CN106520656A - Culture method of multi-biophase microbial zoogloea used for wastewater treatment - Google Patents
Culture method of multi-biophase microbial zoogloea used for wastewater treatment Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The invention relates to a culture method of multi-biophase microbial zoogloea used for wastewater treatment. The culture method comprises the following steps: establishing a bacterium culture environment for the multi-biophase microbial zoogloea; screening the multi-biophase microbial zoogloea; selecting an implantation carrier of the multi-biophase microbial zoogloea; and carrying out strain domesticating. According to the method, organic matter grows alternately in the aerobiotic, facultative and anaerobic states for a long term in the specific environment, and through the various effects in the aspects of physics and chemistry and biodegradation for many years, the multi-biophase microbial zoogloea with relatively stable property and composition is finally formed. The multi-biophase microbial zoogloea is rich in microbes huge in number and abundant in variety, the appearance of the multi-biophase microbial zoogloea has the characteristics of multiphase and multi-porosity and large superficial area, and the nutrient content is high. The analysis on the physical and chemical indexes of the zoogloea particles shows that zoogloea has the extremely excellent contaminant purifying function which is difficult to form under the natural condition, and thus the zoogloea belongs to an excellent medium for biological treatment of wastewater.
Description
Technical field
The invention belongs to ecological, environmental protective technical field, and in particular to a kind of many biofacies microbial bacterias for wastewater treatment
The cultural method of micelle.
Background technology
Micelle present in zoogloea of the prior art, such as activated sludge or biomembrance process are by the set on filler
The zoogloea of growth is that the microorganism sludge with metabolic activity grows what is formed in biological treatment system, this zoogloea
Inoculation environment is simple, without artificial screening step, implantation carrier is single and tames that controllability is poor, and the biofacies of formation are often single
One aerobic phase or anaerobism are equal.
Most crucial in the process of standard biologic filter bed is that bed fillers in microorganism, and filler of the prior art is according to property one
As be divided into inorganic filler, organic polymer filler and compounded mix three major types type.Conventional filler has as a drawback that:
1st, conventional filler cost of manufacture height, performance are low;
2nd, conventional filler specific surface area is low, and absorbability is poor;
3rd, conventional filler contaminant degradation ability, biofacies are unstable, and microbe species are single.
Above-mentioned filler is processing high concentrated organic wastewater, such as pollutant such as livestock breeding wastewater middle and high concentration COD, ammonia nitrogen
When it is less efficient.Therefore, need a kind of many biofacies microbial bacteria micelles suitable for high concentrated organic wastewater process of exploitation badly to fill out
Material and its cultural method, to meet increasingly strict emission request.
The content of the invention
For above-mentioned deficiency, the technical problem to be solved in the present invention is to provide a kind of being applied at high concentrated organic wastewater
The cultural method of many biofacies microbial bacteria micelle fillers of reason.
For achieving the above object, the present invention is achieved through the following technical solutions:
A kind of zoogleic cultural method of many biofacies microorganisms for wastewater treatment, the method are comprised the following steps:
(1) many biofacies microbial bacteria micelle cultivation environment are set up:Culture farm is selected, oxygen concentration gradients transformation is carried out,
Realize that oxygen gradient changes, artificially create the cultivation environment of aerobic, simultaneous oxygen and anaerobism, build totally enclosed type Anaerobic culturel field or half
Closed-type aerobic culture farm, cultivation environment should meet area requirement, temperature requirement and the content of organic matter and require;
(2) many biofacies microbial bacteria micelles are screened:The first step, chooses not the same year according to pending Wastewater Pollutant concentration
Many biofacies microbial bacteria micelles screening parents of part;Second step, is to obtain aerobic-biofacies zoogloea, simultaneous oxygen-biofacies bacterium
Micelle, anaerobism-biofacies zoogloea, chooses the organic matter class screening material of the different longitudinal direction depth of refuse landfill respectively;3rd
Step, screens different-grain diameter filler according to the bed structure of many biofacies microbial bacteria micelle filter beds, and the bed structure includes
One or more in aerobic filter layer, simultaneous oxygen filter layer, aerobic filter layer;
(3) many biofacies microbial bacteria micelle implantation carriers are selected, step (2) is screened according to Wastewater Pollutant concentration
The many biofacies microbial bacteria micelles for meeting the requirement of precursor material ageing time carry out level with implantation carrier and match somebody with somebody;
(4) using many biofacies microbial bacteria micelle filter beds to strain domestication:Directly cultivated using pending waste water, led to
Cross water distribution and the dry mode for alternateing that falls is carried out, psychrometric ratio is (4-8): 1, preferably (5-7): 1.
The culture farm selects the pit-hole of closed or semi-hermetic, by the organic matter for filling certain ingredients in pit-hole,
It is preferred that house refuse, is cultivated.
The culture farm selects household refuse landfill sites.
