JP7058257B2 - Liquid composition for sterilization or bacteriostatic - Google Patents
Liquid composition for sterilization or bacteriostatic Download PDFInfo
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- JP7058257B2 JP7058257B2 JP2019506244A JP2019506244A JP7058257B2 JP 7058257 B2 JP7058257 B2 JP 7058257B2 JP 2019506244 A JP2019506244 A JP 2019506244A JP 2019506244 A JP2019506244 A JP 2019506244A JP 7058257 B2 JP7058257 B2 JP 7058257B2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
- A23L3/3517—Carboxylic acid esters
Description
本発明は、殺菌又は静菌用組成物に関する。 The present invention relates to bactericidal or bacteriostatic compositions.
従来、食品製造においては、微生物の増殖による食品の腐敗や変質を防ぐための組成物が使用されている。特許文献１には、脂肪酸モノエステル、酸またはキレート化剤、食品グレードの界面活性剤及び媒体を含有する食品用殺菌剤組成物が記載されている。特許文献２には、リゾチーム、ヘキサメタリン酸ソーダ及びカプリン酸モノグリセリドからなる食品用保存料が記載されている。特許文献３には、カプリル酸モノグリセリド及び／又はカプリン酸モノグリセリドに、グリシン、酢酸ナトリウム、リゾチーム、ならびに重合リン酸塩を併用することを特徴とする食品用保存剤が記載されている。特許文献４には、ホースラディッシュ由来の抗菌性物質と、有機酸又はその塩、アミノ酸、低級脂肪酸エステル、シュガーエステル、ビタミンＢ１エステル、重合リン酸塩、フィチン酸、エタノール等から選択される１種又は２種以上とを含有する食品用保存剤が記載されている。特許文献５には、エタノールに、ラウリン酸、デカグリセリン脂肪酸モノエステル、ショ糖脂肪酸モノエステル及び有機酸を混合溶解させた液状の食品保存剤が記載されている。 Conventionally, in food production, a composition for preventing spoilage and deterioration of food due to the growth of microorganisms has been used. Patent Document 1 describes a food disinfectant composition containing a fatty acid monoester, an acid or a chelating agent, a food grade surfactant and a medium. Patent Document 2 describes a food preservative consisting of lysozyme, sodium hexametaphosphate and monoglyceride capric acid. Patent Document 3 describes a food preservative characterized by using glycine, sodium acetate, lysoteam, and a polymerized phosphate in combination with caprylic acid monoglyceride and / or capric acid monoglyceride. Patent Document 4 describes an antibacterial substance derived from horse radish and one selected from organic acids or salts thereof, amino acids, lower fatty acid esters, sugar esters, vitamin B1 esters, polymerized phosphates, phytic acid, ethanol and the like. Alternatively, food preservatives containing two or more of them are described. Patent Document 5 describes a liquid food preservative in which lauric acid, decaglycerin fatty acid monoester, sucrose fatty acid monoester and an organic acid are mixed and dissolved in ethanol.
食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌のための組成物においては、その殺菌又は静菌効果が重要であるだけでなく、安全性や食品の風味に与える影響も無視できない。一方で、食品分野で微生物の増殖制御に従来使用されている組成物は、安全性の基準は満たすが、水溶性が低く均質な液状とすることが難しいため、機器等の洗浄や殺菌には使いづらいことが多い。さらに、従来の食品保存料組成物には、乳化剤や酸がしばしば使用されているが、一般に乳化剤は強い苦味を有しており、また酸には酸味がある。これらが洗浄や殺菌後に機器等に残留すると、食品の風味に影響するおそれがある。反面、食品の風味を考慮して組成物の使用量を制限すると、充分な殺菌又は静菌効果が得られなくなるおそれがある。したがって、均質な液状を呈し、かつ高い殺菌又は静菌効果を有しながらも苦味や酸味が少なく食品の風味に影響を与えにくい、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有用な組成物が求められている。 Not only is the bactericidal or bactericidal effect important in a composition for sterilizing or sterilizing food or the equipment in which it is handled or the environment in which it is located, but it also has an effect on safety and flavor of food. It cannot be ignored. On the other hand, compositions conventionally used for controlling the growth of microorganisms in the food field satisfy safety standards, but have low water solubility and it is difficult to make a homogeneous liquid, so that they are suitable for cleaning and sterilizing equipment. It is often difficult to use. Further, although emulsifiers and acids are often used in conventional food preservative compositions, emulsifiers generally have a strong bitter taste, and acids have an acidity. If these remain on equipment after cleaning or sterilization, they may affect the flavor of food. On the other hand, if the amount of the composition used is limited in consideration of the flavor of the food, a sufficient bactericidal or bacteriostatic effect may not be obtained. Therefore, sterilization or sterilization of food or equipment that handles it or the environment in which it is present, which exhibits a homogeneous liquid and has a high sterilizing or bacteriostatic effect but has less bitterness and acidity and does not easily affect the flavor of food. There is a demand for a composition useful for bacteriostatic.
さらに、食品を取り扱う機器においては、殺菌処理直後だけでなく、機器の使用中にわたって減菌された状態を維持することも望まれる。したがって、素材についた微生物を殺菌又は静菌した後、そのまま継続的に該素材における微生物の増殖を抑えることを可能にする組成物の開発は、非常に有用であると考えられる。 Further, in the equipment for handling food, it is desired to maintain the sterilized state not only immediately after the sterilization treatment but also during the use of the equipment. Therefore, it is considered very useful to develop a composition that can continuously suppress the growth of microorganisms in the material after sterilizing or sterilizing the microorganisms attached to the material.
本発明者らは、所定量のカプリル酸モノグリセリド及び／又はカプリン酸モノグリセリドを含む組成物にウルトラリン酸塩を添加して、そのｐＨを３．５以下に調整することにより、高い殺菌又は静菌作用を有しながらも、苦味の少ない液状組成物が得られることを見出した。さらに本発明者らは、当該液状組成物を素材に噴霧又は塗布することで、該素材における微生物の増殖を長時間にわたって抑制できることを見出した。 The present inventors add ultraphosphate to a composition containing a predetermined amount of caprylic acid monoglyceride and / or capric acid monoglyceride, and adjust the pH to 3.5 or less to achieve high sterilization or bacteriostatic. It has been found that a liquid composition having an action but less bitterness can be obtained. Furthermore, the present inventors have found that by spraying or applying the liquid composition to a material, the growth of microorganisms in the material can be suppressed for a long period of time.
