WO2018077983A1 - Cartouche d'essai de diagnostic de point d'intervention - Google Patents

Cartouche d'essai de diagnostic de point d'intervention Download PDF

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Publication number
WO2018077983A1
WO2018077983A1 PCT/EP2017/077365 EP2017077365W WO2018077983A1 WO 2018077983 A1 WO2018077983 A1 WO 2018077983A1 EP 2017077365 W EP2017077365 W EP 2017077365W WO 2018077983 A1 WO2018077983 A1 WO 2018077983A1
Authority
WO
WIPO (PCT)
Prior art keywords
zone
cartridge
microfluidic system
fluid
reaction chamber
Prior art date
Application number
PCT/EP2017/077365
Other languages
English (en)
Inventor
David Doolan
Donal Cronin
Eoin O'NUALLAIN
Original Assignee
Radisens Diagnostics Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Radisens Diagnostics Limited filed Critical Radisens Diagnostics Limited
Priority to US16/343,176 priority Critical patent/US11420203B2/en
Priority to EP17801350.4A priority patent/EP3532202A1/fr
Priority to JP2019522290A priority patent/JP2019537003A/ja
Publication of WO2018077983A1 publication Critical patent/WO2018077983A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation

Definitions

  • the invention relates to a point-of-care cartridge.
  • the invention relates to a point-of-care diagnostic assay system based on centrifugal microfluidic technology.
  • Point-of-care diagnostic assay systems based on centrifugal microfluidic technology are quite good at performing the necessary integrated sample preparation and assay measurement steps. Such a centrifugal microfluidic platform with optical detection allows for a variety of assay technologies to be implemented in parallel using a single instrument and disposable cartridges
  • point-of-care diagnostic assay systems include US9182384B2 (Roche), US8415140B2 (Panasonic), US8846380 (Infopia), US5591643 (Abaxis), US5409665 (Abaxis).
  • US Patent Publication No. US 2010/074801 describes an analyser comprising a microchip coupled to a motor, where the microchip acquires a liquid sample by means of capillary action.
  • the microchip overcomes the limitation of using capillary action to move a liquid sample by providing a structure which reduces capillary pressure. This is achieved by providing each channel with an adjoining cavity open to atmospheric pressure, which acts so as to prevent an increase in capillary pressure as the fluid length increases.
  • the microchip structure comprises an inlet for collecting a liquid sample, a capillary cavity for holding a predetermined amount of the liquid sample, a single holding chamber having an analytical reagent, a measuring chamber for measuring the mixture of the liquid sample and the reagent, a channel communicating with the holding chamber and the measuring chamber, and a channel connecting the measuring chamber with an atmospheric vent.
  • a liquid sample in the capillary cavity is transferred by centrifugal force into the holding chamber, where it is mixed with the analytical reagent. This mixture is then transferred out of the holding chamber to the inlet of the measuring chamber by capillary force, from where it is transferred into the measuring chamber itself by rotation of the analyser.
  • the microchip structure is configured such that once the holding chamber has delivered the mixture of the single reagent and the liquid sample to the measuring chamber, the mixture cannot be returned to the holding chamber.
  • US Patent Publication No. US 2015/104814 discloses a sample analysis apparatus for whole blood separation. It comprises a rotatable microfluidic apparatus which comprises a sample chamber for accommodating a sample, a channel that provides a path through which the sample flows, and a valve for opening the channel, which is coupled to a valve driver and a control unit.
  • a separation chamber receives a sample flowing from the sample chamber due to centrifugal force, while a collection chamber for collecting target cells is connected to the separation chamber.
  • the apparatus is rotated to separate the sample into a plurality of layers in the separation chamber according to density gradients of materials in the sample, such as for example a DGM layer, an RBC layer, a WBC layer and a plasma layer.
  • the target material located in the lowermost portion of the separation chamber along with the DGM is then transported to the collection chamber for recovery.
  • a microfluidic system comprising:
  • a cartridge coupled to a motor and adapted to move a fluid sample to a plurality of locations on the cartridge, wherein the cartridge is configured to rotate on an inclined plane with respect to a horizontal plane;
  • the cartridge comprises a chevron shaped or substantially V shaped reaction chamber having at least three zones, wherein a first zone is positioned near the apex of the V shaped reaction chamber to define a detection zone, a second zone positioned near a first end of the V shaped reaction chamber and a third zone positioned near a second end of the V shaped reaction chamber, wherein each of the second zone and the third zone comprise a reagent zone; and
  • the motor and a control module is configured to provide a combination of centrifugal force and gravitational force to move said fluid sample between the at least three zones.
  • the first detection zone comprises a cuvette and positioned at the radial extent of the V shaped reaction chamber.
  • the V shaped chamber extends radially inward on two sides to create two zones that can be independently filled with fluid to define the second zone and third zone.
  • the second and/or third zone comprises a reagent storage and/or rehydration zones.
  • the second and/or third zone comprises a region adapted to be optically interrogated.
