WO2018153702A1 - Utilisation d'une composition alimentaire pour animaux pour réduire les émissions de méthane chez les ruminants, et/ou améliorer le rendement des ruminants - Google Patents
Utilisation d'une composition alimentaire pour animaux pour réduire les émissions de méthane chez les ruminants, et/ou améliorer le rendement des ruminants Download PDFInfo
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- WO2018153702A1 WO2018153702A1 PCT/EP2018/053341 EP2018053341W WO2018153702A1 WO 2018153702 A1 WO2018153702 A1 WO 2018153702A1 EP 2018053341 W EP2018053341 W EP 2018053341W WO 2018153702 A1 WO2018153702 A1 WO 2018153702A1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/22—Methane [CH4], e.g. from rice paddies
Definitions
- the present invention relates to the field of reduction of methane emission in ruminants. Particularly, it relates to the use of a feed composition or a feed additive comprising at least one fatty acid containing at least 5 carbon atoms and at least one organic molecule substituted at any position with at least one nitrooxy group, for reducing the production of methane emanating from the digestive activities of ruminants, and/or to improve the ruminant performance.
- the present invention further relates to animal feed or animal feed compositions and feed additives comprising the above-mentioned molecules.
- feed or feed composition means any compound, preparation, mixture, or composition suitable for, or intended for intake by an animal.
- a ruminant is a mammal of the order Artiodactyla that digests plant- based food by initially softening it within the animal's first stomach, known as the rumen, then regurgitating the semi-digested mass, now known as cud, and chewing it again. The process of again chewing the cud to further break down plant matter and stimulate digestion is called "ruminating”.
- Methane is produced as a natural consequence of the anaerobic fermentation, which represents an energy loss to the host animal.
- Carbohydrate makes up 70 to 80 % of the dry matter in a typical dairy cattle ration and in spite of this the absorption of carbohydrates from the gastro-intestinal tract is normally very limited. The reason for this is the extensive fermentation of carbohydrates in the rumen resulting in production of acetate, propionate and butyrate as the main products. These products are part of the so called volatile fatty acids, (VFAs).
- VFAs volatile fatty acids
- methane is also a greenhouse gas, which is many times more potent than CO2. Its concentration in the atmosphere has doubled over the last century and continues to increase alarmingly. Ruminants are the major contributors to the biogenic methane formation, and it has been estimated that the prevention of methane formation from ruminants would almost stabilize atmospheric methane concentrations.
- Antibiotics and more particularly ionophores have been shown to slightly reduce methane production in ruminants (Guan et al. 2006. Journal of Animal Science; 84: 1896-1906).
- the effect of antibiotics on the formation of methane has some disadvantages because of rapid adaptation of the microflora and/or resistance development leading to a complete loss of the intended effect within a short period of time (2 to 3 weeks), and because the use of antibiotics is banned in Europe for non- therapeutic use.
- Non-antibiotic products leading to reduction of methane emission, when tested using an in vitro rumen simulation model, have recently been published (WO 2010/072584).
- WO 2010/072584 the amount required to produce a moderate reduction of methane emission are not compatible with the ruminant feed industry cost constraints.
- compositions specified herein after have a great potential for use in animal feed in order to essentially reduce the formation of methane without affecting microbial fermentation in a way that would be detrimental to the host animal.
- compositions of the present invention also have a great benefit regarding overall animal performance as measured by ruminal acetate/propionate ratio. Said compositions are also more stable than those described in the prior art, safer for the animal and human, and they are active at very low concentration in the rumen.
- the present invention provides the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) for reducing the formation of methane emanating from the digestive activities of ruminants and/or for improving ruminant performance.
- the invention further provides a method for reducing the production of methane emanating from the digestive activities of ruminants and/or for improving ruminant animal performance, comprising orally administering to the animal a sufficient amount of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) to the animal.
- a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) to the animal.
