WO2018049662A1 - Substrat de culture et procédé de culture pour flammulina velutipes - Google Patents

Substrat de culture et procédé de culture pour flammulina velutipes Download PDF

Info

Publication number
WO2018049662A1
WO2018049662A1 PCT/CN2016/099274 CN2016099274W WO2018049662A1 WO 2018049662 A1 WO2018049662 A1 WO 2018049662A1 CN 2016099274 W CN2016099274 W CN 2016099274W WO 2018049662 A1 WO2018049662 A1 WO 2018049662A1
Authority
WO
WIPO (PCT)
Prior art keywords
flammulina velutipes
cultivation
dry
substrate
bottle
Prior art date
Application number
PCT/CN2016/099274
Other languages
English (en)
Chinese (zh)
Inventor
王亮
Original Assignee
王亮
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 王亮 filed Critical 王亮
Priority to PCT/CN2016/099274 priority Critical patent/WO2018049662A1/fr
Publication of WO2018049662A1 publication Critical patent/WO2018049662A1/fr

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the invention relates to a fungus planting substrate, in particular to a Flammulina velutipes cultivation substrate and a cultivation method thereof.
  • Soilless culture A method of cultivating plants with other things, including hydroponics, fog (gas) culture, and substrate cultivation.
  • a soilless culture method in which a plant substrate is fixed with a solid substrate (medium) and the nutrient solution and oxygen are absorbed through the substrate.
  • the commonly used inorganic substrates are vermiculite, perlite, rock wool, sand, polyurethane, etc.; organic substrates include peat, rice husk charcoal and bark. Therefore, substrate cultivation is divided into rock wool cultivation, sand culture, etc., and our agricultural field is also actively conducting research experiments on soilless substrates.
  • the matrix is the key to ensure the quality of the strain.
  • the high-quality matrix can provide a good ventilation environment and suitable water holding capacity for the strain, and has the characteristics of light weight, convenient transportation, no pests and diseases, and economic and environmental protection of raw materials.
  • the quality of the substrate directly affects the growth of planting plants and strains. This has become the focus of continuous research and testing in the field of agricultural matrix research, and the agricultural sector is directly facing the majority of farmers, so it is necessary to consider the cost. How to match a matrix suitable for Flammulina velutipes cultivation with low cost, good yield and good quality is the focus of our research.
  • the object of the present invention is to provide a Flammulina velutipes cultivation substrate and a cultivation method thereof, and to obtain a suitable Flammulina velutipes strain by considering the cost of the matrix raw material, the matrix nutrient and the ratio of the substrate to the fungus planting.
  • the substrate is planted, and a Flammulina velutipes cultivation substrate and a cultivation method thereof are provided.
  • the technical scheme of the invention adopts wheat bran, animal manure, grass ash, dry awning, peat soil and corn cob as matrix material
  • peat soil is a matrix material rich in organic matter
  • wheat bran, animal manure, grass ash, dry awning Corn cobs, matrix materials are easy to obtain and environmentally friendly for farmers.
  • the technical solution of the present invention provides a Flammulina velutipes cultivation substrate, and the matrix raw material comprises the following ratios by volume ratio: 5%-20% of wheat bran, 5%-20% of animal feces, 10%-30% of grass ash, 10% of dried awning -15%, peat soil 15%-35%, corn cob 5%-15%; the substrate pH 5.8 ⁇ 8.0, conductivity 550 ⁇ 750 ⁇ s / cm, water holding capacity 55% ⁇ 70%, air porosity It is 10% to 30%, the total porosity is 50% to 85%, and the bulk density is 0.15 to 0.7 g/cm 3 .
  • the Flammulina velutipes cultivation substrate raw material also includes the following ratios by volume ratio: wheat bran 18%, animal feces 15%, grass ash 27%, dry awning 10%, peat soil 22%, corn cob 6%.
  • the Flammulina velutipes cultivation substrate raw material also includes the following ratios by volume ratio: 6% wheat bran, 11% animal feces, and vegetation Gray 33%, dry awning 15%, peat soil 21%, corn cob 14%.
  • the Flammulina velutipes cultivation substrate raw material also includes the following ratios by volume ratio: wheat bran 15%, animal feces 20%, grass ash 15%, dry awning 14%, peat soil 23%, corn cob 13%.
  • the Flammulina velutipes cultivation substrate raw material also includes the following ratios by volume ratio: wheat bran 20%, animal feces 12%, grass ash 21%, dry awning 10%, peat soil 28%, corn cob 9%.
  • any of the above-mentioned Flammulina velutipes cultivation substrates having a pH of 6.0 to 7.5, an electric conductivity of 650 to 700 ⁇ s/cm, a water holding capacity of 64% to 67%, an air porosity of 21% to 28%, and a total porosity of 55% to 70%, and the bulk density is 0.25 to 0.50 g/cm 3 .
  • the animal manure comprises one or more components of dry chicken manure, dry sheep manure, dry cow dung, dry pig manure, and dry horse manure, which are specifically adjusted according to the supply requirements of the raw materials in the geographical area.
  • the invention also discloses a cultivation method of Flammulina velutipes cultivation substrate,
  • Formulating the substrate mixing the matrix materials according to any one of the above items uniformly, adding water and mixing well, holding water amount of 64% to 70%, and then sterilizing and sterilizing in a jar or a plastic bag;
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the bacteria stage, and find that the infection of the bacteria should be treated or removed in time to prevent spread and spread;
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag. The black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the mushroom of the small cap. ;
