composition of the present invention is preferably 0.05% by mass or more, more preferably 0.1% by mass or more in the total amount of the composition. When this concentration is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties.
  • the total concentration of caprylic acid monoglyceride and capric acid monoglyceride in the composition of the present invention is preferably 10.0% by mass or less, more preferably 2.0% or less in the total amount of the composition.
  • an example of the range of the total concentration of caprylic acid monoglyceride and capric acid monoglyceride in the composition of the present invention is preferably 0.05 to 10.0% by mass, more preferably 0.05 to 10.0% by mass in the total amount of the composition. 2.0% by mass, more preferably 0.05 to 1.0% by mass, still more preferably 0.05 to 0.5% by mass, and still more preferably 0.1 to 0.5% by mass.
  • Ultraphosphoric acid is also called acidic hexametaphosphoric acid.
  • sodium ultraphosphate is preferred.
  • sodium ultraphosphate has been applied to foods and the like as a binder, a pH adjuster and the like.
  • Ultraphosphate is commercially available, for example, sold under trade names such as Ultrapolyphos (Polyphos Chemical Laboratory Co., Ltd.) and Ultraporin (Taihei Chemical Sangyo Co., Ltd.).
  • the concentration of ultraphosphate in the composition of the present invention may vary depending on the concentration of caprylic acid monoglyceride or capric acid monoglyceride, but is preferably 0.015% by mass or more, more preferably 0.018% in the total amount of the composition. It is at least mass%, more preferably at least 0.02 mass%. When the concentration of ultraphosphate is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, the concentration of the ultraphosphate in the composition of the present invention is preferably 3.8% by mass or less, more preferably 2.5% by mass or less, and further preferably 1.0% by mass or less in the total amount of the composition. It is.
  • the total amount of the composition is preferably 0.015 to 3.8% by mass, more preferably 0.018 to 2.5% by mass. %, More preferably 0.02 to 1.0% by mass.
  • Caprylic acid monoglyceride, capric acid monoglyceride, and ultraphosphate that can be included in the composition for bactericidal or bacteriostatic treatment of the present invention are all substances that are acceptable as food additives. Therefore, the composition of the present invention has higher safety for the human body than general cleaning agents and disinfectants. Moreover, since the composition of the present invention does not have a strong bitter taste like conventional food preservatives and bactericidal components, even if it is taken together with food, it hardly affects the flavor of the food. Therefore, the composition of the present invention is useful for application in food-related fields, for example, sterilization or bacteriostasis of food, or the equipment in which it is handled or the environment in which it exists.
  • the composition of the present invention comprises caprylic acid monoglyceride, capric acid monoglyceride, other components other than ultraphosphate, for example, phosphoric acid other than ultraphosphoric acid (polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid), phytic acid, citric acid , Gluconic acid, fumaric acid, maleic acid, malonic acid, tartaric acid, succinic acid, malic acid, adipic acid, hydrochloric acid, and other organic and inorganic acids other than ultraphosphoric acid, and their salts
  • You may contain the fatty acid ester other than the pH adjuster which can be used for a foodstuff, caprylic acid monoglyceride, capric acid monoglyceride, etc. at least.
  • the types and contents of these other components are limited to such an extent that the bactericidal or bacteriostatic properties necessary for the composition of the present invention are not lost.
  • the composition of the present invention preferably does not contain a fatty acid ester having a chain length of C11 or more.
  • the content of fatty acid esters other than caprylic monoglyceride and capric monoglyceride is preferably 5% by mass or less. More preferably, content of the said other component in the composition of this invention is 5 mass% or less.
  • the composition of the present invention may further contain alcohols.
  • alcohols used in the composition of the present invention include alcohols that can be used in foods such as ethanol. By adding alcohols, the solubility of caprylic acid monoglyceride or capric acid monoglyceride is improved, and the quality of the composition of the present invention is stabilized.
  • the content of alcohols in the composition of the present invention may be equal to or less than the remainder of the caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components described above, preferably the caprylic acid monoglyceride, capric acid monoglyceride, The balance of ultraphosphate and other components, more preferably 20 to 60% by mass in the total amount of the composition.
  • the composition of the present invention may further contain an aqueous solvent.
  • a preferable aqueous solvent includes, for example, water.
  • the content of the aqueous solvent in the composition of the present invention may be equal to or less than the remainder of the caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components described above, and preferably the caprylic acid monoglyceride and capric acid monoglyceride. , Ultraphosphate, other components and the balance of alcohols, more preferably 20% by mass or more.
  • composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in the aqueous solvent.
  • a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in the above alcohols.
  • a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in a mixed solvent of the above alcohols and an aqueous solvent. It is.
  • a preferred example of the composition of the present invention is an aqueous ethanol solution in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in a mixed solvent of ethanol / water.
  • the mixed solvent of ethanol / water is preferably a 20 to 60% by mass aqueous ethanol solution.
  • the content of the mixed solvent of the alcohols and the aqueous solvent or the mixed solvent of the ethanol / water in the composition of the present invention is the above-described caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate, and the remainder of the other components. If it is.
  • the pH of the composition of the present invention is preferably 3.5 or less, more preferably 3.0 or less.
  • the pH of the composition of the present invention is preferably 1.5 or more.
  • examples of the pH range of the composition of the present invention include preferably 1.5 to 3.5, more preferably 1.5 to 3.0.
  • the pH of the composition of the present invention can be adjusted mainly by the addition of ultraphosphate.
  • an acid other than ultraphosphate or a pH adjuster usable for food may be used.
  • the composition of the present invention has an excellent bactericidal or bacteriostatic action, and can be used for bactericidal or bacteriostatic of various substances.
  • sterilization refers to killing microorganisms (but not limited to complete killing)
  • bacteriostasis refers to inhibiting the growth of microorganisms. Therefore, in the subject to which the sterilizing or bacteriostatic composition of the present invention is applied, the growth of microorganisms is suppressed or the number of microorganisms is reduced.
  • the kind of microorganisms sterilized or bacteriostatic by the composition of the present invention is not particularly limited. Yeast, molds of the genus Cladosporium, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa L. , Etc.
  • acid-resistant bacteria such as lactic acid bacteria have conventionally been difficult to remove because they survive even in the low pH range where normal microorganisms are inhibited from growing, but these are also sterilized or bacteriostatic by the composition of the present invention. Can do.
  • the composition of the present invention has high utility value because it is also useful for microbial species that have been difficult to remove in the past, such as acid-resistant bacteria.
  • the composition of the present invention can be used for sterilization or bacteriostatic of various materials.
  • the application target of the composition of the present invention is not particularly limited as long as contact with the composition of the present invention is allowed.
  • Preferable examples of the application target of the composition of the present invention include foods, equipment for handling foods, and environments where foods are present.
  • Equipment that handles the food includes equipment that may come into contact with food in the process of production, processing, distribution, storage, cooking, etc., for example, equipment on the production line of a food production or processing plant, storage or transportation of food Containers, cooking utensils, cooking tables, workers' gloves and apron, work clothes, etc.
  • the environment in which food exists is the environment in which food is handled, for example, the environment in which food is manufactured, processed, distributed, stored, cooked, etc., and more specifically, the food manufacturing or processing factory, food storage, cooking place, etc. Walls, floors, ceilings, pillars, doors, windows, ventilation holes.
  • the sterilization or bacteriostasis of an object by the sterilization or bacteriostatic composition of the present invention can be performed by bringing the composition of the present invention into contact with the object.
  • the composition for bactericidal or bacteriostatic of the present invention is liquid, it can be easily applied to a subject to be bactericidal or bacteriostatic.
  • the liquid composition of the present invention may be mixed in a substance to be sterilized or bacteriostatic (for example, food).
  • the liquid composition of the present invention may be sprayed or applied to a substance to be sterilized or bacteriostatic, or the target substance may be immersed in the liquid composition of the present invention.
  • the liquid composition of the present invention is sprayed or applied to a subject to be sterilized or bacteriostatic.
  • Objects that come into contact with the liquid composition of the present invention are sterilized or bacteriostatic.
  • the composition of the present invention is brought into contact with the subject by spraying, applying, or immersing the composition of the present invention on the subject to be sterilized or bacteriostatic.
  • the object brought into contact with the composition of the present invention may be dried.
  • the drying method include, but are not particularly limited to, drying by standing, air drying, and drying by a dryer.
  • volatile components contained in the composition of the present invention such as an aqueous solvent and ethanol, are volatilized and removed from the target. Even after the removal of the volatile components, the bactericidal or bacteriostatic action continues in the subject to which the composition of the present invention is applied. Therefore, in this embodiment, the composition of the present invention not only exhibits sterilization or bacteriostatic activity against a subject in a liquid state, but also suppresses the growth of microorganisms in the subject even in a dry matter state.
  • composition of the present invention may be a liquid composition for imparting antibacterial properties to a subject.
  • the antibacterial property is imparted to the subject by adhering the composition of the present invention to the subject to be imparted with the antibacterial property by spraying, coating, dipping, or the like. If necessary or preferably, the object to which the composition of the present invention is further adhered is dried.
