WO2018056574A1 - Procédé de dépistage de tumeur pulmonaire au moyen de rayons infrarouges proches - Google Patents

Procédé de dépistage de tumeur pulmonaire au moyen de rayons infrarouges proches Download PDF

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Publication number
WO2018056574A1
WO2018056574A1 PCT/KR2017/008771 KR2017008771W WO2018056574A1 WO 2018056574 A1 WO2018056574 A1 WO 2018056574A1 KR 2017008771 W KR2017008771 W KR 2017008771W WO 2018056574 A1 WO2018056574 A1 WO 2018056574A1
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Prior art keywords
infrared
chest wall
tumor
infrared rays
screening method
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PCT/KR2017/008771
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English (en)
Korean (ko)
Inventor
안예찬
옥철호
박은기
김성원
이해영
강현욱
유준식
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부경대학교 산학협력단
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Publication of WO2018056574A1 publication Critical patent/WO2018056574A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57423—Specifically defined cancers of lung

Definitions

  • the present invention relates to a lung tumor screening method using near-infrared rays, and more particularly, near-infrared rays or a plurality of near-infrared rays irradiated to a chest wall tumor are projected through a chest wall through the body in vitro, and then near-infrared rays projected from the chest wall tumor To detect the presence or spread of chest wall tumors by detecting with the camera and imaging with shade, or by detecting with camera with the fluorescent dye injected through blood vessels, or by using the spectrophotometer through the difference in absorbance of multiple infrared near-infrared rays.
  • the present invention relates to a lung tumor screening method using near infrared rays.
  • Asbestos classified as a class 1 carcinogen by the World Health Organization's International Cancer Research Institute (WHO / IARC), is one of the leading causes of malignant mesothelioma, a lung tumor occurring in the pleura. Have a 15 to 40 year incubation period
  • the pleura is composed of the parietal pleura and the lateral pleura (visceral pleura), the pleural plaques that occur in the pleura are also generated by asbestos exposure.
  • these pleural plaques are also caused by tuberculosis, there is a lack of evidence for asbestos-related compensation because the high prevalence of tuberculosis and the lack of evidence for clinical symptoms of the pleural plaque have not been identified.
  • malignant mesothelioma has a poor prognosis (less than 1 year of survival after diagnosis) in terms of treatment, and the incidence and mortality rate are continuously increasing. This is because early diagnosis is very difficult.
  • X-ray chest examination is usually confirmed when the size of the cancer is 5 ⁇ 10mm or more to identify the abnormal area, protected by the ribs and because of the nature of the lungs overlapping with the location of the heart, it is blocked by other organs to identify the abnormal site
  • lung tissue is in direct contact with air, and is closely connected to blood vessels, which is highly likely to be malignant. Therefore, X-ray chest examination has a very low utility as an early diagnosis of lung tumors.
  • Korean Patent Publication No. 10-2005-0113442 Korean Registered Patent Publication No. 10-0266831 and the like can be referred to.
  • the present invention has been made to improve the above-mentioned problems, the object of the present invention is to detect the near-infrared rays irradiated to the position of the chest wall tumor of the pleura through the chest wall from the body through the projection, and then detect them and image them with shadows.
  • the present invention provides a lung tumor screening method using near-infrared rays to confirm the presence and spread of chest wall tumors.
  • Another object is to inject fluorescent dyes into blood vessels and deposit them on chest wall tumors, by detecting near-infrared rays irradiated to the chest wall tumor location of the pleura as the fluorescent dyes emit fluorescence in the chest wall tumors, thereby imaging the neovascularization of the chest wall tumors.
  • the present invention provides a lung tumor screening method using near infrared rays to confirm the presence or absence of a chest wall tumor.
  • Another objective is to measure or image the light intensity of multiple near infrared rays at different wavelengths irradiated simultaneously or sequentially to the chest wall tumor location of the pleura, and to determine the presence of chest wall tumors through differences in absorbance. It provides a method for tumor screening.
  • Pulmonary tumor screening method using the near-infrared of the present invention to achieve the object as described above, the near-infrared irradiated to the position of the chest wall tumor invading the body through the chest wall through the body, and detects the chest wall Imaging the shadow of the tumor.
  • the near-infrared ray is irradiated through the optical fiber inserted to the position of the chest wall tumor in the step of incidence of near-infrared radiation, and the step of detecting the near-infrared ray that is incident on the projection is preferably detected by a near-infrared detection camera.
  • the method may further include inserting the guide sheath into the working channel of the bronchoscope to the position of the chest wall tumor after the insertion of the optical fiber and before the step of irradiating the near infrared rays, thereby inserting the optical fiber into the guide sheath.
  • the said near infrared rays are near infrared rays in the 780-2000 nm wavelength range.
  • the step of injecting a fluorescent dye into the blood vessel before the step of irradiating the near infrared the fluorescent dye is leaked from the immature blood vessels around the chest wall tumor and deposited on the chest wall tumor, the fluorescent by the irradiation of the near infrared
  • Another feature is the imaging of neovascularization of the chest wall tumor that emits light.
  • the fluorescent dye is preferably indocyanine green (Indocyanine green (ICG)).
  • a radiation filter disposed in front of the near-infrared detection camera.
  • an excitation filter disposed in front of the near infrared light source.
  • the near-infrared ray is irradiated with near-infrared rays of different wavelengths, by measuring or imaging the intensity of light of each near-infrared ray passing through the chest wall to determine the presence or absence of chest wall tumors through the difference in absorption have.