したがって、本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、殺菌又は静菌用液状組成物を提供する。
また本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、対象への抗菌性付与用液状組成物を提供する。Therefore, the present invention contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Provided is a liquid composition for sterilization or bacteriostatic, which is present and has a pH of 3.5 or less.
Further, the present invention contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Provided is a liquid composition for imparting antibacterial activity to a subject, which has a pH of 3.5 or less.
さらに本発明は、液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、殺菌又は静菌方法を提供する。
さらに本発明は、液状組成物を対象に噴霧もしくは塗布するか、又は該液状組成物に対象を浸漬させることを含み、該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、対象への抗菌性付与方法を提供する。The invention further comprises spraying or applying the liquid composition to the subject or immersing the subject in the liquid composition, wherein the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride. Provided is a sterilization or bacteriostatic method containing seeds and an ultraphosphate, wherein the total concentration of the caprylic acid monoglyceride and the caprylic acid monoglyceride is 0.05% by mass or more and the pH is 3.5 or less.
The invention further comprises spraying or applying the liquid composition to the subject or immersing the subject in the liquid composition, wherein the liquid composition is at least one selected from caprylic acid monoglyceride and capric acid monoglyceride. Provided is a method for imparting antibacterial activity to a subject, which contains a seed and an ultraphosphate, and the total concentration of the caprylic acid monoglyceride and the caprylic acid monoglyceride is 0.05% by mass or more and the pH is 3.5 or less. do.
さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌のための使用を提供する。
さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与のための使用を提供する。Further, the present invention contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. And, the liquid composition having a pH of 3.5 or less is provided for use for sterilization or bacteriostatic.
Further, the present invention contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. , And the use of a liquid composition having a pH of 3.5 or less for imparting antibacterial activity to a subject is provided.
さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、殺菌又は静菌用液状組成物の製造のための使用を提供する。
さらに本発明は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である液状組成物の、対象への抗菌性付与用液状組成物の製造のための使用を提供する。Further, the present invention contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. And, the liquid composition having a pH of 3.5 or less is provided for use for producing a liquid composition for sterilization or bacteriostatic.
Further, the present invention contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Also provided is the use of a liquid composition having a pH of 3.5 or less for producing a liquid composition for imparting antibacterial properties to a subject.
本発明の殺菌又は静菌用液状組成物は、液状であるため使用しやすく、また高い殺菌又は静菌効果を有しながらも苦味が少なく食品の風味に影響を与えにくいので、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有効である。また、本発明の液状組成物は、素材に適用すれば、該素材についた微生物を殺菌又は静菌することができ、さらにその後長時間にわたって、該素材における微生物の増殖を継続的に抑えることができる。したがって、本発明は、恒常的に微生物増殖抑制が望まれる分野、例えば、食品の製造、加工、保存等の分野において有効である。 The liquid composition for sterilization or bacteriostatic of the present invention is easy to use because it is liquid, and has a high sterilization or bactericidal effect but has less bitterness and does not easily affect the flavor of food. It is effective for sterilization or bacteriostatic of the equipment that handles the equipment or the environment in which it exists. Further, when applied to a material, the liquid composition of the present invention can sterilize or sterilize microorganisms attached to the material, and can continuously suppress the growth of microorganisms in the material for a long period of time thereafter. can. Therefore, the present invention is effective in fields where constant suppression of microbial growth is desired, for example, in fields such as food production, processing, and storage.
本発明は、殺菌又は静菌用組成物を提供する。本発明の殺菌又は静菌用組成物は、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有する。 The present invention provides a bactericidal or bacteriostatic composition. The bactericidal or bacteriostatic composition of the present invention contains at least one selected from caprylic acid monoglyceride and capric acid monoglyceride, and ultraphosphate.
カプリル酸モノグリセリド及びカプリン酸モノグリセリドはいずれも、従来、乳化剤として食品等に適用されている物質であり、市販されている。例えば、カプリル酸モノグリセリドは、サンソフト（登録商標）７００ｐ－２（太陽化学株式会社）、ポエムＭ－１００（理研ビタミン株式会社）など、カプリン酸モノグリセリドは、サンソフト（登録商標）７６０（太陽化学株式会社）、ポエムＭ－２００（理研ビタミン株式会社）などの商品名で販売されている。 Both caprylic acid monoglyceride and caprylic acid monoglyceride are substances that have been conventionally applied to foods and the like as emulsifiers and are commercially available. For example, capric acid monoglyceride is Sunsoft (registered trademark) 700p-2 (Taiyo Kagaku Co., Ltd.), Poem M-100 (RIKEN Vitamin Co., Ltd.), and capric acid monoglyceride is Sunsoft (registered trademark) 760 (Taiyo Kagaku). Co., Ltd.), Poem M-200 (Riken Vitamin Co., Ltd.), etc.
本発明において、カプリル酸モノグリセリドとカプリン酸モノグリセリドは、いずれか一方を用いてもよいが、組み合わせて使用してもよい。本発明の組成物におけるカプリル酸モノグリセリドとカプリン酸モノグリセリドの合計濃度は、該組成物全量中、好ましくは０．０５質量％以上、さらに好ましくは０．１質量％以上が望ましい。この濃度が低いと、本発明の組成物は充分な殺菌又は静菌特性を得られない。一方、溶解性、経済性の観点から、本発明の組成物におけるカプリル酸モノグリセリドとカプリン酸モノグリセリドの合計濃度は、該組成物全量中、好ましくは１０．０質量％以下、より好ましくは２．０質量％以下、さらに好ましくは１．０質量％以下、なお好ましくは０．５％質量以下である。よって、本発明の組成物におけるカプリル酸モノグリセリドとカプリン酸モノグリセリドの合計濃度の範囲の例としては、該組成物全量中、好ましくは０．０５～１０．０質量％、より好ましくは０．０５～２．０質量％、さらに好ましくは０．０５～１．０質量％、さらに好ましくは０．０５～０．５質量％、さらに好ましくは０．１～０．５質量％が挙げられる。 In the present invention, either caprylic acid monoglyceride or caprylic acid monoglyceride may be used, or may be used in combination. The total concentration of caprylic acid monoglyceride and caprylic acid monoglyceride in the composition of the present invention is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, based on the total amount of the composition. If this concentration is low, the composition of the present invention will not have sufficient bactericidal or bacteriostatic properties. On the other hand, from the viewpoint of solubility and economic efficiency, the total concentration of caprylic acid monoglyceride and caprylic acid monoglyceride in the composition of the present invention is preferably 10.0% by mass or less, more preferably 2.0, based on the total amount of the composition. It is mass% or less, more preferably 1.0 mass% or less, still more preferably 0.5% mass or less. Therefore, as an example of the range of the total concentration of caprylic acid monoglyceride and caprylic acid monoglyceride in the composition of the present invention, the total amount of the composition is preferably 0.05 to 10.0% by mass, more preferably 0.05 to 0.05. 2.0% by mass, more preferably 0.05 to 1.0% by mass, still more preferably 0.05 to 0.5% by mass, still more preferably 0.1 to 0.5% by mass.
