NZ756537B2 - Use of a feed composition for reducing methane emission in rumi-nants, and/or to improve ruminant performance - Google Patents
Use of a feed composition for reducing methane emission in rumi-nants, and/or to improve ruminant performanceInfo
- Publication number
- NZ756537B2 NZ756537B2 NZ756537A NZ75653718A NZ756537B2 NZ 756537 B2 NZ756537 B2 NZ 756537B2 NZ 756537 A NZ756537 A NZ 756537A NZ 75653718 A NZ75653718 A NZ 75653718A NZ 756537 B2 NZ756537 B2 NZ 756537B2
- Authority
- NZ
- New Zealand
- Prior art keywords
- feed
- per
- nitrooxypropanol
- lauric acid
- ruminant animal
- Prior art date
Links
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- 239000000203 mixture Substances 0.000 title claims abstract description 53
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- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
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- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 1
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- 229940117960 vanillin Drugs 0.000 description 1
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 235000012141 vanillin Nutrition 0.000 description 1
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- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
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- 239000011647 vitamin D3 Substances 0.000 description 1
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/22—Methane [CH4], e.g. from rice paddies
Abstract
The present invention relates to the field of reduction of methane emission in ruminants. Particularly, it relates to the use of a feed composition or a feed additive comprising lauric acid and 3-nitrooxypropanol in a ratio of between 0.0025 and 0.1 to 1, for reducing the production of methane emanating from the digestive activities of ruminants, and/or to improve the ruminant performance. ting from the digestive activities of ruminants, and/or to improve the ruminant performance.
Description
USE OF A FEED COMPOSITION FOR REDUCING METHANE EMISSION IN RUMINANTS , AND/OR TO IMPROVE RUMINANT PERFORMANCE The present invention relates to the field of reduction of methane emission in ruminants.
Particularly, it relates to the use of a feed composition or a feed additive comprising at least one fatty acid containing at least 5 carbon atoms and at least one c le substituted at any position with at least one nitrooxy group, for reducing the production of methane emanating from the digestive activities of ruminants, and/or to improve the ruminant performance.
The present invention further relates to animal feed or animal feed compositions and feed ves comprising the above-mentioned molecules. The term feed or feed composition means any compound, preparation, mixture, or composition suitable for, or intended for intake by an animal.
In the present context, a ruminant is a mammal of the order Artiodactyla that digests plantbased food by initially softening it within the animal's first stomach, known as the rumen, then regurgitating the semi-digested mass, now known as cud, and g it again. The process of again chewing the cud to further break down plant matter and stimulate digestion is called "ruminating".
Rumen fermentation brings some disadvantages. Methane is ed as a natural consequence of the anaerobic fermentation, which represents an energy loss to the host animal.
Carbohydrate makes up 70 to 80 % of the dry matter in a typical dairy cattle ration and in spite of this the tion of carbohydrates from the gastro-intestinal tract is normally very limited. The reason for this is the extensive fermentation of carbohydrates in the rumen resulting in production of e, nate and butyrate as the main products. These products are part of the so called volatile fatty acids, .
Besides the energy loss, methane is also a greenhouse gas, which is many times more potent than CO2. Its concentration in the atmosphere has doubled over the last century and continues to increase alarmingly. Ruminants are the major contributors to the biogenic methane formation, and it has been estimated that the prevention of methane ion from nts would almost stabilize atmospheric methane concentrations.
Furthermore, the assessment of the Kyoto protocol followed by the Copenhagen climate summit in 2009 places sed priority in decreasing methane emissions as part of a multi-gas strategy.
Antibiotics and more particularly ionophores have been shown to slightly reduce methane production in ruminants (Guan et al. 2006. Journal of Animal Science; 84: 1896-1906).
However, the effect of antibiotics on the formation of methane has some disadvantages because of rapid adaptation of the lora and/or resistance development leading to a complete loss of the intended effect within a short period of time (2 to 3 weeks), and be- cause the use of antibiotics is banned in Europe for non- therapeutic use.
Non-antibiotic products (bile acid derivatives) g to reduction of methane on, when tested using an in vitro rumen simulation model, have recently been published (WO 72584). r, the amount required to produce a moderate reduction of methane emission are not compatible with the ruminant feed industry cost aints.
Furthermore, a number of natural plant extracts (Garlic: , rhubarb) have been described in the scientific literature as potent solutions to reduce methane emission in ruminants based on in vitro experiments. However, none of these so- lutions made it to a commercial product because of side effects (residues in milk), or because of the very large amount of additive which needs to be supplied to the animal to generate a significant e reduction.
Under these circumstances there is still a need to develop new solutions to significantly reduce the formation of methane produced by ruminants. In addition to reducing methane emission, such compositions should ably also contribute to improve ruminant performance by decreasing the e/propionate ratio.