When building totally enclosed type Anaerobic culturel field, oxygen concentration gradients remodeling method is:Landfill gas guide is collected, and prevents combustion
Burn or explode;According to single tube 10~20m of swept area2, longitudinal 1~10m of depth divides air concentration gradient, in different depth cloth
Put vertically perforation gravity-flow ventilation pipe;The caliber of the gravity-flow ventilation pipe is 100-500mm, and it is saturating that its surrounding is provided with a diameter of 1-5cm
Pore.
When building semi-enclosed aerobic culture farm, oxygen concentration gradients remodeling method is:Using percolate discharge guiding pipe not
Flowing full designs and fills the internal-external temperature difference of heap body, the external air of heap is passed through naturally, in percolate discharge guiding pipe and vertically ventilation
There is certain aerobic region in pipeline, air spreads, and is directly realized by different oxygen
Gas Concentraton gradient.
Middle and lower reaches bank banding Plain to the east of the Yangtze River Gorges that the area requirement specially selects annual precipitation plentiful;
The temperature requirement is:- 10 DEG C~40 DEG C;The content of organic matter is required:The content of organic matter is in the range of 20-50%.
The biological zoogloea that different year is chosen according to pending Wastewater Pollutant concentration screens the method for parent:
A.COD 500~1000mg/l of concentration, 200~500mg/l of ammonia nitrogen concentration, biological zoogloea screening parent are self-styled
Meet the culture farm that step (1) is required within 2~5 years after;It is preferred that 3~4 years, most preferably 4 years;
B.COD 1000~2000mg/l of concentration, 500~1000mg/l of ammonia nitrogen concentration, biological zoogloea screening parent from
Meet the culture farm that step (1) is required within 6~9 years after closing;It is preferred that 7~8 years, it is optimum 8 years;
More than c.COD concentration limit 2000mg/l, ammonia nitrogen concentration limit value 1000mg/l concentrations above limit values, biological zoogloea
Screening parent met the culture farm that step (1) is required from 10~15 years after closing;It is preferred that 11-13, most preferably 13 years.
The organic matter class of the different longitudinal direction depth of selection culture farm sieves the method for material:
A. aerobic-biofacies zoogloea:Choose the longitudinal depth organic matter class screening material in 1~4 meter of training oxygen field;It is preferred that 2-4
Rice, most preferably 4 meters;
And oxygen-biofacies zoogloea b.:Choose the longitudinal depth organic matter class screening material of 4~6 meters of culture farm;It is preferred that 5-6
Rice, most preferably 6 meters;
C. anaerobism-biofacies zoogloea:Choose the longitudinal depth organic matter class screening material of 6~10 meters of culture farm;It is preferred that 7~
10 meters, most preferably 10 meters.
Preferably, the organic matter class screening material of difference longitudinal direction depth in such a way to choosing carries out particle diameter sieve
Choosing, builds many biofacies microbial bacteria micelle filter beds:Aerobic filter layer chooses grain from the aerobic-biofacies zoogloea for obtaining
The coarse grain filtrate of 4~6cm of footpath, preferably 5~6cm, most preferably 5cm;And oxygen filter layer is from the simultaneous oxygen-biofacies zoogloea for obtaining
Choose 2~4cm of particle diameter, the middle grain filtrate of preferably 3~4cm, most preferably 4cm;Anaerobism filter layer is from the anaerobism-biofacies bacterium for obtaining
Micelle chooses 0.5~2cm of particle diameter, the particulate filtrate of preferably 1~2cm, most preferably 2cm.
Organic matter class sieves the method for separating of material:
1. bulk rubbish sieve is removed:The floor height required according to different many fillers with crawler type digging machine, determines cutting depth, passes through
The rotary screen in aperture 8cm apertures is tentatively sieved, and the inorganic building waste of bulk, plastic bag debris is rejected, material after sieve
It is prepared to enter into next link;
2. nondegradable plastic screen is removed:It is different in nature with the density contrast of organic matter material according to plastics, will by aidr bells blast system
Broken plastic screen is removed, and after sieve, material enters next link;
3. ferromagnetic is screened out:According to the physical attribute of material, ferromagnetic is separated by magnetic separation, after sieve, material is entered
Next link;
4. granularity is selected:According to the requirement of different wastewater treatments, and the Grained Requirements of different bed structures, carry out particle diameter
Screening.
The implantation carrier selects cinder of electric power plant, rubble, gravel, quartz sand, sludge ceramsite, Calcium Carbonate aggregate, PVC fillers
One or more in ball.
Many biofacies microbial bacteria micelles with the level of implantation carrier with mode are:
A.COD 500~1000mg/l of concentration, 200~500mg/l of ammonia nitrogen concentration, screening meet precursor material it is aging when
Between many biofacies microbial bacteria micelles for requiring be (1~5) with implantation carrier bulk ratio: 1;
B.COD 1000~2000mg/l of concentration, 500~1000mg/l of ammonia nitrogen concentration, screening to meet precursor material aging
Many biofacies microbial bacteria micelles of time requirement are (5~10) with implantation carrier bulk ratio: 1;
C.COD concentration limit 2000mg/ above l, ammonia nitrogen concentration limit value 1000mg/l concentrations above limit values, the satisfaction of screening
Many biofacies microbial bacteria micelles that precursor material ageing time is required are (10~15) with implantation carrier bulk ratio: 1.