- organic molecules substituted at any position with at least one nitrooxy group, or salts thereof are defined by the following formula (I):
- a is comprised between 1 and 25, preferably between 1 and 10
- b is comprised between 2 and 51 , preferably between 2 and 21
- d is comprised between 0 and 8, preferably between 0 and 6
- e is comprised between 0 and 5, preferably between 0 and 3
- the organic molecule of formula (I) is of the following composition C a HbOdN e S g , wherein
- a is comprised between 1 and 10
- d is comprised between 0 and 6
- e is comprised between 0 and 3
- g is comprised between 0 and 1 ,
- preferred compounds of formula (I) according to the present inven- tion are compounds, wherein b is comprised between 3 and 51 , preferably, b is comprised between 3 and 21 .
- preferred compounds of formula (I) according to the present invention are compounds of formula (I I),
- n is comprised between 0 and 12, preferably comprised between 0 and 6 and, wherein, if n ⁇ 0, the carbon chain is a linear, a cyclic, or branched aliphatic carbon chain which may be non-substituted or substituted with up to 3 hydroxyl-, alkoxy-, amino-, alkyla- mino-, dialkylamino- or nitrooxy groups, or an alkenyl, or an alkynyl carbon chain mono- or polyunsaturated and in any isomeric form,
- R4 is independently, hydrogen or a saturated straight, cyclic or branched chain of an alkyl or alkenyl group containing 1 to 12, preferably 1 to 6 carbon atoms,
- X is hydrogen, R5, R5 ⁇ N, -OR5, -OCOR5, -NR5R6, -ON02, -COOR5, -CONR5R6, - NHS02R5, or -S02NHR5,
- R5 and R6 are independently, hydrogen, C1 -C12 straight, branched or cyclic alkyl chain, non-substituted or substituted with up to 3 hydroxyl-, alkoxy-, amino-, alkylamino-, dialkylamino- or nitrooxy groups, alkenyl, or alkynyl carbon chain which may be mono or polyunsaturated, and in any isomeric form.
- compounds of formula (I) and compounds of formula (II) can be in any isomeric form. It is to be understood in the above definition of compounds of formula (II) that when n > 2, the carbon chain can be linear or branched at any position along the carbon chain.
- the carbon chain can be branched by multiple branches at different positions along the carbon chain.
- the aliphatic carbon chain may form a cyclic moiety. This cyclic moiety can carry the nitrooxy moiety at any position (2, 3, 4), and it can also be branched at multiple positions by any aliphatic groups.
- the branched aliphatic groups are preferably, methyl, ethyl or propyl.
- the carbon chain may be further substituted with up to 3 hydroxyl-, alkoxy-, amino-, alkylamino-, dialkylamino- or nitrooxy groups.
- a preferred alkyl group is methyl, ethyl, propyl, isopropyl, butyl, sec. butyl, isobutyl, pentyl, neopentyl, hexyl, cyclohexyl, and 2-ethyl-hexyl and octyl.
- any alkyl or alkenyl group containing three or more carbon atoms can be straight chain, branched, or cyclic.
- more preferred compounds of formula (I) according to the present invention are selected from the list of compounds, and salts thereof as listed with their chemical formula in Table 1.
- even more preferred compounds of formula (I) are selected from the list of compounds, and salts thereof comprising 3-nitrooxypropanol, ethyl-3-nitrooxy propionate, methyl-3-nitrooxy propionate, and 3-nitrooxy propionic acid. Most preferred in all embodiments of the present invention is the use of 3-nitrooxypropanol.
- the compounds of formula (I) of the present invention also comprise salts of the nitrooxy organic molecule.
- Preferred cations for salt preparation may be selected from the group consisting of sodium (Na+), potassium (K+), lithium (Li+), magnesium (Mg2+), calcium (Ca2+), barium (Ba2+), strontium (Sr2+), and ammonium (NH4+). Salts may also be prepared from an alkali metal or an alkaline earth metal.