ウルトラリン酸は、酸性ヘキサメタリン酸とも称される。本発明で使用されるウルトラリン酸塩としては、ウルトラリン酸ナトリウムが好ましい。ウルトラリン酸ナトリウムは、従来、結着剤、ｐＨ調整剤等として食品等に適用されている。ウルトラリン酸塩は、市販されており、例えばウルトラポリホス（株式会社ポリホス化学研究所）、ウルトラポリン（太平化学産業株式会社）などの商品名で販売されている。 Ultraphosphoric acid is also referred to as acidic hexametaphosphoric acid. As the ultraphosphate used in the present invention, sodium ultraphosphate is preferable. Sodium ultraphosphate has been conventionally applied to foods and the like as a binder, a pH adjuster and the like. Ultraphosphate is commercially available, and is sold under trade names such as Ultrapolyphos (Polyphos Chemical Research Institute Co., Ltd.) and Ultraporin (Taipei Chemical Industry Co., Ltd.).
本発明の組成物におけるウルトラリン酸塩の濃度は、カプリル酸モノグリセリド又はカプリン酸モノグリセリドの濃度によっても異なり得るが、該組成物全量中、好ましくは０．０１５質量％以上、より好ましくは０．０１８質量％以上、さらに好ましくは０．０２質量％以上である。ウルトラリン酸塩の濃度が低いと、本発明の組成物が充分な殺菌又は静菌特性を得られない。他方、本発明の組成物におけるウルトラリン酸塩の濃度は、該組成物全量中、好ましくは３．８質量％以下、より好ましくは２．５質量％以下、さらに好ましくは１．０質量％以下である。ウルトラリン酸塩の濃度が高すぎると、本発明の組成物のｐＨが低くなり、金属の腐食など、本発明の組成物の適用環境に悪影響を及ぼすおそれがある。よって、本発明の組成物におけるウルトラリン酸塩の濃度の範囲の例としては、該組成物全量中、好ましくは０．０１５～３．８質量％、より好ましくは０．０１８～２．５質量％、さらに好ましくは０．０２～１．０質量％が挙げられる。 The concentration of ultraphosphate in the composition of the present invention may vary depending on the concentration of caprylic acid monoglyceride or caprylic acid monoglyceride, but is preferably 0.015% by mass or more, more preferably 0.018, based on the total amount of the composition. It is by mass or more, more preferably 0.02% by mass or more. If the concentration of ultraphosphate is low, the composition of the present invention will not have sufficient bactericidal or bacteriostatic properties. On the other hand, the concentration of ultraphosphate in the composition of the present invention is preferably 3.8% by mass or less, more preferably 2.5% by mass or less, still more preferably 1.0% by mass or less in the total amount of the composition. Is. If the concentration of ultraphosphate is too high, the pH of the composition of the present invention will be low, which may adversely affect the application environment of the composition of the present invention, such as metal corrosion. Therefore, as an example of the range of the concentration of ultraphosphate in the composition of the present invention, it is preferably 0.015 to 3.8% by mass, more preferably 0.018 to 2.5% by mass, based on the total amount of the composition. %, More preferably 0.02 to 1.0% by mass.
本発明の殺菌又は静菌用組成物に含まれ得るカプリル酸モノグリセリド、カプリン酸モノグリセリド、及びウルトラリン酸塩は、いずれも食品添加物として許容されている物質である。したがって、本発明の組成物は、一般的な洗浄剤や殺菌剤と比べて人体に対する安全性が高い。また、本発明の組成物は、従来の食品保存料や殺菌成分のような強い苦味がないため、食品とともに摂取しても、該食品の風味に影響しにくい。したがって、本発明の組成物は、食品関連分野への適用、例えば、食品、又はそれを取り扱う機器もしくはそれが存在する環境の殺菌又は静菌に有用である。 Caprylic acid monoglyceride, capric acid monoglyceride, and ultraphosphate, which can be contained in the sterilizing or bacteriostatic composition of the present invention, are all acceptable substances as food additives. Therefore, the composition of the present invention is highly safe for the human body as compared with general detergents and bactericides. Further, since the composition of the present invention does not have a strong bitterness unlike conventional food preservatives and bactericidal components, it does not easily affect the flavor of the food even when ingested together with the food. Therefore, the compositions of the present invention are useful for applications in food-related fields, such as sterilization or bacteriostatic of foods, or equipment that handles them or the environment in which they are located.
本発明の組成物は、カプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩以外の他の成分、例えば、ウルトラリン酸以外のリン酸（ポリリン酸、ピロリン酸、メタリン酸）、フィチン酸、クエン酸、グルコン酸、フマル酸、マレイン酸、マロン酸、酒石酸、コハク酸、リンゴ酸、アジピン酸、塩酸などの、ウルトラリン酸以外の有機酸及び無機酸、ならびにそれらの塩からなる群より選択される少なくとも１種、食品に使用可能なｐＨ調整剤、カプリル酸モノグリセリド及びカプリン酸モノグリセリド以外の脂肪酸エステルなどを含有していてもよい。ただし、これらの他の成分の種類及び含有量は、本発明の組成物に必要な殺菌又は静菌特性が失われない程度に制限される。成分の溶解性又は組成物の品質の安定性の観点からは、本発明の組成物は、Ｃ１１以上の鎖長の脂肪酸エステルを含有しないことが好ましい。カプリル酸モノグリセリド及びカプリン酸モノグリセリド以外の脂肪酸エステルの含有量としては５質量％以下が好ましい。より好ましくは、本発明の組成物における当該他の成分の含有量は、５質量％以下である。 The composition of the present invention comprises components other than capric acid monoglyceride, capric acid monoglyceride, and ultraphosphate, such as phosphates other than ultraphosphate (polyphosphate, pyrophosphate, metaphosphate), phytic acid, citric acid. , Gluconic acid, fumaric acid, maleic acid, malonic acid, tartaric acid, succinic acid, malic acid, adipic acid, hydrochloric acid and other organic and inorganic acids other than ultraphosphate, and salts thereof. It may contain at least one, a pH adjuster that can be used in foods, a fatty acid ester other than capric acid monoglyceride and capric acid monoglyceride, and the like. However, the types and contents of these other components are limited to the extent that the bactericidal or bacteriostatic properties required for the compositions of the present invention are not lost. From the viewpoint of the solubility of the components or the stability of the quality of the composition, it is preferable that the composition of the present invention does not contain a fatty acid ester having a chain length of C11 or more. The content of fatty acid esters other than caprylic acid monoglyceride and caprylic acid monoglyceride is preferably 5% by mass or less. More preferably, the content of the other component in the composition of the present invention is 5% by mass or less.
