WO2018082236A1 - Cultivation method for multiple-biophase microbial zoogloea used for waste water treatment - Google Patents

Abstract

Provided is a cultivation method for multiple-biophase microbial zoogloea used for waste water treatment, comprising the following steps: establishing an environment for cultivating multiple-biophase microbial zoogloea; screening the multiple-biophase microbial zoogloea; selecting an implantation carrier for the multiple-biophase microbial zoogloea; and domesticating strains.

Description

Method for cultivating multi-biomicrobial micelles for wastewater treatment Technical field

The invention belongs to the technical field of ecological environmental protection, and particularly relates to a method for cultivating a multi-biomicrobial micelle for wastewater treatment.

Background technique

The micelles in the prior art, such as the micelle or biofilm method present in the activated sludge, are formed by the growth of the microbial sludge which is metabolically active in the biological treatment system by the bacterial gums which are fixed and grown on the filler. The bacterial cell inoculation environment is simple, no one is a screening step, the implantation carrier is single and the domestication controllability is poor, and the formed biological phase is often a single aerobic phase or anaerobic equivalent.

The core of the conventional biological filter bed treatment is the microbial implantation filler. The fillers in the prior art are generally classified into three types according to the properties: inorganic filler, organic polymer filler and composite filler. Traditional fillers have the following disadvantages:

1. Traditional fillers have high production cost and low performance;

2. The traditional filler has a low specific surface area and poor adsorption capacity;

3. The traditional filler pollutants have poor degradation ability, the biological phase is unstable, and the microbial species are single.

The above-mentioned fillers are inefficient in treating high-concentration organic wastewater, such as high concentration of COD, ammonia nitrogen and the like in livestock and poultry breeding wastewater. Therefore, there is an urgent need to develop a multi-biomicrobial micelle packing suitable for high-concentration organic wastewater treatment and a culture method thereof to meet increasingly stringent emission requirements.

Summary of the invention

In view of the above deficiencies, the technical problem to be solved by the present invention is to provide a suitable for high concentration. A method for culturing a multi-biological microbial cellulite filler for organic wastewater treatment.

To achieve the above object, the present invention is achieved by the following technical solutions:

A method for cultivating a multi-biomicrobial micelle for wastewater treatment, the method comprising the steps of:

(1) Establish multi-biological microbial bacteria micelle culture environment: select the culture field, carry out oxygen concentration gradient transformation, realize oxygen gradient change, artificially create aerobic, facultative and anaerobic culture environment, and construct a fully enclosed anatomical Oxygen culture field or semi-closed aerobic culture field, the culture environment should meet regional requirements, temperature requirements and organic matter content requirements;

(2) Screening multi-microbial microbial cell micelles: In the first step, the multi-biomicrobial micelles of different years are selected according to the concentration of the wastewater to be treated to screen the mother body; the second step is to obtain aerobic-bio-phase gelatin. Group, facultative-bio-biomass micelles, anaerobic-bio-phase bacteria micelles, organic matter screening materials with different longitudinal depths of landfills are selected respectively; third step, according to multi-biomicrobial microbial gelatin filter bed The bed structure screens different particle size fillers, and the bed structure comprises one or more of an aerobic filter layer, a facultative filter layer, and an aerobic filter layer;

(3) selecting a multi-biological microbial cellulite micelle carrier, and grading the multi-biological microbial cellulite group that meets the aging time requirement of the parent material in step (2) according to the concentration of the wastewater contaminant;

(4) Domestication of strains by multi-biomicrobial micelle filter bed: direct culture with waste water to be treated, alternately by water distribution and drying, and the ratio of dry to wet is (4-8): 1, preferably ( 5-7): 1.

The culture field is selected from a closed or semi-closed pothole, and the organic matter of a certain component is filled in the pothole, and the household garbage is preferably used for cultivation.

The cultivation field selects a domestic waste landfill.

When constructing a fully enclosed anaerobic culture field, the oxygen concentration gradient modification method is: collecting the buried gas channel to prevent combustion or explosion; dividing the air concentration gradient according to the single tube radiation area of 10-20 m ² and the longitudinal depth of 1 to 10 m. The vertical perforated natural ventilation pipe is arranged at different depths; the natural ventilation pipe has a pipe diameter of 100-500 mm, and a vent hole having a diameter of 1-5 cm is opened around the natural ventilation pipe.

When constructing a semi-closed aerobic culture field, the oxygen concentration gradient modification method is: using the design of the leakage flow of the leachate drainage tube and the internal and external temperature difference of the landfill, so that the outside air of the heap naturally enters, and the leachate drainage tube There is a certain aerobic area around the vertical ventilation duct, and the place where the air is not diffused is in an anaerobic state, and different oxygen concentration gradients are directly realized.