The present inventors now surprisingly found that the composition specified herein after, have a great potential for use in animal feed in order to essentially reduce the formation of methane t ing microbial fermentation in a way that would be detrimental to the host animal. Moreover, the itions of the present invention also have a great benefit regarding overall animal performance as measured by ruminal acetate/propionate ratio.
Said compositions are also more stable than those described in the prior art, safer for the animal and human, and they are active at very low concentration in the rumen.
Therefore, the present invention provides the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms and at least one c molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) for reducing the formation of methane emanating from the digestive ties of ruminants and/or for improving ruminant performance.
The present invention further provides a use of a feed composition or feed additive comprising lauric acid and 3--nitrooxypropanol, for reducing the formation of methane emanating from the digestive activities of ruminants, wherein the mol ratio of 3 nitrooxypropanol to lau- ric acid is comprised between 0.0025 and 0.1 and the amount of 3-nitrooxypropanol administered to the ruminant animal is from 1 mg to 10 g per kg feed, and the amount of lauric acid administered to the ruminant animal is from 0.1 to 20 g per Kg of feed.
The t invention further provides a feed composition or feed additive comprising lauric acid and 3-nitrooxypropanol, characterized in that the mol ratio of 3-nitrooxypropanol to lauric acid is sed en 0.0025 and 0.1 and the amount of 3-nitrooxypropanol administered to the ruminant animal is from 1 mg to 10 g per kg feed, and the amount of lauric acid administered to the ruminant animal is from 0.1 to 20 g per Kg of feed.
The present invention further provides a ruminant animal feed composition or feed ve comprising lauric acid and 3-nitrooxypropanol, characterized in that the mol ratio of 3- nitrooxypropanol to lauric acid is comprised between 0.0025 and 0.1 and the composition or feed additive is d to administer 3-nitrooxypropanol to the ruminant animal in a range of from 1 mg to 10 g per kg feed, and the composition or feed additive is adapted to admin- ister lauric acid to the ruminant animal in a range of from 0.1 to 20 g per Kg of feed.
The invention further provides a method for reducing the production of methane ing from the digestive activities of ruminants and/or for improving ruminant animal performance, comprising orally administering to the animal a sufficient amount of a feed composition or feed ve comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt f as defined by formula (I) to the animal. It is to be understood by oral administration , a simple feeding, or manual stration of a bolus.
The invention further provides a method for reducing the tion of methane emanating from the digestive activities of ruminants comprising orally administering to the animal a ient amount of a feed composition or feed additive comprising at least lauric acid, and at least 3-nitrooxypropanol, characterized in that the mol ratio of 3-nitrooxypropanol to lauric acid is comprised between 0.0025 and 0.1 and the amount of 3-nitrooxypropanol stered to the ruminant animal is from 1 mg to 10 g per kg feed, and the amount of lauric acid administered to the ruminant animal is from 0.1 to 20 g per Kg of feed.
In all embodiments of the present invention, organic molecules substituted at any position with at least one nitrooxy group, or salts thereof are defined by the following a (I): 2 O Y formula (I) wherein Y is an organic le of the following composition: CaHbOdNeSg, wherein a is comprised between 1 and 25, preferably between 1 and 10 b is sed between 2 and 51, preferably between 2 and 21 d is comprised between 0 and 8, preferably between 0 and 6 e is comprised between 0 and 5, preferably between 0 and 3 g is comprised between 0 and 3, preferably between 0 and 1.
More ably, in all embodiments of the t invention, the organic molecule of formula (I) is of the following composition CaHbOdNeSg, wherein a is comprised between 1 and 10 b is comprised n 2 and 21 d is comprised between 0 and 6 e is comprised between 0 and 3 g is comprised between 0 and 1, In another embodiment, preferred compounds of a (I) according to the present invention are compounds, wherein b is comprised between 3 and 51, preferably, b is comprised between 3 and 21.
In another embodiment, preferred compounds of a (I) according to the present invention are compounds of formula (II), R4 n formula (II) wherein n is comprised n 0 and 12, preferably sed between 0 and 6 and, wherein, if n ≠ 0, the carbon chain is a linear, a cyclic, or branched aliphatic carbon chain which may be bstituted or substituted with up to 3 hydroxyl-, -, amino-, alkylamino- , dialkylamino- or nitrooxy groups, or an alkenyl, or an alkynyl carbon chain mono- or polyunsaturated and in any isomeric form, R4 is independently, hydrogen or a ted straight, cyclic or branched chain of an alkyl or alkenyl group containing 1 to 12, preferably 1 to 6 carbon atoms, X is hydrogen, R5, R5≡N, -OR5, -OCOR5, -NR5R6, -ONO2, -COOR5, -CONR5R6, - NHSO2R5, or -SO2NHR5, R5 and R6 are independently, hydrogen, C1-C12 straight, branched or cyclic alkyl chain, non-substituted or substituted with up to 3 hydroxyl-, alkoxy-, amino-, alkylamino-, di- alkylamino- or nitrooxy groups, alkenyl, or alkynyl carbon chain which may be mono or polyunsaturated, and in any isomeric form.
