WO2018065741A3 - Procede de concentration d'analytes et systeme de concentration et de detection d'analytes - Google Patents

Procede de concentration d'analytes et systeme de concentration et de detection d'analytes Download PDF

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Publication number
WO2018065741A3
WO2018065741A3 PCT/FR2017/052748 FR2017052748W WO2018065741A3 WO 2018065741 A3 WO2018065741 A3 WO 2018065741A3 FR 2017052748 W FR2017052748 W FR 2017052748W WO 2018065741 A3 WO2018065741 A3 WO 2018065741A3
Authority
WO
WIPO (PCT)
Prior art keywords
concentrating
phase
analytes
drop
detecting
Prior art date
Application number
PCT/FR2017/052748
Other languages
English (en)
Other versions
WO2018065741A2 (fr
Inventor
Florent MALLOGGI
Kiarach MESBAH
Sarah BREGANT
Original Assignee
Commissariat À L’Énergie Atomique Et Aux Énergies Alternatives (Cea)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat À L’Énergie Atomique Et Aux Énergies Alternatives (Cea) filed Critical Commissariat À L’Énergie Atomique Et Aux Énergies Alternatives (Cea)
Priority to US16/339,887 priority Critical patent/US11402307B2/en
Priority to EP17792118.6A priority patent/EP3523623A2/fr
Publication of WO2018065741A2 publication Critical patent/WO2018065741A2/fr
Publication of WO2018065741A3 publication Critical patent/WO2018065741A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4022—Concentrating samples by thermal techniques; Phase changes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/34—Dynamic spectrometers
    • H01J49/40—Time-of-flight spectrometers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0678—Facilitating or initiating evaporation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4022—Concentrating samples by thermal techniques; Phase changes
    • G01N2001/4027—Concentrating samples by thermal techniques; Phase changes evaporation leaving a concentrated sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid

Abstract

La présente invention concerne un procédé de concentration d'au moins un analyte (11) comprenant les étapes suivantes : préparation d'une première phase (1) comprenant au moins un analyte (11); dépôt d'une goutte de ladite première phase (1) sur un substrat (2); dépôt sur ladite goutte de première phase (1) d'une goutte d'une seconde phase (4) liquide comprenant au moins un tensioactif (41), ladite seconde phase (4) étant non miscible avec ladite première phase (1); évaporation de la goutte de la seconde phase (4); et évaporation de la goutte de la première phase (1). La présente invention concerne également un procédé de détection d'au moins un analyte (11) mettant en œuvre ledit procédé de concentration; et un système mettant en œuvre ledit procédé de détection.
PCT/FR2017/052748 2016-10-07 2017-10-06 Procédé de concentration d'analytes WO2018065741A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US16/339,887 US11402307B2 (en) 2016-10-07 2017-10-06 Method for concentrating analytes
EP17792118.6A EP3523623A2 (fr) 2016-10-07 2017-10-06 Procede de concentration d'analytes et systeme de concentration et de detection d'analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1659705 2016-10-07
FR1659705A FR3057354A1 (fr) 2016-10-07 2016-10-07 Procede de concentration d’analytes

Publications (2)

Publication Number Publication Date
WO2018065741A2 WO2018065741A2 (fr) 2018-04-12
WO2018065741A3 true WO2018065741A3 (fr) 2018-08-02

Family

ID=57396729

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2017/052748 WO2018065741A2 (fr) 2016-10-07 2017-10-06 Procédé de concentration d'analytes

Country Status (4)