本発明の組成物は、さらにアルコール類を含有していてもよい。本発明の組成物に使用されるアルコール類としては、例えばエタノールなどの食品に使用可能なアルコール類が挙げられる。アルコール類の添加により、カプリル酸モノグリセリド又はカプリン酸モノグリセリドの溶解性が向上し、本発明の組成物の品質が安定する。本発明の組成物におけるアルコール類の含有量は、上述したカプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部以下であればよく、好ましくは該カプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部であり、より好ましくは、該組成物全量中２０～６０質量％である。 The composition of the present invention may further contain alcohols. Examples of the alcohols used in the composition of the present invention include alcohols that can be used in foods such as ethanol. The addition of alcohols improves the solubility of caprylic acid monoglyceride or capric acid monoglyceride, and stabilizes the quality of the composition of the present invention. The content of alcohols in the composition of the present invention may be less than or equal to the balance of the above-mentioned caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components, preferably the caprylic acid monoglyceride, capric acid monoglyceride, and the like. It is the balance of ultraphosphate and other components, more preferably 20-60% by weight in the total amount of the composition.
本発明の組成物は、さらに水性溶媒を含有していてもよい。好ましい水性溶媒としては、例えば水が挙げられる。本発明の組成物における該水性溶媒の含有量は、上述したカプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部以下であればよく、好ましくは該カプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩、他の成分及びアルコール類の残部であり、さらに好ましくは２０質量％以上である。 The composition of the present invention may further contain an aqueous solvent. Preferred aqueous solvents include, for example, water. The content of the aqueous solvent in the composition of the present invention may be less than or equal to the balance of the above-mentioned caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components, and preferably the caprylic acid monoglyceride and capric acid monoglyceride. , Ultraphosphate, other components and the balance of alcohols, more preferably 20% by mass or more.
本発明の組成物の一例は、上記水性溶媒に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解した液状組成物である。本発明の組成物の別の一例は、上記アルコール類に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解した液状組成物である。本発明の組成物の別の一例は、上記アルコール類と水性溶媒との混合溶媒に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解した液状組成物である。本発明の組成物の好ましい一例は、エタノール／水の混合溶媒に、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とが溶解したエタノール水溶液である。該エタノール／水の混合溶媒としては、２０～６０質量％エタノール水溶液が望ましい。該アルコール類と水性溶媒との混合溶媒、又は該エタノール／水の混合溶媒の本発明の組成物における含有量は、上述したカプリル酸モノグリセリド、カプリン酸モノグリセリド、ウルトラリン酸塩及び他の成分の残部であればよい。 An example of the composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in the above aqueous solvent. Another example of the composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in the above alcohols. Another example of the composition of the present invention is a liquid composition in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in a mixed solvent of the above alcohols and an aqueous solvent. Is. A preferred example of the composition of the present invention is an aqueous ethanol solution in which at least one selected from caprylic acid monoglyceride and capric acid monoglyceride and ultraphosphate are dissolved in a mixed solvent of ethanol / water. As the ethanol / water mixed solvent, a 20 to 60% by mass ethanol aqueous solution is desirable. The content of the mixed solvent of the alcohols and the aqueous solvent or the mixed solvent of ethanol / water in the composition of the present invention is the balance of the above-mentioned caprylic acid monoglyceride, capric acid monoglyceride, ultraphosphate and other components. It should be.
本発明の組成物のｐＨは、好ましくは３．５以下であり、より好ましくは３．０以下である。ｐＨが高くなると、本発明の組成物が充分な殺菌又は静菌特性を得られない。他方、本発明の組成物のｐＨは１．５以上が好ましい。ｐＨが低くなると、金属の腐食など、本発明の組成物の適用環境に悪影響を及ぼすおそれがある。よって、本発明の組成物のｐＨの範囲の例としては、好ましくは１．５～３．５、より好ましくは１．５～３．０が挙げられる。 The pH of the composition of the present invention is preferably 3.5 or less, more preferably 3.0 or less. When the pH is high, the composition of the present invention cannot obtain sufficient bactericidal or bacteriostatic properties. On the other hand, the pH of the composition of the present invention is preferably 1.5 or higher. When the pH is low, there is a risk of adversely affecting the application environment of the composition of the present invention, such as metal corrosion. Therefore, examples of the pH range of the composition of the present invention are preferably 1.5 to 3.5, more preferably 1.5 to 3.0.
本発明の組成物のｐＨは、主にウルトラリン酸塩の添加によって調整され得る。当該ｐＨ調整には、ウルトラリン酸塩以外の他の酸や、食品に使用可能なｐＨ調整剤を用いてもよい。 The pH of the composition of the present invention can be adjusted primarily by the addition of ultraphosphate. For the pH adjustment, an acid other than ultraphosphate or a pH adjusting agent that can be used for food may be used.
本発明の組成物は、優れた殺菌又は静菌作用を有しており、各種物質の殺菌又は静菌に使用することができる。本明細書において、殺菌とは、微生物を死滅させること（但し完全な死滅に限られない）をいい、また静菌とは、微生物の増殖を抑制することをいう。したがって、本発明の殺菌又は静菌用組成物を適用された対象においては、微生物の増殖が抑制されるか、又は微生物の数が減少する。 The composition of the present invention has an excellent bactericidal or bacteriostatic action, and can be used for sterilizing or bacteriostatic various substances. As used herein, sterilization means killing microorganisms (but not limited to complete killing), and bacteriostatic means suppressing the growth of microorganisms. Therefore, in the subject to which the bactericidal or bacteriostatic composition of the present invention is applied, the growth of microorganisms is suppressed or the number of microorganisms is reduced.
