WO2018080014A1

WO2018080014A1 - Chamber device for eyeball and respiratory toxicity evaluation for test material

Info

Publication number: WO2018080014A1
Application number: PCT/KR2017/010062
Authority: WO
Inventors: 전기준; 한세현; 윤종상
Original assignee: 인하대학교 산학협력단
Priority date: 2016-10-24
Filing date: 2017-09-13
Publication date: 2018-05-03
Also published as: KR101846369B1

Abstract

The present invention relates to a toxicity evaluation chamber device and, more specifically, to a toxicity evaluation chamber device: capable of separating and accurately testing toxicity evaluations in which a test material is applied to an eyeball or a respiratory organ of an experimental animal; comprising a chamber unit such that a first chamber, to which a test material is supplied, and a second chamber, to which clean air is supplied, are independently sealed from each other by having a barrier placed therebetween; and enabling only one part of an eyeball and a respiratory organ in an animal cage to be exposed to the first chamber, such that the eyeball and the respiratory organ are not influenced by each other because of the test material and the toxicity of the test material can be independently and accurately evaluated.

Description

Chamber device for evaluating eye or respiratory toxicity for test substances

The present invention relates to a toxicity evaluation chamber device, and more particularly, a first chamber in which a test material is supplied to a toxicity evaluation chamber device capable of accurately testing a toxicity test applied to an eyeball or a respiratory organ of a test animal. And the second chamber to which the clean air is supplied form the chamber part so as to be closed independently from each other with the blocking film interposed therebetween, and only one part of the eye or the respirator of the animal cage is exposed to the first chamber so that the eye and the respirator can be The present invention relates to a toxicity evaluation chamber device which is independent of each other and can independently assess the toxicity of a test substance.

There are many chemicals on the planet and more than 5000 chemicals are used in Korea alone. Chemicals are either artificially produced in the industrial world or naturally produced during industrial processes. However, these chemicals are released without being properly measured how they affect the environment, which affects the ecosystem and humans a lot. In order to predict or detect these problems in advance, humans have been evaluating the toxicity of dangerous substances using various toxicity assessment methods. In addition, based on the data measured by the toxicity assessment method, we are trying to minimize the damage to humans and nature caused by toxic substances by giving a regulated amount to dangerous substances.

Conventional toxicity evaluation devices are generally configured to generate test materials in an aerosol state and supply them to a storage chamber of a certain size, and to put the test animals in such storage chambers to be exposed to the test substances and to measure various changes in the test animals. It is. At this time, the experimental animals are kept in a separate breeding cage for a certain period of time and undergoes a process that is sufficiently purified and then proceeds to be put into an inhalation toxicity evaluation test in an optimal health state.

In general, animals such as rats, rabbits, and monkeys, which are used as experimental animals, have an eye and a respiratory tract connected to each other. The test substance is exposed not only to the eye of the experimental animal but also to the respiratory tract because the animal is placed and the test substance is introduced into the laboratory chamber to expose the test substance to the experimental animal. It is difficult to judge correctly.

On the other hand, if the toxic substance to be tested is to be subjected to the toxicological evaluation to the respiratory tract of the test animal, the test animal is placed in the test chamber and the test substance is introduced into the test chamber to expose the test substance to the test animal. The substance is exposed not only to the animal's respiratory system but also to the eye, making it difficult to accurately determine the toxicity of the test substance to the respiratory system of the experimental animal.

SUMMARY OF THE INVENTION The present invention is to solve the problems of the conventional toxicity evaluation chamber apparatus mentioned above, and an object of the present invention is to evaluate the toxicity of a test substance by accurately classifying the toxicity evaluation applied to the eye or respiratory organ of a test animal. It is to provide a chamber device.

Another object of the present invention is to form a chamber in which the first chamber to which test air is supplied and the second chamber to which clean air is supplied are sealed independently of each other with a barrier film interposed therebetween, and one part of the eye or respirator of the animal cage. By only exposing the first chamber to the first chamber, the eye and the respiratory tract are not influenced by the test substance and provide a toxicity evaluation chamber device capable of independently evaluating the toxicity of the test substance.

