WO2018133660A1 - Capsule de collecte de micro-organismes gastro-intestinaux et système de collect. - Google Patents

Capsule de collecte de micro-organismes gastro-intestinaux et système de collect. Download PDF

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Publication number
WO2018133660A1
WO2018133660A1 PCT/CN2018/000024 CN2018000024W WO2018133660A1 WO 2018133660 A1 WO2018133660 A1 WO 2018133660A1 CN 2018000024 W CN2018000024 W CN 2018000024W WO 2018133660 A1 WO2018133660 A1 WO 2018133660A1
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WIPO (PCT)
Prior art keywords
capsule
collection
gastrointestinal
gastrointestinal microbial
sample
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PCT/CN2018/000024
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English (en)
Chinese (zh)
Inventor
王邦茂
王罡
曹海龙
荆洋
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天津医科大学总医院
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Publication of WO2018133660A1 publication Critical patent/WO2018133660A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0038—Devices for taking faeces samples; Faecal examination devices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B2010/0061—Alimentary tract secretions, e.g. biliary, gastric, intestinal, pancreatic secretions

Definitions

  • the present invention relates to the field of other methods or instruments for diagnosis, and in particular to a gastrointestinal microbial collection capsule and collection system.
  • fecal analysis is commonly used in the diagnosis of infectious gastroenteritis, and fecal analysis works well in this application.
  • fecal analysis has a significant impact on analytical results such as microbial composition and abundance due to differences in the collection, storage, and processing of samples during the study or diagnosis of other diseases.
  • An ideal specimen was obtained by a simple and standardized collection method, and there was no damage to the microbial sample during the process.
  • fecal samples are usually collected by patients at home, and the home environment can cause potential impact on the sample or even contamination, thus obstructing the clinical application of microbial sample analysis.
  • the results of the fecal analysis are only representative of the microbial samples in the colon and rectum. It is impossible to obtain the difference in microbial composition and abundance of each segment of the gastrointestinal tract of each subject by fecal analysis.
  • the second method is to use a rectal swabbing, swab the swab (swab) into the anus about 5 cm, and collect a sample for analysis.
  • This method is more acceptable to patients than gastrointestinal mirrors.
  • This method has been widely used for the screening of some rectal microorganisms, but it cannot be widely extended to sample collection in other segments of the gastrointestinal tract.
  • the third method is to collect a small sample of the gastrointestinal epithelium by endoscopic biopsy. Although such methods are highly standardized, their high invasiveness makes them unusable for screening and follow-up. It is particularly worth mentioning that the cleansing enema step prior to the endoscope affects the number and diversity of microorganisms in the gut. As a result, there is still a large doubt about the representativeness of the microbial samples on the collected epidermis. In addition, both the gastroscope and the colonoscope have their distance limitations, and the epidermis specimens of the entire small intestine cannot be collected from either side.
  • the fourth method is to collect intestinal fluid specimens in the gastrointestinal tract using intestinal collection capsules developed based on microelectromechanical technology.
  • the advantage of such a method is that it is minimally invasive.
  • the leading company in this field is the Dutch-based Medimetrics, which developed the Intellicap capsule system including an MEMS-based orally available electronic capsule.
  • the electronic capsule After being orally administered by the patient, the electronic capsule has a built-in micro-detector that can detect the pH and temperature status in the gastrointestinal tract in real time, and can transmit signals to the external controller through its built-in wireless transmitter in real time; at the same time, its built-in wireless receiver It is also possible to receive commands from the external controller for real-time transmission, thereby controlling the operation of a built-in micropump.
  • Its micropump includes an electric motor, a piston and a reservoir.
  • the electric motor can advance the piston forward or backward in a straight line, and the volume of its reservoir is determined by the position of the piston.
  • the liquid storage chamber is sealed by the capsule shell and the piston, and only communicates with the external through a small hole in the capsule shell. When the electric motor pushes the piston backwards, the volume in the liquid storage chamber becomes larger, thereby forming a negative pressure, and a suction force is generated through the small hole to absorb the liquid in the outer gastrointestinal tract of the capsule into the liquid storage chamber.