The regional requirements are specifically selected for the middle and lower reaches of the Yangtze River Three Gorges with abundant annual precipitation; the temperature requirement is: -10 °C ~ 40 °C; the organic matter content requirement is: organic matter content is 20-50 Within the range of %.

According to the pollutant concentration of the wastewater to be treated, the method for selecting the parent strain of the biological bacteria micelles of different years is as follows:

a. COD concentration is 500-1000mg/l, ammonia nitrogen concentration is 200-500mg/l, and the parent strain of biological bacteria micelles is from the culture field required by step (1) 2 to 5 years after the closure; preferably 3 to 4 years, most Preferably 4 years;

b. COD concentration is 1000-2000mg/l, ammonia nitrogen concentration is 500-1000mg/l, and the parent strain of the biological bacteria micelle is from the culture field required to meet the step (1) 6 to 9 years after the closure; preferably 7 to 8 years, most Excellent 8 years;

c. COD concentration limit 2000mg/l or more, ammonia nitrogen concentration limit 1000mg/l concentration limit, biological bacteria micelle screening mother from 10 to 15 years after closure of the field to meet the requirements of step (1) of the culture field; preferably 11 - 13 years, most preferably 13 years.

The method for selecting organic matter screening materials with different longitudinal depths in the culture field is as follows:

a. aerobic-biological phase micelles: selecting a longitudinal depth of 1 to 4 meters of organic matter screening material; preferably 2-4 meters, most preferably 4 meters;

b. facultative-bio-phase bacteria micelle: select 4~6 meters longitudinal depth organic matter screening material; preferably 5-6 meters, most preferably 6 meters;

c. Anaerobic-biological phase micelles: select 6~10 meters longitudinal depth organic matter sieving Preferably, it is 7 to 10 meters, and most preferably 10 meters.

Preferably, the particle size screening of the selected organic material-based screening materials of different longitudinal depths is performed in the following manner to construct a multi-bio-microbial micelle filter bed: the aerobic filter layer is obtained from the aerobic-bio-phase microbial micelles A coarse filter material having a particle diameter of 4 to 6 cm, preferably 5 to 6 cm, and most preferably 5 cm is selected; and the facultative filter layer has a particle size of 2 to 4 cm, preferably 3 to 4 cm, from the obtained facultative-bio-organism micelle. Preferably, a 4 cm medium particle filter; an anaerobic filter layer is selected from the obtained anaerobic-biofilm micelles having a particle size of 0.5 to 2 cm, preferably 1 to 2 cm, and most preferably 2 cm.

The sorting method for organic matter screening materials is:

1 large block of garbage screening: use the crawler type excavator to determine the excavation depth according to the layer height of different multi-filler requirements, and carry out preliminary screening through the drum screen with a hole diameter of 8cm, which will block large inorganic construction garbage and plastic bag debris. Excluded, the material after screening is ready to enter the next link;

2 Non-degradable plastic screening: According to the density difference between plastic and organic materials, the broken plastic is screened by the air-selection system, and the material after screening enters the next step;

3 Ferromagnetic screening: According to the physical properties of the material, the ferromagnetic material is separated by magnetic separation, and the material after screening enters the next step;

4 Particle size selection: According to the requirements of different wastewater treatment, and the particle size requirements of different bed structures, particle size screening.

The implantation carrier selects one or more of power plant ash, gravel, gravel, quartz sand, sludge ceramsite, calcium carbonate aggregate, and PVC filler ball.

The gradation of multi-biomicrobial micelles and implantation carriers is:

a. COD concentration of 500 ~ 1000mg / l, ammonia nitrogen concentration of 200 ~ 500mg / l, the screening of the requirements of the maternal material aging time requirements of multi-biomicrobial micelles and implantation carrier volume ratio (1 ~ 5): 1;

b. COD concentration is 1000-2000mg/l, ammonia nitrogen concentration is 500-1000mg/l, and the volume ratio of multi-biomicrobial micelles and implantation carrier that meets the requirements of aging time of parent material is (5~10): 1;

c. COD concentration limit 2000mg / above l, ammonia nitrogen concentration limit 1000mg / l concentration limit, the screening of the multi-biomicrobial micelles and the implantation carrier volume ratio to meet the aging time of the parent material is (10 ~ 15) ):1.