Another object of the present invention is to form a respiratory opening to which the respirator of the experimental animal is exposed at one end of the animal cage into which the experimental animal is input, and to form an eye opening corresponding to the eye position of the experimental animal to be injected into the animal cage. Toxicity evaluation chamber device which exposes the opening to only one of the first chamber or the second chamber so that the eye and respirator are not influenced by the test substance and can accurately perform the toxicity evaluation of the test substance against the eye or the respirator. To provide.

Another object of the present invention is to form a close contact around the inner circumferential surface of the respirator opening of the animal cage to close the first chamber and the second chamber by closely contacting the experimental animal to the close contact when the experimental animal is introduced into the animal cage It is to provide a toxicity evaluation chamber device that can be sealed.

Another object of the present invention is to form a closed portion protruding from the eye opening of the animal cage to the inner space of the animal cage, when the experimental animal is put into the animal cage, the closed portion and the experimental animal is in close contact with the eye opening and the animal cage To provide a toxic evaluation chamber device that can block the internal space of each other

In order to achieve the object of the present invention, the toxicity evaluation chamber apparatus according to the present invention comprises a double chamber portion having a first chamber and a second chamber, each of which is separated from each other by a barrier film and has an independent sealed internal space; A test supply line communicating with one surface of the first chamber to supply the test material from the outside of the middle chamber part to the internal space of the first chamber, and a separate suction to suck the test material from the internal space of the first chamber A test discharge line communicating with the other surface of the first chamber to be connected to the pump, and an air supply line communicating with one surface of the second chamber to supply clean air from the outside of the double chamber to the internal space of the second chamber. And an animal cage mounted to a through hole formed through the barrier film and capable of feeding and breeding an experimental animal. All.

Preferably, the animal cage according to the present invention is formed to be open so that the cage housing portion is formed in the sealed inner space for the breeding of the experimental animals therein, and the respirator of the experimental animal is inserted into the cage housing And a respirator opening and an eyeball opening that is open to expose the eye of the experimental animal inserted into the cage housing.

Here, the animal cage according to an embodiment of the present invention is characterized in that the respirator of the experimental animal is exposed to the first chamber through the respiratory opening, and is mounted in the through-hole so that the eye of the experimental animal is exposed to the second chamber through the eye opening. It is done.

Here, the animal cage according to another embodiment of the present invention is characterized in that the respirator of the experimental animal is exposed to the second chamber through the respiratory opening, and the eyelet of the experimental animal is exposed to the first chamber through the eye opening is mounted to the through hole. It is done.

Preferably, the eye opening according to the present invention is characterized in that it comprises an eyeball exposure portion exposed to both eyes of the experimental animal, and a cover portion extending from the cage housing between the eyeball exposure portion to cover the separation space between both eyes. do.

Preferably, the animal cage according to the present invention further includes a blocking portion protruding from the eye opening to the internal space of the animal cage to block the eye opening and the internal space of the animal cage when the experimental animal is put into the internal space of the animal cage. Characterized in that.

Preferably, the animal cage according to the present invention is in close contact with the periphery of the laboratory animal in the state in which the experimental animal is put into the interior space of the animal cage along the circumference of the inner circumferential surface of the respiratory exposure to seal the first chamber and the second chamber independently of each other It characterized in that it further comprises a seal formed.

Toxicity evaluation chamber apparatus according to the present invention has a variety of effects as compared to the conventional toxicity evaluation chamber apparatus.

First, the toxicity evaluation chamber apparatus according to the present invention forms a double chamber portion so that the first chamber to be supplied with the test air and the second chamber to which the clean air is supplied are independently of each other by a barrier film, and the eye opening of the animal cage or By allowing only one portion of the respiratory opening to be exposed to the first chamber, the eye and respiratory tract of the experimental animal are not affected by the test substance and can be independently evaluated for toxicity to the test substance.

Secondly, the toxicity evaluation chamber apparatus according to the present invention forms a respiratory opening portion through which the respirator of the experimental animal is exposed at one end of the animal cage into which the experimental animal is input, and forms an eye opening corresponding to the eye position of the experimental animal introduced into the animal cage. By exposing the respiratory opening to only one of the first chamber or the second chamber, the eye and respiratory of the experimental animal are not affected by the test substance and can perform an accurate evaluation of the toxicity of the test substance to the eye or the respirator. Can be.