  • capsules use MEMS technology and require a staff member to continuously monitor the pH and temperature status of the detector to determine the position of the capsule in the gastrointestinal tract to control the operation of the capsule to activate its micropump.
  • the manufacturing cost and the use cost are extremely high, and it is difficult to widely spread and apply in scientific research and clinical practice.
  • the gastrointestinal tract often has solid matter such as food, biological tissue and other components, when the diameter of the pores of such capsules is too small, it will cause the solid matter sucked by the negative pressure to block the pores, and even the sample sucked into the reservoir. The number is small and the representativeness is lost.
  • the diameter of the pores of such capsules is too large, the microbial sample inhaled into the reservoir cavity will exchange and exchange microorganisms in the environment of the whole gastrointestinal region, so that the sample is contaminated. Loss of representation.
  • Another type of electronic capsule technology for gastrointestinal diseases such as gastrointestinal bleeding
  • electronic capsules developed based on such techniques such as Nemiroski, et al. "Swallowable Fluorometric Capsule for Wireless Triage of Gastrointestinal Bleeding," Lab Chip 15,4479 (2015), has the function of collecting and fixing the liquid sample in the gastrointestinal tract in a semi-closed capsule cavity; a micro blood detector based on photoelectric technology is provided in the capsule cavity for instantaneous detection of red blood cell content in the liquid sample The test results are sent to the outside of the body through wireless transmission technology.
  • such capsules do not function to isolate specimens. Specimens are highly contaminated by microorganisms in other gastrointestinal tract environments during passage through the gastrointestinal tract and excreted from the body, and therefore do not apply to gastrointestinal microbes. Sample collection.
  • electronic capsules based on MEMS technology including electronic gastroscope capsules and drug delivery capsules, have a risk of gastrointestinal retention after being taken by the patient.
  • the risk of retention is as high as 15%.
  • Electronic capsules contain toxic substances due to their internal micro-electromechanical components and structures, such as batteries.
  • the gastrointestinal motility promotes the drug and promotes gastrointestinal motility to expel the capsule. If the drug stimulation still does not promote the exclusion of the capsule, the doctor will remove the capsule by gastrointestinal endoscopy or even open surgery according to the position of the capsule.
  • the invention is to solve the problem that the existing gastrointestinal microbial ecological sample collection methods have limitations and cannot be applied to a large area of healthy people, and provides a gastrointestinal microbial collection capsule and an acquisition system.
  • a gastrointestinal microbial collection capsule comprising one or more sets of spontaneous collection and separation devices, the collection and separation device comprising a capsule shell and a capsule shell bottom cover, wherein the capsule shell and the capsule shell bottom cover form a capsule cavity Forming at least one liquid flow channel on the capsule shell;
  • the sample shell is provided with a sample separation device, and the sample separation device comprises a piston on one side of the liquid flow channel between the piston and the capsule shell or the capsule bottom cover a spring and a limiter attached to the piston.
  • the limiting device comprises a soluble body and a linkage extending through the through hole of the soluble body and the capsule shell top and movably connected to the piston.
  • linkage is a screw or a closed traction belt/rope.
  • a bottom of the piston is provided with a piston base, and the limiting device is connected to the piston base.
  • the capsule shell and the capsule bottom cover are in a plug-in connection or a threaded connection.
  • the spring is a coil spring, a tension spring or a compression spring.
  • a hydrophilic fibrous material is provided in the capsule cavity on the side where the liquid flow path is formed.
  • the spring or the linkage is made of metal.
  • the material of the capsule shell, the capsule bottom cover and the piston base is wholly or partially made of a bioabsorbable material.
  • the exterior of the collecting and separating device is coated with a sample collecting and starting component, and the sample collecting and starting component comprises an outer capsule body and an enteric coating.