For all embodiments of the present ion, it is to be understood that compounds of formula (I) and compounds of formula (II) can be in any isomeric form.
It is to be tood in the above tion of compounds of formula (II) that when n > 2, the carbon chain can be linear or branched at any position along the carbon chain. In addition , the carbon chain can be branched by le branches at different positions along the carbon chain. Moreover, when n > 3, the aliphatic c arbon chain may form a cyclic moiety.
This cyclic moiety can carry the nitrooxy moiety at any position (2, 3, 4), and it can also be branched at multiple ons by any tic groups. The branched aliphatic groups are preferably, methyl, ethyl or propyl. Moreover, the carbon chain may be further substituted with up to 3 hydroxyl-, alkoxy-, amino-, alkylamino-, dialkylamino- or nitrooxy groups.
In the above definition of derivatives of the formula (II) a preferred alkyl group is methyl, ethyl, propyl, isopropyl, butyl, sec. butyl, isobutyl, pentyl, neopentyl, hexyl, cyclohexyl, and 2-ethyl-hexyl and octyl. Furthermore, any alkyl or alkenyl group containing three or more carbon atoms can be straight chain, ed, or cyclic. In addition for the straight chain or branched C2-C10-alkenylene group, this is understood to ass alkenylene groups with one or (from C4) more double bonds; examples of such alkenylene groups are those of the formulae -CH=CH-, -CH=CH-CH2-, -CH=CH-(CH2)3- and -(CH=CH)2-.
In another embodiment, more preferred compounds of formula (I) according to the present invention are selected from the list of compounds, and salts thereof as listed with their chemical formula in Table 1.
Table 1: Preferred nds of formula (I) according to the present invention Comp.
Molecular structure Chemical name Identifier 1 NO 3-Nitrooxypropanol OH O 2 NO racPhenylbutane-1,2- 2 O 2 diyl ate 2 2-(Hydroxymethyl) 3 (nitrooxymethyl)-1,3- HO OH propanediol 4 H N-Ethylnitro-oxy- N NO2 O propionic sulfonyl amide N 5-Nitrooxy-pentanenitrile 2 O 6 O2N 5-Nitrooxy-pentane O o-oxy-propyl propio- 2 nate O O 8 O N NO 2 1,3-bis-Nitrooxypropane O O 2 9 O2N O O NO2 1,4-bis-Nitrooxybutane O2N NO2 1,5-bis-Nitrooxypentane O O 3-Nitro-oxy-propyl benzo- 11 O O NO 2 ate O 3-Nitro-oxy-propyl hexano- O O NO2 ate O 3-Nitro-oxy-propyl 5-nitro- O N NO 2 oxy-hexanoate O O O 2 O 2 14 Benzylnitrate O N O 2 H O O isosorbid-dinitrate O N-[2-(Nitrooxy)ethyl] N NO 2 pyridinecarboxamide 17 O2N ooxy propionic acid O OH methylnitrooxy 18 O2N O O propionate 19 O N Ethylnitrooxy propionate 2 O O O Ethylnitrooxy butanoate O N O O O 21 Ethylnitrooxy butanoate 22 O N 5-nitrooxy pentanoic acid 2 O OH -nitrooxy 23 O N 2 O O pentanoate 24 O ooxy hexanoic acid O2N OH O Ethylnitrooxy hexanoate 2 O 26 O ethylnitrooxy- cyclohexylcarboxylate 27 O 8-nitrooxy octanoic acid O N OH 28 O Ethylnitrooxy octanoate 2 O O 11-nitrooxy undecanoic 29 O2N O OH acid O Ethylnitrooxy O2N O O undecanoate -nitrooxy-pentanoic 31 O N 2 O NH amide -nitrooxy-N-methyl- 32 O2N O N pentanoic amide In another embodiment, even more preferred compounds of formula (I) are selected from the list of compounds, and salts thereof comprising 3-nitrooxypropanol, ethylnitrooxy nate, methylnitrooxy propionate, and 3-nitrooxy propionic acid. Most preferred in all embodiments of the present invention is the use of 3-nitrooxypropanol.
The nds of formula (I) of the present invention also comprise salts of the nitrooxy organic molecule. Preferred cations for salt preparation may be selected from the group consisting of sodium (Na+), potassium (K+), lithium (Li+), magnesium (Mg2+), calcium (Ca2+), barium (Ba2+), strontium , and um (NH4+). Salts may also be prepared from an alkali metal or an alkaline earth metal.