- the compounds of formula (I) according to the present invention can be manufactured in principle according to synthetic methods known per se for nitrooxy organic molecules, and/or based on methods as described in PCT/EP2010/069338, and in the European patent application No. 10 195 857.7.
- fatty acid containing at least 5 carbon atoms refers to saturated or unsaturated monocarboxylic acids of at least 5 carbon atoms, such as preferably to saturated or unsaturated monocarboxylic acids of 5 carbon atoms to 28 carbon atoms, more preferably of 7 carbon atoms to 21 carbon atoms, most preferably of 9 carbon atoms to 16 carbon atoms.
- Examples of such fatty acid include, but are not limited to, lauric acid, oleic acid, myristic acid, palmitic acid, myrisoleic acid, palmitoleic acid, linoleic acid and linolenic acids.
- the fatty acid is preferably a saturated monocarboxylic acid having the general formula CnH n+iCOOH, wherein n is an integer selected in the range of 4 to 27, preferably in the range of 6 to 20, more preferably in the range of 8 to 15, most preferably in the range of 9 to 13.
- n is an integer selected in the range of 4 to 27, preferably in the range of 6 to 20, more preferably in the range of 8 to 15, most preferably in the range of 9 to 13.
- lauric acid most preferably as sole fatty acid (i.e. in the absence of further fatty acids).
- the fatty acids may be used as such or in the form of a suitable salt thereof.
- Preferred cati- ons for salt preparation may be selected from the group consisting of sodium (Na+), potassium (K+), lithium (Li+), magnesium (Mg2+), calcium (Ca2+), barium (Ba2+), strontium (Sr2+), and ammonium (NH4+) such as in particular the respective alkaline or alkaline earth salt. Most preferably the respective sodium potassium or calcium salts.
- Methane emission by ruminants can easily be measured in individual animals in metabolic chambers by methods known in the art (Grainger et al., 2007 J. Dairy Science; 90: 2755- 2766).
- methane produced by a dairy ruminant can also be assessed by measurement of fatty acid profiles in milk according to WO 2009/156453.
- Ruminant performance can be assessed by methods well known in the art, and is often illustrated by the acetate proprionate ratio (mol ratio).
- the present invention also relates to the use of a feed composition or feed additive compris- ing at least one fatty acid containing at least 5 carbon atoms, and at least one organic mol- ecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) in combination with at least one additional active substance which shows similar effects with regard to methane formation in the rumen and which is selected from the group consisting of diallyl disulfide, garlic oil, allyl isothiocyanate, deoxycholic acid, chenodeoxycholic acid and derivatives thereof.
- yeasts for example yeasts, oregano extracts, and essential oils e.g. thymol, 3-methylphenol, vanillin, guaiacol and eugenol.
- essential oils e.g. thymol, 3-methylphenol, vanillin, guaiacol and eugenol.
- diallyl disulfide, garlic oil, allyl isothiocyanate deoxycholic acid, chenodeoxycholic acid and derivatives thereof are independently administered in dosage ranges of for example 0.01 -500 mg active substance per kg feed (ppm).
- ppm active substance per kg feed
- Ruminating mammals include cattle, goats, sheep, giraffes, American Bison, European bison, yaks, water buffalo, deer, camels, alpacas, llamas, wildebeest, antelope, pronghorn, and nilgai.
- domestic cattle, sheep and goat are the more preferred species.
- most preferred species are domestic cattle.
- the term includes all races of domestic cattle, and all production kinds of cattle, in particular dairy cows and beef cattle.
- the present invention also relates to the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the methane production in ruminants is reduced by at least 10 % when measured in metabolic chambers and based on the treatment with the at least one organic molecule substituted at any position.
- the methane reduction is at least 15 %, more preferably, at least 20 %, even more preferably, at least 25 %, most preferably, at least 30 %.