  • outer capsule body and the capsule bottom cover are plug-in or screwed.
  • sample collection and activation assembly further includes an outer capsule cap disposed on the outer capsule body, the outer capsule body being in a plug-in connection or a threaded connection with the outer capsule cap.
  • dissolution rate of the sample separation device and the sample collection initiation assembly is related to the material, the pH of the gastrointestinal tract, temperature, and gastric motility.
  • a gastrointestinal microbial collection capsule comprises two sets of collecting and separating devices, and two sets of collecting and separating devices share a capsule bottom cover.
  • the collection capsule further includes a detection system including a micro controller and an antenna and a sensor connected to the micro controller.
  • the detection system further includes a magnet.
  • the senor includes an image sensor, a pH sensor, a temperature sensor, a humidity detector, and a pressure sensor.
  • the humidity detector and the pressure sensor pass through the capsule bottom cover and are placed in a capsule cavity forming a side of the liquid flow channel.
  • a gastrointestinal microbial collection system comprising at least one gastrointestinal microbial collection capsule and a capsule collection detection device.
  • the capsule collection detecting device comprises a container and a metal detector.
  • the metal detector is placed in a sealed cavity at the bottom of the container; the metal detector includes a first coil and a second coil, and the first coil and the second coil are respectively connected to the signal transmitter and the signal receiver in the sealed box
  • the signal transmitter and the signal receiver are both connected to the main controller, and the main controller is connected to the buzzer.
  • the present invention achieves the following advantageous effects.
  • the capsule of the invention can automatically complete the collection of gastric juice or intestinal fluid sample and sample isolation after reaching the different specific positions of the gastrointestinal tract in the whole collection process, and is painless and non-invasive, and is easily accepted by the user; Collecting samples extending to the distal end of the small intestine and the segments of the large intestine that are inaccessible to the endoscope; after collecting the gastric or intestinal fluid sample, the liquid flow channel is completely closed and cannot be in contact with the external environment, ensuring that the sample is isolated and not contaminated; The gastric juice or intestinal fluid sample is excreted through the capsule, does not cause intestinal obstruction, and is safe; after the capsule is discharged from the body, it enters the capsule collection and detection device, and the metal detector activates the buzzer after detecting the metal component in the capsule, notifying The user collects the capsule in time; the collected intestinal fluid is used to analyze the composition of the microbial components, and the results can be widely used for basic research and clinical diagnosis and treatment related to gastrointestinal microorganisms, and to establish a
  • the capsule If the capsule is retained in the gastrointestinal tract for a long time, the capsule can be slowly degraded in the gastrointestinal tract and decomposed into several smaller diameter structures, so that it can be excreted through the stenosis in the gastrointestinal tract, so that the capsule user can avoid suffering from the gastroscope or The risk of open surgery.
  • Embodiment 1 is a schematic structural view of the inside of a capsule before collecting a liquid sample in Embodiment 1 of the present invention
  • FIG. 2 is a schematic structural view of the inside of a capsule in a process of collecting a liquid sample in Embodiment 1 of the present invention
  • Figure 3 is a schematic view showing the structure of the inside of the capsule after the liquid sample is collected in the first embodiment of the present invention
  • Figure 5 is a schematic view showing the structure of the capsule in the first embodiment of the present invention after long-term retention in the gastrointestinal tract;
  • Figure 6 is a schematic view showing the structure of the inside of the capsule before collecting the liquid sample in Embodiment 2 of the present invention
  • Figure 7 is a schematic view showing the structure of the inside of the capsule in the process of collecting a liquid sample in Embodiment 2 of the present invention.
  • Figure 8 is a schematic view showing the structure of the inside of the capsule after the liquid sample is collected in the second embodiment of the present invention.
  • Figure 9 is a schematic view showing the structure of the inside of the capsule when the sample is disassembled in the second embodiment of the present invention.
  • Figure 10 is a schematic view showing the structure of the inside of the capsule before collecting the liquid sample in Embodiment 3 of the present invention.
