CN101979496B - Domestication method for microcoleus vaginatus and application thereof - Google Patents
Domestication method for microcoleus vaginatus and application thereof Download PDFInfo
- Publication number
- CN101979496B CN101979496B CN 201010263525 CN201010263525A CN101979496B CN 101979496 B CN101979496 B CN 101979496B CN 201010263525 CN201010263525 CN 201010263525 CN 201010263525 A CN201010263525 A CN 201010263525A CN 101979496 B CN101979496 B CN 101979496B
- Authority
- CN
- China
- Prior art keywords
- algae
- domestication
- little
- saltings
- little sheath
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a domestication method for microcoleus vaginatus and application thereof. The method comprises the following steps of: preparing a special culture medium, performing domestications such as salt tolerance domestication, nitrogen-phosphorus content reducing domestication, adaptability to water to be treated and the like on the screened algae seeds, introducing the algae seeds into a saline-alkali land after gradual amplification culture, propagating a great amount of microcoleus vaginatus by using photosynthesis, wherein the microcoleus vaginatus absorbs and fixes nitrogen and phosphorus elements in the atmosphere and the water source; and draining the microcoleus vaginatus culture water after propagation, and depositing the microcoleus vaginatus in the soil, wherein the microcoleus vaginatus contains a large amount of elements such as nitrogen, phosphorus and the like. After one-time treatment of the method, the nitrogen in the soil is increased by 19 to 22 percent, the phosphorus is increased by 13 to 15 percent, and the full salt quantity is reduced by 25 to 30 percent; and the method avoids nutrient element loss caused by a washing method, avoids hardening of the soil and secondary pollution of the water source and the environment due to the application of fertilizers, can gradually enhance the soil fertility by multiple times of treatment, can accelerate soil recovery, and finally fulfills the purpose of improving the ecological environment.
Description
Technical field
The invention discloses a kind of method of utilizing fixed nitrogen blue-green algae improvement saltings, increasing soil fertility, particularly a kind of method of utilizing little sheath algae to handle the saltings.
Background technology
The saltings distributes very wide all over the world, spread all over more than 30 countries in six continents.Mainly be distributed in countries such as India, Pakistan, Russia, South Africa, about 9.56 hundred million hectares of the total area.Wherein India and Pakistan are two state-owned 8,090,000 hectares, 7,500,000 hectares of Russia.The saltings area of Western United States accounts for about half of irrigation district area, and reduce by 25% or more because of saline and alkaline output every year.The soil salinization is the important component part that constitutes desertification of land, and local husbandry production in serious threat, the solution of a series of significant problems of improvement of the development and prosperity of national economy and ecotope.For a long time, scientist has carried out many-sided exploration and research to the improvement in saltings both at home and abroad.In 20 beginnings of the century characteristic is learned in regional distribution, formation reason, evolution process and the generation in saltings and carried out many-sided research.Set up the anti-salt engineering system (digging canal, open drain, hidden pipe, well-digging water lift etc.) of irrigation, water quality, antiseepage and irrigation and drainage centered by hydro project, soil improvement the thirties.Over nearly 40 years, some economic flourishing countries of USSR (Union of Soviet Socialist Republics), the U.S. are with physics, chemistry and agricultural measures improvement saltings, as use industry byproduct (slag, gypsum) and make soil improvement agent, and it is saline and alkaline to join flushing with clean water, to improve soil physico-chemical property.But these measures need bigger material resources, and financial resources and a large amount of fresh water also are difficult to widespread use.The fifties in last century USSR (Union of Soviet Socialist Republics) just filter out some saline alkali tolerant plants, as alkali wheatgrass, Itanlian rye, cogongrass, river grain uncle clover etc.The U.S. once selected salt-tolerant plants such as shore rattan, heavy foliage shore rattan, still was in conceptual phase at present, was not applied to as yet produce.China has all kinds ofly is rich in saline and alkaline land area and has 3,460 ten thousand square kilometres approximately, mainly be distributed in Xinjiang, Gansu, Ningxia, the Qinghai of song-Nen plain, the Yellow River and Huai He River Haiti district, the Northwest, the Inner Mongol, Shanxi, Hebei and the three provinces in the northeast of China in North China, the saltings that the whole nation has 17 provinces (district) to have area not wait also has 80% left and right sides saline soil not obtain development and use as yet up to now.The alkaline land soil structure is viscid, and air permeability is poor, and the aerobic bacteria reactivity is poor in the soil.The saltings is divided into following three kinds by salinity: slight salinization soil, soil salt content 0.1-0.2%; Moderate salinization soil, soil salt content 0.2-0.4%; Severe salinization soil, soil salt content 0.4-0.6%; (China's great majority calculate saltiness with 30 centimetres of soil plough horizons.) in the main method in existing improvement saltings, the washing salt discharge that physical method adopts causes the disappearance of soil nutrient composition easily, and need bigger material resources and financial resources, and fertilising or Balancing that chemical process adopts cause soil compaction and too high control expense easily.Though the saline and alkaline that the method for physics and chemistry can temporary change soil can not be from changing the soil fertility in original saltings in essence.