  • the presence or absence of the chest wall tumor through the difference in absorbance is performed by a spectroscope or a hyperspectral camera.
  • the lung tumor screening method using the near-infrared ray of the present invention it is necessary to regularly screen for a high-risk group with asbestos exposure in order to diagnose malignant carcinoma as a chest wall tumor, and malignant mesothelioma according to the present invention. Early diagnosis of the disease is effective.
  • FIG. 1 is an anatomical image showing a pleura consisting of a parietal pleura and a lateral pleura
  • Figure 2 shows normal results in PET / CT, but PET / CT and endoscopy (Roca E, Laroumagne S, Vandemoortele T, Berdah S, Dutau H, Maldonado F) showing the diagnosis of malignant mesothelioma using thoracoscopy.
  • Astoul P. "18F-fluoro-2-deoxy-d-glucose positron emission tomography / computed tomography fused imaging in malignant mesothelioma patients: looking from outside is not enough"
  • FIG. 3 is a conceptual diagram showing a lung tumor screening method using near infrared rays according to a first embodiment of the present invention
  • FIG. 4 is an enlarged view of a main part of FIG. 3;
  • FIG. 5 is a conceptual diagram showing a lung tumor screening method using near infrared rays according to a second embodiment of the present invention.
  • FIG. 6 is a conceptual diagram showing a lung tumor screening method using near infrared rays according to a third embodiment of the present invention
  • Figure 7 is an example of applying the lung tumor screening method using the near infrared ray in accordance with the present invention in the animal model
  • Figure 8 is an image showing an example showing a normal chest wall by applying the lung tumor screening method using the near infrared ray in accordance with the present invention in an animal model
  • Figure 9 is an image showing an example showing a chest tumor by applying the lung tumor screening method using the near infrared ray in accordance with the present invention in an animal model
  • FIG. 3 is a conceptual diagram illustrating a lung tumor screening method using near infrared rays according to a first embodiment of the present invention
  • FIG. 4 is an enlarged view of a main part of FIG. 3.
  • Lung tumor screening method using near infrared rays according to the first embodiment of the present invention, a method for obtaining an image of the chest wall tumor using a projection using near infrared rays in order to screen and diagnose the chest wall tumor.
  • Near-infrared radiation is an electromagnetic wave with a wavelength in the range of 780-2000 nm, and is widely used for biodiagnosis due to its high transmittance in tissue.
  • NIR source near-infrared
  • the near-infrared ray passing through the chest wall is detected and imaged by a near-infrared detection camera placed outside the body.
  • the shadow of the chest wall tumor is acquired by the near infrared detection camera in the image of the near infrared ray detection, so that the presence or absence and spread of the chest wall tumor can be known.
  • the near-infrared ray is irradiated to the location of the chest wall tumor by the optical fiber, and as shown in FIG. 4, the projection of the portion having the chest wall tumor by inserting a guide sheath into the working channel of the endoscope.
  • the optical fiber is inserted into the guide sheath to irradiate near-infrared rays to the position of the chest wall tumor through the optical fiber, and to enter the body through the chest wall and enter the projection.
  • the near-infrared rays passing through the chest wall confirm the presence and spread of the chest wall tumor as described above.
  • FIG. 5 is a conceptual diagram showing a lung tumor screening method using near infrared rays according to a second embodiment of the present invention.
  • Lung tumor screening method using a near infrared ray according to a second embodiment of the present invention, a method using a fluorescent imaging using indocyanine green (ICG), a fluorescent dye that emits fluorescence by near infrared rays.
  • ICG indocyanine green
  • ICG Indocyanine green
  • ICG is commonly used for vascular imaging by easily binding to plasma proteins, and has the property of maximizing absorption of 780 nm light to emit 820 nm fluorescence.
  • an emission filter of 820 nm is disposed in front of the near-infrared detection camera, and an excitation filter of 780 nm is disposed in front of the near-infrared light source.
  • the near-infrared irradiation is performed by the optical fiber as described above, and the optical fiber is inserted into the guide sheath of the working channel to position the chest wall tumor as described above.
  • FIG. 6 is a conceptual diagram showing a lung tumor screening method using near infrared rays according to a third embodiment of the present invention.
  • the lung tumor screening method using the near infrared rays simultaneously or sequentially records the multi-wavelength NIR sources of two different wavelengths or multiple wavelengths in the near infrared band.
  • a near-infrared spectroscopy method for determining the presence or absence of a tumor through the difference in absorbance by measuring or imaging the intensity of each near-infrared light passing through the chest wall from the body in vitro is presented.
  • the difference in absorbance is measured by measuring or imaging the near-infrared light intensity by a near-infrared detector such as a spectroscope or a hyperspectral camera located outside the body.
  • FIG. 7 is an image showing an example of applying a lung tumor screening method using a near infrared ray to an animal model according to the present invention by injecting near infrared rays through the chest wall into the body in an endoscopic method.
  • the chest wall near-infrared projection method was performed using the lung tumor screening method using the near-infrared ray of Example 1, using a normal New Zealand white rabbit as an animal model.
  • this image it can be seen that a considerable amount of near-infrared rays are projected to the outside through the chest wall in the areas that appear red.
  • FIG. 8 is an image showing near infrared rays projected through the normal chest wall of New Zealand white rabbit
  • FIG. 9 is an image showing near infrared rays projected through the tumor chest wall.
  • the normal chest wall projection image is shaded parallel to the sternum but the tumor chest wall projection image can be seen that the darker as the shadow of the tumor is added.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
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Abstract