本発明の組成物により殺菌又は静菌される微生物の種類としては、特に限定されないが、例えば、Ｗｅｉｓｓｅｌｌａ属、Ｌｅｕｃｏｎｏｓｔｏｃ属等の乳酸菌、Ｋｌｅｂｓｉｅｌｌａ属、Ｅｎｔｅｒｏｂａｃｔｅｒ属等の大腸菌群、Ｃａｎｄｉｄａ属、Ｃｒｙｐｔｏｃｏｃｃｕｓ属等の酵母、Ｃｌａｄｏｓｐｏｒｉｕｍ属等のカビ、大腸菌（Ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ）、サルモネラ菌（Ｓａｌｍｏｎｅｌｌａ ｅｎｔｅｒｉｃａ）、黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ）、緑膿菌（Ｐｓｅｕｄｏｍｏｎａｓ ａｅｒｕｇｉｎｏｓａ）、リステリア菌（Ｌｉｓｔｅｒｉａ ｍｏｎｏｃｙｔｏｇｅｎｅｓ）、腸炎ビブリオ（Ｖｉｂｒｉｏ ｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ）、などが挙げられる。特に、乳酸菌等の耐酸性菌は、通常の微生物が生育を阻害される低ｐＨ領域でも生存するため従来除去しにくいものであったが、これらも本発明の組成物により殺菌又は静菌することができる。このように、本発明の組成物は、耐酸性菌等の従来除去しにくかった微生物種にも有用であるため、利用価値が高い。 The type of microorganism that is sterilized or bacteriostatic by the composition of the present invention is not particularly limited, and is, for example, lactic acid bacteria such as Weissella and Leuconostoc, Escherichia coli such as Klebsiella and Enterobacter, Candida, Cryptococcus and the like. Yeast, molds of the genus Cladosporium, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomona salis salis , And so on. In particular, acid-resistant bacteria such as lactic acid bacteria have been difficult to remove in the past because they survive even in a low pH region where normal microorganisms are inhibited from growing, but these are also sterilized or bacteriostatic by the composition of the present invention. Can be done. As described above, the composition of the present invention is also useful for microbial species that have been difficult to remove in the past, such as acid-resistant bacteria, and therefore has high utility value.
本発明の組成物は、各種素材の殺菌又は静菌に使用することができる。本発明の組成物の適用対象は、本発明の組成物との接触が許容されるものである限り、特に限定されない。本発明の組成物の適用対象の好ましい例としては、食品、食品を取り扱う機器、及び食品が存在する環境が挙げられる。該食品を取り扱う機器としては、製造、加工、流通、保存、調理等の過程で食品と接触する可能性のある機器、例えば、食品の製造もしくは加工工場の製造ラインの機器、食品の保存もしくは運搬用の容器、調理器具、調理台、作業者の手袋やエプロン、作業着、などが挙げられる。食品が存在する環境としては、食品を取り扱う環境、例えば食品の製造、加工、流通、保存、調理などが行われる環境、より詳細には、食品の製造もしくは加工工場、食品保管庫、調理場等の壁、床、天井、柱、ドア、窓、換気孔などが挙げられる。 The composition of the present invention can be used for sterilization or bacteriostatic of various materials. The application target of the composition of the present invention is not particularly limited as long as contact with the composition of the present invention is permitted. Preferred examples of the application target of the composition of the present invention include foods, devices for handling foods, and environments in which foods are present. Equipment that handles the food includes equipment that may come into contact with food in the process of manufacturing, processing, distribution, storage, cooking, etc., for example, equipment on the production line of a food manufacturing or processing factory, storage or transportation of food. Containers for foodstuffs, cooking utensils, cooking tables, worker gloves and aprons, work clothes, etc. The environment in which food exists includes an environment in which food is handled, for example, an environment in which food is manufactured, processed, distributed, stored, cooked, and more specifically, a food manufacturing or processing factory, a food storage, a kitchen, and the like. Walls, floors, ceilings, pillars, doors, windows, ventilation holes, etc.
本発明の殺菌又は静菌用組成物による対象の殺菌又は静菌は、該本発明の組成物を該対象に接触させることによって行われ得る。本発明の殺菌又は静菌用組成物は、液状であるため、殺菌又は静菌すべき対象への適用が容易である。例えば、本発明の液状組成物は、殺菌又は静菌対象の物質（例えば食品など）中に混合されてもよい。また例えば、本発明の液状組成物を、殺菌又は静菌対象の物質に噴霧もしくは塗布するか、又は本発明の液状組成物に該対象の物質を浸漬させてもよい。好ましくは、本発明の液状組成物は、殺菌又は静菌すべき対象に対して噴霧又は塗布される。本発明の液状組成物と接触した対象は、殺菌又は静菌される。 The sterilization or bacteriostatic of a subject by the sterilizing or bacteriostatic composition of the present invention can be carried out by bringing the composition of the present invention into contact with the subject. Since the sterilizing or bacteriostatic composition of the present invention is liquid, it can be easily applied to an object to be sterilized or bacteriostatic. For example, the liquid composition of the present invention may be mixed in a substance (for example, food) to be sterilized or bacteriostatic. Further, for example, the liquid composition of the present invention may be sprayed or applied to a substance to be sterilized or bacteriostatic, or the substance of the present invention may be immersed in the liquid composition of the present invention. Preferably, the liquid composition of the invention is sprayed or applied to an object to be sterilized or bacteriostatic. Objects in contact with the liquid composition of the present invention are sterilized or bacteriostatic.
本発明の好ましい態様においては、殺菌又は静菌すべき対象に対して本発明の組成物を噴霧もしくは塗布するか、又は浸漬させることで、該本発明の組成物と該対象とを接触させる。さらに、該本発明の組成物と接触させた対象を乾燥させてもよい。乾燥の方法としては、静置による乾燥、風乾、乾燥機による乾燥などが挙げられるが、特に限定されない。該乾燥により、本発明の組成物に含まれる揮発性成分、例えば水性溶媒やエタノールは、揮発して該対象から除去される。該揮発性成分が除去された後でも、本発明の組成物を適用した対象においては、殺菌又は静菌作用が持続する。したがって、本態様において、本発明の組成物は、液体の状態で対象に対して殺菌又は静菌力を発揮するだけでなく、乾燥物状態でも、該対象における微生物の増殖を抑える。 In a preferred embodiment of the invention, the composition of the invention is brought into contact with the subject by spraying, coating, or immersing the subject to be sterilized or bacteriostatic. Further, the subject in contact with the composition of the present invention may be dried. Examples of the drying method include drying by standing, air-drying, and drying by a dryer, but the drying method is not particularly limited. By the drying, volatile components contained in the composition of the present invention, such as an aqueous solvent and ethanol, are volatilized and removed from the subject. Even after the volatile components have been removed, the bactericidal or bacteriostatic action is sustained in the subject to which the composition of the invention is applied. Therefore, in the present embodiment, the composition of the present invention not only exerts bactericidal or bacteriostatic activity on a subject in a liquid state, but also suppresses the growth of microorganisms in the subject even in a dry state.