  • Figure 11 is a schematic view showing the internal structure of the capsule during the process of collecting intestinal juice by the first collecting and separating device in Embodiment 3 of the present invention.
  • Figure 12 is a schematic view showing the internal structure of the capsule after the collection of intestinal fluid by the first collecting and separating device in the third embodiment of the present invention.
  • Figure 13 is a schematic view showing the internal structure of the capsule during the process of collecting intestinal juice by the second collecting and separating device in Embodiment 3 of the present invention.
  • Figure 14 is a schematic view showing the internal structure of the capsule after the collection of intestinal fluid by the second collecting and separating device in the third embodiment of the present invention.
  • Figure 15 is a schematic view showing the structure of the inside of the capsule before collecting the liquid sample in Embodiment 4 of the present invention.
  • Figure 16 is a schematic view showing the structure of the inside of the capsule in the process of collecting a liquid sample in Embodiment 4 of the present invention.
  • Figure 17 is a schematic view showing the structure of the inside of the capsule after the liquid sample is collected in the fourth embodiment of the present invention.
  • Figure 18 is a schematic view showing the structure of the capsule collecting and detecting device of the present invention.
  • Figure 19 is a schematic structural view of a metal detector of the present invention.
  • Figure 20 is a block diagram showing the system structure of the metal detector of the present invention.
  • outer capsule body 1. outer capsule body; 2. outer capsule cap;
  • Figures 1-5 depict the first structure of a gastrointestinal microbial collection capsule.
  • the outer capsule body 1 and the outer capsule cap 2 constitute the outer casing of the entire outermost collection capsule, and the outer capsule body 1 and the outer capsule cap 2 are first locked by the locking structure 3.
  • the outer capsule body 1 and the outer capsule cap 2 after the closure are coated with one or more layers of the enteric coating 4 to achieve a targeted dissolution function.
  • the outer capsule body 1 and the outer capsule cap 2 were made of AAA DBcaps (inner diameter 11.39 mm, capsule length 16.31 mm, capsule cap length 14.84 mm) produced by Suzhou Capsule (Capsugel).
  • the enteric coating 4 is a composite jejunal targeting enteric material based on Eudragit L100-55 produced by Evonik.
  • the preparation method is as follows:
  • the closed outer capsule cap 2 was immersed in the above-mentioned coating reagent for 15 seconds, taken out and baked in a baking oven at 50 degrees Celsius for 20 minutes. After the coating-bake step was repeated 10 times, the outer capsule 1 was coated and baked 10 times in the same manner.
  • the inside of the sample collection and starting assembly 16 is a spontaneous collecting and separating device.
  • the bottom of the main body of the capsule shell 6 is screwed to the capsule bottom cover 5 by internal and external threads, so that the bottom of the capsule shell 6 is completely sealed.
  • the interior of the capsule shell 6 is a capsule chamber of hollow structure.
  • the side wall of the capsule shell 6 has four apertures penetrating the capsule shell wall, which is a preferred implementation of the liquid flow channel 7.
  • the capsule shell bottom cover 5 and the capsule shell 6 are simple in design, and are manufactured by a precision machine tool PEEK material in a prototype that has been produced on a small scale. In the mass production stage, the capsule shell bottom cover 5 and the capsule shell 6 can also be produced by a compression molding method.
  • the piston 8 is located on the upper side of the liquid flow passage 7 in the capsule chamber, further separating the capsule chamber into a first sub-chamber 9 and a second sub-chamber 10, and isolating the liquid flow between the two sub-chambers.
  • the piston 8 uses a piston element in a Luer-Lok 3 mL needle tube manufactured by BD Corporation.
  • the soluble body 11 is a disc-shaped sugar piece
  • the spring 12 is a compression spring in a high energy storage state
  • the linkage 13 is a flat head screw; after the piston 8 is inserted into the piston base 14 and fixed, the linkage 13 is sequentially worn.