La présente invention concerne un procédé de dépistage de tumeur pulmonaire au moyen de rayons infrarouges proches, le procédé étant mis en œuvre par les étapes consistant à : projeter des rayons infrarouges proches de telle sorte que les rayons infrarouges proches incidents même sur la position d'une tumeur de la paroi thoracique aillent de l'intérieur du corps vers l'extérieur à travers la paroi thoracique ; et détecter les rayons infrarouges proches pour obtenir une image d'une ombre de la tumeur de la paroi thoracique. Pour un diagnostic précoce de mésothéliome malin en tant que tumeur de la paroi thoracique, un dépistage régulier doit être effectué sur un groupe à haut risque avec une histoire d'exposition à l'amiante, et le procédé de diagnostic de la présente invention a pour effet de permettre un diagnostic précoce du mésothéliome malin.
PCT/KR2017/008771 2016-09-26 2017-08-11 Procédé de dépistage de tumeur pulmonaire au moyen de rayons infrarouges proches WO2018056574A1 (fr)

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KR10-2016-0123392 2016-09-26
KR1020160123392A KR101855395B1 (ko) 2016-09-26 2016-09-26 근적외선을 이용한 폐종양 스크리닝 방법

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100340290B1 (ko) * 1994-12-07 2002-06-15 인스티튜트 퓌르 디아그노스틱포르슝 게엠베하 안 더 프라이엔유니베르시태트 베를린 근적외선 조사에 의한 생체내 진단방법
US20080218732A1 (en) * 2005-07-27 2008-09-11 University Of Massachusetts Lowell Infrared Scanner for Biological Applications
JP2013514156A (ja) * 2009-12-15 2013-04-25 エモリー ユニバーシティ 診断又は治療処置においてリアルタイム解剖学的指針を提供するシステム及び方法
US20130211246A1 (en) * 2011-12-27 2013-08-15 Vinod PARASHER METHODS AND DEVICES FOR GASTROINTESTINAL SURGICAL PROCEDURES USING NEAR INFRARED (nIR) IMAGING TECHNIQUES
US20140187967A1 (en) * 2012-12-05 2014-07-03 Fred Wood System and Method for Multi-Color Laser Imaging and Ablation of Cancer Cells Using Fluorescence

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101460908B1 (ko) 2013-08-09 2014-11-17 서울여자대학교 산학협력단 4차원 컴퓨터 단층촬영 영상의 폐종양 위치 추적 시스템 및 그 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100340290B1 (ko) * 1994-12-07 2002-06-15 인스티튜트 퓌르 디아그노스틱포르슝 게엠베하 안 더 프라이엔유니베르시태트 베를린 근적외선 조사에 의한 생체내 진단방법
US20080218732A1 (en) * 2005-07-27 2008-09-11 University Of Massachusetts Lowell Infrared Scanner for Biological Applications
JP2013514156A (ja) * 2009-12-15 2013-04-25 エモリー ユニバーシティ 診断又は治療処置においてリアルタイム解剖学的指針を提供するシステム及び方法
US20130211246A1 (en) * 2011-12-27 2013-08-15 Vinod PARASHER METHODS AND DEVICES FOR GASTROINTESTINAL SURGICAL PROCEDURES USING NEAR INFRARED (nIR) IMAGING TECHNIQUES
US20140187967A1 (en) * 2012-12-05 2014-07-03 Fred Wood System and Method for Multi-Color Laser Imaging and Ablation of Cancer Cells Using Fluorescence

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KR20180033878A (ko) 2018-04-04

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