The process tamed to strain using many biofacies microbial bacteria micelle filter beds is included:First stage:Enter mixed
Dilution waste water is closed, inflow for design scale 10~20%, the method for operation are 12~24h of continuous vexed exposure, not draining;It is described mixed
In closing dilution waste water, waste water is stepped up to 1: 1 with the dilution ratio of tap water;Each dilution ratio is circulated for one, next
Before the composite waste water inlet of dilution ratio, emptying;Second stage:Enter pending raw water, inflow by design scale 40% by
Step brings up to 100%, point 4-6 cycle water inlet, water inlet 10%~15% every time, and fall dry 3-5h, and divulge information 2~4h, continuous drainage.
Beneficial effects of the present invention are:" many biofacies microbial bacteria micelles " filler be organic matter in certain circumstances, it is long
Phase in aerobic, and oxygen, alternating growth under anaerobic state, through physics for many years, chemistry and the multinomial effect of biodegradation, finally
The metastable class soil unconsolidated material of property and component of formation.In zoogloea, microorganism is enriched, and quantity species is various,
Outward appearance possesses the big feature of multiphase concrete dynamic modulus rate and surface area, the advantages of nutritious content of material is high.By to zoogloea granule
Physical and chemical index is analyzed, and is shown which has and is difficult to pollutant purification performance to be formed, extremely excellent under field conditions (factors), is
Excellent biological wastewater treatment medium.
Environment is inoculated with by the zoogleic cultivation of many biofacies microorganisms to set up, many biofacies microbial bacteria micelle screenings,
Zoogloea implantation carrier is selected, and biological inoculum acclimation combines and realizes to the zoogleic culture of many biofacies microorganisms, with
Meet demand of the wastewater treatment to microorganism fungus kind.By many biofacies microbial bacteria micelle strong adaptabilities of the method culture,
It is stable, realize efficient, the low-cost processes of high concentrated organic wastewater.
Description of the drawings
Fig. 1 is many biofacies microbial bacteria micelle Anaerobic culturel field structure schematic diagrams;
Fig. 2 is the aerobic culture farm structural representation of many biofacies microbial bacteria micelles.
Specific embodiment
In line with the treatment of wastes with processes of wastes against one another, the principle of recycling, the corresponding refuse landfill of prioritizing selection of the present invention are cultivated
Condition transform, meet the culture of many biofacies microbial bacteria micelles to temperature, oxygen concentration, organic nutrient substance demand;Train simultaneously
The foster cycle is also the key for choosing many biofacies microbial bacteria micelle fillers.Through aerobic, simultaneous oxygen, the oxygen concentration gradients of anaerobism
Change, a kind of capacity of resisting impact load formed under the long-term domestication of high concentration organic solution is rough compared with strong, conditions of existence, degraded
A very competent class soil type microbiologic population.
According to same nitrated in position denitrification and the principle of Anammox, with screening and culturing after " many biofacies microbial bacteria glue
Group " is substrate, by the cation exchange capacity and microorganism affinity of its super large, is formed and is adapted to many biofacies strain existence
Microecological environment, efficiently remove high concentrated organic wastewater particularly livestock breeding wastewater middle and high concentration COD, ammonia nitrogen etc. so as to reach
The purpose of organic pollution.
Many biofacies microbial bacteria micelles are completely different with the wastewater treatment microbial cultivation vaccination ways of routine.Conventional
Microbial inoculant is usually, by the way of microorganism colonization, exactly to make the microorganism sludge with metabolic activity in biological treatment
The process of set growth on filler in system.Many biofacies microbial bacteria micelles are to adopt directly to mix in proportion with carrier mass
Close, microorganism culturing is carried out by way of additional organic matter solution is as nutritional solution inoculation, formed with aerobic microbiological, nitre
Change antibacterial, denitrifying bacteria, the antibacterial micelle of one class of anaerobic ammonia oxidizing bacteria.
(1) many biofacies microbial bacteria micelle cultivation environment are set up:Culture farm is selected, oxygen concentration gradients transformation is carried out,
Realize that oxygen gradient changes, artificially create the cultivation environment of aerobic, simultaneous oxygen and anaerobism, build totally enclosed type Anaerobic culturel field or half
Closed-type aerobic culture farm, cultivation environment should meet area requirement, temperature requirement and the content of organic matter and require.