  • antibacterial of a subject refers to the property of the subject that microorganisms attached to or invaded the surface of the subject can be sterilized or bacteriostatic. This aspect is very useful for an environment where it is desired to constantly prevent microbial growth, or for materials and equipment used therein, for example, an environment where food is handled or food handling equipment used there.
  • the composition of the present invention is used at a dose of about 0.1 to 5.0 mL per 100 cm 2 area of the subject, whether used for sterilization or bacteriostasis of the subject, or for imparting antibacterial properties to the subject. Preferably, it is used at a dose of about 0.5 to 1.0 mL per 100 cm 2 area of the subject. Preferably, this dose of the composition is evenly applied to the subject, preferably by spraying or application.
  • Test example 1 Capillic acid monoglyceride or capric acid monoglyceride and purified water were mixed at room temperature, and various acids were added thereto to adjust the pH, thereby preparing liquid compositions having the compositions shown in Table 2. The taste (bitterness) of the obtained composition was evaluated based on the same criteria as in the reference example.
  • Test example 2 At room temperature, a solution is prepared by mixing caprylic acid monoglyceride or capric acid monoglyceride, ethanol and sterilized purified water in a ratio of 1: 2: 2, to which ultraphosphate or other phosphate, and the remainder Ethanol and sterilized purified water were added to obtain liquid compositions (solutions) shown in Tables 4-5. About each obtained liquid composition, the bactericidal or bacteriostatic characteristic was evaluated in the following procedure.
  • Bactericidal or bacteriostatic characteristics evaluation Based on the number of bacteria measured in (2), the bactericidal or bacteriostatic characteristics of each composition were evaluated according to the following evaluation criteria.
  • the number of bacteria decreased to 1/10 or less of the first time and more than 1/100 of the first time.
  • D The number of bacteria is more than 1/10 of the first
  • compositions containing phosphates other than ultraphosphates could not sustain bactericidal or bacteriostatic effects.
  • Test example 3 In the same procedure as in Test Example 2, except that the concentration of caprylic acid monoglyceride or capric acid monoglyceride was changed, liquid compositions shown in Tables 6 to 7 were prepared. About the obtained composition, taste (bitter taste) was evaluated in the same procedure as the reference example, and bactericidal or bacteriostatic properties were evaluated in the same procedure as in Test Example 2. The results are shown in Tables 6-7. The composition containing 0.05% by mass or more of caprylic acid monoglyceride or capric acid monoglyceride had no bitter taste and had a continuous bactericidal or bacteriostatic effect.
  • Test example 4 A liquid composition shown in Tables 8 to 9 was prepared in the same procedure as in Test Example 2 except that the concentration of ultraphosphate was changed. Bacteriostatic properties were evaluated. The results are shown in Tables 8-9. The composition with a low concentration of ultraphosphate had a bitter taste and did not show a continuous bactericidal or bacteriostatic effect.
  • Test Example 5 C8-C12 fatty acid monoglyceride and purified water were mixed at room temperature, and the pH was adjusted by adding sodium ultraphosphate to prepare a liquid composition having the composition shown in Table 10.
  • the taste (bitterness) and liquid homogeneity (solubility of the components) of the obtained composition were evaluated. About taste property, it evaluated on the basis similar to a reference example. About the homogeneity of the liquid, the presence or absence of precipitation of components (white turbidity or precipitation) was visually confirmed for the composition immediately after preparation or after standing overnight in a refrigerator.
  • Test Example 6 The sterilization or bacteriostatic action of the liquid composition on various materials was investigated.
  • a liquid composition was prepared in the same procedure as in Test Example 2 (caprylic acid monoglyceride 0.15%, ultraphosphoric acid Na 0.40%, ethanol 40.00%, water residue; unit is mass%; pH 2.4) ).
  • a sample bacterial solution was prepared using four types of bacteria in the same procedure as in Test Example 2.
  • target materials metal (stainless steel plate), rubber (rubber gloves), and plastic (guard cover) were prepared. The stainless steel plate and watch cover were cut into 10 cm squares. The rubber gloves were stretched on a 10 cm square plastic plate so as not to be wrinkled and attached with double-sided tape, and the remaining part was cut and used.
  • the number of bacteria was measured according to the following procedure for each material.
  • Each target material was divided into 3 groups.
  • 0.5 mL of the liquid composition was evenly applied on the target material with a large rod, then 0.1 mL of the sample bacterial solution was inoculated, and spread with mixing with a large rod for 40 seconds ( 0h group).
  • 0.5 mL or 1.0 mL of the liquid composition was evenly applied onto the target material with a large stick and then air-dried in a safety cabinet for 18 hours to completely evaporate moisture and ethanol. .
  • the initial bacterial count of the sample bacterial solution was adjusted to 10 5 to 10 6 cfu / mL.
  • the target material to which the sample bacterial solution was applied was allowed to stand for 5 minutes, then placed in a sterile pouch, added with 10 mL of sterilized water from above, heat-sealed, and gently shaken up and down about 5 times.
  • the pouch was opened, the liquid inside was collected, and the number of bacteria was measured on an MRS agar medium.

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Abstract

Provided is a composition which exhibits a strong sterilizing or bacteriostatic effect as a liquid, while not being very bitter or sour. This liquid composition for bacteriostasis or sterilization contains one or more types selected from caprylic acid monoglyceride and capric acid monoglyceride, also contains an ultraphosphate, exhibits a total caprylic acid monoglyceride and capric acid monoglyceride concentration of 0.05 mass% or more, and exhibits a pH of 3.5 or less.

Description

殺菌又は静菌用液状組成物Liquid composition for bactericidal or bacteriostatic
　本発明は、殺菌又は静菌用組成物に関する。 The present invention relates to a bactericidal or bacteriostatic composition.
　従来、食品製造においては、微生物の増殖による食品の腐敗や変質を防ぐための組成物が使用されている。特許文献１には、脂肪酸モノエステル、酸またはキレート化剤、食品グレードの界面活性剤及び媒体を含有する食品用殺菌剤組成物が記載されている。特許文献２には、リゾチーム、ヘキサメタリン酸ソーダ及びカプリン酸モノグリセリドからなる食品用保存料が記載されている。特許文献３には、カプリル酸モノグリセリド及び／又はカプリン酸モノグリセリドに、グリシン、酢酸ナトリウム、リゾチーム、ならびに重合リン酸塩を併用することを特徴とする食品用保存剤が記載されている。特許文献４には、ホースラディッシュ由来の抗菌性物質と、有機酸又はその塩、アミノ酸、低級脂肪酸エステル、シュガーエステル、ビタミンＢ１エステル、重合リン酸塩、フィチン酸、エタノール等から選択される１種又は２種以上とを含有する食品用保存剤が記載されている。特許文献５には、エタノールに、ラウリン酸、デカグリセリン脂肪酸モノエステル、ショ糖脂肪酸モノエステル及び有機酸を混合溶解させた液状の食品保存剤が記載されている。 Conventionally, in food production, compositions for preventing food spoilage and alteration due to the growth of microorganisms have been used. Patent Document 1 describes a food disinfectant composition containing a fatty acid monoester, an acid or chelating agent, a food grade surfactant and a medium. Patent Document 2 describes a food preservative comprising lysozyme, sodium hexametaphosphate, and capric acid monoglyceride. Patent Document 3 describes a food preservative characterized by using glycine, sodium acetate, lysozyme, and polymerized phosphate in combination with caprylic acid monoglyceride and / or capric acid monoglyceride. Patent Document 4 discloses an antibacterial substance derived from horseradish, an organic acid or a salt thereof, an amino acid, a lower fatty acid ester, a sugar ester, a vitamin B1 ester, a polymerized phosphate, phytic acid, ethanol and the like. Or the preservative for foods containing 2 or more types is described. Patent Document 5 describes a liquid food preservative in which lauric acid, decaglycerin fatty acid monoester, sucrose fatty acid monoester, and organic acid are mixed and dissolved in ethanol.