Utilize biological modification, particularly little sheath algae technique improvement saltings can increase loose soil property, increases soil permeability, increases soil fertility, repairs problem thereby thoroughly solve salinized soil.
Summary of the invention
One of the object of the invention provides a kind of substratum of culturing little sheath algae;
Two of purpose of the present invention provides acclimation method and the application thereof of a kind of little sheath algae, namely handles the method in saltings with the little sheath algae after the domestication.
As everyone knows, contain 80% the nitrogen of having an appointment in the air, but plant, animal, the mankind and most of microbe all can not directly utilize this gaseous nitrogen as nitrogen nutrition, occurring in nature has only few a part of microorganism nitrogen progressively can be reduced to ammonia and as nitrogenous source, and will be more than by the fixing nitrogen amount height of industry by the fixing nitrogen amount of biological nitrogen fixation.Blue-green algae in the world has 2000 kinds approximately, and wherein the fixed nitrogen blue-green algae is unique biology that can utilize carbonic acid gas and nomadic nitrogen in the photosynthesis fixed air under aerobic conditions.In the fixed nitrogen blue-green algae, little sheath algae (Microcoleus vaginatusSchk.) is the thread kind in the desert algae, little sheath algae plant materials is the filament wall scroll, crawl or form serpentinous curling plant materials, bind each other usually, filament often bunchy exists, there is common glue sheath parcel the outside, the average nitrogen content of cell is 9.7% (w/w), and little sheath algae has the advantages that breeding is fast, photosynthetic efficiency is high, the adverse circumstance adaptive faculty is strong, but most algae can suppress growth even dead when running into strong saline and alkaline.And the present invention tames little sheath algae in specific environment after, make it still can the high-speed rapid growth breeding in the saltings, and the nitrogen, the phosphorus that absorb in a large number in airborne nitrogen and the water be fixed in the frond, finally stays in the saltings.Because the optimal growth water temperature of little sheath algae is 22～28 ℃, temperature be lower than 15 ℃ or be higher than 40 ℃ all unfavorable to growing, the pH value is 7～9, and suitable intensity of illumination is 20000～30000 luxs, is fit to handle the heavy salinized ground of China's Coastal Areas temperature lower (area).
The inventive method is by the preparation substratum, short with growth cycle, the biomass height of accumulation, the wide in range little sheath algae of culture condition is preferred algae kind, it is carried out tolerance of salinity domestication, reduce the nitrogen and phosphorus content domestication and adapt to domestication such as pending water quality, through after the amplification culture step by step, the algae kind is introduced in the saltings, little sheath algae utilizes photosynthesis to breed in a large number, and can absorb, fixedly the nitrogen element in atmosphere and the water source absorbs the phosphoric in the water source, after breed after a while, little sheath algae is cultured water drains, the little sheath algae of spiral shell is deposited in the soil gradually, contains abundant nutritive elements such as nitrogen phosphorus in little sheath algae, after rotten little sheath algae degraded, can improve the nitrogen and phosphorus content in the saltings greatly, the moisture of infiltration can be with saline and alkaline the taking away in the soil, and the little sheath algae improved treatment through repeated multiple times can make soil fertility be enhanced gradually, and can fundamentally solve the problem of salting of soil, finally reach the purpose of improving the ecological environment.
Little sheath algae improvement saltings that utilizes of the present invention, the method for increase alkaline land soil fertility, realize by following steps:
Step 1: screen little sheath algae algae kind, and at the algae kind preparation substratum that filters out;
(1) condition of screening algae kind
Algae kind of the present invention is collected in area, Beijing-Tianjin Ji, North China, after separation and purification voluntarily, chooses well-grown, stabilization characteristics of genetics, and growth cycle is short, the biomass height of accumulation, culture condition is wide in range, and the little sheath algae with fixed nitrogen characteristic is preferred algae kind;
(2) substratum (basic medium) of little sheath algae is cultured in preparation:
Ammonium nitrate 0.5-4.0g/L sal epsom 0.01-0.08g/L
Dipotassium hydrogen phosphate 0.01-0.08g/L calcium chloride 0.01-0.06g/L
Disodium ethylene diamine tetraacetate 0.01-0.3g/L iron(ic) chloride 0.001-0.01g/L
Peptone 0.6-1.5g/L sodium-chlor 1-4g/L
Trace element 0.5-2ml/L
Wherein micro-compound method is:
Boric acid 1.5-4g Manganous chloride tetrahydrate 0.5-3.5g
Zinc sulfate 0.05-0.3g copper sulfate 0.02-0.1g
Xiao Suangu 0.01-0.8g adding distil water is settled to 1L
(3) be 24-26 ℃ in temperature, illumination is the 20000-30000 lux, and pH under 7.0～9.0 the condition, cultivated 6-8 days.