The compounds of formula (I) according to the present invention can be manufactured in principle according to synthetic methods known per se for nitrooxy organic molecules, and/or based on methods as described in 2010/069338, and in the European patent application No. 10 195 857.7.
The term fatty acid containing at least 5 carbon atoms as used herein refers to saturated or unsaturated monocarboxylic acids of at least 5 carbon atoms, such as preferably to saturated or unsaturated monocarboxylic acids of 5 carbon atoms to 28 carbon atoms, more pref- erably of 7 carbon atoms to 21 carbon atoms, most ably of 9 carbon atoms to 16 carbon atoms. es of such fatty acid include, but are not limited to, lauric acid, oleic acid, myristic acid, palmitic acid, myrisoleic acid, palmitoleic acid, linoleic acid and linolenic acids.
In all embodiments of the present invention, the fatty acid is preferably a saturated monocarboxylic acid having the general formula CnH2n+1COOH, wherein n is an integer selected in the range of 4 to 27, preferably in the range of 6 to 20, more preferably in the range of 8 to 15, most ably in the range of 9 to 13. Most preferred in all embodiments of the present invention is the use lauric acid, most preferably as sole fatty acid (i.e. in the absence of r fatty acids).
The fatty acids may be used as such or in the form of a le salt thereof. Preferred cations for salt ation may be selected from the group consisting of sodium (Na+), potassium (K+), lithium (Li+), magnesium (Mg2+), calcium (Ca2+), barium , strontium (Sr2+), and ammonium (NH4+) such as in particular the respective alkaline or alkaline earth salt. Most preferably the respective sodium potassium or calcium salts.
Methane emission by ruminants can easily be measured in individual animals in metabolic chambers by methods known in the art ger et al., 2007 J. Dairy Science; 90: 2755- 2766). Moreover, it can also be assessed at barn level by an emerging technology using laser beam (McGinn et al., 2009, Journal of nmental Quality; 38: 1796-1802). Alternatively , methane produced by a dairy ruminant can also be assessed by measurement of fatty acid es in milk according to Ruminant performance can be assessed by methods well known in the art, and is often il- lustrated by the acetate proprionate ratio (mol ratio).
The present invention also relates to the use of a feed ition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as de- fined by formula (I) in combination with at least one additional active substance which shows similar effects with regard to methane ion in the rumen and which is selected from the group consisting of diallyl disulfide, garlic oil, allyl isothiocyanate, deoxycholic acid, chenodeoxycholic acid and derivatives thereof. r components that could be given together with the compound according to the present invention are for e yeasts, oregano extracts, and essential oils e.g. thymol, 3-methylphenol, vanillin, guaiacol and eugenol.
It is at present contemplated that diallyl disulfide, garlic oil, allyl isothiocyanate deoxycholic acid, chenodeoxycholic acid and derivatives thereof are independently administered in dosage ranges of for example 0.01-500 mg active substance per kg feed (ppm). These compounds are either cially available or can easily be prepared by a skilled person using ses and methods well-known in the prior art.
Ruminating mammals according to the t invention include cattle, goats, sheep, giraffes , American Bison, European bison, yaks, water o, deer, camels, alpacas, llamas, wildebeest, antelope, orn, and nilgai.
For all embodiments of the present invention, domestic cattle, sheep and goat are the more red s. For the present purposes most preferred species are domestic cattle.
The term includes all races of domestic cattle, and all production kinds of cattle, in particular dairy cows and beef cattle.
The present invention also relates to the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the methane tion in nts is reduced by at least 10 % when measured in metabolic chambers and based on the treatment with the at least one organic molecule substituted at any position. Preferably, the methane reduction is at least 15 %, more preferably, at least 20 %, even more preferably, at least %, most preferably, at least 30 %. Alternative methane emission ements may also be used like using a laser beam or for dairy nts, correlating methane production to the VFA profile in milk.
The present invention also s to the use of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the volatile fatty acid ratio of acetate to propionate is reduced by at least 10 % based on the treatment with the at least one organic molecule substituted at any position with at least one nitrooxy group. Preferably, volatile fatty acid ratio is reduced by at least 15 %, more preferably, by at least 20 %.
The present invention also s to the use of a feed composition or feed additive compris- ing at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule tuted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the amount of the organic molecule as defined in formula (I) administered to the ruminant animal is from 1 mg to 10 g per Kg of feed, preferably from 10 mg to 1 g per Kg of feed, more preferably, from 50 mg to 500 mg per Kg of feed, and the amount of the at least one fatty acid ning at least 5 carbon atoms administered to the ruminant animal is from 0.1 to 20 g per Kg of feed, ably from 1 to 15 g per Kg of feed, most preferably from 1 to 10 g per Kg of feed. For the use in animal feed, however, organic molecules substituted at any position with at least one nitrooxy group, or their salts thereof as defined by formula (I) need not be that pure; it may e.g. include other compounds and derivatives.
