WO2018048856A1 - Procédé de radiomarquage efficace de biomolécules - Google Patents

Procédé de radiomarquage efficace de biomolécules Download PDF

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Publication number
WO2018048856A1
WO2018048856A1 PCT/US2017/050214 US2017050214W WO2018048856A1 WO 2018048856 A1 WO2018048856 A1 WO 2018048856A1 US 2017050214 W US2017050214 W US 2017050214W WO 2018048856 A1 WO2018048856 A1 WO 2018048856A1
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic device
biomolecule
droplet
labeled
prosthetic group
Prior art date
Application number
PCT/US2017/050214
Other languages
English (en)
Inventor
Noel S. HA
R. Michael Van Dam
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Publication of WO2018048856A1 publication Critical patent/WO2018048856A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/082—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins the peptide being a RGD-containing peptide
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/088—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins conjugates with carriers being peptides, polyamino acids or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B59/00—Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
    • C07B59/008—Peptides; Proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

La présente invention concerne un procédé de radiomarquage d'une biomolécule au moyen d'un dispositif microfluidique qui comprend le chargement d'une ou plusieurs gouttelettes contenant un groupe prosthétique marqué par 18F sur ou dans le dispositif microfluidique et l'évaporation des une ou plusieurs gouttelette(s) contenant le groupe prosthétique marqué par 18F. Une ou plusieurs gouttelettes contenant la biomolécule sont chargées sur ou dans le dispositif microfluidique et réagissent avec le groupe prosthétique marqué par 18F qui reste après évaporation. Le produit de réaction est ensuite collecté à partir du dispositif microfluidique. Dans un autre mode de réalisation, une gouttelette contenant des ions de métal radioactif ou un complexe de métal radioactif est chargée dans le dispositif microfluidique, évaporée et amenée à réagir avec une gouttelette contenant une biomolécule dérivée avec un groupe chélateur. Dans un autre mode de réalisation supplémentaire, une gouttelette contenant un sel marqué par 18F est chargée sur le dispositif microfluidique, évaporée et amenée à réagir avec une gouttelette contenant une biomolécule conjuguée à un site de marquage d'échange isotopique.
PCT/US2017/050214 2016-09-06 2017-09-06 Procédé de radiomarquage efficace de biomolécules WO2018048856A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662384079P 2016-09-06 2016-09-06
US62/384,079 2016-09-06

Publications (1)

Publication Number Publication Date
WO2018048856A1 true WO2018048856A1 (fr) 2018-03-15

Family

ID=61562144

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/050214 WO2018048856A1 (fr) 2016-09-06 2017-09-06 Procédé de radiomarquage efficace de biomolécules

Country Status (1)

Country Link
WO (1) WO2018048856A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100254899A1 (en) * 2007-02-27 2010-10-07 The United States Of America, As Represented By The Secretary, Dept Of Health And Human Services Radiolabeled affibody molecules
US20110220505A1 (en) * 2010-03-09 2011-09-15 Sparkle Power Inc. Droplet manipulations on ewod microelectrode array architecture
WO2015100498A1 (fr) * 2014-01-03 2015-07-09 British Columbia Cancer Agency Branch Compositions et méthodes pour l'imagerie du cancer
US20160015838A1 (en) * 2013-03-08 2016-01-21 University Of Southern California Usc Stevens Vinylsulfone-based 18F-labeling Compositions and Methods and Uses Thereof
US20160107951A1 (en) * 2009-10-15 2016-04-21 The Regents Of The University Of California Digital microfluidic platform for radiochemistry

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100254899A1 (en) * 2007-02-27 2010-10-07 The United States Of America, As Represented By The Secretary, Dept Of Health And Human Services Radiolabeled affibody molecules
US20160107951A1 (en) * 2009-10-15 2016-04-21 The Regents Of The University Of California Digital microfluidic platform for radiochemistry
US20110220505A1 (en) * 2010-03-09 2011-09-15 Sparkle Power Inc. Droplet manipulations on ewod microelectrode array architecture
US20160015838A1 (en) * 2013-03-08 2016-01-21 University Of Southern California Usc Stevens Vinylsulfone-based 18F-labeling Compositions and Methods and Uses Thereof
WO2015100498A1 (fr) * 2014-01-03 2015-07-09 British Columbia Cancer Agency Branch Compositions et méthodes pour l'imagerie du cancer

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CAI ET AL.: "A Thiol-Reactive 18F-Labeling Agent, N-[2-(4-18F-fluorobenzamido)ethyl]maleimide, and Synthesis of RGD Peptide-Based Tracer for PET Imaging of avb3 Integrin Expression", JOURNAL OF NUCLEAR MEDICINE, vol. 47, no. 7, 1 July 2006 (2006-07-01), pages 1172 - 1180, XP002470156 *
CHEN ET AL.: "Radiolabelling Diverse Positron Emission Tomography (PET) Tracers Using a Single Digital Microfluidic Reactor Chip", LAB ON A CHIP, vol. 14, 5 December 2013 (2013-12-05), pages 902 - 910, XP055494443 *
WRIGHT ET AL.: "Microfluidic Preparation of a 89Zr-Labeled Trastuzumab Single-Patient Dose", JOURNAL OF NUCLEAR MEDICINE, vol. 57, 14 January 2016 (2016-01-14), pages 747 - 752, XP055494441 *

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