The microorganism species of wastewater treatment include many types, according to its demand to oxygen can be divided into aerobic microbiological,
Amphimicrobe, obligate anaerobe.Many biofacies microbial bacteria micelles include the micro- of above-mentioned three kinds of different shapes
Biological flora.Due to the demand concentration to oxygen it is different, this be accomplished by cultivating microorganism steric requirements can multiple realization it is different
Concentraton gradient.The present invention have selected totally enclosed type Anaerobic culturel field, semi-enclosed aerobic culture farm, each by changing culture space
The oxygen concentration in region, realizes that oxygen gradient changes, and artificially creates aerobic, simultaneous oxygen, the cultivation environment of anaerobism.Cultivation environment this
Bright prioritizing selection household refuse landfill sites, and transformed according to above-mentioned cultivation environmental requirement.
Anaerobic culturel field builds:House refuse is more than impervious barrier to be carried out being layered nature stacking, successively covers, finally
Close.This type culture farm feature is, before early stage does not carry out layering grave, due to the entrance of new refuse so that system environment
In it is aerobic and and oxygen condition, as the presence culture part aerobic microbiological of Organic substance decomposes to Organic substance, formed
A certain amount of aerobic microbiological group.But with the increase of landfill height, bottom space is progressively transitioned into anaerobism state, part
Microorganism conversion is anaerobe.It is honored as a queen by stratified soil, whole system space defines closed anaerobism space, originally residual
The oxygen deposited can also disappear totally, cannot thus realize various demands of the microorganism to oxygen concentration, be in this present invention such as
Fruit chooses anaerobism cultivation place, it will before strain screening, cultivation field carries out the transformation of oxygen concentration gradients.Reforming mode is as follows:
1. landfill gas guide is collected, and is prevented from burning or is exploded.2. air delivery duct is set, according to single tube 10~20m of swept area2,
Longitudinal 1~10m of depth divides air concentration gradient, realizes that the Concentraton gradient on solid space affects.The area for such as selecting factory is
10000m2, then needing to arrange 500~1000 longitudinal tracheas in plane space, selected scenes depth 15m then should be in 1~10m not
Trachea is arranged with level different depth, it is ensured that the Concentraton gradient change of oxygen can be realized under upper.3. longitudinal trachea depth
Interlaced arrangement is needed, deep mixed, you can to simulate under different Oxygen Conditions, the change of microbe species or alternately conversion,
To guarantee that microorganism all keeps certain biological activity under different Oxygen Conditions.
Aerobic culture farm builds:In order to ensure the extensive replication application of many biofacies microbial bacteria micelle fillers, to good
The zoogleic culture of many biofacies microorganisms of oxygen culture farm is also the emphasis of the present invention.Aerobic design principle is supplied without power
Oxygen, but designed using the slack flow of percolate discharge guiding pipe, using the internal-external temperature difference of landfill heap body, the external air of heap is led to naturally
Enter, there is certain aerobic region around leachate collection pipe and vertical ventilation shaft, rubbish herein carries out aerobic decomposition,
Air spreads.Relative to above-mentioned anaerobism cultivation field, accurate aerobic cultivation field accelerates oozes
The discharge of filtrate, it is suppressed that the generation of the gas such as methane and hydrogen sulfide, accelerates Organic substance stabilisation process, reduces dirt in percolate
Dye thing concentration.The space oxygen concentration gradients of on-site can be directly realized by by this kind of mode, aerobic, simultaneous oxygen, anaerobism is realized
The common survival of microorganism, is easy to the zoogleic screening of many biofacies microorganisms.
The adopted cultivation place of the present invention utilizes original place and facility as far as possible, as microorganism selects special to Organic substance
Property, corresponding place, the refuse landfill of prioritizing selection corresponding area of the present invention to be selected according to different situations.Due to differently
The productive life custom diversity of area people is larger, causes the waste component of various places also to make a big difference.Such as northern area,
Due to cold weather, coal stove for heating will produce substantial amounts of coal ash, thus it is most in component of refuse be exactly that cinder etc. is inorganic
Material;Southern area, warmer climate, dietary structure are enriched, and changing food waste is more, is the organic wastes such as kitchen in rubbish mostly,
Nutrient substance enriches.Compare more southern refuse landfill and be more suitable for the zoogleic training of many biofacies microorganisms in the present invention
Support.In In Middle And Lower Reaches of Changjiang River, this area can not only meet many biofacies for land-fill areas position selected by the present invention
Requirement of the microbial bacteria micelle to organic matter in rubbish, can also meet temperature change requirement.
1. regional choice:Middle and lower reaches bank banding Plain to the east of the Yangtze River Gorges.North meets Huai Yangshan, and south connects Jiangnan Qiuling.
Physical features is low flat, and the big portion of ground level is below 50 meters.Plain of Middle Reaches includes that Jianghan plain of Hubei province, Hunan Dongtinghu Plain (are collectively referred to as
Lianghu Plain) and Poyang Lake in Jiangxi province Plain.Downstream Plain includes Changjiang River of Anhui coastal plain and Chaohu Plain and Jiangsu, Zhejiang
Yangtze River Delta between river, Shanghai.The region annual precipitation is more abundant, it is possible to achieve percolate is to microorganism species
Intermittent impacts, are conducive to the anti-load impact ability of cultivating microorganism.