Culture medium prescription is preferably:
Ammonium nitrate 1.0-3.0g/L sal epsom 0.02-0.05g/L
Dipotassium hydrogen phosphate 0.02-0.05g/L calcium chloride 0.015-0.04g/L
Disodium ethylene diamine tetraacetate 0.05-0.2g/L iron(ic) chloride 0.002-0.005g/L
Peptone 0.8-1.2g/L sodium-chlor 2-3g/L
Trace element 0.5-1.5ml/L
Step 2: the purposes by the saltings is carried out the adaptability domestication to little sheath algae:
(1) tolerance of salinity domestication: at the situation on heavy salinized ground, increase the content of sodium-chlor in the basic medium gradually, little sheath algae is adapted to the cultivation of saline and alkaline, can reach the more and more higher salinity of adaptation gradually, and still can keep the high-speed rapid growth breeding, its tolerance of salinity domestication culture medium prescription is:
Ammonium nitrate 1.0-3.0g/L sal epsom 0.02-0.05g/L
Dipotassium hydrogen phosphate 0.02-0.05g/L calcium chloride 0.015-0.04g/L
Disodium ethylene diamine tetraacetate 0.05-0.2g/L iron(ic) chloride 0.002-0.005g/L
Peptone 0.8-1.2g/L trace element 0.5-1.5ml/L
Sodium-chlor is increased to 12g/L gradually from 6g/L
After tolerance of salinity domestication, choose the microscopy activity the algae kind of strong (chromatoplast is more complete, and cellular form is outstanding) do further domestication.
(2). progressively reduce the content of macroelement such as nitrogen, phosphorus in the substratum, make the final concentration of elements such as nitrogen phosphorus be reduced to the concentration of local water quality, trace element does not add.
Sal epsom 0.02-0.05g/L calcium chloride 0.015-0.04g/L
Disodium ethylene diamine tetraacetate 0.05-0.2g/L iron(ic) chloride 0.002-0.005g/L
Peptone 0.8-1.2g/L sodium-chlor 10-12g/L
The 5-2 that ammonium nitrate is reduced to nitrogen content the local water from domestication substratum starting point concentration step by step with reference to the nitrogen content in the local water quality doubly;
The 5-2 that dipotassium hydrogen phosphate is reduced to phosphorus content the local water from domestication substratum starting point concentration step by step with reference to the phosphorus content in the local water quality doubly;
Culture condition through after the domestication of 3-5 growth cycle, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3) will take from local water source through sterile filtration, as the domestication substratum, the nutritive element of substratum is the main nutritive element in local water source, and culture condition is under the physical environment, through after the domestication of 3-5 growth cycle, choose the stronger algae kind of microscopy activity and do further domestication.
(4) with local water source, be left intact, as the domestication substratum, the algal species cultivation condition is under the physical environment, and through the 3-5 growth cycle, the domestication stage finishes.
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1 with algae kind and substratum by volume: 4-1: 6 ratio inoculation, the nutrient solution system is 80-100L, culture condition is: 24～26 ℃ of temperature, illumination 20000-30000 lux, pH is 7.0～9.0, cultivate after 5-9 days in closed reaction vessel, it is 1.5-3.2g/L that the little sheath algae in the nutrient solution reaches dry cell weight.
Step 4: with this algae kind in 1: the ratio of 80-120 is put in the both culturing microalgae pond, and the pond volume is preferably at 15-20m
3, to culture under the open-air atmosphere 15-20 days, little sheath frustule dry weight may ultimately reach 0.5-2.0g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: will build the dam fence around the modified unit, saltings, with (50-100) * (50-100) rice be a processing unit, the preferred 30-40cm of the height of building a dam, utilize conventional irrigation and drainage facility, the water source of locality is introduced in the processing unit in saltings, keeping water surface degree of depth 3-5cm is advisable, be provided with little algae inlet tube in the processing unit of the present invention, in order to the little sheath algae after the enlarged culturing is incorporated into the processing unit of saltings from the pond culture system, little algae inlet tube is advisable apart from floor level 30-50cm, by the flow velocity of little algae inlet tube with 40-60L/min little sheath algae throwing is sprinkling upon in the saltings, generally speaking, each processing unit algae kind inoculum size is 0.04-0.08g/m
2, preferred 0.06-0.08g/m
2Be 0.2-0.8 rice in the distance ground degree of depth, be provided with water table aquifer water shoot (hidden pipe), can be arranged in parallel between the water shoot, also can be crisscross arranged, or arranging all around in processing unit, the water shoot spacing between pipes is 5-7 rice when be arrangeding in parallel, valve is installed on the water shoot, on water shoot, have water port every 1-2 rice, so that can thoroughly drain the moisture of culturing little sheath algae, water shoot is communicated with the waterways of irrigation and drainage system, and the degree of depth in waterways needs the degree of depth greater than water shoot, drainage pipeline can be selected resistance to compression and anti-saline alkali corrosion material, as PVC material etc.;
Because the optimal growth water temperature of little sheath algae is 24～26 ℃, can carry out in spring, summer, autumn so handle the saltings with little sheath algae, as run into the extreme climate influence, can strengthen algae kind input amount or prolong the micro algae growth cycle.