したがって、本発明の組成物の別の態様は、対象への抗菌性付与用液状組成物であり得る。本発明の組成物を、抗菌性を付与したい対象に噴霧、塗布、浸漬等により付着させることにより、該対象に抗菌性が付与される。必要に応じて又は好ましくは、さらに該本発明の組成物を付着させた対象を乾燥させる。本明細書において、対象の「抗菌性」とは、該対象の表面に付着又は内部に侵入した微生物を殺菌又は静菌することができるという、該対象の性質をいう。本態様は、恒常的に微生物増殖を防止したい環境、又はそこで使用される素材や機器、例えば、食品を取り扱う環境又はそこで使用される食品取り扱い用機器に対して、非常に有用である。 Therefore, another aspect of the composition of the present invention may be a liquid composition for imparting antibacterial activity to a subject. By adhering the composition of the present invention to an object to which the antibacterial property is desired by spraying, coating, dipping or the like, the antibacterial property is imparted to the object. If necessary or preferably, the subject to which the composition of the present invention is attached is further dried. As used herein, the term "antibacterial" of a subject refers to the property of the subject that it is capable of sterilizing or bacteriostatic microorganisms that have adhered to or invaded the surface of the subject. This embodiment is very useful for an environment in which microbial growth is to be constantly prevented, or materials and equipment used therein, for example, an environment in which food is handled or equipment for handling food used therein.
本発明の組成物は、対象の殺菌又は静菌に用いる場合、及び対象への抗菌性付与に用いる場合のいずれにおいても、対象の面積１００ｃｍ2あたり０．１～５．０ｍＬ程度の用量で使用されることが好ましく、対象の面積１００ｃｍ2あたり０．５～１．０ｍＬ程度の用量で使用されることがより好ましい。好ましくは、この用量の組成物を、対象に対して、好ましくは噴霧又は塗布により、均等に付着させる。The composition of the present invention is used at a dose of about 0.1 to 5.0 mL per 100 cm 2 of the target area regardless of whether it is used for sterilizing or bacteriostatic the subject or for imparting antibacterial properties to the subject. It is more preferable to use it at a dose of about 0.5 to 1.0 mL per 100 cm 2 of the target area. Preferably, this dose of the composition is evenly adhered to the subject, preferably by spraying or coating.
以下、実施例を挙げて、本発明をさらに詳細に説明するが、本発明はこれらの実施例にのみ限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.
参考例
室温下で、カプリル酸モノグリセリド又はカプリン酸モノグリセリドと精製水を混合して、表１に示す組成の液状組成物を調製した。得られた組成物の呈味性（苦味）を評価した。評価は、訓練を受けた１０名のパネルにより下記評価基準にて評価し、平均点を求めた。
（呈味性の評価基準）
５点：苦味がごくわずかで気にならない
４点：少しの苦味があるが許容できる
３点：苦味が感じられる
２点：苦味を感じ、口に入れると支障がある
１点：明らかな苦味があり、食べることに抵抗があるReference Example At room temperature, caprylic acid monoglyceride or capric acid monoglyceride and purified water were mixed to prepare a liquid composition having the composition shown in Table 1. The taste (bitter taste) of the obtained composition was evaluated. The evaluation was made by a panel of 10 trained people according to the following evaluation criteria, and the average score was calculated.
(Evaluation criteria for taste)
5 points: The bitterness is very slight and does not bother me 4 points: There is a slight bitterness but it is acceptable 3 points: The bitterness is felt 2 points: The bitterness is felt and there is a problem when it is put in the mouth 1 point: The obvious bitterness Yes, I'm reluctant to eat
結果を表１に示す。カプリル酸モノグリセリド又はカプリン酸モノグリセリドの濃度が上がるほど、組成物はより強い苦味を呈し、濃度０．１質量％以上では食用に許容できないほどの苦みを有していた。 The results are shown in Table 1. As the concentration of caprylic acid monoglyceride or capric acid monoglyceride increased, the composition exhibited a stronger bitterness, and at a concentration of 0.1% by mass or more, the bitterness was unacceptable for food.
試験例１
室温下で、カプリル酸モノグリセリド又はカプリン酸モノグリセリドと精製水を混合し、これに各種の酸を加えてｐＨを調整して、表２に示す組成の液状組成物を調製した。得られた組成物の呈味性（苦味）を、参考例と同様の基準で評価した。Test Example 1
At room temperature, caprylic acid monoglyceride or capric acid monoglyceride and purified water were mixed, and various acids were added thereto to adjust the pH to prepare a liquid composition having the composition shown in Table 2. The taste (bitter taste) of the obtained composition was evaluated based on the same criteria as in the reference example.
結果を表２～３に示す。ウルトラリン酸塩以外の酸を含む組成物は、苦味が強かった。また、ウルトラリン酸塩以外のリン酸塩を使用した場合、多量に使用しても組成物のｐＨが充分に下がらなかった。 The results are shown in Tables 2 and 3. Compositions containing acids other than ultraphosphate had a strong bitter taste. In addition, when a phosphate other than ultraphosphate was used, the pH of the composition did not drop sufficiently even when used in a large amount.
試験例２
室温下で、カプリル酸モノグリセリド又はカプリン酸モノグリセリド、エタノール及び滅菌済み精製水を１：２：２の割合で混合して溶液を調製し、これにウルトラリン酸塩又は他のリン酸塩、及び残余のエタノールと滅菌済み精製水を添加して表４～５記載の液状組成物（溶液）を得た。得られた各液状組成物について、下記の手順で殺菌又は静菌特性を評価した。Test Example 2
At room temperature, capric acid monoglyceride or capric acid monoglyceride, ethanol and sterilized purified water are mixed at a ratio of 1: 2: 2 to prepare a solution, which is then mixed with ultraphosphate or other phosphate and the residue. Ethanol and sterilized purified water were added to obtain the liquid compositions (solutions) shown in Tables 4 to 5. Each of the obtained liquid compositions was evaluated for sterilization or bacteriostatic properties by the following procedure.