- Alternative methane emission measurements may also be used like using a laser beam or for dairy ruminants, correlating methane production to the VFA profile in milk.
- the present invention also relates to the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the volatile fatty acid ratio of acetate to propionate is reduced by at least 10 % based on the treatment with the at least one organic molecule substituted at any position with at least one nitrooxy group.
- volatile fatty acid ratio is reduced by at least 15 %, more preferably, by at least 20 %.
- the present invention also relates to the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the amount of the organic molecule as defined in formula (I) administered to the ruminant animal is from 1 mg to 10 g per Kg of feed, preferably from 10 mg to 1 g per Kg of feed, more preferably, from 50 mg to 500 mg per Kg of feed, and the amount of the at least one fatty acid containing at least 5 carbon atoms administered to the ruminant animal is from 0.1 to 20 g per Kg of feed, preferably from 1 to 15 g per Kg of feed, most preferably from 1 to 10 g per Kg of feed.
- organic molecules substituted at any position with at least one nitrooxy group, or their salts thereof as defined by formula (I) need not be that pure; it may e
- the present invention further relates to the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the mol ratio of the organic molecule as defined in formula (I) to the at least one fatty acid containing at least 5 carbon atoms in the feed composition or feed additive is comprised between 0.00001 and 1 , preferably between 0.0001 and 0.5, more preferably between 0.0025 and 0.1 , most preferably in the range of 0.004 to 0.05. Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01 .
- Ruminant feed or feed additives may be prepared by methods known per se in the art of feed formulation and processing. Further aspects of the present invention are therefore formulations, i.e. feed additives and animal feed compositions containing compositions as herein above defined.
- the present invention therefore also relates to a feed composition or a feed additive com- prising at least one fatty acid containing at least 5 carbon atoms and at least a compound of formula (I) or a salt thereof.
- the composition is a mineral premix, a vitamin premix including vitamins and minerals or a bolus.
- the normal daily dosage of a composition according to the invention provided to an animal by feed intake depends upon the kind of animal and its condition. Normally this dosage should be in the range of from about 1 mg to about 100 g, preferably from about 1 g to about 50 g, more preferably, 5 g to 10g compound per kg of feed.
- composition comprising at least one fatty acid containing at least 5 carbon atoms and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) may be used in combination with conventional ingredients present in an animal feed composition (diet) such as calcium carbonates, electrolytes such as ammonium chloride, proteins such as soya bean meal, wheat, starch, sunflower meal, corn, meat and bone meal, amino acids, animal fat, vitamins and trace minerals.
- animal feed composition diet
- electrolytes such as ammonium chloride
- proteins such as soya bean meal, wheat, starch, sunflower meal, corn, meat and bone meal
- amino acids amino acids
- animal fat vitamins and trace minerals.
- compositions of the invention are the following:
- An animal feed additive comprising (a) at least one compound selected from table 1 and (b) at least one fatty acid containing at least 5 carbon atoms, (c) at least one fat-soluble vitamin, (d) at least one water-soluble vitamin, (e) at least one trace mineral, and/or (f) at least one macro mineral;
- An animal feed composition comprising at least one fatty acid containing at least 5 carbon atoms and at least one compound selected from table 1 and a crude protein content of 50 to 800 g/kg feed.
- the so-called premixes are examples of animal feed additives of the invention.
- a premix designates a preferably uniform mixture of one or more micro-ingredients with diluents and/or carrier. Premixes are used to facilitate uniform dispersion of micro-ingredients in a larger mix.
- the premix of the invention contains at least one fat-soluble vitamin, and/or at least one water soluble vitamin, and/or at least one trace mineral, and/or at least one macro mineral.
- the premix of the invention comprises the at least one compound according to the invention together with at least one additional component selected from the group consisting of fat-soluble vitamins, water- soluble vitamins, trace minerals, and macro minerals.
- the premix comprises the active ingredients of the invention together with at least one additional component selected from the group consisting of fat-soluble vitamins, water-soluble vitamins, and trace-minerals.