The process of acclimating the strains by using the multi-biomicrobial micelle filter bed includes: the first stage: mixing and diluting the wastewater, the influent amount is 10-20% of the design scale, and the operation mode is continuous smoldering for 12-24 hours, no Drainage; the dilution ratio of wastewater and tap water in the mixed dilution wastewater is gradually increased to 1:1; each dilution ratio is one cycle, and the mixed wastewater of the next dilution ratio is vented before entering the water; the second stage: raw water to be treated The amount of water inflow is gradually increased from 40% of the design scale to 100%, and water is fed into 4-6 cycles. Each time the water is 10% to 15%, it is dried for 3-5 hours, and the air is ventilated for 2 to 4 hours.

The beneficial effects of the invention are: "multi-biomicrobial microbial micelle" filler is organic matter under different circumstances, and is alternately grown under aerobic, anaerobic and anaerobic conditions for a long time, after many years of physical, chemical and biodegradation. The role of the final formation of a class of soil loose materials with relatively stable properties and components. The microbial flora is rich in microorganisms, and the variety is numerous. The appearance has the characteristics of multi-phase porosity and large surface area, and has the advantages of high nutrient content. Through the analysis of physical and chemical properties of bacteria micelles, it shows that it has excellent purification performance of pollutants which is difficult to form under natural conditions, and is an excellent biological treatment medium for wastewater.

Through the inoculation environment of multi-biomicrobial micelles, the screening of multi-biomicrobial micelles, the selection of bacterial micelles, and the combination of biological strains to achieve the cultivation of multi-biomicrobial micelles. To meet the needs of wastewater treatment for microbial species. The multi-biomicrobial micelles cultured by the method have strong adaptability and stable operation, and realize high concentration organic waste. Efficient, low-cost processing of water.

DRAWINGS

Figure 1 is a schematic view showing the structure of an anaerobic culture field of a multi-biomicrobial micelle;

Fig. 2 is a schematic view showing the structure of aerobic culture field of a multi-biomicrobial micelle.

detailed description

Based on the principle of waste treatment and resource utilization, the present invention preferentially selects the corresponding landfill for culture condition modification, and meets the requirements of temperature, oxygen concentration and organic nutrients for multi-biomicrobial micelle culture; The culture cycle is also the key to selecting multi-biomicrobial micelle packing. After aerobic, facultative and anaerobic oxygen concentration gradient changes, a kind of soil-type microbial community formed by long-term acclimation of high-concentration organic solution has strong anti-shock load capacity, rough living conditions and strong degradation ability.

According to the principle of homologous nitrification and denitrification and anaerobic ammonium oxidation, the multi-bio-phase microbial micelles are screened and cultured, and the large cation exchange capacity and microbial affinity are used to form multi-biological bacteria. Survival of the micro-ecological environment, in order to achieve high-efficiency removal of high concentrations of organic wastewater, especially high concentrations of COD, ammonia and other organic pollutants in livestock and poultry breeding wastewater.

Multi-biological microbial micelles are quite different from conventional wastewater treatment microbial inoculation methods. Conventional microbial inoculation is generally carried out by means of microbial membranes, which are processes in which metabolically active microbial sludge is fixedly grown on a filler in a biological treatment system. The multi-biological microbial micelles are directly mixed with the carrier material, and the microorganisms are cultured by inoculation with the organic matter solution as a nutrient solution to form aerobic microorganisms, nitrifying bacteria, denitrifying bacteria, and anammox bacteria. a class of bacterial micelles.

(1) Establishing a multi-biological microbial cellophore culture environment: selecting a culture field, performing oxygen concentration gradient transformation, realizing oxygen gradient changes, artificially creating aerobic, facultative and anaerobic culture rings To construct a fully enclosed anaerobic culture field or a semi-closed aerobic culture field, the culture environment should meet regional requirements, temperature requirements and organic matter content requirements.

The microbial flora of wastewater treatment includes many types, and can be classified into aerobic microorganisms, facultative anaerobic microorganisms, and obligate anaerobic microorganisms according to their oxygen demand. The multi-biomicrobial micelles include the above three different forms of microbial flora. Due to the different concentrations of oxygen required, it is necessary to cultivate microbial spatial conditions to achieve multiple concentration gradients. The invention selects a fully enclosed anaerobic culture field and a semi-closed aerobic culture field. By changing the oxygen concentration in each area of the culture space, the oxygen gradient is changed, and an aerobic, facultative and anaerobic culture environment is artificially created. Pesticides Environment The present invention preferentially selects a domestic waste landfill and performs transformation according to the above-mentioned culture environment requirements.