特開２００５－２９６０２１号公報Japanese Patent Laid-Open No. 2005-296021 特開昭５９－１５１８７５号公報JP 59-151875 A 特開平６－２１７７４９号公報JP-A-6-221749 特開平６－１５３８８２号公報JP-A-6-153882 特開２００５－０８０６１７号公報JP 2005-080617 A
　食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌のための組成物においては、その殺菌又は静菌効果が重要であるだけでなく、安全性や食品の風味に与える影響も無視できない。一方で、食品分野で微生物の増殖制御に従来使用されている組成物は、安全性の基準は満たすが、水溶性が低く均質な液状とすることが難しいため、機器等の洗浄や殺菌には使いづらいことが多い。さらに、従来の食品保存料組成物には、乳化剤や酸がしばしば使用されているが、一般に乳化剤は強い苦味を有しており、また酸には酸味がある。これらが洗浄や殺菌後に機器等に残留すると、食品の風味に影響するおそれがある。反面、食品の風味を考慮して組成物の使用量を制限すると、充分な殺菌又は静菌効果が得られなくなるおそれがある。したがって、均質な液状を呈し、かつ高い殺菌又は静菌効果を有しながらも苦味や酸味が少なく食品の風味に影響を与えにくい、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有用な組成物が求められている。 In the composition for sterilization or bacteriostasis of food or the equipment that handles it or the environment in which it exists, not only the sterilization or bacteriostatic effect is important, but also the effect on safety and food flavor. It cannot be ignored. On the other hand, compositions that have been used in the field of food to control the growth of microorganisms meet the safety standards, but are difficult to form a homogeneous liquid with low water solubility. Often difficult to use. Furthermore, emulsifiers and acids are often used in conventional food preservative compositions, but in general emulsifiers have a strong bitter taste, and acids have a sour taste. If these remain in equipment after washing and sterilization, the flavor of food may be affected. On the other hand, if the amount of the composition used is limited in consideration of the flavor of the food, sufficient sterilization or bacteriostatic effect may not be obtained. Therefore, sterilization of food, or the equipment in which it is handled, or the environment in which it exists, which has a homogeneous liquid and has a high bactericidal or bacteriostatic effect but has little bitterness or sourness and does not affect the flavor of food. There is a need for compositions useful for bacteriostasis.
　さらに、食品を取り扱う機器においては、殺菌処理直後だけでなく、機器の使用中にわたって減菌された状態を維持することも望まれる。したがって、素材についた微生物を殺菌又は静菌した後、そのまま継続的に該素材における微生物の増殖を抑えることを可能にする組成物の開発は、非常に有用であると考えられる。 Furthermore, it is also desirable for devices that handle food to maintain a sterilized state not only immediately after sterilization but also during the use of the device. Therefore, it is considered that the development of a composition capable of continuously suppressing the growth of microorganisms in the material after sterilizing or bacteriostatizing the microorganism attached to the material is very useful.
　本発明者らは、所定量のカプリル酸モノグリセリド及び／又はカプリン酸モノグリセリドを含む組成物にウルトラリン酸塩を添加して、そのｐＨを３．５以下に調整することにより、高い殺菌又は静菌作用を有しながらも、苦味の少ない液状組成物が得られることを見出した。さらに本発明者らは、当該液状組成物を素材に噴霧又は塗布することで、該素材における微生物の増殖を長時間にわたって抑制できることを見出した。 The inventors of the present invention have achieved high sterilization or bacteriostasis by adding ultraphosphate to a composition containing a predetermined amount of caprylic acid monoglyceride and / or capric acid monoglyceride and adjusting its pH to 3.5 or less. It has been found that a liquid composition with little bitterness can be obtained while having an action. Furthermore, the present inventors have found that the growth of microorganisms in the material can be suppressed for a long time by spraying or applying the liquid composition to the material.
　したがって、本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、殺菌又は静菌用液状組成物を提供する。
　また本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、対象への抗菌性付与用液状組成物を提供する。 Therefore, the present invention contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Provided is a liquid composition for sterilization or bacteriostasis that has a pH of 3.5 or less.
The present invention also includes at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. And a liquid composition for imparting antibacterial properties to a subject having a pH of 3.5 or lower.
　さらに本発明は、液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、殺菌又は静菌方法を提供する。
　さらに本発明は、液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、対象への抗菌性付与方法を提供する。 Further, the present invention includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition, and the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride Provided is a bactericidal or bacteriostatic method comprising a seed and an ultraphosphate, wherein the total concentration of caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more and pH 3.5 or less.
Further, the present invention includes spraying or applying the liquid composition to the object, or immersing the object in the liquid composition, and the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride Provided with a method for imparting antibacterial properties to a subject, comprising seeds and ultraphosphate, wherein the total concentration of caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more and pH 3.5 or less To do.
　さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌のための使用を提供する。
　さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与のための使用を提供する。 Furthermore, the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more. The use of a liquid composition having a pH of 3.5 or lower for sterilization or bacteriostasis is provided.
Furthermore, the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more. The use of a liquid composition having a pH of 3.5 or less for imparting antibacterial properties to a subject is provided.
　さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌用液状組成物の製造のための使用を提供する。
　さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与用液状組成物の製造のための使用を提供する。 Furthermore, the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more. The use of a liquid composition having a pH of 3.5 or less for the production of a liquid composition for bactericidal or bacteriostatic treatment is provided.
Furthermore, the present invention contains at least one selected from caprylic monoglyceride and capric monoglyceride and an ultraphosphate, and the total concentration of the caprylic monoglyceride and capric monoglyceride is 0.05% by mass or more. The use of a liquid composition having a pH of 3.5 or less for the production of a liquid composition for imparting antibacterial properties to a subject is provided.
　本発明の殺菌又は静菌用液状組成物は、液状であるため使用しやすく、また高い殺菌又は静菌効果を有しながらも苦味が少なく食品の風味に影響を与えにくいので、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有効である。また、本発明の液状組成物は、素材に適用すれば、該素材についた微生物を殺菌又は静菌することができ、さらにその後長時間にわたって、該素材における微生物の増殖を継続的に抑えることができる。したがって、本発明は、恒常的に微生物増殖抑制が望まれる分野、例えば、食品の製造、加工、保存等の分野において有効である。 Since the liquid composition for bactericidal or bacteriostatic use of the present invention is liquid, it is easy to use and has a high bactericidal or bacteriostatic effect but has little bitterness and hardly affects the flavor of food. It is effective for sterilization or bacteriostasis of the equipment that handles or the environment where it exists. Moreover, when the liquid composition of the present invention is applied to a material, it can sterilize or bacterioculate microorganisms attached to the material, and can continuously suppress the growth of microorganisms in the material for a long time thereafter. it can. Therefore, the present invention is effective in the field where microbial growth suppression is constantly desired, for example, the field of food production, processing, storage and the like.
　本発明は、殺菌又は静菌用組成物を提供する。本発明の殺菌又は静菌用組成物は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有する。 The present invention provides a bactericidal or bacteriostatic composition. The bactericidal or bacteriostatic composition of the present invention contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride, and an ultraphosphate.
　カプリル酸モノグリセリド及びカプリン酸モノグリセリドはいずれも、従来、乳化剤として食品等に適用されている物質であり、市販されている。例えば、カプリル酸モノグリセリドは、サンソフト（登録商標）７００ｐ－２（太陽化学株式会社）、ポエムＭ－１００（理研ビタミン株式会社）など、カプリン酸モノグリセリドは、サンソフト（登録商標）７６０（太陽化学株式会社）、ポエムＭ－２００（理研ビタミン株式会社）などの商品名で販売されている。 Both caprylic acid monoglyceride and capric acid monoglyceride have been conventionally applied to foods as emulsifiers and are commercially available. For example, caprylic acid monoglyceride is Sunsoft (registered trademark) 700p-2 (Taiyo Chemical Co., Ltd.), Poem M-100 (Riken Vitamin Co., Ltd.), and capric acid monoglyceride is Sunsoft (registered trademark) 760 (Taiyo Chemical). Co., Ltd.) and Poem M-200 (RIKEN Vitamin Co., Ltd.).
　本発明において、カプリル酸モノグリセリドとカプリン酸モノグリセリドは、いずれか一方を用いてもよいが、組み合わせて使用してもよい。本発明の組成物におけるカプリル酸モノグリセリドとカプリン酸モノグリセリドの合計濃度は、該組成物全量中、好ましくは０．０５質量％以上、さらに好ましくは０．１質量％以上が望ましい。この濃度が低いと、本発明の組成物は充分な殺菌又は静菌特性を得られない。一方、溶解性、経済性の観点から、本発明の組成物におけるカプリル酸モノグリセリドとカプリン酸モノグリセリドの合計濃度は、該組成物全量中、好ましくは１０．０質量％以下、より好ましくは２．０質量％以下、さらに好ましくは１．０質量％以下、なお好ましくは０．５％質量以下である。よって、本発明の組成物におけるカプリル酸モノグリセリドとカプリン酸モノグリセリドの合計濃度の範囲の例としては、該組成物全量中、好ましくは０．０５～１０．０質量％、より好ましくは０．０５～２．０質量％、さらに好ましくは０．０５～１．０質量％、さらに好ましくは０．０５～０．５質量％、さらに好ましくは０．１～０．５質量％が挙げられる。 In the present invention, either caprylic acid monoglyceride or capric acid monoglyceride may be used, or may be used in combination. The total concentration of caprylic acid monoglyceride and capric acid monoglyceride in the composition of the present invention is preferably 0.05% by mass or more, more preferably 0.1% by mass or more in the total amount of the composition. When this concentration is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, from the viewpoint of solubility and economy, the total concentration of caprylic acid monoglyceride and capric acid monoglyceride in the composition of the present invention is preferably 10.0% by mass or less, more preferably 2.0% or less in the total amount of the composition. It is at most mass%, more preferably at most 1.0 mass%, still more preferably at most 0.5% mass. Therefore, an example of the range of the total concentration of caprylic acid monoglyceride and capric acid monoglyceride in the composition of the present invention is preferably 0.05 to 10.0% by mass, more preferably 0.05 to 10.0% by mass in the total amount of the composition. 2.0% by mass, more preferably 0.05 to 1.0% by mass, still more preferably 0.05 to 0.5% by mass, and still more preferably 0.1 to 0.5% by mass.