Third, the toxicity evaluation chamber apparatus according to the present invention forms a close contact around the inner circumferential surface of the respirator opening of the animal cage, the test animal is in close contact with the sealing portion when the test animal is introduced into the animal cage to perfect the first chamber and the second chamber Toxicity assessments can be carried out in a sealed state.

Fourth, the toxicity evaluation chamber apparatus according to the present invention forms a closed portion protruding from the eye opening of the animal cage into the inner space of the animal cage, when the experimental animal is put into the animal cage, the close contact with the experimental animal is in close contact with the eye opening Toxicity assessment can be performed with the internal spaces of the and animal cages isolated from each other.

1 is a view for explaining the toxicity evaluation chamber device 10 according to an embodiment of the present invention.

2 is a view for explaining the toxicity evaluation chamber apparatus according to another embodiment of the present invention.

3 is a view for explaining the animal cage 200 according to an embodiment of the present invention.

4 is a view for explaining an embodiment of the animal cage according to the present invention.

5 is a view of the animal cage mounted on the toxicity evaluation chamber device viewed from the y-axis direction.

Fig. 6 is a view of the animal cage mounted on the toxicity evaluation chamber device as viewed from the z-axis direction.

7 is a view for explaining a state in which the experimental animal 30 is placed in the animal cage 200.

Technical terms used in the present invention are merely used to describe specific embodiments, it should be noted that it is not intended to limit the present invention. In addition, the technical terms used in the present invention should be interpreted as meanings generally understood by those skilled in the art unless the present invention has a special meaning defined in the present invention, and is excessively comprehensive. It should not be interpreted in the sense of or in the sense of being excessively reduced. In addition, when a technical term used in the present invention is an incorrect technical term that does not accurately express the spirit of the present invention, it should be replaced with a technical term that can be properly understood by those skilled in the art.

Also, the singular forms used in the present invention include plural forms unless the context clearly indicates otherwise. In the present invention, terms such as “consisting of” or “comprising” should not be construed as necessarily including all of the various components or steps described in the invention, and some of the components or some of the steps are included. It should be construed that it may not be, or may further include additional components or steps.

In addition, it should be noted that the accompanying drawings are only for easily understanding the spirit of the present invention and should not be construed as limiting the spirit of the present invention by the accompanying drawings.

Hereinafter, the toxicity evaluation chamber apparatus according to the present invention will be described in more detail with reference to the accompanying drawings.

Referring to FIG. 1, the inner space of the double chamber part 110 is an empty space, and the blocking film 150 is disposed in the inner space of the double chamber part 110 so that the inner space of the double chamber part 110 is separated by the blocking film 150. The inner space of the chamber 110 is separated into two spaces. Here, the blocking film 150 may be separated from the inner opposing surface constituting the double chamber portion 110 by the same separation distance to separate the inner spaces of the double chamber portion 110 into spaces having the same volume. Depending on the field of application, the location of the barrier film may be disposed at various locations under conditions that separate the internal space of the double chamber part 110 into two independent spaces, which is within the scope of the present invention.

On one surface of the first chamber 111 and the second chamber 113 formed by the blocking film 150, a supply line for supplying test air including clean air and toxic substances is formed, and the air supply line 131 is provided. One end of is in communication with one surface of the first chamber 111 to supply clean air to the internal space of the first chamber 111, one end of the test supply line 135 is in communication with one surface of the second chamber (113). It is formed to supply test air to the internal space of the second chamber (113). On the other hand, the other end of the air supply line 131 is connected to the clean air generating unit 20, the clean air generating unit 20 generates the clean air to be supplied to the experimental animal and the first chamber through the air supply line 131 The generated clean air is supplied to the internal space of 111. In addition, the other end of the test supply line 135 is connected to the test air generating unit 25, the test air generating unit 25 generates the test air to be supplied to the experimental animal and through the test supply line 135 the second chamber ( The generated test air is supplied to the internal space of 113).