Construction of anaerobic culture field: Domestic garbage is layered and naturally stacked on the above-mentioned anti-seepage layer, covered layer by layer, and finally closed. The characteristic of this type of culture field is that before the layered soil seal is not carried out in the early stage, the system environment is in aerobic and facultative state due to the entry of fresh garbage. Due to the presence of organic matter, some aerobic microorganisms are decomposed to decompose the organic matter. A certain amount of aerobic microbial groups. However, as the landfill height increases, the bottom space gradually transitions to an anaerobic state, and some microorganisms are converted into anaerobic microorganisms. After the layered soil seal, the entire system space forms a closed anaerobic space, and the original residual oxygen will disappear, so that the microbial demand for oxygen concentration cannot be achieved. For this reason, if anaerobic is selected in the present invention, At the culture site, the oxygen concentration gradient will be modified in the pre-cultivation field. The transformation method is as follows: 1 Landfill gas drainage is collected to prevent burning or explosion. 2 Set the air transmission pipeline, according to the single-tube radiation area of 10 ~ 20m ² , the longitudinal depth of 1 ~ 10m divided the air concentration gradient to achieve the concentration gradient in the three-dimensional space. If the area of the plant is 10000m ² , it is necessary to arrange 500-1000 longitudinal air pipes in the plane space. The depth of the field selection is 15m. The air pipes should be arranged at different depths of 1~10m to ensure the concentration of oxygen from above and below. Gradient changes. 3 The longitudinal tracheal depth needs to be staggered and the depth is different. It can simulate the change or alternate conversion of microbial species under different oxygen conditions to ensure that the microbe maintains certain biological activity under different oxygen conditions.

Aerobic culture field construction: In order to ensure large-scale replication of multi-biomicrobial micelle packing, the cultivation of multi-biomicrobial micelles in aerobic culture fields is also the focus of the present invention. The aerobic design principle is to use no power to supply oxygen, but to use the design of the leachate drainage tube to use the flow of the leaking internal and external temperature difference, so that the outside air of the reactor naturally enters, in the leachate collection pipe and the vertical ventilation pipe. There is a certain aerobic area around, where the garbage is decomposed by aerobic and the place where the air is not diffused is in an anaerobic state. Compared with the above anaerobic culture field, the quasi-aerobic culture field accelerates the discharge of leachate, inhibits the generation of gases such as methane and hydrogen sulfide, accelerates the stabilization of organic matter, and reduces the concentration of pollutants in the leachate. In this way, the spatial oxygen concentration gradient in the plant area can be directly realized, and the common survival of aerobic, facultative, and anaerobic microorganisms can be realized, and the screening of multi-biomicrobial micelles can be facilitated.

The cultivating site used in the invention utilizes the original site and facilities as much as possible. Since the microorganisms select specificity for the organic matter, the corresponding site should be selected according to different situations, and the present invention preferentially selects the landfill in the corresponding region. Due to the large differences in production and living habits of people in different regions, the garbage components in different regions are also very different. For example, in the northern region, due to the cold weather, coal stoves will generate a large amount of coal ash slag, so the most waste components are inorganic materials such as cinder; in the south, the climate is warm, the diet is rich, and the kitchen waste is more. Most of them are organic wastes such as kitchens and are rich in nutrients. Compared with the southern landfill, it is more suitable for the cultivation of multi-biomicrobial micelles in the present invention. The location of the selected landfill site in the present invention is in the middle and lower reaches of the Yangtze River. This area can not only meet the requirements of the multi-biomicrobial micelles on the organic matter in the garbage, but also meet the temperature change requirements.

1 Regional selection: the strip-shaped plain along the middle and lower reaches of the Yangtze River Three Gorges. North of Huaiyang Mountain, South of the Jiangnan hills. The terrain is low and the ground level is mostly below 50 meters. The middle reaches of the plain include the Jianghan Plain in Hubei, the Dongting Lake Plain in Hunan (collectively known as the Two Lakes Plain), and the Poyang Lake Plain in Jiangxi. The downstream plains include the Yangtze River Plain and the Chaohu Plain in Anhui Province and the Yangtze River Delta between Jiangsu, Zhejiang and Shanghai. The annual precipitation in this area is relatively abundant, which can realize the intermittent impact of landfill leachate on the microbial flora, which is conducive to the cultivation of microbial resistance to load shock.

2 Temperature selection: -10～40°C, the temperature range for maintaining the normal biological activity of microorganisms is about 15～35°C. Through long-term biological domestication, the temperature tolerance is gradually strengthened, but the limit temperature limit cannot be exceeded. Otherwise, Microbes will die or become completely inactive. It is usually preferably 0 to 35 ° C, and most preferably 15 to 35 ° C.