　ウルトラリン酸は、酸性ヘキサメタリン酸とも称される。本発明で使用されるウルトラリン酸塩としては、ウルトラリン酸ナトリウムが好ましい。ウルトラリン酸ナトリウムは、従来、結着剤、ｐＨ調整剤等として食品等に適用されている。ウルトラリン酸塩は、市販されており、例えばウルトラポリホス（株式会社ポリホス化学研究所）、ウルトラポリン（太平化学産業株式会社）などの商品名で販売されている。 Ultraphosphoric acid is also called acidic hexametaphosphoric acid. As the ultraphosphate used in the present invention, sodium ultraphosphate is preferred. Conventionally, sodium ultraphosphate has been applied to foods and the like as a binder, a pH adjuster and the like. Ultraphosphate is commercially available, for example, sold under trade names such as Ultrapolyphos (Polyphos Chemical Laboratory Co., Ltd.) and Ultraporin (Taihei Chemical Sangyo Co., Ltd.).
　本発明の組成物におけるウルトラリン酸塩の濃度は、カプリル酸モノグリセリド又はカプリン酸モノグリセリドの濃度によっても異なり得るが、該組成物全量中、好ましくは０．０１５質量％以上、より好ましくは０．０１８質量％以上、さらに好ましくは０．０２質量％以上である。ウルトラリン酸塩の濃度が低いと、本発明の組成物が充分な殺菌又は静菌特性を得られない。他方、本発明の組成物におけるウルトラリン酸塩の濃度は、該組成物全量中、好ましくは３．８質量％以下、より好ましくは２．５質量％以下、さらに好ましくは１．０質量％以下である。ウルトラリン酸塩の濃度が高すぎると、本発明の組成物のｐＨが低くなり、金属の腐食など、本発明の組成物の適用環境に悪影響を及ぼすおそれがある。よって、本発明の組成物におけるウルトラリン酸塩の濃度の範囲の例としては、該組成物全量中、好ましくは０．０１５～３．８質量％、より好ましくは０．０１８～２．５質量％、さらに好ましくは０．０２～１．０質量％が挙げられる。 The concentration of ultraphosphate in the composition of the present invention may vary depending on the concentration of caprylic acid monoglyceride or capric acid monoglyceride, but is preferably 0.015% by mass or more, more preferably 0.018% in the total amount of the composition. It is at least mass%, more preferably at least 0.02 mass%. When the concentration of ultraphosphate is low, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, the concentration of the ultraphosphate in the composition of the present invention is preferably 3.8% by mass or less, more preferably 2.5% by mass or less, and further preferably 1.0% by mass or less in the total amount of the composition. It is. If the concentration of the ultraphosphate is too high, the pH of the composition of the present invention is lowered, which may adversely affect the application environment of the composition of the present invention, such as metal corrosion. Therefore, as an example of the range of the concentration of ultraphosphate in the composition of the present invention, the total amount of the composition is preferably 0.015 to 3.8% by mass, more preferably 0.018 to 2.5% by mass. %, More preferably 0.02 to 1.0% by mass.
　本発明の殺菌又は静菌用組成物に含まれ得るカプリル酸モノグリセリド、カプリン酸モノグリセリド、及びウルトラリン酸塩は、いずれも食品添加物として許容されている物質である。したがって、本発明の組成物は、一般的な洗浄剤や殺菌剤と比べて人体に対する安全性が高い。また、本発明の組成物は、従来の食品保存料や殺菌成分のような強い苦味がないため、食品とともに摂取しても、該食品の風味に影響しにくい。したがって、本発明の組成物は、食品関連分野への適用、例えば、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有用である。 Caprylic acid monoglyceride, capric acid monoglyceride, and ultraphosphate that can be included in the composition for bactericidal or bacteriostatic treatment of the present invention are all substances that are acceptable as food additives. Therefore, the composition of the present invention has higher safety for the human body than general cleaning agents and disinfectants. Moreover, since the composition of the present invention does not have a strong bitter taste like conventional food preservatives and bactericidal components, even if it is taken together with food, it hardly affects the flavor of the food. Therefore, the composition of the present invention is useful for application in food-related fields, for example, sterilization or bacteriostasis of food, or the equipment in which it is handled or the environment in which it exists.
　本発明の組成物は、カプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩以外の他の成分、例えば、ウルトラリン酸以外のリン酸（ポリリン酸、ピロリン酸、メタリン酸）、フィチン酸、クエン酸、グルコン酸、フマル酸、マレイン酸、マロン酸、酒石酸、コハク酸、リンゴ酸、アジピン酸、塩酸などの、ウルトラリン酸以外の有機酸及び無機酸、ならびにそれらの塩からなる群より選択される少なくとも１種、食品に使用可能なｐＨ調整剤、カプリル酸モノグリセリド及びカプリン酸モノグリセリド以外の脂肪酸エステルなどを含有していてもよい。ただし、これらの他の成分の種類及び含有量は、本発明の組成物に必要な殺菌又は静菌特性が失われない程度に制限される。成分の溶解性又は組成物の品質の安定性の観点からは、本発明の組成物は、Ｃ１１以上の鎖長の脂肪酸エステルを含有しないことが好ましい。カプリル酸モノグリセリド及びカプリン酸モノグリセリド以外の脂肪酸エステルの含有量としては５質量％以下が好ましい。より好ましくは、本発明の組成物における当該他の成分の含有量は、５質量％以下である。 The composition of the present invention comprises caprylic acid monoglyceride, capric acid monoglyceride, other components other than ultraphosphate, for example, phosphoric acid other than ultraphosphoric acid (polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid), phytic acid, citric acid , Gluconic acid, fumaric acid, maleic acid, malonic acid, tartaric acid, succinic acid, malic acid, adipic acid, hydrochloric acid, and other organic and inorganic acids other than ultraphosphoric acid, and their salts You may contain the fatty acid ester other than the pH adjuster which can be used for a foodstuff, caprylic acid monoglyceride, capric acid monoglyceride, etc. at least. However, the types and contents of these other components are limited to such an extent that the bactericidal or bacteriostatic properties necessary for the composition of the present invention are not lost. From the viewpoint of the solubility of the components or the stability of the quality of the composition, the composition of the present invention preferably does not contain a fatty acid ester having a chain length of C11 or more. The content of fatty acid esters other than caprylic monoglyceride and capric monoglyceride is preferably 5% by mass or less. More preferably, content of the said other component in the composition of this invention is 5 mass% or less.
　本発明の組成物は、さらにアルコール類を含有していてもよい。本発明の組成物に使用されるアルコール類としては、例えばエタノールなどの食品に使用可能なアルコール類が挙げられる。アルコール類の添加により、カプリル酸モノグリセリド又はカプリン酸モノグリセリドの溶解性が向上し、本発明の組成物の品質が安定する。本発明の組成物におけるアルコール類の含有量は、上述したカプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部以下であればよく、好ましくは該カプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部であり、より好ましくは、該組成物全量中２０～６０質量％である。 The composition of the present invention may further contain alcohols. Examples of alcohols used in the composition of the present invention include alcohols that can be used in foods such as ethanol. By adding alcohols, the solubility of caprylic acid monoglyceride or capric acid monoglyceride is improved, and the quality of the composition of the present invention is stabilized. The content of alcohols in the composition of the present invention may be equal to or less than the remainder of the caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components described above, preferably the caprylic acid monoglyceride, capric acid monoglyceride, The balance of ultraphosphate and other components, more preferably 20 to 60% by mass in the total amount of the composition.
　本発明の組成物は、さらに水性溶媒を含有していてもよい。好ましい水性溶媒としては、例えば水が挙げられる。本発明の組成物における該水性溶媒の含有量は、上述したカプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部以下であればよく、好ましくは該カプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩、他の成分及びアルコール類の残部であり、さらに好ましくは２０質量％以上である。 The composition of the present invention may further contain an aqueous solvent. A preferable aqueous solvent includes, for example, water. The content of the aqueous solvent in the composition of the present invention may be equal to or less than the remainder of the caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components described above, and preferably the caprylic acid monoglyceride and capric acid monoglyceride. , Ultraphosphate, other components and the balance of alcohols, more preferably 20% by mass or more.