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • the water has a pH of 5.0 to 7.4 and an electrical conductivity of 100 ⁇ s/cm to 150 ⁇ s/cm.
  • the substrate cultivation method comprises mushroom stick cultivation.
  • the invention has the beneficial effects that the field of use of the substrate is mostly in the agricultural field, and the matrix material is easy to obtain, such as wheat bran, animal manure, grass ash, dried mango, corn cob, etc., which are readily available to farmers, and farmers can use
  • the substrate material is agricultural environmental protection raw material, no pollution
  • the traditional substrate uses pure fecal grass matrix
  • the matrix is added with peat soil to reduce the fecal grass.
  • Ingredients, shorten the production cycle, and the quality of cultivation is better than the traditional pure fecal substrate, which improves the yield and quality of the fungus.
  • the invention discloses a substrate cultivation method, which is simple in operation and convenient for popularization.
  • the embodiment of the invention provides a Flammulina velutipes cultivation substrate and a cultivation method thereof, and the matrix raw material comprises the following ratios by volume ratio: 5%-20% of wheat bran, 5%-20% of animal feces, 10%-30% of grass ash, dry 10%-15% of manggo, 15%-35% of peat soil, 5%-15% of corncob; the pH of the matrix is 5.8-8.0, the conductivity is 550-750 ⁇ s/cm, and the water holding capacity is 55%-70%.
  • the air porosity is 10% to 30%, the total porosity is 50% to 85%, and the bulk density is 0.15 to 0.7 g/cm 3 .
  • the wheat bran has a diameter of 0.1 mm to 7 mm, and the peat soil has a diameter of 5 mm to 15 mm.
  • the corn cob is granular and has a diameter of 3 mm to 10 mm.
  • the water has a pH of 5.0 to 7.4 and an electrical conductivity of 100 ⁇ s/cm to 150 ⁇ s/cm.
  • the animal manure comprises one or more components of dry chicken manure, dry sheep manure, dry cow dung, dry pig manure, and dry horse manure, which are specifically adjusted according to the supply requirements of the raw materials in the geographical area.
  • the cultivation method of the embodiment of the invention preparing the matrix: mixing the matrix raw materials uniformly, adding water and mixing well, holding water amount of 64% to 70%, and then sterilizing and sterilizing in a jar or a plastic bag.
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the stage of the germination. It is found that the infection of the bacteria should be treated or removed in time to prevent spread and spread;
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag. The black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the mushroom of the small cap. ;
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm. .
  • the matrix has a pH of 6.0 to 7.5, an electric conductivity of 650 to 700 ⁇ s/cm, a water holding capacity of 64% to 67%, an air porosity of 21% to 28%, a total porosity of 55% to 70%, and a bulk density of 0.25. ⁇ 0.50g/cm 3 .
  • the substrate cultivation method comprises a mushroom rod and a trampoline planting.
  • a better matrix formulation was obtained by comparing the diameters of the stalk diameter, the length of the stalk, the diameter of the cap, and the yield of the same planting area.
  • Flammulina velutipes cultivation substrate raw materials also include the following ratios: wheat bran 18%, animal feces 15%, grass ash 27%, dry awning 10%, peat soil 22%, corn cob 6%. .
  • the substrate had a pH of 7.8, an electrical conductivity of 660 ⁇ s/cm, a water holding capacity of 64%, an air porosity of 28%, a total porosity of 56%, and a bulk density of 0.3 g/cm3.
  • the animal feces contains 100% of dry chicken manure.
  • the matrix material is uniformly blended, mixed with water, and the water holding capacity is 64% to 70%, and then sterilized in a jar or a plastic bag.
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the bacteria stage, and find that the infection of the bacteria should be treated or removed in time to prevent spread and spread;
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag.
  • the black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the long stipe of the small cap. Flammulina.
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • Flammulina velutipes cultivation substrate raw materials also include the following ratios: wheat bran 6%, animal feces 11%, grass ash 33%, dry awning 15%, peat soil 21%, corn cob 14%.
  • the substrate had a pH of 7.9, a conductivity of 640 ⁇ s/cm, a water holding capacity of 66%, an air porosity of 23%, a total porosity of 60%, and a bulk density of 0.35 g/cm3.
  • the animal feces contains 100% of dry chicken manure.
  • Formulating the substrate mixing the matrix materials uniformly, adding water and mixing well, holding water amount of 64% to 70%, and then sterilizing and sterilizing in a jar or a plastic bag;
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the bacteria stage, and find that the infection of the bacteria should be treated or removed in time to prevent spread.
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag. The black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the mushroom of the small cap.
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • Flammulina velutipes cultivation substrate raw materials also include the following ratios: wheat bran 15%, animal feces 20%, grass ash 15%, dry awning 14%, peat soil 23%, corn cob 13%.
  • the substrate had a pH of 8.0, a conductivity of 670 ⁇ s/cm, a water holding capacity of 65%, an air porosity of 26%, a total porosity of 57%, and a bulk density of 0.4 g/cm3.
  • the animal feces contains 30% dry chicken manure, 30% dry cow dung, and 40% dry pig manure.
  • the matrix material is uniformly blended, mixed with water, and the water holding capacity is 64% to 70%, and then sterilized in a jar or a plastic bag.