3 Organic matter content: In order to maintain the metabolism of the microorganism's own life, organic matter is indispensable as a nutrient. The present invention requires an organic matter content of between 20 and 50%, preferably 20 to 40%, and most preferably 20 to 30%. Too much will bring secondary organic pollution, increase the concentration of COD and ammonia nitrogen in the bulk filler leachate, too little, which is not conducive to the cultivation of microorganisms.

(2) Screening multi-microbial microbial flora:

(1) selecting the multi-biomicrobial micelles of different years to screen the matrix according to the concentration of the wastewater to be treated;

1COD concentration limit 500～1000mg/l, ammonia nitrogen concentration limit 200～500mg/l concentration limit, biological bacteria micelle screening mother from the above conditions 2 to 5 years after the closure of the above landfill, generally choose 3~4 Year, the best choice is 4 years.

2COD concentration limit 1000~2000mg/l, ammonia nitrogen concentration limit 500~1000mg/l concentration limit, biological bacteria micelle screening mother from the above condition 6~9 years after the completion of the above-mentioned condition landfill, generally choose 7-8 Year, the best choice is 8 years.

3COD concentration limit 2000mg/over l, ammonia nitrogen concentration limit 1000mg/l or above concentration The limit value, the biological bacteria micelle screening mother from the above-mentioned conditional landfill 10 to 15 years after the closure of the field, generally choose 11 to 13 years, the best choice for 13 years.

Due to the long incubation time of the biological strains, it is possible to estimate the landfill time after the concentration increase according to the above data, that is, as the landfill time is longer, the components are relatively stable, and the catabolic ability of the microorganisms is also The stronger the strength, the stronger the ability to remove organic matter from wastewater. In the later stage, the full utilization of aging waste can be gradually realized to waste treatment.

(2) selecting organic matter screening materials of different longitudinal depths of the landfill for obtaining aerobic-biological phase micelles, facultative-biomass micelles, anaerobic-biomass micelles;

1 Aerobic-bio-phase bacteria micelle: The landfill is selected from a longitudinal depth of 1 to 4 meters of organic matter screening material, and is generally preferably 2 to 4 meters, and most preferably 4 meters.

2 facultative-bio-phase bacteria micelle: The landfill is selected from 4-6 meters longitudinal depth organic matter screening material for cultivation, generally preferably 5-6 meters, most preferably 6 meters.

3 Anaerobic-biological phase micelles: The organic matter-based screening materials of 6 to 10 meters in the landfill are selected for cultivation, and generally preferably 7 to 10 meters, and most preferably 10 meters.

(3) Screening of different particle size fillers according to the bed structure of the multi-biomicrobial micelle filter bed.

1 aerobic filter layer: screening coarse particle filter material from the obtained aerobic-bio-phase bacteria micelle, generally selecting particle size 4-6 cm, preferably 5-6 cm, optimally 5 cm, ensuring large filtration pores, ensuring Hydraulic load, intercepting large organic pollutants.

2 facultative filter layer: screening medium granule filter material from the obtained facultative-bio-bacterial micelles, generally selected particle size 2 ~ 4cm, preferably 3 ~ 4cm, optimally 4cm, extended residence time, further intercepting particle size The organic matter reduces the subsequent pollutant load.

3 Anaerobic filter layer: screening fine particle filter material from the obtained anaerobic-bio-phase bacteria micelle, generally selecting particle size of 0.5-2 cm, preferably 1-2 cm, optimally 2 cm, reducing porosity and prolonging denitrification retention Time to achieve efficient nitrogen removal.

(4) Screening process of multi-biomicrobial micelle packing

1 large block of garbage screening: use the crawler type excavator to determine the excavation depth according to the layer height of different multi-filler requirements, and carry out preliminary screening through the drum screen with a hole diameter of 8cm, and the large pieces of inorganic construction garbage, plastic bags, etc. After the material is removed, the material after screening is ready to enter the next step.

2 Screening of non-degradable materials such as plastic: According to the difference in density between plastic and organic materials, the broken plastic is removed by the air-selection system, and the material after screening enters the next step.

3 Ferromagnetic material screening: According to the physical properties of the material, the metal materials in which the structure has been destroyed are separated by magnetic separation, and the material after screening enters the next step.

4 Grain size selection: After the above sorting system, the metal, plastic and fiber fragments in the material are basically removed, and the remaining materials are basically organic humus and inorganic fine slag. The mixture of the two just ensures a large pore. Rate, ion exchange amount, number of microbial flora. Particle size screening is performed according to the requirements of different wastewater treatments and the particle size requirements of different bed structures.