Handle the saltings with the inventive method, the fertility of saltings topsoil is obviously increased, simultaneously, the cultivating microalgae water also can be taken away a large amount of salts in the saltings when discharging, after measured, after once little sheath algae is handled, nitrogen content in the saltings, increased 19-22% than the soil of handling without little sheath algae, phosphorus has increased 13-15%, the total salt quantity 25-30% that descended, and utilize chemical process to handle, obtain identical getting fat effect, need apply phosphogypsum or the urea of 80-100kg in equal area, the present invention can carry out repeatedly little algae and handles according to waiting to plant the needs of plant to soil, to increase the micro algae biomass in the processing unit, improves system of units to the effect of improving in saltings.Handle the saltings with the inventive method, can effectively reduce treatment cost, the improving technology of little algae has not only increased the nitrogen and phosphorus content in the soil, the soil nutritive element of also having avoided the physics WATER-WASHING METHOD to cause is lost, the secondary pollution that can effectively avoid hardening function that the long-term application chemical fertilizer causes the soil again and water source and environment are caused.If life-time service the inventive method improvement saltings can progressively increase soil fertility, improve planting effect.The present invention handles the method in saltings, will make positive contribution for the ecotope of accelerating alkaline land soil and recovering, enlarge the acreage under cultivation of coastland and then improve whole saline and alkaline area.
Description of drawings:
The little sheath algae improvement of Fig. 1 saltings process flow diagram
The growth curve ◇ that the little sheath algae of Fig. 2 is cultivated in different substratum: FN33 substratum; : BG-11 substratum;
The comparison of little sheath algae growing state before and after Fig. 3 tames
Nitrogen content changed before and after Fig. 4 improved heavy salinizedly
Phosphorus content changed before and after Fig. 5 improved heavy salinizedly
Embodiment
Embodiment 1: handle coastal saline and alkaline area, Yingkou, Liaoning
Step 1:
1. prepare the FN33 substratum that the present invention cultures little sheath algae:
Ammonium nitrate 3.0g/L sal epsom 0.04g/L
Dipotassium hydrogen phosphate 0.05g/L calcium chloride 0.015g/L
Disodium ethylene diamine tetraacetate 0.1g/L iron(ic) chloride 0.002g/L
Peptone 1.0g/L sodium-chlor 2.0g/L
Trace element 0.5ml/L
Wherein micro-compound method is:
Boric acid 286mg copper sulfate 5mg
Manganous chloride tetrahydrate 115mg ammonium molybdate 3.7mg
Zinc sulfate 12mg adding distil water is settled to 1L
Culture condition is: 25 ℃ of temperature, and illumination 22000 luxs, pH:8.0 cultivated through 7 days, and the dry cell weight of little sheath algae can reach 1.8g/L, as shown in Figure 2.
2.BG-11 culture medium prescription is as follows:
SODIUMNITRATE 1.0g/L sal epsom 0.075g/L
Dipotassium hydrogen phosphate 0.04g/L calcium chloride 0.036g/L
Disodium ethylene diamine tetraacetate 0.05g/L ironic citrate 0.6g/L
Yellow soda ash 0.02g/L trace element 0.5-1.5ml/L
Culture condition is: 25 ℃ of temperature, and illumination 22000 luxs, pH:8.0 cultivated through 10 days, and the dry cell weight of little sheath algae can reach 0.9g/L, as shown in Figure 2.
Result according to above-mentioned two kinds of little sheath algaes of culture medium culturing can learn, use FN33 substratum provided by the present invention, can make little sheath algae growth cycle shorten 2～4 days than traditional substratum, and the accumulation of its biomass is higher 1～1.5 times than using traditional substratum, has overcome traditional culture medium culturing little sheath algae poor growth and the low shortcoming of biomass.
Step 2: the purposes by the saltings is carried out the adaptability domestication to little sheath algae:
(1). tolerance of salinity domestication: at the situation on heavy salinized ground, little sheath algae is adapted to the cultivation of saline and alkaline, can reach the more and more higher salinity of adaptations gradually, and still can keep the high-speed rapid growth breeding, its tolerance of salinity is tamed culture medium prescription and is:
Ammonium nitrate 3.0g/L sal epsom 0.04g/L
Dipotassium hydrogen phosphate 0.05g/L calcium chloride 0.015g/L
Disodium ethylene diamine tetraacetate 0.1g/L iron(ic) chloride 0.002g/L
Peptone 1.0g/L trace element 0.5ml/L
Sodium-chlor is increased to 10g/L gradually from 6g/L
After tolerance of salinity domestication, the salt tolerance of little sheath algae obviously increases, in containing the domestication substratum of 10g/L sodium-chlor, cultivated 9 days, the algae kind is than the little sheath algae without domestication, dry cell weight has increased about 70% (Fig. 3), choose the algae kind of microscopy activity strong (chromatoplast is more complete, and cellular form is outstanding) and do further domestication.