　本発明の組成物の一例は、上記水性溶媒に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解した液状組成物である。本発明の組成物の別の一例は、上記アルコール類に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解した液状組成物である。本発明の組成物の別の一例は、上記アルコール類と水性溶媒との混合溶媒に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解した液状組成物である。本発明の組成物の好ましい一例は、エタノール／水の混合溶媒に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解したエタノール水溶液である。該エタノール／水の混合溶媒としては、２０～６０質量％エタノール水溶液が望ましい。該アルコール類と水性溶媒との混合溶媒、又は該エタノール／水の混合溶媒の本発明の組成物における含有量は、上述したカプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部であればよい。 An example of the composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in the aqueous solvent. Another example of the composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in the above alcohols. Another example of the composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in a mixed solvent of the above alcohols and an aqueous solvent. It is. A preferred example of the composition of the present invention is an aqueous ethanol solution in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in a mixed solvent of ethanol / water. The mixed solvent of ethanol / water is preferably a 20 to 60% by mass aqueous ethanol solution. The content of the mixed solvent of the alcohols and the aqueous solvent or the mixed solvent of the ethanol / water in the composition of the present invention is the above-described caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate, and the remainder of the other components. If it is.
　本発明の組成物のｐＨは、好ましくは３．５以下であり、より好ましくは３．０以下である。ｐＨが高くなると、本発明の組成物が充分な殺菌又は静菌特性を得られない。他方、本発明の組成物のｐＨは１．５以上が好ましい。ｐＨが低くなると、金属の腐食など、本発明の組成物の適用環境に悪影響を及ぼすおそれがある。よって、本発明の組成物のｐＨの範囲の例としては、好ましくは１．５～３．５、より好ましくは１．５～３．０が挙げられる。 The pH of the composition of the present invention is preferably 3.5 or less, more preferably 3.0 or less. When the pH is high, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, the pH of the composition of the present invention is preferably 1.5 or more. When pH becomes low, there exists a possibility of having a bad influence on the application environment of the composition of this invention, such as corrosion of a metal. Therefore, examples of the pH range of the composition of the present invention include preferably 1.5 to 3.5, more preferably 1.5 to 3.0.
　本発明の組成物のｐＨは、主にウルトラリン酸塩の添加によって調整され得る。当該ｐＨ調整には、ウルトラリン酸塩以外の他の酸や、食品に使用可能なｐＨ調整剤を用いてもよい。 The pH of the composition of the present invention can be adjusted mainly by the addition of ultraphosphate. For the pH adjustment, an acid other than ultraphosphate or a pH adjuster usable for food may be used.
　本発明の組成物は、優れた殺菌又は静菌作用を有しており、各種物質の殺菌又は静菌に使用することができる。本明細書において、殺菌とは、微生物を死滅させること（但し完全な死滅に限られない）をいい、また静菌とは、微生物の増殖を抑制することをいう。したがって、本発明の殺菌又は静菌用組成物を適用された対象においては、微生物の増殖が抑制されるか、又は微生物の数が減少する。 The composition of the present invention has an excellent bactericidal or bacteriostatic action, and can be used for bactericidal or bacteriostatic of various substances. In this specification, sterilization refers to killing microorganisms (but not limited to complete killing), and bacteriostasis refers to inhibiting the growth of microorganisms. Therefore, in the subject to which the sterilizing or bacteriostatic composition of the present invention is applied, the growth of microorganisms is suppressed or the number of microorganisms is reduced.
　本発明の組成物により殺菌又は静菌される微生物の種類としては、特に限定されないが、例えば、Ｗｅｉｓｓｅｌｌａ属、Ｌｅｕｃｏｎｏｓｔｏｃ属等の乳酸菌、Ｋｌｅｂｓｉｅｌｌａ属、Ｅｎｔｅｒｏｂａｃｔｅｒ属等の大腸菌群、Ｃａｎｄｉｄａ属、Ｃｒｙｐｔｏｃｏｃｃｕｓ属等の酵母、Ｃｌａｄｏｓｐｏｒｉｕｍ属等のカビ、大腸菌（Ｅｓｃｈｅｒｉｃｈｉａ　ｃｏｌｉ）、サルモネラ菌（Ｓａｌｍｏｎｅｌｌａ　ｅｎｔｅｒｉｃａ）、黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ　ａｕｒｅｕｓ）、緑膿菌（Ｐｓｅｕｄｏｍｏｎａｓ　ａｅｒｕｇｉｎｏｓａ）、リステリア菌（Ｌｉｓｔｅｒｉａ　ｍｏｎｏｃｙｔｏｇｅｎｅｓ）、腸炎ビブリオ（Ｖｉｂｒｉｏ　ｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ）、などが挙げられる。特に、乳酸菌等の耐酸性菌は、通常の微生物が生育を阻害される低ｐＨ領域でも生存するため従来除去しにくいものであったが、これらも本発明の組成物により殺菌又は静菌することができる。このように、本発明の組成物は、耐酸性菌等の従来除去しにくかった微生物種にも有用であるため、利用価値が高い。 The kind of microorganisms sterilized or bacteriostatic by the composition of the present invention is not particularly limited. Yeast, molds of the genus Cladosporium, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa L. , Etc. In particular, acid-resistant bacteria such as lactic acid bacteria have conventionally been difficult to remove because they survive even in the low pH range where normal microorganisms are inhibited from growing, but these are also sterilized or bacteriostatic by the composition of the present invention. Can do. Thus, the composition of the present invention has high utility value because it is also useful for microbial species that have been difficult to remove in the past, such as acid-resistant bacteria.
　本発明の組成物は、各種素材の殺菌又は静菌に使用することができる。本発明の組成物の適用対象は、本発明の組成物との接触が許容されるものである限り、特に限定されない。本発明の組成物の適用対象の好ましい例としては、食品、食品を取り扱う機器、及び食品が存在する環境が挙げられる。該食品を取り扱う機器としては、製造、加工、流通、保存、調理等の過程で食品と接触する可能性のある機器、例えば、食品の製造もしくは加工工場の製造ラインの機器、食品の保存もしくは運搬用の容器、調理器具、調理台、作業者の手袋やエプロン、作業着、などが挙げられる。食品が存在する環境としては、食品を取り扱う環境、例えば食品の製造、加工、流通、保存、調理などが行われる環境、より詳細には、食品の製造もしくは加工工場、食品保管庫、調理場等の壁、床、天井、柱、ドア、窓、換気孔などが挙げられる。 The composition of the present invention can be used for sterilization or bacteriostatic of various materials. The application target of the composition of the present invention is not particularly limited as long as contact with the composition of the present invention is allowed. Preferable examples of the application target of the composition of the present invention include foods, equipment for handling foods, and environments where foods are present. Equipment that handles the food includes equipment that may come into contact with food in the process of production, processing, distribution, storage, cooking, etc., for example, equipment on the production line of a food production or processing plant, storage or transportation of food Containers, cooking utensils, cooking tables, workers' gloves and apron, work clothes, etc. The environment in which food exists is the environment in which food is handled, for example, the environment in which food is manufactured, processed, distributed, stored, cooked, etc., and more specifically, the food manufacturing or processing factory, food storage, cooking place, etc. Walls, floors, ceilings, pillars, doors, windows, ventilation holes.
　本発明の殺菌又は静菌用組成物による対象の殺菌又は静菌は、該本発明の組成物を該対象に接触させることによって行われ得る。本発明の殺菌又は静菌用組成物は、液状であるため、殺菌又は静菌すべき対象への適用が容易である。例えば、本発明の液状組成物は、殺菌又は静菌対象の物質（例えば食品など）中に混合されてもよい。また例えば、本発明の液状組成物を、殺菌又は静菌対象の物質に噴霧もしくは塗布するか、又は本発明の液状組成物に該対象の物質を浸漬させてもよい。好ましくは、本発明の液状組成物は、殺菌又は静菌すべき対象に対して噴霧又は塗布される。本発明の液状組成物と接触した対象は、殺菌又は静菌される。 The sterilization or bacteriostasis of an object by the sterilization or bacteriostatic composition of the present invention can be performed by bringing the composition of the present invention into contact with the object. Since the composition for bactericidal or bacteriostatic of the present invention is liquid, it can be easily applied to a subject to be bactericidal or bacteriostatic. For example, the liquid composition of the present invention may be mixed in a substance to be sterilized or bacteriostatic (for example, food). Further, for example, the liquid composition of the present invention may be sprayed or applied to a substance to be sterilized or bacteriostatic, or the target substance may be immersed in the liquid composition of the present invention. Preferably, the liquid composition of the present invention is sprayed or applied to a subject to be sterilized or bacteriostatic. Objects that come into contact with the liquid composition of the present invention are sterilized or bacteriostatic.
　本発明の好ましい態様においては、殺菌又は静菌すべき対象に対して本発明の組成物を噴霧もしくは塗布するか、又は浸漬させることで、該本発明の組成物と該対象とを接触させる。さらに、該本発明の組成物と接触させた対象を乾燥させてもよい。乾燥の方法としては、静置による乾燥、風乾、乾燥機による乾燥などが挙げられるが、特に限定されない。該乾燥により、本発明の組成物に含まれる揮発性成分、例えば水性溶媒やエタノールは、揮発して該対象から除去される。該揮発性成分が除去された後でも、本発明の組成物を適用した対象においては、殺菌又は静菌作用が持続する。したがって、本態様において、本発明の組成物は、液体の状態で対象に対して殺菌又は静菌力を発揮するだけでなく、乾燥物状態でも、該対象における微生物の増殖を抑える。 In a preferred embodiment of the present invention, the composition of the present invention is brought into contact with the subject by spraying, applying, or immersing the composition of the present invention on the subject to be sterilized or bacteriostatic. Furthermore, the object brought into contact with the composition of the present invention may be dried. Examples of the drying method include, but are not particularly limited to, drying by standing, air drying, and drying by a dryer. By the drying, volatile components contained in the composition of the present invention, such as an aqueous solvent and ethanol, are volatilized and removed from the target. Even after the removal of the volatile components, the bactericidal or bacteriostatic action continues in the subject to which the composition of the present invention is applied. Therefore, in this embodiment, the composition of the present invention not only exhibits sterilization or bacteriostatic activity against a subject in a liquid state, but also suppresses the growth of microorganisms in the subject even in a dry matter state.