  • the cartridge is positioned and configured to rotate at a velocity such that a combination of centrifugal force and gravity moves the fluid sample radially outward and inward respectively.
  • the cartridge rotates at a velocity such that the relative centrifugal force (RCF) is greater than gravity, and the fluid sample can be moved radially outward on the cartridge.
  • RCF relative centrifugal force
  • the centrifugal force ensures that no fluid reaches the second zone or third zone.
  • the cartridge is stationary or rotating slowly, gravity will influence the fluid and move the fluid towards the second zone or third zone.
  • the cartridge is rotated or agitated on an inclined plane with respect to a horizontal plane to create a downward slope for the fluid sample to flow under the influence of gravity.
  • the cartridge is further configurable to be agitated to overcome any effects of surface tension that may prevent the fluid from flowing under the influence of gravity.
  • the cartridge rotates on an inclined plane at an angle of ⁇ from the horizontal plane and wherein the angle is between 10° to 60°.
  • a buffer reservoir is positioned close to the centre of rotation of the cartridge and a module configured for applying a sample directly to the cartridge.
  • the dominant force on the fluid sample meniscus is the centrifugal force such that the centrifugal force is parallel to the upper and lower surface of the first detection zone to provide a meniscus evenly on both surfaces.
  • the second zone comprises a dried reagent.
  • the third zone comprises a dried reagent.
  • the dried reagent remains intact until the second or third zones are rehydrated with the fluid sample and a buffer solution.
  • the dried reagent can be spotted in singular or multiple spots in said second and/or third zones.
  • the second or third zone comprises multiple dried reagents.
  • the cuvette comprises a single volume cuvette configured to allow for optical measurement of the buffer solution, the fluid sample and the rehydrated reagents used in each phase of an assay.
  • the system is configured for performing an immunoturbidimetric or an enzyme-based clinical chemistry assay.
  • a microfluidic system comprising: a cartridge coupled to a motor and adapted to move a fluid sample to a plurality of locations on the cartridge;
  • the cartridge comprises a chevron shaped or substantially V shaped reaction chamber having at least three zones, wherein a first zone is positioned near the apex of the V shaped reaction chamber to define a detection zone, a second zone positioned near a first end of the V shaped reaction chamber and a third zone positioned near a second end of the V shaped reaction chamber; and
  • the motor and a control module is configured to provide a combination of centrifugal force and gravitational force to move said fluid sample between the at least three zones.
  • Figure 1 is a flow chart illustrating a number of sequential steps required to transfer a 2-step dried reagent assay onto a self-contained/single- use/disposable point-of-care (POC) cartridge;
  • POC point-of-care
  • Figure 2 shows a cartridge design embodiment to perform the assay sequence according to a first embodiment of the invention
  • Figure 3 illustrates a normal view of the cartridge surface showing reagent rehydration
  • Figure 4 illustrates a chevron shaped or substantially V shaped reaction chamber having at least three zones, according to one embodiment
  • Figure 5 shows a side view of the cartridge mounted on a motor platform during operation
  • Figure 6 Figure 7 and Figure 8 illustrate the benefit of filling the cuvette by centrifugal force
  • Figure 9 shows a cartridge design embodiment to perform the assay sequence according to an embodiment of the invention which uses a second dried reagent spot in the third reagent zone.
  • Figure 1 illustrates a number of sequential steps required to transfer a 2-step dried reagent assay onto a self-contained/single-use/disposable point-of-care (POC) cartridge.
  • This sequence can be applied to immunoturbidimetric and enzyme-based clinical chemistry assays that require two-step addition & rehydration of reagents R1 and R2 to complete a test measurement.
  • a similar test sequence can be used for a 1 step assay where reagents R1 or R2 are used only.
  • the POC cartridge can include a buffer reservoir and will have a means to apply a sample (for example whole blood, plasma, serum) to the cartridge.
  • the cartridge may contain dried, immobilised reagents (R1 and R2) stored in specific locations on the cartridge that can be rehydrated independently. Depending on where the sample is added in the sequence (option (a) or (b) in Figure 1 ), R1 can be rehydrated by either diluted sample (buffer + sample) or buffer only. R2 is then rehydrated by this same fluid volume.
  • FIG 2 shows a cartridge design embodiment to perform the assay sequence illustrated in the flow chart of Figure 1 , according to a first embodiment of the invention.
  • the cartridge design employs a combination of centrifugal and gravitational microfluidics to move fluids to multiple locations on the cartridge.
  • the cartridge 5 includes a buffer reservoir 10 that will sit at or close to the centre of rotation 25.
  • There is also provided a means for applying a sample directly to the cartridge (not shown in Figure 2).
  • the cartridge 5 comprises a chevron shaped or substantially V shaped reaction chamber 15 having at least three zones.
  • a first zone is positioned near the apex of the V shaped reaction chamber to define a detection zone.
  • a second zone is positioned near a first end of the V shaped reaction chamber and a third zone is positioned near a second end of the V shaped reaction chamber.