  • strains purchase conventional Flammulina velutipes strains, when the temperature drops to 22 ° C -27 ° C, the strains are connected to the bottle or In the medium in the plastic bag, 60 to 70 bottles (bags) can be connected to each bottle of bacteria.
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the bacteria stage, and find that the infection of the bacteria should be treated or removed in time to prevent spread.
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag. The black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the mushroom of the small cap.
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • Flammulina velutipes cultivation substrate raw materials also include the following ratios: wheat bran 20%, animal feces 12%, grass ash 21%, dry awning 10%, peat soil 28%, corn cob 9%. .
  • the substrate had a pH of 7.9, an electrical conductivity of 660 ⁇ s/cm, a water holding capacity of 66%, an air porosity of 25%, a total porosity of 57%, and a bulk density of 0.4 g/cm3.
  • the animal feces contains 30% dry chicken manure, 30% dry cow dung, and 40% dry pig manure.
  • the matrix material is uniformly blended, mixed with water, and the water holding capacity is 64% to 70%, and then sterilized in a jar or a plastic bag.
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the bacteria stage, and find that the infection of the bacteria should be treated or removed in time to prevent spread.
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag.
  • the black cloth covers the doors and windows of the cultivation room to promote the growth of the stipe to obtain the length of the small cap. Flammulina velutipes.
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • Matrix Conventional wood chip medium contains 70% wood chips, 27% rice bran or wheat bran, 1% sucrose, 15% gypsum powder, and 1% calcium carbonate.
  • the matrix material is uniformly blended, mixed with water, and the water holding capacity is 64% to 70%, and then sterilized in a jar or a plastic bag.
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the bottle or bag After inoculation, the bottle or bag is moved into the culture chamber, and the temperature is controlled at 22 °C ⁇ 25 °C. After the mycelial growth spreads, the fermentation heat is generated due to the respiration of the hyphae, which is 3 °C ⁇ 4 °C higher than the room temperature.
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material.
  • the mycelium can Overgrown with whole bottles; due to the accumulation of bacteria in the cultivation process of Flammulina velutipes, it is necessary to carefully check the bacteria stage, and find that the infection of the bacteria should be treated or removed in time to prevent spread.
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag. The black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the mushroom of the small cap.
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • Matrix Conventional cottonseed hull medium contains 75% cottonseed husk, 22% rice bran or wheat husk, 1% sucrose, 1% calcium superphosphate, and 1% gypsum powder.
  • the matrix material is uniformly blended, mixed with water, and the water holding capacity is 64% to 70%, and then sterilized in a jar or a plastic bag.
  • strains purchase conventional Flammulina velutipes strains. When the temperature of the material drops to 22 °C-27 °C, the strains are connected to the medium in the bottle or plastic bag, and each bottle can be connected to 60-70 bottles (bags). ).
  • the room temperature can be 3 ° C ⁇ 4 ° C lower than the appropriate temperature for mycelial growth; 2 to 3 days after inoculation, the hyphae will start to germinate, and after 10 days, it can spread to the inside of the material, through 25- For 35 days, the mycelium can grow over the whole bottle; because the cultivation process of Flammulina velutipes is prone to bacteria, it should be carefully checked in the germinating stage, and the infection of the bacteria should be treated or removed in time to prevent spread.
  • the fungus should be immediately sprayed (that is, the old bacteria on the surface should be scraped off) to promote the formation of the primordium and fruiting bodies; at this time, it is necessary to strengthen the ventilation and maintain a certain amount of scattered light for the bud. to cultivate.
  • the temperature is controlled between 8 °C and 14 °C, the relative humidity of the air is maintained at 90% to 95%, and the ventilation is changed slowly; the 15 cm high kraft paper bag is used at the mouth of the bottle or the mouth of the bag. The black cloth covers the doors and windows of the cultivation room, and promotes the growth of the stipe to obtain the mushroom of the small cap.
  • Harvesting period The harvesting of the general stipe is as long as 13 to 20 cm, and the diameter of the cap is 2 to 3 cm.
  • test data of the growth index of Flammulina velutipes collected according to the above specific examples are as follows:
  • the diameter of the stalk and the length of the stalk of Example 1-4 can be seen from the table.
  • the diameter of the cap is better than that of the examples 5-6, and the yield of the same planting area of the examples 1-4 is also higher than that of the examples 5-6.
  • the strain data cultivated by the matrix formulated by the technical scheme of the present invention can be seen. It is superior to the conventional Flammulina velutipes cultivation substrate.
  • the matrix material in the technical scheme of the invention is easy to obtain for farmers, the ratioing method is simple, easy to operate, and the yield and quality of the Flammulina velutipes species are improved.
  • the invention discloses a substrate cultivation method, which is simple in operation and convenient for popularization.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mushroom Cultivation (AREA)