　したがって、本発明の組成物の別の態様は、対象への抗菌性付与用液状組成物であり得る。本発明の組成物を、抗菌性を付与したい対象に噴霧、塗布、浸漬等により付着させることにより、該対象に抗菌性が付与される。必要に応じて又は好ましくは、さらに該本発明の組成物を付着させた対象を乾燥させる。本明細書において、対象の「抗菌性」とは、該対象の表面に付着又は内部に侵入した微生物を殺菌又は静菌することができるという、該対象の性質をいう。本態様は、恒常的に微生物増殖を防止したい環境、又はそこで使用される素材や機器、例えば、食品を取り扱う環境又はそこで使用される食品取り扱い用機器に対して、非常に有用である。 Therefore, another embodiment of the composition of the present invention may be a liquid composition for imparting antibacterial properties to a subject. The antibacterial property is imparted to the subject by adhering the composition of the present invention to the subject to be imparted with the antibacterial property by spraying, coating, dipping, or the like. If necessary or preferably, the object to which the composition of the present invention is further adhered is dried. In the present specification, “antibacterial” of a subject refers to the property of the subject that microorganisms attached to or invaded the surface of the subject can be sterilized or bacteriostatic. This aspect is very useful for an environment where it is desired to constantly prevent microbial growth, or for materials and equipment used therein, for example, an environment where food is handled or food handling equipment used there.
　本発明の組成物は、対象の殺菌又は静菌に用いる場合、及び対象への抗菌性付与に用いる場合のいずれにおいても、対象の面積１００ｃｍ2あたり０．１～５．０ｍＬ程度の用量で使用されることが好ましく、対象の面積１００ｃｍ2あたり０．５～１．０ｍＬ程度の用量で使用されることがより好ましい。好ましくは、この用量の組成物を、対象に対して、好ましくは噴霧又は塗布により、均等に付着させる。 The composition of the present invention is used at a dose of about 0.1 to 5.0 mL per 100 cm 2 area of the subject, whether used for sterilization or bacteriostasis of the subject, or for imparting antibacterial properties to the subject. Preferably, it is used at a dose of about 0.5 to 1.0 mL per 100 cm 2 area of the subject. Preferably, this dose of the composition is evenly applied to the subject, preferably by spraying or application.
　以下、実施例を挙げて、本発明をさらに詳細に説明するが、本発明はこれらの実施例にのみ限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited only to these examples.
参考例
　室温下で、カプリル酸モノグリセリド又はカプリン酸モノグリセリドと精製水を混合して、表１に示す組成の液状組成物を調製した。得られた組成物の呈味性（苦味）を評価した。評価は、訓練を受けた１０名のパネルにより下記評価基準にて評価し、平均点を求めた。
（呈味性の評価基準）
　　５点：苦味がごくわずかで気にならない
　　４点：少しの苦味があるが許容できる
　　３点：苦味が感じられる
　　２点：苦味を感じ、口に入れると支障がある
　　１点：明らかな苦味があり、食べることに抵抗がある Reference Example Capillic acid monoglyceride or capric acid monoglyceride and purified water were mixed at room temperature to prepare a liquid composition having the composition shown in Table 1. The taste (bitterness) of the obtained composition was evaluated. The evaluation was performed by 10 trained panels according to the following evaluation criteria, and the average score was obtained.
(Evaluation criteria for taste)
5 points: Bitterness is negligible and not worrisome 4 points: Slight bitterness but acceptable 3 points: Bitterness is felt 2 points: Bitterness is felt, and there is a problem when put in the mouth 1 point: Clear bitterness There is resistance to eating
　結果を表１に示す。カプリル酸モノグリセリド又はカプリン酸モノグリセリドの濃度が上がるほど、組成物はより強い苦味を呈し、濃度０．１質量％以上では食用に許容できないほどの苦みを有していた。 The results are shown in Table 1. The higher the concentration of caprylic acid monoglyceride or capric acid monoglyceride, the stronger the bitter taste, and the bitterness unacceptably edible at a concentration of 0.1% by mass or more.
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
試験例１
　室温下で、カプリル酸モノグリセリド又はカプリン酸モノグリセリドと精製水を混合し、これに各種の酸を加えてｐＨを調整して、表２に示す組成の液状組成物を調製した。得られた組成物の呈味性（苦味）を、参考例と同様の基準で評価した。 Test example 1
Capillic acid monoglyceride or capric acid monoglyceride and purified water were mixed at room temperature, and various acids were added thereto to adjust the pH, thereby preparing liquid compositions having the compositions shown in Table 2. The taste (bitterness) of the obtained composition was evaluated based on the same criteria as in the reference example.
　結果を表２～３に示す。ウルトラリン酸塩以外の酸を含む組成物は、苦味が強かった。また、ウルトラリン酸塩以外のリン酸塩を使用した場合、多量に使用しても組成物のｐＨが充分に下がらなかった。 The results are shown in Tables 2-3. The composition containing an acid other than ultraphosphate had a strong bitterness. Further, when a phosphate other than ultraphosphate was used, the pH of the composition was not sufficiently lowered even when used in a large amount.
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000003
試験例２
　室温下で、カプリル酸モノグリセリド又はカプリン酸モノグリセリド、エタノール及び滅菌済み精製水を１：２：２の割合で混合して溶液を調製し、これにウルトラリン酸塩又は他のリン酸塩、及び残余のエタノールと滅菌済み精製水を添加して表４～５記載の液状組成物（溶液）を得た。得られた各液状組成物について、下記の手順で殺菌又は静菌特性を評価した。 Test example 2
At room temperature, a solution is prepared by mixing caprylic acid monoglyceride or capric acid monoglyceride, ethanol and sterilized purified water in a ratio of 1: 2: 2, to which ultraphosphate or other phosphate, and the remainder Ethanol and sterilized purified water were added to obtain liquid compositions (solutions) shown in Tables 4-5. About each obtained liquid composition, the bactericidal or bacteriostatic characteristic was evaluated in the following procedure.
〔殺菌又は静菌特性評価〕
（１）サンプル菌液の調製
　対象菌には、４種の乳酸菌Ｗｅｉｓｓｅｌｌａ　ｖｉｒｉｄｅｓｃｅｎｓ、Ｌｅｕｃｏｎｏｓｔｏｃ　ｍｅｓｅｎｔｅｒｏｉｄｅｓ、Ｌｅｕｃｏｎｏｓｔｏｃ　ｃｉｔｒｅｕｍ、及びＬｅｕｃｏｎｏｓｔｏｃ　ｐｓｅｕｄｏｍｅｓｅｎｔｅｒｏｉｄｅｓを用いた。対象菌のシングルコロニーを白金耳を用いて数コロニー釣菌し、ＴＳＢ培地に植菌して３０℃で２０時間種培養したものを乳酸菌液（１０7ｃｆｕ／ｍＬ）とした。得られた乳酸菌液を、滅菌済み精製水１０ｍＬに０．１ｍＬ混合し、さらにこれを１０倍希釈した。４種の乳酸菌の１０倍希釈液を等量混合してサンプル菌液を調製した。 [Bactericidal or bacteriostatic characteristics evaluation]
(1) Preparation of Sample Bacterial Solution Four types of lactic acid bacteria Weissella viridescens, Leuconostoc mesenteroides, Leuconostoc citreum, and Leuconostoc pseudomesenteroides were used. A single colony of the target bacterium was picked using a platinum loop, inoculated into a TSB medium, and seeded and cultured at 30 ° C. for 20 hours to obtain a lactic acid bacteria solution (10 7 cfu / mL). The obtained lactic acid bacteria solution was mixed with 0.1 mL of sterilized purified water and further diluted 10 times. A sample bacterial solution was prepared by mixing equal amounts of a 10-fold diluted solution of four types of lactic acid bacteria.