  • the motor and a control module is configured to provide a combination of centrifugal force and gravitational force to move said fluid sample between the three zones. In operation, centrifugal force is used to control the delivery of a stored buffer from its reservoir 10 and/or subsequent buffer chambers prior to being delivered to the reaction chamber 15.
  • the reaction chamber 15 is sized such that it is much greater than the buffer reaction volume that will be used.
  • the reaction chamber 15 incorporates three distinct zones: A) cuvette detection zone, B) R1 reagent zone and C) R2 reagent zone.
  • the cuvette 45 is located at the radial extent of the reaction chamber 15 (typically close to the cartridge outer diameter 20).
  • the chamber extends radially inward on two sides to create two zones that can be independently filled with fluid for the R1 and R2 reactions. It is beneficial that each zone is sized such that when occupied by buffer they can hold the entire volume within the zone, i.e. the volume of zone A, B or C is equal or greater than the buffer volume and the entire reaction chamber 15 is at a minimum of 3x greater than the buffer volume.
  • centrifugal microfluidics as the primary means of fluid movement. This can limit/restrict the options available to allow a sequential assay to be performed.
  • a combination of centrifugal force and gravity are used to move fluids radially outward and inward respectively.
  • centrifugal forces When the cartridge 5 rotates at velocities where the relative centrifugal force (RCF) is much greater than gravity, centrifugal forces will dominate and fluid can be moved radially outward on the cartridge.
  • RCF relative centrifugal force
  • gravity will still influence the fluid and can be used to move the fluid.
  • the cartridge 5 is rotated on an inclined plane (from the horizontal) such that the cartridge 5 can be positioned statically to create a downward slope for fluid to flow.
  • This method can be employed to move fluids radially inward on the cartridge when it is aligned in particular orientations.
  • the flow of fluid under gravity can also be aided by gentle agitation/shaking to overcome any effects of surface tension that may prevent fluids from flowing.
  • the buffer stored centrally in the buffer chamber is delivered to the reaction chamber 15 (via a capillary valve 30) by centrifugal force.
  • This buffer volume fills the cuvette 45 (Zone A) and a blank measurement of buffer can be performed.
  • the applied sample in the sample chamber 35 is also delivered by centrifugal force (via a capillary valve 40) into the reaction chamber 15 (Zone A) where it is mixed with the buffer.
  • the sample chamber may include additional sample processing steps such as but not limited to plasma separation or whole blood lysis. A sample measurement can be taken at this point in the test sequence if required (may be used as an internal control).
  • the centrifugal force ensures that no fluid reaches Zones B or C and the dried reagents remain intact until R1 and R2 are to be rehydrated.
  • the cartridge 5 is then aligned to allow the fluid within Zone A to flow to Zone B under gravity (aided by gentle agitation if required).
  • the sample and buffer suspension wets reagent R1 and begins rehydrating it.
  • the rehydration continues for a defined period of time until full rehydration has been achieved. This rehydration can be aided by mixing/agitation.
  • centrifugal force is used to move the sample, buffer and R1 suspension back to the cuvette 45 (Zone A) where a calibration measurement can be performed on this suspension.
  • Figure 3 illustrates a normal view of the cartridge surface showing reagent rehydration.
  • the cartridge 5 is then orientated to allow the fluid to flow from the cuvette 45 to Zone C where the R2 reagent(s) are wetted by the buffer, sample and R1 suspension. Again, rehydration continues for a defined period of time until both dried reagents are fully rehydrated. The rehydration can again be aided by mixing agitation on the cartridge 5. Finally, the entire fluid volume is returned to the cuvette 45 (Zone A) where the final reaction can be monitored. It is worth noting that reagents R1 and/or R2 can be spotted in singular or multiple spots.
  • Illustrated in Figure 4 are the radii r1 and r2, the angles ⁇ and ⁇ 2 and the length L.
  • the reagent spot locations are not shown for simplicity.
  • r1 is the radius at which the distal wall of the reaction chamber in Zone B and Zone C is located while r2 is the radius at which the cuvette is centered in Zone A.
  • the length L is the length of distal wall of the reaction chamber.
  • ⁇ is the angle at which the wall is defined from the centerline (created through the center of rotation 25 and the center of the cuvette) and ⁇ 2 is the angle formed between a notional centerline (through the center of rotation) and the distal wall of the reaction chamber at the extent of the chamber.
  • the reaction chamber is designed symmetrically about the centerline which can be advantageous but is not a requirement and can be designed asymmetrically. It is preferred that the length of the chamber wall (L) does not extend beyond a point such that the angle ⁇ 2 is ⁇ 90°. When the angle ⁇ 2 remains >90°, this ensures that the radius r1 ⁇ r2. Under centrifugal force, this allows fluid to return to the cuvette region at r2 since fluid will tend towards the outer radius.
  • Figure 5 shows a side view of the cartridge 5 mounted during operation.