- fat-soluble vitamins are vitamin A, vitamin D3, vitamin E, and vitamin K, e.g. vitamin K3.
- water-soluble vitamins are vitamin B12, biotin and choline, vitamin B1 , vitamin B2, vitamin B6, niacin, folic acid and panthothenate, e.g. Ca-D-panthothenate.
- trace minerals are manganese, zinc, iron, copper, iodine, selenium, and cobalt.
- macro minerals are calcium, phosphorus and sodium.
- the ruminant diet is usually composed of an easily degradable fraction (named concentrate) and a fiber-rich less readily degradable fraction (named hay, forage, or roughage).
- Hay is made of dried grass, legume or whole cereals.
- Grasses include among others timothy, ryegrasses, fescues.
- Legumes include among others clover, lucerne or alfalfa, peas, beans and vetches.
- Whole cereals include among others barley, maize (corn), oat, sorghum.
- Other forage crops include sugarcane, kales, rapes, and cabbages.
- root crops such as turnips, swedes, mangles, fodder beet, and sugar beet (including sugar beet pulp and beet molasses) are used to feed ruminants.
- Still further crops are tubers such as potatoes, cassava and sweet potato.
- Silage is an ensiled version of the fiber-rich fraction (e.g. from grasses, legumes or whole cereals) whereby material with a high water content is treated with a controlled anaerobic fermentation process (naturally-fermented or additive treated).
- the fiber-rich fraction e.g. from grasses, legumes or whole cereals
- material with a high water content is treated with a controlled anaerobic fermentation process (naturally-fermented or additive treated).
- Concentrate is largely made up of cereals (such as barley including brewers grain and distillers grain, maize, wheat, sorghum), but also often contain protein-rich feed ingredients such as soybean, rapeseed, palm kernel, cotton seed and sunflower.
- Cows may also be fed total mixed rations (TMR), where all the dietary components, e.g. forage, silage and concentrate, are mixed before serving.
- TMR total mixed rations
- a premix is an example of a feed additive which may comprise the active compounds according to the invention. It is understood that the compounds may be administered to the animal in different other forms. For example, the compounds can also be included in a bolus that would be placed in the rumen and that would release a defined amount of the active compounds continuously in well defined dosages over a specific period of time.
- the present invention further relates to a method for reducing the production of methane emanating from the digestive activities of ruminants and/or for improving ruminant animal performance, comprising orally administering a sufficient amount of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) with the preferred embodiments described above.
- the invention further relates to a method as described above, wherein the feed composition or feed additive according to the present invention is administered to the animal in combination with at least one additional active substance selected from the group consisting of diallyl disulfide, garlic oil, allyl isothiocyanate, deoxycholic acid, chenodeoxy- cholic acid and derivatives thereof.
- at least one additional active substance selected from the group consisting of diallyl disulfide, garlic oil, allyl isothiocyanate, deoxycholic acid, chenodeoxy- cholic acid and derivatives thereof.
- the invention also relates to a method as described above, wherein the ruminant animal is selected from the group consisting of: cattle, goats, sheep, giraffes, American Bison, European bison, yaks, water buffalo, deer, camels, alpacas, llamas, wildebeest, antelope, pronghorn, and nilgai, and more preferably from the group consisting of: cattle, goats and sheep.
- the invention also relates to a method as described above, wherein the amount of the at least one organic molecule as defined in formula (I) administered to the ruminant animal is from about 1 mg to about 10 g per kg feed, preferably from about 10 mg to about 1 g, more preferably from 50 mg to 500 mg compound per kg of feed, and the amount of the at least one fatty acid containing at least 5 carbon atoms administered to the ruminant animal is from 0.1 to 52 g per Kg of feed, preferably from 1 to 15 g per Kg of feed, most preferably from 1 to 10 g per Kg of feed.