（２）液状組成物の適用及び菌数測定
　塗布直後及び１８時間後の液状組成物による静菌特性を調べた。滅菌プラスチックシャーレを二群に分けた。一方の群では、各液状組成物を１．０ｍＬずつ空のシャーレに入れ、コンラージ棒で均一に塗布し、次いでこれにサンプル菌液を１．０ｍＬ接種し、２０秒間コンラージ棒で混合しながら塗り広げた（０ｈ群）。もう一方の群では、各液状組成物を１．０ｍＬずつ空のシャーレに入れ、コンラージ棒で均一に塗布した後、安全キャビネット内で１８時間風乾し、水分とエタノールを完全に蒸発させた。これにサンプル菌液を１．０ｍＬ接種し、２０秒間コンラージ棒で混合しながら塗り広げた（１８ｈ群）。初発菌数は１０5～１０6ｃｆｕ/ｍＬに調整した。サンプル菌液を塗布したシャーレを、５分間静置後、シャーレ内の液体を採取し、ＭＲＳ寒天培地を用いて菌数を測定した。 (2) Application of liquid composition and measurement of bacterial count The bacteriostatic characteristics of the liquid composition immediately after application and 18 hours later were examined. Sterile plastic dishes were divided into two groups. In one group, 1.0 mL of each liquid composition is placed in an empty petri dish, and uniformly applied with a large stick, then 1.0 mL of the sample bacterial solution is inoculated, and mixed for 20 seconds with mixing with a large stick. Spread (0h group). In the other group, 1.0 mL of each liquid composition was placed in an empty petri dish, and uniformly applied with a large stick, then air-dried in a safety cabinet for 18 hours to completely evaporate water and ethanol. This was inoculated with 1.0 mL of the sample bacterial solution and spread with mixing with a large rod for 20 seconds (18h group). The initial bacterial count was adjusted to 10 5 to 10 6 cfu / mL. The petri dish to which the sample bacterial solution was applied was allowed to stand for 5 minutes, and then the liquid in the petri dish was collected and the number of bacteria was measured using an MRS agar medium.
（３）殺菌又は静菌特性評価
　（２）で測定した菌数をもとに、下記評価基準にて各組成物の殺菌又は静菌特性を評価した。
　Ａ：初発の１／１０００以下に菌数が減少
　Ｂ：初発の１／１００以下～１／１０００超に菌数が減少
　Ｃ：初発の１／１０以下～１／１００超に菌数が減少
　Ｄ：菌数が初発の１／１０よりも多い (3) Bactericidal or bacteriostatic characteristics evaluation Based on the number of bacteria measured in (2), the bactericidal or bacteriostatic characteristics of each composition were evaluated according to the following evaluation criteria.
A: The number of bacteria decreased to 1/1000 or less of the first time. B: The number of bacteria decreased to 1/100 or less of the first time to more than 1/1000 C. The number of bacteria decreased to 1/10 or less of the first time and more than 1/100 of the first time. D : The number of bacteria is more than 1/10 of the first
　結果を表４～５に示す。ウルトラリン酸塩以外のリン酸塩を含む組成物は、殺菌又は静菌作用を持続できなかった。 The results are shown in Tables 4-5. Compositions containing phosphates other than ultraphosphates could not sustain bactericidal or bacteriostatic effects.
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000005
試験例３
　試験例２と同様の手順で、ただしカプリル酸モノグリセリド又はカプリン酸モノグリセリドの濃度を変更して、表６～７記載の液状組成物を調製した。得られた組成物について、参考例と同様の手順で呈味性（苦味）を評価し、また試験例２と同様の手順で殺菌又は静菌特性を評価した。結果を表６～７に示す。カプリル酸モノグリセリド又はカプリン酸モノグリセリドを０．０５質量％以上含有する組成物は、苦味がなく、かつ持続的な殺菌又は静菌作用を有していた。 Test example 3
In the same procedure as in Test Example 2, except that the concentration of caprylic acid monoglyceride or capric acid monoglyceride was changed, liquid compositions shown in Tables 6 to 7 were prepared. About the obtained composition, taste (bitter taste) was evaluated in the same procedure as the reference example, and bactericidal or bacteriostatic properties were evaluated in the same procedure as in Test Example 2. The results are shown in Tables 6-7. The composition containing 0.05% by mass or more of caprylic acid monoglyceride or capric acid monoglyceride had no bitter taste and had a continuous bactericidal or bacteriostatic effect.
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000007
試験例４
　試験例２と同様の手順で、ただしウルトラリン酸塩の濃度を変更して、表８～９記載の液状組成物を調製し、試験例３と同様の手順で、呈味性、及び殺菌又は静菌特性を評価した。結果を表８～９に示す。ウルトラリン酸塩の濃度が低い組成物は苦味を有し、また持続的な殺菌又は静菌作用を示さなかった。 Test example 4
A liquid composition shown in Tables 8 to 9 was prepared in the same procedure as in Test Example 2 except that the concentration of ultraphosphate was changed. Bacteriostatic properties were evaluated. The results are shown in Tables 8-9. The composition with a low concentration of ultraphosphate had a bitter taste and did not show a continuous bactericidal or bacteriostatic effect.
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000009
試験例５
　室温下で、Ｃ８～Ｃ１２脂肪酸モノグリセリドと精製水を混合し、これにウルトラリン酸Ｎａを加えてｐＨを調整し、表１０に示す組成の液状組成物を調製した。得られた組成物の呈味性（苦味）及び液の均質さ（成分の溶解性）を評価した。呈味性については、参考例と同様の基準で評価した。液の均質さについては、調製直後又は冷蔵庫で一晩放置後の組成物について、成分の析出（白濁又は沈殿）の有無を目視で確認した。 Test Example 5
C8-C12 fatty acid monoglyceride and purified water were mixed at room temperature, and the pH was adjusted by adding sodium ultraphosphate to prepare a liquid composition having the composition shown in Table 10. The taste (bitterness) and liquid homogeneity (solubility of the components) of the obtained composition were evaluated. About taste property, it evaluated on the basis similar to a reference example. About the homogeneity of the liquid, the presence or absence of precipitation of components (white turbidity or precipitation) was visually confirmed for the composition immediately after preparation or after standing overnight in a refrigerator.
　結果を表１０に示す。カプリル酸（Ｃ８）モノグリセリド及びカプリン酸（Ｃ１０）モノグリセリドを含む組成物は、成分が水に容易に溶解して均質な溶液となったが、ラウリン酸（Ｃ１２）モノグリセリドを含む組成物は、成分が水に難溶であり、均質な溶液にはならなかった。 The results are shown in Table 10. The composition containing caprylic acid (C8) monoglyceride and capric acid (C10) monoglyceride easily dissolved in water to form a homogeneous solution. However, the composition containing lauric acid (C12) monoglyceride contains It was poorly soluble in water and did not become a homogeneous solution.
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000010
試験例６
　様々な素材上における液状組成物の殺菌又は静菌作用を調べた。
　試験例２と同様の手順で、液状組成物を調製した（カプリル酸モノグリセリド ０．１５％、ウルトラリン酸Ｎａ ０．４０％、エタノール ４０．００％、水 残余；単位は質量％；ｐＨ２．４）。サンプル菌液は、試験例２と同様の手順で４種の菌を用いて調製した。
　対象素材として、金属（ステンレス板）、ゴム（ゴム手袋）、プラスチック（番重ふた）を準備した。ステンレス板及び番重ふたは、１０ｃｍ四方に切断して使用した。ゴム手袋は、１０ｃｍ四方のプラスチック板に皺のないように伸ばして両面テープで貼り、余った部分はカットして使用した。 Test Example 6
The sterilization or bacteriostatic action of the liquid composition on various materials was investigated.
A liquid composition was prepared in the same procedure as in Test Example 2 (caprylic acid monoglyceride 0.15%, ultraphosphoric acid Na 0.40%, ethanol 40.00%, water residue; unit is mass%; pH 2.4) ). A sample bacterial solution was prepared using four types of bacteria in the same procedure as in Test Example 2.
As target materials, metal (stainless steel plate), rubber (rubber gloves), and plastic (guard cover) were prepared. The stainless steel plate and watch cover were cut into 10 cm squares. The rubber gloves were stretched on a 10 cm square plastic plate so as not to be wrinkled and attached with double-sided tape, and the remaining part was cut and used.
　菌数の測定は、各素材につき以下の手順で行った。各対象素材を３群に分けた。第一の群では、液状組成物０．５ｍＬを、コンラージ棒で対象素材の上に均一に塗布し、次いでサンプル菌液０．１ｍＬを接種し、４０秒間コンラージ棒で混合しながら塗り広げた（０ｈ群）。残りの２群では、液状組成物０．５ｍＬ又は１．０ｍＬを、コンラージ棒で対象素材の上に均一に塗布した後、安全キャビネット内で１８時間風乾し、水分とエタノールを完全に蒸発させた。これにサンプル菌液１．０ｍＬを接種し、４０秒間コンラージ棒で混合しながら塗り広げた（１８ｈ群）。サンプル菌液の初発菌数は１０5～１０6ｃｆｕ/ｍＬに調整した。
　サンプル菌液を塗布した対象素材を５分間静置後、無菌パウチに入れ、上から滅菌水１０ｍＬを加え、熱シールして上下に５回程度軽く振った。パウチを開封して中の液体を採取し、ＭＲＳ寒天培地で菌数を測定した。 The number of bacteria was measured according to the following procedure for each material. Each target material was divided into 3 groups. In the first group, 0.5 mL of the liquid composition was evenly applied on the target material with a large rod, then 0.1 mL of the sample bacterial solution was inoculated, and spread with mixing with a large rod for 40 seconds ( 0h group). In the remaining two groups, 0.5 mL or 1.0 mL of the liquid composition was evenly applied onto the target material with a large stick and then air-dried in a safety cabinet for 18 hours to completely evaporate moisture and ethanol. . This was inoculated with 1.0 mL of the sample bacterial solution, and spread with mixing with a large rod for 40 seconds (group 18h). The initial bacterial count of the sample bacterial solution was adjusted to 10 5 to 10 6 cfu / mL.