(2). progressively reduce the content of macroelement such as nitrogen, phosphorus in the substratum, make the final concentration of elements such as nitrogen phosphorus be reduced to the concentration of local water quality:
Sal epsom 0.04g/L calcium chloride 0.015g/L
Disodium ethylene diamine tetraacetate 0.1g/L iron(ic) chloride 0.002g/L
Peptone 1.0g/L sodium-chlor 10g/L
Ammonium nitrate is reduced to 0.02g/L with reference to the nitrogen content in the local water quality step by step from domestication substratum starting point concentration
Dipotassium hydrogen phosphate is reduced to 0.006g/L with reference to the phosphorus content in the local water quality step by step from domestication substratum starting point concentration
Culture condition after the domestication through 4 growth cycles, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3). will take from local water source through sterile filtration, as the domestication substratum, the nutritive element of substratum is the main nutritive element in local water source, and culture condition is under the physical environment, through after the domestication of 3-5 growth cycle, choose the stronger algae kind of microscopy activity and do further domestication.
(4) with local water source, be left intact, as the domestication substratum, the algal species cultivation condition is under the physical environment, and through the 3-5 growth cycle, the domestication stage finishes.
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1: 4 ratio inoculation by volume with algae kind and substratum, the nutrient solution system is 80L, culture condition is: 25 ℃ of temperature, illumination 25000 luxs, pH is 8.0, cultivate after 5 days in closed reaction vessel, the little sheath frustule dry weight in the nutrient solution is 2.6-3.2g/L.
Step 4: this algae kind is put in the both culturing microalgae pond in 1: 120 ratio, and the pond volume is preferably at 20m
3, to culture under the open-air atmosphere 20 days, little sheath frustule dry weight may ultimately reach 0.5-1.8g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: will build the dam fence around the modified unit, saltings, be a processing unit with 100 * 100 meters, the preferred 40cm of the height of building a dam, utilize conventional irrigation and drainage facility, the water source of locality is introduced in the processing unit in saltings, kept water surface degree of depth 4cm and be advisable, the little algae after the enlarged culturing is incorporated into the processing unit of saltings from the pond culture system by little algae inlet tube, little algae flow velocity is 50L/min, and each processing unit algae kind inoculum size is 0.07g/m
2Be 0.8 meter in the distance ground degree of depth, be provided with the water table aquifer water shoot, valve is installed on the water shoot, have water port every 2 meters on water shoot, be arranged in parallel between the water shoot, spacing between pipes is 6 meters.
Because the optimal growth water temperature of little sheath algae is 22～28 ℃, can carry out in spring, summer, autumn so handle the saltings with little sheath algae, as run into the extreme climate influence, can strengthen algae kind input amount or prolong the micro algae growth cycle.
Handle the saltings with the inventive method, the fertility of saltings topsoil is obviously increased, simultaneously, the cultivating microalgae water also can be taken away a large amount of salts in the saltings when discharging, after measured, and after once little algae is handled, nitrogen content in the saltings, increased by 20% than the soil of handling without little algae, phosphorus has increased by 14%, and total salt quantity has descended 27%.(Fig. 4-5)
Handle coastal saline and alkaline area, Yingkou, Liaoning
Step 1: the substratum (basic medium) of little sheath algae is cultured in preparation:
Ammonium nitrate 1.0g/L sal epsom 0.05g/L
Dipotassium hydrogen phosphate 0.02g/L calcium chloride 0.03g/L
Disodium ethylene diamine tetraacetate 0.2g/L iron(ic) chloride 0.003g/L
Peptone 1.2g/L sodium-chlor 3.0g/L
Trace element 1.0ml/L
Culture condition is: 24 ℃ of temperature, illumination 20000 luxs, pH:8.5, growth cycle 8 days.
Step 2: the purposes by the saltings is carried out the adaptability domestication to little sheath algae:
(1) tolerance of salinity domestication:
Ammonium nitrate 1.0g/L sal epsom 0.05g/L
Dipotassium hydrogen phosphate 0.02g/L calcium chloride 0.03g/L
Disodium ethylene diamine tetraacetate 0.2g/L iron(ic) chloride 0.003g/L
Peptone 1.2g/L trace element 1.0ml/L
Sodium-chlor is increased to 11g/L gradually from 6g/L
After tolerance of salinity domestication, choose the microscopy activity the algae kind of strong (chromatoplast is more complete, and cellular form is outstanding) do further domestication.
(2) the content culture medium prescription that progressively reduces macroelement such as nitrogen, phosphorus in the substratum is adjusted into:
Sal epsom 0.05g/L calcium chloride 0.03g/L
Disodium ethylene diamine tetraacetate 0.2g/L iron(ic) chloride 0.003g/L
Peptone 1.2g/L sodium-chlor 11g/L
Ammonium nitrate is reduced to 0.03g/L with reference to the nitrogen content in the local water quality step by step from domestication substratum starting point concentration
Dipotassium hydrogen phosphate is reduced to 0.009g/L with reference to the phosphorus content in the local water quality step by step from domestication substratum starting point concentration
Culture condition after the domestication through 3 growth cycles, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3). will take from local water source through sterile filtration, and further tame as the domestication substratum, method is with embodiment 1;
(4) with local water source, further to tame as the domestication substratum, method is with embodiment 1;
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1: 6 ratio inoculation by volume with algae kind and substratum, the nutrient solution system is 100L, cultivate after 9 days in closed reaction vessel, the little sheath frustule dry weight in the nutrient solution is 1.5-2.8g/L.