The present invention further relates to the use of a feed composition or feed ve comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic le tuted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I), wherein the mol ratio of the organic molecule as defined in formula (I) to the at least one fatty acid containing at least 5 carbon atoms in the feed composition or feed additive is comprised between 0.00001 and 1, preferably n 0.0001 and 0.5, more preferably between 0.0025 and 0.1, most preferably in the range of 0.004 to 0.05.
Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01.
Ruminant feed or feed additives may be prepared by methods known per se in the art of feed formulation and processing.
Further aspects of the present ion are ore formulations, i.e. feed additives and animal feed compositions containing compositions as herein above defined.
The present invention ore also relates to a feed composition or a feed additive comprising at least one fatty acid containing at least 5 carbon atoms and at least a compound of formula (I) or a salt thereof. In a preferred ment, the composition is a mineral premix, a vitamin premix including vitamins and minerals or a bolus.
The normal daily dosage of a ition according to the invention provided to an animal by feed intake depends upon the kind of animal and its condition. Normally this dosage should be in the range of from about 1 mg to about 100 g, preferably from about 1 g to about 50 g, more preferably, 5 g to 10g compound per kg of feed.
The ition comprising at least one fatty acid containing at least 5 carbon atoms and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) may be used in combination with conventional ingredi- ents present in an animal feed composition (diet) such as calcium carbonates, electrolytes such as ammonium chloride, proteins such as soya bean meal, wheat, , sunflower meal, corn, meat and bone meal, amino acids, animal fat, vitamins and trace minerals. ular examples of compositions of the invention are the following: - An animal feed additive comprising (a) at least one compound selected from table 1 and (b) at least one fatty acid containing at least 5 carbon atoms, (c) at least one fat-soluble vitamin, (d) at least one soluble vitamin, (e) at least one trace mineral, and/or (f) at least one macro mineral; - An animal feed composition comprising at least one fatty acid containing at least 5 carbon atoms and at least one compound selected from table 1 and a crude protein t of 50 to 800 g/kg feed.
The led premixes are examples of animal feed additives of the invention. A premix designates a preferably uniform e of one or more micro-ingredients with diluents and/or carrier. Premixes are used to facilitate uniform dispersion of micro-ingredients in a larger mix.
Apart from the active ingredients of the invention, the premix of the invention contains at least one fat-soluble vitamin, and/or at least one water soluble vitamin, and/or at least one trace mineral, and/or at least one macro mineral. In other words, the premix of the invention comprises the at least one compound according to the invention together with at least one additional component selected from the group consisting of fat-soluble vitamins, watersoluble vitamins, trace minerals, and macro ls.
Macro minerals may be separately added to the feed. ore, in a particular embodiment, the premix comprises the active ingredients of the invention together with at least one additional component selected from the group consisting of luble vitamins, water-soluble vitamins, and trace-minerals.
The following are non-exclusive lists of es of these components: - Examples of luble vitamins are vitamin A, vitamin D3, vitamin E, and vitamin K, e.g. vitamin K3.
- Examples of water-soluble vitamins are vitamin B12, biotin and choline, vitamin B1, vitamin B2, vitamin B6, niacin, folic acid and panthothenate, e.g. Ca-D-panthothenate.
- Examples of trace minerals are manganese, zinc, iron, copper, iodine, selenium, and cobalt.
- Examples of macro minerals are calcium, phosphorus and sodium.
As regards feed compositions for ruminants such as cows, as well as ingredients thereof, the ruminant diet is usually ed of an easily degradable fraction (named concentrate) and a rich less readily degradable fraction (named hay, forage, or roughage).
Hay is made of dried grass, legume or whole cereals. Grasses include among others timothy, ryegrasses, s. Legumes include among others , lucerne or alfalfa, peas, beans and vetches. Whole s include among others barley, maize (corn), oat, sorghum. Other forage crops include sugarcane, kales, rapes, and cabbages. Also root crops such as turnips, swedes, mangles, fodder beet, and sugar beet (including sugar beet pulp and beet molasses) are used to feed ruminants. Still further crops are tubers such as potatoes, cassava and sweet potato. Silage is an ensiled version of the rich fraction (e.g. from grasses, legumes or whole cereals) whereby material with a high water content is treated with a controlled anaerobic fermentation process (naturally-fermented or additive treated).
Concentrate is largely made up of cereals (such as barley including brewers grain and distillers grain, maize, wheat, sorghum), but also often contain n-rich feed ingredients such as soybean, rapeseed, palm kernel, cotton seed and sunflower.
Cows may also be fed total mixed rations (TMR), where all the dietary components, e.g. , silage and concentrate, are mixed before serving.
As mentioned above a premix is an example of a feed additive which may comprise the active compounds according to the invention. It is understood that the compounds may be administered to the animal in different other forms. For example, the compounds can also be ed in a bolus that would be placed in the rumen and that would release a defined amount of the active compounds uously in well defined dosages over a ic period of time.