The target material to which the sample bacterial solution was applied was allowed to stand for 5 minutes, then placed in a sterile pouch, added with 10 mL of sterilized water from above, heat-sealed, and gently shaken up and down about 5 times. The pouch was opened, the liquid inside was collected, and the number of bacteria was measured on an MRS agar medium.
　結果を表１１に示す。本発明の組成物が、液状及び乾燥状態のいずれにおいても、各種素材の殺菌又は静菌に有効であることが分かった。 The results are shown in Table 11. It has been found that the composition of the present invention is effective for sterilization or bacteriostasis of various materials in both liquid and dry states.
Figure JPOXMLDOC01-appb-T000011
  
Figure JPOXMLDOC01-appb-T000011
  

Claims (29)

  1. 　カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、殺菌又は静菌用液状組成物。 It contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more, and has a pH of 3.5 The following liquid composition for bactericidal or bacteriostatic purposes.
  2. 　カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、対象への抗菌性付与用液状組成物。 It contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more, and has a pH of 3.5 The following liquid composition for imparting antibacterial properties to a subject.
  3. 　ｐＨ１．５～３．５である、請求項１又は２記載の組成物。 The composition according to claim 1 or 2, which has a pH of 1.5 to 3.5.
  4. 　前記ウルトラリン酸塩の濃度が０．０１８質量％以上である、請求項１～３のいずれか１項記載の組成物。 The composition according to any one of claims 1 to 3, wherein the concentration of the ultraphosphate is 0.018% by mass or more.
  5. 　さらにエタノールを含有する、請求項１～４のいずれか１項記載の組成物。 The composition according to any one of claims 1 to 4, further comprising ethanol.
  6. 　さらに水性溶媒を含有する、請求項１～５のいずれか１項記載の組成物。 The composition according to any one of claims 1 to 5, further comprising an aqueous solvent.
  7. 　対象の面積１００ｃｍ2あたり０．１～５．０ｍＬの用量で使用される、請求項１～６のいずれか１項記載の組成物。 The composition according to any one of claims 1 to 6, which is used at a dose of 0.1 to 5.0 mL per 100 cm 2 of subject area.
  8. 　液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、
    　該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、
    殺菌又は静菌方法。     Spraying or applying the liquid composition to the subject, or immersing the subject in the liquid composition;
    The liquid composition contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Yes, and pH is 3.5 or less,
    Sterilization or bacteriostatic method.
  9. 　液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、
    　該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、
    対象への抗菌性付与方法。     Spraying or applying the liquid composition to the subject, or immersing the subject in the liquid composition;
    The liquid composition contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Yes, and pH is 3.5 or less,
    A method for imparting antibacterial properties to subjects.
  10. 　前記液状組成物がｐＨ１．５～３．５である、請求項８又は９記載の方法。 The method according to claim 8 or 9, wherein the liquid composition has a pH of 1.5 to 3.5.
  11. 　前記液状組成物における前記ウルトラリン酸塩の濃度が０．０１８質量％以上である、請求項８～１０のいずれか１項記載の方法。 The method according to any one of claims 8 to 10, wherein the concentration of the ultraphosphate in the liquid composition is 0.018% by mass or more.
  12. 　前記液状組成物がさらにエタノールを含有する、請求項８～１１のいずれか１項記載の方法。 The method according to any one of claims 8 to 11, wherein the liquid composition further contains ethanol.
  13. 　前記液状組成物がさらに水性溶媒を含有する、請求項８～１２のいずれか１項記載の方法。 The method according to any one of claims 8 to 12, wherein the liquid composition further contains an aqueous solvent.
  14. 　前記液状組成物が付着した対象を乾燥させることをさらに含む、請求項８～１３のいずれか１項記載の方法。 The method according to any one of claims 8 to 13, further comprising drying an object to which the liquid composition is adhered.
  15. 　前記液状組成物が対象の面積１００ｃｍ2あたり０．１～５．０ｍＬの用量で使用される、請求項８～１４のいずれか１項記載の方法。 The method according to any one of claims 8 to 14, wherein the liquid composition is used at a dose of 0.1 to 5.0 mL per 100 cm 2 of subject area.
  16. 　カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌のための使用。 It contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more, and has a pH of 3.5 Use of the following liquid composition for bactericidal or bacteriostatic purposes.
  17. 　カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与のための使用。 It contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more, and has a pH of 3.5 Use of the following liquid composition for imparting antibacterial properties to a subject.
  18. 　前記液状組成物が、対象に付着させ乾燥させることにより使用される、請求項１６又は１７記載の使用。 The use according to claim 16 or 17, wherein the liquid composition is used by adhering to a target and drying.
  19. 　前記液状組成物が対象の面積１００ｃｍ2あたり０．１～５．０ｍＬの用量で使用される、請求項１６～１８のいずれか１項記載の使用。 Use according to any of claims 16 to 18, wherein the liquid composition is used at a dose of 0.1 to 5.0 mL per 100 cm 2 of subject area.
  20. 　カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌用液状組成物の製造のための使用。 It contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more, and has a pH of 3.5 Use of the following liquid composition for the production of a bactericidal or bacteriostatic liquid composition.
  21. 　前記殺菌又は静菌用液状組成物が、対象に付着させ乾燥させることにより使用される、請求項２０記載の使用。 21. Use according to claim 20, wherein the sterilizing or bacteriostatic liquid composition is used by adhering to a target and drying.
  22. 　前記殺菌又は静菌用液状組成物が対象の面積１００ｃｍ2あたり０．１～５．０ｍＬの用量で使用される、請求項２０又は２１記載の使用。 The use according to claim 20 or 21, wherein the liquid composition for bactericidal or bacteriostatic use is used at a dose of 0.1 to 5.0 mL per 100 cm 2 area of interest.
  23. 　カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与用液状組成物の製造のための使用。 It contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more, and has a pH of 3.5 Use of the following liquid composition for production of a liquid composition for imparting antibacterial properties to an object.
  24. 　前記抗菌性付与用液状組成物が、対象に付着させ乾燥させることにより使用される、請求項２３記載の使用。 The use according to claim 23, wherein the liquid composition for imparting antibacterial properties is used by adhering to an object and drying.
  25. 　前記抗菌性付与用液状組成物が対象の面積１００ｃｍ2あたり０．１～５．０ｍＬの用量で使用される、請求項２３又は２４記載の使用。 The use according to claim 23 or 24, wherein the liquid composition for imparting antibacterial properties is used at a dose of 0.1 to 5.0 mL per 100 cm 2 of subject area.
  26. 　前記液状組成物がｐＨ１．５～３．５である、請求項１６～２５のいずれか１項記載の使用。 The use according to any one of claims 16 to 25, wherein the liquid composition has a pH of 1.5 to 3.5.
  27. 　前記液状組成物における前記ウルトラリン酸塩の濃度が０．０１８質量％以上である、請求項１６～２６のいずれか１項記載の使用。 The use according to any one of claims 16 to 26, wherein the concentration of the ultraphosphate in the liquid composition is 0.018% by mass or more.
  28. 　前記液状組成物がさらにエタノールを含有する、請求項１６～２７のいずれか１項記載の使用。 The use according to any one of claims 16 to 27, wherein the liquid composition further contains ethanol.
  29. 　前記液状組成物がさらに水性溶媒を含有する、請求項１６～２８のいずれか１項記載の使用。
          The use according to any one of claims 16 to 28, wherein the liquid composition further comprises an aqueous solvent.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52105211A (en) * 1976-02-27 1977-09-03 Tadao Watanabe Antiseptic for foods
JPH06217749A (en) * 1993-01-28 1994-08-09 Taiyo Kagaku Co Ltd Preservation agent for food
JP2005296021A (en) * 1993-09-14 2005-10-27 Minnesota Mining & Mfg Co <3M> Germicidal composition

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59151875A (en) * 1983-02-15 1984-08-30 Q P Corp Preservative for food
JPH06153882A (en) * 1992-07-13 1994-06-03 Asama Kasei Kk Preserving agent for food
JP4141354B2 (en) 2003-09-11 2008-08-27 昭和商事株式会社 Liquid food preservative

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52105211A (en) * 1976-02-27 1977-09-03 Tadao Watanabe Antiseptic for foods
JPH06217749A (en) * 1993-01-28 1994-08-09 Taiyo Kagaku Co Ltd Preservation agent for food
JP2005296021A (en) * 1993-09-14 2005-10-27 Minnesota Mining & Mfg Co <3M> Germicidal composition

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