Step 4: this algae kind is put in the both culturing microalgae pond in 1: 100 ratio, and the pond volume is preferably at 15m
3, to culture under the open-air atmosphere 15 days, little sheath frustule dry weight may ultimately reach 1.0-1.6g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: with 100 * 100 meters be a processing unit, keep water surface degree of depth 3cm, little sheath algae is introduced processing unit with the flow velocity of 40L/min, each processing unit algae kind inoculum size is 0.06g/m
2, around water shoot is arranged in the processing unit, have water port every 1.5 meters on the water shoot, be 0.3 meter apart from the ground degree of depth.
After step 6, little algae grew through 8 days, when treating that the growth breeding is 1.0g/L to dry cell weight, the valve on the opening water discharge pipe drained residue moisture content, and the little algae improved, process of this round finishes.
After little sheath algae was handled, the nitrogen content in the saltings had increased by 19% than the soil of handling without little algae, and phosphorus has increased by 13%, and total salt quantity has descended 25%.
Embodiment 3:
Handle coastal saline and alkaline area, Yingkou, Liaoning
Step 1: the substratum of little sheath algae is cultured in preparation:
Ammonium nitrate 1.4g/L sal epsom 0.02g/L
Dipotassium hydrogen phosphate 0.03g/L calcium chloride 0.04g/L
Disodium ethylene diamine tetraacetate 0.05g/L iron(ic) chloride 0.005g/L
Peptone 0.8g/L sodium-chlor 3.0g/L
Trace element 1.5ml/L
Culture condition is: 26 ℃ of temperature, illumination 28000 luxs, pH:8.5, growth cycle 8 days.
Step 2: the purposes by the saltings is carried out the adaptability domestication to little sheath algae:
(1). tolerance of salinity domestication:
Ammonium nitrate 1.4g/L sal epsom 0.02g/L
Dipotassium hydrogen phosphate 0.03g/L calcium chloride 0.04g/L
Disodium ethylene diamine tetraacetate 0.05g/L iron(ic) chloride 0.005g/L
Peptone 0.8g/L trace element 1.5ml
Sodium-chlor is increased to 12g/L gradually from 6
After tolerance of salinity domestication, choose the microscopy activity the algae kind of strong (chromatoplast is more complete, and cellular form is outstanding) do further domestication.
(2). the content culture medium prescription that progressively reduces macroelement such as nitrogen, phosphorus in the substratum is adjusted into:
Sal epsom 0.02g/L calcium chloride 0.04g/L
Disodium ethylene diamine tetraacetate 0.05g/L iron(ic) chloride 0.005g/L
Peptone 0.8g/L sodium-chlor 12g/L
Ammonium nitrate is reduced to 0.05g/L with reference to the nitrogen content in the local water quality step by step from domestication substratum starting point concentration
Dipotassium hydrogen phosphate is reduced to 0.015g/L with reference to the phosphorus content in the local water quality step by step from domestication substratum starting point concentration
Culture condition after the domestication through 5 growth cycles, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3). will take from local water source through sterile filtration, and further tame as the domestication substratum, method is with embodiment 1;
(4) with local water source, further to tame as the domestication substratum, method is with embodiment 1;
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1: 5 ratio inoculation by volume with algae kind and substratum, the nutrient solution system is 90L, cultivate after 7 days in closed reaction vessel, the little sheath frustule dry weight in the nutrient solution is 1.8-3.0g/L.
Step 4: this algae kind is put in the both culturing microalgae pond in 1: 80 ratio, and the pond volume is preferably at 18m
3, to culture under the open-air atmosphere 18 days, little sheath frustule dry weight may ultimately reach 1.5-2.0g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: with 100 * 100 meters be a processing unit, keep water surface degree of depth 5cm, little sheath algae is introduced processing unit with the flow velocity of 60L/min, each processing unit algae kind inoculum size is 0.08g/m
2, having water port every 1.5 meters on the water shoot, water shoot is 0.5 meter apart from the ground degree of depth, spacing between pipes is 5 meters,
After step 6, little algae grew through 10 days, the valve on the opening water discharge pipe drained residue moisture content, and the little algae improved, process of this round finishes.
After little sheath algae was handled, the nitrogen content in the saltings had increased by 22% than the soil of handling without little algae, and phosphorus has increased by 15%, and total salt quantity has descended 30%.