- the invention also relates to a method as described above, wherein the methane production in ruminants is reduced by at least 10 % when measured in metabolic chambers and based on the treatment with the at least one organic molecule substituted at any position.
- the methane reduction is at least 15 %, more preferably, at least 20 %, even more preferably, at least 25 %, most preferably, at least 30 %.
- Alternative methane emis- sion measurements may also be used like using a laser beam or for dairy ruminants, correlating methane production to the VFA profile in milk.
- the invention also relates to a method as described above, wherein the volatile fatty acid ratio of acetate to propionate (mol ratio) is reduced by at least 10 % based on the treatment with the at least one organic molecule substituted at any position with at least one nitrooxy group.
- volatile fatty acid ratio is reduced by at least 15 %, more preferably, by at least 20 %.
- the invention also relates to a method as described above, wherein the mol ratio of organic molecule as defined in formula (I) to at least one fatty acid containing at least 5 carbon atoms in the feed composition or feed additive is comprised between 0.00001 and 1 , preferably between 0.0001 and 0.5, more preferably between 0.0025 and 0.1 , most preferably in the range of 0.004 to 0.01 . Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01 .
- the present invention relates to the use of a mixture of 3-nitrooxypropanol and lauric acid to reduce the methane formation in ruminants and/ or to decrease the acetate/ propionate ratio (mol-ratio) in ruminants.
- the mole-ratio of 3-nitrooxypropanol to lauric acid in the mixture is selected in the range of 0.00001 to 1 , preferably in the range of 0.0001 to 0.5, more preferably in the range of 0.0025 to 0.1 , most preferably in the range of 0.004 to 0.01 . Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01.
- the methane reduction is at least 15 %, more preferably, at least 20 %, even more preferably, at least 25 %, most preferably, at least 30 % based on the administration of 3-nitrooxypropanol alone (measured in metabolic chambers) and/ or the decrease in the acetate propionate ratio is at least 3%, more preferably at least 4 % based on the administration of 3- nitrooxypropanol alone.
- the invention in another embodiment relates to a reduction of the methane formation and/or decrease in the acetate/ propionate ratio (mol-ratio) in ruminants said method encompassing the step of administering to a ruminant a mixture of 3-nitrooxypropnaol and lauric acid.
- the mole-ratio of 3-nitrooxypropanol to lauric acid in the mixture is selected in the range of 0.00001 to 1 , preferably in the range of 0.0001 to 0.5, more pref- erably in the range of 0.0025 to 0.1 , most preferably in the range of 0.004 to 0.01 . Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01 .
- the methane reduction is at least reduction is at least 15 %, more preferably, at least 20 %, even more preferably, at least 25 %, most preferably, at least 30 % based on the administration of 3-nitrooxypropanol alone (measured in metabolic chambers) and/ or the de- crease in the acetate propionate ratio is at least 3%, more preferably at least 4 % based on the administration of 3-nitrooxypropanol alone.
- HFT Hohenheim Forage value Test
- Feed is gadded into a syringe with a composition of rumen liquor and an appropriate mixture of buffers. The solution is incubated at 39°C. After 8 hours the quantity (and composition) of gas phase produced is measured and put into a formula for conversion.
- Reagents :
- KH2PO4 potassium dihydrogen phosphate
- Sample weighing The feed stuff is sieved to 1 mm - usually TMR (44 % concentrate, 6 % hay, 37 % maize silage and 13 % grass silage) - and weighed exactly into 64 syringes. 4 of these syringes are the substrate controls, which display the gas production without the ef- feet of the tested compounds. 4 other syringes are positive control, in which bromoethane sulfonate has been added to 0.1 mM. When needed, 4 syringes contain a carrier control (if the test compounds need a carrier). The remaining syringes contain the test substances, by groups of 4 syringes.