Here, one end of the air discharge line 133 for discharging clean air supplied from the clean air generating unit 20 to the inner space of the first chamber 111 to the outside is formed in communication with the other surface of the first chamber 111. One end of the test discharge line 137 communicating with the other side of the second chamber 113 to discharge the test air supplied from the test air generator 25 into the internal space of the second chamber 113 to the outside is formed. . Preferably, a test air purifier (not shown) for purifying test air discharged from the second chamber 113 is disposed at the other end of the test discharge line 137 or the first chamber (at the other end of the air discharge line 133). A clean air purifier (not shown) for purifying clean air discharged from 111 may be disposed.

A plurality of through holes 151 penetrating the first chamber 111 and the second chamber 113 are formed in the blocking film 150, and each through hole 151 has an animal cage 200 in which experimental animals are fed and bred. ) Is mounted. Preferably, male and male threads are formed on the inner circumferential surface of the through hole 151 and the front outer circumferential surface of the animal cage inserted into the through hole 151 so that the animal cage 200 is fixed to the through hole 115 by screws. Can be configured.

More preferably, the animal cage 200 is a test animal is inserted, the support 170 for more stably supporting the weight of the experimental animal in the animal cage 200 or the weight of the experimental animal moving in the animal cage It may be formed in the interior space of the two chamber 113, the support 170 to the virtual extension line extending from the through hole 115 so that the animal cage 200 can be inserted into the through hole 151 through An arrangement hole 171 is spaced apart from the through hole 151.

The animal cage 200 has an internal space in which the experimental animal can be fed and raised, and the animal cage 200 has a respirator portion of the experimental animal when the experimental animal is placed in the internal space of the animal cage 200. A respirator opening that can be exposed to 111 and an eyeball portion of the experimental animal to which the second chamber 113 can be exposed. Therefore, when the experimental animal is inserted into the animal cage 200 and inserted into the through hole 151, the respirator and the eye of the experimental animal are separately exposed to each other in the first chamber 111 and the second chamber 113, and thus, the first chamber. Clean air may be supplied to the respirator through the 111, and test air may be supplied to the eyes through the second chamber 113, thereby allowing the test substance to reach the eye while the test substance is not exposed to the respirator. The effect can be judged accurately.

Although the above-described toxicity evaluation chamber apparatus has been described as an embodiment for evaluating the toxicity of a test substance applied to the eye of an experimental animal, the animal cage 200 is mounted in the first chamber 111 according to the field to which the present invention is applied. When the respirator of the experimental animal is exposed to the second chamber 113 and the eye of the experimental animal is exposed to the first chamber 111, test air is supplied to the respirator through the second chamber 113 and the first chamber is provided. Clean air may be supplied to the eye through 111, and thus, the effect of the test substance on the respirator may be accurately determined without the test substance being exposed to the eye.

In the toxicity evaluation chamber device illustrated in FIG. 2, the injection pipe 134 directly supplying clean air to the test animal is further formed in the toxicity evaluation chamber device of FIG. 1 described above. The inside of the) is formed with a connection line 132 connecting the air supply line 131 and the air discharge line 133 with each other, the connection line 132 extends to the respirator opening of the animal cage, the respirator of the experimental animal The injection pipe 134 which supplies clean air directly to the furnace is formed. By directly supplying clean air to the experimental animal disposed in the animal cage through the injection tube 134, the experimental animal can maintain the most comfortable state in the animal cage for a long time.

On the other hand, when the test air is exposed to the eye of the test animal, a connection line is formed from the test supply line 135 to the test discharge line 137 and extends from the connection line to the eye exposed part of the test animal. The reason why the test air is not directly exposed to the eye of the test animal is that, in this case, the test air is injected directly into the eye of the test animal, which causes the test animal to be closed and the toxicity of the correct eye cannot be evaluated. Thus, no eye exposure is placed in the imaginary line extending from the test supply line so that the test substance supplied from the test supply line is not directly injected into the eye of the experimental animal, and the test supply line is disposed away from the eye exposure and the test The test air supplied from the supply line 135 is supplied at a constant flow rate and the corresponding flow of test air is discharged through the test discharge line 137 to generate a natural flow of test material.