Claims (9)
1. the acclimation method for the treatment of little sheath algae in saltings is characterized in that comprising the steps:
(1) screening algae kind;
(2) substratum of little sheath algae is cultured in preparation; The culture medium prescription of the little sheath algae of described breed is:
Wherein micro-compound method is:
Boric acid 1.5-4g Manganous chloride tetrahydrate 0.5-3.5g
Zinc sulfate 0.05-0.3g copper sulfate 0.02-0.1g
Xiao Suangu 0.01-0.8g adding distil water is settled to 1L;
(3) be that temperature 24-26 ℃, illumination are that 20000-30000 lux, pH are under 7.0～9.0 the condition in temperature, cultivated 6-8 days;
(4) increase sodium chloride concentration in the substratum gradually, little sheath algae is carried out tolerance of salinity domestication;
(5) at pending regional water quality situation, progressively reduce the content of nitrogen in the substratum, phosphoric, little sheath algae is adapted to the domestication of nitrogen, phosphorus environment;
(6) with the water source in pending area through sterile filtration, as substratum, the domestication that little sheath algae is conformed;
(7) with local water source, as substratum, under physical environment, little sheath algae is carried out the domestication of growing environment;
(8) get standby to little sheath algae algae kind after the domestication.
2. the acclimation method of little sheath algae as claimed in claim 1 is characterized in that the culture medium prescription of the little sheath algae of described breed is:
3. the acclimation method of little sheath algae as claimed in claim 1, it is characterized in that tolerance of salinity domestication is that the sodium-chlor in the substratum is increased to 12g/L gradually from 6g/L, the domestication that adapts to nitrogen, phosphorus environment is to make 5-2 that the final concentration of nitrogen in the substratum, phosphoric is reduced to nitrogen, phosphorus concentration in the pending regional water quality doubly.
4. the acclimation method of little sheath algae as claimed in claim 1 is characterized in that the described domestication time that little sheath algae is adapted to the domestication of nitrogen phosphorus, the domestication that conforms and growing environment is 3-5 growth cycle.
5. the little sheath algae after taming is according to claim 1 handled the method in saltings, it is characterized in that comprising the steps:
(1) in the laboratory, the little sheath algae algae kind after the domestication and pending regional water source are pressed 1: 4-1: 6 volume ratio inoculation is 24-26 ℃ in temperature, illumination is the 20000-30000 lux, pH is under 7.0～9.0 the condition, cultivates 5-9 days in closed reaction vessel, expands numerous;
(2) will expand algae kind after numerous cultivation in 1: the ratio of 80-120 is put in the both culturing microalgae pond, cultures 15-20 days under the open-air atmosphere;
(3) build the dam fence around in unit, saltings to be improved, the water source of locality is introduced in the processing unit in saltings;
(4) little algae inlet tube is set in processing unit, by the flow velocity of little algae inlet tube with 40-60L/min little sheath algae throwing is sprinkling upon in the saltings;
(5) be 0.2-0.8 rice in the distance ground degree of depth, water shoot is set, valve is installed on the water shoot, water shoot is communicated with the waterways of irrigation and drainage system;
(6) little sheath algae through after the growth breeding in 8-10 days, when the biomass for the treatment of little sheath algae was 1.1-1.6g/L, the valve on the opening water discharge pipe drained residue moisture content, makes little sheath algae be deposited on upper soll layer in the saltings, the little algae improved, process of this round finishes.
6. little sheath algae as claimed in claim 5 is handled the method in saltings, it is characterized in that the height 30-40cm that the saltings processing unit is built a dam, and introduces the depth of water 3-5cm in saltings.
7. little sheath algae as claimed in claim 5 is handled the method in saltings, it is characterized in that each processing unit algae kind inoculum size is 0.04-0.08g/m
2
8. little sheath algae as claimed in claim 5 is handled the method in saltings, it is characterized in that can be arrangeding in parallel between the water shoot, also can be crisscross arranged, or arranging all around in processing unit, have water port every 1-2 rice on the water shoot, spacing between pipes was 5-7 rice when water shoot be arranged in parallel.