  • the piston base 14 is screwed, and the structural bearing pressure of the soluble body 11 and the energy storage of the compression spring are fixed.
  • the piston 8 is in an initial position on the upper side of the liquid flow path 7 in the capsule chamber.
  • the piston 8 is inserted into the piston base 14, and then inserted into the capsule cavity from the bottom of the capsule shell 6 with the compression spring; then the piston 8 is pushed upward from the bottom of the capsule shell 6, and the piston 8 is placed against the elastic force of the compression spring.
  • the gastrointestinal microbial collection capsule is first contacted with the liquid in the gastrointestinal tract after the user enters the gastrointestinal tract orally; in the prototype that has been produced, the sample collection activation component 16 is a duodenum target. To the coating material Eudragit L100-55. Therefore, the sample collection activation assembly 16 should remain structurally intact and insoluble in the stomach.
  • the sample collection activation assembly 16 dissolves.
  • the intestinal fluid surrounding the capsule passes through the liquid flow channel 7 penetrating the capsule shell wall, and flows into the second sub-chamber 10 by its own gravity, intestinal peristalsis, and suction of the hydrophilic fiber 15, and is fixed in the hydrophilicity by adsorption.
  • the surface of the fiber 15 is.
  • the soluble body 11 in the sample separation device is melted, and its structural disintegration causes the compression spring to exert its energy storage, and the force released when the high energy storage form changes to the low energy storage form moves the position of the piston 8 to Between the liquid flow channel 7 and the capsule bottom cover 5, a certain amount of liquid sample 17, i.e., intestinal fluid, is enclosed in the second sub-chamber 10.
  • the capsule bottom cover 5 is unscrewed to complete the disassembly.
  • the hydrophilic fibers 15 in the capsule shell 6 and the intestinal fluid sample attached thereto can be easily taken out for biochemical detection in the laboratory, such as 16s rRNA detection.
  • the material of the capsule shell 6, the capsule shell bottom cover 5, and the piston base 14 can be slowly degraded in the gastrointestinal tract and decomposed into several diameters.
  • the small structure facilitates passage through the stenosis in the gastrointestinal tract and is thereby excreted, so that the capsule user avoids the risk of endoscopic or open surgery.
  • the material of the capsule shell 6, the capsule shell bottom cover 5, and the piston base 14 may be a bioabsorbable material such as bioabsorbable polydioxanone.
  • Bioabsorbable poly(dioxa) is a new synthetic material, and because of its robust structure, it has been used as a degradable scaffold for the treatment of esophageal stricture. It degrades slowly after several weeks of implantation (Dhar , et al.”Biodegradable stent or balloon dilatation for benign
  • FIG. 6-9 Depicted in Figures 6-9 is a second configuration of a gastrointestinal microbial collection capsule.
  • the spring 12 is located in the second sub-chamber 10 and is in a stretched state.
  • the piston 8 is moved between the liquid flow path 7 and the capsule bottom cover 5 to close a certain amount of the liquid sample 17.
  • FIG. 10-14 Depicted in Figures 10-14 is a third configuration of a gastrointestinal microbial collection capsule.
  • one collection capsule of the embodiment includes two sets of spontaneous collection and separation devices, and the soluble body 11 of the two collection and separation devices is made of different materials, so that the dissolution time is different, so Digestion samples can be taken separately at different locations in the gastrointestinal tract.
  • the soluble body 11 in the sample separation device can be coated with an enteric coating material (such as Eudragit L100) for a common candy piece, and the sample collection starter assembly 16 can be applied to another enteric coating material for standard gelatin capsules.
  • enteric coating material such as Eudragit L100
  • the sample collection starter assembly 16 can be applied to another enteric coating material for standard gelatin capsules.
  • S100 is suitable for decomposition in the colon; the gastrointestinal microbial collection capsules achieved by this combination are kept intact in the stomach and duodenum after oral administration by the user.