2. temperature is selected:- 10~40 DEG C, the temperature range for maintaining microorganism normal biological activity is 15~35 DEG C or so,
By prolonged biological domestication so as to which temperature tolerance is progressively strengthened, but limiting temperature limit value can not be broken through, otherwise micro- life
Thing is by death or loses activity completely.Generally preferably 0~35 DEG C, most preferably preferably 15~35 DEG C.
3. the content of organic matter:In order to maintain the metabolism of microorganism its life, organic matter as nutrient substance is must
Indispensable, the application claims content of organic matter is between 20~50%, it is generally preferable to 20~40%, most preferably preferably 20~
30%.Will excessively bring Secondary Organic thing to pollute, improve the concentration of the COD and ammonia nitrogen of Body filler leachate, it is very few, no
Beneficial to the culture of microorganism.
(2) many biofacies microbial bacteria micelles are screened:
(1) many biofacies microbial bacteria micelle screening mothers of different year are chosen according to pending Wastewater Pollutant concentration
Body;
1. 500~1000mg/l of COD concentration limits, ammonia nitrogen concentration limit value 200~500mg/l concentration limits, biological bacteria glue
Group's screening parent is typically chosen 3~4 years, optimal choice 4 years from the above-mentioned condition refuse landfill of 2~5 years after closing.
2. 1000~2000mg/l of COD concentration limits, ammonia nitrogen concentration limit value 500~1000mg/l concentration limits, biological bacteria
Micelle screens parent from the above-mentioned condition refuse landfill of 6~9 years after closing, is typically chosen 7~8 years, optimal choice 8 years.
3. COD concentration limits 2000mg/ above l, ammonia nitrogen concentration limit value 1000mg/l concentrations above limit values, biological zoogloea
Screening parent is typically chosen 11~13 years, optimal choice 13 years from the above-mentioned condition refuse landfill of 10~15 years after closing.
As biological inoculum is cultivated, the time is longer, can carry out filling time lengthening after concentration growth according to above-mentioned data
Estimate, i.e., with landfill the time it is longer, each component is also just relatively stable, and the catabolism ability of microorganism is also stronger, right
Organic matter removal ability in waste water is also stronger, and the later stage can be done step-by-step the complete utilization of aging rubbish, the treatment of wastes with processes of wastes against one another.
(2) rubbish is chosen for obtaining aerobic-biofacies zoogloea, simultaneous oxygen-biofacies zoogloea, anaerobism-biofacies zoogloea
The organic matter class screening material of the different longitudinal direction depth of rubbish landfill yard;
1. aerobic-biofacies zoogloea:Choosing the longitudinal depth organic matter class screening material of 1~4 meter of refuse landfill is carried out
Culture, it is generally preferable to 2~4 meters, most preferably 4 meters.
And oxygen-biofacies zoogloea 2.:Choosing the longitudinal depth organic matter class screening material of 4~6 meters of refuse landfill is carried out
Culture, it is generally preferable to 5~6 meters, most preferably 6 meters.
3. anaerobism-biofacies zoogloea:Choosing the longitudinal depth organic matter class screening material of 6~10 meters of refuse landfill is carried out
Culture, it is generally preferable to 7~10 meters, most preferably 10 meters.
(3) different-grain diameter filler is screened according to the bed structure of many biofacies microbial bacteria micelle filter beds.
1. aerobic filter layer:Screen coarse grain filtrate from the aerobic-biofacies zoogloea for obtaining, it is general choose particle diameter 4~
6cm, preferably 5~6cm, optimum is 5cm, it is ensured that larger filtration hole, it is ensured that hydraulic load, retains larger organic contamination
Thing.
And oxygen filter layer 2.:Grain filtrate in screening from the simultaneous oxygen-biofacies zoogloea for obtaining, it is general choose particle diameter 2~
4cm, preferably 3~4cm, optimum is 4cm, extends the time of staying, further retains the general Organic substance of granularity, mitigates subsequent contamination
Thing load.
3. anaerobism filter layer:Particulate filtrate is screened from the anaerobism-biofacies zoogloea for obtaining, it is general to choose particle diameter 0.5
~2cm, preferably 1~2cm, optimum is 2cm, reduces porosity, extends the denitrification time of staying, realize efficient denitrification.
(4) many biofacies microbial bacteria micelle packing screening processes
1. bulk rubbish sieve is removed:The floor height required according to different many fillers with crawler type digging machine, determines cutting depth, passes through
The rotary screen in aperture 8cm apertures is tentatively sieved, and the debris such as the inorganic building waste of bulk, plastic bag is rejected, thing after sieve
Material is prepared to enter into next link.