9. little sheath algae as claimed in claim 5 is handled the method in saltings, it is characterized in that the material of water shoot selection resistance to compression and anti-saline alkali corrosion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010263525 CN101979496B (en) | 2010-08-26 | 2010-08-26 | Domestication method for microcoleus vaginatus and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010263525 CN101979496B (en) | 2010-08-26 | 2010-08-26 | Domestication method for microcoleus vaginatus and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101979496A CN101979496A (en) | 2011-02-23 |
| CN101979496B true CN101979496B (en) | 2013-09-18 |
Family
ID=43600041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010263525 Expired - Fee Related CN101979496B (en) | 2010-08-26 | 2010-08-26 | Domestication method for microcoleus vaginatus and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101979496B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438008A (en) * | 2022-01-28 | 2022-05-06 | 青岛普瑞邦生物工程有限公司 | Artificial seawater domestication method for protodinoflagellate producing diarrhetic shellfish poison |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867953A (en) * | 2017-03-15 | 2017-06-20 | 哈尔滨工业大学 | A kind of method that microalgae processes molasses containing waste water synchronization production capacity under cryogenic |
| CN107493714A (en) * | 2017-10-09 | 2017-12-22 | 常州明华运输有限公司 | A kind of administering method of salinized soil |
| CN107971334B (en) * | 2017-11-03 | 2020-11-17 | 中国科学院生态环境研究中心 | System for many ponds-algae water circulating irrigation restores polluted soil |
| CN110093294A (en) * | 2019-05-10 | 2019-08-06 | 湖南农业大学 | Utilize the method for ferromanganese ion in Fe-Mn oxidation flora removal acid waste water |
| CN110589978A (en) * | 2019-09-27 | 2019-12-20 | 山东欧卡环保工程有限公司 | Freshwater algae domestication method and process beneficial to freshwater algae for treating sewage |
| CN113604361A (en) * | 2021-07-16 | 2021-11-05 | 武汉理工大学 | Method for culturing modified living microalgae by using high-salinity wastewater and application thereof |
-
2010
- 2010-08-26 CN CN 201010263525 patent/CN101979496B/en not_active Expired - Fee Related
Non-Patent Citations (7)
| Title |
|---|
| P.A. Roger等.free-living blue-green algae in tropical soils.《Microbiology of tropical soils and plant productivity 》.1982,96-97. * |
| Rama Nagina Singh.reclamation of usar lands in india through blue-green alge.《nature》.1950,第165卷325-326. |
| reclamation of usar lands in india through blue-green alge;Rama Nagina Singh;《nature》;19500225;第165卷;325-326 * |
| 一株淡水螺旋藻的盐度驯化及其净化养殖废水作用;胡海燕 等;《渔业现代化》;20090228;第36卷(第1期);1-4 * |
| 固氮蓝藻在盐碱化土地生态修复中应用的研究进展;张巍 等;《土壤》;20080831;第40卷(第4期);510-516 * |
| 张巍 等.固氮蓝藻在盐碱化土地生态修复中应用的研究进展.《土壤》.2008,第40卷(第4期),510-516. |
| 胡海燕 等.一株淡水螺旋藻的盐度驯化及其净化养殖废水作用.《渔业现代化》.2009,第36卷(第1期),1-4. |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438008A (en) * | 2022-01-28 | 2022-05-06 | 青岛普瑞邦生物工程有限公司 | Artificial seawater domestication method for protodinoflagellate producing diarrhetic shellfish poison |
| CN114438008B (en) * | 2022-01-28 | 2023-09-22 | 青岛普瑞邦生物工程有限公司 | Artificial seawater domestication method for protodinoflagellate producing diarrhea shellfish poison |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101979496A (en) | 2011-02-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105838644B (en) | Complex micro organism fungicide and bacterial manure and preparation method thereof and the application in reparation salt affected soil | |
| Rossi et al. | Cyanobacterial inoculation (cyanobacterisation): Perspectives for the development of a standardized multifunctional technology for soil fertilization and desertification reversal | |
| CN101979496B (en) | Domestication method for microcoleus vaginatus and application thereof | |
| CN102010830B (en) | Spirulina acclimatization method and application thereof | |
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN102010829B (en) | Method for domesticating saline land by microalgae | |
| CN106867545A (en) | A kind of saline-alkali soil restoration agent, preparation method and applications from sludge | |
| CN105950502A (en) | Complex endophytic bacterial inoculant and application thereof in phytoremediation of heavy metal contaminated soil | |
| CN102634465B (en) | Acinetobacter with authigenic nitrogen fixation capacity and application thereof | |
| CN106220405A (en) | A kind of activation of microorganism modifying agent for salt-soda soil and preparation method thereof | |
| CN103964968B (en) | A kind of promote vegetation repair organic fertilizer produced from sludge generated and preparation method and application | |
| CN102010828B (en) | Domestication method of nostoc and application thereof | |
| CN111974791B (en) | Ecological energy farm remediation method for multi-scene application of ionic rare earth mining area soil | |
| CN111771670B (en) | Method for improving river sediment into planting soil | |
| CN112705567A (en) | Repairing material, repairing method and application of ionic rare earth tailings area | |
| CN112088607A (en) | Saline-alkali soil improvement method and application thereof | |
| CN102511278A (en) | Desert control technique using special microbial fertilized psammophyte fertilizer | |
| CN105505843B (en) | One plant of Photosynthetic bacterium strain, the Liquid Fertilizer containing the bacterial strain and preparation method, application | |
| CN104496725A (en) | Organic bacterial manure for lead zinc ore contaminated soil remediation and preparation method of organic bacterial manure | |
| CN102010827B (en) | Method for domesticating cylindrospermum and application thereof | |
| CN104478184B (en) | A kind of method of municipal sludge clean utilization | |
| CN102898197A (en) | Technology for producing growth promoting bioorganic fertilizer by using algae mud as additive and product | |
| CN103739333B (en) | Production method and device of three-phase biological bacterium organic fertilizer | |
| CN112854250A (en) | Method for solidifying road slope soil by combining microorganisms and plants | |
| CN107432131A (en) | A kind of saline and alkali land improvement method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130918 Termination date: 20140826 |
|
| EXPY | Termination of patent right or utility model |