  • the cartridge rotates on an inclined plane at an angle of ⁇ (from horizontal). It is ideal that the inclined angle is between 10° to 60° preferably 30°(provides sufficient gravity and is beneficial for ease of use). Also highlighted are the directions of the centrifugal force and gravity force. The centrifugal force will always be perpendicular to the axis of rotation, i.e. acts in the radial direction (outward) upon rotation.
  • Figure 3 shows the cartridge rotated to align at an angle of 120° from a zero position.
  • the zero position can be the lowest point of the cartridge plane with respect to the center of rotation to enable operation.
  • Zone B can be filled with fluid from Zone A since the cartridge is secured on an inclined plane.
  • the fluid can be returned to Zone A (cuvette) for detection by centrifugal or gravity driven methods.
  • centrifugal force is used to achieve consistent filling of the cuvette.
  • Figure 6, Figure 7 and Figure 8 illustrate the benefit of filling the cuvette by centrifugal force as opposed to gravity.
  • the optical detection path is normal to the cartridge surface and so is aligned perpendicular to the angle at which the cartridge 5 is inclined. It is important that the cuvette is filled entirely and consistently by a column of fluid to ensure that there are no optical irregularities arising from partially or badly filled cuvettes. If the cuvette is filled by gravity, the dominant force on the liquid meniscus is gravity and so the meniscus shape will be uneven and is likely to wet the upper and lower cuvette surfaces to varying levels ( Figure 6). However, when filled by centrifugal force (Figure 7), the dominant force on the liquid meniscus is the centrifugal force.
  • FIG 8 shows the formed meniscus when viewing the cartridge normal to the axis of rotation.
  • the optical path (which may be larger or smaller than shown) can be filled entirely by centrifugal force. Additionally, filling by centrifugal force also ensures that the cuvette is entirely free from air by preventing any trapped air bubbles forming within the optical window.
  • Figure 9 shows a cartridge design embodiment to perform the assay sequence according to an embodiment of the invention which uses a second dried reagent spot in the third reagent zone.
  • the buffer stored centrally in the buffer chamber 10 is delivered to the reaction chamber 15 (via a capillary valve 30) by centrifugal force.
  • This buffer volume fills the cuvette 45 (Zone A) and a blank measurement of buffer can be performed.
  • the applied sample in the sample chamber 35 is also delivered by centrifugal force (via a capillary valve 40) into the reaction chamber 15 (Zone A) where it is mixed with the buffer.
  • a sample measurement can be taken at this point in the test sequence if required (may be used as an internal control).
  • the centrifugal force ensures that no fluid reaches Zones B or C and the dried reagents remain intact until R1 and R2 are to be rehydrated.
  • the cartridge is then aligned to allow the fluid within Zone A to flow to Zone B under gravity (aided by gentle agitation if required).
  • the sample and buffer suspension wets reagent R1 and begins rehydrating it.
  • the rehydration continues for a defined period of time until full rehydration has been achieved. This rehydration can be aided by mixing/agitation.
  • centrifugal force is used to move the sample, buffer and R1 suspension back to the cuvette 45 (Zone A) where a calibration measurement can be performed on this suspension.
  • the cartridge is then orientated to allow the fluid to flow from the cuvette 45 to Zone C where the R2 reagents (split in to reagents R2-A and R2-B) are wetted by the buffer, sample and R1 suspension. Again, rehydration continues for a defined period of time until both dried reagents are fully rehydrated. The rehydration can again be aided by mixing agitation on the cartridge. Finally, the entire fluid volume is returned to the cuvette 45 (Zone A) where the final reaction can be monitored.
  • microfluidic system of the present invention is suitable for performing any type of immunoturbidimetric and enzyme-based clinical chemistry assay.
  • the microfluidic system of the present invention is very flexible, as it can be used to perform an assay that requires the addition and rehydration of a single reagent, as well as to perform an assay that requires the addition and rehydration of multiple reagents. This is due to the fact that the second and/or third reagent zones of the cartridge can each be provided with multiple reagent spots.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un système microfluidique comprenant une cartouche accouplée à un moteur et conçue pour déplacer un échantillon de fluide vers une pluralité d'emplacements sur la cartouche. La cartouche comprend une chambre de réaction en forme de chevron ou sensiblement en forme de V comportant au moins trois zones, une première zone positionnée près du sommet de la chambre de réaction en forme de V pour délimiter une zone de détection, une deuxième zone positionnée à proximité d'une première extrémité de la chambre de réaction en forme de V et une troisième zone positionnée à proximité d'une seconde extrémité de la chambre de réaction en forme de V. Le moteur et un module de commande sont conçus pour fournir une combinaison de force centrifuge et de force gravitationnelle pour déplacer ledit échantillon de fluide entre au moins trois zones.
PCT/EP2017/077365 2016-10-26 2017-10-25 Cartouche d'essai de diagnostic de point d'intervention WO2018077983A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US16/343,176 US11420203B2 (en) 2016-10-26 2017-10-25 Point-of-care diagnostic assay cartridge
EP17801350.4A EP3532202A1 (fr) 2016-10-26 2017-10-25 Cartouche d'essai de diagnostic de point d'intervention
JP2019522290A JP2019537003A (ja) 2016-10-26 2017-10-25 ポイントオブケア診断アッセイカートリッジ