Abstract

La présente invention concerne un substrat de culture et un procédé de culture pour flammulina velutipes. Du son de blé, des déchets animaux, des cendres végétales, du dicranoptéris sec, un sol tourbeux et des épis de maïs sont sélectionnés en tant que matériaux substrats, et le sol tourbeux est un matériau substrat riche en matière organique. La période de production des plantations de champignons est raccourcie, la qualité de la culture est également supérieure à celle d'un substrat de fumier de paille pur classique, et le rendement et la qualité de la plantation de champignons peuvent être efficacement améliorés. Le procédé de culture comprend des opérations simples.
PCT/CN2016/099274 2016-09-19 2016-09-19 Substrat de culture et procédé de culture pour flammulina velutipes WO2018049662A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/099274 WO2018049662A1 (fr) 2016-09-19 2016-09-19 Substrat de culture et procédé de culture pour flammulina velutipes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/099274 WO2018049662A1 (fr) 2016-09-19 2016-09-19 Substrat de culture et procédé de culture pour flammulina velutipes

Publications (1)

Publication Number Publication Date
WO2018049662A1 true WO2018049662A1 (fr) 2018-03-22

Family

ID=61619023

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2016/099274 WO2018049662A1 (fr) 2016-09-19 2016-09-19 Substrat de culture et procédé de culture pour flammulina velutipes

Country Status (1)