(3) Selecting multiple biological phase microbial bacteria micelle implantation carrier

In order to facilitate the application of multi-biomicrobial micelles in practical engineering, it is necessary to realize a diversified combination of strains and filler carriers, and use biological exclusivity for carrier selection. The invention selects power plant ash, different size gravel, gravel, quartz sand, sludge ceramsite, calcium carbonate aggregate, PVC filler ball, generally preferred ash, gravel, gravel, quartz sand, PVC filler ball The best choice of ash, gravel, PVC filler balls.

The grading of the micelles to the carrier depends on the concentration of the organic matter in the wastewater.

1COD concentration limit 500～1000mg/l, ammonia nitrogen concentration limit 200～500mg/l concentration limit, bacteria micelle and ash residue, gravel, PVC filler ball carrier satisfying the aging time of parent material The gradation ratio (1 to 5): 1, generally preferably (2 to 4): 1.

2COD concentration limit 1000~2000mg/l, ammonia nitrogen concentration limit 500~1000mg/l concentration limit, the proportion of bacteria micelles and ash, gravel, PVC filler ball carrier that meet the aging time of the parent material (5~ 10): 1, generally preferred (6-8): 1.

3COD concentration limit 2000mg/above l, ammonia nitrogen concentration limit 1000mg/l concentration limit, the proportion of bacteria micelles and ash, gravel, PVC filler ball carrier meeting the maternal material aging time requirements (10~15) : 1, generally preferred (11 to 13): 1.

(4) Method of domestication of strains

The mode in which the wastewater to be treated is directly cultured is carried out by alternately mixing water and falling, and the dry-wet ratio is generally preferably (4 to 8): 1, most preferably (5 to 7): 1. The domestication process is as follows:

Day 1: Into the mixed dilution wastewater, the dilution ratio is in the form of wastewater: tap water = 1:5. The water intake is 10-20% of the design scale. The operation mode is continuous smoldering for 12 to 24 hours, and it is not drained.

The next day: venting, mixing and diluting wastewater, the dilution ratio is in accordance with the method of wastewater: tap water = 2:5. The water intake is 10-20% of the design scale. The operation mode is continuous smoldering for 12 to 24 hours, and it is not drained.

Day 3: Venting, mixing and diluting wastewater, the dilution ratio is in accordance with the method of wastewater: tap water = 3:5. The water intake is 10-20% of the design scale. The operation mode is continuous smoldering for 12 to 24 hours, and it is not drained.

Day 4: Venting, mixing and diluting wastewater, the dilution ratio is in accordance with the method of wastewater: tap water = 4:5. The water intake is 10-20% of the design scale. The operation mode is continuous smoldering for 12 to 24 hours, and it is not drained.

Day 5: venting, mixing and diluting wastewater, dilution ratio according to wastewater: tap water = 1:1 the way. The water intake is 10-20% of the design scale. The operation mode is continuous smoldering for 12 to 24 hours, and it is not drained.

The sixth day: the raw water to be treated, the influent amount is 40% of the design scale, the water is divided into 4 to 6 cycles, each time the water is 10~15%, the falling is 3~5h, the ventilation time is 2~4h, continuous drainage .

Day 7: The raw water to be treated is treated. The influent amount is 40% of the design scale. The water is divided into 4 to 6 cycles. Each time the water enters 10 to 15%, the water is dried for 3 to 5 hours, and the ventilation time is 2 to 4 hours. .

Day 8: The raw water to be treated is treated. The influent water is 60% of the design scale. The water is divided into 4 to 6 cycles. Each time the water is 10~15%, the drying is 3~5h, the ventilation time is 2~4h, and the drainage is continuous. .

The ninth day: the raw water to be treated, the water inflow is 60% of the design scale, the water is divided into 4 to 6 cycles, each time the water is 10 to 15%, the drying is 3 to 5 hours, the ventilation time is 2 to 4 hours, and the drainage is continuous. .

The tenth day: the raw water to be treated, the water inflow is 80% of the design scale, the water is divided into 4 to 6 cycles, each time the water is 10-15%, the drying is 3~5h, the ventilation time is 2~4h, continuous drainage .

The eleventh day: the raw water to be treated, the water intake is 80% of the design scale, the water is divided into 4 to 6 cycles, each time the water enters 10 to 15%, the water falls for 3 to 5 hours, and the ventilation time is 2 to 4 hours. drain.

The twelfth day: the raw water to be treated, the water intake is 100% of the design scale, the water is divided into 4 to 6 cycles, each time the water is 10 to 15%, the drying is 3 to 5 hours, and the ventilation time is 2 to 4 hours. drain. Domestication is completed and officially commissioned.