- TMR 44 % concentrate, 6 % hay, 37 % maize silage and 13 % grass silage
- the components are mixed in a Woulff bottle in following order:
- the completed solution is warmed up to 39 °C followed by the addition of 1 .83 ml sodium salt solution and the addition of reduction solution at 36 °C.
- the rumen liquor is added, when the indicator turns colourless.
- Extraction of the rumen liquor 750 ml of rumen liquor are added to approximately 1 ,400 ml of medium solution under continued agitation and C02-gassing.
- the diluted rumen fluid (24 ml) is added to the glass syringe.
- the syringes are then incubated for 8 hours at 39 °C under gentle agitation. After 8 hours, the volume of gas produced is measured, and the percentage of methane in the gas phase is determined by gas chromatography.
- the food fermented was artificial TMR (44 % concentrate, 6 % hay, 37 % maize silage and 13 % grass silage).
- Laurie acid from Sigma Aldrich was used at a concentration of 1000 ⁇ .
- 3-Nitrooxy-propanol (3-NOP) was used at two different concentrations, 2.5 ⁇ and 5 ⁇ .
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Abstract
Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP18705867.2A EP3585182B1 (fr) | 2017-02-21 | 2018-02-09 | Utilisation d'une composition alimentaire pour animaux pour réduire les émissions de méthane chez les ruminants, et/ou améliorer le rendement des ruminants |
KR1020197027364A KR20190112329A (ko) | 2017-02-21 | 2018-02-09 | 반추동물에서의 메탄 배출의 감소 및/또는 반추동물 성능의 개선을 위한 사료 조성물의 용도 |
BR112019017258-0A BR112019017258B1 (pt) | 2017-02-21 | 2018-02-09 | Composição de ração ou aditivo de ração, seu uso e método para reduzir a produção de metano proveniente das atividades digestivas de ruminantes |
US16/486,974 US11191288B2 (en) | 2017-02-21 | 2018-02-09 | Use of a feed composition for reducing methane emission in ruminants, and/or to improve ruminant performance |
PL18705867T PL3585182T3 (pl) | 2017-02-21 | 2018-02-09 | Wykorzystanie kompozycji paszowych do redukcji emisji metanu u przeżuwaczy i/lub poprawy wydajności przeżuwaczy |
JP2019544868A JP7019925B2 (ja) | 2017-02-21 | 2018-02-09 | 反芻動物のメタン排出量を減少させるための、且つ/又は反芻動物のパフォーマンスを改善するための飼料組成物の使用 |
AU2018226085A AU2018226085B2 (en) | 2017-02-21 | 2018-02-09 | Use of a feed composition for reducing methane emission in rumi-nants, and/or to improve ruminant performance |
DK18705867.2T DK3585182T3 (da) | 2017-02-21 | 2018-02-09 | Anvendelse af en fodersammensætning til reducering af methanudslip fra drøvtyggere og/eller til forbedring af drøvtyggeres ydeevne |
CN201880013070.7A CN110312436B (zh) | 2017-02-21 | 2018-02-09 | 饲料组合物来减少反刍动物中甲烷排放和/或改善反刍动物性能的用途 |
NZ756537A NZ756537B2 (en) | 2017-02-21 | 2018-02-09 | Use of a feed composition for reducing methane emission in rumi-nants, and/or to improve ruminant performance |
ES18705867T ES2882287T3 (es) | 2017-02-21 | 2018-02-09 | Uso de una composición de pienso para reducir la emisión de metano en rumiantes y/o mejorar el rendimiento de los rumiantes |
MX2019009894A MX2019009894A (es) | 2017-02-21 | 2018-02-09 | Uso de una composición alimenticia para reducir la emisión de metano en ruminantes, y para mejorar el rendimiento de los mismos. |
CA3053865A CA3053865A1 (fr) | 2017-02-21 | 2018-02-09 | Utilisation d'une composition alimentaire pour animaux pour reduire les emissions de methane chez les ruminants, et/ou ameliorer le rendement des ruminants |
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