Country Link
WO (1) WO2018049662A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109548563A (zh) * 2018-11-16 2019-04-02 山东友和菌业有限公司 一种金针菇高产量配方及培育方法
CN113475310A (zh) * 2021-08-05 2021-10-08 河南省农业科学院植物营养与资源环境研究所 一种控制工厂化袋栽白灵菇原基数目形成和原基分化发育的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1058691A (zh) * 1991-03-26 1992-02-19 福建农学院食用菌实验场 菌草代木代粮栽培食用菌方法
CN101366346A (zh) * 2008-07-31 2009-02-18 芜湖野树林生物科技有限公司 一种纯白金针菇的培育方法
CN101759458A (zh) * 2008-11-19 2010-06-30 赵春 一种金针菇混合料生料栽培法
CN105294351A (zh) * 2015-11-26 2016-02-03 南宁市金沙壮畜牧养殖有限责任公司 一种食用菌的床栽栽培方法
CN105379560A (zh) * 2015-11-26 2016-03-09 南宁市金沙壮畜牧养殖有限责任公司 一种食用菌栽培方法
CN105917964A (zh) * 2016-05-20 2016-09-07 安徽省天瑞园生态农业科技有限公司 一种金针菇的培育方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1058691A (zh) * 1991-03-26 1992-02-19 福建农学院食用菌实验场 菌草代木代粮栽培食用菌方法
CN101366346A (zh) * 2008-07-31 2009-02-18 芜湖野树林生物科技有限公司 一种纯白金针菇的培育方法
CN101759458A (zh) * 2008-11-19 2010-06-30 赵春 一种金针菇混合料生料栽培法
CN105294351A (zh) * 2015-11-26 2016-02-03 南宁市金沙壮畜牧养殖有限责任公司 一种食用菌的床栽栽培方法
CN105379560A (zh) * 2015-11-26 2016-03-09 南宁市金沙壮畜牧养殖有限责任公司 一种食用菌栽培方法
CN105917964A (zh) * 2016-05-20 2016-09-07 安徽省天瑞园生态农业科技有限公司 一种金针菇的培育方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109548563A (zh) * 2018-11-16 2019-04-02 山东友和菌业有限公司 一种金针菇高产量配方及培育方法
CN113475310A (zh) * 2021-08-05 2021-10-08 河南省农业科学院植物营养与资源环境研究所 一种控制工厂化袋栽白灵菇原基数目形成和原基分化发育的方法
CN113475310B (zh) * 2021-08-05 2023-10-27 河南省农业科学院植物营养与资源环境研究所 一种控制白灵菇原基数目形成和原基分化发育的方法

Similar Documents

Publication Publication Date Title
WO2018049685A1 (fr) Substrat de culture et procédé de culture de flammulina velutipes
CN103787732B (zh) 一种西瓜穴盘育苗基质及其制作方法
CN103708953B (zh) 一种蔬菜育苗基质及其制作方法
CN103214315B (zh) 一种含采石场渣土、畜粪、秸秆和木炭粉的绿化基质及其制备方法
CN103755445B (zh) 一种西瓜穴盘育苗基质及其应用
CN103168668A (zh) 一种利用农业废弃物制备瓜果类蔬菜育苗基质
CN107197707A (zh) 一种花卉培养土及其制备方法
CN105393835A (zh) 蔬菜育苗基质
CN102986513A (zh) 一种瓜果蔬菜的无土栽培基质
CN104054563B (zh) 一种实用高效无土栽培蔬菜和花卉营养基质材料的生产方法
CN102986419A (zh) 一种有机育苗基质
CN104782463A (zh) 一种有机生态型无土栽培技术
CN101926278A (zh) 一种草炭替代型茄果类蔬菜育苗基质配方
CN111742814A (zh) 一种低成本的番茄栽培基质及其制备方法和应用
CN102783358A (zh) 一种露天大田栽培球盖菇的方法
CN106416799A (zh) 一种蔬菜栽培基质
CN107853128A (zh) 一种以蔬菜废弃物发酵产物为肥源的叶类有机蔬菜栽培基质
CN104671920A (zh) 一种辣椒专用有机基质及其制备方法
CN104446757B (zh) 一种含发酵木屑的轻型基质及其制备方法
WO2018049663A1 (fr) Substrat de culture et procédé de culture pour champignons
CN103804039B (zh) 一种机插水稻育秧基质及其生产方法
KR101693317B1 (ko) 자연 활성 토양 개선방법
CN105191623A (zh) 一种棉花种苗的培育方法
CN103444419B (zh) 一种利用发酵床废料进行棉花育苗移栽的方法
WO2018049662A1 (fr) Substrat de culture et procédé de culture pour flammulina velutipes

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16916042

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 16916042

Country of ref document: EP

Kind code of ref document: A1