Referring to Figure 3 in more detail, the animal cage 200 is inserted into the cage housing 210 and the cage housing 210 is formed with a closed inner space in which the experimental animal can be fed breeding therein The respirator opening 230 is formed to be exposed to expose the respiratory part of the test animal, and the eye opening 250 is formed to be open to expose the eye of the test animal inserted into the cage housing 210. .

Guide holes 275 are formed through the cage housing 210 along the length direction, and movement buttons 271 moving along the guide holes 275 are disposed in the guide holes 275. On the other hand, the control button 273 is fastened to the movement button 271 and moves along the guide hole 275 when the movement button 271 moves along the longitudinal direction of the cage housing 210 inner space is formed. The user moves the movement button 271 to pull the control membrane out of the cage housing 210, and after the experiment animal is put into the inner space of the cage housing 210, the movement button 217 again toward the respirator opening 230 While moving to the respirator of the experimental animal is located in the respiratory opening 230, the eye of the experimental animal is adjusted to the position of the experimental animal so that the eye opening 250. Preferably, the movement button 271 is provided with a fixing means for fixing the position of the control membrane 273, through the movement button 271 and the control membrane 273 in the cage housing 210 in the interior space of the experimental animal After setting the position of the, through the fixing means to prevent the experimental animal from moving in the inner space of the cage housing 210.

Here, the outer circumferential surface of the respirator opening 230 is a portion to be mounted to the through hole of the blocking membrane, and a thread is mounted to the through hole of the blocking membrane to the outer circumferential surface thereof, and the control membrane 273 is the cage housing 210. A sealing member for sealing the cage housing 210 is attached to a portion in contact with the inner circumferential surface thereof so that the animal cage 200 communicates with the first chamber 111 only through the respirator opening 230 when the animal cage 200 is mounted on the barrier membrane.

Looking at an example of the animal cage shown in Figure 4 (a), the eye exposed portion 250 is the area of the eye 33 and the adjacent area of the experimental animal when the experimental animal is placed in the cage housing 210 The entire area may be formed so as to penetrate all, that is, the area between the two eyeballs of the experimental animal is also penetrated together with the eyeball area.

On the other hand, looking at another example of the animal cage shown in Figure 4 (b), the eye exposed portion 250 is formed through the area where the first eye of the experimental animal is located when the experimental animal is inserted into the cage housing 210 The first eye exposed portion 253, the second eye exposed portion 254, and the first eye exposed portion 253 and the second eye exposed portion 254 formed through the second eye exposed portion 253. It may be formed with a partial seal 255 extending in the cage housing 210 so that the inter-region is sealed. The first eye exposed portion 253, the second eye exposed portion 254, and the partial closure 255 allow the test substance to be concentrated and exposed only to the eye of the experimental animal, thereby more accurately assessing the toxicity of the eye to the test substance. Can be done.

As shown in FIG. 5 (a), the animal cage 200 into which the experimental animal 30 is inserted is inserted into the insertion hole 151 of the blocking film 150 through the placement hole 171 of the support 170. Is mounted. According to the field to which the present invention is applied, a plurality of insertion holes 151 and corresponding arrangement holes 171 may be formed in the z-axis direction, and thus applied to a plurality of experimental animals through one toxicity evaluation chamber device. The toxicity of the test substance can be evaluated simultaneously.

As shown in FIGS. 5A and 5B, the first chamber in which only the respirator 31 of the experimental animal is supplied with clean air while the experimental animal 30 is inserted into the animal cage 200 is provided. It is arranged to be exposed to.

As shown in FIG. 6 (a), the animal cage 200 into which the experimental animal 30 is inserted is inserted into the insertion hole 151 of the blocking film 150 through the placement hole 171 of the support 170. Is mounted. According to the field to which the present invention is applied, a plurality of insertion holes 151 and corresponding arrangement holes 171 may be formed in the y-axis direction, which is applied to a plurality of experimental animals through one toxicity evaluation chamber device. The toxicity of the test substance can be evaluated simultaneously.