2. the nondegradable material such as plastics is screened out:It is different in nature with the density contrast of organic matter material according to plastics, by selecting crude drugs with winnower
Broken plastics are shone and are removed by system, and after sieve, material enters next link.
3. ferromagnetic material is screened out:According to the physical attribute of material, by magnetic separation by the metal of wherein structural deterioration
Material is separated, and after sieve, material enters next link.
4. granularity is selected:After crossing above-mentioned separation system, the metal, plastics, fiber relic in material is removed substantially, remaining
Material is essentially organic humus and the tiny clinker of inorganic matters, and both mixing ensure that larger porosity, ion just
Exchange capacity, microorganism species quantity.According to the requirement of different wastewater treatments, and the Grained Requirements of different bed structures, carry out
Particle diameter is screened.
(3) many biofacies microbial bacteria micelle implantation carriers are selected
For the ease of application of many biofacies microbial bacteria micelles in Practical Project, it is necessary to realize strain and filling carrier
Diversification combination, carry out carrier selection using biological exclusiveness.The present invention have selected cinder of electric power plant, different-grain diameter rubble, gravel
Stone, quartz sand, sludge ceramsite, Calcium Carbonate aggregate, PVC filling balls, ordinary priority select lime-ash, rubble, gravel, quartz sand, PVC
Filling ball, optimal choice lime-ash, rubble, PVC filling balls.
Zoogloea depends on concentration of organic wastewater with the level of carrier with mode.
1. 500~1000mg/l of COD concentration limits, ammonia nitrogen concentration limit value 200~500mg/l concentration limits, meet parent
The zoogloea of material ageing time requirement and lime-ash, rubble, PVC filling balls carrier-grade are with ratio (1~5): 1, it is generally preferable to (2
~4): 1.
2. 1000~2000mg/l of COD concentration limits, ammonia nitrogen concentration limit value 500~1000mg/l concentration limits, meet female
The zoogloea of body material ageing time requirement and lime-ash, rubble, PVC filling balls carrier-grade are with ratio (5~10): 1, it is generally preferable to
(6~8): 1.
3. COD concentration limits 2000mg/ above l, ammonia nitrogen concentration limit value 1000mg/l concentrations above limit values, meet parent thing
The zoogloea of material ageing time requirement and lime-ash, rubble, PVC filling balls carrier-grade are with ratio (10~15): 1, it is generally preferable to (11
~13): 1.
(4) strain domestication mode
The pattern directly cultivated using pending waste water;Carried out by way of water distribution and dry the alternateing that fall, psychrometric ratio
It is generally preferred to (4~8): 1, most preferably (5~7): 1.Domestication process is as follows:
First day:Enter mixed diluting waste water, dilution ratio is according to waste water: tap water=1: 5 mode.Inflow is design
The 10~20% of scale.The method of operation is 12~24h of continuous vexed exposure, not draining.
Second day:Emptying, enters mixed diluting waste water, and dilution ratio is according to waste water: tap water=2: 5 mode.Inflow
For the 10~20% of design scale.The method of operation is 12~24h of continuous vexed exposure, not draining.
3rd day:Emptying, enters mixed diluting waste water, and dilution ratio is according to waste water: tap water=3: 5 mode.Inflow
For the 10~20% of design scale.The method of operation is 12~24h of continuous vexed exposure, not draining.
4th day:Emptying, enters mixed diluting waste water, and dilution ratio is according to waste water: tap water=4: 5 mode.Inflow
For the 10~20% of design scale.The method of operation is 12~24h of continuous vexed exposure, not draining.
5th day:Emptying, enters mixed diluting waste water, and dilution ratio is according to waste water: tap water=1: 1 mode.Inflow
For the 10~20% of design scale.The method of operation is 12~24h of continuous vexed exposure, not draining.
6th day:Enter pending raw water, inflow for design scale 40%, point 4~6 cycle water inlets are intake every time
10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.
7th day:Enter pending raw water, inflow for design scale 40%, point 4~6 cycle water inlets are intake every time
10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.
8th day:Enter pending raw water, inflow for design scale 60%, point 4~6 cycle water inlets are intake every time
10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.
9th day:Enter pending raw water, inflow for design scale 60%, point 4~6 cycle water inlets are intake every time
10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.
Tenth day:Enter pending raw water, inflow for design scale 80%, point 4~6 cycle water inlets are intake every time
10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.
11st day:Enter pending raw water, inflow for design scale 80%, point 4~6 cycle water inlets enter every time
Water 10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.
12nd day:Enter pending raw water, inflow for design scale 100%, point 4~6 cycle water inlets enter every time
Water 10~15%, fall dry 3~5h, 2~4h of ventilation time, continuous drainage.Domestication is completed, formal trial operation.