The present invention further relates to a method for reducing the production of methane ing from the digestive activities of ruminants and/or for improving ruminant animal performance, comprising orally administering a sufficient amount of a feed composition or feed additive comprising at least one fatty acid containing at least 5 carbon atoms, and at least one organic molecule substituted at any position with at least one nitrooxy group, or a salt thereof as defined by formula (I) with the preferred embodiments described above.
Moreover, the ion further relates to a method as described above, wherein the feed composition or feed additive according to the present invention is administered to the animal in combination with at least one additional active substance selected from the group consisting of diallyl disulfide, garlic oil, allyl isothiocyanate, holic acid, chenodeoxy- cholic acid and derivatives thereof.
The invention also relates to a method as described above, wherein the ruminant animal is selected from the group consisting of: cattle, goats, sheep, giraffes, an Bison, European bison, yaks, water buffalo, deer, camels, alpacas, llamas, wildebeest, antelope, pronghorn, and nilgai, and more ably from the group consisting of: cattle, goats and sheep.
The invention also relates to a method as described above, wherein the amount of the at least one organic molecule as defined in formula (I) administered to the ruminant animal is from about 1 mg to about 10 g per kg feed, preferably from about 10 mg to about 1 g, more preferably from 50 mg to 500 mg compound per kg of feed, and the amount of the at least one fatty acid containing at least 5 carbon atoms administered to the ruminant animal is from 0.1 to 52 g per Kg of feed, preferably from 1 to 15 g per Kg of feed, most preferably from 1 to 10 g per Kg of feed.
The invention also relates to a method as described above, wherein the methane production in nts is reduced by at least 10 % when ed in metabolic chambers and based on the treatment with the at least one c molecule substituted at any position.
Preferably, the methane reduction is at least 15 %, more preferably, at least 20 %, even more preferably, at least 25 %, most preferably, at least 30 %. Alternative e emission ements may also be used like using a laser beam or for dairy ruminants, correlating methane production to the VFA profile in milk.
The invention also relates to a method as described above, n the volatile fatty acid ratio of e to propionate (mol ratio) is reduced by at least 10 % based on the treatment with the at least one c molecule substituted at any position with at least one nitrooxy group. Preferably, le fatty acid ratio is reduced by at least 15 %, more preferably, by at least 20 %.
The invention also relates to a method as described above, wherein the mol ratio of organic molecule as defined in formula (I) to at least one fatty acid containing at least 5 carbon atoms in the feed composition or feed additive is comprised between 0.00001 and 1, preferably between 0.0001 and 0.5, more preferably between 0.0025 and 0.1, most preferably in the range of 0.004 to 0.01. Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01.
In a particular embodiment the present invention relates to the use of a mixture of 3-nitrooxypropanol and lauric acid to reduce the methane formation in ruminants and/ or to decrease the e/ propionate ratio (mol-ratio) in ruminants. In a preferred embodiment, the mole-ratio of 3-nitrooxypropanol to lauric acid in the mixture is ed in the range of 0.00001 to 1, preferably in the range of 0.0001 to 0.5, more preferably in the range of 0.0025 to 0.1, most preferably in the range of 0.004 to 0.01. Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01. In a further preferred embodiment, the e reduction is at least 15 %, more preferably, at least 20 %, even more ably, at least 25 %, most preferably, at least 30 % based on the administration of 3-nitrooxypropanol alone (measured in metabolic rs) and/ or the decrease in the acetate propionate ratio is at least 3%, more preferably at least 4 % based on the administration of 3- nitrooxypropanol alone.
In another embodiment the invention relates to a reduction of the methane formation and/or decrease in the acetate/ propionate ratio atio) in ruminants said method encompassing the step of administering to a ruminant a mixture of ooxypropnaol and lauric acid.
In a preferred embodiment, the mole-ratio of 3-nitrooxypropanol to lauric acid in the mixture is selected in the range of 1 to 1, ably in the range of 0.0001 to 0.5, more ably in the range of 0.0025 to 0.1, most preferably in the range of 0.004 to 0.01. Further preferred ranges encompass 0.003 to 0.05 or 0.004 to 0.01. In a further preferred embodiment , the methane reduction is at least reduction is at least 15 %, more preferably, at least %, even more preferably, at least 25 %, most preferably, at least 30 % based on the ad- ministration of 3-nitrooxypropanol alone (measured in metabolic chambers) and/ or the de- crease in the acetate propionate ratio is at least 3%, more preferably at least 4 % based on the administration of 3-nitrooxypropanol alone.
The present invention is further described by the following es which should not be construed as limiting the scope of the invention.
Examples In vitro test for methane production: A modified version of the "Hohenheim Forage value Test (HFT)" was used for testing the effect of specific compounds on the rumen functions mimicked by this in-vitro system.