As shown in FIGS. 6A and 6B, the second chamber in which only the eye 33 of the test animal is supplied with test air while the test animal 30 is inserted into the animal cage 200 is provided. To be exposed.

Referring to FIG. 7, the respirator 31 of the experimental animal 30 is exposed to the respirator exposed portion 230 in the cage housing 210 and the eye 33 of the experimental animal 30 is eyeball. After the test animal 30 is moved in close contact with the respirator exposed part 230 in order to be exposed to the exposed part 250, the control film is fixed to prevent the test animal from moving in the cage housing 210.

The respirator adhesion part 270 is formed on the inner circumferential surface of the respirator exposed part 230, so that the test animal 30 is in close contact with the inner space of the cage housing 210 and the inner space of the cage housing 210 is positioned in the first space. It is sealed from the chamber 111, and only the respirator 31 is exposed to the first chamber through the respirator exposure unit 230.

On the other hand, on the inner circumferential surface of the cage housing 210 is formed an eye closed portion 260 which is spaced apart from the eye exposed portion 250 and protruding downward in a position adjacent to the eye exposed portion 250, thereby providing close contact with the respirator. The airtight space is formed through the portion 270 and the eye closure 260 so that the test air of the second chamber is exposed only to the eyes of the experimental animal.

Through the eyeball closure part 260 and the respirator adhesion part 270, the test air may be prevented from being sucked into the respirator of the test animal through the eyeball exposure part 250, and the injection tube may be provided through the respirator adhesion part 270. This prevents the exposure of clean air to the eye when directly supplying clean air to the test animal, thereby accurately performing eye toxicity evaluation on the test substance.

Although the present invention has been described with reference to the embodiments shown in the drawings, this is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent other embodiments are possible. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

Claims

A double chamber part having a first chamber and a second chamber separated from each other by a blocking film to form an independent closed inner space;

A test supply line communicating with one surface of the first chamber to supply a test material from the outside of the double chamber part to the internal space of the first chamber;

A test discharge line communicating with the other surface of the first chamber so as to be connected to a separate suction pump so as to suck the test substance from the internal space of the first chamber;

An air supply line communicating with one surface of the second chamber so as to supply clean air from the outside of the double chamber part to an internal space of the second chamber; And

An animal experiment chamber device, characterized in that it comprises an animal cage mounted to the through-hole formed through the barrier film and capable of feeding breeding experimental animals.
The method of claim 1, wherein the animal cage

A cage housing part in which a sealed inner space in which the experimental animal is fed and bred is formed;

A respirator opening that is open to expose the respirator of the experimental animal inserted into the cage housing; And

An animal laboratory chamber device, characterized in that it comprises an eyeball opening which is open to expose the eyeball of the experimental animal in the cage housing.
3. The animal cage of claim 2 wherein said animal cage

The respirator of the laboratory animal is exposed to the first chamber through the respirator opening;

An animal laboratory chamber apparatus, wherein the eyeball of the laboratory animal is mounted to the through hole through the eyeball opening to be exposed to the second chamber.
3. The animal cage of claim 2 wherein said animal cage

The respirator of the laboratory animal is exposed to the second chamber through the respirator opening;

An animal laboratory chamber apparatus, wherein the eyeball of the laboratory animal is mounted to the through hole through the eyeball opening to be exposed to the first chamber.
The method of claim 3 or 4, wherein the eyeball opening is

An eye exposed part exposing both eyes of the test animal; And

And a cover portion extending from the cage housing between the eye exposed portions to cover the spaced spaces between the two eyeballs.
The method of claim 3 or 4, wherein the animal cage

It is characterized in that it further comprises a blocking portion protruding from the eyeball opening to the inner space of the animal cage to block the eyeball opening and the inner space of the animal cage when the experimental animal is put into the inner space of the animal cage. Chamber apparatus for animal experiments.
3. The animal cage of claim 2 wherein said animal cage

A seal formed along the inner circumferential surface of the respirator exposed portion so as to be in close contact with the respirator of the test animal in a state where the test animal is inserted into the inner space of the animal cage to seal the first chamber and the second chamber independently of each other; Animal laboratory chamber device comprising a.