Country Link
US (1) US11402307B2 (fr)
EP (1) EP3523623A2 (fr)
FR (1) FR3057354A1 (fr)
WO (1) WO2018065741A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115121304B (zh) * 2022-07-08 2023-11-14 厦门大学 一种高通量的颗粒操控系统及检测方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030213905A1 (en) * 2002-05-16 2003-11-20 John Lennon Method for microdispensing of fluids from a pipette
EP1582855A2 (fr) * 2004-03-31 2005-10-05 Horiba, Ltd. Méthode de concentration d'un échantillon liquide, outil de support pour cette concentration et méthode d'analyse de traces d'éléments utilisant cet outil.
US20070039866A1 (en) * 2005-08-22 2007-02-22 Schroeder Benjamin G Device, system, and method for depositing processed immiscible-fluid-discrete-volumes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2388568A1 (fr) 2010-05-17 2011-11-23 Universiteit Twente Procédé de traitement d'une goutte de liquide
US9997344B2 (en) * 2013-05-31 2018-06-12 University Of Washington Through Its Center For Commercialization Methods and devices for generating double emulsions
US10101323B2 (en) 2013-10-23 2018-10-16 Vanderbilt University Liquid diagnostic assays utilizing marangoni flow

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030213905A1 (en) * 2002-05-16 2003-11-20 John Lennon Method for microdispensing of fluids from a pipette
EP1582855A2 (fr) * 2004-03-31 2005-10-05 Horiba, Ltd. Méthode de concentration d'un échantillon liquide, outil de support pour cette concentration et méthode d'analyse de traces d'éléments utilisant cet outil.
US20070039866A1 (en) * 2005-08-22 2007-02-22 Schroeder Benjamin G Device, system, and method for depositing processed immiscible-fluid-discrete-volumes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BALAMURUGAN SUBRAMANIAN ET AL: "Surface Modification of Droplet Polymeric Microfluidic Devices for the Stable and Continuous Generation of Aqueous Droplets", LANGMUIR, vol. 27, no. 12, 21 June 2011 (2011-06-21), US, pages 7949 - 7957, XP055386441, ISSN: 0743-7463, DOI: 10.1021/la200298n *
JEAN-CHRISTOPHE BARET: "Surfactants in droplet-based microfluidics", LAB ON A CHIP, vol. 12, no. 3, 20 October 2011 (2011-10-20), pages 422 - 433, XP055386440, ISSN: 1473-0197, DOI: 10.1039/C1LC20582J *
LINAS MAZUTIS ET AL: "Single-cell analysis and sorting using droplet-based microfluidics", NATURE PROTOCOLS, vol. 8, no. 5, 4 April 2013 (2013-04-04), GB, pages 870 - 891, XP055240820, ISSN: 1754-2189, DOI: 10.1038/nprot.2013.046 *
PHILIPP GRUNER ET AL: "Stabilisers for water-in-fluorinated-oil dispersions: Key properties for microfluidic applications", CURRENT OPINION IN COLLOID AND INTERFACE SCIENCE, vol. 20, no. 3, 30 July 2015 (2015-07-30), GB, pages 183 - 191, XP055386575, ISSN: 1359-0294, DOI: 10.1016/j.cocis.2015.07.005 *
SIMON K KÜSTER ET AL: "Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry: label-free content analysis of single droplets", ANALYTICAL CHEMISTRY, 5 January 2013 (2013-01-05), United States, pages 1285, XP055386567, Retrieved from the Internet <URL:http://pubs.acs.org/doi/pdf/10.1021/ac3033189> [retrieved on 20170630], DOI: 10.1021/ac3033189 *
VAN NGUYEN TRUSKETT ET AL: "Influence of Surfactants on an Evaporating Drop:? Fluorescence Images and Particle Deposition Patterns", LANGMUIR, vol. 19, no. 20, 3 September 2003 (2003-09-03), US, pages 8271 - 8279, XP055386563, ISSN: 0743-7463, DOI: 10.1021/la030049t *

Also Published As

Publication number Publication date
WO2018065741A2 (fr) 2018-04-12
US20200041393A1 (en) 2020-02-06
FR3057354A1 (fr) 2018-04-13
US11402307B2 (en) 2022-08-02
EP3523623A2 (fr) 2019-08-14

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