Claims (13)

1. A method for cultivating a multi-biomicrobial micelle for wastewater treatment, characterized in that the method comprises the following steps:

   (1) Establish multi-biological microbial bacteria micelle culture environment: select the culture field, carry out oxygen concentration gradient transformation, realize oxygen gradient change, artificially create aerobic, facultative and anaerobic culture environment, and construct a fully enclosed anatomical Oxygen culture field or semi-closed aerobic culture field, the culture environment should meet regional requirements, temperature requirements and organic matter content requirements;

   (2) Screening multi-microbial microbial cell micelles: In the first step, the multi-biomicrobial micelles of different years are selected according to the concentration of the wastewater to be treated to screen the mother body; the second step is to obtain aerobic-bio-phase gelatin. Group, facultative-bio-biomass micelles, anaerobic-bio-phase bacteria micelles, organic matter screening materials with different longitudinal depths of landfills are selected respectively; third step, according to multi-biomicrobial microbial gelatin filter bed The bed structure screens different particle size fillers, and the bed structure comprises one or more of an aerobic filter layer, a facultative filter layer, and an aerobic filter layer;

   (3) selecting a multi-biological microbial cellulite micelle carrier, and grading the multi-biological microbial cellulite group that meets the aging time requirement of the parent material in step (2) according to the concentration of the wastewater contaminant;

   (4) Domestication of strains by multi-biomicrobial micelle filter bed: direct culture with waste water to be treated, alternately by water distribution and drying, and the ratio of dry to wet is (4-8): 1, preferably ( 5-7): 1.
2. The method for cultivating a multi-biological microbial cell micelle for wastewater treatment according to claim 1, wherein the culture field selects a closed or semi-closed pit, and is filled into the pit by a certain amount. The organic matter of the component is preferably cultured in domestic garbage.
3. A method for cultivating a multi-biomicrobial micelle for wastewater treatment according to claim 1, wherein the culture field selects a domestic waste landfill.
4. A method for cultivating a multi-biomicrobial micelle for wastewater treatment according to any one of claims 1 to 3, characterized in that, in constructing a fully enclosed anaerobic culture field, the oxygen concentration gradient reforming method is: Landfill gas guides are collected to prevent combustion or explosion; according to the single-tube radiation area of 10-20 m ² and the longitudinal depth of 1 to 10 m, the air concentration gradient is divided, and the vertical perforated natural ventilation pipes are arranged at different depths; The diameter is 100-500mm, and there are vent holes with a diameter of 1-5cm around it.
5. A method for cultivating a multi-biomicrobial micelle for wastewater treatment according to any one of claims 1 to 3, characterized in that, in constructing a semi-closed aerobic culture field, the oxygen concentration gradient reforming method is: Using the design of the leakage flow of the leachate guide pipe and the temperature difference between the inside and outside of the landfill, the outside air of the pile naturally enters, and there is a certain aerobic area around the leachate discharge pipe and the vertical ventilation pipe, and the air is not diffused. The place is in an anaerobic state, directly achieving different oxygen concentration gradients.
6. A method for cultivating a multi-biological microbial cellulite for wastewater treatment according to any one of claims 1 to 3, characterized in that said region is specifically selected to select east of the Three Gorges of the Yangtze River with abundant annual precipitation. The middle and lower reaches of the coastal zone; the temperature requirement is: -10 ° C ~ 40 ° C; the organic matter content requirement is: the organic matter content is in the range of 20-50%.
7. A method for cultivating a multi-biological microbial cell micelle for wastewater treatment according to any one of claims 1 to 3, characterized in that: screening the parent body of the biological bacteria micelles of different years according to the concentration of the wastewater to be treated The method is:

   a. COD concentration is 500-1000mg/l, ammonia nitrogen concentration is 200-500mg/l, and the parent strain of biological bacteria micelles is from the culture field required by step (1) 2 to 5 years after the closure; preferably 3 to 4 years, most Preferably 4 years;

   b. COD concentration is 1000-2000mg/l, ammonia nitrogen concentration is 500-1000mg/l, and the parent strain of the biological bacteria micelle is from the culture field required to meet the step (1) 6 to 9 years after the closure; preferably 7 to 8 years, most Excellent 8 years;