〔殺菌又は静菌特性評価〕
（１）サンプル菌液の調製
対象菌には、４種の乳酸菌Ｗｅｉｓｓｅｌｌａ ｖｉｒｉｄｅｓｃｅｎｓ、Ｌｅｕｃｏｎｏｓｔｏｃ ｍｅｓｅｎｔｅｒｏｉｄｅｓ、Ｌｅｕｃｏｎｏｓｔｏｃ ｃｉｔｒｅｕｍ、及びＬｅｕｃｏｎｏｓｔｏｃ ｐｓｅｕｄｏｍｅｓｅｎｔｅｒｏｉｄｅｓを用いた。対象菌のシングルコロニーを白金耳を用いて数コロニー釣菌し、ＴＳＢ培地に植菌して３０℃で２０時間種培養したものを乳酸菌液（１０7ｃｆｕ／ｍＬ）とした。得られた乳酸菌液を、滅菌済み精製水１０ｍＬに０．１ｍＬ混合し、さらにこれを１０倍希釈した。４種の乳酸菌の１０倍希釈液を等量混合してサンプル菌液を調製した。[Sterilization or bacteriostatic property evaluation]
(1) Preparation of sample bacterial solution As the target bacteria, four kinds of lactic acid bacteria Weissella viridessens, Leuconostoc mastereoides, Leuconostoc citreum, and Leuconostoc pseudomesenteroides were used. A single colony of the target bacterium was cultivated in several colonies using a platinum loop, inoculated into a TSB medium, and seed-cultured at 30 ° C. for 20 hours to obtain a lactic acid bacterium solution ( 107 cfu / mL). The obtained lactic acid bacterium solution was mixed with 10 mL of sterilized purified water in an amount of 0.1 mL, and this was further diluted 10-fold. A sample bacterial solution was prepared by mixing equal amounts of 10-fold diluted solutions of 4 types of lactic acid bacteria.
（２）液状組成物の適用及び菌数測定
塗布直後及び１８時間後の液状組成物による静菌特性を調べた。滅菌プラスチックシャーレを二群に分けた。一方の群では、各液状組成物を１．０ｍＬずつ空のシャーレに入れ、コンラージ棒で均一に塗布し、次いでこれにサンプル菌液を１．０ｍＬ接種し、２０秒間コンラージ棒で混合しながら塗り広げた（０ｈ群）。もう一方の群では、各液状組成物を１．０ｍＬずつ空のシャーレに入れ、コンラージ棒で均一に塗布した後、安全キャビネット内で１８時間風乾し、水分とエタノールを完全に蒸発させた。これにサンプル菌液を１．０ｍＬ接種し、２０秒間コンラージ棒で混合しながら塗り広げた（１８ｈ群）。初発菌数は１０5～１０6ｃｆｕ/ｍＬに調整した。サンプル菌液を塗布したシャーレを、５分間静置後、シャーレ内の液体を採取し、ＭＲＳ寒天培地を用いて菌数を測定した。(2) Application of liquid composition and measurement of bacterial count The bacteriostatic characteristics of the liquid composition immediately after application and 18 hours after application were investigated. Sterilized plastic petri dishes were divided into two groups. In one group, 1.0 mL of each liquid composition was placed in an empty petri dish and spread evenly with a spreader, then 1.0 mL of sample bacterial solution was inoculated into this, and the mixture was spread with a spreader for 20 seconds. Spread out (0h group). In the other group, 1.0 mL of each liquid composition was placed in an empty petri dish, applied uniformly with a spreader, and then air-dried in a safety cabinet for 18 hours to completely evaporate water and ethanol. 1.0 mL of the sample bacterial solution was inoculated into this and spread while mixing with a spreader for 20 seconds (18h group). The initial bacterial count was adjusted to 10 5 to 10 6 cfu / mL. After allowing the petri dish coated with the sample bacterial solution to stand for 5 minutes, the liquid in the petri dish was collected and the bacterial count was measured using an MRS agar medium.
（３）殺菌又は静菌特性評価
（２）で測定した菌数をもとに、下記評価基準にて各組成物の殺菌又は静菌特性を評価した。
Ａ：初発の１／１０００以下に菌数が減少
Ｂ：初発の１／１００以下～１／１０００超に菌数が減少
Ｃ：初発の１／１０以下～１／１００超に菌数が減少
Ｄ：菌数が初発の１／１０よりも多い(3) Evaluation of sterilizing or bacteriostatic characteristics Based on the number of bacteria measured in (2), the sterilizing or bacteriostatic characteristics of each composition were evaluated according to the following evaluation criteria.
A: The number of bacteria decreases to 1/1000 or less of the first onset B: The number of bacteria decreases to 1/100 or less to more than 1/1000 of the first onset C: The number of bacteria decreases to 1/10 or less to more than 1/100 of the first onset D : The number of bacteria is higher than 1/10 of the first
結果を表４～５に示す。ウルトラリン酸塩以外のリン酸塩を含む組成物は、殺菌又は静菌作用を持続できなかった。 The results are shown in Tables 4-5. Compositions containing phosphates other than ultraphosphate could not sustain bactericidal or bacteriostatic action.
試験例３
試験例２と同様の手順で、ただしカプリル酸モノグリセリド又はカプリン酸モノグリセリドの濃度を変更して、表６～７記載の液状組成物を調製した。得られた組成物について、参考例と同様の手順で呈味性（苦味）を評価し、また試験例２と同様の手順で殺菌又は静菌特性を評価した。結果を表６～７に示す。カプリル酸モノグリセリド又はカプリン酸モノグリセリドを０．０５質量％以上含有する組成物は、苦味がなく、かつ持続的な殺菌又は静菌作用を有していた。Test Example 3
The liquid compositions shown in Tables 6 to 7 were prepared by the same procedure as in Test Example 2, but by changing the concentration of caprylic acid monoglyceride or caprylic acid monoglyceride. The obtained composition was evaluated for taste (bitter taste) by the same procedure as in Reference Example, and sterilized or bacteriostatic property was evaluated by the same procedure as in Test Example 2. The results are shown in Tables 6-7. The composition containing 0.05% by mass or more of caprylic acid monoglyceride or caprylic acid monoglyceride had no bitterness and had a continuous bactericidal or bacteriostatic effect.
試験例４
試験例２と同様の手順で、ただしウルトラリン酸塩の濃度を変更して、表８～９記載の液状組成物を調製し、試験例３と同様の手順で、呈味性、及び殺菌又は静菌特性を評価した。結果を表８～９に示す。ウルトラリン酸塩の濃度が低い組成物は苦味を有し、また持続的な殺菌又は静菌作用を示さなかった。Test Example 4
The liquid compositions shown in Tables 8 to 9 are prepared by the same procedure as in Test Example 2, but by changing the concentration of ultraphosphate, and the taste and sterilization or sterilization or sterilization is performed in the same procedure as in Test Example 3. The bacteriostatic properties were evaluated. The results are shown in Tables 8-9. Compositions with low concentrations of ultraphosphate had a bitter taste and did not show sustained bactericidal or bacteriostatic effects.