  • the liquid flow channel 7 remains closed, and the gastrointestinal fluid of this segment cannot flow into the second sub-chamber 10 via the liquid flow channel 7; in a later jejunum, the sample collection starter assembly 16 dissolves rapidly, causing the liquid flow channel 7 to open, the jejunum The liquid enters the second sub-chamber 10 via the liquid flow channel 7; in a later colon, the sample separation device dissolves rapidly, thereby causing the spring 12 to release its potential energy, pushing the piston 8 to move and The liquid flow path 7 is closed.
  • the intestinal fluid sample in the second sub-chamber 10 is isolated from the outside world, and thus its components, such as, but not limited to, microorganisms and compound components, represent intestinal fluid components from the jejunum to the colon segment.
  • the soluble body 11 in the sample separation device can be a common candy piece, and the sample collection and activation assembly 16 can be removed from the design, and the liquid flow channel 7 of the gastrointestinal microbial collection capsule realized by the combination is initially In the open state, the gastric juice is immediately absorbed into the stomach by the user; the sample separation device rapidly dissolves in the stomach, thereby causing the spring 12 to release its potential energy, pushing the piston 8 to move and closing the liquid flow channel 7 .
  • the gastric juice sample in the second sub-chamber 10 is isolated from the outside, so its composition represents the gastric juice component.
  • the capsule is further provided with a detection system.
  • the detection system has a microcontroller 18 as a main control unit, and externally connects various sensors, an antenna 19 and a magnet 30.
  • the sensor is one or more miniature sensors including, but not limited to, humidity, pressure, pH, temperature, and image sensors.
  • the microsensor simultaneously detects the physiological condition of the gastrointestinal tract section of the capsule and the state of the gastrointestinal fluid collection and separation device, and inputs a detection signal to the microcontroller 18 built into the capsule.
  • the microcontroller 18 transmits the collected signals to the outside of the body through a miniature antenna built into the capsule.
  • the capsule can control the magnet 30 based on the signal feedback of the sensor by an external magnetic control platform to control the fixation and movement of the capsule in the gastrointestinal tract.
  • Figure 18 is a schematic view showing the structure of a capsule collection detecting device.
  • the structure of the capsule collection detecting device is divided into two parts: a barrel container 25 at the top and a sealing chamber 26 at the bottom, and a sealed metal chamber is built in the sealing chamber 26.
  • the capsule collection and detection device can be placed in a sitting toilet as a whole, so that the user can use the defecation within a few days after the oral gastrointestinal fluid collection capsule. Because each user's gastrointestinal motility is different, the time for capsules to be excreted through the gastrointestinal tract is different. At the same time, capsules are less likely to be visually observed by the user after being excreted with the feces.
  • the metal detector in the capsule collection detecting device can automatically detect whether the capsule is excreted after each defecation: when the capsule stays in the body, it is far from the metal detector, and the signal generated by the metal detector changes little.
  • the buzzer will not be triggered to start; when the capsule is discharged from the anus and then falls into the container 25, close to the metal detector, the signal generated by the metal detector changes significantly; the signal change activates the buzzer, notifying the user that the capsule has been discharged in vitro.
  • the sealed case 27 has built-in electronic components such as a metal detector and a buzzer; both ends of the first coil 28 and the second coil 29 are also connected to the sealed case 27.
  • the sealing chamber 26 is a water-tight structure, and the capsule collecting and detecting device is placed in the sitting toilet, and the electronic components inside can also operate normally.
  • Figure 20 is a block diagram illustration showing the implementation principle of the capsule collection detecting device of the present invention.
  • the main controller control signal transmitter generates a current through the first coil 28 to generate electromagnetic induction to generate a first magnetic field; the first magnetic field passes through the compartment of the container 25 and the sealed chamber 26 via electromagnetic induction, in the compartment A vortex is created in the metal part in the upper capsule.
  • the eddy current again generates a second magnetic field via electromagnetic induction; the second magnetic field creates another eddy current within the second coil 29.
  • the signal receiver receives this eddy current and transmits the signal to the main controller.