   c. COD concentration limit 2000mg/l or more, ammonia nitrogen concentration limit 1000mg/l concentration limit, biological bacteria micelle screening mother from 10 to 15 years after closure of the field to meet the requirements of step (1) of the culture field; preferably 11 - 13 years, most preferably 13 years.
8. The method for cultivating a multi-biological microbial micelle for wastewater treatment according to claim 7, wherein the method for selecting organic matter-based screening materials having different longitudinal depths in the culture field is:

   a. aerobic-biological phase micelles: selecting a longitudinal depth of 1 to 4 meters of organic matter screening material; preferably 2-4 meters, most preferably 4 meters;

   b. facultative-bio-phase bacteria micelle: select 4~6 meters longitudinal depth organic matter screening material; preferably 5-6 meters, most preferably 6 meters;

   c. Anaerobic-biological phase micelles: 6 to 10 meters longitudinal depth organic matter screening material is selected; preferably 7 to 10 meters, most preferably 10 meters.
9. The method for cultivating a multi-biological microbial cell micelle for wastewater treatment according to claim 8, characterized in that the particle size screening of the organic matter-based screening materials of different longitudinal depths is selected according to the following manner, and the method is constructed. Multi-biomicrobial micelle filter bed: aerobic filter layer selects a coarse filter material having a particle diameter of 4 to 6 cm, preferably 5 to 6 cm, and most preferably 5 cm, from the obtained aerobic-biofilm micelle; The layer selects a medium-sized filter material having a particle diameter of 2 to 4 cm, preferably 3 to 4 cm, and most preferably 4 cm, from the obtained facultative-bio-biomass micelle; the anaerobic filter layer is selected from the obtained anaerobic-biofilm micelle The fine particle filter having a particle diameter of 0.5 to 2 cm, preferably 1 to 2 cm, and most preferably 2 cm.
10. A method for cultivating a multi-biological microbial cellulite for wastewater treatment according to any one of claims 1 to 3, characterized in that the method for sorting the organic-based screening material is:

    1 large block of garbage screening: use the crawler type excavator to determine the excavation depth according to the layer height of different multi-filler requirements, and carry out preliminary screening through the drum screen with a hole diameter of 8cm, which will block large inorganic construction garbage and plastic bag debris. Excluded, the material after screening is ready to enter the next link;

    2 Non-degradable plastic screening: According to the density difference between plastic and organic materials, the broken plastic is screened by the air-selection system, and the material after screening enters the next step;

    3 Ferromagnetic screening: According to the physical properties of the material, the ferromagnetic material is separated by magnetic separation, and the material after screening enters the next step;

    4 Particle size selection: According to the requirements of different wastewater treatment, and the particle size requirements of different bed structures, particle size screening.
11. Multi-biological microbial glue for wastewater treatment according to one of claims 1-3 The culture method of the group is characterized in that: the implantation carrier selects one or more of power plant ash, gravel, gravel, quartz sand, sludge ceramsite, calcium carbonate aggregate, and PVC filler ball.
12. The method for culturing a multi-biological microbial cell micelle for wastewater treatment according to claim 8 or 9, wherein the multi-biological microbial micelle and the implantation carrier are graded by:

    a. COD concentration of 500 ~ 1000mg / l, ammonia nitrogen concentration of 200 ~ 500mg / l, the screening of the requirements of the maternal material aging time requirements of multi-biomicrobial micelles and implantation carrier volume ratio (1 ~ 5): 1;

    b. COD concentration of 1000 ~ 2000mg / l, ammonia nitrogen concentration of 500 ~ 1000mg / l, the screening of the requirements of the maternal material aging time requirements of the multi-biomicrobial micelles and implantation carrier volume ratio (5 ~ 10): 1;

    c. COD concentration limit 2000mg / above l, ammonia nitrogen concentration limit 1000mg / l concentration limit, the screening of the multi-biomicrobial micelles and the implantation carrier volume ratio to meet the aging time of the parent material is (10 ~ 15) ):1.
13. A method for cultivating a multi-biological microbial cellulite for wastewater treatment according to any one of claims 1, 2, 3, 8, and 9, characterized in that the multi-biomicrobial micelle filter bed is used The domestication process of the strain includes: the first stage: the mixed dilution wastewater, the influent amount is 10-20% of the design scale, the operation mode is continuous smoldering exposure for 12-24 hours, undrained; the mixed dilution wastewater in the wastewater and tap water The dilution ratio is gradually increased to 1:1; each dilution ratio is one cycle, and the next dilution ratio of the mixed wastewater is vented before entering the water; the second stage: the raw water to be treated, the water intake is gradually increased from 40% of the design scale Up to 100%, 4-6 cycles into the water, each time into the water 10% ~ 15%, fall 3-5h, ventilation 2 ~ 4h, continuous drainage.

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