Principle: Feed is gadded into a syringe with a composition of rumen liquor and an appropriate e of buffers. The solution is incubated at 39°C. After 8 hours the quantity (and composition) of gas phase produced is measured and put into a formula for conversion.
Reagents: Mass element solution: - 6.2 g potassium dihydrogen phosphate (KH2PO 4) - 0.6 g magnesium sulfate heptahydrate (MgSO4 * 7H2O) - 9 ml concentrated phosphoric acid (1 mol/l) - dissolved in distilled water to 1 l (pH about 1.6) Buffer solution: - 35.0 g sodium hydrogen carbonate (NaHCO3) - 4.0 g ammonium en carbonate HCO3) - dissolved in distilled water to 1 l Trace element solution: - 13.2 g calcium chloride dihydrate (CaCl2 * 2H2O) - 10.0 g ese(II) de tetrahydrate (MnCl2 * 4H2O) - 1.0 g cobalt(II) chloride hexahydrate (CoCl2 * 6H2O) - 8.0 g iron(III) de (FeCl3 * 6H2O) - dissolved in distilled water to 100 ml Sodium salt solution: - 100 mg sodium salt - dissolved in distilled water to 100 ml Reduction solution: - first 3 ml sodium hydroxide (c = 1 , then 427.5 mg sodium sulfide hydrate (Na 2S * H2O) are added to 71.25 ml H2O - solution must be prepared shortly before it is added to the medium solution Procedure: Sample weighing: The feed stuff is sieved to 1 mm - usually TMR (44 % concentrate, 6 % hay, 37 % maize silage and 13 % grass silage) - and weighed exactly into 64 syringes. 4 of these syringes are the substrate controls, which display the gas production t the ef- fect of the tested compounds. 4 other syringes are positive l, in which bromoethane sulfonate has been added to 0.1mM. When needed, 4 es contain a carrier control (if the test compounds need a carrier). The remaining syringes contain the test substances, by groups of 4 es.
Preparation of the medium solution: The components are mixed in a Woulff bottle in following order: - 711 ml water - 0.18 ml trace element solution - 355.5 ml buffer solution - 355.5 ml mass element solution The completed solution is warmed up to 39 °C ed by the on of 1.83 ml sodium salt solution and the addition of reduction solution at 36 °C.
The rumen liquor is added, when the indicator turns colourless.
Extraction of the rumen liquor: 750 ml of rumen liquor are added to approximately 1,400 ml of medium on under continued agitation and ssing.
Filling the syringes, incubation and determining gas volumes and VFA values: The diluted rumen fluid (24 ml) is added to the glass syringe. The syringes are then incubated for 8 hours at 39 °C under gentle agitation. After 8 hours, the volume of gas produced is measured , and the percentage of methane in the gas phase is determined by gas chromatography.
Results The food fermented was artificial TMR (44 % concentrate, 6 % hay, 37 % maize silage and 13 % grass silage). Lauric acid from Sigma Aldrich was used at a concentration of 1000 µM. 3-Nitrooxy-propanol (3-NOP) was used at two different concentrations, 2.5 µM and 5 µM.
The results are presented in the following Table 2 and 3. Clear synergistic effects were obtained for the combination of 3-NOP and Lauric acid in view of the methane reduction as well as on the acetate/propionate ratio, which translates into additional performance benefit for the animal. The results ed below result from the average of three experiments with either Lauric acid, 3-NOP, or a combination of both. Ace. = acetate; Prop. = propionate Table 2: Effect on methane production 3-NOP Lauric acid Total methane Reduction Expected Synergy° Treatment vs. control reduction* [µM] [µM] [mmol over 8h] [%] 1 (Control) - - 0.267 - - - 2 (Ref) 2.5 - 0.244 0.023 - - 3 (Ref) 5 - 0.097 0.17 - - 4 (Ref) - 1000 0.255 0.012 - - (Inv) 2.5 1000 0.125 0.142 0.232 -46 6 (Inv) 5 1000 0.022 0.245 0.085 -74 *Expected ion based on an additive effect vs. control °(Found value - expected value)/ expected value * 100% Table 2: Effect on volatile fatty acids (VFA) profile, i.e. acetate/ propionate ratio ion Expected Synergy° Treatment 3-NOP Lauric acid Ace./ Prop. vs. control reduction* [µM] [µM] [Mole Ratio] [%] 1 (Control) - - 2.26 - - - 2 (Ref) 2.5 - 2.09 0.17 - - 3 (Ref) 5 - 1.63 0.63 - - 4 (Ref) - 1000 1.98 0.28 - - (Inv) 2.5 1000 1.63 0.63 1.81 -9.9 6 (Inv) 5 1000 1.29 0.97 1.35 -4.4 *Expected reduction based on an additive effect vs. control d value - expected value)/ expected value * 100%
Claims (12)
1.Claims
2. Use of a feed composition or feed additive comprising lauric acid and 3-- nitrooxypropanol, for reducing the formation of methane emanating from the ive 5 activities of ruminants, wherein the mol ratio of 3-nitrooxypropanol to lauric acid is comprised between 0.0025 and 0.1 and the amount of 3-nitrooxypropanol administered to the ruminant animal is from 1 mg to 10 g per kg feed, and the amount of lauric acid administered to the ruminant animal is from 0.1 to 20 g per Kg of feed. 10 2. The use according to claim 1, wherein the ruminant animal is selected from the group consisting of: cattle, goats, sheep, giraffes, American Bison, European bison, yaks, water buffalo, deer, , alpacas, llamas, wildebeest, antelope, pronghorn, and 15
3. The use according to claim 1 or 2, n the methane production in ruminants is reduced by at least 10 % when measured in metabolic chambers and based on the treatment with the 3-nitrooxypropanol.