試験例５
室温下で、Ｃ８～Ｃ１２脂肪酸モノグリセリドと精製水を混合し、これにウルトラリン酸Ｎａを加えてｐＨを調整し、表１０に示す組成の液状組成物を調製した。得られた組成物の呈味性（苦味）及び液の均質さ（成分の溶解性）を評価した。呈味性については、参考例と同様の基準で評価した。液の均質さについては、調製直後又は冷蔵庫で一晩放置後の組成物について、成分の析出（白濁又は沈殿）の有無を目視で確認した。Test Example 5
At room temperature, C8 to C12 fatty acid monoglyceride and purified water were mixed, and Na ultraphosphate was added thereto to adjust the pH to prepare a liquid composition having the composition shown in Table 10. The taste (bitter taste) and the homogeneity of the liquid (solubility of the components) of the obtained composition were evaluated. The taste was evaluated based on the same criteria as in the reference example. Regarding the homogeneity of the liquid, the presence or absence of precipitation (white turbidity or precipitation) of the components was visually confirmed in the composition immediately after preparation or after being left in the refrigerator overnight.
結果を表１０に示す。カプリル酸（Ｃ８）モノグリセリド及びカプリン酸（Ｃ１０）モノグリセリドを含む組成物は、成分が水に容易に溶解して均質な溶液となったが、ラウリン酸（Ｃ１２）モノグリセリドを含む組成物は、成分が水に難溶であり、均質な溶液にはならなかった。 The results are shown in Table 10. The composition containing caprylic acid (C8) monoglyceride and capric acid (C10) monoglyceride had components easily dissolved in water to form a homogeneous solution, whereas the composition containing lauric acid (C12) monoglyceride had components. It was sparingly soluble in water and did not form a homogeneous solution.
試験例６
様々な素材上における液状組成物の殺菌又は静菌作用を調べた。
試験例２と同様の手順で、液状組成物を調製した（カプリル酸モノグリセリド ０．１５％、ウルトラリン酸Ｎａ ０．４０％、エタノール ４０．００％、水 残余；単位は質量％；ｐＨ２．４）。サンプル菌液は、試験例２と同様の手順で４種の菌を用いて調製した。
対象素材として、金属（ステンレス板）、ゴム（ゴム手袋）、プラスチック（番重ふた）を準備した。ステンレス板及び番重ふたは、１０ｃｍ四方に切断して使用した。ゴム手袋は、１０ｃｍ四方のプラスチック板に皺のないように伸ばして両面テープで貼り、余った部分はカットして使用した。Test Example 6
The bactericidal or bacteriostatic effects of liquid compositions on various materials were investigated.
A liquid composition was prepared in the same procedure as in Test Example 2 (caprylic acid monoglyceride 0.15%, ultraphosphate Na 0.40%, ethanol 40.00%, water residue; unit mass%; pH 2.4. ). The sample bacterial solution was prepared using four types of bacteria in the same procedure as in Test Example 2.
Metal (stainless steel plate), rubber (rubber gloves), and plastic (thick lid) were prepared as target materials. The stainless steel plate and the numbered lid were cut into 10 cm squares and used. The rubber gloves were stretched on a 10 cm square plastic plate without wrinkles and attached with double-sided tape, and the excess part was cut and used.
菌数の測定は、各素材につき以下の手順で行った。各対象素材を３群に分けた。第一の群では、液状組成物０．５ｍＬを、コンラージ棒で対象素材の上に均一に塗布し、次いでサンプル菌液０．１ｍＬを接種し、４０秒間コンラージ棒で混合しながら塗り広げた（０ｈ群）。残りの２群では、液状組成物０．５ｍＬ又は１．０ｍＬを、コンラージ棒で対象素材の上に均一に塗布した後、安全キャビネット内で１８時間風乾し、水分とエタノールを完全に蒸発させた。これにサンプル菌液１．０ｍＬを接種し、４０秒間コンラージ棒で混合しながら塗り広げた（１８ｈ群）。サンプル菌液の初発菌数は１０5～１０6ｃｆｕ/ｍＬに調整した。
サンプル菌液を塗布した対象素材を５分間静置後、無菌パウチに入れ、上から滅菌水１０ｍＬを加え、熱シールして上下に５回程度軽く振った。パウチを開封して中の液体を採取し、ＭＲＳ寒天培地で菌数を測定した。The number of bacteria was measured according to the following procedure for each material. Each target material was divided into 3 groups. In the first group, 0.5 mL of the liquid composition was evenly spread on the target material with a spreader, then 0.1 mL of the sample bacterial solution was inoculated and spread while mixing with the spreader for 40 seconds (. 0h group). In the remaining two groups, 0.5 mL or 1.0 mL of the liquid composition was evenly applied onto the target material with a spreader and then air dried in a safety cabinet for 18 hours to completely evaporate the water and ethanol. .. This was inoculated with 1.0 mL of the sample bacterial solution and spread while mixing with a spreader for 40 seconds (18h group). The initial bacterial count of the sample bacterial solution was adjusted to 105 to 10 6 cfu / mL.
The target material coated with the sample bacterial solution was allowed to stand for 5 minutes, placed in a sterile pouch, 10 mL of sterile water was added from above, heat-sealed, and shaken lightly up and down about 5 times. The pouch was opened, the liquid inside was collected, and the number of bacteria was measured on MRS agar medium.
結果を表１１に示す。本発明の組成物が、液状及び乾燥状態のいずれにおいても、各種素材の殺菌又は静菌に有効であることが分かった。 The results are shown in Table 11. It has been found that the composition of the present invention is effective for sterilizing or bacteriostatic various materials in both liquid and dry states.
Claims (12)
該液状組成物が付着した対象を乾燥させること、
を含み、
該液状組成物が、カプリル酸モノグリセリド及びカプリン酸モノグリセリドから選択される少なくとも１種と、ウルトラリン酸塩とを含有し、該カプリル酸モノグリセリド及びカプリン酸モノグリセリドの合計濃度が０．０５質量％以上であり、かつｐＨ３．５以下である、
対象への抗菌性付与方法。 To attach the liquid composition to the subject by spraying or applying the liquid composition to the subject, or by immersing the subject in the liquid composition, and
Drying the object to which the liquid composition is attached,
Including
The liquid composition contains at least one selected from caprylic acid monoglyceride and caprylic acid monoglyceride and ultraphosphate, and the total concentration of the caprylic acid monoglyceride and capric acid monoglyceride is 0.05% by mass or more. Yes, and the pH is 3.5 or less.
Method of imparting antibacterial properties to the subject.
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| JPH06153882A (en) * | 1992-07-13 | 1994-06-03 | Asama Kasei Kk | Preserving agent for food |
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