Claims (13)
1. a kind of zoogleic cultural method of many biofacies microorganisms for wastewater treatment, it is characterised in that the method includes
Following steps:
(1)Set up many biofacies microbial bacteria micelle cultivation environment:Culture farm is selected, oxygen concentration gradients transformation is carried out, is realized
Oxygen gradient changes, and artificially creates the cultivation environment of aerobic, simultaneous oxygen and anaerobism, builds totally enclosed type Anaerobic culturel field or semiclosed
The aerobic culture farm of formula, cultivation environment should meet area requirement, temperature requirement and the content of organic matter and require;
(2)Screen many biofacies microbial bacteria micelles:The first step, chooses different year according to pending Wastewater Pollutant concentration
Many biofacies microbial bacteria micelles screen parent;Second step, is to obtain aerobic-biofacies zoogloea, simultaneous oxygen-biofacies bacterium glue
Group, anaerobism-biofacies zoogloea, choose the organic matter class screening material of the different longitudinal direction depth of refuse landfill respectively;3rd step,
Different-grain diameter filler is screened according to the bed structure of many biofacies microbial bacteria micelle filter beds, the bed structure includes aerobic
One or more in filter layer, simultaneous oxygen filter layer, aerobic filter layer;
(3)Many biofacies microbial bacteria micelle implantation carriers are selected, according to Wastewater Pollutant concentration by step(2)The satisfaction of screening
Many biofacies microbial bacteria micelles that precursor material ageing time is required carry out level with implantation carrier and match somebody with somebody;
(4)Using many biofacies microbial bacteria micelle filter beds to strain domestication:Directly cultivated using pending waste water, by matching somebody with somebody
Water and the dry mode for alternateing that falls are carried out, and psychrometric ratio is(4-8):1, preferably(5-7):1.
2. a kind of zoogleic cultural method of many biofacies microorganisms for wastewater treatment as claimed in claim 1, which is special
Levy and be:The culture farm selects the pit-hole of closed or semi-hermetic, by the organic matter for filling certain ingredients in pit-hole, excellent
House refuse is selected, is cultivated.
3. a kind of zoogleic cultural method of many biofacies microorganisms for wastewater treatment as claimed in claim 1, which is special
Levy and be:The culture farm selects household refuse landfill sites.
4. a kind of zoogleic culture side of many biofacies microorganisms for wastewater treatment as described in one of claim 1-3
Method, it is characterised in that:When building totally enclosed type Anaerobic culturel field, oxygen concentration gradients remodeling method is:Landfill gas guide is collected,
Prevent from burning or explode;According to single tube 10 ~ 20m of swept area2, longitudinal 1 ~ 10m of depth divides air concentration gradient, different deep
The vertical perforation gravity-flow ventilation pipe of degree arrangement;The caliber of the gravity-flow ventilation pipe is 100-500mm, and its surrounding is provided with a diameter of 1-
5cm air-vents.
5. a kind of zoogleic culture side of many biofacies microorganisms for wastewater treatment as described in one of claim 1-3
Method, it is characterised in that:When building semi-enclosed aerobic culture farm, oxygen concentration gradients remodeling method is:Using percolate guide
The slack flow design of pipe and the internal-external temperature difference of heap body is filled, the external air of heap is passed through naturally, in percolate discharge guiding pipe and perpendicular
There is certain aerobic region in direct ventilation pipeline, air spreads, is directly realized by not
Same oxygen concentration gradients.
6. a kind of zoogleic culture side of many biofacies microorganisms for wastewater treatment as described in one of claim 1-3
Method, it is characterised in that:The middle and lower reaches littoral zone to the east of the Yangtze River Gorges that the area requirement specially selects annual precipitation plentiful
Shape Plain;The temperature requirement is:-10℃~40℃;The content of organic matter is required:The content of organic matter is in 20-50% scopes
It is interior.
7. a kind of zoogleic culture side of many biofacies microorganisms for wastewater treatment as described in one of claim 1-3
Method, it is characterised in that:The method that the biological zoogloea that different year is chosen according to pending Wastewater Pollutant concentration screens parent
For:
A. 500 ~ 1000mg/l of COD concentration, 200 ~ 500mg/l of ammonia nitrogen concentration, biological zoogloea screening parent from after closing 2 ~
5 years meet step(1)The culture farm of requirement;It is preferred that 3 ~ 4 years, most preferably 4 years;
B. 1000 ~ 2000mg/l of COD concentration, 500 ~ 1000mg/l of ammonia nitrogen concentration, biological zoogloea screen parent after closing
6 ~ 9 years meet step(1)The culture farm of requirement;It is preferred that 7 ~ 8 years, it is optimum 8 years;
C. more than COD concentration limits 2000mg/ l, ammonia nitrogen concentration limit value 1000mg/l concentrations above limit values, biological zoogloea sieve
Parent is selected to meet step from 10 ~ 15 years after closing(1)The culture farm of requirement;It is preferred that 11-13, most preferably 13 years.