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662413019P 2016-10-26 2016-10-26
US62/413,019 2016-10-26
EP16195853.3 2016-10-26
EP16195853 2016-10-26

Publications (1)

Publication Number Publication Date
WO2018077983A1 true WO2018077983A1 (fr) 2018-05-03

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019207154A1 (fr) * 2018-04-27 2019-10-31 Radisens Diagnostics Ltd. Cartouche de dosage de diagnostic de point d'intervention

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5409665A (en) 1993-09-01 1995-04-25 Abaxis, Inc. Simultaneous cuvette filling with means to isolate cuvettes
US5591643A (en) 1993-09-01 1997-01-07 Abaxis, Inc. Simplified inlet channels
US20100074801A1 (en) 2006-10-31 2010-03-25 Panasonic Corporation Microchip and analyzer using the same
US8415140B2 (en) 2007-10-04 2013-04-09 Panasonic Corporation Analysis device, and analysis apparatus and method using the same
US8846380B2 (en) 2007-10-08 2014-09-30 Infopia Co., Ltd. Reaction cassette for measuring the concentration of glycated hemoglobin and measuring method thereof
US20150104814A1 (en) 2013-10-15 2015-04-16 Samsung Electronics Co., Ltd. Sample analysis methods and apparatuses and dynamic valve operation methods
US9182384B2 (en) 2007-11-08 2015-11-10 Panasonic Healthcare Holdings Co., Ltd. Analyzing device and analyzing method using same

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5409665A (en) 1993-09-01 1995-04-25 Abaxis, Inc. Simultaneous cuvette filling with means to isolate cuvettes
US5591643A (en) 1993-09-01 1997-01-07 Abaxis, Inc. Simplified inlet channels
US20100074801A1 (en) 2006-10-31 2010-03-25 Panasonic Corporation Microchip and analyzer using the same
US8415140B2 (en) 2007-10-04 2013-04-09 Panasonic Corporation Analysis device, and analysis apparatus and method using the same
US8846380B2 (en) 2007-10-08 2014-09-30 Infopia Co., Ltd. Reaction cassette for measuring the concentration of glycated hemoglobin and measuring method thereof
US9182384B2 (en) 2007-11-08 2015-11-10 Panasonic Healthcare Holdings Co., Ltd. Analyzing device and analyzing method using same
US20150104814A1 (en) 2013-10-15 2015-04-16 Samsung Electronics Co., Ltd. Sample analysis methods and apparatuses and dynamic valve operation methods

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019207154A1 (fr) * 2018-04-27 2019-10-31 Radisens Diagnostics Ltd. Cartouche de dosage de diagnostic de point d'intervention

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