  • the intestinal fluid collection capsule stays in the body, it is far from the metal detector, and the flow of the eddy current in the capsule is small, and further, the second magnetic field generated by the capsule and the second magnetic field generated by the second magnetic field are smaller. .
  • the gastrointestinal fluid collection capsule is discharged outside the body, staying in the container 25, closer to the metal detector, the eddy current flow in the capsule is larger, and further, the second magnetic field generated by the second magnetic field and the second magnetic field are in the second coil 29. The resulting current flow is large.
  • the main controller starts the buzzer alarm.
  • the capsule collection detecting device described in the present invention can be applied not only to the detection of the gastrointestinal fluid collection capsule described in the present invention, but also to the collection and detection of any capsule discharge containing metal components because of its basic electromagnetic induction principle.
  • the bottom of the container 25 of the capsule collection and detection device described in the present invention is a sealed structure, and the discharged feces and capsules are not in contact with the surrounding environment, and the intestinal flora carried by the capsule is carried out. It will not be polluted by the micro-ecological environment in the surrounding environment, so that the sample of intestinal flora collected by the capsules of the present invention is more accurate and representative, and can satisfy the clinical and scientific research needs, and has broad application prospects. .

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne une capsule de collecte de micro-organismes gastro-intestinaux et un système de collecte. La capsule de collecte de micro-organismes gastro-intestinaux comprend un ou plusieurs appareils autonomes de collecte et de séparation d'échantillons. L'appareil de collecte et de séparation comprend une coque de capsule (6) et un capuchon inférieur de coque de capsule (5), au moins un canal d'écoulement de fluide (7) étant formé sur la coque de capsule (6). Un appareil de séparation d'échantillon est disposé dans la coque de capsule (6), et l'appareil de séparation d'échantillon comprend un piston (8) situé sur un côté du canal d'écoulement de fluide (7), un ressort (12) situé entre le piston (8) et la coque de capsule (6) ou le capuchon inférieur de coque de capsule (5), et un appareil de limitation relié au piston (8). Le système de collecte comprend au moins une capsule de collecte de micro-organismes gastro-intestinaux et un appareil de collecte et de détection de capsules. La capsule de collecte de micro-organismes gastro-intestinaux peut collecter des échantillons jusqu'à l'intestin grêle distal et les sections de côlon proximales; des échantillons de fluide gastrique ou de fluide intestinal sont automatiquement collectés après que la capsule a atteint différentes positions dans le tractus gastro-intestinal; après la collecte d'échantillons de fluide gastrique ou de fluide intestinal, le canal d'écoulement de fluide (7) est complètement fermé, garantissant que des échantillons de micro-organismes ne sont pas contaminés; et après que la capsule est excrétée, l'appareil de collecte et de détection de capsule peut notifier à un utilisateur de recueillir la capsule.
PCT/CN2018/000024 2017-01-23 2018-01-22 Capsule de collecte de micro-organismes gastro-intestinaux et système de collect. WO2018133660A1 (fr)

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CN201710049217.5 2017-01-23
CN201710049217.5A CN106725634B (zh) 2017-01-23 2017-01-23 肠道微生物采集胶囊

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CN113440172A (zh) * 2021-07-27 2021-09-28 新疆维吾尔自治区人民医院 一种基于互联网的消化道采样胶囊
WO2023146175A1 (fr) * 2022-01-28 2023-08-03 순천향대학교 산학협력단 Dispositif de collecte de microbiome
CN116763359A (zh) * 2022-01-12 2023-09-19 徐昆 一种基于互联网模式的精准生殖道微生态检测系统及其应用
EP4282340A1 (fr) * 2022-05-25 2023-11-29 Stichting IMEC Nederland Dispositif ingérable pour matériau d'échantillonnage et son procédé d'utilisation
KR102669000B1 (ko) * 2022-01-28 2024-05-27 순천향대학교 산학협력단 마이크로바이옴 채취 장치

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