8. a kind of zoogleic cultural method of many biofacies microorganisms for wastewater treatment as claimed in claim 7, which is special
Levy and be:The organic matter class of the different longitudinal direction depth of selection culture farm sieves the method for material:
A. aerobic-biofacies zoogloea:Choose the longitudinal depth organic matter class screening material of 1 ~ 4 meter of culture farm;It is preferred that 2-4 rice, most
It is preferred that 4 meters;
And oxygen-biofacies zoogloea b.:Choose the longitudinal depth organic matter class screening material of 4 ~ 6 meters of culture farm;It is preferred that 5-6 rice, most
It is preferred that 6 meters;
C. anaerobism-biofacies zoogloea:Choose the longitudinal depth organic matter class screening material of 6 ~ 10 meters of culture farm;It is preferred that 7 ~ 10 meters,
Most preferably 10 meters.
9. a kind of zoogleic cultural method of many biofacies microorganisms for wastewater treatment as claimed in claim 8, which is special
Levy and be:The organic matter class screening material of the difference longitudinal direction depth to choosing carries out particle diameter screening in such a way, builds many
Biofacies microbial bacteria micelle filter bed:Aerobic filter layer chooses 4 ~ 6cm of particle diameter from the aerobic-biofacies zoogloea for obtaining,
It is preferred that the coarse grain filtrate of 5 ~ 6cm, most preferably 5cm;And oxygen filter layer choose from the simultaneous oxygen-biofacies zoogloea for obtaining particle diameter 2 ~
The middle grain filtrate of 4cm, preferably 3 ~ 4cm, most preferably 4cm;Anaerobism filter layer chooses particle diameter from the anaerobism for obtaining-biofacies zoogloea
The particulate filtrate of 0.5 ~ 2cm, preferably 1 ~ 2cm, most preferably 2cm.
10. a kind of zoogleic culture side of many biofacies microorganisms for wastewater treatment as described in one of claim 1-3
Method, it is characterised in that:Organic matter class sieves the method for separating of material:
1. bulk rubbish sieve is removed:The floor height required according to different many fillers with crawler type digging machine, determines cutting depth, by hole
The rotary screen in footpath 8cm apertures is tentatively sieved, and the inorganic building waste of bulk, plastic bag debris are rejected, and after sieve, material is accurate
It is standby to enter next link;
2. nondegradable plastic screen is removed:According to plastics and the density contrast opposite sex of organic matter material, broken by aidr bells blast system
Broken plastic screen is removed, and after sieve, material enters next link;
3. ferromagnetic is screened out:According to the physical attribute of material, ferromagnetic is separated by magnetic separation, under after sieve, material is entered
One link;
4. granularity is selected:According to the requirement of different wastewater treatments, and the Grained Requirements of different bed structures, carry out particle diameter sieve
Choosing.
A kind of 11. zoogleic culture sides of many biofacies microorganisms for wastewater treatment as described in one of claim 1-3
Method, it is characterised in that:The implantation carrier select cinder of electric power plant, rubble, gravel, quartz sand, sludge ceramsite, Calcium Carbonate aggregate,
One or more in PVC filling balls.
A kind of 12. zoogleic cultural methods of many biofacies microorganisms for wastewater treatment as claimed in claim 8 or 9,
It is characterized in that:Many biofacies microbial bacteria micelles with the level of implantation carrier with mode are:
A. 500 ~ 1000mg/l of COD concentration, 200 ~ 500mg/l of ammonia nitrogen concentration, screening meet the requirement of precursor material ageing time
Many biofacies microbial bacteria micelles with implantation carrier bulk ratio be(1~5):1;
B. 1000 ~ 2000mg/l of COD concentration, 500 ~ 1000mg/l of ammonia nitrogen concentration, the precursor material ageing time that meets of screening will
The many biofacies microbial bacteria micelles asked with implantation carrier bulk ratio are(5~10):1;
C. COD concentration limits 2000mg/ above l, ammonia nitrogen concentration limit value 1000mg/l concentrations above limit values, the satisfaction of screening are female
Body material ageing time require many biofacies microbial bacteria micelles with implantation carrier bulk ratio be(10~15):1.
A kind of 13. many biofacies microorganisms for wastewater treatment as described in one of claim 1,2,3,8,9 are zoogleic
Cultural method, it is characterised in that:The process tamed to strain using many biofacies microbial bacteria micelle filter beds is included:The
One stage:Enter mixed diluting waste water, inflow for design scale 10 ~ 20%, the method for operation are 12 ~ 24h of continuous vexed exposure, do not arrange
Water;In the mixed diluting waste water, waste water is stepped up to 1 with the dilution ratio of tap water:1;Each dilution ratio is one
Circulation, before the composite waste water inlet of next dilution ratio, emptying;Second stage:Enter pending raw water, inflow is by design scale
40% step up 100%, point 4-6 cycle water inlet intakes 10% ~ 15% every time, and fall dry 3-5h, and divulge information 2 ~ 4h, continuous row
Water.
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