4. The use according to any of claims 1 to 3, wherein the amount of 3-nitrooxypropanol 20 administered to the ruminant animal is from 10 mg to 1 g per Kg of feed, and the amount of lauric acid administered to the ruminant animal is from 1 to 15 g per Kg of feed.
5. The use ing to any of claims 1 to 3, wherein the amount of 3-nitrooxypropanol 25 administered to the ruminant animal is from 50 mg to 500 mg per Kg of feed, and the amount of lauric acid administered to the ruminant animal is from 1 to 10 g per Kg of feed.
6. The use according to any of claims 1 to 5, wherein the mol ratio of ooxypropanol 30 to lauric acid is comprised between 0.004 to 0.01.
7. A ruminant animal feed ition or feed additive comprising lauric acid and 3- nitrooxypropanol, characterized in that the mol ratio of 3-nitrooxypropanol to lauric acid is comprised between 0.0025 and 0.1 and the composition or feed additive is 35 adapted to administer 3-nitrooxypropanol to the ruminant animal in a range of from 1 mg to 10 g per kg feed, and the composition or feed additive is adapted to administer lauric acid to the ruminant animal in a range of from 0.1 to 20 g per Kg of feed.
8. The ruminant animal feed composition or feed additive according to claim 7, wherein 5 the mol ratio of 3-nitrooxypropanol to lauric acid is comprised n, preferably between 0.004 to 0.01.
9. The ruminant animal feed ition or feed additive according to claim 7 or 8, wherein the composition or feed additive is adapted to administer ooxypropanol
10. To the ruminant animal in a range of from 10 mg to 1 g per Kg of feed, preferably from 50 mg to 500 mg per Kg of feed, and the composition or feed additive is adapted to administer lauric acid to the nt animal in a range of from 1 to 15 g per Kg of feed, preferably from 1 to 10 g per Kg of feed. 15 10. A method for ng the production of methane emanating from the digestive activities of ruminants comprising orally administering to the animal a sufficient amount of a feed composition or feed additive sing at least lauric acid, and at least 3- nitrooxypropanol, characterized in that the mol ratio of 3-nitrooxypropanol to lauric acid is comprised between 0.0025 and 0.1 and the amount of 3-nitrooxypropanol admin- 20 istered to the ruminant animal is from 1 mg to 10 g per kg feed, and the amount of lauric acid administered to the ruminant animal is from 0.1 to 20 g per Kg of feed.
11. A method ing to claim 10, wherein the amount of 3-nitrooxypropanol administered to the ruminant animal is from 1 mg to 10 g per Kg of feed, preferably from 10 25 mg to 1 g per Kg of feed, more preferably from 50 mg to 500 mg per Kg of feed, and the amount of lauric acid administered to the ruminant animal is from 0.1 to 20 g per Kg of feed, preferably from 1 to 15 g per Kg of feed, most preferably from 1 to 10 g per Kg of feed. 30
12. A method according to claim 10 or 11, wherein the ruminant animal is selected from the group consisting of: cattle, goats, sheep, giraffes, American Bison, European bison , yaks, water buffalo, deer, camels, alpacas, llamas, wildebeest, antelope, pronghorn , and nilgai.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US201762461485P | 2017-02-21 | 2017-02-21 | |
US62/461,485 | 2017-02-21 | ||
CH2332017 | 2017-02-28 | ||
CH00233/17 | 2017-02-28 | ||
PCT/EP2018/053341 WO2018153702A1 (en) | 2017-02-21 | 2018-02-09 | Use of a feed composition for reducing methane emission in rumi-nants, and/or to improve ruminant performance |
Publications (2)
Publication Number | Publication Date |
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NZ756537A NZ756537A (en) | 2022-03-25 |
NZ756537B2 true NZ756537B2 (en) | 2022-06-28 |
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